SSGCID: Target Page

PlknA.00043.a

Dihydroorotase, putative (EC 3.5.2.3)

CENTER ID:	PlknA.00043.a
ORGANISM:	Plasmodium knowlesi strain H
ASSOCIATED DISEASE:	Simian malaria
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlknA.00043.a.B1.GE37336	full length		1	361

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlknA.00043.a.B1.PW37388	full length		1	361

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PKH_123240
RefSeq:	XP_002260134.1
UniProt:	B3L928

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEPLQIPLGD DMHCHLRQGD MLSFTVGAIK KGGCDRVLVM PNTTPIISTC EEAKKYRSEL IKQDPSVDYL MTLYLNRKTD SNDILRNHEE CNLQGVKIYP SNVTTNSNHG VTTLEAYYEI FHTLEKLNKS LHIHCEEPNV NPLYAERSYV QHIHDLAVHF PHLKIVLEHI STVEMINLVR TYPNIAGSVT PHHLQLTIDD VVDTESYDYA PNVVTIEKYI KNVYNYCKPL PKTIDDKVAL CKIIQEGFPR IFLGSDSAPH YREHKNDPHY KPGIYTQPFL MSYVSHIFNK INSLDKVENF ACKNAAIFLN LKEKKTDTNE QSLSLRIQKK GLKIPDEYFG VVPFLAGQTI DFTASFVGAD V

NT Sequence

atggaaccgc tgcagattcc cctgggagac gacatgcact gccacctgag gcagggtgac atgctgagct tcaccgttgg ggcaatcaag aagggggggt gcgaccgcgt gctcgtaatg cccaacacca caccaatcat aagcacctgt gaagaagcca aaaaatatag aagcgagcta atcaagcagg accccagcgt ggactaccta atgacgttat acttgaacag aaaaacagat tcaaatgaca tcctcagaaa ccacgaagaa tgcaatttgc aaggagttaa aatatacccc agcaatgtaa caaccaactc taatcatgga gttacaacgt tagaagcata ttatgaaatt ttccatacac ttgaaaagtt aaacaaaagt ttgcacatac attgtgaaga acccaacgtg aatccacttt atgcagaaag gagttacgta cagcatatac atgacttggc tgtccacttc ccccatttga aaatcgtact agaacacatt tctacggtag aaatgataaa cttggttagg acatacccga acatcgcagg ttcagttact ccgcaccatt tgcaactaac catagacgat gttgtagaca cggagagcta cgactatgct cctaacgtgg taaccataga gaagtatata aaaaatgtat ataattattg caagcctttg ccaaaaacaa ttgatgataa ggtagctttg tgtaagatta tacaggaagg gttcccccgg attttccttg gatccgattc tgcaccccat tatagagaac acaaaaatga tcctcactat aaaccaggaa tatacacaca gccatttctc atgtcgtatg tttctcacat ttttaacaaa attaattctt tggacaaggt agaaaatttt gcctgcaaaa atgctgcaat ttttttaaat ttaaaggaga aaaaaacaga tacaaatgaa cagtcccttt ctcttcgtat acaaaagaaa ggattaaaaa ttcctgatga atattttggg gtcgtcccct ttttggctgg acaaacaatt gatttcacag cgtcgtttgt gggtgcagat gtgtga

Details for PlknA.00043.a.B1.GE37336

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Validated AA Sequence

MHCHLRQGDM LSFTVGAIKK GGCDRVLVMP NTTPIISTCE EAKKYRSELI KQDPSVDYLM TLYLNRKTDS NDILRNHEEC NLQGVKIYPS NVTTNSNHGV TTLEAYYEIF HTLEKLNKSL HIHCEEPNVN PLYAERSYVQ HIHDLAVHFP HLKIVLEHIS TVEMINLVRT YPNIAGSVTP HHLQLTIDDV VDTESYDYAP NVVTIEKYIK NVYNYCKPLP KTIDDKVALC KIIQEGFPRI FLGSDSAPHY REHKNDPHYK PGIYTQPFLM SYVSHIFNKI NSLDKVENFA CKNAAIFLNL KEKKTDTNEQ SLSLRIQKKG LKIPDEYFGV VPFLAGQTID

Validated NT Sequence

aaatcaattg tttgtccagc caaaaagggg acgaccccaa aatattcatc aggaattttt aatcctttct tttgtatacg aagagaaagg gactgttcat ttgtatctgt ttttttctcc tttaaattta aaaaaattgc agcatttttg caggcaaaat tttctacctt gtccaaagaa ttaattttgt taaaaatgtg agaaacatac gacatgagaa atggctgtgt gtatattcct ggtttatagt gaggatcatt tttgtgttct ctataatggg gtgcagaatc ggatccaagg aaaatccggg ggaacccttc ctgtataatc ttacacaaag ctaccttatc atcaattgtt tttggcaaag gcttgcaata attatataca ttttttatat acttctctat ggttaccacg ttaggagcat agtcgtagct ctccgtgtct acaacatcgt ctatggttag ttgcaaatgg tgcggagtaa ctgaacctgc gatgttcggg tatgtcctaa ccaagtttat catttctacc gtagaaatgt gttctagtac gattttcaaa tgggggaagt ggacagccaa gtcatgtata tgctgtacgt aactcctttc tgcataaagt ggattcacgt tgggttcttc acaatgtatg tgcaaacttt tgtttaactt ttcaagtgta tggaaaattt cataatatgc ttctaacgtt gtaactccat gattagagtt ggttgttaca ttgctggggt atattttaac tccttgcaaa ttgcattctt cgtggtttct gaggatgtca tttgaatctg tttttctgtt caagtataac gtcattaggt agtccacgct ggggtcctgc ttgattagct cgcttctata ttttttggct tcttcacagg tgcttatgat tggtgtggtg ttgggcatta cgagcacgcg gtcgcacccc cccttcttga ttgccccaac ggtgaagctc agcatgtcac cctgcctcag gtggcagtgc atgtcgtctc ccaggggaat ctgcag

Expected Protein Sequence

MAHHHHHHME PLQIPLGDDM HCHLRQGDML SFTVGAIKKG GCDRVLVMPN TTPIISTCEE AKKYRSELIK QDPSVDYLMT LYLNRKTDSN DILRNHEECN LQGVKIYPSN VTTNSNHGVT TLEAYYEIFH TLEKLNKSLH IHCEEPNVNP LYAERSYVQH IHDLAVHFPH LKIVLEHIST VEMINLVRTY PNIAGSVTPH HLQLTIDDVV DTESYDYAPN VVTIEKYIKN VYNYCKPLPK TIDDKVALCK IIQEGFPRIF LGSDSAPHYR EHKNDPHYKP GIYTQPFLMS YVSHIFNKIN SLDKVENFAC KNAAIFLNLK EKKTDTNEQS LSLRIQKKGL KIPDEYFGVV PFLAGQTIDF TASFVGADV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac cgctgcagat tcccctggga gacgacatgc actgccacct gaggcagggt gacatgctga gcttcaccgt tggggcaatc aagaaggggg ggtgcgaccg cgtgctcgta atgcccaaca ccacaccaat cataagcacc tgtgaagaag ccaaaaaata tagaagcgag ctaatcaagc aggaccccag cgtggactac ctaatgacgt tatacttgaa cagaaaaaca gattcaaatg acatcctcag aaaccacgaa gaatgcaatt tgcaaggagt taaaatatac cccagcaatg taacaaccaa ctctaatcat ggagttacaa cgttagaagc atattatgaa attttccata cacttgaaaa gttaaacaaa agtttgcaca tacattgtga agaacccaac gtgaatccac tttatgcaga aaggagttac gtacagcata tacatgactt ggctgtccac ttcccccatt tgaaaatcgt actagaacac atttctacgg tagaaatgat aaacttggtt aggacatacc cgaacatcgc aggttcagtt actccgcacc atttgcaact aaccatagac gatgttgtag acacggagag ctacgactat gctcctaacg tggtaaccat agagaagtat ataaaaaatg tatataatta ttgcaagcct ttgccaaaaa caattgatga taaggtagct ttgtgtaaga ttatacagga agggttcccc cggattttcc ttggatccga ttctgcaccc cattatagag aacacaaaaa tgatcctcac tataaaccag gaatatacac acagccattt ctcatgtcgt atgtttctca catttttaac aaaattaatt ctttggacaa ggtagaaaat tttgcctgca aaaatgctgc aattttttta aatttaaagg agaaaaaaac agatacaaat gaacagtccc tttctcttcg tatacaaaag aaaggattaa aaattcctga tgaatatttt ggggtcgtcc cctttttggc tggacaaaca attgatttca cagcgtcgtt tgtgggtgca gatgtgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for PlknA.00043.a.B1.PW37388

PURIFICATION DATe:	11/4/2013
CONCENTRATION:	15.9mg/ml
OBSERVED MW:	41kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAC CGCTGCAGAT TCCCCTGGGA GACGACATGC ACTGCCACCT GAGGCAGGGT GACATGCTGA GCTTCACCGT TGGGGCAATC AAGAAGGGGG GGTGCGACCG CGTGCTCGTA ATGCCCAACA CCACACCAAT CATAAGCACC TGTGAAGAAG CCAAAAAATA TAGAAGCGAG CTAATCAAGC AGGACCCCAG CGTGGACTAC CTAATGACGT TATACTTGAA CAGAAAAACA GATTCAAATG ACATCCTCAG AAACCACGAA GAATGCAATT TGCAAGGAGT TAAAATATAC CCCAGCAATG TAACAACCAA CTCTAATCAT GGAGTTACAA CGTTAGAAGC ATATTATGAA ATTTTCCATA CACTTGAAAA GTTAAACAAA AGTTTGCACA TACATTGTGA AGAACCCAAC GTGAATCCAC TTTATGCAGA AAGGAGTTAC GTACAGCATA TACATGACTT GGCTGTCCAC TTCCCCCATT TGAAAATCGT ACTAGAACAC ATTTCTACGG TAGAAATGAT AAACTTGGTT AGGACATACC CGAACATCGC AGGTTCAGTT ACTCCGCACC ATTTGCAACT AACCATAGAC GATGTTGTAG ACACGGAGAG CTACGACTAT GCTCCTAACG TGGTAACCAT AGAGAAGTAT ATAAAAAATG TATATAATTA TTGCAAGCCT TTGCCAAAAA CAATTGATGA TAAGGTAGCT TTGTGTAAGA TTATACAGGA AGGGTTCCCC CGGATTTTCC TTGGATCCGA TTCTGCACCC CATTATAGAG AACACAAAAA TGATCCTCAC TATAAACCAG GAATATACAC ACAGCCATTT CTCATGTCGT ATGTTTCTCA CATTTTTAAC AAAATTAATT CTTTGGACAA GGTAGAAAAT TTTGCCTGCA AAAATGCTGC AATTTTTTTA AATTTAAAGG AGAAAAAAAC AGATACAAAT GAACAGTCCC TTTCTCTTCG TATACAAAAG AAAGGATTAA AAATTCCTGA TGAATATTTT GGGGTCGTCC CCTTTTTGGC TGGACAAACA ATTGATTTCA CAGCGTCGTT TGTGGGTGCA GATGTGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T