PlfaA.18708.a

Isoleucine-tRNA ligase, putative (EC 6.1.1.5)

CENTER ID: PlfaA.18708.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18708.a.B3.GE37870 soluble domain 2 965 1272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1332900
RefSeq: XP_001350060.1
UniProt: Q8IDZ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRFVNESFL KVSNKELVHI NIHISRNNLF FNKRIIRRVD NIIRVDIDRL SYYISGYRVN KLLSFVNFNK RIHTLRLEEM VTLTFTGISD NPNIVEEEER ILKYWEDIDA FNLSNELSKN KKPYIFYDGP PFATGLPHYG HLLAGIIKDC VTRYFYQSNF YVERRFGWDC HGLPVEYEIE KENNINKRED ILNMGIDVYN EKCRGIVLKY SKEWVATVQR IGRWIDFKND YKTMDKSFME SVWWVFSELY KNNYIYKSFK VMPYSCKCNT PISNFELNLN YKDTPDPSII ISFILCSDFP KVKNVYNIQD DIQPLVQKYS VLYDCYMKSE KCDTSNEKHA DEDIYSDHIN VNKNNNKNNN SNNNNNNNTN CNDNNNINLN YNNNCYAQHC EVFAWTTTPW TLPSNLALCV NEHFIYLRVY HIESQRMFIF AECRLEWIMK ELKWNVENIK IVNRFLGSEL KELKYKPLFT NFYEKHNFKE RAYKIIADDF VSDDAGTGIV HCAPSYGEDD YRVCCKYGII DPESNILIDP LDWNGYFTNE VEELKDMYIK DADNVIKKKL KENNRLMSNN TIVHSYPFCW RSDTPLIYRA IPAWFVRVSN STNKLVENNE TTYWIPAHVK EKKFHNWIKD AKDWCISRNR YWGTPIPIWC DEKMETVICV ESIKHLEELS GIKNINDLHR HYIDNIEIKN PKGDTYPKLK RIPEVFDCWF ESGSMPYAKV HYPFSTETND FHKIFPADFI AEGLDQTRGW FYTLLVISTL LFNKAPFKNL ICNGLVLASD GKKMSKRLKN YPDPLYILNK YGADSLRLYL INSVAVRAEN LKFQEKGVNE IVKSFILPYY HSFRFFSQEV TRYETTNKMQ FLFNTDYIYK NDNIMDQWIF SSLQSLINSV HTEMKAYKLY NVLPKLLNFI ENLTNWYIRL NRDRMRGMLG KENTHQSLNV LCRTLYLFTI IMAPFTPFIS EYIYQFLKNI KYTNNQNMEH NENNETTNIK EEDLNRNDIH KSVHFIMLPQ VDDKYIIKYE FIELIEHMKD VILLGRNLRE KRKTPNKKPL KSLTILHKNE SFFKHFDRIS NYIKEELNIL NVEYSNDISC LNFSAIPNFK KLGVKLGYNI KNIQNKIKNM NAEDIKLFQQ NKQIIIDNIL LEQDDIIIQM NHNIQNDNTE AISNNYITIL MDFTADQQLE NMASAREICN HIQKIRKNLS LNQNSPVLMH IYIYDQTFKN HMLNENEYIK KCLRRNLNII DLEKDVQNLT DKFFEEKITV NEKEVLVIFT NQ
NT Sequence
atgttaagat ttgtcaatga atcttttttg aaggtttcaa ataaagagct tgtacatata aatatacata taagtagaaa caatttattt tttaataaaa ggataataag aagagttgat aatattataa gagtagatat agatagatta agttattaca tatcaggata tcgtgttaat aaattgttat cgtttgttaa ttttaataaa agaatccata cattaagatt agaagaaatg gtaacattaa catttacagg aataagtgat aatccaaata tagtagagga agaagaaagg atattaaaat attgggaaga tattgatgca tttaatttat caaatgaatt atctaaaaat aaaaagccat atatatttta tgatggacct ccctttgcaa caggattacc acattatggt catttattag ctgggattat aaaagattgt gttactagat atttttatca atccaatttt tatgttgaga gaaggtttgg atgggattgc catggattac ccgttgaata tgaaatagaa aaagagaata atattaataa aagagaagat atattaaata tgggtattga tgtatataat gaaaaatgtc gaggaattgt tttaaaatat tctaaagaat gggtagcaac cgttcaacgt attggtaggt ggattgattt taagaatgat tataaaacta tggataaaag ttttatggaa agtgtttggt gggtttttag tgaattatat aaaaataatt atatttataa atcttttaaa gttatgccct attcatgtaa atgtaataca cccatatcca attttgaatt aaatttaaat tataaagata caccagatcc aagtattata attagtttta tattatgttc tgatttcccg aaggtcaaaa atgtatataa tatacaagat gatatccaac cacttgttca gaaatattcg gttctttatg attgttatat gaaaagcgaa aaatgtgaca cgtccaatga aaaacatgct gatgaggata tttacagtga tcacattaat gttaataaaa ataataataa aaataataat agtaataata ataataataa taatacaaat tgtaatgata ataataatat aaatttaaat tataataaca attgttatgc tcaacattgt gaagtgtttg catggaccac cacaccctgg accttgccat caaaccttgc tctctgtgtt aatgaacatt tcatttattt aagagtgtat catatagaaa gtcaaagaat gtttattttt gctgaatgta gattagaatg gattatgaaa gaattaaaat ggaatgtaga aaatatcaaa attgtgaatc gttttcttgg tagtgaacta aaagaattaa aatataaacc cttatttacg aatttttatg aaaaacataa ttttaaagaa agagcatata aaataattgc tgatgatttt gtaagtgatg atgctggtac aggtatcgta cattgtgctc cgtcttatgg tgaagacgat tatcgagtat gttgtaaata tggtatcata gatccggaaa gtaatatatt aattgatcct ctagattgga atggatattt tactaacgaa gtagaagaat taaaagatat gtatataaaa gatgctgata atgttataaa gaaaaagtta aaagaaaata atcgactaat gagtaataat actattgtcc actcgtatcc attttgttgg agaagtgata caccattaat atatagagct atcccagctt ggtttgtacg tgtaagtaat tcaacaaata aattagttga gaataatgaa acaacttatt ggatcccagc acatgtaaaa gagaaaaaat ttcataattg gattaaggat gcaaaagatt ggtgtattag tagaaataga tattggggta cacctatacc tatatggtgt gatgaaaaaa tggaaactgt tatatgtgtt gaaagtataa aacatttaga agaattatct ggtattaaaa atattaatga tttacataga cattatattg ataatattga aataaaaaat cccaaaggtg atacatatcc caaattaaaa agaataccag aagtttttga ttgttggttt gaaagtggtt ctatgcctta tgctaaagta cattatccat tttctacaga aacaaatgat ttccataaaa tattccctgc agattttata gcagaaggat tagatcaaac tcgaggttgg ttttatacat tactagttat tagtacatta ctttttaata aggctccttt taaaaattta atttgtaatg gtttagttct agcatctgat ggaaaaaaaa tgagtaaacg tttaaaaaat tatccagatc cattatatat attaaataaa tatggtgctg atagtttacg attatattta ataaattcag ttgctgtacg tgctgaaaat ttaaaatttc aagaaaaagg agtaaacgaa attgtcaaaa gttttatatt accatactat catagtttta gattcttttc tcaagaagtt acaagatatg aaactacaaa taagatgcaa ttcttattta atacagatta tatttataaa aatgacaata ttatggatca atggattttt tcctctttac aaagtttaat aaattcagta catacagaaa tgaaggcata taaattatat aatgtacttc ctaagttatt aaattttata gagaatttaa caaattggta tattagattg aatagagatc gtatgagagg tatgttaggt aaagaaaata ctcaccaatc attaaatgtt ttatgtagaa cattatattt gtttacaata attatggccc cttttacacc tttcatctcg gaatatattt accaattttt aaaaaatata aaatatacaa ataaccaaaa tatggaacat aatgaaaata acgaaacaac taatataaaa gaggaagacc taaatcgaaa cgatatacat aaaagtgtac attttattat gttaccacaa gtagatgaca aatatattat aaaatatgaa tttatagaac ttattgaaca catgaaagat gtcattttgt taggaagaaa tttaagagaa aaacgaaaaa caccaaataa gaaaccattg aaatcattaa caatattaca taaaaatgaa tcatttttta aacattttga tcgtatatct aattatataa aagaagaatt aaatattctt aatgtagaat attccaatga tatttcatgt ttaaattttt cagcaatacc gaattttaag aaattaggag taaaattagg atataatatc aaaaatatac aaaataaaat taaaaatatg aatgcagaag atattaaatt atttcaacaa aataaacaaa ttattataga taatatatta ttagaacaag atgatattat aatacaaatg aatcataata tacaaaatga taatacggaa gctattagta ataattatat tactatatta atggatttta cagctgatca acaattagaa aatatggcta gcgcaagaga aatctgtaat catatacaaa aaatcagaaa aaatttatca ttaaatcaaa actcacctgt actcatgcat atatatatat atgatcaaac atttaaaaat catatgctta acgaaaatga atatatcaaa aaatgcttaa gaagaaatct aaatataatt gatcttgaaa aggatgtaca gaatttgacc gacaaattct tcgaggaaaa aattactgtt aatgaaaagg aggtactcgt catttttacc aaccaataa
Details for PlfaA.18708.a.B3.GE37870
HARVESTED ON: 3/28/2014
SEQUENCED ON: 4/2/2014
EXPECTED MW: 38kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ FLKNIKYTNN QNMEHNENNE TTNIKEEDLN RNDIHKSVHF IMLPQVDDKY IIKYEFIELI EHMKDVILLG RNLREKRKTP NKKPLKSLTI LHKNESFFKH FDRISNYIKE ELNILNVEYS NDISCLNFSA IPNFKKLGVK LGYNIKNIQN KIKNMNAEDI KLFQQNKQII IDNILLEQDD IIIQMNHNIQ NDNTEAISNN YITILMDFTA DQQLENMASA REICNHIQKI RKNLSLNQNS PVLMHIYIYD QTFKNHMLNE NEYIKKCLRR NLNIIDLEKD VQNLTDKFFE EKITVNEKEV LVIFTNQ
Validated NT Sequence
cnnnnnnnnn cnctanatat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat ttttaaaaaa tataaaatat acaaataacc aaaatatgga acataatgaa aataacgaaa caactaatat aaaagaggaa gacctaaatc gaaacgatat acataaaagt gtacatttta ttatgttacc acaagtagat gacaaatata ttataaaata tgaatttata gaacttattg aacacatgaa agatgtcatt ttgttaggaa gaaatttaag agaaaaacga aaaacaccaa ataagaaacc attgaaatca ttaacaatat tacataaaaa tgaatcattt tttaaacatt ttgatcgtat atctaattat ataaaagaag aattaaatat tcttaatgta gaatattcca atgatatttc atgtttaaat ttttcagcaa taccgaattt taagaaatta ggagtaaaat taggatataa tatcaaaaat atacaaaata aaattaaaaa tatgaatgca gaagatatta aattatttca acaaaataaa caaattatta tagataatat attattagaa caagatgata ttataataca aatgaatcat aatatacaaa atgataatac ggaagctatt agtaataatt atattactat attaatggat tttacagctg atcaacaatt agaaaatatg gctagcgcaa gagaaatctg taatcatata caaaaaatca gaaaaaattt atcattaaat caaaactcac ctgtactcat gcatatatat atatatgatc aaacatttaa aaatcatatg cttaacgaaa atgaatatat caaaaaatgc ttaagaagaa atctaaatat aattgatctt gaaaaggatg tacagaattt gaccgacaaa ttcttcgagg aaaaaattac tgttaatgaa aaggaggtac tcgtcatttt taccaaccaa taagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgcncnctga gcatactagc atacctnggn tnnnncnnnn nnnnnnnnnn aaaaannnnn nnannaaaaa nnnnnnnnnn ncnngggnnn aacaananca cnnnnntttt gggntgtgtt nnncccnngt cnntctnnnc annnnnnnnn nngnannnna cnncttnnta acannnnatt ttctcnnnna anaa
Expected Protein Sequence
MAHHHHHHQF LKNIKYTNNQ NMEHNENNET TNIKEEDLNR NDIHKSVHFI MLPQVDDKYI IKYEFIELIE HMKDVILLGR NLREKRKTPN KKPLKSLTIL HKNESFFKHF DRISNYIKEE LNILNVEYSN DISCLNFSAI PNFKKLGVKL GYNIKNIQNK IKNMNAEDIK LFQQNKQIII DNILLEQDDI IIQMNHNIQN DNTEAISNNY ITILMDFTAD QQLENMASAR EICNHIQKIR KNLSLNQNSP VLMHIYIYDQ TFKNHMLNEN EYIKKCLRRN LNIIDLEKDV QNLTDKFFEE KITVNEKEVL VIFTNQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaatttt taaaaaatat aaaatataca aataaccaaa atatggaaca taatgaaaat aacgaaacaa ctaatataaa agaggaagac ctaaatcgaa acgatataca taaaagtgta cattttatta tgttaccaca agtagatgac aaatatatta taaaatatga atttatagaa cttattgaac acatgaaaga tgtcattttg ttaggaagaa atttaagaga aaaacgaaaa acaccaaata agaaaccatt gaaatcatta acaatattac ataaaaatga atcatttttt aaacattttg atcgtatatc taattatata aaagaagaat taaatattct taatgtagaa tattccaatg atatttcatg tttaaatttt tcagcaatac cgaattttaa gaaattagga gtaaaattag gatataatat caaaaatata caaaataaaa ttaaaaatat gaatgcagaa gatattaaat tatttcaaca aaataaacaa attattatag ataatatatt attagaacaa gatgatatta taatacaaat gaatcataat atacaaaatg ataatacgga agctattagt aataattata ttactatatt aatggatttt acagctgatc aacaattaga aaatatggct agcgcaagag aaatctgtaa tcatatacaa aaaatcagaa aaaatttatc attaaatcaa aactcacctg tactcatgca tatatatata tatgatcaaa catttaaaaa tcatatgctt aacgaaaatg aatatatcaa aaaatgctta agaagaaatc taaatataat tgatcttgaa aaggatgtac agaatttgac cgacaaattc ttcgaggaaa aaattactgt taatgaaaag gaggtactcg tcatttttac caaccaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at