PlknA.17150.a

Membrane skeletal protein, putative

CENTER ID: PlknA.17150.a
ORGANISM: Plasmodium knowlesi strain H
ASSOCIATED DISEASE: Simian malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlknA.17150.a.B1.GE37940 full length 1 515
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PKH_093930
RefSeq: XP_002259421.1
UniProt: B3L5M3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQTTKSKSIS ALSSESGTTN FSKHREMERP SAAKNGADEE QFALSLKRSI SPFTTDRSYA NGQTKDTKLI DSANFDFAGK AVDTKTYITQ NKTKVIEVPE LRFIDKIEYD PFVIEKLRYV PKQVTKYNII EKPVIKNIVS EKKVDVLYVQ EKISFKDQEI VEEVYNYVDK DNNRILADQG SPSLDRSALP ITEMTQGEWD SSALSPHVNS DIRRNSIHNS NNHNDNRNSM GGASDGVLPS LLEPFGPQVN ITENKIFENV FIPKVEKVVE VKNKIDIPIN LPVPYIVPKP KIVDVDIPVF KFNDKYVPVP IRQRIIPKVT WTDKVYKVDC VVEKPYLVYH DIVKIVPTDT KISVREYPKG ITKINPEELY EVDNLALWMR VNADLKEEKD ALKKTQDNFS DHTCECSNSD SFEECSSTTE LNSYQDGATT IKSSNENVLD TLPIHPGHPL EIVHLQNKWI KQDTTRMQEL YQDGFFDAHR NAMFNLGTRI PREAEIEVRQ IAQLQRFGEK REYDN
NT Sequence
atgcaaacga cgaaaagcaa aagcatcagc gccctaagca gcgaaagtgg cacgacgaat ttctcaaagc accgcgaaat ggaacggcct tcggcggcaa aaaatggcgc agacgaagaa cagttcgccc tctcactaaa gaggagtatt tcacctttca cgactgatcg aagctacgcg aatggccaga caaaagacac aaagctgata gatagcgcaa acttcgattt cgcagggaag gcagtagaca ccaaaacgta tatcactcaa aacaaaacta aagtgataga agtccccgaa ttgagattta tagataaaat tgaatatgac ccatttgtta tcgagaagtt aaggtatgtt ccaaaacaag ttactaaata taatattatc gaaaagccag taataaaaaa cattgtatca gaaaaaaaag tggatgttct ctatgttcag gagaagataa gttttaagga ccaagaaatt gtggaagagg tttataatta cgttgataag gataacaaca ggatcttagc tgatcaagga agtccatccc ttgataggtc cgctttaccc ataacggaga tgactcaagg agaatgggac tcatcggcgt tatcccccca cgtaaatagc gacatcagac gaaatagtat ccataatagc aacaaccata atgataacag gaacagtatg gggggtgcct cagatggtgt gttgccctcc ctactggaac cattcggacc acaagtaaac attacagaaa ataagatatt cgaaaatgtg ttcataccaa aagtggaaaa agtcgtcgaa gtgaaaaata aaattgacat accgatcaac ttaccagttc cttacatagt tcctaaaccg aaaattgtag atgtagatat tcccgttttt aagtttaatg ataagtacgt gcccgtgcca attcgacaaa ggatcatacc aaaggttaca tggacagata aagtttacaa agtcgactgt gtggtggaga agccatatct ggtctatcat gacatcgtaa aaattgtgcc aactgatacg aaaatttccg ttagagagta cccaaaggga ataactaaaa taaatcccga agaattatat gaagtagata atttagcatt gtggatgagg gtaaatgctg acttgaaaga ggaaaaggac gccttgaaaa aaacccaaga caatttctct gaccatacat gtgagtgttc caattcggat tcttttgaag aatgctcttc cactacagaa ttgaattctt accaagacgg ggcaactacc atcaagtcat ctaatgaaaa tgttttggac acgctcccca tccacccggg acacccccta gagatcgttc acttgcagaa taagtggatc aaacaggaca caacgaggat gcaggaattg taccaggacg gattcttcga cgcacacaga aatgcaatgt ttaacttggg cactcgaatt ccgagagagg cagagatcga ggttaggcaa attgcgcaac tccagcggtt cggcgagaag agggagtacg acaattga
Details for PlknA.17150.a.B1.GE37940
HARVESTED ON: 4/10/2014
SEQUENCED ON: 4/8/2014
EXPECTED MW: 60kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 53
Validated AA Sequence
MAHHHHHHMQ TTKSKSISAL SSESGTTNFS KHREMERPSA AKNGADEEQF ALSLKRSISP FTTDRSYANG QTKDTKLIDS ANFDFAGKAV DTKTYITQNK TKVIEVPELR FIDKIEYDPF VIEKLRYVPK QVTKYNIIEK PVIKNIVSEK KVDVLYVQEK ISFKDQEIVE EVYNYVDKDN NRILADQGSP SLDRSALPIT EMTQGEWDSS ALSPHVNSDI RRNSIHNSNN HNDNRNSMGG ASDGVLPSLL ELFGPQVNIT ENKIFENVFI PKSGKSRRSE K
Validated NT Sequence
ttnnntnnan taattttgtt tactttaaga aggagatata ccatggctca ccaccaccac caccatatgc aaacgacgaa aagcaaaagc atcagcgccc taagcagcga aagtggcacg acgaatttct caaagcaccg cgaaatggaa cggccttcgg cggcaaaaaa tggcgcagac gaagaacagt tcgccctctc actaaagagg agtatttcac ctttcacgac tgatcgaagc tacgcgaatg gccagacaaa agacacaaag ctgatagata gcgcaaactt cgatttcgca gggaaggcag tagacaccaa aacgtatatc actcaaaaca aaactaaagt gatagaagtc cccgaattga gatttataga taaaattgaa tatgacccat ttgttatcga gaagttaagg tatgttccaa aacaagttac taaatataat attatcgaaa agccagtaat aaaaaacatt gtatcagaaa aaaaagtgga tgttctctat gttcaggaga agataagttt taaggaccaa gaaattgtgg aagaggttta taattacgtt gataaggata acaacaggat cttagctgat caaggaagtc catcccttga taggtccgct ttacccataa cggagatgac tcaaggagaa tgggactcat cggcgttatc cccccacgta aatagcgaca tcagacgaaa tagtatccat aatagcaaca accataatga taacaggaac agtatggggg gtgcctcaga tggtgtgttg ccctccctac tggaactatt cggaccacaa gtaaacatta cagaaaataa gatattcgaa aatgtgttca tacccaaaag tggaaaaagt cgtcgaagtg aaaaataaaa ttgacatacc gatcaacntt accagttcct tacatagttc ctaaaccgaa aattgtagat gtaggatatg tcccngtttt taagtttatt gattatgtat cgtgccngtg ccaatttcga caaaggatca tacccaaagg ttacatggac agataaagtt tacnantcga cttggtgtgt tggagagcat antnnngnnt atcatgaaca tcgtaaaaat tgngccactg aatacgaaan ttcnnttnnn nnnnnannna ngcgaannnn aaatnaannn cnnnaattan nntggagnna tanatttagc ntntggaatg aggnatatgn ntgnnntnnn nnnnnaaaan cnannnantt ctnnnnnnnn tannnnnnnn nntgnntnnc ac
Expected Protein Sequence
MAHHHHHHMQ TTKSKSISAL SSESGTTNFS KHREMERPSA AKNGADEEQF ALSLKRSISP FTTDRSYANG QTKDTKLIDS ANFDFAGKAV DTKTYITQNK TKVIEVPELR FIDKIEYDPF VIEKLRYVPK QVTKYNIIEK PVIKNIVSEK KVDVLYVQEK ISFKDQEIVE EVYNYVDKDN NRILADQGSP SLDRSALPIT EMTQGEWDSS ALSPHVNSDI RRNSIHNSNN HNDNRNSMGG ASDGVLPSLL EPFGPQVNIT ENKIFENVFI PKVEKVVEVK NKIDIPINLP VPYIVPKPKI VDVDIPVFKF NDKYVPVPIR QRIIPKVTWT DKVYKVDCVV EKPYLVYHDI VKIVPTDTKI SVREYPKGIT KINPEELYEV DNLALWMRVN ADLKEEKDAL KKTQDNFSDH TCECSNSDSF EECSSTTELN SYQDGATTIK SSNENVLDTL PIHPGHPLEI VHLQNKWIKQ DTTRMQELYQ DGFFDAHRNA MFNLGTRIPR EAEIEVRQIA QLQRFGEKRE YDN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa cgacgaaaag caaaagcatc agcgccctaa gcagcgaaag tggcacgacg aatttctcaa agcaccgcga aatggaacgg ccttcggcgg caaaaaatgg cgcagacgaa gaacagttcg ccctctcact aaagaggagt atttcacctt tcacgactga tcgaagctac gcgaatggcc agacaaaaga cacaaagctg atagatagcg caaacttcga tttcgcaggg aaggcagtag acaccaaaac gtatatcact caaaacaaaa ctaaagtgat agaagtcccc gaattgagat ttatagataa aattgaatat gacccatttg ttatcgagaa gttaaggtat gttccaaaac aagttactaa atataatatt atcgaaaagc cagtaataaa aaacattgta tcagaaaaaa aagtggatgt tctctatgtt caggagaaga taagttttaa ggaccaagaa attgtggaag aggtttataa ttacgttgat aaggataaca acaggatctt agctgatcaa ggaagtccat cccttgatag gtccgcttta cccataacgg agatgactca aggagaatgg gactcatcgg cgttatcccc ccacgtaaat agcgacatca gacgaaatag tatccataat agcaacaacc ataatgataa caggaacagt atggggggtg cctcagatgg tgtgttgccc tccctactgg aaccattcgg accacaagta aacattacag aaaataagat attcgaaaat gtgttcatac caaaagtgga aaaagtcgtc gaagtgaaaa ataaaattga cataccgatc aacttaccag ttccttacat agttcctaaa ccgaaaattg tagatgtaga tattcccgtt tttaagttta atgataagta cgtgcccgtg ccaattcgac aaaggatcat accaaaggtt acatggacag ataaagttta caaagtcgac tgtgtggtgg agaagccata tctggtctat catgacatcg taaaaattgt gccaactgat acgaaaattt ccgttagaga gtacccaaag ggaataacta aaataaatcc cgaagaatta tatgaagtag ataatttagc attgtggatg agggtaaatg ctgacttgaa agaggaaaag gacgccttga aaaaaaccca agacaatttc tctgaccata catgtgagtg ttccaattcg gattcttttg aagaatgctc ttccactaca gaattgaatt cttaccaaga cggggcaact accatcaagt catctaatga aaatgttttg gacacgctcc ccatccaccc gggacacccc ctagagatcg ttcacttgca gaataagtgg atcaaacagg acacaacgag gatgcaggaa ttgtaccagg acggattctt cgacgcacac agaaatgcaa tgtttaactt gggcactcga attccgagag aggcagagat cgaggttagg caaattgcgc aactccagcg gttcggcgag aagagggagt acgacaattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat