SSGCID: Target Page

PlknA.01238.a

Seryl-trna synthetase, putative

CENTER ID:	PlknA.01238.a
ORGANISM:	Plasmodium knowlesi strain H
ASSOCIATED DISEASE:	Simian malaria
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlknA.01238.a.B1.GE37776	full length		1	554

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PKH_143310
RefSeq:	XP_002262235.1
UniProt:	B3LCF4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHMRNLSLLC ILLCVHWALG RRNRHGIKYY INPCYGDPQW RDLRAVKKRT PLVCKNKCTG DQWREDEQTE CGMSLNILKE NENKVIENLK KRGMDKNIDN VKILKDLIEE KNKLEVLRNN LRNMRKVLSD RVRNLMFAEN ATDESATQGR MSDETPLYID RSKVETEGHN PLEDTPFEEY NVERVKEQIN QINEQININE TKITHLRYKI EDHFSRLPNL LLNKVPEGKK NNKNKIIKMY KRKNIQTDKD NTSIEPHEEI LKRFDRNQHI FANITNKIGF GYNILVNNIA KLERALINFM INTHVNKFHY TYVKTPVIVA RSALTNTGQL PKFENDLFKI NDNYKILNEE AFLIPTSEVS LLNLFRNAHL NFENLPIRLV SHSSCFRIEK NFTYGKTSKG LLREHIFEKV ELVNITDRRS SHVFYKDLIK HSEYILKKLK IPYRVVLLNS FETPFSASIC YDLEAWLPSQ QRFIEVSSCS NCLDFQARKL NLKYKKKDSN FFCHTINGSG LAVGRVLAII LEQYQIGGNS PNEKKELIIP KPLRKFMNAS IISL

NT Sequence

atgcatatga gaaacctttc cctactctgc atactgctat gcgtacattg ggcacttgga aggcggaaca gacatgggat caagtactac ataaacccat gttacgggga cccccaatgg agagacctac gggcagtgaa aaaacgcacc cccttagtgt gcaagaacaa atgcactggt gaccaatgga gagaagacga acaaaccgaa tgtggtatga gtcttaacat tttaaaagaa aatgaaaata aggtaattga aaatttaaaa aaaaggggaa tggataaaaa tattgacaat gttaaaatct taaaagattt aatagaggaa aaaaataaac tcgaagtgtt aagaaataat ctccgaaata tgagaaaagt tctatccgat cgggtgagaa atttaatgtt tgctgaaaat gccactgatg aaagtgcaac tcaggggaga atgtcagacg aaacaccact ctacattgat aggagtaaag tggaaacgga aggacacaat ccactagagg ataccccttt tgaagaatac aatgtcgaac gagtgaaaga acaaattaac cagataaacg agcagattaa tattaacgaa acgaagataa cccatttgag atacaaaata gaggaccatt tcagtagact ccccaacctt ctactgaaca aagtaccgga ggggaaaaaa aacaacaaga acaaaataat caaaatgtat aaaaggaaga acatacaaac ggacaaagat aacacctcca tagaaccgca tgaagaaatt ttaaaaagat tcgacaggaa tcaacacatt tttgcaaaca taacaaacaa aattggcttt ggctataaca tactagtaaa taatattgca aaattggaaa gggcattaat taacttcatg attaatacac atgtaaataa atttcattac acgtatgtga aaacgccagt aatagttgcc agatcggcct taacaaacac tggacaatta ccaaaattcg aaaacgattt atttaaaatt aatgacaatt ataaaattct taatgaagaa gcttttctaa ttcctactag cgaagtttct ctattaaacc tatttagaaa tgcccaccta aattttgaaa atttacctat tagattagtc agccattctt catgttttag gatagaaaaa aactttacct atggaaaaac atccaaggga ttgttgcgtg aacacatttt cgaaaaagta gaattagtta acatcaccga taggaggagt tctcatgtgt tttacaaaga tttaattaaa catagtgaat atattttaaa gaaattaaaa attccataca gagtagttct tctaaattcc tttgaaactc cattttctgc atccatctgt tatgatttag aagcctggtt accaagccag caacgattta ttgaagtttc ttcctgttcc aattgcttag atttccaggc aagaaaacta aatttaaaat ataaaaaaaa ggacagtaac tttttctgcc atactattaa tggatccggt ttggccgtag ggcgtgtctt ggcaatcatc ctagaacagt accaaatagg ggggaactcc ccaaatgaaa aaaaggagct cataattccg aagcccttgc ggaagtttat gaacgcgtca atcattagtt tgtag

Details for PlknA.01238.a.B1.GE37776

HARVESTED ON:	2/6/2014
SEQUENCED ON:	data unavailable
EXPECTED MW:	66kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	failed sequencing
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMH MRNLSLLCIL LCVHWALGRR NRHGIKYYIN PCYGDPQWRD LRAVKKRTPL VCKNKCTGDQ WREDEQTECG MSLNILKENE NKVIENLKKR GMDKNIDNVK ILKDLIEEKN KLEVLRNNLR NMRKVLSDRV RNLMFAENAT DESATQGRMS DETPLYIDRS KVETEGHNPL EDTPFEEYNV ERVKEQINQI NEQININETK ITHLRYKIED HFSRLPNLLL NKVPEGKKNN KNKIIKMYKR KNIQTDKDNT SIEPHEEILK RFDRNQHIFA NITNKIGFGY NILVNNIAKL ERALINFMIN THVNKFHYTY VKTPVIVARS ALTNTGQLPK FENDLFKIND NYKILNEEAF LIPTSEVSLL NLFRNAHLNF ENLPIRLVSH SSCFRIEKNF TYGKTSKGLL REHIFEKVEL VNITDRRSSH VFYKDLIKHS EYILKKLKIP YRVVLLNSFE TPFSASICYD LEAWLPSQQR FIEVSSCSNC LDFQARKLNL KYKKKDSNFF CHTINGSGLA VGRVLAIILE QYQIGGNSPN EKKELIIPKP LRKFMNASII SL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata tgagaaacct ttccctactc tgcatactgc tatgcgtaca ttgggcactt ggaaggcgga acagacatgg gatcaagtac tacataaacc catgttacgg ggacccccaa tggagagacc tacgggcagt gaaaaaacgc acccccttag tgtgcaagaa caaatgcact ggtgaccaat ggagagaaga cgaacaaacc gaatgtggta tgagtcttaa cattttaaaa gaaaatgaaa ataaggtaat tgaaaattta aaaaaaaggg gaatggataa aaatattgac aatgttaaaa tcttaaaaga tttaatagag gaaaaaaata aactcgaagt gttaagaaat aatctccgaa atatgagaaa agttctatcc gatcgggtga gaaatttaat gtttgctgaa aatgccactg atgaaagtgc aactcagggg agaatgtcag acgaaacacc actctacatt gataggagta aagtggaaac ggaaggacac aatccactag aggatacccc ttttgaagaa tacaatgtcg aacgagtgaa agaacaaatt aaccagataa acgagcagat taatattaac gaaacgaaga taacccattt gagatacaaa atagaggacc atttcagtag actccccaac cttctactga acaaagtacc ggaggggaaa aaaaacaaca agaacaaaat aatcaaaatg tataaaagga agaacataca aacggacaaa gataacacct ccatagaacc gcatgaagaa attttaaaaa gattcgacag gaatcaacac atttttgcaa acataacaaa caaaattggc tttggctata acatactagt aaataatatt gcaaaattgg aaagggcatt aattaacttc atgattaata cacatgtaaa taaatttcat tacacgtatg tgaaaacgcc agtaatagtt gccagatcgg ccttaacaaa cactggacaa ttaccaaaat tcgaaaacga tttatttaaa attaatgaca attataaaat tcttaatgaa gaagcttttc taattcctac tagcgaagtt tctctattaa acctatttag aaatgcccac ctaaattttg aaaatttacc tattagatta gtcagccatt cttcatgttt taggatagaa aaaaacttta cctatggaaa aacatccaag ggattgttgc gtgaacacat tttcgaaaaa gtagaattag ttaacatcac cgataggagg agttctcatg tgttttacaa agatttaatt aaacatagtg aatatatttt aaagaaatta aaaattccat acagagtagt tcttctaaat tcctttgaaa ctccattttc tgcatccatc tgttatgatt tagaagcctg gttaccaagc cagcaacgat ttattgaagt ttcttcctgt tccaattgct tagatttcca ggcaagaaaa ctaaatttaa aatataaaaa aaaggacagt aactttttct gccatactat taatggatcc ggtttggccg tagggcgtgt cttggcaatc atcctagaac agtaccaaat aggggggaac tccccaaatg aaaaaaagga gctcataatt ccgaagccct tgcggaagtt tatgaacgcg tcaatcatta gtttgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat