PlfaA.18757.a

Probable cathepsin C (EC 3.4.22.-)

CENTER ID: PlfaA.18757.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18757.a.B2.GE37857 mature protein 27 700
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1116700
RefSeq: XP_001347845.1
UniProt: Q8IIJ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKRIFSVSF LLVLLNVLHI CIKFSVADLP THVETKNLLG KWKILRTKTS PNLTTCGSSQ PNKNTYNVGI TDYKKYLLEN NYEFVSELNV ILSDDYVLYG DIYNTQDNEH RSKWKVLAVY DENKRVIGTW TTICDEGFEI KIGNETYAAL MHYEPNGKCG PVSDEDSLDS NGDTDCYTTS FSKIRYGWLD VENEKNEHLH GCFYAERIFD NVNEIKHLDS FTIDKDSQNV LQTFTYDTKL NNILNSNNML YKFGNLQKPT FTKRNNTNVQ FNSELNWHRM KHHGKKKPLK KSMLDASRQT YACPCNANEV VDNVINKGDS DNPVSPTLIQ LNNNLKNTTQ TGNKDTNEMD LENYEDTLNS PKRELEINEL PKNFTWGDPW NKNTREYEVT NQLLCGSCYI ASQLYAFKRR IEVALTKKLD RKYLNNFDDQ LSIQTVLSCS FYDQGCNGGF PYLVSKLAKL QGIPLNVYFP YSATEETCPY NISKHPNDMN GSAKLREINA IFNSNNNMST YNNINNDHHQ LGVYANTASS QEQHGISEEN RWYAKDFNYV GGCYGCNQCN GEKIMMNEIY RNGPIVSSFE ASPDFYDYAD GVYFVEDFPH ARRCTIEPKN DGVYNITGWD RVNHAIVLLG WGEEEINGKL YKYWIGRNSW GNGWGKEGYF KILRGQNFSG IESQSLFIEP DFSRGAGKIL LEKMQKELGN
NT Sequence
atggcaaaga gaatttttag tgtaagtttt ttactagtat tgttaaacgt tttgcacata tgcattaaat ttagtgttgc tgatttacca acccatgtag aaacaaagaa tttattaggt aaatggaaaa ttttaaggac taaaacatca ccaaatttga ctacttgtgg ttctagtcaa cctaataaga atacatataa tgtaggtata acagattata aaaaatattt gcttgaaaac aattatgagt ttgtttcaga attgaatgta atattatcag atgattatgt attatatggt gatatatata atacccaaga taatgagcac agaagtaaat ggaaagtgtt agctgtgtat gatgagaata agagagttat tggtacatgg acaactatat gtgatgaggg ttttgaaata aaaattggga atgaaacgta tgcagcttta atgcattatg aacctaatgg aaaatgtggt cctgtatctg atgaagattc tttagattca aatggagata ctgattgtta tactacaagt tttagtaaga taagatatgg ttggttagat gtggagaatg aaaaaaatga acatttacat ggttgttttt atgcagaaag aatttttgat aatgtaaatg aaattaaaca tttagattct tttactatag ataaagattc tcaaaatgtt cttcaaacat ttacttatga cactaaatta aataatatct taaattctaa taatatgtta tataaatttg gtaatttaca aaaaccaaca tttaccaaaa gaaacaatac aaatgtacaa tttaattctg aattaaattg gcacagaatg aaacaccacg gaaaaaaaaa accattgaag aaatccatgt tggatgcatc aagacaaact tatgcatgtc catgtaacgc taacgaagtt gttgataatg taataaataa aggagattca gataatccag tatcaccaac cttaattcaa ttaaataata acctaaagaa tacaacacaa acaggtaata aagatacaaa tgaaatggat ttagaaaatt atgaggatac attaaattct cctaaaagag aattagaaat aaacgaatta ccaaagaatt tcacttgggg agacccatgg aataaaaata ccagagaata tgaggttact aatcaattat tatgtggttc atgttatata gcttcacaat tatatgcatt taaaagaaga atagaagtgg cacttaccaa aaaattggat agaaaatatt tgaacaattt tgatgatcaa ttatccatac aaactgtttt atcatgttct ttttatgacc aaggatgtaa tggtggattc ccatatttag tatctaagct agctaaatta caaggtatcc cattaaatgt atatttccca tatagtgcaa ctgaggaaac ttgtccatat aatataagca aacatcctaa tgatatgaat ggttctgcta aattaagaga aatcaatgct atatttaata gtaacaataa tatgagtact tataacaaca ttaataatga ccaccaccaa ttaggtgtat acgcaaatac tgcatcttca caagaacaac atggtatttc tgaagaaaac agatggtacg ctaaagattt taattatgta ggtggttgtt atggatgtaa ccaatgtaat ggtgagaaaa ttatgatgaa tgaaatttat agaaatggtc ctatcgtttc ttcttttgaa gcttctccag atttttatga ctatgctgat ggtgtgtatt ttgttgaaga tttcccacac gctcgtagat gtaccatcga acctaaaaac gatggtgtct ataacataac aggttgggat cgtgttaacc atgccattgt tttgttagga tggggagaag aagaaattaa tggaaaatta tacaaatatt ggataggaag aaatagttgg ggaaatggtt ggggtaaaga aggatacttt aaaattttaa gaggacaaaa tttcagtggt attgaaagtc agagtttgtt tattgaacct gatttttcaa gaggtgctgg taaaatactt ttagaaaaaa tgcaaaagga attaggaaat taa
Details for PlfaA.18757.a.B2.GE37857
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 78kDa
OBSERVED MW: 78kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 45
Validated AA Sequence
MAHHHHHHMA DLPTHVETKN LLGKWKILRT KTSPNLTTCG SSQPNKNTYN VGITDYKKYL LENNYEFVSE LNVILSDDYV LYGDIYNTQD NEHRSKWKVL AVYDENKRVI GTWTTICDEG FEIKIGNETY AALMHYEPNG KCGPVSDEDS LDSNGDTDCY TTSFSKIRYG WLDVENEKNE HLHGCFYAER IFDNVNEIKH LDSFTIDKDS QNVLQTFTYD TKLNNILNSN NMLYKFGNLQ KPTFTKRNNT NVQFNSELNW HRMKHHGKKK PLKKSMLDAS RQTYACPCNA NEVVDNVINK GDSDNPVSPT LIH
Validated NT Sequence
cnctagaann ttttgtttac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg atttaccaac ccatgtagaa acaaagaatt tattaggtaa atggaaaatt ttaaggacta aaacatcacc aaatttgact acttgtggtt ctagtcaacc taataagaat acatataatg taggtataac agattataaa aaatatttgc ttgaaaacaa ttatgagttt gtttcagaat tgaatgtaat attatcagat gattatgtat tatatggtga tatatataat acccaagata atgagcacag aagtaaatgg aaagtgttag ctgtgtatga tgagaataag agagttattg gtacatggac aactatatgt gatgagggtt ttgaaataaa aattgggaat gaaacgtatg cagctttaat gcattatgaa cctaatggaa aatgtggtcc tgtatctgat gaagattctt tagattcaaa tggagatact gattgttata ctacaagttt tagtaagata agatatggtt ggttagatgt ggagaatgaa aaaaatgaac atttacatgg ttgtttttat gcagaaagaa tttttgataa tgtaaatgaa attaaacatt tagattcttt tactatagat aaagattctc aaaatgttct tcaaacattt acttatgaca ctaaattaaa taatatctta aattctaata atatgttata taaatttggt aatttacaaa aaccaacatt taccaaaaga aacaatacaa atgtacaatt taattctgaa ttaaattggc acagaatgaa acaccacgga aaaaaaaaac cattgaagaa atccatgttg gatgcatcaa gacaaactta tgcatgtcca tgtaacgcta acgaagttgt tgataatgta ataaataaag gagattcaga taatccagta tcaccaacct taattcatta aatataacct aaagaataca acacaaacag gtaataaana tacaaatgaa atgnatttag aaaatntgag nnncattaaa ttcnctaaaa gannntagaa anaacgannc nnanttcact tggggagacc catgnntaaa anncnnagaa ntgagnacna ncannantnn ntngnnatgt nnnnagctcc nntnnntgcn tnnnagaaga nnnnnnnnnn nnnaaannnn nanannnnnn nnnnnnnnna tnnnncanac nnnncngntc tttttnncac nng
Expected Protein Sequence
MAHHHHHHAD LPTHVETKNL LGKWKILRTK TSPNLTTCGS SQPNKNTYNV GITDYKKYLL ENNYEFVSEL NVILSDDYVL YGDIYNTQDN EHRSKWKVLA VYDENKRVIG TWTTICDEGF EIKIGNETYA ALMHYEPNGK CGPVSDEDSL DSNGDTDCYT TSFSKIRYGW LDVENEKNEH LHGCFYAERI FDNVNEIKHL DSFTIDKDSQ NVLQTFTYDT KLNNILNSNN MLYKFGNLQK PTFTKRNNTN VQFNSELNWH RMKHHGKKKP LKKSMLDASR QTYACPCNAN EVVDNVINKG DSDNPVSPTL IQLNNNLKNT TQTGNKDTNE MDLENYEDTL NSPKRELEIN ELPKNFTWGD PWNKNTREYE VTNQLLCGSC YIASQLYAFK RRIEVALTKK LDRKYLNNFD DQLSIQTVLS CSFYDQGCNG GFPYLVSKLA KLQGIPLNVY FPYSATEETC PYNISKHPND MNGSAKLREI NAIFNSNNNM STYNNINNDH HQLGVYANTA SSQEQHGISE ENRWYAKDFN YVGGCYGCNQ CNGEKIMMNE IYRNGPIVSS FEASPDFYDY ADGVYFVEDF PHARRCTIEP KNDGVYNITG WDRVNHAIVL LGWGEEEING KLYKYWIGRN SWGNGWGKEG YFKILRGQNF SGIESQSLFI EPDFSRGAGK ILLEKMQKEL GN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgatt taccaaccca tgtagaaaca aagaatttat taggtaaatg gaaaatttta aggactaaaa catcaccaaa tttgactact tgtggttcta gtcaacctaa taagaataca tataatgtag gtataacaga ttataaaaaa tatttgcttg aaaacaatta tgagtttgtt tcagaattga atgtaatatt atcagatgat tatgtattat atggtgatat atataatacc caagataatg agcacagaag taaatggaaa gtgttagctg tgtatgatga gaataagaga gttattggta catggacaac tatatgtgat gagggttttg aaataaaaat tgggaatgaa acgtatgcag ctttaatgca ttatgaacct aatggaaaat gtggtcctgt atctgatgaa gattctttag attcaaatgg agatactgat tgttatacta caagttttag taagataaga tatggttggt tagatgtgga gaatgaaaaa aatgaacatt tacatggttg tttttatgca gaaagaattt ttgataatgt aaatgaaatt aaacatttag attcttttac tatagataaa gattctcaaa atgttcttca aacatttact tatgacacta aattaaataa tatcttaaat tctaataata tgttatataa atttggtaat ttacaaaaac caacatttac caaaagaaac aatacaaatg tacaatttaa ttctgaatta aattggcaca gaatgaaaca ccacggaaaa aaaaaaccat tgaagaaatc catgttggat gcatcaagac aaacttatgc atgtccatgt aacgctaacg aagttgttga taatgtaata aataaaggag attcagataa tccagtatca ccaaccttaa ttcaattaaa taataaccta aagaatacaa cacaaacagg taataaagat acaaatgaaa tggatttaga aaattatgag gatacattaa attctcctaa aagagaatta gaaataaacg aattaccaaa gaatttcact tggggagacc catggaataa aaataccaga gaatatgagg ttactaatca attattatgt ggttcatgtt atatagcttc acaattatat gcatttaaaa gaagaataga agtggcactt accaaaaaat tggatagaaa atatttgaac aattttgatg atcaattatc catacaaact gttttatcat gttcttttta tgaccaagga tgtaatggtg gattcccata tttagtatct aagctagcta aattacaagg tatcccatta aatgtatatt tcccatatag tgcaactgag gaaacttgtc catataatat aagcaaacat cctaatgata tgaatggttc tgctaaatta agagaaatca atgctatatt taatagtaac aataatatga gtacttataa caacattaat aatgaccacc accaattagg tgtatacgca aatactgcat cttcacaaga acaacatggt atttctgaag aaaacagatg gtacgctaaa gattttaatt atgtaggtgg ttgttatgga tgtaaccaat gtaatggtga gaaaattatg atgaatgaaa tttatagaaa tggtcctatc gtttcttctt ttgaagcttc tccagatttt tatgactatg ctgatggtgt gtattttgtt gaagatttcc cacacgctcg tagatgtacc atcgaaccta aaaacgatgg tgtctataac ataacaggtt gggatcgtgt taaccatgcc attgttttgt taggatgggg agaagaagaa attaatggaa aattatacaa atattggata ggaagaaata gttggggaaa tggttggggt aaagaaggat actttaaaat tttaagagga caaaatttca gtggtattga aagtcagagt ttgtttattg aacctgattt ttcaagaggt gctggtaaaa tacttttaga aaaaatgcaa aaggaattag gaaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat