PlknA.00879.a

Actin, putative

CENTER ID: PlknA.00879.a
ORGANISM: Plasmodium knowlesi strain H
ASSOCIATED DISEASE: Simian malaria
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlknA.00879.a.B1.GE37935 full length 1 379
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PKH_020950
RefSeq: XP_002257769.1
UniProt: B3KZN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNEYAKQLYS NQPIIIDNGS GYIKAGFAGD DVPNLVFPSY VGRPKYKRVM AGALEGNIFV GNKAEEYRGL LKVTYPINHG IIENWNDMEN IWIHVYNSMK INSEEHPVLL TEAPLNPQKN KEKIAEVFFE SFNVPALFIS IQAILSLYSC GKTNGTVLDC GDGVCHCVSI YEGYSITNTI TRTDVAGRDI TTYLGYLLRK NGHLFNTSAE MEVVKNMKEN CCYVSFNMSK EKNSSEKALT TLPYILPDGS QILIGSERYR APEVLFNPSI LGLEYLGLSE LIVTSITRAD MDLRRTLYSH IVLSGGTTMF HGFGDRLLNE IRKFAPKDIT IRISAPPERK FSTFIGGSIL ASLATFKKIW ISKQEFDEYG SVILHRKTF
NT Sequence
atgaacgagt atgccaagca actttattca aatcagccta ttataataga caatgggagt gggtacataa aagctgggtt cgcaggggat gatgtgccca accttgtatt tccttcctac gtggggaggc ccaagtacaa gagggtgatg gccggagcgc tggagggaaa catctttgtg gggaacaagg cggaggagta cagaggtctt ctgaaggtga cctaccccat caaccacggc attattgaga attggaatga catggaaaat atttggatcc acgtttacaa ctcgatgaag ataaattcgg aggagcaccc cgtattattg acagaggccc cactgaaccc ccagaagaat aaagaaaaaa tcgcggaagt ctttttcgaa tcttttaatg tgcctgcgct gttcatctcc atccaggcca ttttatcttt gtactcctgt gggaagacaa acggcacggt gctcgattgc ggggacggag tatgtcactg cgtgtccatt tacgagggct atagcataac caacacgata acccggacgg atgtcgcggg ccgagatatc acgacctacc tgggctatct gttaaggaaa aatggccacc tcttcaatac ctcagccgaa atggaagtcg taaaaaatat gaaggagaac tgctgctatg tctcctttaa catgagcaag gagaaaaact catccgagaa ggccctcacg actttgccgt atatccttcc tgatgggtcg caaatactga tcggatccga gaggtacagg gccccggagg ttctgttcaa cccctccatt cttgggctgg aatatctggg attgtctgaa cttatcgtta cgtccataac acgagcggac atggatctac gacggacact atactcccac atcgtgttgt ctggcggcac gaccatgttt cacggatttg gcgatcggtt actaaacgaa ataaggaaat tcgccccaaa ggatattacg attcgcatta gcgccccgcc agagcgcaag ttcagcacat tcatcggggg gtctattctg gcctctttgg caacctttaa gaagatatgg atttcaaagc aggaatttga cgaatacgga tctgtgatac tccacaggaa gaccttttga
Details for PlknA.00879.a.B1.GE37935
HARVESTED ON: 4/10/2014
SEQUENCED ON: 4/8/2014
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 34
PERCENT COVERAGE: 33
Validated AA Sequence
MAHHHHHMNE YAKQIEDLFQ KVRSRGNCES EQVTGDKEKK KKKKKKFWGG APPLFFFANL FPQRENPFSQ ISLFRGGGPP LPQTRIALFG VKPPGGKKGK NPPAPPKKEK KKKKKKTFSP PLGGXPFTFF
Validated NT Sequence
cannncnnnn nnnnnnncnn nnnnatnatt ttgtttaact ttaagaagga gatataccat ggctcaccac caccaccata tgaacgagta tgccaagcaa atagaagacc tcttccaaaa ggtgagaagc aggggcaact gcgaaagtga acaagtgact ggggataagg aaaaaaaaaa aaaaaaaaaa aaaaaatttt gggggggggc cccccccctt tttttttttg caaacctttt cccccaaagg gaaaaccctt tttcccaaat ttcccttttt cggggggggg gccccccact cccccaaacc cgaattgccc tttttggggt aaaaccccct gggggaaaaa aaggcaaaaa tcccccggcc ccccccaaaa aggaaaaaaa aaaaaaaaaa aaaaaaacct tttccccccc ccttgggggg ncccctttca cttttttttg aaancccaaa aaaaaaaaaa ggntttttcc ggggggnngn ctntntaaaa aaaaagggaa aaaaaacccc tttnggggaa nccccnaact nnnnncccnn aaaaanngga aaaatnnaaa nanttntttt ttnaagggtt tccccnncaa annaanaaaa aaattttttt tttttttttt ccgttgtnan acnncnnggg gnaanttttg aaaaaaaaat tnaaannaaa ttttttnnaa anaanancnc nntnnnnngg ttttnnnaaa atttgggggn ncnnnttnct gnna
Expected Protein Sequence
MAHHHHHHMN EYAKQLYSNQ PIIIDNGSGY IKAGFAGDDV PNLVFPSYVG RPKYKRVMAG ALEGNIFVGN KAEEYRGLLK VTYPINHGII ENWNDMENIW IHVYNSMKIN SEEHPVLLTE APLNPQKNKE KIAEVFFESF NVPALFISIQ AILSLYSCGK TNGTVLDCGD GVCHCVSIYE GYSITNTITR TDVAGRDITT YLGYLLRKNG HLFNTSAEME VVKNMKENCC YVSFNMSKEK NSSEKALTTL PYILPDGSQI LIGSERYRAP EVLFNPSILG LEYLGLSELI VTSITRADMD LRRTLYSHIV LSGGTTMFHG FGDRLLNEIR KFAPKDITIR ISAPPERKFS TFIGGSILAS LATFKKIWIS KQEFDEYGSV ILHRKTF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg agtatgccaa gcaactttat tcaaatcagc ctattataat agacaatggg agtgggtaca taaaagctgg gttcgcaggg gatgatgtgc ccaaccttgt atttccttcc tacgtgggga ggcccaagta caagagggtg atggccggag cgctggaggg aaacatcttt gtggggaaca aggcggagga gtacagaggt cttctgaagg tgacctaccc catcaaccac ggcattattg agaattggaa tgacatggaa aatatttgga tccacgttta caactcgatg aagataaatt cggaggagca ccccgtatta ttgacagagg ccccactgaa cccccagaag aataaagaaa aaatcgcgga agtctttttc gaatctttta atgtgcctgc gctgttcatc tccatccagg ccattttatc tttgtactcc tgtgggaaga caaacggcac ggtgctcgat tgcggggacg gagtatgtca ctgcgtgtcc atttacgagg gctatagcat aaccaacacg ataacccgga cggatgtcgc gggccgagat atcacgacct acctgggcta tctgttaagg aaaaatggcc acctcttcaa tacctcagcc gaaatggaag tcgtaaaaaa tatgaaggag aactgctgct atgtctcctt taacatgagc aaggagaaaa actcatccga gaaggccctc acgactttgc cgtatatcct tcctgatggg tcgcaaatac tgatcggatc cgagaggtac agggccccgg aggttctgtt caacccctcc attcttgggc tggaatatct gggattgtct gaacttatcg ttacgtccat aacacgagcg gacatggatc tacgacggac actatactcc cacatcgtgt tgtctggcgg cacgaccatg tttcacggat ttggcgatcg gttactaaac gaaataagga aattcgcccc aaaggatatt acgattcgca ttagcgcccc gccagagcgc aagttcagca cattcatcgg ggggtctatt ctggcctctt tggcaacctt taagaagata tggatttcaa agcaggaatt tgacgaatac ggatctgtga tactccacag gaagaccttt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat