PlviB.00043.a

Dihydroorotase, putative

CENTER ID: PlviB.00043.a
ORGANISM: Plasmodium vivax Salvador I
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlviB.00043.a.B1.GE37127 full length 1 361
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PVX_116830
RefSeq: XP_001615660.1
UniProt: A5K330

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEPLHLPLGD DMHCHLRQGD MLRFTVGAVK QGGCDRVLVM PNITPIVSTC EDAKKYRSEL TKQDPSVDYL MTLYLNNKTD ANDILRNHEE CNLQGVKIYP SNVTTNSSHG VTTLETYYEI FHTLERLNKS LHIHCEEPNV NPLYAERSYA QHIHDLALHF PHLKIVLEHI STVDMINLVR TYPNIAGSVT PHHLQLTIDD VVDVESYDYA PDVVTIEKYI KNVYNYCKPL PKTIDDKVAL CKIIQEGFPR IFLGSDSAPH YKEQKNDPHY KPGIYTQPFL MSYVSHIFNK MNSLDKVENF ACKNAANFLN LKEKKTGTNL QPLSLCIQKK ELKIADEYSG VVPFLAGQTI DFTATCVGAD V
NT Sequence
atggaaccgc tgcacctccc cctgggcgac gacatgcact gccacctgcg gcagggcgac atgctgcgct tcaccgttgg ggcagtcaag caggggggct gcgaccgcgt gctagtcatg cccaacatca cgccaatcgt gagcacctgt gaagatgcca aaaaatatag aagcgagcta accaagcagg accccagcgt agactaccta atgacgttat atttgaataa taaaacagac gcaaatgaca tactcagaaa tcacgaagag tgtaatttgc aaggagtaaa aatatacccc agcaatgtga cgaccaactc gagtcatgga gttaccacgt tagaaactta ttatgaaata tttcatacac ttgaaaggtt aaacaaaagt ctacacatcc attgtgaaga accaaatgtg aatccccttt atgcagaaag gagttacgca caacatattc atgacttggc tcttcacttc ccccatttga aaattgtcct agagcacatc tctacggtag atatgataaa cctggttagg acgtacccga acatcgcggg gtcagttact ccgcaccact tgcaactaac catagacgat gttgtagatg tggagagcta cgattatgct cccgacgtgg taaccataga gaagtatata aaaaatgtgt ataattattg caaacctttg cccaaaacga ttgatgataa ggtagctttg tgtaagatca tacaggaagg gttccccagg attttccttg gatccgattc tgcgccccat tataaagagc aaaaaaatga cccccactat aagccaggga tatacacaca gccattcctc atgtcgtacg tttctcacat ttttaacaaa atgaattcat tggataaggt agaaaatttt gcctgcaaaa atgctgcaaa ttttttaaat ttaaaggaga aaaaaacggg cacaaatcta cagcctctct ctctttgtat acaaaagaaa gaactcaaaa ttgctgatga atattctggg gtcgtcccct ttttggctgg acaaacaatt gattttacag cgacgtgtgt gggggcagat gtttga
Details for PlviB.00043.a.B1.GE37127
HARVESTED ON: 7/17/2013
SEQUENCED ON: 7/22/2013
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHME PLHLPLGDDM HCHLRQGDML RFTVGAVKQG GCDRVLVMPN ITPIVSTCED AKKYRSELTK QDPSVDYLMT LYLNNKTDAN DILKNHEECN LQGVKIYPSN VTTNSSHGVT TLETYYEIFH TLERLNKSLH IHCEEPNVNP LYAERSYAQH IHDLALHFPH LKIVLEHIST VDMINLVRTY PNIAGSVTPH HSQLTIDDVV DMESYDYAPD VVTIEKYIKN VYNYCKPLPK TIDDKVALCK IIQEGFPRXF LGSXSAPHYK EQKNDPHYKP GXYTQPFLMS YVSHIFNKMN SLXXXENFAC KMLQIF
Validated NT Sequence
ttttgttnnn tttaagaagg aganatacca tggctcacca ccaccaccac catatggaac cgctgcacct ccccctgggc gacgacatgc actgccacct gcggcagggc gacatgctgc gcttcaccgt tggggcagtc aagcaggggg gctgcgaccg cgtgctagtc atgcccaaca tcacgccaat cgtgagcacc tgtgaagatg ccaaaaaata tagaagcgag ctaaccaagc aggaccccag cgtagactac ctaatgacgt tatatttgaa taataaaaca gacgcaaatg acatactcaa aaatcacgaa gagtgtaatt tgcaaggagt aaaaatatac cccagcaatg tgacgaccaa ctcgagtcat ggagttacca cgttagaaac ttattatgaa atatttcata cacttgaaag gttaaacaaa agtctacaca tccattgtga agaaccaaat gtgaatcccc tttatgcaga aaggagttac gcacaacata ttcatgactt ggctcttcac ttcccccatt tgaaaattgt cctagagcac atctctacgg tagatatgat aaacctggtt aggacgtacc cgaacatcgc ggggtcagtt actccgcacc actcgcaact aaccatagac gatgttgtag atatggagag ctacgattat gctcccgacg tggtaaccat agagaagtat ataaaaaatg tgtataatta ttgcaaacct ttgcccaaaa cgattgatga taaggtagct ttgtgtaaga tcatacagga agggttcccc aggantttcc ttggatccga ntctgcgccc cattataaag agcaaaaaaa tgacccccac tataagccag gnnnatacac acagccattc ctcatgtcgt acgtttctca catttttaac aaaatgaatt cattggntaa nnnagaaaat tttgcctgca aaatgctgca aattttttaa atttaaagga gaaaaaangg gnanaaatct acngcctctc nctcnttnnn atnnaaanna aannactcaa annnntgang annntttnng ggncnncccn tttnnnngnn naannnnnat tnnnngcnnn nnnnngggnn nnnnnttnnn annnnnnnnn nnnngctann nannnnnnnn nnnnnnnnnn nnncnncnnn nnnnnnnnnn nnnnnnnnnn nncnnnnnnn nnnnnnnnnn annnanna
Expected Protein Sequence
MAHHHHHHME PLHLPLGDDM HCHLRQGDML RFTVGAVKQG GCDRVLVMPN ITPIVSTCED AKKYRSELTK QDPSVDYLMT LYLNNKTDAN DILRNHEECN LQGVKIYPSN VTTNSSHGVT TLETYYEIFH TLERLNKSLH IHCEEPNVNP LYAERSYAQH IHDLALHFPH LKIVLEHIST VDMINLVRTY PNIAGSVTPH HLQLTIDDVV DVESYDYAPD VVTIEKYIKN VYNYCKPLPK TIDDKVALCK IIQEGFPRIF LGSDSAPHYK EQKNDPHYKP GIYTQPFLMS YVSHIFNKMN SLDKVENFAC KNAANFLNLK EKKTGTNLQP LSLCIQKKEL KIADEYSGVV PFLAGQTIDF TATCVGADV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac cgctgcacct ccccctgggc gacgacatgc actgccacct gcggcagggc gacatgctgc gcttcaccgt tggggcagtc aagcaggggg gctgcgaccg cgtgctagtc atgcccaaca tcacgccaat cgtgagcacc tgtgaagatg ccaaaaaata tagaagcgag ctaaccaagc aggaccccag cgtagactac ctaatgacgt tatatttgaa taataaaaca gacgcaaatg acatactcag aaatcacgaa gagtgtaatt tgcaaggagt aaaaatatac cccagcaatg tgacgaccaa ctcgagtcat ggagttacca cgttagaaac ttattatgaa atatttcata cacttgaaag gttaaacaaa agtctacaca tccattgtga agaaccaaat gtgaatcccc tttatgcaga aaggagttac gcacaacata ttcatgactt ggctcttcac ttcccccatt tgaaaattgt cctagagcac atctctacgg tagatatgat aaacctggtt aggacgtacc cgaacatcgc ggggtcagtt actccgcacc acttgcaact aaccatagac gatgttgtag atgtggagag ctacgattat gctcccgacg tggtaaccat agagaagtat ataaaaaatg tgtataatta ttgcaaacct ttgcccaaaa cgattgatga taaggtagct ttgtgtaaga tcatacagga agggttcccc aggattttcc ttggatccga ttctgcgccc cattataaag agcaaaaaaa tgacccccac tataagccag ggatatacac acagccattc ctcatgtcgt acgtttctca catttttaac aaaatgaatt cattggataa ggtagaaaat tttgcctgca aaaatgctgc aaatttttta aatttaaagg agaaaaaaac gggcacaaat ctacagcctc tctctctttg tatacaaaag aaagaactca aaattgctga tgaatattct ggggtcgtcc cctttttggc tggacaaaca attgatttta cagcgacgtg tgtgggggca gatgtttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t