SSGCID: Target Page

PlknA.18499.a

Putative uncharacterized protein

CENTER ID:	PlknA.18499.a
ORGANISM:	Plasmodium knowlesi strain H
ASSOCIATED DISEASE:	Simian malaria
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlknA.18499.a.B1.GE37945	full length		1	334

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PKH_126900
RefSeq:	XP_002260500.1
UniProt:	B3LA44

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSVSTLPYI GSKISLISNS EIRYEGILYT INTHESTVAL QNVRSFGTEG RRQPDIPPSN EIYDFIIFRG KDIKDVTVSE PAKTIPDDPA IVSMNIAPSS KNNLSDNINY NNNVNMNKPL KTQNNMMQQQ NDRNMNMNNR RYFNRQNYNF YYNNNPNNSG NNHNNNNNNN SGRNFNNFKY RNFRNYERPY IIGELESQPN PALKSKFSPD FDFSTNNLKF DKSTILDEKN KDPLTLNNNI QVGGYDKNSS FFDNISCETL DKQQGKDERV DREKLRMLDV DTFGIAAAHY RTSGHNRNNN RNRMRNNRNN KMMGNFNYNY YNRSQNPFNR YPAY

NT Sequence

atgtcttctg tatcaacctt gccatatata gggagtaaaa tttcgttaat ttcaaactcc gaaataagat atgaaggaat tttgtacacg ataaatacgc acgagtcgac cgttgcattg caaaatgtta ggtcatttgg aacggagggg agaaggcagc cagacattcc accatcaaat gaaatttatg attttataat attcagagga aaggatataa aagatgtaac cgttagtgaa cctgcgaaga ctattccaga cgacccagcg atcgtttcca tgaacatagc accatcgtct aaaaataatt tgagcgataa tataaattat aacaacaatg ttaatatgaa taagccactg aaaactcaaa acaatatgat gcaacaacaa aatgatagaa atatgaacat gaataataga cgatatttta ataggcagaa ttacaatttt tactataata ataatcctaa caatagcggt aataatcaca acaataataa taataataac agtggtagaa actttaacaa ttttaaatat agaaattttc gaaattatga aagaccgtac attatcgggg agcttgaatc acaaccaaat ccagctctaa aaagcaaatt cagccccgac tttgacttta gtacaaataa tctaaagttt gataaaagca ccatactaga cgaaaagaat aaagacccct taacgctgaa taacaatatt caagtaggag ggtatgataa gaattccagt ttttttgata atatcagttg tgaaacgtta gacaaacaac agggtaagga tgaaagagtg gaccgagaaa aattaagaat gttagatgta gacacctttg gaatagctgc agcccattat agaaccagtg gacacaatcg aaacaacaac aggaatagaa tgagaaacaa tagaaataac aaaatgatgg gaaacttcaa ctacaactat tataatagga gtcagaaccc ttttaacaga taccctgctt attag

Details for PlknA.18499.a.B1.GE37945

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/10/2014
EXPECTED MW:	40kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MAHHHHHHMS SVSTLPYIGS KISLISNSEI RYEGILYTIN THESTVALQN VRSFGTEGRR QPDIPPSNEI YDFIIFRGKD IKDVTVSEPA KTIPDDPAIV SMNIAPSSKN NLSDNINYNN NVNMNKPLKT QNNMMQQQND RNMNMNNRRY FNRQNYNFYY NNNPNNSGNN HNNNNNNNSG RNFNNFKYRN FRNYERPYII GELESQPNPA LKSKFSPDFD FSTNNLKFDK STILDEKNKD PLTLNNNIQV GGYDKNSSFF DNISCETLDK QQGKDERVDR EKLRMLDVDT FGIAAAHYRT SGHNRNNNRN RMRNNRNNK

Validated NT Sequence

gnnnnnncta gatattttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat gtcttctgta tcaaccttgc catatatagg gagtaaaatt tcgttaattt caaactccga aataagatat gaaggaattt tgtacacgat aaatacgcac gagtcgaccg ttgcattgca aaatgttagg tcatttggaa cggaggggag aaggcagcca gacattccac catcaaatga aatttatgat tttataatat tcagaggaaa ggatataaaa gatgtaaccg ttagtgaacc tgcgaagact attccagacg acccagcgat cgtttccatg aacatagcac catcgtctaa aaataatttg agcgataata taaattataa caacaatgtt aatatgaata agccactgaa aactcaaaac aatatgatgc aacaacaaaa tgatagaaat atgaacatga ataatagacg atattttaat aggcagaatt acaattttta ctataataat aatcctaaca atagcggtaa taatcacaac aataataata ataataacag tggtagaaac tttaacaatt ttaaatatag aaattttcga aattatgaaa gaccgtacat tatcggggag cttgaatcac aaccaaatcc agctctaaaa agcaaattca gccccgactt tgactttagt acaaataatc taaagtttga taaaagcacc atactagacg aaaagaataa agacccctta acgctgaata acaatattca agtaggaggg tatgataaga attccagttt ttttgataat atcagttgtg aaacgttaga caaacaacag ggtaaggatg aaagagtgga ccgagaaaaa ttaagaatgt tagatgtaga cacctttgga atagctgcag cccattatag aaccagtgga cacaatcgaa acaacaacag gaatagaatg agaaacaata gaaataacaa atgatgggaa acttcaacta cactatntaa tagngtcaga acccttttaa cngatacctg ctatagtgag tagatagatc nnnnnnnnnn nnccccnccc cnnnnnnnnn annttttnnn nannnannat nannnnnnta caannnncnn nnnnnnnnnn annnnnnnnn tnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt ctgtatcaac cttgccatat atagggagta aaatttcgtt aatttcaaac tccgaaataa gatatgaagg aattttgtac acgataaata cgcacgagtc gaccgttgca ttgcaaaatg ttaggtcatt tggaacggag gggagaaggc agccagacat tccaccatca aatgaaattt atgattttat aatattcaga ggaaaggata taaaagatgt aaccgttagt gaacctgcga agactattcc agacgaccca gcgatcgttt ccatgaacat agcaccatcg tctaaaaata atttgagcga taatataaat tataacaaca atgttaatat gaataagcca ctgaaaactc aaaacaatat gatgcaacaa caaaatgata gaaatatgaa catgaataat agacgatatt ttaataggca gaattacaat ttttactata ataataatcc taacaatagc ggtaataatc acaacaataa taataataat aacagtggta gaaactttaa caattttaaa tatagaaatt ttcgaaatta tgaaagaccg tacattatcg gggagcttga atcacaacca aatccagctc taaaaagcaa attcagcccc gactttgact ttagtacaaa taatctaaag tttgataaaa gcaccatact agacgaaaag aataaagacc ccttaacgct gaataacaat attcaagtag gagggtatga taagaattcc agtttttttg ataatatcag ttgtgaaacg ttagacaaac aacagggtaa ggatgaaaga gtggaccgag aaaaattaag aatgttagat gtagacacct ttggaatagc tgcagcccat tatagaacca gtggacacaa tcgaaacaac aacaggaata gaatgagaaa caatagaaat aacaaaatga tgggaaactt caactacaac tattataata ggagtcagaa cccttttaac agataccctg cttattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat