PlviA.18517.a

Elongation factor 1B, putative

CENTER ID: PlviA.18517.a
ORGANISM: Plasmodium vivax
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlviA.18517.a.B1.GE36379 full length 1 280
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlviA.18517.a.B1.PW37242 full length 1 280
PlviA.18517.a.B1.PW37243 full length 1 280

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PVX_099095
OrthoMCL: OG5_127390
RefSeq: XP_001614668.1
UniProt: A5K6K0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASNANLLTV KGEGDYGKLN AFFAEHSYYQ NHMLSAMDLK IFNQIKCVVS KDTYPHLYRW YKHIDSLPSY VTDQFKDPSE KGKKAAGSHA PEKKSTPEDD DVDNDIDLFG DSNNDDQNIL LEKKKQKEEE LKKKKQKEKE KNRSILIIEI KPKSIDTDIA KIPKLVKQKI VDENIKWGEE VKKLPVAFGL YKLHMSCIIY DDFVNTNDLM EKIENIDLEN EEDKKKRKLI LGLDGEVNGG NSDEEGDDDG ANEDDVNYED VEDDLDFLVQ SAEIITFNKL
NT Sequence
atggctagca acgcaaattt attaactgta aaaggcgaag gggactatgg caaactgaac gcctttttcg ccgagcactc gtactaccaa aaccacatgc taagtgccat ggatttgaaa atctttaatc aaataaaatg tgtggtaagt aaagatacat acccccattt gtaccgttgg tataagcaca tcgactcgtt gcccagttat gtcacagacc aatttaagga tcccagcgaa aaggggaaaa aagctgcagg tagccacgca ccagagaaaa aaagcacccc agaagatgat gatgttgata acgatattga cctattcggg gacagcaata atgatgacca gaacatcctc ttagagaaaa aaaaacagaa agaagaagaa ttaaagaaga agaagcaaaa ggaaaaggag aaaaacaggt ccattttaat tatcgaaata aagcccaaat caattgacac agatattgct aaaatcccca aactggttaa gcaaaaaatt gtggatgaaa atatcaaatg gggtgaggaa gtaaaaaaat tacccgtagc ttttggcctg tacaaacttc acatgtcatg tattatctat gacgactttg tcaatacgaa tgacttgatg gaaaagatag aaaatataga tttggaaaat gaggaggaca agaaaaagag gaagctgatt ctcgggctgg atggagaggt aaacggcggc aacagtgacg aagagggcga cgacgatggg gctaatgagg acgacgtgaa ttacgaagac gtggaggacg acctggactt cctggtgcag tcggccgaga ttatcacctt caacaagttg tga
Details for PlviA.18517.a.B1.GE36379
HARVESTED ON: 10/23/2012
SEQUENCED ON: 10/26/2012
EXPECTED MW: 33kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 96
PERCENT COVERAGE: 47
Validated AA Sequence
MAHHHHHHMA SNANLLTVKG EGDYGKLNAF FAEHSYYQNH MLSAMDLKIF NQIKCVVSKD TYPHLYRWYK HIDSLPSYVT DQFKDPSEKG KKAAGSHAPE KKSTPEDDDV DNDIDLFGDS NNDDQNILLE KKNRKKKN
Validated NT Sequence
atcttactca cttacaactt gttgaaggtg ataatctcgg ccgactgcac caggaagtcc aggtcgtcct ccacgtcttc gtaattcacg tcgtcgccct cttcgtcact gttgccgccg tttacctctc catccagccc gagaatcagc ttcctctttt tcttgtcctc ctcattttcc aaatctatat tttctatctt ttccatcaag tcattcgtat tgacaaagtc gtcatagata atacatgaca tgtgaagttt gtacaggcca aaagctacgg gtaatttttt tacttcctca ccccatttga tattttcatc cacaattttt tgcttaacca gtttggggat tttagcaata tctgtgtcaa ttgatttggg ctttatttcg ataattaaaa tggacctgtt tttctccttt tccttttgct tcttcttctt taattcttct tctttctgtt ttttttctct aagaggatgt tctggtcatc attattgctg tccccgaata ggtcaatatc gttatcaaca tcatcatctt ctggggtgct ttttttctct ggtgcgtggc tacctgcagc ttttttcccc ttttcgctgg gatccttaaa ctggtctgtg acataactgg gcaacgagtc gatgtgctta taccaacggt acaaatgggg gtatgtatct ttacttacca cacattttat ttgattaaag attttcaaat ccatggcact tagcatgtgg ttttggtagt acgagtgctc ggcgaaaaag gcgttcagtt tgccatagtc cccttcgcct tttacggtta ataaatttgc gttgctagcc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMA SNANLLTVKG EGDYGKLNAF FAEHSYYQNH MLSAMDLKIF NQIKCVVSKD TYPHLYRWYK HIDSLPSYVT DQFKDPSEKG KKAAGSHAPE KKSTPEDDDV DNDIDLFGDS NNDDQNILLE KKKQKEEELK KKKQKEKEKN RSILIIEIKP KSIDTDIAKI PKLVKQKIVD ENIKWGEEVK KLPVAFGLYK LHMSCIIYDD FVNTNDLMEK IENIDLENEE DKKKRKLILG LDGEVNGGNS DEEGDDDGAN EDDVNYEDVE DDLDFLVQSA EIITFNKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta gcaacgcaaa tttattaact gtaaaaggcg aaggggacta tggcaaactg aacgcctttt tcgccgagca ctcgtactac caaaaccaca tgctaagtgc catggatttg aaaatcttta atcaaataaa atgtgtggta agtaaagata cataccccca tttgtaccgt tggtataagc acatcgactc gttgcccagt tatgtcacag accaatttaa ggatcccagc gaaaagggga aaaaagctgc aggtagccac gcaccagaga aaaaaagcac cccagaagat gatgatgttg ataacgatat tgacctattc ggggacagca ataatgatga ccagaacatc ctcttagaga aaaaaaaaca gaaagaagaa gaattaaaga agaagaagca aaaggaaaag gagaaaaaca ggtccatttt aattatcgaa ataaagccca aatcaattga cacagatatt gctaaaatcc ccaaactggt taagcaaaaa attgtggatg aaaatatcaa atggggtgag gaagtaaaaa aattacccgt agcttttggc ctgtacaaac ttcacatgtc atgtattatc tatgacgact ttgtcaatac gaatgacttg atggaaaaga tagaaaatat agatttggaa aatgaggagg acaagaaaaa gaggaagctg attctcgggc tggatggaga ggtaaacggc ggcaacagtg acgaagaggg cgacgacgat ggggctaatg aggacgacgt gaattacgaa gacgtggagg acgacctgga cttcctggtg cagtcggccg agattatcac cttcaacaag ttgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PlviA.18517.a.B1.PW37242
PURIFICATION DATe: 2/6/2013
CONCENTRATION: 22mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 47
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA SNANLLTVKG EGDYGKLNAF FAEHSYYQNH MLSAMDLKIF NQIKCVVSKD TYPHLYRWYK HIDSLPSYVT DQFKDPSEKG KKAAGSHAPE KKSTPEDDDV DNDIDLFGDS NNDDQNILLE KKNRKKKN
Validated NT Sequence
atcttactca cttacaactt gttgaaggtg ataatctcgg ccgactgcac caggaagtcc aggtcgtcct ccacgtcttc gtaattcacg tcgtcgccct cttcgtcact gttgccgccg tttacctctc catccagccc gagaatcagc ttcctctttt tcttgtcctc ctcattttcc aaatctatat tttctatctt ttccatcaag tcattcgtat tgacaaagtc gtcatagata atacatgaca tgtgaagttt gtacaggcca aaagctacgg gtaatttttt tacttcctca ccccatttga tattttcatc cacaattttt tgcttaacca gtttggggat tttagcaata tctgtgtcaa ttgatttggg ctttatttcg ataattaaaa tggacctgtt tttctccttt tccttttgct tcttcttctt taattcttct tctttctgtt ttttttctct aagaggatgt tctggtcatc attattgctg tccccgaata ggtcaatatc gttatcaaca tcatcatctt ctggggtgct ttttttctct ggtgcgtggc tacctgcagc ttttttcccc ttttcgctgg gatccttaaa ctggtctgtg acataactgg gcaacgagtc gatgtgctta taccaacggt acaaatgggg gtatgtatct ttacttacca cacattttat ttgattaaag attttcaaat ccatggcact tagcatgtgg ttttggtagt acgagtgctc ggcgaaaaag gcgttcagtt tgccatagtc cccttcgcct tttacggtta ataaatttgc gttgctagcc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMA SNANLLTVKG EGDYGKLNAF FAEHSYYQNH MLSAMDLKIF NQIKCVVSKD TYPHLYRWYK HIDSLPSYVT DQFKDPSEKG KKAAGSHAPE KKSTPEDDDV DNDIDLFGDS NNDDQNILLE KKKQKEEELK KKKQKEKEKN RSILIIEIKP KSIDTDIAKI PKLVKQKIVD ENIKWGEEVK KLPVAFGLYK LHMSCIIYDD FVNTNDLMEK IENIDLENEE DKKKRKLILG LDGEVNGGNS DEEGDDDGAN EDDVNYEDVE DDLDFLVQSA EIITFNKL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTA GCAACGCAAA TTTATTAACT GTAAAAGGCG AAGGGGACTA TGGCAAACTG AACGCCTTTT TCGCCGAGCA CTCGTACTAC CAAAACCACA TGCTAAGTGC CATGGATTTG AAAATCTTTA ATCAAATAAA ATGTGTGGTA AGTAAAGATA CATACCCCCA TTTGTACCGT TGGTATAAGC ACATCGACTC GTTGCCCAGT TATGTCACAG ACCAATTTAA GGATCCCAGC GAAAAGGGGA AAAAAGCTGC AGGTAGCCAC GCACCAGAGA AAAAAAGCAC CCCAGAAGAT GATGATGTTG ATAACGATAT TGACCTATTC GGGGACAGCA ATAATGATGA CCAGAACATC CTCTTAGAGA AAAAAAAACA GAAAGAAGAA GAATTAAAGA AGAAGAAGCA AAAGGAAAAG GAGAAAAACA GGTCCATTTT AATTATCGAA ATAAAGCCCA AATCAATTGA CACAGATATT GCTAAAATCC CCAAACTGGT TAAGCAAAAA ATTGTGGATG AAAATATCAA ATGGGGTGAG GAAGTAAAAA AATTACCCGT AGCTTTTGGC CTGTACAAAC TTCACATGTC ATGTATTATC TATGACGACT TTGTCAATAC GAATGACTTG ATGGAAAAGA TAGAAAATAT AGATTTGGAA AATGAGGAGG ACAAGAAAAA GAGGAAGCTG ATTCTCGGGC TGGATGGAGA GGTAAACGGC GGCAACAGTG ACGAAGAGGG CGACGACGAT GGGGCTAATG AGGACGACGT GAATTACGAA GACGTGGAGG ACGACCTGGA CTTCCTGGTG CAGTCGGCCG AGATTATCAC CTTCAACAAG TTGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for PlviA.18517.a.B1.PW37243
PURIFICATION DATe: 2/6/2013
CONCENTRATION: 36.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 47
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA SNANLLTVKG EGDYGKLNAF FAEHSYYQNH MLSAMDLKIF NQIKCVVSKD TYPHLYRWYK HIDSLPSYVT DQFKDPSEKG KKAAGSHAPE KKSTPEDDDV DNDIDLFGDS NNDDQNILLE KKNRKKKN
Validated NT Sequence
atcttactca cttacaactt gttgaaggtg ataatctcgg ccgactgcac caggaagtcc aggtcgtcct ccacgtcttc gtaattcacg tcgtcgccct cttcgtcact gttgccgccg tttacctctc catccagccc gagaatcagc ttcctctttt tcttgtcctc ctcattttcc aaatctatat tttctatctt ttccatcaag tcattcgtat tgacaaagtc gtcatagata atacatgaca tgtgaagttt gtacaggcca aaagctacgg gtaatttttt tacttcctca ccccatttga tattttcatc cacaattttt tgcttaacca gtttggggat tttagcaata tctgtgtcaa ttgatttggg ctttatttcg ataattaaaa tggacctgtt tttctccttt tccttttgct tcttcttctt taattcttct tctttctgtt ttttttctct aagaggatgt tctggtcatc attattgctg tccccgaata ggtcaatatc gttatcaaca tcatcatctt ctggggtgct ttttttctct ggtgcgtggc tacctgcagc ttttttcccc ttttcgctgg gatccttaaa ctggtctgtg acataactgg gcaacgagtc gatgtgctta taccaacggt acaaatgggg gtatgtatct ttacttacca cacattttat ttgattaaag attttcaaat ccatggcact tagcatgtgg ttttggtagt acgagtgctc ggcgaaaaag gcgttcagtt tgccatagtc cccttcgcct tttacggtta ataaatttgc gttgctagcc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMA SNANLLTVKG EGDYGKLNAF FAEHSYYQNH MLSAMDLKIF NQIKCVVSKD TYPHLYRWYK HIDSLPSYVT DQFKDPSEKG KKAAGSHAPE KKSTPEDDDV DNDIDLFGDS NNDDQNILLE KKKQKEEELK KKKQKEKEKN RSILIIEIKP KSIDTDIAKI PKLVKQKIVD ENIKWGEEVK KLPVAFGLYK LHMSCIIYDD FVNTNDLMEK IENIDLENEE DKKKRKLILG LDGEVNGGNS DEEGDDDGAN EDDVNYEDVE DDLDFLVQSA EIITFNKL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTA GCAACGCAAA TTTATTAACT GTAAAAGGCG AAGGGGACTA TGGCAAACTG AACGCCTTTT TCGCCGAGCA CTCGTACTAC CAAAACCACA TGCTAAGTGC CATGGATTTG AAAATCTTTA ATCAAATAAA ATGTGTGGTA AGTAAAGATA CATACCCCCA TTTGTACCGT TGGTATAAGC ACATCGACTC GTTGCCCAGT TATGTCACAG ACCAATTTAA GGATCCCAGC GAAAAGGGGA AAAAAGCTGC AGGTAGCCAC GCACCAGAGA AAAAAAGCAC CCCAGAAGAT GATGATGTTG ATAACGATAT TGACCTATTC GGGGACAGCA ATAATGATGA CCAGAACATC CTCTTAGAGA AAAAAAAACA GAAAGAAGAA GAATTAAAGA AGAAGAAGCA AAAGGAAAAG GAGAAAAACA GGTCCATTTT AATTATCGAA ATAAAGCCCA AATCAATTGA CACAGATATT GCTAAAATCC CCAAACTGGT TAAGCAAAAA ATTGTGGATG AAAATATCAA ATGGGGTGAG GAAGTAAAAA AATTACCCGT AGCTTTTGGC CTGTACAAAC TTCACATGTC ATGTATTATC TATGACGACT TTGTCAATAC GAATGACTTG ATGGAAAAGA TAGAAAATAT AGATTTGGAA AATGAGGAGG ACAAGAAAAA GAGGAAGCTG ATTCTCGGGC TGGATGGAGA GGTAAACGGC GGCAACAGTG ACGAAGAGGG CGACGACGAT GGGGCTAATG AGGACGACGT GAATTACGAA GACGTGGAGG ACGACCTGGA CTTCCTGGTG CAGTCGGCCG AGATTATCAC CTTCAACAAG TTGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT