PlfaA.20309.a

Phosphatidylinositol 4-kinase (EC 2.7.1.67) (PI4K)

CENTER ID: PlfaA.20309.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.20309.a.DB11.GE43774 full length 1 1559
PlfaA.20309.a.DB13.GE44580 full length 104 1559
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.20309.a.DB12.PD38452 full length 1122 1559
PlfaA.20309.a.DB21.PD38483 full length 1 1559

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_0509800
UniProt: Q8I406

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEDDKDNNIT KENLNEVDIE NSQHPIIEEK NLKEEGVKDE GGKVFLKGEP IHKHLYKHSY NTQVYKDEVT HMRLRNRENK NLNENNSDDN KKVNKKNGCV DINMNEHTNK KEDGDDDDGD DDDGDDDDDD DDDDGDDDGD DDGDDDGDDG DDDSNSNDDI KNNHKYNNDD YHKNMIKLHC TSPKKKEHSI NNNNIVSHNE IEENLKDHTL ITNDEEKNIK IIEMYEKKLE NEEIKKQNKE DANENNKSQI RESISKIFKE CPTSKPKIIN EINNETKYDN NNNNNNNNNN NNNNSNNNNK NNNKNNNKNS SYNNYYYNNI YNCNNMKENN HIDGDKNNIY ETNNYSDSEK NKSSDTEKNK LKLNNKNTLK EGSLLRLFRC EYFDTHLHIR YLYDRKEVGV HEYLVNSLYT QRKYEDILFY LPQLSQISLV RYESSSLYRF LLEKASKSMH FALKLSWLYH SIVEDNSSKY KDLAQRMIQE IEMAVVNCKP LNNECKSFEE NKQSYLLDLA YPLLFKRKFI LKKIKTNEKL NKIKMYNKCL NNSYNSYTLK NLHVSNPIEM DTTSFLRNHK NSNDSTLSQG LPNKSSNDDN CNVLKREDHQ DVINISSEEN EERDINKNLK EEVTTLDDNK ISRENNLQNL QSEKKTPILK RKNLKQAHTT PCPKLPPCYI INSGSLSIAR AKVKLPSTYS KLGNPLSFSK FSLPDFNYSY DMIEELQQFF MKQRRCDYFS LLNNFINLLI SVSNLLAAEP DIDLRNELLR RFIYSLNSWM NMRRCIVACC ENIFAMTGLC IPLESMSSST FNHDTNNRLS YNNLQILHFN NEECKIFFSK KRAPYLLMFE VADLDEDISH ISDNIFYTKK SLFFQQDNNN NNNNDNNNNN NNNNNNNNNY NCNNNNYNSD VQSKEPKNVR EEYSRKSLIL SRNFCCSKNY GYYEENLNTF DYENRLSLED KKKKNSKKKK YAKEQEQTNN EKLKDKEKER KKKNIFDLNS LKMDDIYVYN CIVNDLRREN LISFTSKEDE NLALIKKCLG LKMVEEEKNE NDDDDNDDDN DDDNDDDNDD YNEEEEDDNY EKKNFEEEQK GNYNNDNINH NEKKKKVPTA NDNLSSSSFC VKKNYLLNSR SVSMPSYLNN NLEKCDTSSY DNTSNNIKTS IETISNGIDD IQVEKNIEKN ETFEEMKRKN SNNEFIDYNE HMVKLSPYYT TTNHRGKNKN DDTDDNSDDD NINNYNNNNN SGNNNQDNTI SNFPNNILDL SEYFKPENYL NEEFKKKNCK IIKRLLWGEL FEEKKKRIRK VSPYGKLKTW DLKCVIIKGG DDLRQELLAS QLIKQFKIIF ENAGLPLWLR PYEILVTGSN SGIIEYVNDT CSVDSLKRKF GADSISTIFN IVFSDYIFEA KKNFIESHAA YSLISYLLQV KDRHNGNLLL DSDGHLIHID YGFMLTNSPG NVNFETSPFK LTQEYLDIMD GEKSDNYEYF RRLIVSGFLE ARKHSEEIIL FVELMMPALK IPCFANGTQF CIESLKERFM TNLTVDVCIQ RINALIEASI NNFRSVQYDY FQRITNGIM
NT Sequence
atggaggatg ataaagataa caacataaca aaagaaaatc tgaatgaagt tgacattgaa aatagtcaac atcctataat agaagaaaaa aatttaaaag aggaaggagt aaaagatgag gggggaaagg tttttttaaa aggtgaaccg atacataaac atttatacaa acatagttat aatacgcaag tatataaaga tgaagttaca catatgcgtt taagaaatag agaaaataaa aatttgaatg aaaataattc ggatgataat aaaaaggtaa ataagaagaa tgggtgtgtt gatattaata tgaatgaaca tactaataaa aaggaagatg gtgatgatga tgatggtgat gatgatgatg gtgatgatga tgatgatgat gatgatgatg atggtgatga tgatggtgat gatgatggtg atgatgatgg tgatgatggt gatgatgata gtaatagtaa cgatgatatt aagaataatc ataagtacaa taatgatgat tatcataaaa atatgataaa gttacattgc acatcgccca aaaaaaagga acattccatt aataataata atattgtatc acataacgag attgaagaaa atttaaaaga ccatacttta atcacgaacg atgaagaaaa gaatattaaa ataattgaga tgtatgaaaa aaaattagaa aatgaagaaa taaaaaaaca aaataaagaa gacgctaacg aaaataataa aagtcaaata agagaaagca tttctaaaat ttttaaggaa tgtcctacat ctaaaccgaa aataattaat gaaataaata atgagacaaa atatgataat aataataata ataataataa taataataat aataataata atagtaataa taataataag aacaataata agaacaataa taagaacagt agttataata attattatta taataatatt tataattgta ataatatgaa agaaaataat catattgatg gagataaaaa taatatatat gaaactaata attatagtga ttctgaaaaa aacaaaagta gtgatacaga aaaaaataaa ttaaaattga ataataagaa tacattaaaa gaaggtagtt tgttaagatt atttagatgt gaatattttg atactcattt acatattaga tatttatatg atagaaaaga agttggtgta catgaatatt tagttaattc tttatatact caaagaaaat atgaagatat attattttat ttacctcaat taagtcaaat atctttagta agatatgaat cttcatctct ttatcgtttc ttattagaaa aagctagtaa gtcaatgcat tttgccttaa aattatcatg gttatatcat tccattgttg aagataattc tagtaaatat aaagatttag ctcaacgaat gatacaagaa attgaaatgg ctgttgttaa ttgtaaacct ttaaataatg aatgtaagtc ttttgaagaa aataaacaat cttatcttct tgatttggct tatccacttt tattcaaaag aaaatttatt ttaaaaaaaa ttaaaactaa tgaaaaatta aacaaaataa aaatgtataa taaatgcctt aacaattcat ataattcata tacattaaaa aatttgcatg tcagtaatcc aatagaaatg gacactacat catttttacg taatcataaa aattcaaatg atagtacact ttctcaagga ctacctaata aatctagcaa tgatgataat tgtaatgtgc ttaagagaga agatcaccaa gatgtcataa atatatcttc agaagaaaat gaagaaagag atataaataa aaatctcaag gaagaagtta ctacgttaga tgacaataaa atatctcgtg aaaataatct acaaaatctt caatcagaaa aaaaaacacc tatattaaaa agaaagaatt taaaacaagc acatacgaca ccatgtccta aacttcctcc ttgttatatt ataaattctg gctcgttatc tattgctagg gctaaagtaa aattacctag tacttattcc aaattaggta atcctttaag cttttctaaa ttttcacttc ctgattttaa ttatagttat gatatgatag aagaattaca acagtttttt atgaaacaaa gacgatgtga ttattttagt ttattaaata attttattaa tttgcttata tcggtttcta atttattagc tgctgagcca gatattgatc tacgcaatga actgttaaga cgatttatat attctttaaa tagctggatg aatatgagaa gatgtatagt ggcttgttgt gaaaatattt ttgccatgac aggtttgtgt ataccattag aaagtatgtc ttcctcaaca tttaatcatg atacaaataa tcgtttaagt tataataatt tacaaatttt acattttaat aatgaagaat gtaaaatatt tttctccaaa aagagagcac catatttatt aatgtttgaa gttgctgatt tggatgaaga tattagtcac atttctgata atatttttta tactaaaaaa agtttatttt ttcagcaaga taataataat aataataata atgacaacaa caacaacaac aacaacaaca ataataataa taataattac aattgtaata ataataatta taatagtgat gtacaaagca aagaaccaaa aaatgtaaga gaagaatata gtagaaagag cctcattttg tcaagaaatt tttgttgttc aaaaaattat ggatattatg aagagaattt gaatacattc gattatgaaa acagattaag cttagaagat aaaaaaaaaa agaacagtaa gaaaaaaaaa tatgcaaaag aacaagaaca aacgaataat gaaaaattga aggataaaga aaaagagaga aagaaaaaaa atatttttga tttaaattct ttaaaaatgg atgatatata tgtatataat tgtattgtaa acgatttgag aagagaaaat ttgatatcgt ttacgtctaa agaggatgaa aatttagcac ttataaaaaa atgtctagga ctaaaaatgg tagaagaaga aaaaaacgaa aatgatgacg atgataatga cgatgataat gacgatgaca atgacgatga caatgacgat tacaatgagg aggaggagga tgataattat gaaaagaaaa actttgaaga agaacaaaag ggaaattata ataatgataa tattaatcat aatgaaaaaa aaaaaaaagt tccaacagca aatgataatt tatcttcatc atctttttgt gttaaaaaaa attatttatt aaattctaga tctgtttcaa tgccaagtta tttgaataac aatcttgaaa aatgtgatac aagttcatat gataatacaa gtaataatat aaaaacatcg atagaaacta taagcaatgg tatagatgat attcaagttg aaaaaaatat agagaagaat gaaacatttg aagaaatgaa gagaaaaaat tcaaataatg aatttattga ttataatgaa catatggtaa aattatctcc atattataca acaacaaatc acagagggaa aaataaaaat gatgacactg atgataatag cgatgatgat aatataaata attataataa taataataat agtggtaata ataaccaaga taataccatt agtaacttcc caaacaatat attagatctg agtgaatatt ttaaacctga aaattattta aatgaagaat ttaaaaaaaa aaattgtaaa attattaaaa gattattatg gggtgaattg tttgaagaaa aaaaaaaaag aataagaaaa gtttctcctt atggaaaatt aaaaacgtgg gatctaaaat gtgttataat aaaaggtgga gatgatttaa gacaagaatt attagcttca caactaatta aacaatttaa aattattttt gaaaatgctg gattaccatt atggttaaga ccatatgaaa tacttgtaac aggctccaat tctgggataa tagagtatgt taatgatact tgttcagttg attcattaaa aagaaaattt ggagcagata gtatttctac aatttttaat atagtctttt cggattatat ttttgaggca aaaaagaact ttattgagag ccatgctgca tattcattaa tttcatattt actccaagta aaggatcgac ataatggaaa tttactccta gattcagatg gtcatttaat tcacatagat tatggcttta tgttaacaaa ttcaccaggt aatgtgaatt ttgaaacatc cccattcaaa ttaacacaag aatatttaga tattatggat ggagaaaaat cagataatta tgaatatttc agaagactta ttgttagtgg ctttctagag gctagaaaac attcagaaga aattattctt ttcgttgaat taatgatgcc agctttaaaa attccatgtt ttgcaaacgg tacacaattc tgtatagaat ccttgaaaga aaggtttatg accaatctga cagtcgatgt gtgtattcaa cgaattaatg ccttaattga agcgtccatt aataattttc gaagtgtcca gtatgactac tttcaaagaa taacaaatgg aattatgtga
Details for PlfaA.20309.a.DB11.GE43774
HARVESTED ON: 7/9/2020
SEQUENCED ON: 6/6/2023
EXPECTED MW: 186kDa
OBSERVED MW: 186kDa
ANTIBIOTIC MARKER:
COUNT OF EXPRESSION COLONIES: Data Unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: Tni cells
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MEDDKDNNIT KENLNEVDIE NSQHPIIEEK NLKEEGVKDE GGKVFLKGEP IHKHLYKHSY NTQVYKDEVT HMRLRNRENK NLNENNSDDN KKVNKKNGCV DINMNEHTNK KEDGDDDDGD DDDGDDDDDD DDDDGDDDGD DDGDDDGDDG DDDSNSNDDI KNNHKYNNDD YHKNMIKLHC TSPKKKEHSI NNNNIVSHNE IEENLKDHTL ITNDEEKNIK IIEMYEKKLE NEEIKKQNKE DANENNKSQI RESISKIFKE CPTSKPKIIN EINNETKYDN NNNNNNNNNN NNNNSNNNNK NNNKNNNKNS SYNNYYYNNI YNCNNMKENN HIDGDKNNIY ETNNYSDSEK NKSSDTEKNK LKLNNKNTLK EGSLLRLFRC EYFDTHLHIR YLYDRKEVGV HEYLVNSLYT QRKYEDILFY LPQLSQISLV RYESSSLYRF LLEKASKSMH FALKLSWLYH SIVEDNSSKY KDLAQRMIQE IEMAVVNCKP LNNECKSFEE NKQSYLLDLA YPLLFKRKFI LKKIKTNEKL NKIKMYNKCL NNSYNSYTLK NLHVSNPIEM DTTSFLRNHK NSNDSTLSQG LPNKSSNDDN CNVLKREDHQ DVINISSEEN EERDINKNLK EEVTTLDDNK ISRENNLQNL QSEKKTPILK RKNLKQAHTT PCPKLPPCYI INSGSLSIAR AKVKLPSTYS KLGNPLSFSK FSLPDFNYSY DMIEELQQFF MKQRRCDYFS LLNNFINLLI SVSNLLAAEP DIDLRNELLR RFIYSLNSWM NMRRCIVACC ENIFAMTGLC IPLESMSSST FNHDTNNRLS YNNLQILHFN NEECKIFFSK KRAPYLLMFE VADLDEDISH ISDNIFYTKK SLFFQQDNNN NNNNDNNNNN NNNNNNNNNY NCNNNNYNSD VQSKEPKNVR EEYSRKSLIL SRNFCCSKNY GYYEENLNTF DYENRLSLED KKKKNSKKKK YAKEQEQTNN EKLKDKEKER KKKNIFDLNS LKMDDIYVYN CIVNDLRREN LISFTSKEDE NLALIKKCLG LKMVEEEKNE NDDDDNDDDN DDDNDDDNDD YNEEEEDDNY EKKNFEEEQK GNYNNDNINH NEKKKKVPTA NDNLSSSSFC VKKNYLLNSR SVSMPSYLNN NLEKCDTSSY DNTSNNIKTS IETISNGIDD IQVEKNIEKN ETFEEMKRKN SNNEFIDYNE HMVKLSPYYT TTNHRGKNKN DDTDDNSDDD NINNYNNNNN SGNNNQDNTI SNFPNNILDL SEYFKPENYL NEEFKKKNCK IIKRLLWGEL FEEKKKRIRK VSPYGKLKTW DLKCVIIKGG DDLRQELLAS QLIKQFKIIF ENAGLPLWLR PYEILVTGSN SGIIEYVNDT CSVDSLKRKF GADSISTIFN IVFSDYIFEA KKNFIESHAA YSLISYLLQV KDRHNGNLLL DSDGHLIHID YGFMLTNSPG NVNFETSPFK LTQEYLDIMD GEKSDNYEYF RRLIVSGFLE ARKHSEEIIL FVELMMPALK IPCFANGTQF CIESLKERFM TNLTVDVCIQ RINALIEASI NNFRSVQYDY FQRITNGIM
Full NT Sequence (Expression Vector + Insert)
ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatccatg agctactacc accaccacca ccaccacgac tacgacatcc cgactactga aaacttgtac ttccagggcg ctatggaccc ggaattcatg gaggacgaca aggataacaa catcaccaag gagaacctga acgaagtgga catcgagaac tcccagcacc ccatcatcga ggaaaagaac ctgaaggagg aaggtgtgaa ggacgagggt ggcaaggtct tcctgaaggg cgaaccaatc cacaagcacc tgtacaagca ctcctacaac actcaggtgt acaaggacga ggtcacccac atgaggctga gaaaccgcga aaacaagaac ctgaacgaga acaacagcga cgacaacaag aaggtcaaca agaagaacgg ttgcgtcgac atcaacatga acgaacacac taacaagaag gaggacggag acgacgacga cggtgacgac gacgacggcg acgatgatga cgatgatgat gatgatgatg gtgacgacga cggcgacgac gacggagacg atgatggcga cgacggagac gacgactcta actcaaacga cgacatcaag aacaaccaca agtacaacaa cgacgactac cacaagaaca tgatcaagct gcactgcacc agccctaaga agaaggaaca ctctatcaac aacaacaaca ttgtgtctca caacgagatc gaggaaaacc tgaaggacca cactctgatc accaacgacg aggaaaagaa catcaagatc atcgagatgt acgaaaagaa gctggagaac gaggaaatca agaagcagaa caaggaagac gctaacgaga acaacaagtc tcagatccgt gaatccatca gcaagatctt caaggagtgc cccacttcaa agccaaagat catcaacgaa atcaacaacg aaaccaagta cgacaataat aacaataata acaataacaa taacaacaac aacaacaact ctaacaacaa caacaagaat aataacaaga acaacaacaa gaactccagc tacaacaact actactacaa caacatctac aactgtaaca acatgaagga gaacaaccac atcgacggcg acaagaacaa catctacgaa actaacaact actctgactc agagaagaac aagtcttcag acactgaaaa gaacaagctg aagctgaaca acaagaacac tctgaaggag ggctctctgc tgagactgtt ccgctgcgaa tacttcgaca ctcacctgca catccgttac ctgtacgaca ggaaggaagt gggagtccac gagtacctgg tgaactcact gtacacccag cgtaagtacg aggacatcct gttctacctg cctcagctgt cccagatcag cctggtccgt tacgagtcca gctctctgta caggttcctg ctggaaaagg cttccaagag catgcacttc gccctgaagc tgtcatggct gtaccactcc atcgtggaag acaactcatc caagtacaag gacctggctc agcgtatgat ccaggagatc gaaatggccg tggtcaactg caagccactg aacaacgagt gcaagtcatt cgaggaaaac aagcagtcct acctgctgga cctggcttac cctctgctgt tcaagaggaa gttcatcctg aagaagatca agactaacga aaagctgaac aagatcaaga tgtacaacaa gtgcctgaac aactcttaca actcatacac cctgaagaac ctgcacgtct caaaccctat cgagatggac accacttcct tcctgcgcaa ccacaagaac tccaacgaca gcaccctgtc tcagggtctg cccaacaaga gctctaacga cgacaactgc aacgtgctga agcgtgagga ccaccaggac gtcatcaaca tctcatccga ggaaaacgag gaaagggaca tcaacaagaa cttgaaagag gaagtgacca ctctggacga caacaagatc tcaagagaga acaacctgca gaacctgcag tccgaaaaga agactcccat cctgaagcgc aagaacctga agcaggccca caccactcca tgccctaagc tgcctccctg ctacatcatc aactctggtt cactgtccat cgctcgtgcc aaggtcaagc tgccctcaac ctactccaag ctgggcaacc cactgagctt ctctaagttc agcctgcctg acttcaacta ctcttacgac atgatcgagg aactgcagca gttcttcatg aagcagcgcc gttgcgacta cttctccctg ctgaacaact tcatcaacct gctgatctca gtgtccaacc tgctggctgc cgagcccgac atcgacctga ggaacgaact gctgaggaga ttcatctaca gcctgaactc ttggatgaac atgcgccgtt gcatcgtcgc ttgctgcgaa aacatcttcg ccatgactgg cctgtgcatc ccactggagt ctatgagctc ttcaactttc aaccacgaca ccaacaacag actgtcatac aacaacctgc agatcctgca cttcaacaac gaggaatgca agatcttctt ctccaagaag cgcgctcctt acctgctgat gttcgaagtg gccgacctgg acgaggacat ctcacacatc tccgacaaca tcttctacac caagaagagc ctgttcttcc agcaggacaa caataataac aacaacaacg ataacaataa caacaataat aataataata ataacaataa caactacaac tgtaacaaca acaactacaa ctctgacgtg cagtctaagg agcctaagaa cgtcagagag gaatacagcc gcaagtctct gatcctgtca cgtaacttct gctgctccaa gaactacgga tactacgagg aaaacctgaa cactttcgac tacgaaaaca ggctgagcct ggaggataag aagaagaaga actccaagaa gaagaagtac gctaaagagc aggaacagac caacaacgag aagctgaagg ataaggagaa ggaacgcaag aagaagaaca tcttcgacct gaactccctg aagatggacg acatctacgt gtacaactgc atcgtcaacg acctgaggag agaaaacctg atctcattca cctccaagga ggacgaaaac ctggccctga tcaagaagtg cctgggactg aagatggtgg aggaagagaa gaacgagaac gacgacgacg acaacgatga tgacaacgac gacgataacg atgacgacaa cgacgactac aacgaagagg aagaggacga caactacgaa aagaagaact tcgaagagga acagaagggt aactacaaca acgataacat caaccacaac gagaagaaga agaaggtccc aactgctaac gacaacctgt ccagctcttc attctgcgtg aagaagaact acctgctgaa cagcaggtct gtctcaatgc cttcctacct gaacaacaac ctggagaagt gcgacacttc cagctacgac aacacttcta acaacattaa gactagcatc gaaaccatct ctaacggaat cgacgacatc caggtggaaa agaacatcga gaagaacgaa accttcgagg aaatgaagag aaagaactcc aacaacgagt tcatcgacta caacgagcac atggtcaagc tgagccccta ctacaccact accaaccacc gcggaaagaa caagaacgac gacactgacg acaactccga cgatgataac attaacaact acaataacaa caacaactct ggtaacaaca accaggacaa caccatctct aacttcccta acaacatcct ggacctgtca gaatacttca agcccgagaa ctacctgaac gaggagttca agaagaagaa ctgcaagatc atcaagcgtc tgctgtgggg cgaactgttc gaggagaaga agaagcgcat ccgcaaggtg tctccatacg gaaagctgaa gacttgggac ctgaagtgcg tcatcatcaa gggaggtgac gacctgcgtc aggagctgct ggcttcacag ctgatcaagc agttcaagat catcttcgag aacgccggtc tgcctctgtg gctgaggccc tacgaaatcc tggtgactgg ttccaacagc ggcatcatcg agtacgtgaa cgacacctgc agcgtcgact ctctgaagag aaagttcggc gctgactcta tctcaactat cttcaacatc gtgttctccg actacatctt cgaagccaag aagaacttca tcgagagcca cgctgcctac tcactgatct cctacctgct gcaggtcaag gaccgccaca acggaaacct gctgctggac tccgacggtc acctgatcca catcgactac ggcttcatgc tgaccaactc acccggaaac gtcaacttcg agacttcccc attcaagctg acccaggaat acctggacat catggacgga gaaaagagcg acaactacga gtacttccgc cgtctgatcg tgagcggttt cctggaagcc cgcaagcact ctgaggaaat catcctgttc gtcgagctga tgatgccagc tctgaagatc ccttgcttcg ccaacggcac ccagttctgc atcgaatctc tgaaggagag attcatgact aacctgaccg tggacgtctg catccagcgc atcaacgctc tgatcgaggc ctcaatcaac aacttccgct cggtccaata cgactacttc caaagaatca ctaacggcat tatgtgataa gcttgcggcc gcactcgagc accaccacca ccaccactaa cctaggtagc tgagcgcatg caagctgatc cgggttatta gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg atcgatcccg cacgccgata acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgagg tttaaacgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
Details for PlfaA.20309.a.DB13.GE44580
HARVESTED ON: 2/12/2021
SEQUENCED ON: 6/6/2023
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER:
COUNT OF EXPRESSION COLONIES: Data Unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: Tni cells
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MNEEFKKKNC KIIKRLLWGE LFEEKKKRIR KVSPYGKLKT WDLKCVIIKG GDDLRQELLA SQLIKQFKII FENAGLPLWL RPYEILVTGS NSGIIEYVND TCSVDSLKRK FGADSISTIF NIVFSDYIFE AKKNFIESHA AYSLISYLLQ VKDRHNGNLL LDSDGHLIHI DYGFMLTNSP GNVNFETSPF KLTQEYLDIM DGEKSDNYEY FRRLIVSGFL EARKHSEEII LFVELMMPAL KIPCFANGTQ FCIESLKERF MTNLTVDVCI QRINALIEAS INNFRSVQYD YFQRITNGIM ENLYFQGSHH HHHHHHGKPI PNPLLGLDST
Full NT Sequence (Expression Vector + Insert)
ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatccatg aacgaggaat tcaagaagaa gaactgcaag atcatcaaga ggctgctgtg gggcgaactg ttcgaggaga agaagaagcg catccgcaag gtgtcacctt acggaaagct gaagacctgg gacctgaagt gcgtcatcat caagggtggc gacgacctga ggcaggagct gctggcttcc cagctgatca agcagttcaa gatcatcttc gagaacgccg gactgcctct gtggctgcgt ccctacgaaa tcctggtgac tggatctaac tcaggtatca tcgagtacgt gaacgacacc tgctccgtcg acagcctgaa gcgcaagttc ggtgctgact ccatcagcac tatcttcaac atcgtgttct cagactacat cttcgaggcc aagaagaact tcatcgaatc ccacgctgcc tactctctga tctcatacct gctgcaggtc aaggaccgtc acaacggcaa cctgctgctg gacagcgacg gacacctgat ccacatcgac tacggtttca tgctgaccaa ctcccctggc aacgtcaact tcgagactag ccccttcaag ctgacccagg aatacctgga catcatggac ggagagaagt ctgacaacta cgaatacttc cgccgtctga tcgtgtctgg tttcctggaa gcccgcaagc actcagagga aatcatcctg ttcgtcgagc tgatgatgcc agctctgaag atcccttgct tcgccaacgg tactcagttc tgcatcgagt ccctgaagga aaggttcatg actaacctga ccgtggacgt ctgcatccag agaatcaacg ctctgatcga ggcctctatc aacaacttcc gctcagtcca gtacgactac ttccagcgta tcaccaacgg catcatggag aacctgtact tccagggttc ccatcatcac caccaccacc accacggcaa gcctatcccc aaccctctgc tgggcctgga cagcacctga taagcttgcg gccgcactcg agcaccacca ccaccaccac taacctaggt agctgagcgc atgcaagctg atccgggtta ttagtacatt tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cggatcgatc ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc gctgtcgtcg aggtttaaac gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg cca
Details for PlfaA.20309.a.DB12.PD38452
PURIFICATION DATe: 3/7/2021
CONCENTRATION: 6.6mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM tris pH 7.5, 500mM NaCl, 5% glycerol, 0.5mM TCEP, 10uM MMV390048, 150mM imidazole, EDTA-free protease inhibitor
EXPRESSION HOST: Tni cells
VIAL COUNT (approx.): 14
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
CID102838, PID8890-1, PUR2802
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MSYYHHHHHH DYDIPTTENL YFQGAMDPEF VSMPSYLNNN LEKCDTSSYD NTSNNIKTSI ETISNGIDDI QVEKNIEKNE TFEEMKRKNS NNEFIDYNEH MVKLSPYYTT TNHRGKNKND DTDDNSDDDN INNYNNNNNS GNNNQDNTIS NFPNNILDLS EYFKPENYLN EEFKKKNCKI IKRLLWGELF EEKKKRIRKV SPYGKLKTWD LKCVIIKGGD DLRQELLASQ LIKQFKIIFE NAGLPLWLRP YEILVTGSNS GIIEYVNDTC SVDSLKRKFG ADSISTIFNI VFSDYIFEAK KNFIESHAAY SLISYLLQVK DRHNGNLLLD SDGHLIHIDY GFMLTNSPGN VNFETSPFKL TQEYLDIMDG EKSDNYEYFR RLIVSGFLEA RKHSEEIILF VELMMPALKI PCFANGTQFC IESLKERFMT NLTVDVCIQR INALIEASIN NFRSVQYDYF QRITNGIM
Full NT Sequence (Expression Vector + Insert)
GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA TCAATATATA GTTGCTGATC AGATCCTCAT TGTTTGCCTC CCTGCTGCGG TTTTTCACCG AAGTTCATGC CAGTCCAGCG TTTTTGCAGC AGAAAAGCCG CCGACTTCGG TTTGCGGTCG CGAGTGAAGA TCCCTTTCTT GTTACCGCCA ACGCGCAATA TGCCTTGCGA GGTCGCAAAA TCGGCGAAAT TCCATACCTG TTCACCGACG ACGGCGCTGA CGCGATCAAA GACGCGGTGA TACATATCCA GCCATGCACA CTGATACTCT TCACTCCACA TGTCGGTGTA CATTGAGTGC AGCCCGGCTA ACGTATCCAC GCCGTATTCG GTGATGATAA TCGGCTGATG CAGTTTCTCC TGCCAGGCCA GAAGTTCTTT TTCCAGTACC TTCTCTGCCG TTTCCAAATC GCCGCTTTGG ACATACCATC CGTAATAACG GTTCAGGCAC AGCACATCAA AGAGATCGCT GATGGTATCG GTGTGAGCGT CGCAGAACAT TACATTGACG CAGGTGATCG GACGCGTCGG GTCGAGTTTA CGCGTTGCTT CCGCCAGTGG CGCGAAATAT TCCCGTGCAC CTTGCGGACG GGTATCCGGT TCGTTGGCAA TACTCCACAT CACCACGCTT GGGTGGTTTT TGTCACGCGC TATCAGCTCT TTAATCGCCT GTAAGTGCGC TTGCTGAGTT TCCCCGTTGA CTGCCTCTTC GCTGTACAGT TCTTTCGGCT TGTTGCCCGC TTCGAAACCA ATGCCTAAAG AGAGCGAAAA GCCGACAGCA GCAGTTTCAT CAATCACCAC GATGCCATGT TCATCTGCCC AGTCGAGCAT CTCTTCAGCG TAAGGGTAAT GCGAGGTACG GTAGGAGTTG GCCCCAATCC AGTCCATTAA TGCGTGGTCG TGCACCATCA GCACGTTATC GAATCCTTTG CCACGTAAGT CCGCATCTTC ATGACGACCA AAGCCAGTAA AGTAGAACGG TTTGTGGTTA ATCAGGAACT GTTGGCCCTT CACTGCCACT GACCGGATGC CGACGCGAAG CGGGTAGATA TCACACTCTG TCTGGCTTTT GGCTGTGACG CACAGTTCAT AGAGATAACC TTCACCCGGT TGCCAGAGGT GCGGATTCAC CACTTGCAAA GTCCCGCTAG TGCCTTGTCC AGTTGCAACC ACCTGTTGAT CCGCATCACG CAGTTCAACG CTGACATCAC CATTGGCCAC CACCTGCCAG TCAACAGACG CGTGGTTACA GTCTTGCGCG ACATGCGTCA CCACGGTGAT ATCGTCCACC CAGGTGTTCG GCGTGGTGTA GAGCATTACG CTGCGATGGA TTCCGGCATA GTTAAAGAAA TCATGGAAGT AAGACTGCTT TTTCTTGCCG TTTTCGTCGG TAATCACCAT TCCCGGCGGG ATAGTCTGCC AGTTCAGTTC GTTGTTCACA CAAACGGTGA TACGTACACT TTTCCCGGCA ATAACATACG GCGTGACATC GGCTTCAAAT GGCGTATAGC CGCCCTGATG CTCCATCACT TCCTGATTAT TGACCCACAC TTTGCCGTAA TGAGTGACCG CATCGAAACG CAGCACGATA CGCTGGCCTG CCCAACCTTT CGGTATAAAG ACTTCGCGCT GATACCAGAC GTTGCCCGCA TAATTACGAA TATCTGCATC GGCGAACTGA TCGTTAAAAC TGCCTGGCAC AGCAATTGCC CGGCTTTCTT GTAACGCGCT TTCCCACCAA CGCTGATCAA TTCCACAGTT TTCGCGATCC AGACTGAATG CCCACAGGCC GTCGAGTTTT TTGATTTCAC GGGTTGGGGT TTCTACAGGA CGGACCATAT TTATAGGTGT AATTTATGTA GCTGTAATTT TTACCTTATT AATATTTTTT ACGCTTTGCA TTCGACGACT GAACTCCCAA ATATATGTTT AACTCGTCTT GGTCGTTTGA ATTTTTGTTG CTGTGTTTCC TAATATTTTC CATCACCTTA AATATGTTAT TGTAATCCTC AATGTTGAAC TTGCAATTGG ACACGGCATA GTTTTCCATA GTCGTGTAAA ACATGGTATT GGCTGCATTG TAATACATCC GACTGAGCGG GTACGGATCT ATGTGTTTGA GCAGCCTGTT CAAAAACTCT GCATCGTCGC AAAACGGAAT TTCGTCGACA GATCTGATCA TGGAGATAAT TAAAATGATA ACCATCTCGC AAATAAATAA GTATTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT AGGATCCATG TCATACTACC ACCACCACCA CCACCACGAC TACGACATCC CCACCACTGA GAACCTGTAC TTCCAGGGCG CTATGGACCC CGAATTCGTG TCTATGCCAT CATACCTGAA CAACAACCTG GAGAAGTGCG ACACTTCCAG CTACGACAAC ACCTCTAACA ACATCAAGAC TTCCATCGAG ACCATCAGCA ACGGAATCGA CGACATCCAG GTGGAAAAGA ACATCGAGAA GAACGAAACT TTCGAGGAAA TGAAGAGGAA GAACAGCAAC AACGAGTTCA TCGACTACAA CGAACACATG GTCAAGCTGT CTCCATACTA CACCACTACC AACCACAGAG GCAAGAACAA GAACGACGAC ACTGACGACA ACAGCGACGA CGACAACATT AACAACTACA ACAACAACAA CAACTCCGGC AACAACAACC AGGACAACAC CATCAGCAAC TTCCCAAACA ACATCCTGGA CCTGTCTGAG TACTTCAAGC CTGAAAACTA CCTGAACGAG GAATTCAAGA AGAAGAACTG CAAGATCATC AAGAGGCTGC TGTGGGGCGA ACTGTTCGAG GAGAAGAAGA AGCGCATCCG CAAGGTGTCA CCTTACGGAA AGCTGAAGAC CTGGGACCTG AAGTGCGTCA TCATCAAGGG TGGCGACGAC CTGAGGCAGG AGCTGCTGGC TTCCCAGCTG ATCAAGCAGT TCAAGATCAT CTTCGAGAAC GCCGGACTGC CTCTGTGGCT GCGTCCCTAC GAAATCCTGG TGACTGGATC TAACTCAGGT ATCATCGAGT ACGTGAACGA CACCTGCTCC GTCGACAGCC TGAAGCGCAA GTTCGGTGCT GACTCCATCA GCACTATCTT CAACATCGTG TTCTCAGACT ACATCTTCGA GGCCAAGAAG AACTTCATCG AATCCCACGC TGCCTACTCT CTGATCTCAT ACCTGCTGCA GGTCAAGGAC CGTCACAACG GCAACCTGCT GCTGGACAGC GACGGACACC TGATCCACAT CGACTACGGT TTCATGCTGA CCAACTCCCC TGGCAACGTC AACTTCGAGA CTAGCCCCTT CAAGCTGACC CAGGAATACC TGGACATCAT GGACGGAGAG AAGTCTGACA ACTACGAATA CTTCCGCCGT CTGATCGTGT CTGGTTTCCT GGAAGCCCGC AAGCACTCAG AGGAAATCAT CCTGTTCGTC GAGCTGATGA TGCCAGCTCT GAAGATCCCT TGCTTCGCCA ACGGTACTCA GTTCTGCATC GAGTCCCTGA AGGAAAGGTT CATGACTAAC CTGACCGTGG ACGTCTGCAT CCAGAGAATC AACGCTCTGA TCGAGGCCTC TATCAACAAC TTCCGCTCAG TCCAGTACGA CTACTTCCAG CGTATCACCA ACGGCATCAT GTGATAAGCT TGCGGCCGCA CTCGAGCACC ACCACCACCA CCACTAACCT AGGTAGCTGA GCGCATGCAA GCTGATCCGG GTTATTAGTA CATTTATTAA GCGCTAGATT CTGTGCGTTG TTGATTTACA GACAATTGTT GTACGTATTT TAATAATTCA TTAAATTTAT AATCTTTAGG GTGGTATGTT AGAGCGAAAA TCAAATGATT TTCAGCGTCT TTATATCTGA ATTTAAATAT TAAATCCTCA ATAGATTTGT AAAATAGGTT TCGATTAGTT TCAAACAAGG GTTGTTTTTC CGAACCGATG GCTGGACTAT CTAATGGATT TTCGCTCAAC GCCACAAAAC TTGCCAAATC TTGTAGCAGC AATCTAGCTT TGTCGATATT CGTTTGTGTT TTGTTTTGTA ATAAAGGTTC GACGTCGTTC AAAATATTAT GCGCTTTTGT ATTTCTTTCA TCACTGTCGT TAGTGTACAA TTGACTCGAC GTAAACACGT TAAATAGAGC TTGGACATAT TTAACATCGG GCGTGTTAGC TTTATTAGGC CGATTATCGT CGTCGTCCCA ACCCTCGTCG TTAGAAGTTG CTTCCGAAGA CGATTTTGCC ATAGCCACAC GACGCCTATT AATTGTGTCG GCTAACACGT CCGCGATCAA ATTTGTAGTT GAGCTTTTTG GAATTATTTC TGATTGCGGG CGTTTTTGGG CGGGTTTCAA TCTAACTGTG CCCGATTTTA ATTCAGACAA CACGTTAGAA AGCGATGGTG CAGGCGGTGG TAACATTTCA GACGGCAAAT CTACTAATGG CGGCGGTGGT GGAGCTGATG ATAAATCTAC CATCGGTGGA GGCGCAGGCG GGGCTGGCGG CGGAGGCGGA GGCGGAGGTG GTGGCGGTGA TGCAGACGGC GGTTTAGGCT CAAATGTCTC TTTAGGCAAC ACAGTCGGCA CCTCAACTAT TGTACTGGTT TCGGGCGCCG TTTTTGGTTT GACCGGTCTG AGACGAGTGC GATTTTTTTC GTTTCTAATA GCTTCCAACA ATTGTTGTCT GTCGTCTAAA GGTGCAGCGG GTTGAGGTTC CGTCGGCATT GGTGGAGCGG GCGGCAATTC AGACATCGAT GGTGGTGGTG GTGGTGGAGG CGCTGGAATG TTAGGCACGG GAGAAGGTGG TGGCGGCGGT GCCGCCGGTA TAATTTGTTC TGGTTTAGTT TGTTCGCGCA CGATTGTGGG CACCGGCGCA GGCGCCGCTG GCTGCACAAC GGAAGGTCGT CTGCTTCGAG GCAGCGCTTG GGGTGGTGGC AATTCAATAT TATAATTGGA ATACAAATCG TAAAAATCTG CTATAAGCAT TGTAATTTCG CTATCGTTTA CCGTGCCGAT ATTTAACAAC CGCTCAATGT AAGCAATTGT ATTGTAAAGA GATTGTCTCA AGCTCGGATC GATCCCGCAC GCCGATAACA AGCCTTTTCA TTTTTACTAC AGCATTGTAG TGGCGAGACA CTTCGCTGTC GTCGAGGTTT AAACGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCA
Details for PlfaA.20309.a.DB21.PD38483
PURIFICATION DATe: 2/7/2022
CONCENTRATION: 0.85mg/ml
OBSERVED MW: 72kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM tris pH 7.5, 5% glycerol, 200mM NaCl, 0.5mM TCEP, 5mM maltose
EXPRESSION HOST: Tni cells
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
CID103823, PID9459-1, PUR3099
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSHHHHHHH HENLYFQGSG SGSKIEEGKL VIWINGDKGY NGLAEVGKKF EKDTGIKVTV EHPDKLEEKF PQVAATGDGP DIIFWAHDRF GGYAQSGLLA EITPAAAFQD KLYPFTWDAV RYNGKLIAYP IAVEALSLIY NKDLLPNPPK TWEEIPALDK ELKAKGKSAL MFNLQEPYFT WPLIAADGGY AFKYAAGKYD IKDVGVDNAG AKAGLTFLVD LIKNKHMNAD TDYSIAEHAF NHGHTAMTIN GPWAWSNIDT SAVNYGVTVL PTFKGQPSKP FVGVLSAGIN AASPNKELAK EFLENYLLTD EGLEAVNKDK PLGAVALKSY EEELAKDPRI AATMENAQKG EIMPNIPQMS AFWYAVRTAV INAASGRQTV DAALAAAQTN AAAGSSGKCV IIKGGDDLRQ ELLASQLIKQ FKIIFENAGL PLWLRPYEIL VTGSNSGIIE YVNDTCSVDS LKRKFGADSI STIFNIVFSD YIFEAKKNFI ESHAAYSLIS YLLQVKDRHN GNLLLDSDGH LIHIDYGFML TNSPGNVNFE TSPFKLTQEY LDIMDGEKSD NYEYFRRLIV SGFLEARKHS EEIILFVELM MPALKIPCFA NGTQFCIESL KERFMTNLTV DVCIQRINAL IEASINNFRS VQYDYFQRIT NGIM
Full NT Sequence (Expression Vector + Insert)
GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA TCAATATATA GTTGCTGATC AGATCCTCAT TGTTTGCCTC CCTGCTGCGG TTTTTCACCG AAGTTCATGC CAGTCCAGCG TTTTTGCAGC AGAAAAGCCG CCGACTTCGG TTTGCGGTCG CGAGTGAAGA TCCCTTTCTT GTTACCGCCA ACGCGCAATA TGCCTTGCGA GGTCGCAAAA TCGGCGAAAT TCCATACCTG TTCACCGACG ACGGCGCTGA CGCGATCAAA GACGCGGTGA TACATATCCA GCCATGCACA CTGATACTCT TCACTCCACA TGTCGGTGTA CATTGAGTGC AGCCCGGCTA ACGTATCCAC GCCGTATTCG GTGATGATAA TCGGCTGATG CAGTTTCTCC TGCCAGGCCA GAAGTTCTTT TTCCAGTACC TTCTCTGCCG TTTCCAAATC GCCGCTTTGG ACATACCATC CGTAATAACG GTTCAGGCAC AGCACATCAA AGAGATCGCT GATGGTATCG GTGTGAGCGT CGCAGAACAT TACATTGACG CAGGTGATCG GACGCGTCGG GTCGAGTTTA CGCGTTGCTT CCGCCAGTGG CGCGAAATAT TCCCGTGCAC CTTGCGGACG GGTATCCGGT TCGTTGGCAA TACTCCACAT CACCACGCTT GGGTGGTTTT TGTCACGCGC TATCAGCTCT TTAATCGCCT GTAAGTGCGC TTGCTGAGTT TCCCCGTTGA CTGCCTCTTC GCTGTACAGT TCTTTCGGCT TGTTGCCCGC TTCGAAACCA ATGCCTAAAG AGAGCGAAAA GCCGACAGCA GCAGTTTCAT CAATCACCAC GATGCCATGT TCATCTGCCC AGTCGAGCAT CTCTTCAGCG TAAGGGTAAT GCGAGGTACG GTAGGAGTTG GCCCCAATCC AGTCCATTAA TGCGTGGTCG TGCACCATCA GCACGTTATC GAATCCTTTG CCACGTAAGT CCGCATCTTC ATGACGACCA AAGCCAGTAA AGTAGAACGG TTTGTGGTTA ATCAGGAACT GTTGGCCCTT CACTGCCACT GACCGGATGC CGACGCGAAG CGGGTAGATA TCACACTCTG TCTGGCTTTT GGCTGTGACG CACAGTTCAT AGAGATAACC TTCACCCGGT TGCCAGAGGT GCGGATTCAC CACTTGCAAA GTCCCGCTAG TGCCTTGTCC AGTTGCAACC ACCTGTTGAT CCGCATCACG CAGTTCAACG CTGACATCAC CATTGGCCAC CACCTGCCAG TCAACAGACG CGTGGTTACA GTCTTGCGCG ACATGCGTCA CCACGGTGAT ATCGTCCACC CAGGTGTTCG GCGTGGTGTA GAGCATTACG CTGCGATGGA TTCCGGCATA GTTAAAGAAA TCATGGAAGT AAGACTGCTT TTTCTTGCCG TTTTCGTCGG TAATCACCAT TCCCGGCGGG ATAGTCTGCC AGTTCAGTTC GTTGTTCACA CAAACGGTGA TACGTACACT TTTCCCGGCA ATAACATACG GCGTGACATC GGCTTCAAAT GGCGTATAGC CGCCCTGATG CTCCATCACT TCCTGATTAT TGACCCACAC TTTGCCGTAA TGAGTGACCG CATCGAAACG CAGCACGATA CGCTGGCCTG CCCAACCTTT CGGTATAAAG ACTTCGCGCT GATACCAGAC GTTGCCCGCA TAATTACGAA TATCTGCATC GGCGAACTGA TCGTTAAAAC TGCCTGGCAC AGCAATTGCC CGGCTTTCTT GTAACGCGCT TTCCCACCAA CGCTGATCAA TTCCACAGTT TTCGCGATCC AGACTGAATG CCCACAGGCC GTCGAGTTTT TTGATTTCAC GGGTTGGGGT TTCTACAGGA CGGACCATAT TTATAGGTGT AATTTATGTA GCTGTAATTT TTACCTTATT AATATTTTTT ACGCTTTGCA TTCGACGACT GAACTCCCAA ATATATGTTT AACTCGTCTT GGTCGTTTGA ATTTTTGTTG CTGTGTTTCC TAATATTTTC CATCACCTTA AATATGTTAT TGTAATCCTC AATGTTGAAC TTGCAATTGG ACACGGCATA GTTTTCCATA GTCGTGTAAA ACATGGTATT GGCTGCATTG TAATACATCC GACTGAGCGG GTACGGATCT ATGTGTTTGA GCAGCCTGTT CAAAAACTCT GCATCGTCGC AAAACGGAAT TTCGTCGACA GATCTGATCA TGGAGATAAT TAAAATGATA ACCATCTCGC AAATAAATAA GTATTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT AGGATCCATG GGCTCCCACC ATCACCACCA TCATCACCAC GAAAACCTGT ACTTCCAAGG GTCCGGCTCG GGTTCCAAGA TTGAGGAAGG GAAGCTCGTG ATCTGGATCA ACGGAGATAA GGGTTACAAT GGGTTGGCCG AGGTCGGAAA GAAGTTCGAA AAGGACACCG GAATCAAGGT CACCGTGGAA CACCCGGACA AGCTGGAAGA GAAGTTCCCT CAAGTGGCCG CTACTGGCGA CGGCCCTGAT ATCATCTTTT GGGCCCACGA CCGGTTCGGA GGATACGCCC AGTCCGGTCT GCTGGCCGAA ATTACCCCAG CCGCTGCGTT CCAAGACAAG CTGTACCCCT TTACCTGGGA TGCCGTGCGC TACAATGGAA AGCTGATTGC CTACCCCATT GCCGTGGAAG CCCTTTCGCT GATCTATAAC AAGGACCTTC TCCCTAACCC GCCTAAGACC TGGGAGGAAA TCCCCGCCCT GGATAAGGAA CTCAAGGCCA AGGGAAAGAG CGCCCTCATG TTCAACCTCC AAGAGCCTTA CTTCACATGG CCGCTGATTG CCGCCGATGG AGGCTACGCC TTCAAATACG CGGCGGGGAA ATACGACATC AAGGACGTGG GCGTGGACAA TGCCGGTGCA AAGGCCGGCC TCACCTTCCT GGTGGACCTG ATCAAGAACA AGCACATGAA CGCCGATACC GACTACTCCA TCGCCGAACA CGCTTTCAAC CACGGCCATA CCGCGATGAC CATTAACGGT CCCTGGGCGT GGTCGAACAT CGACACCTCT GCCGTGAACT ACGGAGTGAC CGTGCTCCCG ACTTTCAAAG GACAGCCCTC CAAGCCGTTC GTGGGAGTGC TGAGCGCCGG CATCAACGCG GCTAGCCCGA ACAAGGAGCT GGCCAAGGAG TTCCTGGAGA ACTATCTCCT GACTGATGAA GGCCTGGAGG CCGTCAACAA GGACAAGCCG CTTGGAGCCG TGGCCCTGAA ATCATACGAG GAGGAGCTGG CGAAGGACCC TCGGATTGCT GCGACTATGG AGAACGCGCA GAAGGGGGAA ATCATGCCCA ACATACCCCA GATGTCGGCC TTTTGGTACG CCGTCAGAAC GGCTGTGATC AACGCAGCCT CCGGAAGGCA GACTGTCGAC GCAGCACTGG CAGCGGCACA GACTAACGCC GCTGCCGGCT CCAGCGGCAA GTGCGTCATC ATCAAGGGAG GTGACGACCT GCGTCAGGAG CTGCTGGCTT CACAGCTGAT CAAGCAGTTC AAGATCATCT TCGAGAACGC CGGTCTGCCT CTGTGGCTGA GGCCCTACGA AATCCTGGTG ACTGGTTCCA ACAGCGGCAT CATCGAGTAC GTGAACGACA CCTGCAGCGT CGACTCTCTG AAGAGAAAGT TCGGCGCTGA CTCTATCTCA ACTATCTTCA ACATCGTGTT CTCCGACTAC ATCTTCGAAG CCAAGAAGAA CTTCATCGAG AGCCACGCTG CCTACTCACT GATCTCCTAC CTGCTGCAGG TCAAGGACCG CCACAACGGA AACCTGCTGC TGGACTCCGA CGGTCACCTG ATCCACATCG ACTACGGCTT CATGCTGACC AACTCACCCG GAAACGTCAA CTTCGAGACT TCCCCATTCA AGCTGACCCA GGAATACCTG GACATCATGG ACGGAGAAAA GAGCGACAAC TACGAGTACT TCCGCCGTCT GATCGTGAGC GGTTTCCTGG AAGCCCGCAA GCACTCTGAG GAAATCATCC TGTTCGTCGA GCTGATGATG CCAGCTCTGA AGATCCCTTG CTTCGCCAAC GGCACCCAGT TCTGCATCGA ATCTCTGAAG GAGAGATTCA TGACTAACCT GACCGTGGAC GTCTGCATCC AGCGCATCAA CGCTCTGATC GAGGCCTCAA TCAACAACTT CCGCTCGGTC CAATACGACT ACTTCCAAAG AATCACTAAC GGCATTATGC TCGAGCACCA CCACCACCAC CACTAACCTA GGTAGCTGAG CGCATGCAAG CTGATCCGGG TTATTAGTAC ATTTATTAAG CGCTAGATTC TGTGCGTTGT TGATTTACAG ACAATTGTTG TACGTATTTT AATAATTCAT TAAATTTATA ATCTTTAGGG TGGTATGTTA GAGCGAAAAT CAAATGATTT TCAGCGTCTT TATATCTGAA TTTAAATATT AAATCCTCAA TAGATTTGTA AAATAGGTTT CGATTAGTTT CAAACAAGGG TTGTTTTTCC GAACCGATGG CTGGACTATC TAATGGATTT TCGCTCAACG CCACAAAACT TGCCAAATCT TGTAGCAGCA ATCTAGCTTT GTCGATATTC GTTTGTGTTT TGTTTTGTAA TAAAGGTTCG ACGTCGTTCA AAATATTATG CGCTTTTGTA TTTCTTTCAT CACTGTCGTT AGTGTACAAT TGACTCGACG TAAACACGTT AAATAGAGCT TGGACATATT TAACATCGGG CGTGTTAGCT TTATTAGGCC GATTATCGTC GTCGTCCCAA CCCTCGTCGT TAGAAGTTGC TTCCGAAGAC GATTTTGCCA TAGCCACACG ACGCCTATTA ATTGTGTCGG CTAACACGTC CGCGATCAAA TTTGTAGTTG AGCTTTTTGG AATTATTTCT GATTGCGGGC GTTTTTGGGC GGGTTTCAAT CTAACTGTGC CCGATTTTAA TTCAGACAAC ACGTTAGAAA GCGATGGTGC AGGCGGTGGT AACATTTCAG ACGGCAAATC TACTAATGGC GGCGGTGGTG GAGCTGATGA TAAATCTACC ATCGGTGGAG GCGCAGGCGG GGCTGGCGGC GGAGGCGGAG GCGGAGGTGG TGGCGGTGAT GCAGACGGCG GTTTAGGCTC AAATGTCTCT TTAGGCAACA CAGTCGGCAC CTCAACTATT GTACTGGTTT CGGGCGCCGT TTTTGGTTTG ACCGGTCTGA GACGAGTGCG ATTTTTTTCG TTTCTAATAG CTTCCAACAA TTGTTGTCTG TCGTCTAAAG GTGCAGCGGG TTGAGGTTCC GTCGGCATTG GTGGAGCGGG CGGCAATTCA GACATCGATG GTGGTGGTGG TGGTGGAGGC GCTGGAATGT TAGGCACGGG AGAAGGTGGT GGCGGCGGTG CCGCCGGTAT AATTTGTTCT GGTTTAGTTT GTTCGCGCAC GATTGTGGGC ACCGGCGCAG GCGCCGCTGG CTGCACAACG GAAGGTCGTC TGCTTCGAGG CAGCGCTTGG GGTGGTGGCA ATTCAATATT ATAATTGGAA TACAAATCGT AAAAATCTGC TATAAGCATT GTAATTTCGC TATCGTTTAC CGTGCCGATA TTTAACAACC GCTCAATGTA AGCAATTGTA TTGTAAAGAG ATTGTCTCAA GCTCGGATCG ATCCCGCACG CCGATAACAA GCCTTTTCAT TTTTACTACA GCATTGTAGT GGCGAGACAC TTCGCTGTCG TCGAGGTTTA AACGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCA