SSGCID: Target Page

PlknA.00188.a

Casein kinase ii, alpha subunit, putative

CENTER ID:	PlknA.00188.a
ORGANISM:	Plasmodium knowlesi strain H
ASSOCIATED DISEASE:	Simian malaria
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlknA.00188.a.B1.GE37338	full length		1	335

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PKH_090560
RefSeq:	XP_002259084.1
UniProt:	B3L4N6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSSSISKRI YIPKYYADVN IHKPKEYYDY DNLELQWNKP NRYEIMKKIG RGKYSEVFNG YDTEYNRPCA IKVLKPVKKK KIKREIKILQ NLHGGPNIIK LLDIVKDPVT KTPSLIFEYI NNIDFKTLYP KFTDKDIRYY IYQILKALDY CHSQGIMHRD VKPHNIMIDH ENKQIRLIDW GLAEFYHPGQ EYNVRVASRY YKGPELLIDL QLYDYSLDIW SLGCMLAGMI FKKEPFFCGH DNYDQLVKIA KVLGTDDLHA YLKKYNIKLK PHYLNILGEY ERKPWSHFLT QSNIDIAKDE VIDLIDKMLI YDHAKRIAPK EAMEHPYFSE VREES

NT Sequence

atgtcttcga gctccatcag caaaaggatt tacattccca agtactacgc tgatgtgaat attcataagc cgaaagaata ttatgattac gacaatttgg agctacaatg gaataagcca aatcggtacg aaattatgaa gaagattggg aggggaaaat acagcgaagt gtttaatggg tatgacacgg agtacaacag accttgtgca ataaaagtat taaaaccagt gaagaaaaaa aaaataaaga gagaaataaa aatcttgcaa aacttacatg gaggaccaaa cattatcaag ctgttagaca tagtcaaaga tccagttaca aaaactcctt cactcatttt tgaatatatt aataacatcg attttaaaac gttatatcca aaatttacgg acaaagatat acgctattat atttaccaaa ttcttaaggc tttagattat tgccatagcc agggcattat gcatagggat gtgaagccac acaacattat gattgatcat gagaacaagc aaattcgact aatagattgg ggtttagctg agttttacca ccctggacaa gaatacaatg taagagttgc aagcaggtat tacaaaggac cagaacttct catcgacttg cagctctacg attactctct agacatttgg agtttagggt gtatgcttgc tggaatgata tttaagaagg aacctttttt ttgtggacat gataattatg atcagcttgt caaaattgct aaagtcttag gcactgatga tcttcatgcc tacttaaaaa aatacaacat taagttgaag ccccattatt taaatatact gggtgagtat gagaggaaac catggtccca tttcctcact caatccaaca tcgacatcgc caaggatgaa gtcattgacc ttattgataa gatgctaatt tacgaccacg cgaagaggat tgccccgaag gaagccatgg agcatcccta ctttagcgag gttcgtgagg agtcgtga

Details for PlknA.00188.a.B1.GE37338

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	82

Validated AA Sequence

MKKIGRGKYS EVFNGYDTEY NRPCAIKVLK PVKKKKIKRE IKILQNLHGG PNIIKLLDIV KDPVTKTPSL IFEYINNIDF KTLYPKFTDK DIRYYIYQIL KALDYCHSQG IMHRDVKPHN IMIDHENKQI RLIDWGLAEF YHPGQEYNVR VASRYYKGPE LLIDLQLYDY SLDIWSLGCM LAGMIFKKEP FFCGHDNYDQ LVKIAKVLGT DDLHAYLKKY NIKLKPHYLN ILGEYERKPW SHFLTQSNID IAKDEVIDLI DKMLIYDHAK RIAPKEAMEH PYF

Validated NT Sequence

taaagtaggg atgctccatg gcttccttcg gggcaatcct cttcgcgtgg tcgtaaatta gcatcttatc aataaggtca atgacttcat ccttggcgat gtcgatgttg gattgagtga ggaaatggga ccatggtttc ctctcatact cacccagtat atttaaataa tggggcttca acttaatgtt gtattttttt aagtaggcat gaagatcatc agtgcctaag actttagcaa ttttgacaag ctgatcataa ttatcatgtc cacaaaaaaa aggttccttc ttaaatatca ttccagcaag catacaccct aaactccaaa tgtctagaga gtaatcgtag agctgcaagt cgatgagaag ttctggtcct ttgtaatacc tgcttgcaac tcttacattg tattcttgtc cagggtggta aaactcagct aaaccccaat ctattagtcg aatttgcttg ttctcatgat caatcataat gttgtgtggc ttcacatccc tatgcataat gccctggcta tggcaataat ctaaagcctt aagaatttgg taaatataat agcgtatatc tttgtccgta aattttggat ataacgtttt aaaatcgatg ttattaatat attcaaaaat gagtgaagga gtttttgtaa ctggatcttt gactatgtct aacagcttga taatgtttgg tcctccatgt aagttttgca agatttttat ttctctcttt attttttttt tcttcactgg ttttaatact tttattgcac aaggtctgtt gtactccgtg tcatacccat taaacacttc gctgtatttt cccctcccaa tcttcttcat aatttcgtac cgatttggct tattccattg tagctccaaa ttgtcgtaat cataatattc tttcggctta tgaatattca catcagcgta gtacttggga atgtaaatcc ttttgctgat ggagctcga

Expected Protein Sequence

MAHHHHHHMS SSSISKRIYI PKYYADVNIH KPKEYYDYDN LELQWNKPNR YEIMKKIGRG KYSEVFNGYD TEYNRPCAIK VLKPVKKKKI KREIKILQNL HGGPNIIKLL DIVKDPVTKT PSLIFEYINN IDFKTLYPKF TDKDIRYYIY QILKALDYCH SQGIMHRDVK PHNIMIDHEN KQIRLIDWGL AEFYHPGQEY NVRVASRYYK GPELLIDLQL YDYSLDIWSL GCMLAGMIFK KEPFFCGHDN YDQLVKIAKV LGTDDLHAYL KKYNIKLKPH YLNILGEYER KPWSHFLTQS NIDIAKDEVI DLIDKMLIYD HAKRIAPKEA MEHPYFSEVR EES

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt cgagctccat cagcaaaagg atttacattc ccaagtacta cgctgatgtg aatattcata agccgaaaga atattatgat tacgacaatt tggagctaca atggaataag ccaaatcggt acgaaattat gaagaagatt gggaggggaa aatacagcga agtgtttaat gggtatgaca cggagtacaa cagaccttgt gcaataaaag tattaaaacc agtgaagaaa aaaaaaataa agagagaaat aaaaatcttg caaaacttac atggaggacc aaacattatc aagctgttag acatagtcaa agatccagtt acaaaaactc cttcactcat ttttgaatat attaataaca tcgattttaa aacgttatat ccaaaattta cggacaaaga tatacgctat tatatttacc aaattcttaa ggctttagat tattgccata gccagggcat tatgcatagg gatgtgaagc cacacaacat tatgattgat catgagaaca agcaaattcg actaatagat tggggtttag ctgagtttta ccaccctgga caagaataca atgtaagagt tgcaagcagg tattacaaag gaccagaact tctcatcgac ttgcagctct acgattactc tctagacatt tggagtttag ggtgtatgct tgctggaatg atatttaaga aggaaccttt tttttgtgga catgataatt atgatcagct tgtcaaaatt gctaaagtct taggcactga tgatcttcat gcctacttaa aaaaatacaa cattaagttg aagccccatt atttaaatat actgggtgag tatgagagga aaccatggtc ccatttcctc actcaatcca acatcgacat cgccaaggat gaagtcattg accttattga taagatgcta atttacgacc acgcgaagag gattgccccg aaggaagcca tggagcatcc ctactttagc gaggttcgtg aggagtcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat