SSGCID: Target Page

PlviB.00188.a

Casein kinase II, alpha subunit, putative

CENTER ID:	PlviB.00188.a
ORGANISM:	Plasmodium vivax Salvador I
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlviB.00188.a.B1.GE37130	full length		1	335

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PVX_091095
RefSeq:	XP_001615212.1
UniProt:	A5K4A7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSSSISKRI YIPKFYADVN IHKPKEYYDY DNLELQWNKP NRYEIMKKIG RGKYSEVFNG YDTEYNRPCA IKVLKPVKKK KIKREIKILQ NLHGGPNIIK LLDIVKDPVT KTPSLIFEYI NNIDFKTLYP KFTDKDIRYY IYQILKALDY CHSQGIMHRD VKPHNIMIDH ENKQIRLIDW GLAEFYHPGQ EYNVRVASRY YKGPELLIDL QLYDYSLDIW SLGCMLAGMI FKKEPFFCGH DNYDQLVKIA KVLGTDDLHA YLKKYNIKLK PHYLNILGEY ERKPWSHFLT QSNIDIAKDE VIDLIDKMLI YDHAKRIAPK EAMEHPYFSE VREQS

NT Sequence

atgtcttcga gctccatcag caagcggatc tacattccca agttctacgc tgatgtgaac atccataagc ccaaagaata ctacgactac gataacttgg agctgcaatg gaacaagccg aatcggtacg aaattatgaa gaagattggg aggggcaaat acagcgaagt gtttaacggc tacgacacgg agtataacag gccctgcgcc attaaggtac ttaaaccagt aaagaaaaaa aaaataaaga gagaaatcaa aattctgcaa aacttgcatg gaggcccaaa catcatcaag ctactagaca tagtcaaaga cccagtcacc aaaacgcctt cactcatttt tgaatacatt aataatatag acttcaaaac gctgtaccct aagttcaccg ataaggacat acgctattac atataccaaa ttttgaaggc actagattac tgccatagcc agggcatcat gcatagggat gtgaagccac ataacattat gattgaccac gagaataagc aaattagact catcgattgg gggctagctg agttttacca cccggggcag gaatacaacg tgagagtagc cagcagatat tacaaaggac cggaacttct aatagatcta cagctgtatg attactctct agacatttgg agcttaggat gtatgctggc tgggatgata tttaaaaagg agcctttttt ttgtggccac gataactacg atcagcttgt taagattgcc aaagtgctag gcactgatga tcttcatgcc tacttaaaaa aatacaacat aaaattgaag ccccattatt taaacatcct gggggagtac gagcgcaaac cctggtccca cttcctcacg cagtcaaaca ttgacattgc caaggatgag gtgatcgacc tcatcgataa gatgcttata tacgaccacg ccaagaggat cgcccccaag gaggccatgg agcaccccta cttcagcgag gtccgcgagc agtcctga

Details for PlviB.00188.a.B1.GE37130

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	88
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMS SSSISKRIYI PKFYADVNIH KPKEYYDYDN LELQWNKPSR YEIMKKIGRG KYSEVFNGYD TEYNRPCAIK VLKPVKKKKI KREIKILQNL HGGPNIIKLL DIVKDPVTKT PSLIFEYINN IDFKTLYPKF TDKDIRYYIY QILKALDYCH SQGIMHRDVK PHNIMIDHEN KQIRLVDWGL AEFYHPGQEY NVRVASRYYK GPELLIDLQL YDYSLDIWSL GCMLAGMIFK KEPFFCGHDN YDQLVKIXKV LGTDDLHAYL KKYNIKLKPH YLXXLGEYER KPWSHFLTQS NIDIAXDXXS TXRXXLIYXX XXSPXXXXXP XXAXXXXXXX X

Validated NT Sequence

tttgnnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgtcttc gagctccatc agcaagcgga tctacattcc caagttctac gctgatgtga acatccataa gcccaaagaa tactacgact acgataactt ggagctgcaa tggaacaagc cgagtcggta cgaaattatg aagaagattg ggaggggcaa atacagcgaa gtgtttaacg gctacgacac ggagtataac aggccctgcg ccattaaggt acttaaacca gtaaagaaaa aaaaaataaa gagagaaatc aaaattctgc aaaacttgca tggaggccca aacatcatca agctactaga catagtcaaa gacccagtca ccaaaacgcc ttcactcatt tttgaataca ttaataatat agacttcaaa acgctgtacc ctaagttcac cgataaggac atacgctatt acatatacca aattttgaag gcactagatt actgccatag ccagggcatc atgcataggg atgtgaagcc acataacatt atgattgacc acgagaataa gcaaattaga ctcgtcgatt gggggctagc tgagttttac cacccggggc aggaatacaa cgtgagagta gccagcagat attacaaagg accggaactt ctaatagatc tacagctgta tgattactct ctagacattt ggagcttagg atgtatgctg gctgggatga tatttaaaaa ggagcctttt ttttgtggcc acgataacta cgatcagctt gttaagattg ncaaagtgct aggcactgat gatcttcatg cctacttaaa aaaatacaac ataaaattga agccccatta tttaaanntc ctgggggagt acgagcgcaa accctggtcc cacttcctca cgcagtcaaa cattgacatt gccanggatg anngatcgac nnntcganan nngcttatat acnacnnnnn nnnntcgccc cnnnnnnnnn nnncacccna nnnngcnagn cnnnnnnagn ctannnnnta nna

Expected Protein Sequence

MAHHHHHHMS SSSISKRIYI PKFYADVNIH KPKEYYDYDN LELQWNKPNR YEIMKKIGRG KYSEVFNGYD TEYNRPCAIK VLKPVKKKKI KREIKILQNL HGGPNIIKLL DIVKDPVTKT PSLIFEYINN IDFKTLYPKF TDKDIRYYIY QILKALDYCH SQGIMHRDVK PHNIMIDHEN KQIRLIDWGL AEFYHPGQEY NVRVASRYYK GPELLIDLQL YDYSLDIWSL GCMLAGMIFK KEPFFCGHDN YDQLVKIAKV LGTDDLHAYL KKYNIKLKPH YLNILGEYER KPWSHFLTQS NIDIAKDEVI DLIDKMLIYD HAKRIAPKEA MEHPYFSEVR EQS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt cgagctccat cagcaagcgg atctacattc ccaagttcta cgctgatgtg aacatccata agcccaaaga atactacgac tacgataact tggagctgca atggaacaag ccgaatcggt acgaaattat gaagaagatt gggaggggca aatacagcga agtgtttaac ggctacgaca cggagtataa caggccctgc gccattaagg tacttaaacc agtaaagaaa aaaaaaataa agagagaaat caaaattctg caaaacttgc atggaggccc aaacatcatc aagctactag acatagtcaa agacccagtc accaaaacgc cttcactcat ttttgaatac attaataata tagacttcaa aacgctgtac cctaagttca ccgataagga catacgctat tacatatacc aaattttgaa ggcactagat tactgccata gccagggcat catgcatagg gatgtgaagc cacataacat tatgattgac cacgagaata agcaaattag actcatcgat tgggggctag ctgagtttta ccacccgggg caggaataca acgtgagagt agccagcaga tattacaaag gaccggaact tctaatagat ctacagctgt atgattactc tctagacatt tggagcttag gatgtatgct ggctgggatg atatttaaaa aggagccttt tttttgtggc cacgataact acgatcagct tgttaagatt gccaaagtgc taggcactga tgatcttcat gcctacttaa aaaaatacaa cataaaattg aagccccatt atttaaacat cctgggggag tacgagcgca aaccctggtc ccacttcctc acgcagtcaa acattgacat tgccaaggat gaggtgatcg acctcatcga taagatgctt atatacgacc acgccaagag gatcgccccc aaggaggcca tggagcaccc ctacttcagc gaggtccgcg agcagtcctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat