SSGCID: Target Page

PlknA.00179.a

Orotate phosphoribosyltransferase, putative (EC 2.4.2.10)

CENTER ID:	PlknA.00179.a
ORGANISM:	Plasmodium knowlesi strain H
ASSOCIATED DISEASE:	Simian malaria
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlknA.00179.a.B1.GE37337	full length		1	254

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PKH_101980
RefSeq:	XP_002259686.1
UniProt:	B3L6D8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEEEKSTKQT NYLSDEDLHK KYIHLRECIE LEKDDMDNCH VKEMKKLLIT ALLKYKAIKF GDFILKSKRK SKYFFSSGVL NNIVSAHIIS FLISHLILKE KIPFDYLLGA SYKGIPIATL TSHFLFQSNK FSNVFYLYDR KEKKDYGDKT LIVGNLDEEF NGDVHNAKDE KNEKKVIIID DVFTCGTALT EIMNKLKSYP NLKVVAFIVL LNRNEYELNE HNEKVYFKDL FEQKLKVPLY SILSYNEDLE PLMG

NT Sequence

atggaagaag agaaaagtac caaacaaacg aactatctca gtgatgaaga tttacacaaa aaatatatcc atcttcgaga atgcatcgaa ctggagaagg atgacatgga caactgccac gtgaaggaaa tgaaaaaatt gctcatcaca gcattgttaa aatataaggc cataaaattt ggtgatttta ttttaaaatc aaagagaaaa tcaaaatatt ttttttcaag tggagtatta aataacatcg tttccgcaca cattatttcg tttttaattt ctcatttaat tttaaaggaa aaaattccct ttgattattt attgggggca tcttataagg gcattccaat agcaaccctg actagccact ttttatttca gtcgaataaa ttttcaaatg ttttttacct gtacgatagg aaggaaaaaa aagattatgg tgacaagaca ctcattgttg ggaacttaga tgaagaattt aatggcgatg tacacaatgc aaaggatgaa aagaacgaaa aaaaagtaat tataattgat gatgtcttta cctgtggcac tgccttgacg gaaataatga acaagctaaa gtcatatccc aacttgaagg ttgttgcttt catcgtttta cttaatcgaa atgagtacga actgaatgag cacaatgaaa aggtgtattt caaagatttg tttgaacaga aactcaaggt ccctctttac agcattctca gttacaacga ggacctggag cccctcatgg gttag

Details for PlknA.00179.a.B1.GE37337

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	31kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME EEKSTKQTNY LSDEDLHKKY IHLRECIELE KDDMDNCHVK EMKKLLITAL LKYKAIKFGD FILKSKRKSK YFFSSGVLNN IVSAHIISFL ISHLILKEKI PFDYLLGASY KGIPIATLTS HFLFQSNKFS NVFYLYDRKE KKDYGDKTLI VGNLDEEFNG DVHNAKDEKN EKKVIIIDDV FTCGTALTEI MNKLKSYPNL KVVAFIVLLN RNEYELNEHN EKVYFKDLFE QKLKVPLYSI LSYNEDLEPL MG

Validated NT Sequence

tcctatctta ctcncttaac ccatgagggg ctccaggtcc tcgttgtaac tgagaatgct gtaaagaggg accttgagtt tctgttcaaa caaatctttg aaatacacct tttcattgtg ctcattcagt tcgtactcat ttcgattaag taaaacgatg aaagcaacaa ccttcaagtt gggatatgac tttagcttgt tcattatttc cgtcaaggca gtgccacagg taaagacatc atcaattata attacttttt tttcgttctt ttcatccttt gcattgtgta catcgccatt aaattcttca tctaagttcc caacaatgag tgtcttgtca ccataatctt ttttttcctt cctatcgtac aggtaaaaaa catttgaaaa tttattcgac tgaaataaaa agtggctagt cagggttgct attggaatgc ccttataaga tgcccccaat aaataatcaa agggaatttt ttcctttaaa attaaatgag aaattaaaaa cgaaataatg tgtgcggaaa cgatgttatt taatactcca cttgaaaaaa aatattttga ttttctcttt gattttaaaa taaaatcacc aaattttatg gccttatatt ttaacaatgc tgtgatgagc aattttttca tttccttcac gtggcagttg tccatgtcat ccttctccag ttcgatgcat tctcgaagat ggatatattt tttgtgtaaa tcttcatcac tgagatagtt cgtttgtttg gtacttttct cttcttccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag aagagaaaag taccaaacaa acgaactatc tcagtgatga agatttacac aaaaaatata tccatcttcg agaatgcatc gaactggaga aggatgacat ggacaactgc cacgtgaagg aaatgaaaaa attgctcatc acagcattgt taaaatataa ggccataaaa tttggtgatt ttattttaaa atcaaagaga aaatcaaaat attttttttc aagtggagta ttaaataaca tcgtttccgc acacattatt tcgtttttaa tttctcattt aattttaaag gaaaaaattc cctttgatta tttattgggg gcatcttata agggcattcc aatagcaacc ctgactagcc actttttatt tcagtcgaat aaattttcaa atgtttttta cctgtacgat aggaaggaaa aaaaagatta tggtgacaag acactcattg ttgggaactt agatgaagaa tttaatggcg atgtacacaa tgcaaaggat gaaaagaacg aaaaaaaagt aattataatt gatgatgtct ttacctgtgg cactgccttg acggaaataa tgaacaagct aaagtcatat cccaacttga aggttgttgc tttcatcgtt ttacttaatc gaaatgagta cgaactgaat gagcacaatg aaaaggtgta tttcaaagat ttgtttgaac agaaactcaa ggtccctctt tacagcattc tcagttacaa cgaggacctg gagcccctca tgggttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat