PlfaA.20400.a

SNF1-related serine/threonine protein kinase KIN

CENTER ID: PlfaA.20400.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.20400.a.HX1.GE43843 full length 1 765
PlfaA.20400.a.HX2.GE43845 kinase domain 452 656
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1454300
UniProt: Q8IKT6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSSSVYCKN KQICIIKASL KNCSLKKSGV NISIPLPNNS YKKNKIVNNI YNGKDKKEEN KIHQDKYVEY EKVGYENIKE YIKSNSYKAI HLCKNNKVNM KSCFSYSNNT GDVNTIGKKD SYKKNINLTM SSNNQIKYDH IKNYNIERYK NNIIKRKSEP IGKTKLFIYN KLNEKNMYKY NHKDDHNIYH KMKERNKYNI NEKGMSKIFN THKIGKKMCK NVCSKKNLCN IQKNVFIKND NAIKYNNIYN NIYNNTKDKK EKNNGELIKS KTTMLNTPIR KCKIRHIIVS ASFINKKNIK ELNKIMLNNK HKSKLNNKEN NIINVKENIH LNDLSSNIYE EHKHNYDIHK DNKNSSMDYT NEMWIKENEY LEENKKNKYK IIVVKNKKKE IGNYIVTKKK IGKGTFGKVC LGIHIYTHEI VAIKILNKKR LIEIINYDKI IKEIEIHKNI NHNHICKFYD VYQNKNNIYM ILEYVENGNL LTYIYNNYNI NENNARRILY QLINAIEYLH EIKIVHRDLK PENILLDNNN NVKLIDFGLS TIYRKNCLLT TSCGSPFYTS PEILLGQKYE AELTDVWSLG IILFLLLNHR LPFNNSDMNK LFTQIIKGIL YFQPYVSKNA KHLLQNMLNV NFKKRFTMNK IKNHIWFTTH PMKIMLSNNI STTCNLIDCN TCWYKYIIYK KNNSYYNDMI INKISKMYNI NKRYINKQLI NKHKNYVKTA YDLLINKIIR NLANHGDIYS RHLIITRNGH NNIQERIFHY KTDGL
NT Sequence
atgccatcat cctctgttta ttgtaaaaac aaacaaatat gtattataaa agcaagcctc aaaaattgtt cattaaaaaa aagtggagtt aatataagta tacccctccc aaacaatagt tataaaaaaa ataaaatagt aaataatata tataatggaa aagacaagaa agaggaaaat aaaatacatc aagataaata tgtagaatat gaaaaggtgg ggtatgaaaa tataaaagaa tatataaaaa gtaactccta caaagcaatt catttatgta aaaataataa ggtgaatatg aaatcttgtt tttcatattc aaataataca ggggatgtta atacaatagg aaaaaaggat agttataaaa aaaatattaa ccttacaatg tcatcaaata atcaaataaa atatgatcat attaaaaact ataatattga aaggtataaa aataatatta taaaaaggaa aagtgaacct ataggaaaaa caaaattatt tatatataat aaattaaatg aaaagaacat gtacaaatat aatcataaag atgatcacaa tatttatcat aaaatgaaag aaagaaataa atataatata aacgaaaaag gtatgtctaa aatttttaat actcataaaa ttggtaagaa aatgtgtaaa aatgtatgtt caaagaaaaa tttatgtaat attcaaaaga atgtttttat aaaaaatgac aacgccatta aatataataa tatttataat aatatctata ataacacaaa ggataaaaaa gaaaaaaata atggggaatt aataaaatct aaaacaacca tgttaaacac acccataagg aagtgcaaaa taagacatat tattgtgagt gcaagtttta taaataaaaa gaatatcaaa gaattaaata aaataatgtt gaataataaa cacaaaagta aactaaataa taaagaaaat aatataatca atgtaaaaga aaatattcat ttaaatgatc tatctagtaa tatatatgaa gaacacaaac ataactatga tatccataag gataataaaa attcatctat ggattatacg aatgaaatgt ggataaaaga aaatgaatat ttagaagaaa acaaaaaaaa taaatataaa attattgttg taaaaaataa aaagaaagaa attggaaatt atattgtcac gaaaaaaaaa attgggaaag ggacgtttgg taaagtttgt ttaggtatac atatatatac acacgaaatt gttgctatca aaatattgaa taaaaaaagg ttaattgaaa ttataaatta tgataagata ataaaagaaa tagaaataca taaaaatatt aatcataatc atatatgtaa attttatgat gtatatcaga ataaaaataa tatatatatg atattagaat atgtagaaaa cggtaaccta ctaacctata tttataataa ttataatatt aatgaaaata atgctaggag aattttatat caattaataa atgcaatcga atatttacat gagataaaga tagtacaccg agatttaaaa cctgaaaata ttttattaga taataataat aatgtgaaat taatagattt tggtctatca acaatttata gaaaaaattg tttattaact acatcatgtg gttctccttt ttatacctct cctgaaatat tacttggaca aaaatatgaa gctgagttaa cagatgtgtg gtccctaggt attattcttt ttttattact aaaccatcgt cttcctttta ataatagtga tatgaataaa ttatttacac aaatcataaa aggtatatta tattttcaac cttatgtatc taaaaatgca aaacacttat tacaaaatat gttaaatgta aattttaaaa aacgttttac aatgaataaa attaaaaatc atatatggtt tactacacat cctatgaaaa tcatgctatc aaataatatt tcaacgactt gtaatttaat tgattgtaat acgtgttggt acaaatatat aatatataaa aaaaataatt cttattacaa cgatatgatt ataaataaaa taagtaaaat gtataatata aataaacgat atataaataa acaattaata aataaacata agaattatgt aaaaacagct tatgatcttt tgataaataa aattatcaga aatttagcaa accatgggga catatattca cgacatctaa ttattacaag aaacggtcat aataatattc aagaaagaat ttttcattat aagacagatg gattatga
Details for PlfaA.20400.a.HX1.GE43843
HARVESTED ON: data unavailable
SEQUENCED ON: 8/28/2020
EXPECTED MW: 148kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK NKEILAVVEA VSNEKALPRE KIFEALESAL ATATKKKYEQ EIDVRVQIDR KSGDFDTFRR WLVVDEVTQP TKEITLEAAR YEDESLNLGD YVEDQIESVT FDRITTQTAK QVIVQKVREA ERAMVVDQFR EHEGEIITGV VKKVNRDNIS LDLGNNAEAV ILREDMLPRE NFRPGDRVRG VLYSVRPEAR GAQLFVTRSK PEMLIELFRI EVPEIGEEVI EIKAAARDPG SRAKIAVKTN DKRIDPVGAC VGMRGARVQA VSTELGGERI DIVLWDDNPA QFVINAMAPA DVASIVVDED KHTMDIAVEA GNLAQAIGRN GQNVRLASQL SGWELNVMTV DDLQAKHQAE AHAAIDTFTK YLDIDEDFAT VLVEEGFSTL EELAYVPMKE LLEIEGLDEP TVEALRERAK NALATIAQAQ EESLGDNKPA DDLLNLEGVD RDLAFKLAAR GVCTLEDLAE QGIDDLADIE GLTDEKAGAL IMAARNICWF GDEAGTLEAQ TQGPGSMPSS SVYCKNKQIC IIKASLKNCS LKKSGVNISI PLPNNSYKKN KIVNNIYNGK DKKEENKIHQ DKYVEYEKVG YENIKEYIKS NSYKAIHLCK NNKVNMKSCF SYSNNTGDVN TIGKKDSYKK NINLTMSSNN QIKYDHIKNY NIERYKNNII KRKSEPIGKT KLFIYNKLNE KNMYKYNHKD DHNIYHKMKE RNKYNINEKG MSKIFNTHKI GKKMCKNVCS KKNLCNIQKN VFIKNDNAIK YNNIYNNIYN NTKDKKEKNN GELIKSKTTM LNTPIRKCKI RHIIVSASFI NKKNIKELNK IMLNNKHKSK LNNKENNIIN VKENIHLNDL SSNIYEEHKH NYDIHKDNKN SSMDYTNEMW IKENEYLEEN KKNKYKIIVV KNKKKEIGNY IVTKKKIGKG TFGKVCLGIH IYTHEIVAIK ILNKKRLIEI INYDKIIKEI EIHKNINHNH ICKFYDVYQN KNNIYMILEY VENGNLLTYI YNNYNINENN ARRILYQLIN AIEYLHEIKI VHRDLKPENI LLDNNNNVKL IDFGLSTIYR KNCLLTTSCG SPFYTSPEIL LGQKYEAELT DVWSLGIILF LLLNHRLPFN NSDMNKLFTQ IIKGILYFQP YVSKNAKHLL QNMLNVNFKK RFTMNKIKNH IWFTTHPMKI MLSNNISTTC NLIDCNTCWY KYIIYKKNNS YYNDMIINKI SKMYNINKRY INKQLINKHK NYVKTAYDLL INKIIRNLAN HGDIYSRHLI ITRNGHNNIQ ERIFHYKTDG L
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagaacaa agaaattttg gctgtagttg aagccgtatc caatgaaaag gcgctacctc gcgagaagat tttcgaagca ttggaaagcg cgctggcgac agcaacaaag aaaaaatatg aacaagagat cgacgtccgc gtacagatcg atcgcaaaag cggtgatttt gacactttcc gtcgctggtt agttgttgat gaagtcaccc agccgaccaa ggaaatcacc cttgaagccg cacgttatga agatgaaagc ctgaacctgg gcgattacgt tgaagatcag attgagtctg ttacctttga ccgtatcact acccagacgg caaaacaggt tatcgtgcag aaagtgcgtg aagccgaacg tgcgatggtg gttgatcagt tccgtgaaca cgaaggtgaa atcatcaccg gcgtggtgaa aaaagtaaac cgcgacaaca tctctctgga tctgggcaac aacgctgaag ccgtgatcct gcgcgaagat atgctgccgc gtgaaaactt ccgccctggc gaccgcgttc gtggcgtgct ctattccgtt cgcccggaag cgcgtggcgc gcaactgttc gtcactcgtt ccaagccgga aatgctgatc gaactgttcc gtattgaagt gccagaaatc ggcgaagaag tgattgaaat taaagcagcg gctcgcgatc cgggttctcg tgcgaaaatc gcggtgaaaa ccaacgataa acgtatcgat ccggtaggtg cttgcgtagg tatgcgtggc gcgcgtgttc aggcggtgtc tactgaactg ggtggcgagc gtatcgatat cgtcctgtgg gatgataacc cggcgcagtt cgtgattaac gcaatggcac cggcagacgt tgcttctatc gtggtggatg aagataaaca caccatggat atcgccgttg aagccggtaa cctggcgcag gcgattggcc gtaacggtca gaacgtgcgt ctggcttcgc agctgagcgg ttgggaactc aacgtgatga ccgttgacga cctgcaggct aagcatcagg cggaagcgca cgcagcgatc gacaccttca ccaaatatct cgacatcgac gaagacttcg cgactgttct ggtagaagaa ggcttctcga cgctggaaga attggcctat gtgccgatga aagagctgtt ggaaatcgaa ggccttgatg agccgaccgt tgaagcactg cgcgagcgtg ctaaaaatgc actggccacc attgcacagg cccaggaaga aagcctcggt gataacaaac cggctgacga tctgctgaac cttgaagggg tagatcgtga tttggcattc aaactggccg cccgtggcgt ttgtacgctg gaagatctcg ccgaacaggg cattgatgat ctggctgata tcgaagggtt gaccgacgaa aaagccggag cactgattat ggctgcccgt aacatttgct ggttcggtga cgaagcgggt accctggaag ctcagaccca gggtcctggt tcgatgccat catcctctgt ttattgtaaa aacaaacaaa tatgtattat aaaagcaagc ctcaaaaatt gttcattaaa aaaaagtgga gttaatataa gtatacccct cccaaacaat agttataaaa aaaataaaat agtaaataat atatataatg gaaaagacaa gaaagaggaa aataaaatac atcaagataa atatgtagaa tatgaaaagg tggggtatga aaatataaaa gaatatataa aaagtaactc ctacaaagca attcatttat gtaaaaataa taaggtgaat atgaaatctt gtttttcata ttcaaataat acaggggatg ttaatacaat aggaaaaaag gatagttata aaaaaaatat taaccttaca atgtcatcaa ataatcaaat aaaatatgat catattaaaa actataatat tgaaaggtat aaaaataata ttataaaaag gaaaagtgaa cctataggaa aaacaaaatt atttatatat aataaattaa atgaaaagaa catgtacaaa tataatcata aagatgatca caatatttat cataaaatga aagaaagaaa taaatataat ataaacgaaa aaggtatgtc taaaattttt aatactcata aaattggtaa gaaaatgtgt aaaaatgtat gttcaaagaa aaatttatgt aatattcaaa agaatgtttt tataaaaaat gacaacgcca ttaaatataa taatatttat aataatatct ataataacac aaaggataaa aaagaaaaaa ataatgggga attaataaaa tctaaaacaa ccatgttaaa cacacccata aggaagtgca aaataagaca tattattgtg agtgcaagtt ttataaataa aaagaatatc aaagaattaa ataaaataat gttgaataat aaacacaaaa gtaaactaaa taataaagaa aataatataa tcaatgtaaa agaaaatatt catttaaatg atctatctag taatatatat gaagaacaca aacataacta tgatatccat aaggataata aaaattcatc tatggattat acgaatgaaa tgtggataaa agaaaatgaa tatttagaag aaaacaaaaa aaataaatat aaaattattg ttgtaaaaaa taaaaagaaa gaaattggaa attatattgt cacgaaaaaa aaaattggga aagggacgtt tggtaaagtt tgtttaggta tacatatata tacacacgaa attgttgcta tcaaaatatt gaataaaaaa aggttaattg aaattataaa ttatgataag ataataaaag aaatagaaat acataaaaat attaatcata atcatatatg taaattttat gatgtatatc agaataaaaa taatatatat atgatattag aatatgtaga aaacggtaac ctactaacct atatttataa taattataat attaatgaaa ataatgctag gagaatttta tatcaattaa taaatgcaat cgaatattta catgagataa agatagtaca ccgagattta aaacctgaaa atattttatt agataataat aataatgtga aattaataga ttttggtcta tcaacaattt atagaaaaaa ttgtttatta actacatcat gtggttctcc tttttatacc tctcctgaaa tattacttgg acaaaaatat gaagctgagt taacagatgt gtggtcccta ggtattattc tttttttatt actaaaccat cgtcttcctt ttaataatag tgatatgaat aaattattta cacaaatcat aaaaggtata ttatattttc aaccttatgt atctaaaaat gcaaaacact tattacaaaa tatgttaaat gtaaatttta aaaaacgttt tacaatgaat aaaattaaaa atcatatatg gtttactaca catcctatga aaatcatgct atcaaataat atttcaacga cttgtaattt aattgattgt aatacgtgtt ggtacaaata tataatatat aaaaaaaata attcttatta caacgatatg attataaata aaataagtaa aatgtataat ataaataaac gatatataaa taaacaatta ataaataaac ataagaatta tgtaaaaaca gcttatgatc ttttgataaa taaaattatc agaaatttag caaaccatgg ggacatatat tcacgacatc taattattac aagaaacggt cataataata ttcaagaaag aatttttcat tataagacag atggattaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for PlfaA.20400.a.HX2.GE43845
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 82kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK NKEILAVVEA VSNEKALPRE KIFEALESAL ATATKKKYEQ EIDVRVQIDR KSGDFDTFRR WLVVDEVTQP TKEITLEAAR YEDESLNLGD YVEDQIESVT FDRITTQTAK QVIVQKVREA ERAMVVDQFR EHEGEIITGV VKKVNRDNIS LDLGNNAEAV ILREDMLPRE NFRPGDRVRG VLYSVRPEAR GAQLFVTRSK PEMLIELFRI EVPEIGEEVI EIKAAARDPG SRAKIAVKTN DKRIDPVGAC VGMRGARVQA VSTELGGERI DIVLWDDNPA QFVINAMAPA DVASIVVDED KHTMDIAVEA GNLAQAIGRN GQNVRLASQL SGWELNVMTV DDLQAKHQAE AHAAIDTFTK YLDIDEDFAT VLVEEGFSTL EELAYVPMKE LLEIEGLDEP TVEALRERAK NALATIAQAQ EESLGDNKPA DDLLNLEGVD RDLAFKLAAR GVCTLEDLAE QGIDDLADIE GLTDEKAGAL IMAARNICWF GDEAGTLEAQ TQGPGSHNHI CKFYDVYQNK NNIYMILEYV ENGNLLTYIY NNYNINENNA RRILYQLINA IEYLHEIKIV HRDLKPENIL LDNNNNVKLI DFGLSTIYRK NCLLTTSCGS PFYTSPEILL GQKYEAELTD VWSLGIILFL LLNHRLPFNN SDMNKLFTQI IKGILYFQPY VSKNAKHLLQ NMLNVNFKKR FTMNKIKNHI WFTTHPMKIM L
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagaacaa agaaattttg gctgtagttg aagccgtatc caatgaaaag gcgctacctc gcgagaagat tttcgaagca ttggaaagcg cgctggcgac agcaacaaag aaaaaatatg aacaagagat cgacgtccgc gtacagatcg atcgcaaaag cggtgatttt gacactttcc gtcgctggtt agttgttgat gaagtcaccc agccgaccaa ggaaatcacc cttgaagccg cacgttatga agatgaaagc ctgaacctgg gcgattacgt tgaagatcag attgagtctg ttacctttga ccgtatcact acccagacgg caaaacaggt tatcgtgcag aaagtgcgtg aagccgaacg tgcgatggtg gttgatcagt tccgtgaaca cgaaggtgaa atcatcaccg gcgtggtgaa aaaagtaaac cgcgacaaca tctctctgga tctgggcaac aacgctgaag ccgtgatcct gcgcgaagat atgctgccgc gtgaaaactt ccgccctggc gaccgcgttc gtggcgtgct ctattccgtt cgcccggaag cgcgtggcgc gcaactgttc gtcactcgtt ccaagccgga aatgctgatc gaactgttcc gtattgaagt gccagaaatc ggcgaagaag tgattgaaat taaagcagcg gctcgcgatc cgggttctcg tgcgaaaatc gcggtgaaaa ccaacgataa acgtatcgat ccggtaggtg cttgcgtagg tatgcgtggc gcgcgtgttc aggcggtgtc tactgaactg ggtggcgagc gtatcgatat cgtcctgtgg gatgataacc cggcgcagtt cgtgattaac gcaatggcac cggcagacgt tgcttctatc gtggtggatg aagataaaca caccatggat atcgccgttg aagccggtaa cctggcgcag gcgattggcc gtaacggtca gaacgtgcgt ctggcttcgc agctgagcgg ttgggaactc aacgtgatga ccgttgacga cctgcaggct aagcatcagg cggaagcgca cgcagcgatc gacaccttca ccaaatatct cgacatcgac gaagacttcg cgactgttct ggtagaagaa ggcttctcga cgctggaaga attggcctat gtgccgatga aagagctgtt ggaaatcgaa ggccttgatg agccgaccgt tgaagcactg cgcgagcgtg ctaaaaatgc actggccacc attgcacagg cccaggaaga aagcctcggt gataacaaac cggctgacga tctgctgaac cttgaagggg tagatcgtga tttggcattc aaactggccg cccgtggcgt ttgtacgctg gaagatctcg ccgaacaggg cattgatgat ctggctgata tcgaagggtt gaccgacgaa aaagccggag cactgattat ggctgcccgt aacatttgct ggttcggtga cgaagcgggt accctggaag ctcagaccca gggtcctggt tcgcataatc atatatgtaa attttatgat gtatatcaga ataaaaataa tatatatatg atattagaat atgtagaaaa cggtaaccta ctaacctata tttataataa ttataatatt aatgaaaata atgctaggag aattttatat caattaataa atgcaatcga atatttacat gagataaaga tagtacaccg agatttaaaa cctgaaaata ttttattaga taataataat aatgtgaaat taatagattt tggtctatca acaatttata gaaaaaattg tttattaact acatcatgtg gttctccttt ttatacctct cctgaaatat tacttggaca aaaatatgaa gctgagttaa cagatgtgtg gtccctaggt attattcttt ttttattact aaaccatcgt cttcctttta ataatagtga tatgaataaa ttatttacac aaatcataaa aggtatatta tattttcaac cttatgtatc taaaaatgca aaacacttat tacaaaatat gttaaatgta aattttaaaa aacgttttac aatgaataaa attaaaaatc atatatggtt tactacacat cctatgaaaa tcatgctaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa