PlviA.00425.a

Elongation factor 1-alpha

CENTER ID: PlviA.00425.a
ORGANISM: Plasmodium vivax
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlviA.00425.a.B1.GE36149 full length 1 443
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PVX_114830
RefSeq: XP_001616354.1
RefSeq: XP_001616355.1
UniProt: A5K2H7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKEKTHINL VVIGHVDSGK STTTGHIIYK LGGIDRRTIE KFEKESAEMG KGSFKYAWVL DKLKAERERG ITIDIALWKF ETPRYFFTVI DAPGHKDFIK NMITGTSQAD VALLVVPAEV GGFEGAFSKE GQTKEHALLA FTLGVKQIVV GVNKMDTVKY SEDRYEEIKK EVRDYLKKVG YQADKVDFIP ISGFEGDNLI EKSDKTPWYK GRTLIEALDT MEPPKRPYDK PLRIPLQGVY KIGGIGTVPV GRVETGILKA GMVLNFAPSA VVSECKSVEM HKEVLEEARP GDNIGFNVKN VSVKEIKRGY VASDTKNEPA KGCSKFTAQV IILNHPGEIK NGYSPVLDCH TAHISCKFLN IDSKIDKRSG KVVEENPKSI KSGDSALVSL EPKKPMVVET FTEYPPLGRF AIRDMRQTIA VGIIKAVEKK EPGAVSAKNP AKK
NT Sequence
atgggaaagg aaaaaacaca tatcaactta gtcgttatcg gccatgtcga tagtggaaaa tccacgacca ccggtcatat tatttacaag ttgggaggta ttgacaggag aaccatagaa aagtttgaga aagagtccgc cgaaatgggt aagggtagtt ttaagtacgc gtgggtgttg gataagctaa aggccgaaag agaaagaggt atcactatcg atatcgcctt atggaagttc gaaacgccca ggtacttctt cacagtcatt gatgcccccg gtcataagga ttttatcaaa aatatgatta cgggtacgtc gcaggctgat gtggctctct tggttgttcc tgctgaggtt ggtggttttg aaggtgcctt ttcaaaggaa ggtcaaacta aggaacacgc cttgttagcc ttcaccctcg gtgtgaaaca gatcgttgtt ggtgtcaaca agatggacac tgtgaaatac tcagaagata gatacgaaga aattaagaag gaagttaggg attacctgaa aaaggtcgga taccaagctg acaaggttga ctttatcccc atttctggat ttgaaggtga taacctgatt gagaagtcag acaagacccc atggtataaa ggaaggacct tgatcgaggc tttggacact atggaaccac ccaagagacc atacgacaaa cctttaagaa ttccactcca aggtgtgtac aaaattggtg gtattggtac cgtgcccgtt ggcagagtcg aaactggtat tttgaaggcc ggtatggttc tcaactttgc cccatctgct gtcgtttcgg agtgtaaatc cgtcgaaatg cacaaagaag ttttggaaga ggcaagacca ggtgacaaca tcggatttaa cgtaaagaac gtttctgtta aagaaatcaa gagaggatat gtcgcttctg ataccaagaa tgagccagcc aagggatgct ccaaatttac tgcccaggtt atcattttga accatcctgg agaaattaag aacggataca gtcccgtcct agattgccac actgctcaca tttcttgcaa atttttgaac atcgactcga agattgacaa gcgttctggt aaggtcgttg aggaaaaccc taaatcaatt aagtctggtg attcagcctt ggttagtctc gagccaaaga agcctatggt tgttgagacc ttcactgagt atccacccct aggaagattt gctattcgag atatgagaca aaccatcgct gtcggtatca tcaaggcggt cgaaaagaag gagccaggtg ccgtgtcagc caaaaaccca gcaaagaaat ga
Details for PlviA.00425.a.B1.GE36149
HARVESTED ON: 9/26/2012
SEQUENCED ON: 10/23/2013
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG KEKTHINLVV IGHVDSGKST TTGHIIYKLG GIDRRTIEKF EKESAEMGKG SFKYAWVLDK LKAERERGIT IDIALWKFET PRYFFTVIDA PGHKDFIKNM ITGTSQADVA LLVVPAEVGG FEGAFSKEGQ TKEHALLAFT LGVKQIVVGV NKMDTVKYSE DRYEEIKKEV RDYLKKVGYQ ADKVDFIPIS GFEGDNLIEK SDKTPWYKGR TLIEASDTME PPKRPYDKPL RIPLQGVYKI GGIGTVPVGR VETGILKAGM VLNFAPSAVV SECKSVEMHK EVLEEARPGD NIGFNVKNVS VKEIKRGYVA SDTKNEPAKG CSKFTAQVII LNHPGEIKNG YSPVLDCHTA HISCKFLNID SKIDKRSGKV VEENPKSIKS GDSALVSLEP KKPMVVETFT EYPPLGRFAI RDMRQTVAVG IIKAVEKKEP G
Validated NT Sequence
tttaagaagg aganatacca tggctcacca ccaccaccac catatgggaa aggaaaaaac acatatcaac ttagtcgtta tcggccatgt cgatagtgga aaatccacga ccaccggtca tattatttac aagttgggag gtattgacag gagaaccata gaaaagtttg agaaagagtc cgccgaaatg ggtaagggta gttttaagta cgcgtgggtg ttggataagc taaaggccga aagagaaaga ggtatcacta tcgatatcgc cttatggaag ttcgaaacgc ccaggtactt cttcacagtc attgatgccc ccggtcataa ggattttatc aaaaatatga ttacgggtac gtcgcaggct gatgtggctc tcttggttgt tcctgctgag gttggtggtt ttgaaggtgc cttttcaaag gagggtcaaa ctaaggaaca cgccttgtta gccttcaccc tcggtgtgaa acagatcgtt gttggcgtca acaagatgga cactgtgaaa tactcagaag atagatacga agaaattaag aaggaagtta gggattacct gaaaaaggtc ggataccaag ctgacaaggt tgactttatc cccatttctg gatttgaagg tgataacctg attgagaagt cagacaagac cccatggtat aaaggaagga ccttgatcga ggcttcggac actatggaac cacccaagag accatacgac aaacctttaa gaattccact ccaaggtgtg tacaaaattg gtggtattgg taccgtgccc gttggcagag tcgaaactgg tattttgaag gccggtatgg ttctcaactt tgccccatct gctgtcgttt cggagtgtaa atccgtcgaa atgcacaaag aagttttgga agaggcaaga ccaggtgaca acatcggatt taacgtaaag aacgtttctg ttaaggaaat caagagagga tatgtcgctt ctgataccaa gaatgagcca gccaagggat gctccaaatt tactgcccag gttatcattt tgaaccatcc tggagaaatt aagaacggat acagtcccgt cctagattgc cacactgctc acatttcttg caaatttttg aacatcgact cgaagattga caagcgttct ggtaaggtcg ttgaggaaaa ccctaaatca attaagtctg gtgattcagc cttggttagt ctcgagccaa agaagcctat ggttgttgag accttcactg agtatccacc cctaggaaga tttgctattc gagatatgag acaaaccgtc gctgtcggta tcatcaaggc ggtcgaaaag aaggagccag gtgc
Expected Protein Sequence
MAHHHHHHMG KEKTHINLVV IGHVDSGKST TTGHIIYKLG GIDRRTIEKF EKESAEMGKG SFKYAWVLDK LKAERERGIT IDIALWKFET PRYFFTVIDA PGHKDFIKNM ITGTSQADVA LLVVPAEVGG FEGAFSKEGQ TKEHALLAFT LGVKQIVVGV NKMDTVKYSE DRYEEIKKEV RDYLKKVGYQ ADKVDFIPIS GFEGDNLIEK SDKTPWYKGR TLIEALDTME PPKRPYDKPL RIPLQGVYKI GGIGTVPVGR VETGILKAGM VLNFAPSAVV SECKSVEMHK EVLEEARPGD NIGFNVKNVS VKEIKRGYVA SDTKNEPAKG CSKFTAQVII LNHPGEIKNG YSPVLDCHTA HISCKFLNID SKIDKRSGKV VEENPKSIKS GDSALVSLEP KKPMVVETFT EYPPLGRFAI RDMRQTIAVG IIKAVEKKEP GAVSAKNPAK K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggaa aggaaaaaac acatatcaac ttagtcgtta tcggccatgt cgatagtgga aaatccacga ccaccggtca tattatttac aagttgggag gtattgacag gagaaccata gaaaagtttg agaaagagtc cgccgaaatg ggtaagggta gttttaagta cgcgtgggtg ttggataagc taaaggccga aagagaaaga ggtatcacta tcgatatcgc cttatggaag ttcgaaacgc ccaggtactt cttcacagtc attgatgccc ccggtcataa ggattttatc aaaaatatga ttacgggtac gtcgcaggct gatgtggctc tcttggttgt tcctgctgag gttggtggtt ttgaaggtgc cttttcaaag gaaggtcaaa ctaaggaaca cgccttgtta gccttcaccc tcggtgtgaa acagatcgtt gttggtgtca acaagatgga cactgtgaaa tactcagaag atagatacga agaaattaag aaggaagtta gggattacct gaaaaaggtc ggataccaag ctgacaaggt tgactttatc cccatttctg gatttgaagg tgataacctg attgagaagt cagacaagac cccatggtat aaaggaagga ccttgatcga ggctttggac actatggaac cacccaagag accatacgac aaacctttaa gaattccact ccaaggtgtg tacaaaattg gtggtattgg taccgtgccc gttggcagag tcgaaactgg tattttgaag gccggtatgg ttctcaactt tgccccatct gctgtcgttt cggagtgtaa atccgtcgaa atgcacaaag aagttttgga agaggcaaga ccaggtgaca acatcggatt taacgtaaag aacgtttctg ttaaagaaat caagagagga tatgtcgctt ctgataccaa gaatgagcca gccaagggat gctccaaatt tactgcccag gttatcattt tgaaccatcc tggagaaatt aagaacggat acagtcccgt cctagattgc cacactgctc acatttcttg caaatttttg aacatcgact cgaagattga caagcgttct ggtaaggtcg ttgaggaaaa ccctaaatca attaagtctg gtgattcagc cttggttagt ctcgagccaa agaagcctat ggttgttgag accttcactg agtatccacc cctaggaaga tttgctattc gagatatgag acaaaccatc gctgtcggta tcatcaaggc ggtcgaaaag aaggagccag gtgccgtgtc agccaaaaac ccagcaaaga aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat