PlfaA.18982.a

P-type ATPase4

CENTER ID: PlfaA.18982.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18982.a.ET11.GE00394 full length 2 1265
PlfaA.18982.a.EU11.GE00395 full length 1 1265
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1211900
UniProt: Q9U445

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSQNNNKQG GQDINNKKDS DDIKPSVSKE DLINSLKNDE LNKNTTMDQN DMKKNENMNI KKNEVLNNSN NVEDGDNENS KFMNKSKEGL NNINGEKNDD NNSIVKVEES PKSIGYNYYA SESIENLCKE FGLESINTGL NSEQVKINRD KYGENFIEKD EVVPVWLIFL SQYCSPVVLL LLVAAVASLA LNEVVEGVAI ISIVTLNACL ATYMEKSSGD AIGKLAEMAS PQCTVLRNGQ KVVIPSREVV VGDVVLINTG DSISADLRLF DVIELKTNES LLTGESEDIK KTIVADNLST PFATNLCFAT TSVTSGSGKG IVISTGLDTQ VGKIASQLKK SSKGSKLTPL QVALNKLGGL IGLIAIIVLV VIISLAVIIK YRDPAHADKD PTFVIIIIGV GFAVSSIPEG LPMVVTITLS AGAKDMVKKN ANVRKLPAVE TLGCCSVICS DKTGTLTEGK MTAINAVTIC KNSSLSDENN KLTKTFDFYP TKGFEPCGGL FDSNELTSEK KKEIVIAKNQ NTSYDKVLYN YGNPSNKSVI VDKTRSLMFA AYLNSYDTTL SRDPKTLKWG IHGNMSEGPI VVAAAKVGYS FINNPNHKSY LDNFQRLDDL EVTFNSSRKM KITFYKLKTV NVFENVYLDK PGKVYTHVAL IKGAPDRLLD RSTHLLEETS MKKVQVSWNS TITQEERNVL IKKNLELSQK ALRVLSICIK PLTDQNIEEL KKLEDADERL KYVNYDENGG FIPMGYVASF DPPRPGVKEA IQTCREAQVK VIMITGDQKP TAVAIGKLIG LIEEKSEQVE DINSLAIECS ELHINKNPNE PILPNDQLDE FTDKILIYSR AQPEDKITIV QSLKRKGYLV AMTGDGVNDA PALKAADIGV AMGINGTEVA KGASEMILID DNFCTVVSAI DVGRTIFSNI QKFVCFLLGT NIGEIIYLSV AIVAQMPFPL EALQILFLNL MTDGCPAVAL SREPPNDDNM KTPPRPKKQP IMTKRWWFYG ILPHTIFEAL CVLLSLAFSL YICTGFYNLN GIHNLCKTVN LVDVNDANVY HEYKYFCSSY EYRISTDYVG WVTNVSFWDP QNNEAVNFWG AAKGKVENIN PLSDIVHPEL RLRMQDGCSG DLTLDENGWC RPKDNKTSDG YNDELEGILK KGFEDVTAKG SKRGRTMAFI SAVWCEMLRA YTVRSWEPFY KVFNRNMWMH LACSISATLT FLSTCIPGIT SILNTTCLLW WQYLLAIFWA LLNLFLDEIV PKVIYRRKYM TIKN
NT Sequence
ATGAGTTCTC AAAATAATAA TAAACAGGGT GGACAAGATA TTAATAATAA AAAAGACAGT GATGACATAA AACCTTCTGT AAGCAAAGAA GATTTAATAA ATTCATTAAA AAATGATGAA TTAAATAAAA ATACAACGAT GGATCAAAAC GACATGAAAA AAAATGAAAA TATGAATATT AAGAAAAATG AAGTTTTAAA TAATTCCAAT AATGTAGAAG ACGGAGATAA TGAAAATTCG AAATTTATGA ATAAAAGTAA AGAAGGATTG AATAATATAA ATGGAGAAAA GAATGATGAC AATAATTCTA TTGTAAAAGT AGAAGAATCA CCTAAATCTA TAGGATATAA TTATTATGCA TCTGAAAGTA TTGAAAATTT ATGTAAAGAA TTTGGTTTAG AAAGTATAAA TACTGGTTTA AATTCTGAAC AAGTAAAAAT AAATAGAGAC AAATATGGAG AAAACTTTAT TGAAAAAGAT GAAGTGGTAC CTGTATGGTT AATATTTTTA TCACAATATT GTAGTCCTGT TGTTTTGTTA TTGTTAGTTG CTGCTGTAGC TAGTCTTGCA TTAAATGAAG TTGTTGAAGG AGTCGCTATT ATAAGTATAG TTACCTTGAA TGCTTGCTTA GCAACCTATA TGGAAAAATC ATCAGGTGAT GCTATAGGCA AATTAGCTGA AATGGCTTCA CCACAATGTA CTGTGTTAAG AAATGGTCAG AAGGTAGTTA TACCGTCTCG AGAAGTTGTT GTTGGTGATG TTGTTCTTAT TAATACTGGT GATTCTATTT CAGCTGATTT GAGATTATTT GATGTTATTG AACTTAAAAC GAATGAAAGT TTATTAACAG GAGAATCAGA AGATATTAAA AAAACCATTG TAGCAGATAA TTTGTCTACA CCTTTTGCAA CGAATTTATG TTTTGCTACC ACATCTGTAA CTAGCGGTTC TGGTAAAGGT ATTGTTATAT CCACAGGTTT AGATACACAA GTAGGAAAAA TTGCATCCCA ATTAAAAAAA AGCAGTAAAG GAAGTAAATT AACACCTCTT CAAGTAGCAT TAAATAAATT AGGTGGTTTA ATTGGTTTAA TAGCTATTAT TGTATTAGTT GTTATTATCA GCTTAGCTGT TATTATTAAA TATAGAGATC CAGCACATGC AGATAAAGAT CCAACCTTTG TTATTATTAT TATTGGTGTA GGTTTTGCTG TATCTTCCAT TCCAGAAGGT TTACCTATGG TTGTTACTAT CACCTTATCA GCAGGAGCTA AAGATATGGT TAAAAAAAAT GCAAATGTAA GAAAACTACC AGCTGTTGAA ACTTTAGGAT GCTGTTCAGT CATATGTTCT GATAAAACCG GTACATTAAC TGAAGGAAAA ATGACAGCAA TTAATGCAGT TACCATTTGT AAAAATTCTT CCTTAAGTGA TGAAAATAAC AAATTAACGA AAACATTTGA TTTTTATCCA ACAAAAGGTT TTGAACCATG TGGTGGTTTA TTTGATTCTA ATGAATTAAC TAGCGAAAAA AAGAAAGAGA TTGTGATAGC AAAGAATCAA AACACATCAT ATGATAAAGT TTTGTATAAT TATGGTAATC CTTCAAACAA ATCTGTTATT GTAGACAAAA CGAGGTCATT AATGTTTGCT GCTTATTTAA ATTCATATGA TACTACATTA TCAAGAGATC CCAAAACTTT AAAATGGGGA ATACATGGTA ATATGAGTGA AGGACCAATT GTTGTTGCTG CTGCTAAAGT AGGATATAGT TTTATTAATA ATCCAAATCA CAAATCTTAT TTGGATAATT TTCAAAGATT AGATGATTTA GAAGTTACAT TTAATTCATC AAGAAAAATG AAAATCACTT TTTATAAATT AAAAACAGTA AATGTTTTCG AGAATGTATA TTTAGATAAA CCTGGTAAAG TATATACACA TGTTGCTTTG ATCAAAGGTG CACCTGATAG ATTACTTGAT AGAAGTACCC ATTTATTAGA AGAAACATCT ATGAAAAAAG TACAAGTTTC ATGGAATAGT ACAATAACAC AAGAAGAAAG AAATGTATTA ATAAAAAAGA ATTTGGAATT ATCACAAAAA GCTTTAAGAG TTTTATCCAT ATGTATTAAG CCACTAACGG ATCAAAATAT TGAAGAATTG AAAAAATTAG AAGATGCAGA TGAAAGATTA AAATATGTTA ATTATGATGA AAATGGTGGT TTTATACCTA TGGGTTATGT TGCATCATTT GATCCCCCAA GACCTGGAGT AAAAGAAGCT ATACAAACAT GTAGAGAAGC ACAAGTTAAA GTTATTATGA TTACAGGGGA TCAAAAACCT ACTGCGGTTG CTATTGGAAA ATTAATAGGT TTGATAGAAG AGAAATCTGA ACAAGTAGAA GATATTAATT CTTTAGCTAT AGAATGTTCA GAATTACATA TTAATAAAAA TCCAAATGAA CCAATTTTAC CAAATGATCA ATTAGATGAA TTTACTGATA AAATTTTAAT ATATTCAAGA GCACAACCGG AAGATAAAAT AACAATTGTT CAATCTTTAA AGAGAAAAGG ATATTTAGTT GCTATGACAG GAGATGGAGT TAATGATGCA CCTGCATTGA AGGCTGCAGA TATAGGTGTT GCTATGGGTA TTAATGGAAC AGAAGTAGCT AAAGGAGCAT CTGAAATGAT TTTAATTGAT GACAACTTTT GTACAGTTGT TAGTGCAATA GATGTAGGAA GAACTATTTT CTCAAATATT CAAAAATTTG TATGTTTCTT GCTTGGAACA AATATTGGTG AAATAATTTA CTTATCTGTT GCTATTGTTG CACAAATGCC TTTTCCATTA GAAGCCTTAC AAATATTGTT TTTGAATTTA ATGACAGATG GATGTCCAGC TGTTGCCTTA TCAAGAGAAC CACCAAATGA TGATAATATG AAAACTCCAC CTAGACCTAA AAAGCAACCA ATTATGACGA AACGTTGGTG GTTTTATGGA ATTTTACCAC ATACCATTTT TGAAGCATTA TGTGTCTTGT TATCATTGGC TTTTTCATTG TATATATGTA CAGGTTTTTA TAACTTGAAT GGCATTCACA ATTTGTGTAA AACTGTTAAT TTGGTAGATG TAAATGATGC AAATGTATAC CATGAGTATA AATATTTCTG TAGTAGTTAT GAATATAGGA TATCTACCGA TTATGTTGGA TGGGTAACGA ATGTTAGTTT TTGGGATCCA CAAAATAATG AAGCAGTTAA CTTTTGGGGA GCAGCTAAAG GAAAAGTAGA AAACATTAAT CCATTATCTG ATATTGTCCA CCCAGAATTA AGATTAAGAA TGCAAGATGG TTGTAGTGGT GATTTAACAC TTGATGAAAA TGGATGGTGT AGGCCAAAGG ATAATAAAAC GTCTGATGGA TACAATGATG AACTTGAGGG AATATTAAAA AAAGGTTTTG AAGATGTCAC GGCAAAAGGT TCAAAACGTG GTAGAACAAT GGCATTTATA TCAGCTGTTT GGTGTGAAAT GCTTAGAGCT TATACAGTAA GAAGTTGGGA ACCTTTCTAT AAAGTATTTA ATAGAAACAT GTGGATGCAT TTAGCATGTA GTATATCTGC AACTTTAACA TTTCTTTCAA CATGTATACC TGGTATTACT TCTATTTTGA ATACAACATG TTTGTTATGG TGGCAATATT TATTAGCTAT ATTTTGGGCT CTTTTAAATT TATTCTTAGA TGAAATAGTA CCAAAGGTTA TATATAGAAG AAAATATATG ACTATTAAGA ATTAA
Details for PlfaA.18982.a.ET11.GE00394
HARVESTED ON: 2/3/2015
SEQUENCED ON: 5/4/2017
EXPECTED MW: 143kDa
OBSERVED MW: 143kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: failed sequencing
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MSSQNNNKQG GQDINNKKDS DDIKPSVSKE DLINSLKNDE LNKNTTMDQN DMKKNENMNI KKNEVLNNSN NVEDGDNENS KFMNKSKEGL NNINGEKNDD NNSIVKVEES PKSIGYNYYA SESIENLCKE FGLESINTGL NSEQVKINRD KYGENFIEKD EVVPVWLIFL SQYCSPVVLL LLVAAVASLA LNEVVEGVAI ISIVTLNACL ATYMEKSSGD AIGKLAEMAS PQCTVLRNGQ KVVIPSREVV VGDVVLINTG DSISADLRLF DVIELKTNES LLTGESEDIK KTIVADNLST PFATNLCFAT TSVTSGSGKG IVISTGLDTQ VGKIASQLKK SSKGSKLTPL QVALNKLGGL IGLIAIIVLV VIISLAVIIK YRDPAHADKD PTFVIIIIGV GFAVSSIPEG LPMVVTITLS AGAKDMVKKN ANVRKLPAVE TLGCCSVICS DKTGTLTEGK MTAINAVTIC KNSSLSDENN KLTKTFDFYP TKGFEPCGGL FDSNELTSEK KKEIVIAKNQ NTSYDKVLYN YGNPSNKSVI VDKTRSLMFA AYLNSYDTTL SRDPKTLKWG IHGNMSEGPI VVAAAKVGYS FINNPNHKSY LDNFQRLDDL EVTFNSSRKM KITFYKLKTV NVFENVYLDK PGKVYTHVAL IKGAPDRLLD RSTHLLEETS MKKVQVSWNS TITQEERNVL IKKNLELSQK ALRVLSICIK PLTDQNIEEL KKLEDADERL KYVNYDENGG FIPMGYVASF DPPRPGVKEA IQTCREAQVK VIMITGDQKP TAVAIGKLIG LIEEKSEQVE DINSLAIECS ELHINKNPNE PILPNDQLDE FTDKILIYSR AQPEDKITIV QSLKRKGYLV AMTGDGVNDA PALKAADIGV AMGINGTEVA KGASEMILID DNFCTVVSAI DVGRTIFSNI QKFVCFLLGT NIGEIIYLSV AIVAQMPFPL EALQILFLNL MTDGCPAVAL SREPPNDDNM KTPPRPKKQP IMTKRWWFYG ILPHTIFEAL CVLLSLAFSL YICTGFYNLN GIHNLCKTVN LVDVNDANVY HEYKYFCSSY EYRISTDYVG WVTNVSFWDP QNNEAVNFWG AAKGKVENIN PLSDIVHPEL RLRMQDGCSG DLTLDENGWC RPKDNKTSDG YNDELEGILK KGFEDVTAKG SKRGRTMAFI SAVWCEMLRA YTVRSWEPFY KVFNRNMWMH LACSISATLT FLSTCIPGIT SILNTTCLLW WQYLLAIFWA LLNLFLDEIV PKVIYRRKYM TIKNKLENLY FQGHHHHHHH HHH
Full NT Sequence (Expression Vector + Insert)
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aacttttagg aggtaaaaca tatgagcagc cagaataaca acaaacaggg cggtcaagac atcaacaaca aaaaagacag cgacgacatt aaaccgtccg tgtcaaaaga ggacctgatt aactctctga aaaatgacga gctgaataag aacaccacca tggaccagaa tgatatgaaa aagaacgaaa acatgaatat caagaaaaac gaagtgttga acaatagcaa taatgtcgaa gatggcgata atgaaaacag caaattcatg aataagagca aagagggtct gaataacatt aacggcgaga aaaacgacga taacaattcc atcgttaaag ttgaagagag cccgaaaagc attggttaca attactatgc gagcgagagc atcgagaatc tgtgcaaaga atttggcctg gagagcatta acacgggtct gaacagcgag caagtgaaaa tcaaccgcga taagtacggt gagaatttca tcgagaaaga cgaagtcgtc ccggtttggc tgatcttcct gagccaatat tgcagcccgg ttgtgctgct gctgttggtc gcggctgtcg cctctctggc gttgaatgaa gttgtcgagg gtgtcgcaat catttctatc gtcaccctga acgcgtgctt ggcgacctat atggagaagt ccagcggtga tgccatcggt aaactggccg agatggcctc cccgcagtgt accgtcctgc gtaatggcca aaaagttgtt atcccatccc gcgaagttgt tgtgggtgat gtcgtactga ttaacaccgg tgacagcatc agcgcggatc tgcgtctgtt tgatgttatc gagctgaaaa ccaacgaaag cctgctgacc ggtgagtctg aggacatcaa aaagaccatc gttgcggata acctgagcac tccgttcgca acgaacctgt gctttgctac cacctccgtc acgagcggta gcggcaaggg tatcgtgatt agcacgggtc tggacaccca ggtcggtaag attgcgagcc agttaaagaa atctagcaag ggttctaagc tgaccccgct gcaagtggcg ttgaacaaac tgggtggtct gatcggtctg attgcgatta ttgtgctggt tgtcattatc agcctggcag tgatcattaa gtatcgcgac ccggcccacg ctgataagga cccgactttt gtgatcatca ttattggcgt cggctttgca gtgagcagca tcccggaagg cctgccgatg gttgtgacga ttacgctgtc cgccggtgcg aaagacatgg ttaagaaaaa tgcgaacgtc cgtaagctgc cggcagttga gactctgggt tgctgcagcg tgatttgcag cgataaaacg ggcaccctga ccgaaggcaa aatgaccgct atcaatgccg tcacgatttg caagaatagc agcctgagcg acgagaacaa taagctgacc aaaaccttcg acttctatcc gaccaagggt tttgagccgt gtggcggcct gtttgacagc aacgagttga cgagcgagaa gaaaaaagag attgttatcg caaagaatca aaataccagc tatgacaaag ttctgtacaa ctacggcaac ccgagcaata agagcgtcat cgtagacaag actcgttcgc tgatgtttgc ggcgtacctg aatagctacg ataccacgct gagccgtgat ccgaaaacgc tgaagtgggg tatccacggc aatatgagcg aaggccctat tgttgtggcc gctgcaaagg ttggctattc gttcatcaac aacccgaacc ataaaagcta cctggacaat ttccagcgtc tggacgacct ggaagtgacg tttaacagct cgcgcaagat gaagatcacg ttctacaagc tgaaaactgt taatgtgttt gaaaacgtgt atttggacaa gccaggtaaa gtgtataccc acgtggcgct gattaagggc gcaccggatc gcctgttgga ccgtagcacc cacttgttgg aagaaaccag catgaaaaaa gtccaagtgt catggaactc taccatcacc caagaagaac gtaatgtgct gatcaaaaag aatctggaac tgagccagaa agcgctgcgt gttctgagca tttgtattaa gccgctgacc gaccagaata ttgaagaatt gaagaaactg gaagatgctg atgagcgtct taagtacgtc aactatgacg aaaatggtgg ctttattccg atgggttacg ttgcgtcttt cgatcctccg cgtccgggtg ttaaagaggc aatccagacc tgtcgtgagg cacaagttaa agtgattatg atcaccggcg atcagaagcc gaccgcggtt gctatcggta agctgattgg tctgattgaa gagaagtccg agcaagtgga agatatcaat agcctggcga ttgagtgttc cgagctgcac attaacaaga atccgaatga acctatcctg ccgaatgacc agctggatga atttaccgac aagattctga tttactctcg cgcgcaacca gaggataaga ttacgattgt gcagagcctg aaacgtaagg gctatctggt tgcaatgacc ggcgatggtg tgaacgatgc accggcactg aaagctgcgg atatcggtgt ggctatgggt attaacggta ccgaagtggc gaagggcgcg agcgagatga ttctgatcga tgacaatttc tgcaccgtgg tgagcgcgat cgatgtcggc cgtaccattt tcagcaatat ccaaaagttc gtgtgctttc tgttgggtac gaatatcggt gagattatct acctgtccgt cgcgattgtt gcacaaatgc cgtttcctct ggaagccctg cagatcctgt ttctgaattt gatgaccgac ggctgtccgg cagtggccct gtcgcgcgag ccgccgaatg acgacaatat gaaaacccca ccgcgtccga aaaagcaacc gatcatgact aagcgttggt ggttctacgg tattctgccg cataccatct tcgaggcatt gtgtgttctg ctgagcctgg catttagcct gtatatctgc acgggtttct acaatctgaa cggcatccac aatctgtgta aaaccgttaa cttagttgat gtgaatgacg caaacgtgta ccacgagtat aagtacttct gtagctccta cgagtaccgt atttctaccg actacgttgg ttgggtcacc aatgtctcct tttgggaccc acagaacaac gaagccgtga acttctgggg cgctgccaaa ggcaaagtcg agaacattaa cccgttgagc gatattgtcc atccagagct gagactgcgc atgcaagatg gttgtagcgg tgatctgacg ctggacgaga acggctggtg ccgtccgaaa gacaataaga cgagcgatgg ttacaacgac gagctcgaag gcattctgaa gaaaggtttt gaagatgtta ccgcgaaggg tagcaagcgt ggccgcacga tggcgtttat cagcgcggtt tggtgcgaga tgctgcgcgc gtatacggtc cgtagctggg agccgttcta taaagtgttc aaccgtaaca tgtggatgca cctggcttgc tcgatcagcg caaccctcac gttcctgagc acttgcattc cgggtatcac gagcattttg aacaccactt gcctgctgtg gtggcagtat ttgctggcga tcttctgggc gttgctgaat ctgttcttgg atgagattgt gccaaaagtc atctaccgcc gtaagtatat gacgattaag aacaagcttg agaatttgta cttccagggt catcaccatc atcatcacca ccaccatcac tgataactcg agccccctag cataacccct tggggcctct aaacgggtct tgaggggttt tttgcccctg agacgcgtca atcgagttcg tacctaaggg cgacaccccc taattagccc gggcgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgca tggggagtcc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga ccaccgcgct actgccgcca ggcaaacaag gggtgttatg agccatattc aggtataaat gggctcgcga taatgttcag aattggttaa ttggttgtaa cactgacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gaatatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgccact tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcgttcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcactcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gcctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagcggcg cgccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatatga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgagga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagat agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgccagtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtga
Details for PlfaA.18982.a.EU11.GE00395
HARVESTED ON: 2/3/2015
SEQUENCED ON: 5/4/2017
EXPECTED MW: 143kDa
OBSERVED MW: 143kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MGSSHHHHHH SSGENLYFQG SMSSQNNNKQ GGQDINNKKD SDDIKPSVSK EDLINSLKND ELNKNTTMDQ NDMKKNENMN IKKNEVLNNS NNVEDGDNEN SKFMNKSKEG LNNINGEKND DNNSIVKVEE SPKSIGYNYY ASESIENLCK EFGLESINTG LNSEQVKINR DKYGENFIEK DEVVPVWLIF LSQYCSPVVL LLLVAAVASL ALNEVVEGVA IISIVTLNAC LATYMEKSSG DAIGKLAEMA SPQCTVLRNG QKVVIPSREV VVGDVVLINT GDSISADLRL FDVIELKTNE SLLTGESEDI KKTIVADNLS TPFATNLCFA TTSVTSGSGK GIVISTGLDT QVGKIASQLK KSSKGSKLTP LQVALNKLGG LIGLIAIIVL VVIISLAVII KYRDPAHADK DPTFVIIIIG VGFAVSSIPE GLPMVVTITL SAGAKDMVKK NANVRKLPAV ETLGCCSVIC SDKTGTLTEG KMTAINAVTI CKNSSLSDEN NKLTKTFDFY PTKGFEPCGG LFDSNELTSE KKKEIVIAKN QNTSYDKVLY NYGNPSNKSV IVDKTRSLMF AAYLNSYDTT LSRDPKTLKW GIHGNMSEGP IVVAAAKVGY SFINNPNHKS YLDNFQRLDD LEVTFNSSRK MKITFYKLKT VNVFENVYLD KPGKVYTHVA LIKGAPDRLL DRSTHLLEET SMKKVQVSWN STITQEERNV LIKKNLELSQ KALRVLSICI KPLTDQNIEE LKKLEDADER LKYVNYDENG GFIPMGYVAS FDPPRPGVKE AIQTCREAQV KVIMITGDQK PTAVAIGKLI GLIEEKSEQV EDINSLAIEC SELHINKNPN EPILPNDQLD EFTDKILIYS RAQPEDKITI VQSLKRKGYL VAMTGDGVND APALKAADIG VAMGINGTEV AKGASEMILI DDNFCTVVSA IDVGRTIFSN IQKFVCFLLG TNIGEIIYLS VAIVAQMPFP LEALQILFLN LMTDGCPAVA LSREPPNDDN MKTPPRPKKQ PIMTKRWWFY GILPHTIFEA LCVLLSLAFS LYICTGFYNL NGIHNLCKTV NLVDVNDANV YHEYKYFCSS YEYRISTDYV GWVTNVSFWD PQNNEAVNFW GAAKGKVENI NPLSDIVHPE LRLRMQDGCS GDLTLDENGW CRPKDNKTSD GYNDELEGIL KKGFEDVTAK GSKRGRTMAF ISAVWCEMLR AYTVRSWEPF YKVFNRNMWM HLACSISATL TFLSTCIPGI TSILNTTCLL WWQYLLAIFW ALLNLFLDEI VPKVIYRRKY MTIKN
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcg agaaccttta tttccaagga tccatgagca gccagaataa caacaaacag ggcggtcaag acatcaacaa caaaaaagac agcgacgaca ttaaaccgtc cgtgtcaaaa gaggacctga ttaactctct gaaaaatgac gagctgaata agaacaccac catggaccag aatgatatga aaaagaacga aaacatgaat atcaagaaaa acgaagtgtt gaacaatagc aataatgtcg aagatggcga taatgaaaac agcaaattca tgaataagag caaagagggt ctgaataaca ttaacggcga gaaaaacgac gataacaatt ccatcgttaa agttgaagag agcccgaaaa gcattggtta caattactat gcgagcgaga gcatcgagaa tctgtgcaaa gaatttggcc tggagagcat taacacgggt ctgaacagcg agcaagtgaa aatcaaccgc gataagtacg gtgagaattt catcgagaaa gacgaagtcg tcccggtttg gctgatcttc ctgagccaat attgcagccc ggttgtgctg ctgctgttgg tcgcggctgt cgcctctctg gcgttgaatg aagttgtcga gggtgtcgca atcatttcta tcgtcaccct gaacgcgtgc ttggcgacct atatggagaa gtccagcggt gatgccatcg gtaaactggc cgagatggcc tccccgcagt gtaccgtcct gcgtaatggc caaaaagttg ttatcccatc ccgcgaagtt gttgtgggtg atgtcgtact gattaacacc ggtgacagca tcagcgcgga tctgcgtctg tttgatgtta tcgagctgaa aaccaacgaa agcctgctga ccggtgagtc tgaggacatc aaaaagacca tcgttgcgga taacctgagc actccgttcg caacgaacct gtgctttgct accacctccg tcacgagcgg tagcggcaag ggtatcgtga ttagcacggg tctggacacc caggtcggta agattgcgag ccagttaaag aaatctagca agggttctaa gctgaccccg ctgcaagtgg cgttgaacaa actgggtggt ctgatcggtc tgattgcgat tattgtgctg gttgtcatta tcagcctggc agtgatcatt aagtatcgcg acccggccca cgctgataag gacccgactt ttgtgatcat cattattggc gtcggctttg cagtgagcag catcccggaa ggcctgccga tggttgtgac gattacgctg tccgccggtg cgaaagacat ggttaagaaa aatgcgaacg tccgtaagct gccggcagtt gagactctgg gttgctgcag cgtgatttgc agcgataaaa cgggcaccct gaccgaaggc aaaatgaccg ctatcaatgc cgtcacgatt tgcaagaata gcagcctgag cgacgagaac aataagctga ccaaaacctt cgacttctat ccgaccaagg gttttgagcc gtgtggcggc ctgtttgaca gcaacgagtt gacgagcgag aagaaaaaag agattgttat cgcaaagaat caaaatacca gctatgacaa agttctgtac aactacggca acccgagcaa taagagcgtc atcgtagaca agactcgttc gctgatgttt gcggcgtacc tgaatagcta cgataccacg ctgagccgtg atccgaaaac gctgaagtgg ggtatccacg gcaatatgag cgaaggccct attgttgtgg ccgctgcaaa ggttggctat tcgttcatca acaacccgaa ccataaaagc tacctggaca atttccagcg tctggacgac ctggaagtga cgtttaacag ctcgcgcaag atgaagatca cgttctacaa gctgaaaact gttaatgtgt ttgaaaacgt gtatttggac aagccaggta aagtgtatac ccacgtggcg ctgattaagg gcgcaccgga tcgcctgttg gaccgtagca cccacttgtt ggaagaaacc agcatgaaaa aagtccaagt gtcatggaac tctaccatca cccaagaaga acgtaatgtg ctgatcaaaa agaatctgga actgagccag aaagcgctgc gtgttctgag catttgtatt aagccgctga ccgaccagaa tattgaagaa ttgaagaaac tggaagatgc tgatgagcgt cttaagtacg tcaactatga cgaaaatggt ggctttattc cgatgggtta cgttgcgtct ttcgatcctc cgcgtccggg tgttaaagag gcaatccaga cctgtcgtga ggcacaagtt aaagtgatta tgatcaccgg cgatcagaag ccgaccgcgg ttgctatcgg taagctgatt ggtctgattg aagagaagtc cgagcaagtg gaagatatca atagcctggc gattgagtgt tccgagctgc acattaacaa gaatccgaat gaacctatcc tgccgaatga ccagctggat gaatttaccg acaagattct gatttactct cgcgcgcaac cagaggataa gattacgatt gtgcagagcc tgaaacgtaa gggctatctg gttgcaatga ccggcgatgg tgtgaacgat gcaccggcac tgaaagctgc ggatatcggt gtggctatgg gtattaacgg taccgaagtg gcgaagggcg cgagcgagat gattctgatc gatgacaatt tctgcaccgt ggtgagcgcg atcgatgtcg gccgtaccat tttcagcaat atccaaaagt tcgtgtgctt tctgttgggt acgaatatcg gtgagattat ctacctgtcc gtcgcgattg ttgcacaaat gccgtttcct ctggaagccc tgcagatcct gtttctgaat ttgatgaccg acggctgtcc ggcagtggcc ctgtcgcgcg agccgccgaa tgacgacaat atgaaaaccc caccgcgtcc gaaaaagcaa ccgatcatga ctaagcgttg gtggttctac ggtattctgc cgcataccat cttcgaggca ttgtgtgttc tgctgagcct ggcatttagc ctgtatatct gcacgggttt ctacaatctg aacggcatcc acaatctgtg taaaaccgtt aacttagttg atgtgaatga cgcaaacgtg taccacgagt ataagtactt ctgtagctcc tacgagtacc gtatttctac cgactacgtt ggttgggtca ccaatgtctc cttttgggac ccacagaaca acgaagccgt gaacttctgg ggcgctgcca aaggcaaagt cgagaacatt aacccgttga gcgatattgt ccatccagag ctgagactgc gcatgcaaga tggttgtagc ggtgatctga cgctggacga gaacggctgg tgccgtccga aagacaataa gacgagcgat ggttacaacg acgagctcga aggcattctg aagaaaggtt ttgaagatgt taccgcgaag ggtagcaagc gtggccgcac gatggcgttt atcagcgcgg tttggtgcga gatgctgcgc gcgtatacgg tccgtagctg ggagccgttc tataaagtgt tcaaccgtaa catgtggatg cacctggctt gctcgatcag cgcaaccctc acgttcctga gcacttgcat tccgggtatc acgagcattt tgaacaccac ttgcctgctg tggtggcagt atttgctggc gatcttctgg gcgttgctga atctgttctt ggatgagatt gtgccaaaag tcatctaccg ccgtaagtat atgacgatta agaactgata agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat