SSGCID: Target Page

PlknA.18982.a

P-type ATPase, putative

CENTER ID:	PlknA.18982.a
ORGANISM:	Plasmodium knowlesi strain H
ASSOCIATED DISEASE:	Simian malaria
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlknA.18982.a.EU11.GE00397	full length		1	1294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PKH_131080
RefSeq:	XP_002260625.1
UniProt:	B3LBA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNSKSTQNDS SSTEPKEGIH NQKKKEQDGV RNSRKKATAP GVGGKKESAK GSDQAKGEDG QRARREHKPK IQAVKPKEEM EKTNVETEKT NVEMEKQIVE MAEPNVEGAK PKVETAKSKE SPKMKDEAQI PVGYNHDATE SIENLCKEFD LHDLNSGLTT EQVKINREKY GENYIEKDDA TPVWLIFLSQ YYSPVVVLLL VAALASLVLN EIVEGVAIIS IVTLNACLAT YMEKSSGDAI AKLAEMASPQ CTVLRNGNKI VIPSREVVVG DVVIISTGDS ISADLRLTEV IELKTNESLL TGESEDIKKT LTPDDCTTPF ATNLCFATTS VTNGSGKGIV TATGLNTQVG KIASQLKRSS KGSKLTPLQV ALNKLGGLIG LIAIIVLVFI ISLAVLINYR DPAHADKDPI LVIIIIGVGF AVSSVPEGLP MVVTITLSAG AKDMVRKNAN VRKLPAVETL GCCSVICSDK TGTLTEGKMT AINAVTFAKN SSLSDKNNNL TKTFDFYPTK GFEPYGGLFD SGKLTNELKK KIVIAKNQNI SYDSILYDYG NPKNDSIDVK KTRSLMFAAY LNSYDTTLAR DPKTSKWTIH GNMSEGPIVV AAAKVGYSFI TDDSHHSDVK EYTRLDDLEV TFNSSRKMKI TFYKLKKENM FEYIHLERTG KVFTHVALIK GAPDKLLDRS THLLEESPKG PQICWSAKIS ENEKDVLAQK NLELSQKALR VLAVCIKPLT SEHISLLKSL DDADERLKFV TTDEDSGFIP LGYVAAFDPP RPGVKEAIQT CRDAQVKVIM ITGDQKPTAI AIGKLIGLIG EDKAGQADQA DGHGDRANEA DAQAIECSEL HINKNPNEPV LPDDQLDAFT DKILIYSRAQ PEDKITIVQS LKRKGYLVAM TGDGVNDAPA LKAADIGVAM GINGTEVAKG ASEMILIDDN FCTVVSAIDV GRTIFSNIQK FVCFLLGTNI GEILYLSIAI AAQMPFPLEA LQILFLNLMT DGCPAVALSR EPPNHDNMKT PPRPKKQPIM TKKWWFYGII PHTVFEALCV LVSLAFSLYI CTGSYTLNDI HSSCRTVSFP NVSDESIKHE YKYFCSTYEY RVSPDYVGWI TNVNFWHPKK NKTVTFWGAA KGKVKNITPT SIEVHPEIRT IMEDGCPGDL ETDEYGWCRP KSNIYVSGKD HELPHGVFRK NFEDVASKGS KRGRTMAFIS AVWCEMLRAY TVRSWEPFYK VFNRNMWMHL ACSISATLTF LSTCIPGITS VLNTTCLLWW QYLLGISWAL LNMLLDEIVP KVIYRRRYMS VKK

NT Sequence

atgaattcca agagtacaca aaacgattca agcagcacgg agccaaagga aggaatccac aatcaaaaaa aaaaagaaca ggatggagtg aggaactcaa ggaagaaggc aactgcgcct ggtgtcggtg ggaagaagga gagtgcaaaa ggttcagacc aggcgaaggg ggaggatgga cagcgcgcaa ggcgcgagca caagccgaag atacaggccg tgaagccgaa ggaggaaatg gaaaaaacaa atgtagagac tgaaaaaaca aatgtagaaa tggaaaaaca aattgtggaa atggcagaac caaatgtgga aggggcaaaa ccgaaggtag aaacggcgaa atcgaaggag tcaccaaaaa tgaaagatga ggcgcaaatc cccgtcgggt acaaccacga cgcgaccgag agcatcgaga acctgtgcaa ggagttcgat ctccacgacc tgaattcagg tctaacgacg gagcaagtaa aaataaacag agaaaaatat ggagaaaatt acatcgaaaa ggatgatgca acaccagtat ggttgatatt cctatcccaa tattacagtc ctgtggtggt actactctta gttgctgctc tggcaagttt agttctgaat gaaattgtgg aaggagttgc catcatcagt atagttactc tgaatgcctg tctagctacg tatatggaaa agtcctcagg cgatgctatc gcaaagttag cagaaatggc atcccctcaa tgtacggttt tacgaaatgg caataaaatt gttattcctt ctagagaagt agtggtcggt gacgtagtca tcatcagtac aggagattct atatcagctg acttaagatt aacagaagtt attgaattga aaaccaatga aagtttgcta acgggagagt cagaagatat aaagaagacc ttaactccag atgattgcac cacccctttt gctaccaatt tgtgtttcgc aaccacatct gttaccaacg gttctggtaa agggatagtt actgccacgg gattaaatac ccaggtaggg aaaatcgcct ctcaattgaa aagatcaagt aagggaagta aattaactcc tctacaagta gcgttgaaca agcttggagg tcttataggt cttattgcca ttatcgtgtt ggtgtttatt atatccttgg ctgtactgat taattataga gacccggcac atgccgataa ggatcccatc cttgtgataa ttataattgg tgtgggtttt gcagtttcat ccgtcccaga aggattaccg atggttgtta caattaccct ttctgcggga gctaaagata tggttcgaaa aaatgctaat gttcgtaagt tacccgctgt ggaaacgcta ggatgctgct ctgtcatatg ttcagacaaa acaggaacgc tgacggaagg aaaaatgaca gccatcaacg ccgtaacatt tgcaaagaac tcatctttaa gtgataaaaa taataatttg actaagacat ttgattttta tcctacgaaa gggttcgaac cttatggtgg attattcgat tcaggtaaat taacaaatga attgaagaaa aaaattgtca tcgcgaagaa tcaaaacata tcttatgatt ccattttgta cgattatgga aatcccaaaa atgattccat agatgtgaag aaaaccaggt ccctcatgtt tgctgcgtac ctgaactcat acgatactac cttggcgaga gatccaaaaa cttccaaatg gaccattcat ggaaatatga gtgaaggacc catagtagtg gcagcagcga aggttgggta tagcttcatc acagacgaca gtcatcattc tgacgtcaag gagtacacaa gattggatga tttagaagtt acctttaact catccagaaa aatgaaaata actttttata aattgaagaa ggaaaacatg tttgagtata tccatttgga gagaacagga aaggtgttca cccacgttgc tctgatcaag ggtgccccag ataaactact ggatagaagc acgcaccttt tggaggaatc ccccaaggga ccgcaaatct gttggagtgc caaaattagc gaaaacgaga aggatgtatt agcccaaaaa aatctggaat tatcgcagaa ggctttgaga gttctggctg tttgcattaa accactaaca tctgaacaca tatctttgtt gaaaagttta gacgatgctg atgagcgact gaaatttgtc acgaccgatg aggatagcgg attcatcccg ttgggatacg ttgccgcgtt cgacccacca agaccaggag tgaaggaagc catccagacc tgcagagatg cccaagtgaa agttatcatg atcacagggg atcagaaacc gactgccatt gcgatcggga agctcattgg tttgataggg gaggataagg caggtcaagc ggaccaggcg gatggccatg gcgaccgggc aaacgaggca gacgcacagg ctatcgaatg ctcagagctg cacattaaca agaatccgaa tgagcccgta ttaccggatg accaattaga tgcattcaca gataaaatac tgatatactc aagagcccaa cctgaggaca aaatcaccat agtacaatcc ctgaagagga aaggctacct agtcgcaatg acaggagatg gagtcaacga cgcaccagct ttgaaagcag cagatatagg agtagccatg ggaataaacg gaacggaggt agcgaaaggt gcatctgaaa tgattctaat tgatgacaac ttctgtacag ttgttagcgc gatagatgta ggaagaacca tcttttctaa tatacaaaaa tttgtttgct tcttgttggg tacaaatatt ggagaaatac tttacctttc catagccatc gcagcacaga tgcctttccc attggaggcg cttcaaatcc tattccttaa tttaatgaca gatggatgtc cagcagtagc tctatcgagg gagccaccca accacgataa tatgaaaaca ccacccaggc caaagaagca accaataatg accaaaaaat ggtggttcta tggaatcatt cctcatacag ttttcgaagc attatgtgtc ctcgtatcat tggcattttc tctctacatt tgtacaggaa gttataccct gaatgatatc catagctctt gccgaacggt tagttttcca aatgtttcag acgaatcaat caagcatgag tacaaatact tttgcagtac ttatgaatat agagtgtcgc cagactacgt gggatggatc acgaacgtaa atttctggca tcctaagaag aataaaacag ttactttttg gggagctgca aaagggaaag taaaaaatat aactccaacg tcgatagaag tacatccaga aatacgaacc ataatggaag atggttgtcc aggtgattta gaaacagatg aatatggatg gtgtagacca aagtcaaata tttatgttag tggaaaggat catgaacttc cacatggagt ttttcgaaag aattttgaag acgttgcttc taaggggtct aagagaggta gaaccatggc atttatttca gccgtttggt gtgaaatgtt gagagcttat actgtccgca gttgggagcc tttttacaaa gtatttaata gaaatatgtg gatgcatctg gcttgtagca tttctgctac cttgactttc ttgtctacct gcattccggg aatcacttcc gtcctcaaca ccacttgttt gctctggtgg cagtacctct tgggaatttc atgggctctt cttaacatgc tgctggatga aatagtgccc aaagtcatct accgcaggag gtacatgtca gtgaagaagt aa

Details for PlknA.18982.a.EU11.GE00397

HARVESTED ON:	2/3/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	145kDa
OBSERVED MW:	145kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MGSSHHHHHH SSGENLYFQG SMNSKSTQND SSSTEPKEGI HNQKKKEQDG VRNSRKKATA PGVGGKKESA KGSDQAKGED GQRARREHKP KIQAVKPKEE MEKTNVETEK TNVEMEKQIV EMAEPNVEGA KPKVETAKSK ESPKMKDEAQ IPVGYNHDAT ESIENLCKEF DLHDLNSGLT TEQVKINREK YGENYIEKDD ATPVWLIFLS QYYSPVVVLL LVAALASLVL NEIVEGVAII SIVTLNACLA TYMEKSSGDA IAKLAEMASP QCTVLRNGNK IVIPSREVVV GDVVIISTGD SISADLRLTE VIELKTNESL LTGESEDIKK TLTPDDCTTP FATNLCFATT SVTNGSGKGI VTATGLNTQV GKIASQLKRS SKGSKLTPLQ VALNKLGGLI GLIAIIVLVF IISLAVLINY RDPAHADKDP ILVIIIIGVG FAVSSVPEGL PMVVTITLSA GAKDMVRKNA NVRKLPAVET LGCCSVICSD KTGTLTEGKM TAINAVTFAK NSSLSDKNNN LTKTFDFYPT KGFEPYGGLF DSGKLTNELK KKIVIAKNQN ISYDSILYDY GNPKNDSIDV KKTRSLMFAA YLNSYDTTLA RDPKTSKWTI HGNMSEGPIV VAAAKVGYSF ITDDSHHSDV KEYTRLDDLE VTFNSSRKMK ITFYKLKKEN MFEYIHLERT GKVFTHVALI KGAPDKLLDR STHLLEESPK GPQICWSAKI SENEKDVLAQ KNLELSQKAL RVLAVCIKPL TSEHISLLKS LDDADERLKF VTTDEDSGFI PLGYVAAFDP PRPGVKEAIQ TCRDAQVKVI MITGDQKPTA IAIGKLIGLI GEDKAGQADQ ADGHGDRANE ADAQAIECSE LHINKNPNEP VLPDDQLDAF TDKILIYSRA QPEDKITIVQ SLKRKGYLVA MTGDGVNDAP ALKAADIGVA MGINGTEVAK GASEMILIDD NFCTVVSAID VGRTIFSNIQ KFVCFLLGTN IGEILYLSIA IAAQMPFPLE ALQILFLNLM TDGCPAVALS REPPNHDNMK TPPRPKKQPI MTKKWWFYGI IPHTVFEALC VLVSLAFSLY ICTGSYTLND IHSSCRTVSF PNVSDESIKH EYKYFCSTYE YRVSPDYVGW ITNVNFWHPK KNKTVTFWGA AKGKVKNITP TSIEVHPEIR TIMEDGCPGD LETDEYGWCR PKSNIYVSGK DHELPHGVFR KNFEDVASKG SKRGRTMAFI SAVWCEMLRA YTVRSWEPFY KVFNRNMWMH LACSISATLT FLSTCIPGIT SVLNTTCLLW WQYLLGISWA LLNMLLDEIV PKVIYRRRYM SVKK

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcg agaaccttta tttccaagga tccatgaata gcaaatccac ccagaatgat tctagcagca ccgagccgaa agagggtatc cacaatcaga agaaaaaaga gcaagacggc gtgcgtaata gccgcaaaaa agctacggct ccgggcgtgg gtggtaagaa agagtccgcg aagggcagcg accaggcgaa gggtgaggac ggccaacgtg cgcgtcgtga gcacaagccg aaaattcaag ccgtgaagcc taaagaagaa atggagaaaa ccaatgtcga gactgaaaag actaatgtcg aaatggaaaa acaaatcgtc gagatggcag agccgaatgt tgagggtgca aagccgaaag tcgaaaccgc gaaatccaaa gagtccccga agatgaaaga cgaagcgcag attccggttg gctacaacca cgatgcaacc gaaagcattg agaacctgtg taaagaattc gatctgcacg acttgaattc gggtctgacg accgaacaag tcaagattaa ccgcgagaaa tatggcgaga actacatcga gaaagacgac gccaccccgg tttggctgat cttcctgagc caatactata gcccagtcgt ggttctgctg ctggtcgcag cactggcgag cctggtcttg aacgaaatcg ttgagggtgt ggctatcatc agcattgtga ccttgaatgc ctgcctggcg acgtacatgg agaaatcctc tggtgacgca atcgcgaaac tggccgaaat ggccagcccg caatgcaccg ttctgcgcaa tggcaataag atcgtgatcc cgtcccgcga ggtcgttgtt ggtgatgttg tgatcatttc gacgggcgac agcatcagcg cggatctgcg cttgactgaa gtcatcgagc tgaaaacgaa tgaaagcctg ctgacgggtg agtctgagga tatcaaaaag accctgacgc cggacgattg caccacgccg tttgctacca atctgtgttt tgctaccacg agcgtgacca acggcagcgg caagggtatt gtgaccgcaa cgggtctgaa cacccaggtc ggtaaaatcg cgtcccagct gaagcgttct agcaaaggta gcaagctgac gccgctgcaa gtcgcattaa acaaactggg tggcctgatc ggcctgatcg ctatcatcgt tcttgttttt attattagcc tggccgtgct gatcaactac cgtgatccgg cgcacgcaga taaagacccg attctggtga tcatcattat cggcgtcggt ttcgcagtta gcagcgtccc tgagggtctg cctatggttg tcacgatcac gttgagcgcg ggtgccaaag acatggttcg caaaaacgca aacgtgcgta aattgccggc agtggagact ctgggctgct gctccgttat ttgctctgac aagaccggta ccctgaccga aggtaagatg acggcaatta acgccgtaac cttcgcaaag aacagctcgc tgagcgataa gaacaataac ctgacgaaaa cctttgactt ctatccgacc aagggtttcg agccgtatgg tggcctgttt gatagcggta aactgacgaa tgaattgaag aaaaagattg tgatcgcgaa gaatcaaaac attagctacg atagcatttt gtacgattac ggcaatccga aaaacgactc tattgatgtg aagaaaactc gtagcctgat gtttgcggca tatctgaaca gctacgacac caccctggct cgtgacccga aaacctctaa gtggaccatt catggtaata tgagcgaagg tccgattgtc gttgcggcag caaaggtcgg ttacagcttc attactgatg acagccatca tagcgacgtt aaagaataca cccgcctgga cgacctggag gtgaccttta acagcagccg taaaatgaaa atcacgttct ataagctgaa aaaagaaaac atgttcgagt acattcacct ggagcgtacg ggtaaggttt tcacccatgt cgcgctgatc aaaggtgcgc cggacaagct gctggatcgt tctacccact tgctggaaga atcaccaaag ggcccacaaa tctgttggtc ggcgaagatt agcgaaaatg aaaaagatgt gctggcgcag aaaaacctgg aactgagcca gaaagccttg cgtgttctgg ccgtctgcat caagccgctg acctcggagc acattagcct gctgaaaagc ctggatgacg cggatgagcg tctgaagttc gttacgactg atgaagatag cggttttatc ccgctgggtt acgtggcggc cttcgatccg ccacgtccgg gcgtgaaaga ggccatccaa acgtgtcgtg atgcccaagt taaggttatt atgatcactg gtgaccagaa gccgacggcc atcgcgatcg gtaagctgat tggcttgatt ggcgaggaca aggctggtca agcggaccag gctgacggtc atggcgaccg cgctaacgag gctgatgcac aggccatcga gtgcagcgaa ttgcacatta acaagaatcc aaatgaaccg gtcctgccgg atgaccagct ggacgcattt acggataaga ttctgatcta ctcgcgcgca cagccggagg acaagattac cattgtacag agcctgaaac gcaagggcta cctggtagcg atgacgggtg acggcgtcaa cgatgcaccg gcgctgaaag ctgcggacat cggtgttgcg atgggcatca acggtaccga agtcgcgaag ggtgcgagcg aaatgatctt aattgatgac aacttttgta cggtcgtgag cgcgatcgac gttggccgca cgatcttctc gaatattcaa aagtttgtgt gctttctgct gggcactaac atcggtgaaa ttctgtacct gagcatcgct attgcagcgc agatgccgtt cccgctggaa gcgctgcaga ttctctttct gaacctgatg accgatggtt gcccggctgt ggcattaagc cgcgagccac cgaaccacga taacatgaaa acgccgcctc gtccgaagaa acagccgatt atgacgaaaa agtggtggtt ttatggtatc atcccacaca cggtgtttga agcgctgtgc gttctggtca gcctggcgtt cagcctgtac atttgtaccg gtagctacac cctgaatgat attcactcca gctgccgtac ggtgagcttc ccaaatgtga gcgacgagtc tatcaaacac gagtataagt acttttgctc cacctacgag tatcgtgtca gcccggatta tgtgggttgg attaccaatg tgaatttctg gcacccgaag aaaaacaaaa ccgtgacctt ctggggtgcg gcgaagggca aggtcaagaa cattaccccg accagcattg aagttcatcc agagattcgc accattatgg aagatggctg ccctggcgac ctggaaaccg atgagtacgg ctggtgtcgt ccgaaaagca atatctatgt gagcggtaaa gatcatgagt tgccgcacgg cgtctttaga aagaattttg aggacgtcgc atccaagggt tctaagcgtg gtcgtaccat ggctttcatc tctgcggttt ggtgtgagat gctgcgtgcg tataccgttc gtagctggga gccgttctac aaggttttca atcgcaacat gtggatgcat ctggcatgca gcatttccgc gaccctgacg ttcctgagca cctgtatccc tggcattacc tccgtgttga ataccacctg tctcctgtgg tggcagtatt tgctgggcat tagctgggcg ctgctgaaca tgctgctgga tgaaattgtg ccgaaagtta tctatcgtcg ccgttacatg agcgtgaaaa agtgataagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggat