PlfaA.18794.a

Rhomboid protease ROM4

CENTER ID: PlfaA.18794.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18794.a.B4.GE37863 soluble domain 3 600 680
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_0506900
RefSeq: XP_001351627.1
UniProt: Q8I433

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSNTGFNPK NAEKKRSSIF SDLEIPQGDV KKKASIFNNM EKKKMTIFKK TKGENNEEKK KRSIFNNNNN NNNNNNTMKI NIFNNNNEKK ESFSINGDEK KKSFSINGDE KKESFSINGD EKKESFLINK DEKKESFLIN KDEKEKSFSI NGDEKKESFS INGDEKKESF SINEDETKKS CSINDDERKI SIFSNNEKKK NSIYSDTQSS KREDEKRISI FSDLETSTNI DDISSKRNSQ KLSVYGDNKL KKGSLLSPKV DNYRNTIDNI NEINDIKIIV TSDENLHTLP SGAVGRRAPL NPFSSPILGK YRRKNKNAKA KVKDPRLNNN PLIGRLTVCI STTAILFWVF FAEMVFNYNT FNGRCISKVL YPIYTENVVL KRQPFFVFLG YGACEYNLDE SASNRHFIGS KASDEGWPGD KVEENPDGRG YANWDSVNNR VYNLLGGLNT NYIRNYGELY RLFWSMYLHG GFMHILFNVI CQIQILWMIE PDWGSIRTGL LFFISGVTGN LLSAVCDPCG VTIGSSGSLY GLIGALFAYY IEYWKTIPRP CCVLIFMFLV VMFGIIVGMF GYTDNYAHIG GCLGGVLFGF STITTVSAAD KCTLGERMLV SAPFSWFLSN ETKELIIAKA KDKKIKGENF RKKQLANKVH KNDALHVAMA VMKNRINDEG RPPCRMKLRE WIVRITAAST LIIMWIVLFI YLLNEKAYKS YSPLGQIKFS GVHSCYCCQI VKNKFTYIKV NDFYWCFTTE EATRYYCNK
NT Sequence
atgggatcta atacagggtt taatccgaaa aatgccgaga aaaaaagaag cagcatattt agcgatttag aaattccgca gggtgatgtg aagaaaaaag ctagtatatt caataatatg gaaaagaaga aaatgactat attcaagaag acaaagggtg aaaataatga agagaaaaaa aaaagaagca tatttaataa taataacaat aataataata ataataatac aatgaaaatt aacatattta ataataataa tgaaaaaaaa gaaagctttt caattaatgg agatgaaaag aaaaaaagct tttccattaa tggagatgaa aaaaaagaaa gcttttccat taatggagat gaaaaaaaag aaagcttttt aattaacaaa gatgaaaaaa aagaaagctt tttaattaac aaagatgaaa aagaaaaaag cttttccatt aatggagatg aaaaaaaaga aagcttttcc attaatggag atgaaaaaaa agaaagcttt tccattaatg aagatgaaac aaaaaagagc tgttcaatta atgacgatga aaggaaaatt agtatattca gtaataatga aaaaaaaaaa aacagcatat atagtgatac gcaatcttct aagagagaag atgaaaaaag aattagtata tttagtgact tagaaacatc aactaatata gatgatatat cttccaagag gaattcacaa aaattatcag tatatggaga taataaatta aaaaaaggtt ctttattaag tcctaaagtg gataattata ggaatacaat tgataatata aatgaaataa atgatataaa aattattgta acaagtgatg agaatttaca tacgctacca tctggagctg ttggtagacg tgctccttta aatccttttt catctcctat tcttggaaaa tatagaagaa agaataaaaa tgcaaaagca aaagtcaaag atccaagatt aaacaacaat cctttgatag gcagattaac agtatgtata tcaaccactg ctatattgtt ttgggtattt tttgcggaaa tggtttttaa ttataataca tttaatggga gatgtatatc gaaagtttta taccctattt atactgaaaa tgtggttctt aagaggcagc cgttttttgt attcttagga tatggtgcat gtgaatacaa tttagatgag tctgcatcta atagacattt tattggttct aaagcatccg atgaagggtg gcctggagat aaagtagaag aaaatccaga tggtagagga tatgctaact gggattctgt aaataatcgt gtatataatt tattaggagg tttaaatacc aattatataa gaaattatgg ggaactatat agattatttt ggtctatgta tttacatggt ggattcatgc atattttatt taatgtaatt tgtcaaattc aaatcttatg gatgattgaa cctgattggg gatccattag aacaggtctt ttattcttta tttctggtgt aaccgggaat ttattatcag ctgtttgtga tccttgtggt gttactatag gttcttcggg ttctttatat ggtttaatag gagctttatt tgcgtattat attgaatatt ggaaaacgat tccaagacct tgttgcgttt taatttttat gttccttgta gtaatgtttg gaattattgt aggaatgttt ggatatacag ataattatgc tcacataggg ggttgtctag gtggtgtttt atttggattt tcgactatta caactgtgtc agctgctgat aaatgtacct taggagaaag aatgcttgtg tctgctcctt tctcttggtt tctttcaaat gaaacaaagg aactaataat tgcaaaagca aaagacaaaa aaatcaaagg agaaaatttc agaaagaaac aactagctaa taaagtacat aaaaatgatg ctcttcatgt agctatggct gttatgaaaa atagaataaa tgatgaaggt agacctccat gtcgcatgaa attaagagaa tggatagtac gtatcactgc tgcatcaaca ttaattatta tgtggatagt tttatttatt tatttgttaa atgaaaaggc atacaaatca tattccccat tgggacaaat aaaattctct ggtgtccatt catgttattg ttgtcaaata gtaaagaata aatttactta cattaaggta aatgattttt attggtgttt tacaactgaa gaagcaacaa gatattattg taataagtag
Details for PlfaA.18794.a.B4.GE37863
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 10kDa
OBSERVED MW: 10kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMD KCTLGERMLV SAPFSWFLSN ETKELIIAKA KDKKIKGENF RKKQLANKVH KNDALHVAMA VMKNRINDEG RPPCRMKLRE
Validated NT Sequence
atcttactca cttattctct taatttcatg cgacatggag gtctaccttc atcatttatt ctatttttca taacagccat agctacatga agagcatcat ttttatgtac tttattagct agttgtttct ttctgaaatt ttctcctttg atttttttgt cttttgcttt tgcaattatt agttcctttg tttcatttga aagaaaccaa gagaaaggag cagacacaag cattctttct cctaaggtac atttatccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHDK CTLGERMLVS APFSWFLSNE TKELIIAKAK DKKIKGENFR KKQLANKVHK NDALHVAMAV MKNRINDEGR PPCRMKLRE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgataaat gtaccttagg agaaagaatg cttgtgtctg ctcctttctc ttggtttctt tcaaatgaaa caaaggaact aataattgca aaagcaaaag acaaaaaaat caaaggagaa aatttcagaa agaaacaact agctaataaa gtacataaaa atgatgctct tcatgtagct atggctgtta tgaaaaatag aataaatgat gaaggtagac ctccatgtcg catgaaatta agagaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t