PlfaA.18756.a

Histidine--tRNA ligase, putative

CENTER ID: PlfaA.18756.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18756.a.A3.GE37790 N term undefined region 601 1132
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18756.a.A3.PW37483 N term undefined region 601 1132
PlfaA.18756.a.A3.PW37578 N term undefined region 601 1132

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1445100
RefSeq: XP_001348602.1
UniProt: Q8IL22

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSISIYKSKV FNSKDIVDIS IHEKNLKVEP SSFKDCFYRL NDKRINIDEL ENKIEYHLLN DEIRNNNNNN YDNSVKKEYI VEKDQNDEKC NNKKTNVFDI KLNNDLNYNM HLTSNNLEEI KCLYFFFLIN ILRFKNNLDL NVIRSICTHI NNISNNDKNN NQYMCIDKIK ENCLYSLKLK DFFVSVLKQC GKNEYNLNDF NRSIHIVIEK SSVIFLNTYK IVSSCKYLTC CFANLLEIFN LNYDILFMNS FNNNINNSNI KSINNIVSNL KWMLHDSKIE KNSNLSKKFS SNILLYIYTQ SKLTDECEYF MNLINQNFKN SIESFTHEYN EEMTSIDTYI NILCNNMNQT FYIYIDNLLN MCKNIMPLYI EKLNEFLLSE ERIITHNVNF KKPPVDGIIL KDMEKQEDEH KGDIKLNLVN IYEKNILDKI ELMFKQISEQ VQKNNLPNSD IICNQKENDN TPKNQYRYLN NSPVEMLKKL NCVFINVTHL ITDIIMHLNI LNDIRAYNKA ISQHMKQKKV SSCVHNVGVG CNEFKNFLYS FLSSENNILL NKSYVNKDKK LVTSHFYDFL EKYFSPYKYE EELNEILLPK NINFNLKTAK GCKDFTGEDM QLRNIFFEYI KKKFLLHGAV EIDTPIFELK ETLMNKYGED SKLIFDLKDQ GGESLSLRYD LTVPLYRFIN TNNVSHLKRF HIGKVYRRDE PSMNRGRFRE FYQCDFDIVG KFDTLRTDFH ILYIFWDILY NLRNVLGNFK CKLNHRKILE YMLLSCNIHK DKVKTVSSSI DKLDKITFQQ FRDELLNDKG IHIEAIDKIE QYISKTLSLS PFLVIEFLRN DLKESTLNEE YKLEIQNCIN HLEQIFDLLK HFNMLNQFSF DLSLARGLDY YTGIIFEFVL LSETNVGSVA AGGRYDFLIR NKRREYLPSV GASIGIERII TIAEEFIRTK NLFCKTKDET TNKDNKNNHQ NHVNVQIDEN VQNKKKTNIS NKEIKPKSSN NKNNSNNSNN NNSENSNKLN MKDNAVDVLV CNIKKDCFKE IVELCTKLWE NDISTEFTYV KDQKIQKQLV YALEKQIPLV LIIGDEIEQG IVKLREITLD KDKSTGEKEI NINDCIQEIK KYFTHNCKWK QNITNILFEK KQ
NT Sequence
atgagtataa gtatttacaa aagtaaagta ttcaatagca aggatattgt ggatattagc atccatgaaa aaaatttgaa agtagagcct tccagcttta aagattgttt ttatagattg aatgataaaa gaataaatat tgacgaatta gaaaataaaa tagaatatca cttattaaat gatgaaatta ggaacaataa taataataat tatgataata gtgtgaagaa agaatatatt gttgaaaaag atcaaaatga tgagaaatgt aataataaaa aaacaaatgt ttttgatata aaattaaata atgatttaaa ttataatatg catttaacat ctaataattt agaagaaata aaatgtttgt attttttctt tttaattaat attttaagat ttaaaaataa tttagatcta aatgttattc gaagtatatg tactcatatt aataatattt caaataatga caagaacaat aatcaatata tgtgtattga taagataaaa gaaaattgtt tgtatagttt aaaattaaaa gatttttttg tttctgtttt aaaacaatgt ggtaagaatg aatataattt gaatgatttt aatagatcta tacatatagt tattgaaaag agtagtgtca tatttttgaa tacttataaa attgtttctt cttgtaaata tttaacttgc tgttttgcca atcttttaga aatttttaat ttaaattatg atattttgtt tatgaattca tttaataata atataaataa tagtaatatc aaatctatta ataacatagt atcgaattta aaatggatgt tacatgattc aaaaattgaa aaaaattcaa acttatcaaa aaagtttagt tcgaacattt tgttatatat ctatacgcaa agtaaattga ccgatgaatg tgaatatttt atgaatctta ttaatcaaaa ttttaaaaat tcaattgaat cctttactca tgaatataac gaagaaatga catccattga tacttatatt aatattttgt gtaataatat gaatcaaaca ttttatatat atatagacaa tttattaaac atgtgtaaaa atattatgcc attatatata gaaaaattaa atgaattttt gttatctgaa gaaagaatta ttactcataa tgtaaatttt aaaaagcccc cggttgacgg aattatttta aaagatatgg aaaaacagga ggatgaacat aaaggtgaca ttaaattaaa tcttgtaaat atatatgaaa agaatatatt agataaaatt gaattaatgt ttaaacaaat aagtgaacaa gtacaaaaga ataatctacc taattctgat ataatatgta atcaaaaaga aaatgataat actcctaaaa atcaatatag atacctgaat aatagtcctg ttgaaatgtt aaaaaaattg aattgtgtat ttataaatgt tactcatctt attacagata taatcatgca tcttaatatt ttgaatgata tacgagcata taacaaggcg atttcgcaac atatgaagca gaaaaaagta tctagttgtg tacataatgt cggtgtaggt tgtaatgaat ttaagaattt cttatactca tttttatcaa gtgaaaataa tatattatta aataaatcct atgtaaataa agataaaaaa cttgttactt cccattttta tgattttctt gaaaaatatt tctcaccata taaatatgaa gaagaattaa atgaaatatt attacctaaa aatattaact ttaatttaaa aacagctaaa ggatgtaaag attttacagg tgaggatatg caattgagga atatattttt tgaatatatt aaaaaaaaat ttttattaca tggagctgtt gaaatagata cgcctatatt tgaattgaaa gaaaccttaa tgaataaata tggtgaagat tcgaaattaa tatttgattt aaaagatcaa ggaggggaaa gtttatcttt aagatatgat ttaactgtac cattgtatcg ttttattaat acaaataatg ttagtcatct taaaagattt catataggaa aggtatatcg aagagatgaa ccaagtatga atagaggacg cttccgagaa ttttaccaat gtgattttga tatagttgga aagtttgata cactacgtac agattttcat atattatata ttttttggga tatcttatat aatttaagaa atgttcttgg taatttcaaa tgtaaattaa atcatagaaa aatattagaa tatatgttat tatcatgtaa tatacataaa gataaagtaa aaactgtatc tagtagtata gacaaattag ataaaataac ttttcaacaa tttagagatg agctattaaa tgataaagga atacatattg aagcaataga taaaattgaa caatatatat ctaagacatt gagtttatca ccttttcttg ttattgaatt tttaagaaat gatttaaaag aatcaaccct aaatgaagaa tataaattag aaatacaaaa ttgtataaac catttagagc aaatatttga tttgttgaaa cattttaata tgcttaatca attttctttt gatttatctt tagcaagagg tttagattat tataccggaa tcattttcga atttgtacta ctttccgaaa cgaatgtagg tagtgtagct gcagggggaa gatatgattt tttaataaga aataagagaa gagaatatct tccatctgtt ggtgcatcga taggtataga gcgtataata acaattgctg aagaatttat aagaacgaaa aacttatttt gtaaaacaaa agatgaaact acaaataaag ataacaaaaa taatcatcaa aatcatgtaa atgttcaaat tgatgaaaat gttcaaaata agaagaaaac aaatatatct aataaggaaa taaaaccaaa aagcagcaac aataagaaca acagtaataa tagtaataat aataatagtg agaatagtaa taaattaaat atgaaagata atgctgttga tgttctcgta tgtaatatta aaaaggattg cttcaaagaa attgttgagt tatgtacaaa attatgggaa aatgatattt caacagaatt tacttatgtc aaagatcaaa aaatacaaaa acaattagta tatgctcttg aaaagcaaat accacttgtt cttattatag gagatgaaat tgaacaaggt attgttaagt taagagaaat cactttagat aaagataaat caactgggga aaaagaaatt aacataaatg attgtattca agaaataaag aaatatttta cacacaactg caaatggaaa caaaatatta ccaatatttt atttgaaaaa aaacaataa
Details for PlfaA.18756.a.A3.GE37790
HARVESTED ON: 2/14/2014
SEQUENCED ON: 3/10/2014
EXPECTED MW: 65kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 57
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGCKDFTGE DMQLRNIFFE YIKKKFLLHG AVEIDTPIFE LKETLMNKYG EDSKLIFDLK DQGGESLSLR YDLTVPLYRF INTNNVSHLK RFHIGKVYRR DEPSMNRGRF REFYQCDFDI VGKFDTLRTD FHILYIFWDI LYNLRNVLGN FKCKLNHRKI LEYMLLSCNI HKDKVKTVSS SIDKLDKITF QQFRDELLND KGIHIEAIDK IEQYISKTLS LSPFLVIEFL RNDLKESTLN EEYKLEIQNC INHLEQIFDL LKHFNMLNQF SFDLSLARGL DYYTGIIFEF VLLSETNVXV
Validated NT Sequence
cnnnngnant ncnctagann attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggga tgtaaagatt ttacaggtga ggatatgcaa ttgaggaata tattttttga atatattaaa aaaaaatttt tattacatgg agctgttgaa atagatacgc ctatatttga attgaaagaa accttaatga ataaatatgg tgaagattcg aaattaatat ttgatttaaa agatcaagga ggggaaagtt tatctttaag atatgattta actgtaccat tgtatcgttt tattaataca aataatgtta gtcatcttaa aagatttcat ataggaaagg tatatcgaag agatgaacca agtatgaata gaggacgctt ccgagaattt taccaatgtg attttgatat agttggaaag tttgatacac tacgtacaga ttttcatata ttatatattt tttgggatat cttatataat ttaagaaatg ttcttggtaa tttcaaatgt aaattaaatc atagaaaaat attagaatat atgttattat catgtaatat acataaagat aaagtaaaaa ctgtatctag tagtatagac aaattagata aaataacttt tcaacaattt agagatgagc tattaaatga taaaggaata catattgaag caatagataa aattgaacaa tatatatcta agacattgag tttatcacct tttcttgtta ttgaattttt aagaaatgat ttaaaagaat caaccctaaa tgaagaatat aaattagaaa tacaaaattg tataaaccat ttagagcaaa tatttgattt gttgaaacat tttaatatgc ttaatcaatt ttcttttgat ttatctttag caagaggttt agattattat accggaatca ttttcgaatt tgtactactt tccgaaacga atgtantagt gtagctgcng gggaagatat gattttttat agaaataana gananatatc ttccatctgt ggtgcatcga taggtatnga gcgtatanna acattgctga nnnttatana nnaaaactta tttgnaacna anatgaanta caataagnnn cnaaaatatc tcnaatcatg taangntcnn natnnnngnt nnnnnnnnnn aancanttnc nnnnnngnnn ancnnnncnn ccccnnataa annnananaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SGCKDFTGED MQLRNIFFEY IKKKFLLHGA VEIDTPIFEL KETLMNKYGE DSKLIFDLKD QGGESLSLRY DLTVPLYRFI NTNNVSHLKR FHIGKVYRRD EPSMNRGRFR EFYQCDFDIV GKFDTLRTDF HILYIFWDIL YNLRNVLGNF KCKLNHRKIL EYMLLSCNIH KDKVKTVSSS IDKLDKITFQ QFRDELLNDK GIHIEAIDKI EQYISKTLSL SPFLVIEFLR NDLKESTLNE EYKLEIQNCI NHLEQIFDLL KHFNMLNQFS FDLSLARGLD YYTGIIFEFV LLSETNVGSV AAGGRYDFLI RNKRREYLPS VGASIGIERI ITIAEEFIRT KNLFCKTKDE TTNKDNKNNH QNHVNVQIDE NVQNKKKTNI SNKEIKPKSS NNKNNSNNSN NNNSENSNKL NMKDNAVDVL VCNIKKDCFK EIVELCTKLW ENDISTEFTY VKDQKIQKQL VYALEKQIPL VLIIGDEIEQ GIVKLREITL DKDKSTGEKE ININDCIQEI KKYFTHNCKW KQNITNILFE KKQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggg atgtaaagat tttacaggtg aggatatgca attgaggaat atattttttg aatatattaa aaaaaaattt ttattacatg gagctgttga aatagatacg cctatatttg aattgaaaga aaccttaatg aataaatatg gtgaagattc gaaattaata tttgatttaa aagatcaagg aggggaaagt ttatctttaa gatatgattt aactgtacca ttgtatcgtt ttattaatac aaataatgtt agtcatctta aaagatttca tataggaaag gtatatcgaa gagatgaacc aagtatgaat agaggacgct tccgagaatt ttaccaatgt gattttgata tagttggaaa gtttgataca ctacgtacag attttcatat attatatatt ttttgggata tcttatataa tttaagaaat gttcttggta atttcaaatg taaattaaat catagaaaaa tattagaata tatgttatta tcatgtaata tacataaaga taaagtaaaa actgtatcta gtagtataga caaattagat aaaataactt ttcaacaatt tagagatgag ctattaaatg ataaaggaat acatattgaa gcaatagata aaattgaaca atatatatct aagacattga gtttatcacc ttttcttgtt attgaatttt taagaaatga tttaaaagaa tcaaccctaa atgaagaata taaattagaa atacaaaatt gtataaacca tttagagcaa atatttgatt tgttgaaaca ttttaatatg cttaatcaat tttcttttga tttatcttta gcaagaggtt tagattatta taccggaatc attttcgaat ttgtactact ttccgaaacg aatgtaggta gtgtagctgc agggggaaga tatgattttt taataagaaa taagagaaga gaatatcttc catctgttgg tgcatcgata ggtatagagc gtataataac aattgctgaa gaatttataa gaacgaaaaa cttattttgt aaaacaaaag atgaaactac aaataaagat aacaaaaata atcatcaaaa tcatgtaaat gttcaaattg atgaaaatgt tcaaaataag aagaaaacaa atatatctaa taaggaaata aaaccaaaaa gcagcaacaa taagaacaac agtaataata gtaataataa taatagtgag aatagtaata aattaaatat gaaagataat gctgttgatg ttctcgtatg taatattaaa aaggattgct tcaaagaaat tgttgagtta tgtacaaaat tatgggaaaa tgatatttca acagaattta cttatgtcaa agatcaaaaa atacaaaaac aattagtata tgctcttgaa aagcaaatac cacttgttct tattatagga gatgaaattg aacaaggtat tgttaagtta agagaaatca ctttagataa agataaatca actggggaaa aagaaattaa cataaatgat tgtattcaag aaataaagaa atattttaca cacaactgca aatggaaaca aaatattacc aatattttat ttgaaaaaaa acaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for PlfaA.18756.a.A3.PW37483
PURIFICATION DATe: 4/22/2014
CONCENTRATION: 30.9mg/ml
OBSERVED MW: 65kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 57
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGCKDFTGE DMQLRNIFFE YIKKKFLLHG AVEIDTPIFE LKETLMNKYG EDSKLIFDLK DQGGESLSLR YDLTVPLYRF INTNNVSHLK RFHIGKVYRR DEPSMNRGRF REFYQCDFDI VGKFDTLRTD FHILYIFWDI LYNLRNVLGN FKCKLNHRKI LEYMLLSCNI HKDKVKTVSS SIDKLDKITF QQFRDELLND KGIHIEAIDK IEQYISKTLS LSPFLVIEFL RNDLKESTLN EEYKLEIQNC INHLEQIFDL LKHFNMLNQF SFDLSLARGL DYYTGIIFEF VLLSETNVXV
Validated NT Sequence
cnnnngnant ncnctagann attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggga tgtaaagatt ttacaggtga ggatatgcaa ttgaggaata tattttttga atatattaaa aaaaaatttt tattacatgg agctgttgaa atagatacgc ctatatttga attgaaagaa accttaatga ataaatatgg tgaagattcg aaattaatat ttgatttaaa agatcaagga ggggaaagtt tatctttaag atatgattta actgtaccat tgtatcgttt tattaataca aataatgtta gtcatcttaa aagatttcat ataggaaagg tatatcgaag agatgaacca agtatgaata gaggacgctt ccgagaattt taccaatgtg attttgatat agttggaaag tttgatacac tacgtacaga ttttcatata ttatatattt tttgggatat cttatataat ttaagaaatg ttcttggtaa tttcaaatgt aaattaaatc atagaaaaat attagaatat atgttattat catgtaatat acataaagat aaagtaaaaa ctgtatctag tagtatagac aaattagata aaataacttt tcaacaattt agagatgagc tattaaatga taaaggaata catattgaag caatagataa aattgaacaa tatatatcta agacattgag tttatcacct tttcttgtta ttgaattttt aagaaatgat ttaaaagaat caaccctaaa tgaagaatat aaattagaaa tacaaaattg tataaaccat ttagagcaaa tatttgattt gttgaaacat tttaatatgc ttaatcaatt ttcttttgat ttatctttag caagaggttt agattattat accggaatca ttttcgaatt tgtactactt tccgaaacga atgtantagt gtagctgcng gggaagatat gattttttat agaaataana gananatatc ttccatctgt ggtgcatcga taggtatnga gcgtatanna acattgctga nnnttatana nnaaaactta tttgnaacna anatgaanta caataagnnn cnaaaatatc tcnaatcatg taangntcnn natnnnngnt nnnnnnnnnn aancanttnc nnnnnngnnn ancnnnncnn ccccnnataa annnananaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SGCKDFTGED MQLRNIFFEY IKKKFLLHGA VEIDTPIFEL KETLMNKYGE DSKLIFDLKD QGGESLSLRY DLTVPLYRFI NTNNVSHLKR FHIGKVYRRD EPSMNRGRFR EFYQCDFDIV GKFDTLRTDF HILYIFWDIL YNLRNVLGNF KCKLNHRKIL EYMLLSCNIH KDKVKTVSSS IDKLDKITFQ QFRDELLNDK GIHIEAIDKI EQYISKTLSL SPFLVIEFLR NDLKESTLNE EYKLEIQNCI NHLEQIFDLL KHFNMLNQFS FDLSLARGLD YYTGIIFEFV LLSETNVGSV AAGGRYDFLI RNKRREYLPS VGASIGIERI ITIAEEFIRT KNLFCKTKDE TTNKDNKNNH QNHVNVQIDE NVQNKKKTNI SNKEIKPKSS NNKNNSNNSN NNNSENSNKL NMKDNAVDVL VCNIKKDCFK EIVELCTKLW ENDISTEFTY VKDQKIQKQL VYALEKQIPL VLIIGDEIEQ GIVKLREITL DKDKSTGEKE ININDCIQEI KKYFTHNCKW KQNITNILFE KKQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGG ATGTAAAGAT TTTACAGGTG AGGATATGCA ATTGAGGAAT ATATTTTTTG AATATATTAA AAAAAAATTT TTATTACATG GAGCTGTTGA AATAGATACG CCTATATTTG AATTGAAAGA AACCTTAATG AATAAATATG GTGAAGATTC GAAATTAATA TTTGATTTAA AAGATCAAGG AGGGGAAAGT TTATCTTTAA GATATGATTT AACTGTACCA TTGTATCGTT TTATTAATAC AAATAATGTT AGTCATCTTA AAAGATTTCA TATAGGAAAG GTATATCGAA GAGATGAACC AAGTATGAAT AGAGGACGCT TCCGAGAATT TTACCAATGT GATTTTGATA TAGTTGGAAA GTTTGATACA CTACGTACAG ATTTTCATAT ATTATATATT TTTTGGGATA TCTTATATAA TTTAAGAAAT GTTCTTGGTA ATTTCAAATG TAAATTAAAT CATAGAAAAA TATTAGAATA TATGTTATTA TCATGTAATA TACATAAAGA TAAAGTAAAA ACTGTATCTA GTAGTATAGA CAAATTAGAT AAAATAACTT TTCAACAATT TAGAGATGAG CTATTAAATG ATAAAGGAAT ACATATTGAA GCAATAGATA AAATTGAACA ATATATATCT AAGACATTGA GTTTATCACC TTTTCTTGTT ATTGAATTTT TAAGAAATGA TTTAAAAGAA TCAACCCTAA ATGAAGAATA TAAATTAGAA ATACAAAATT GTATAAACCA TTTAGAGCAA ATATTTGATT TGTTGAAACA TTTTAATATG CTTAATCAAT TTTCTTTTGA TTTATCTTTA GCAAGAGGTT TAGATTATTA TACCGGAATC ATTTTCGAAT TTGTACTACT TTCCGAAACG AATGTAGGTA GTGTAGCTGC AGGGGGAAGA TATGATTTTT TAATAAGAAA TAAGAGAAGA GAATATCTTC CATCTGTTGG TGCATCGATA GGTATAGAGC GTATAATAAC AATTGCTGAA GAATTTATAA GAACGAAAAA CTTATTTTGT AAAACAAAAG ATGAAACTAC AAATAAAGAT AACAAAAATA ATCATCAAAA TCATGTAAAT GTTCAAATTG ATGAAAATGT TCAAAATAAG AAGAAAACAA ATATATCTAA TAAGGAAATA AAACCAAAAA GCAGCAACAA TAAGAACAAC AGTAATAATA GTAATAATAA TAATAGTGAG AATAGTAATA AATTAAATAT GAAAGATAAT GCTGTTGATG TTCTCGTATG TAATATTAAA AAGGATTGCT TCAAAGAAAT TGTTGAGTTA TGTACAAAAT TATGGGAAAA TGATATTTCA ACAGAATTTA CTTATGTCAA AGATCAAAAA ATACAAAAAC AATTAGTATA TGCTCTTGAA AAGCAAATAC CACTTGTTCT TATTATAGGA GATGAAATTG AACAAGGTAT TGTTAAGTTA AGAGAAATCA CTTTAGATAA AGATAAATCA ACTGGGGAAA AAGAAATTAA CATAAATGAT TGTATTCAAG AAATAAAGAA ATATTTTACA CACAACTGCA AATGGAAACA AAATATTACC AATATTTTAT TTGAAAAAAA ACAATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for PlfaA.18756.a.A3.PW37578
PURIFICATION DATe: 10/22/2014
CONCENTRATION: 24.5mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 57
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGCKDFTGE DMQLRNIFFE YIKKKFLLHG AVEIDTPIFE LKETLMNKYG EDSKLIFDLK DQGGESLSLR YDLTVPLYRF INTNNVSHLK RFHIGKVYRR DEPSMNRGRF REFYQCDFDI VGKFDTLRTD FHILYIFWDI LYNLRNVLGN FKCKLNHRKI LEYMLLSCNI HKDKVKTVSS SIDKLDKITF QQFRDELLND KGIHIEAIDK IEQYISKTLS LSPFLVIEFL RNDLKESTLN EEYKLEIQNC INHLEQIFDL LKHFNMLNQF SFDLSLARGL DYYTGIIFEF VLLSETNVXV
Validated NT Sequence
cnnnngnant ncnctagann attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggga tgtaaagatt ttacaggtga ggatatgcaa ttgaggaata tattttttga atatattaaa aaaaaatttt tattacatgg agctgttgaa atagatacgc ctatatttga attgaaagaa accttaatga ataaatatgg tgaagattcg aaattaatat ttgatttaaa agatcaagga ggggaaagtt tatctttaag atatgattta actgtaccat tgtatcgttt tattaataca aataatgtta gtcatcttaa aagatttcat ataggaaagg tatatcgaag agatgaacca agtatgaata gaggacgctt ccgagaattt taccaatgtg attttgatat agttggaaag tttgatacac tacgtacaga ttttcatata ttatatattt tttgggatat cttatataat ttaagaaatg ttcttggtaa tttcaaatgt aaattaaatc atagaaaaat attagaatat atgttattat catgtaatat acataaagat aaagtaaaaa ctgtatctag tagtatagac aaattagata aaataacttt tcaacaattt agagatgagc tattaaatga taaaggaata catattgaag caatagataa aattgaacaa tatatatcta agacattgag tttatcacct tttcttgtta ttgaattttt aagaaatgat ttaaaagaat caaccctaaa tgaagaatat aaattagaaa tacaaaattg tataaaccat ttagagcaaa tatttgattt gttgaaacat tttaatatgc ttaatcaatt ttcttttgat ttatctttag caagaggttt agattattat accggaatca ttttcgaatt tgtactactt tccgaaacga atgtantagt gtagctgcng gggaagatat gattttttat agaaataana gananatatc ttccatctgt ggtgcatcga taggtatnga gcgtatanna acattgctga nnnttatana nnaaaactta tttgnaacna anatgaanta caataagnnn cnaaaatatc tcnaatcatg taangntcnn natnnnngnt nnnnnnnnnn aancanttnc nnnnnngnnn ancnnnncnn ccccnnataa annnananaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SGCKDFTGED MQLRNIFFEY IKKKFLLHGA VEIDTPIFEL KETLMNKYGE DSKLIFDLKD QGGESLSLRY DLTVPLYRFI NTNNVSHLKR FHIGKVYRRD EPSMNRGRFR EFYQCDFDIV GKFDTLRTDF HILYIFWDIL YNLRNVLGNF KCKLNHRKIL EYMLLSCNIH KDKVKTVSSS IDKLDKITFQ QFRDELLNDK GIHIEAIDKI EQYISKTLSL SPFLVIEFLR NDLKESTLNE EYKLEIQNCI NHLEQIFDLL KHFNMLNQFS FDLSLARGLD YYTGIIFEFV LLSETNVGSV AAGGRYDFLI RNKRREYLPS VGASIGIERI ITIAEEFIRT KNLFCKTKDE TTNKDNKNNH QNHVNVQIDE NVQNKKKTNI SNKEIKPKSS NNKNNSNNSN NNNSENSNKL NMKDNAVDVL VCNIKKDCFK EIVELCTKLW ENDISTEFTY VKDQKIQKQL VYALEKQIPL VLIIGDEIEQ GIVKLREITL DKDKSTGEKE ININDCIQEI KKYFTHNCKW KQNITNILFE KKQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGG ATGTAAAGAT TTTACAGGTG AGGATATGCA ATTGAGGAAT ATATTTTTTG AATATATTAA AAAAAAATTT TTATTACATG GAGCTGTTGA AATAGATACG CCTATATTTG AATTGAAAGA AACCTTAATG AATAAATATG GTGAAGATTC GAAATTAATA TTTGATTTAA AAGATCAAGG AGGGGAAAGT TTATCTTTAA GATATGATTT AACTGTACCA TTGTATCGTT TTATTAATAC AAATAATGTT AGTCATCTTA AAAGATTTCA TATAGGAAAG GTATATCGAA GAGATGAACC AAGTATGAAT AGAGGACGCT TCCGAGAATT TTACCAATGT GATTTTGATA TAGTTGGAAA GTTTGATACA CTACGTACAG ATTTTCATAT ATTATATATT TTTTGGGATA TCTTATATAA TTTAAGAAAT GTTCTTGGTA ATTTCAAATG TAAATTAAAT CATAGAAAAA TATTAGAATA TATGTTATTA TCATGTAATA TACATAAAGA TAAAGTAAAA ACTGTATCTA GTAGTATAGA CAAATTAGAT AAAATAACTT TTCAACAATT TAGAGATGAG CTATTAAATG ATAAAGGAAT ACATATTGAA GCAATAGATA AAATTGAACA ATATATATCT AAGACATTGA GTTTATCACC TTTTCTTGTT ATTGAATTTT TAAGAAATGA TTTAAAAGAA TCAACCCTAA ATGAAGAATA TAAATTAGAA ATACAAAATT GTATAAACCA TTTAGAGCAA ATATTTGATT TGTTGAAACA TTTTAATATG CTTAATCAAT TTTCTTTTGA TTTATCTTTA GCAAGAGGTT TAGATTATTA TACCGGAATC ATTTTCGAAT TTGTACTACT TTCCGAAACG AATGTAGGTA GTGTAGCTGC AGGGGGAAGA TATGATTTTT TAATAAGAAA TAAGAGAAGA GAATATCTTC CATCTGTTGG TGCATCGATA GGTATAGAGC GTATAATAAC AATTGCTGAA GAATTTATAA GAACGAAAAA CTTATTTTGT AAAACAAAAG ATGAAACTAC AAATAAAGAT AACAAAAATA ATCATCAAAA TCATGTAAAT GTTCAAATTG ATGAAAATGT TCAAAATAAG AAGAAAACAA ATATATCTAA TAAGGAAATA AAACCAAAAA GCAGCAACAA TAAGAACAAC AGTAATAATA GTAATAATAA TAATAGTGAG AATAGTAATA AATTAAATAT GAAAGATAAT GCTGTTGATG TTCTCGTATG TAATATTAAA AAGGATTGCT TCAAAGAAAT TGTTGAGTTA TGTACAAAAT TATGGGAAAA TGATATTTCA ACAGAATTTA CTTATGTCAA AGATCAAAAA ATACAAAAAC AATTAGTATA TGCTCTTGAA AAGCAAATAC CACTTGTTCT TATTATAGGA GATGAAATTG AACAAGGTAT TGTTAAGTTA AGAGAAATCA CTTTAGATAA AGATAAATCA ACTGGGGAAA AAGAAATTAA CATAAATGAT TGTATTCAAG AAATAAAGAA ATATTTTACA CACAACTGCA AATGGAAACA AAATATTACC AATATTTTAT TTGAAAAAAA ACAATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA