PlfaA.18681.a

Prolyl-tRNA synthetase (ProRS, Proline--tRNA ligase)

CENTER ID: PlfaA.18681.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PlfaA.18681.a.B1.GE36745 full length 1 746
PlfaA.18681.a.B2.GE36746 cytoplasmic truncation 7 7 746
PlfaA.18681.a.B4.GE36912 cytoplasmic truncation 249 249 746
PlfaA.18681.a.B5.GE36913 cytoplasmic truncation 256 256 746
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PlfaA.18681.a.B5.PW37311 cytoplasmic truncation 256 256 746
PlfaA.18681.a.B4.PW37398 cytoplasmic truncation 249 249 746
PlfaA.18681.a.B4.PW37539 cytoplasmic truncation 249 249 746
PlfaA.18681.a.B4.PW37539 cytoplasmic truncation 249 249 746
PlfaA.18681.a.B4.PW37539 cytoplasmic truncation 249 249 746
PlfaA.18681.a.B4.PW38348 cytoplasmic truncation 249 249 746
Structures
4NCX
DEPOSITED: 10/25/2013
DETERMINATION: XRay
CLONE: PlfaA.18681.a.B4.GE36912
PROTEIN: PlfaA.18681.a.B4.PW37312
4OLF
DEPOSITED: 1/23/2014
DETERMINATION: XRay
CLONE: PlfaA.18681.a.B4.GE36912
PROTEIN: PlfaA.18681.a.B4.PW37398
4Q15
DEPOSITED: 4/2/2014
DETERMINATION: XRay
CLONE: PlfaA.18681.a.B4.GE36912
PROTEIN: PlfaA.18681.a.B4.PW37398
4WI1
DEPOSITED: 9/25/2014
DETERMINATION: XRay
CLONE: PlfaA.18681.a.B4.GE36912
PROTEIN: PlfaA.18681.a.B4.PW37539
Publications by SSGCID
Biochemical and Structural Characterization of Selective Allosteric Inhibitors of the Plasmodium falciparum Drug Target, Prolyl-tRNA-synthetase
Abendroth JA, Barrett LK, Choi R, Dranow DM, Edwards TE, Lorimer DD, Dumais M, Myler PJ, Nakazawa Hewitt S, Stacy R, Van Voorhis WC, Horst B, Forte B, Hallyburton I, Jansen C, Baragana B, Rivas K, Hulverson MA, Gray DW, Read KD, Kirk K, Wernimont A, Walpole C, Gilbert IH
ACS Infect Dis - 2016
volume 3, issue 1, pages 34-44
PMID: 27798837; PMCID: 27798837
PDB: 4NCX, 4Q15, 4WI1
External Resources
RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1213800
RefSeq: XP_001350543.1
UniProt: Q8I5R7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNNNTNGEII IPQEYLEKSE CLFKELKELN INFKEVKHGL AATIKDLLEM NLENSTNILK NLFLKDKKKN YFLICTLNNK TVDLKNLSNI LKTNNLRFVD ENNLNNILNI QPGCLSPLAI KNDKENIVKL YFDEEIKNMQ EVIIHPLHNY SSLYIKTQDV IKFCESFNHA PEYVQIKEDT TSKARVDKKE DVQEEMAKNE ELQNNNNNNK NNSNSNNNNN NNNNHIKDTI LKGKLLSNNE VEDNKSKDSN ILGITSKKIE NFSDWYTQVI VKSELIEYYD ISGCYILRPA AYYIWECVQA FFNKEIKKLN VENSYFPLFV TKNKLEKEKN HIEGFSPEVA WVTKYGDSNL PEEIAIRPTS ETIMYSVFPK WIRSYRDLPL KLNQWNTVVR WEFKQPTPFI RTREFLWQEG HTAHKNEEEA VKLVFDILDL YRRWYEEYLA VPIIKGIKSE GEKFGGANFT STAEAFISEN GRAIQAATSH YLGTNFAKMF KIEFEDENEV KQYVHQTSWG CTTRSIGIMI MTHGDDKGLV LPPNVSKYKV VIVPIFYKTT DENAIHSYCK DIEKILKNAQ INCVYDDRAS YSPGYKFNHW ELRGIPIRIE VGPKDLQNNS CVIVRRDNNE KCNVKKESVL LETQQMLVDI HKNLFLKAKK KLDDSIVQVT SFSEVMNALN KKKMVLAPWC EDIATEEEIK KETQRLSLNQ TNSETTLSGA MKPLCIPLDQ PPMPPNMKCF WSGKPAKRWC LFGRSY
NT Sequence
atgaataata atacaaatgg agaaatcatt atcccccaag aatatttaga aaagtccgaa tgtttgttta aagaattaaa agaattaaat ataaatttta aggaagtaaa acatggctta gctgctacta ttaaagattt attagaaatg aatttagaga attctacaaa tatattaaag aatttgtttt taaaagataa gaagaaaaat tattttctaa tatgtacatt aaataacaaa actgtcgact taaaaaattt atcaaatatt ttaaaaacaa acaacttaag atttgttgac gaaaacaatt taaataatat tcttaatatc caaccaggtt gtttaagtcc tttggctatt aaaaatgata aagaaaatat agtaaaatta tattttgatg aagagataaa aaatatgcaa gaggttatta tacacccttt acataattat agtagtttat atataaaaac acaggatgta ataaaatttt gtgaatcttt taatcacgct cctgaatatg tacaaattaa agaagatact acatcaaaag cacgtgtaga taaaaaagaa gatgtacaag aagaaatggc caaaaatgaa gaattacaga ataataacaa taataataaa aataatagta atagtaacaa taataataat aataataata atcatatcaa ggataccatt ttaaaaggaa agcttttaag taataatgaa gtagaagata acaaatctaa ggatagtaat attttaggta tcacttcaaa aaaaatagaa aatttttcag attggtatac tcaagttatt gtaaaaagtg aattaattga atattatgat atttcgggtt gttatatatt aagacccgct gcatattata tatgggaatg tgttcaagct ttttttaata aagaaataaa aaaattaaat gttgaaaatt cttatttccc attatttgtt acaaaaaata aattagaaaa agaaaaaaat catatagaag gatttagtcc agaagttgct tgggttacaa aatatggaga ttctaattta cctgaagaaa ttgctataag accaacaagt gaaacaatta tgtattcagt gtttccaaaa tggattcgat cgtatcgtga tttacctctt aaattaaatc aatggaatac tgtggtacgt tgggaattta aacaaccaac tccatttatt agaactcgag aatttttatg gcaagaagga catacagctc acaaaaatga agaagaagct gtaaaattag tctttgatat tctagatttg tataggagat ggtatgaaga atatctagct gtacctatta ttaaaggaat aaaaagtgaa ggagaaaaat ttggaggtgc aaattttact tctactgctg aagcttttat aagcgaaaat ggaagagcca tacaagcagc tacatctcat tatcttggaa caaattttgc aaaaatgttt aaaattgaat ttgaagatga aaatgaagtt aaacaatatg ttcatcaaac atcatggggt tgtaccacta gatctattgg tattatgata atgacacatg gtgatgataa aggattagtt ctaccaccaa atgtttctaa atataaagtt gttattgtac ctatcttcta taaaacaaca gatgaaaatg ctatacactc atattgtaaa gatattgaaa aaatattgaa aaatgcacaa attaattgtg tttatgatga tagagcatct tattctcctg gttataaatt caatcattgg gaattaagag gtattcccat aagaatagaa gttggaccta aagatttaca aaataattct tgtgttattg ttagaagaga taataacgaa aaatgtaatg tcaaaaaaga atccgtatta ttagaaacac aacaaatgct tgttgatatt cataaaaacc tcttcttaaa agcaaagaaa aaattagacg attcaattgt tcaagttacg agttttagtg aagtcatgaa tgcattaaat aaaaagaaaa tggtacttgc accatggtgt gaagatattg caacagaaga agaaattaaa aaagaaactc aaagattatc attaaaccaa acaaacagtg aaacgacatt atctggtgct atgaaacctt tatgtatccc gttagatcaa ccacctatgc caccaaatat gaaatgtttc tggtcaggga aaccagctaa aagatggtgt ttatttggta gaagttatta a
Details for PlfaA.18681.a.B1.GE36745
HARVESTED ON: 2/27/2013
SEQUENCED ON: 1/12/2016
EXPECTED MW: 89kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 40
Validated AA Sequence
MAHHHHHHMN NNTNGEIIIP QEYLEKSECL FKELKELNIN FKEVKHGLAA TIKDLLEMNL ENSTNILKNL FLKDKKKNYF LICTLNNKTV DLKNLSNILK TNNLRFVDEN NLNNILNIQP GCLSPLAIKN DKENIVKLYF DEEIKNMQEV IIHPLHNYSS LYIKTQDVIK FCESFNHAPE YVQIKEDTTS KARVDKKEDV QEEMAKNEEL QNNNNNNKNN SNSNNNNNNN NNHIKDTILK GKLLSNNEVE DNKSXDSNIL XITSKKIENF SXWYTQVIVK SELXEYYDIS GCYILRXXAY XIW
Validated NT Sequence
atggctcacc accaccacca ccatatgaat aataatacaa atggagaaat cattatcccc caagaatatt tagaaaagtc cgaatgtttg tttaaagaat taaaagaatt aaatataaat tttaaggaag taaaacatgg cttagctgct actattaaag atttattaga aatgaattta gagaattcta caaatatatt aaagaatttg tttttaaaag ataagaagaa aaattatttt ctaatatgta cattaaataa caaaactgtc gacttaaaaa atttatcaaa tattttaaaa acaaacaact taagatttgt tgacgaaaac aatttaaata atattcttaa tatccaacca ggttgtttaa gtcctttggc tattaaaaat gataaagaaa atatagtaaa attatatttt gatgaagaga taaaaaatat gcaagaggtt attatacacc ctttacataa ttatagtagt ttatatataa aaacacagga tgtaataaaa ttttgtgaat cttttaatca cgctcctgaa tatgtacaaa ttaaagaaga tactacatca aaagcacgtg tagataaaaa agaagatgta caagaagaaa tggccaaaaa tgaagaatta cagaataata acaataataa taaaaataat agtaatagta acaataataa taataataat aataatcata tcaaggatac cattttaaaa ggaaagcttt taagtaataa tgaagtagaa gataacaaat ctnangatag taatatttta nntatcactt caaaaaaaat agaaaatttt tcanattggt atactcaagt tattgtaaaa agtgaattan ntgaatatta tgatatttcg ggttgttata tattaaganc nnctgcatat nanatatggg
Expected Protein Sequence
MAHHHHHHMN NNTNGEIIIP QEYLEKSECL FKELKELNIN FKEVKHGLAA TIKDLLEMNL ENSTNILKNL FLKDKKKNYF LICTLNNKTV DLKNLSNILK TNNLRFVDEN NLNNILNIQP GCLSPLAIKN DKENIVKLYF DEEIKNMQEV IIHPLHNYSS LYIKTQDVIK FCESFNHAPE YVQIKEDTTS KARVDKKEDV QEEMAKNEEL QNNNNNNKNN SNSNNNNNNN NNHIKDTILK GKLLSNNEVE DNKSKDSNIL GITSKKIENF SDWYTQVIVK SELIEYYDIS GCYILRPAAY YIWECVQAFF NKEIKKLNVE NSYFPLFVTK NKLEKEKNHI EGFSPEVAWV TKYGDSNLPE EIAIRPTSET IMYSVFPKWI RSYRDLPLKL NQWNTVVRWE FKQPTPFIRT REFLWQEGHT AHKNEEEAVK LVFDILDLYR RWYEEYLAVP IIKGIKSEGE KFGGANFTST AEAFISENGR AIQAATSHYL GTNFAKMFKI EFEDENEVKQ YVHQTSWGCT TRSIGIMIMT HGDDKGLVLP PNVSKYKVVI VPIFYKTTDE NAIHSYCKDI EKILKNAQIN CVYDDRASYS PGYKFNHWEL RGIPIRIEVG PKDLQNNSCV IVRRDNNEKC NVKKESVLLE TQQMLVDIHK NLFLKAKKKL DDSIVQVTSF SEVMNALNKK KMVLAPWCED IATEEEIKKE TQRLSLNQTN SETTLSGAMK PLCIPLDQPP MPPNMKCFWS GKPAKRWCLF GRSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata ataatacaaa tggagaaatc attatccccc aagaatattt agaaaagtcc gaatgtttgt ttaaagaatt aaaagaatta aatataaatt ttaaggaagt aaaacatggc ttagctgcta ctattaaaga tttattagaa atgaatttag agaattctac aaatatatta aagaatttgt ttttaaaaga taagaagaaa aattattttc taatatgtac attaaataac aaaactgtcg acttaaaaaa tttatcaaat attttaaaaa caaacaactt aagatttgtt gacgaaaaca atttaaataa tattcttaat atccaaccag gttgtttaag tcctttggct attaaaaatg ataaagaaaa tatagtaaaa ttatattttg atgaagagat aaaaaatatg caagaggtta ttatacaccc tttacataat tatagtagtt tatatataaa aacacaggat gtaataaaat tttgtgaatc ttttaatcac gctcctgaat atgtacaaat taaagaagat actacatcaa aagcacgtgt agataaaaaa gaagatgtac aagaagaaat ggccaaaaat gaagaattac agaataataa caataataat aaaaataata gtaatagtaa caataataat aataataata ataatcatat caaggatacc attttaaaag gaaagctttt aagtaataat gaagtagaag ataacaaatc taaggatagt aatattttag gtatcacttc aaaaaaaata gaaaattttt cagattggta tactcaagtt attgtaaaaa gtgaattaat tgaatattat gatatttcgg gttgttatat attaagaccc gctgcatatt atatatggga atgtgttcaa gcttttttta ataaagaaat aaaaaaatta aatgttgaaa attcttattt cccattattt gttacaaaaa ataaattaga aaaagaaaaa aatcatatag aaggatttag tccagaagtt gcttgggtta caaaatatgg agattctaat ttacctgaag aaattgctat aagaccaaca agtgaaacaa ttatgtattc agtgtttcca aaatggattc gatcgtatcg tgatttacct cttaaattaa atcaatggaa tactgtggta cgttgggaat ttaaacaacc aactccattt attagaactc gagaattttt atggcaagaa ggacatacag ctcacaaaaa tgaagaagaa gctgtaaaat tagtctttga tattctagat ttgtatagga gatggtatga agaatatcta gctgtaccta ttattaaagg aataaaaagt gaaggagaaa aatttggagg tgcaaatttt acttctactg ctgaagcttt tataagcgaa aatggaagag ccatacaagc agctacatct cattatcttg gaacaaattt tgcaaaaatg tttaaaattg aatttgaaga tgaaaatgaa gttaaacaat atgttcatca aacatcatgg ggttgtacca ctagatctat tggtattatg ataatgacac atggtgatga taaaggatta gttctaccac caaatgtttc taaatataaa gttgttattg tacctatctt ctataaaaca acagatgaaa atgctataca ctcatattgt aaagatattg aaaaaatatt gaaaaatgca caaattaatt gtgtttatga tgatagagca tcttattctc ctggttataa attcaatcat tgggaattaa gaggtattcc cataagaata gaagttggac ctaaagattt acaaaataat tcttgtgtta ttgttagaag agataataac gaaaaatgta atgtcaaaaa agaatccgta ttattagaaa cacaacaaat gcttgttgat attcataaaa acctcttctt aaaagcaaag aaaaaattag acgattcaat tgttcaagtt acgagtttta gtgaagtcat gaatgcatta aataaaaaga aaatggtact tgcaccatgg tgtgaagata ttgcaacaga agaagaaatt aaaaaagaaa ctcaaagatt atcattaaac caaacaaaca gtgaaacgac attatctggt gctatgaaac ctttatgtat cccgttagat caaccaccta tgccaccaaa tatgaaatgt ttctggtcag ggaaaccagc taaaagatgg tgtttatttg gtagaagtta ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for PlfaA.18681.a.B2.GE36746
HARVESTED ON: 2/27/2013
SEQUENCED ON: 3/28/2013
EXPECTED MW: 88kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: failed alignment
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGE IIIPQEYLEK SECLFKELKE LNINFKEVKH GLAATIKDLL EMNLENSTNI LKNLFLKDKK KNYFLICTLN NKTVDLKNLS NILKTNNLRF VDENNLNNIL NIQPGCLSPL AIKNDKENIV KLYFDEEIKN MQEVIIHPLH NYSSLYIKTQ DVIKFCESFN HAPEYVQIKE DTTSKARVDK KEDVQEEMAK NEELQNNNNN NKNNSNSNNN NNNNNNHIKD TILKGKLLSN NEVEDNKSKD SNILGITSKK IENFSDWYTQ VIVKSELIEY YDISGCYILR PAAYYIWECV QAFFNKEIKK LNVENSYFPL FVTKNKLEKE KNHIEGFSPE VAWVTKYGDS NLPEEIAIRP TSETIMYSVF PKWIRSYRDL PLKLNQWNTV VRWEFKQPTP FIRTREFLWQ EGHTAHKNEE EAVKLVFDIL DLYRRWYEEY LAVPIIKGIK SEGEKFGGAN FTSTAEAFIS ENGRAIQAAT SHYLGTNFAK MFKIEFEDEN EVKQYVHQTS WGCTTRSIGI MIMTHGDDKG LVLPPNVSKY KVVIVPIFYK TTDENAIHSY CKDIEKILKN AQINCVYDDR ASYSPGYKFN HWELRGIPIR IEVGPKDLQN NSCVIVRRDN NEKCNVKKES VLLETQQMLV DIHKNLFLKA KKKLDDSIVQ VTSFSEVMNA LNKKKMVLAP WCEDIATEEE IKKETQRLSL NQTNSETTLS GAMKPLCIPL DQPPMPPNMK CFWSGKPAKR WCLFGRSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggagaaa tcattatccc ccaagaatat ttagaaaagt ccgaatgttt gtttaaagaa ttaaaagaat taaatataaa ttttaaggaa gtaaaacatg gcttagctgc tactattaaa gatttattag aaatgaattt agagaattct acaaatatat taaagaattt gtttttaaaa gataagaaga aaaattattt tctaatatgt acattaaata acaaaactgt cgacttaaaa aatttatcaa atattttaaa aacaaacaac ttaagatttg ttgacgaaaa caatttaaat aatattctta atatccaacc aggttgttta agtcctttgg ctattaaaaa tgataaagaa aatatagtaa aattatattt tgatgaagag ataaaaaata tgcaagaggt tattatacac cctttacata attatagtag tttatatata aaaacacagg atgtaataaa attttgtgaa tcttttaatc acgctcctga atatgtacaa attaaagaag atactacatc aaaagcacgt gtagataaaa aagaagatgt acaagaagaa atggccaaaa atgaagaatt acagaataat aacaataata ataaaaataa tagtaatagt aacaataata ataataataa taataatcat atcaaggata ccattttaaa aggaaagctt ttaagtaata atgaagtaga agataacaaa tctaaggata gtaatatttt aggtatcact tcaaaaaaaa tagaaaattt ttcagattgg tatactcaag ttattgtaaa aagtgaatta attgaatatt atgatatttc gggttgttat atattaagac ccgctgcata ttatatatgg gaatgtgttc aagctttttt taataaagaa ataaaaaaat taaatgttga aaattcttat ttcccattat ttgttacaaa aaataaatta gaaaaagaaa aaaatcatat agaaggattt agtccagaag ttgcttgggt tacaaaatat ggagattcta atttacctga agaaattgct ataagaccaa caagtgaaac aattatgtat tcagtgtttc caaaatggat tcgatcgtat cgtgatttac ctcttaaatt aaatcaatgg aatactgtgg tacgttggga atttaaacaa ccaactccat ttattagaac tcgagaattt ttatggcaag aaggacatac agctcacaaa aatgaagaag aagctgtaaa attagtcttt gatattctag atttgtatag gagatggtat gaagaatatc tagctgtacc tattattaaa ggaataaaaa gtgaaggaga aaaatttgga ggtgcaaatt ttacttctac tgctgaagct tttataagcg aaaatggaag agccatacaa gcagctacat ctcattatct tggaacaaat tttgcaaaaa tgtttaaaat tgaatttgaa gatgaaaatg aagttaaaca atatgttcat caaacatcat ggggttgtac cactagatct attggtatta tgataatgac acatggtgat gataaaggat tagttctacc accaaatgtt tctaaatata aagttgttat tgtacctatc ttctataaaa caacagatga aaatgctata cactcatatt gtaaagatat tgaaaaaata ttgaaaaatg cacaaattaa ttgtgtttat gatgatagag catcttattc tcctggttat aaattcaatc attgggaatt aagaggtatt cccataagaa tagaagttgg acctaaagat ttacaaaata attcttgtgt tattgttaga agagataata acgaaaaatg taatgtcaaa aaagaatccg tattattaga aacacaacaa atgcttgttg atattcataa aaacctcttc ttaaaagcaa agaaaaaatt agacgattca attgttcaag ttacgagttt tagtgaagtc atgaatgcat taaataaaaa gaaaatggta cttgcaccat ggtgtgaaga tattgcaaca gaagaagaaa ttaaaaaaga aactcaaaga ttatcattaa accaaacaaa cagtgaaacg acattatctg gtgctatgaa acctttatgt atcccgttag atcaaccacc tatgccacca aatatgaaat gtttctggtc agggaaacca gctaaaagat ggtgtttatt tggtagaagt tattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PlfaA.18681.a.B4.GE36912
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 59kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMS NILGITSKKI ENFSDWYTQV IVKSELIEYY DISGCYILRP AAYYIWECVQ AFFNKEIKKL NVENSYFPLF VTKNKLEKEK NHIEGFSPEV AWVTKYGDSN LPEEIAIRPT SETIMYSVFP KWIRSYRDLP LKLNQWNTVV RWEFKQPTPF IRTREFLWQE GHTAHKNEEE AVKLVFDILD LYRRWYEEYL AVPIIKGIKS EGEKFGGANF TSTAEAFISE NGRAIQAATS HYLGTNFAKM FKIEFEDENE VKQYVHQTSW GCTTRSIGIM IMTHGDDKGX VLPPNVSKYK VVIVPIFYKT TDENAIXSYX XXXKNXENAX IXXVXXDXAX XXXGXXSXXX XXXXXXXXSX XXXXXXXXXX XXXXKXXXXX XXXXXKPXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgagtaa tattttaggt atcacttcaa aaaaaataga aaatttttca gattggtata ctcaagttat tgtaaaaagt gaattaattg aatattatga tatttcgggt tgttatatat taagacccgc tgcatattat atatgggaat gtgttcaagc tttttttaat aaagaaataa aaaaattaaa tgttgaaaat tcttatttcc cattatttgt tacaaaaaat aaattagaaa aagaaaaaaa tcatatagaa ggatttagtc cagaagttgc ttgggttaca aaatatggag attctaattt acctgaagaa attgctataa gaccaacaag tgaaacaatt atgtattcag tgtttccaaa atggattcga tcgtatcgtg atttacctct taaattaaat caatggaata ctgtggtacg ttgggaattt aaacaaccaa ctccatttat tagaactcga gaatttttat ggcaagaagg acatacagct cacaaaaatg aagaagaagc tgtaaaatta gtctttgata ttctagattt gtataggaga tggtatgaag aatatctagc tgtacctatt attaaaggaa taaaaagtga aggagaaaaa tttggaggtg caaattttac ttctactgct gaagctttta taagcgaaaa tggaagagcc atacaagcag ctacatctca ttatcttgga acaaattttg caaaaatgtt taaaattgaa tttgaagatg aaaatgaagt taaacaatat gttcatcaaa catcatgggg ttgtaccact agatctattg gtattatgat aatgacacat ggtgatgata aaggantagt tctaccacca aatgtttcta aatataaagt tgttattgta cctatcttct ataaaacaac agatgaaaat gctatacnct catattgnaa natnntgaaa aatantgaaa atgcacnaat tnanngtgtt natgangatn gagcannntn tnnnncnggt nnnaantcan cntnggnana nangnnntnc cnnnanaann naagtnnacn aanattnnna aannnnnntn nnnnnnnnnn nnnnnnannn aaangnnang nnnannnnan ncnnnntnnn tnnaaaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnan cannnannnn nnnnnnnnnn nnnnnnnt
Expected Protein Sequence
MAHHHHHHSN ILGITSKKIE NFSDWYTQVI VKSELIEYYD ISGCYILRPA AYYIWECVQA FFNKEIKKLN VENSYFPLFV TKNKLEKEKN HIEGFSPEVA WVTKYGDSNL PEEIAIRPTS ETIMYSVFPK WIRSYRDLPL KLNQWNTVVR WEFKQPTPFI RTREFLWQEG HTAHKNEEEA VKLVFDILDL YRRWYEEYLA VPIIKGIKSE GEKFGGANFT STAEAFISEN GRAIQAATSH YLGTNFAKMF KIEFEDENEV KQYVHQTSWG CTTRSIGIMI MTHGDDKGLV LPPNVSKYKV VIVPIFYKTT DENAIHSYCK DIEKILKNAQ INCVYDDRAS YSPGYKFNHW ELRGIPIRIE VGPKDLQNNS CVIVRRDNNE KCNVKKESVL LETQQMLVDI HKNLFLKAKK KLDDSIVQVT SFSEVMNALN KKKMVLAPWC EDIATEEEIK KETQRLSLNQ TNSETTLSGA MKPLCIPLDQ PPMPPNMKCF WSGKPAKRWC LFGRSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagtaata ttttaggtat cacttcaaaa aaaatagaaa atttttcaga ttggtatact caagttattg taaaaagtga attaattgaa tattatgata tttcgggttg ttatatatta agacccgctg catattatat atgggaatgt gttcaagctt tttttaataa agaaataaaa aaattaaatg ttgaaaattc ttatttccca ttatttgtta caaaaaataa attagaaaaa gaaaaaaatc atatagaagg atttagtcca gaagttgctt gggttacaaa atatggagat tctaatttac ctgaagaaat tgctataaga ccaacaagtg aaacaattat gtattcagtg tttccaaaat ggattcgatc gtatcgtgat ttacctctta aattaaatca atggaatact gtggtacgtt gggaatttaa acaaccaact ccatttatta gaactcgaga atttttatgg caagaaggac atacagctca caaaaatgaa gaagaagctg taaaattagt ctttgatatt ctagatttgt ataggagatg gtatgaagaa tatctagctg tacctattat taaaggaata aaaagtgaag gagaaaaatt tggaggtgca aattttactt ctactgctga agcttttata agcgaaaatg gaagagccat acaagcagct acatctcatt atcttggaac aaattttgca aaaatgttta aaattgaatt tgaagatgaa aatgaagtta aacaatatgt tcatcaaaca tcatggggtt gtaccactag atctattggt attatgataa tgacacatgg tgatgataaa ggattagttc taccaccaaa tgtttctaaa tataaagttg ttattgtacc tatcttctat aaaacaacag atgaaaatgc tatacactca tattgtaaag atattgaaaa aatattgaaa aatgcacaaa ttaattgtgt ttatgatgat agagcatctt attctcctgg ttataaattc aatcattggg aattaagagg tattcccata agaatagaag ttggacctaa agatttacaa aataattctt gtgttattgt tagaagagat aataacgaaa aatgtaatgt caaaaaagaa tccgtattat tagaaacaca acaaatgctt gttgatattc ataaaaacct cttcttaaaa gcaaagaaaa aattagacga ttcaattgtt caagttacga gttttagtga agtcatgaat gcattaaata aaaagaaaat ggtacttgca ccatggtgtg aagatattgc aacagaagaa gaaattaaaa aagaaactca aagattatca ttaaaccaaa caaacagtga aacgacatta tctggtgcta tgaaaccttt atgtatcccg ttagatcaac cacctatgcc accaaatatg aaatgtttct ggtcagggaa accagctaaa agatggtgtt tatttggtag aagttattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for PlfaA.18681.a.B5.GE36913
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 58kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 76
Validated AA Sequence
MYSVFPKWIR SYRDLPLKLN QWNTVVRWEF KQPTPFIRTR EFLWQEGHTA HKNEEEAVKL VFDILDLYRR WYEEYLAVPI IKGIKSEGEK FGGANFTSTA EAFISENGRA IQAATSHYLG TNFAKMFKIE FEDENEVKQY VHQTSWGCTT RSIGIMIMTH GDDKGLVLPP NVSKYKVVIV PIFYKTTDEN AIHSYCKDIE KILKNAQINC VYDDRASYSP GYKFNHWELR GIPIRIEVGP KDLQNNSCVI VRRDNNEKCN VKKESVLLET QQMLVDIHKN LFLKAKKKLD DSIVQVTSFS EVMNALNKKK MVLAPWCEDI ATEEEIKKET QRLSLNQTNS ETTLSGAMKP LCIPLDQPPM PPNMKCFWSG KPAKRWCLFG RSY
Validated NT Sequence
atcttactca cttaataact tctaccaaat aaacaccatc ttttagctgg tttccctgac cagaaacatt tcatatttgg tggcataggt ggttgatcta acgggataca taaaggtttc atagcaccag ataatgtcgt ttcactgttt gtttggttta atgataatct ttgagtttct tttttaattt cttcttctgt tgcaatatct tcacaccatg gtgcaagtac cattttcttt ttatttaatg cattcatgac ttcactaaaa ctcgtaactt gaacaattga atcgtctaat tttttctttg cttttaagaa gaggttttta tgaatatcaa caagcatttg ttgtgtttct aataatacgg attctttttt gacattacat ttttcgttat tatctcttct aacaataaca caagaattat tttgtaaatc tttaggtcca acttctattc ttatgggaat acctcttaat tcccaatgat tgaatttata accaggagaa taagatgctc tatcatcata aacacaatta atttgtgcat ttttcaatat tttttcaata tctttacaat atgagtgtat agcattttca tctgttgttt tatagaagat aggtacaata acaactttat atttagaaac atttggtggt agaactaatc ctttatcatc accatgtgtc attatcataa taccaataga tctagtggta caaccccatg atgtttgatg aacatattgt ttaacttcat tttcatcttc aaattcaatt ttaaacattt ttgcaaaatt tgttccaaga taatgagatg tagctgcttg tatggctctt ccattttcgc ttataaaagc ttcagcagta gaagtaaaat ttgcacctcc aaatttttct ccttcacttt ttattccttt aataataggt acagctagat attcttcata ccatctccta tacaaatcta gaatatcaaa gactaatttt acagcttctt cttcattttt gtgagctgta tgtccttctt gccataaaaa ttctcgagtt ctaataaatg gagttggttg tttaaattcc caacgtacca cagtattcca ttgatttaat ttaagaggta aatcacgata cgatcgaatc cattttggaa acactgaata cataattgtt tcacttgttg gtcttatagc aatttcttca ggtaaattag aatctccata ttttgtaacc caagcaactt ctggactaaa tccttctata tgattttttt ctttttctaa tttatttttt gtaacaaata atgggaaata agaattttca acatttaatt tttttatttc tttattaaaa aaagcttgaa cacattccca tatataatat gcagcgggtc ttaatatata acaacccgaa atatcataat attcaattaa ttcacttttt acaataactt gagtatacca atctgaaaaa ttttctattt tttt
Expected Protein Sequence
MAHHHHHHSK KIENFSDWYT QVIVKSELIE YYDISGCYIL RPAAYYIWEC VQAFFNKEIK KLNVENSYFP LFVTKNKLEK EKNHIEGFSP EVAWVTKYGD SNLPEEIAIR PTSETIMYSV FPKWIRSYRD LPLKLNQWNT VVRWEFKQPT PFIRTREFLW QEGHTAHKNE EEAVKLVFDI LDLYRRWYEE YLAVPIIKGI KSEGEKFGGA NFTSTAEAFI SENGRAIQAA TSHYLGTNFA KMFKIEFEDE NEVKQYVHQT SWGCTTRSIG IMIMTHGDDK GLVLPPNVSK YKVVIVPIFY KTTDENAIHS YCKDIEKILK NAQINCVYDD RASYSPGYKF NHWELRGIPI RIEVGPKDLQ NNSCVIVRRD NNEKCNVKKE SVLLETQQML VDIHKNLFLK AKKKLDDSIV QVTSFSEVMN ALNKKKMVLA PWCEDIATEE EIKKETQRLS LNQTNSETTL SGAMKPLCIP LDQPPMPPNM KCFWSGKPAK RWCLFGRSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcaaaaa aaatagaaaa tttttcagat tggtatactc aagttattgt aaaaagtgaa ttaattgaat attatgatat ttcgggttgt tatatattaa gacccgctgc atattatata tgggaatgtg ttcaagcttt ttttaataaa gaaataaaaa aattaaatgt tgaaaattct tatttcccat tatttgttac aaaaaataaa ttagaaaaag aaaaaaatca tatagaagga tttagtccag aagttgcttg ggttacaaaa tatggagatt ctaatttacc tgaagaaatt gctataagac caacaagtga aacaattatg tattcagtgt ttccaaaatg gattcgatcg tatcgtgatt tacctcttaa attaaatcaa tggaatactg tggtacgttg ggaatttaaa caaccaactc catttattag aactcgagaa tttttatggc aagaaggaca tacagctcac aaaaatgaag aagaagctgt aaaattagtc tttgatattc tagatttgta taggagatgg tatgaagaat atctagctgt acctattatt aaaggaataa aaagtgaagg agaaaaattt ggaggtgcaa attttacttc tactgctgaa gcttttataa gcgaaaatgg aagagccata caagcagcta catctcatta tcttggaaca aattttgcaa aaatgtttaa aattgaattt gaagatgaaa atgaagttaa acaatatgtt catcaaacat catggggttg taccactaga tctattggta ttatgataat gacacatggt gatgataaag gattagttct accaccaaat gtttctaaat ataaagttgt tattgtacct atcttctata aaacaacaga tgaaaatgct atacactcat attgtaaaga tattgaaaaa atattgaaaa atgcacaaat taattgtgtt tatgatgata gagcatctta ttctcctggt tataaattca atcattggga attaagaggt attcccataa gaatagaagt tggacctaaa gatttacaaa ataattcttg tgttattgtt agaagagata ataacgaaaa atgtaatgtc aaaaaagaat ccgtattatt agaaacacaa caaatgcttg ttgatattca taaaaacctc ttcttaaaag caaagaaaaa attagacgat tcaattgttc aagttacgag ttttagtgaa gtcatgaatg cattaaataa aaagaaaatg gtacttgcac catggtgtga agatattgca acagaagaag aaattaaaaa agaaactcaa agattatcat taaaccaaac aaacagtgaa acgacattat ctggtgctat gaaaccttta tgtatcccgt tagatcaacc acctatgcca ccaaatatga aatgtttctg gtcagggaaa ccagctaaaa gatggtgttt atttggtaga agttattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PlfaA.18681.a.B5.PW37311
PURIFICATION DATe: 6/17/2013
CONCENTRATION: 20.4mg/ml
OBSERVED MW: 60kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 76
Protocol Notes
notes unavailable
Validated AA Sequence
MYSVFPKWIR SYRDLPLKLN QWNTVVRWEF KQPTPFIRTR EFLWQEGHTA HKNEEEAVKL VFDILDLYRR WYEEYLAVPI IKGIKSEGEK FGGANFTSTA EAFISENGRA IQAATSHYLG TNFAKMFKIE FEDENEVKQY VHQTSWGCTT RSIGIMIMTH GDDKGLVLPP NVSKYKVVIV PIFYKTTDEN AIHSYCKDIE KILKNAQINC VYDDRASYSP GYKFNHWELR GIPIRIEVGP KDLQNNSCVI VRRDNNEKCN VKKESVLLET QQMLVDIHKN LFLKAKKKLD DSIVQVTSFS EVMNALNKKK MVLAPWCEDI ATEEEIKKET QRLSLNQTNS ETTLSGAMKP LCIPLDQPPM PPNMKCFWSG KPAKRWCLFG RSY
Validated NT Sequence
atcttactca cttaataact tctaccaaat aaacaccatc ttttagctgg tttccctgac cagaaacatt tcatatttgg tggcataggt ggttgatcta acgggataca taaaggtttc atagcaccag ataatgtcgt ttcactgttt gtttggttta atgataatct ttgagtttct tttttaattt cttcttctgt tgcaatatct tcacaccatg gtgcaagtac cattttcttt ttatttaatg cattcatgac ttcactaaaa ctcgtaactt gaacaattga atcgtctaat tttttctttg cttttaagaa gaggttttta tgaatatcaa caagcatttg ttgtgtttct aataatacgg attctttttt gacattacat ttttcgttat tatctcttct aacaataaca caagaattat tttgtaaatc tttaggtcca acttctattc ttatgggaat acctcttaat tcccaatgat tgaatttata accaggagaa taagatgctc tatcatcata aacacaatta atttgtgcat ttttcaatat tttttcaata tctttacaat atgagtgtat agcattttca tctgttgttt tatagaagat aggtacaata acaactttat atttagaaac atttggtggt agaactaatc ctttatcatc accatgtgtc attatcataa taccaataga tctagtggta caaccccatg atgtttgatg aacatattgt ttaacttcat tttcatcttc aaattcaatt ttaaacattt ttgcaaaatt tgttccaaga taatgagatg tagctgcttg tatggctctt ccattttcgc ttataaaagc ttcagcagta gaagtaaaat ttgcacctcc aaatttttct ccttcacttt ttattccttt aataataggt acagctagat attcttcata ccatctccta tacaaatcta gaatatcaaa gactaatttt acagcttctt cttcattttt gtgagctgta tgtccttctt gccataaaaa ttctcgagtt ctaataaatg gagttggttg tttaaattcc caacgtacca cagtattcca ttgatttaat ttaagaggta aatcacgata cgatcgaatc cattttggaa acactgaata cataattgtt tcacttgttg gtcttatagc aatttcttca ggtaaattag aatctccata ttttgtaacc caagcaactt ctggactaaa tccttctata tgattttttt ctttttctaa tttatttttt gtaacaaata atgggaaata agaattttca acatttaatt tttttatttc tttattaaaa aaagcttgaa cacattccca tatataatat gcagcgggtc ttaatatata acaacccgaa atatcataat attcaattaa ttcacttttt acaataactt gagtatacca atctgaaaaa ttttctattt tttt
Expressed Protein Sequence
MAHHHHHHSK KIENFSDWYT QVIVKSELIE YYDISGCYIL RPAAYYIWEC VQAFFNKEIK KLNVENSYFP LFVTKNKLEK EKNHIEGFSP EVAWVTKYGD SNLPEEIAIR PTSETIMYSV FPKWIRSYRD LPLKLNQWNT VVRWEFKQPT PFIRTREFLW QEGHTAHKNE EEAVKLVFDI LDLYRRWYEE YLAVPIIKGI KSEGEKFGGA NFTSTAEAFI SENGRAIQAA TSHYLGTNFA KMFKIEFEDE NEVKQYVHQT SWGCTTRSIG IMIMTHGDDK GLVLPPNVSK YKVVIVPIFY KTTDENAIHS YCKDIEKILK NAQINCVYDD RASYSPGYKF NHWELRGIPI RIEVGPKDLQ NNSCVIVRRD NNEKCNVKKE SVLLETQQML VDIHKNLFLK AKKKLDDSIV QVTSFSEVMN ALNKKKMVLA PWCEDIATEE EIKKETQRLS LNQTNSETTL SGAMKPLCIP LDQPPMPPNM KCFWSGKPAK RWCLFGRSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCAAAAA AAATAGAAAA TTTTTCAGAT TGGTATACTC AAGTTATTGT AAAAAGTGAA TTAATTGAAT ATTATGATAT TTCGGGTTGT TATATATTAA GACCCGCTGC ATATTATATA TGGGAATGTG TTCAAGCTTT TTTTAATAAA GAAATAAAAA AATTAAATGT TGAAAATTCT TATTTCCCAT TATTTGTTAC AAAAAATAAA TTAGAAAAAG AAAAAAATCA TATAGAAGGA TTTAGTCCAG AAGTTGCTTG GGTTACAAAA TATGGAGATT CTAATTTACC TGAAGAAATT GCTATAAGAC CAACAAGTGA AACAATTATG TATTCAGTGT TTCCAAAATG GATTCGATCG TATCGTGATT TACCTCTTAA ATTAAATCAA TGGAATACTG TGGTACGTTG GGAATTTAAA CAACCAACTC CATTTATTAG AACTCGAGAA TTTTTATGGC AAGAAGGACA TACAGCTCAC AAAAATGAAG AAGAAGCTGT AAAATTAGTC TTTGATATTC TAGATTTGTA TAGGAGATGG TATGAAGAAT ATCTAGCTGT ACCTATTATT AAAGGAATAA AAAGTGAAGG AGAAAAATTT GGAGGTGCAA ATTTTACTTC TACTGCTGAA GCTTTTATAA GCGAAAATGG AAGAGCCATA CAAGCAGCTA CATCTCATTA TCTTGGAACA AATTTTGCAA AAATGTTTAA AATTGAATTT GAAGATGAAA ATGAAGTTAA ACAATATGTT CATCAAACAT CATGGGGTTG TACCACTAGA TCTATTGGTA TTATGATAAT GACACATGGT GATGATAAAG GATTAGTTCT ACCACCAAAT GTTTCTAAAT ATAAAGTTGT TATTGTACCT ATCTTCTATA AAACAACAGA TGAAAATGCT ATACACTCAT ATTGTAAAGA TATTGAAAAA ATATTGAAAA ATGCACAAAT TAATTGTGTT TATGATGATA GAGCATCTTA TTCTCCTGGT TATAAATTCA ATCATTGGGA ATTAAGAGGT ATTCCCATAA GAATAGAAGT TGGACCTAAA GATTTACAAA ATAATTCTTG TGTTATTGTT AGAAGAGATA ATAACGAAAA ATGTAATGTC AAAAAAGAAT CCGTATTATT AGAAACACAA CAAATGCTTG TTGATATTCA TAAAAACCTC TTCTTAAAAG CAAAGAAAAA ATTAGACGAT TCAATTGTTC AAGTTACGAG TTTTAGTGAA GTCATGAATG CATTAAATAA AAAGAAAATG GTACTTGCAC CATGGTGTGA AGATATTGCA ACAGAAGAAG AAATTAAAAA AGAAACTCAA AGATTATCAT TAAACCAAAC AAACAGTGAA ACGACATTAT CTGGTGCTAT GAAACCTTTA TGTATCCCGT TAGATCAACC ACCTATGCCA CCAAATATGA AATGTTTCTG GTCAGGGAAA CCAGCTAAAA GATGGTGTTT ATTTGGTAGA AGTTATTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for PlfaA.18681.a.B4.PW37398
PURIFICATION DATe: 11/18/2013
CONCENTRATION: 24.3mg/ml
OBSERVED MW: 59kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 110µl
PERCENT IDENTITY: 80
PERCENT COVERAGE: 80
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS NILGITSKKI ENFSDWYTQV IVKSELIEYY DISGCYILRP AAYYIWECVQ AFFNKEIKKL NVENSYFPLF VTKNKLEKEK NHIEGFSPEV AWVTKYGDSN LPEEIAIRPT SETIMYSVFP KWIRSYRDLP LKLNQWNTVV RWEFKQPTPF IRTREFLWQE GHTAHKNEEE AVKLVFDILD LYRRWYEEYL AVPIIKGIKS EGEKFGGANF TSTAEAFISE NGRAIQAATS HYLGTNFAKM FKIEFEDENE VKQYVHQTSW GCTTRSIGIM IMTHGDDKGX VLPPNVSKYK VVIVPIFYKT TDENAIXSYX XXXKNXENAX IXXVXXDXAX XXXGXXSXXX XXXXXXXXSX XXXXXXXXXX XXXXKXXXXX XXXXXKPXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgagtaa tattttaggt atcacttcaa aaaaaataga aaatttttca gattggtata ctcaagttat tgtaaaaagt gaattaattg aatattatga tatttcgggt tgttatatat taagacccgc tgcatattat atatgggaat gtgttcaagc tttttttaat aaagaaataa aaaaattaaa tgttgaaaat tcttatttcc cattatttgt tacaaaaaat aaattagaaa aagaaaaaaa tcatatagaa ggatttagtc cagaagttgc ttgggttaca aaatatggag attctaattt acctgaagaa attgctataa gaccaacaag tgaaacaatt atgtattcag tgtttccaaa atggattcga tcgtatcgtg atttacctct taaattaaat caatggaata ctgtggtacg ttgggaattt aaacaaccaa ctccatttat tagaactcga gaatttttat ggcaagaagg acatacagct cacaaaaatg aagaagaagc tgtaaaatta gtctttgata ttctagattt gtataggaga tggtatgaag aatatctagc tgtacctatt attaaaggaa taaaaagtga aggagaaaaa tttggaggtg caaattttac ttctactgct gaagctttta taagcgaaaa tggaagagcc atacaagcag ctacatctca ttatcttgga acaaattttg caaaaatgtt taaaattgaa tttgaagatg aaaatgaagt taaacaatat gttcatcaaa catcatgggg ttgtaccact agatctattg gtattatgat aatgacacat ggtgatgata aaggantagt tctaccacca aatgtttcta aatataaagt tgttattgta cctatcttct ataaaacaac agatgaaaat gctatacnct catattgnaa natnntgaaa aatantgaaa atgcacnaat tnanngtgtt natgangatn gagcannntn tnnnncnggt nnnaantcan cntnggnana nangnnntnc cnnnanaann naagtnnacn aanattnnna aannnnnntn nnnnnnnnnn nnnnnnannn aaangnnang nnnannnnan ncnnnntnnn tnnaaaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnan cannnannnn nnnnnnnnnn nnnnnnnt
Expressed Protein Sequence
MAHHHHHHSN ILGITSKKIE NFSDWYTQVI VKSELIEYYD ISGCYILRPA AYYIWECVQA FFNKEIKKLN VENSYFPLFV TKNKLEKEKN HIEGFSPEVA WVTKYGDSNL PEEIAIRPTS ETIMYSVFPK WIRSYRDLPL KLNQWNTVVR WEFKQPTPFI RTREFLWQEG HTAHKNEEEA VKLVFDILDL YRRWYEEYLA VPIIKGIKSE GEKFGGANFT STAEAFISEN GRAIQAATSH YLGTNFAKMF KIEFEDENEV KQYVHQTSWG CTTRSIGIMI MTHGDDKGLV LPPNVSKYKV VIVPIFYKTT DENAIHSYCK DIEKILKNAQ INCVYDDRAS YSPGYKFNHW ELRGIPIRIE VGPKDLQNNS CVIVRRDNNE KCNVKKESVL LETQQMLVDI HKNLFLKAKK KLDDSIVQVT SFSEVMNALN KKKMVLAPWC EDIATEEEIK KETQRLSLNQ TNSETTLSGA MKPLCIPLDQ PPMPPNMKCF WSGKPAKRWC LFGRSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAGTAATA TTTTAGGTAT CACTTCAAAA AAAATAGAAA ATTTTTCAGA TTGGTATACT CAAGTTATTG TAAAAAGTGA ATTAATTGAA TATTATGATA TTTCGGGTTG TTATATATTA AGACCCGCTG CATATTATAT ATGGGAATGT GTTCAAGCTT TTTTTAATAA AGAAATAAAA AAATTAAATG TTGAAAATTC TTATTTCCCA TTATTTGTTA CAAAAAATAA ATTAGAAAAA GAAAAAAATC ATATAGAAGG ATTTAGTCCA GAAGTTGCTT GGGTTACAAA ATATGGAGAT TCTAATTTAC CTGAAGAAAT TGCTATAAGA CCAACAAGTG AAACAATTAT GTATTCAGTG TTTCCAAAAT GGATTCGATC GTATCGTGAT TTACCTCTTA AATTAAATCA ATGGAATACT GTGGTACGTT GGGAATTTAA ACAACCAACT CCATTTATTA GAACTCGAGA ATTTTTATGG CAAGAAGGAC ATACAGCTCA CAAAAATGAA GAAGAAGCTG TAAAATTAGT CTTTGATATT CTAGATTTGT ATAGGAGATG GTATGAAGAA TATCTAGCTG TACCTATTAT TAAAGGAATA AAAAGTGAAG GAGAAAAATT TGGAGGTGCA AATTTTACTT CTACTGCTGA AGCTTTTATA AGCGAAAATG GAAGAGCCAT ACAAGCAGCT ACATCTCATT ATCTTGGAAC AAATTTTGCA AAAATGTTTA AAATTGAATT TGAAGATGAA AATGAAGTTA AACAATATGT TCATCAAACA TCATGGGGTT GTACCACTAG ATCTATTGGT ATTATGATAA TGACACATGG TGATGATAAA GGATTAGTTC TACCACCAAA TGTTTCTAAA TATAAAGTTG TTATTGTACC TATCTTCTAT AAAACAACAG ATGAAAATGC TATACACTCA TATTGTAAAG ATATTGAAAA AATATTGAAA AATGCACAAA TTAATTGTGT TTATGATGAT AGAGCATCTT ATTCTCCTGG TTATAAATTC AATCATTGGG AATTAAGAGG TATTCCCATA AGAATAGAAG TTGGACCTAA AGATTTACAA AATAATTCTT GTGTTATTGT TAGAAGAGAT AATAACGAAA AATGTAATGT CAAAAAAGAA TCCGTATTAT TAGAAACACA ACAAATGCTT GTTGATATTC ATAAAAACCT CTTCTTAAAA GCAAAGAAAA AATTAGACGA TTCAATTGTT CAAGTTACGA GTTTTAGTGA AGTCATGAAT GCATTAAATA AAAAGAAAAT GGTACTTGCA CCATGGTGTG AAGATATTGC AACAGAAGAA GAAATTAAAA AAGAAACTCA AAGATTATCA TTAAACCAAA CAAACAGTGA AACGACATTA TCTGGTGCTA TGAAACCTTT ATGTATCCCG TTAGATCAAC CACCTATGCC ACCAAATATG AAATGTTTCT GGTCAGGGAA ACCAGCTAAA AGATGGTGTT TATTTGGTAG AAGTTATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for PlfaA.18681.a.B4.PW37539
PURIFICATION DATe: 8/19/2014
CONCENTRATION: 21.9mg/ml
OBSERVED MW: 60kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 110µl
PERCENT IDENTITY: 80
PERCENT COVERAGE: 80
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS NILGITSKKI ENFSDWYTQV IVKSELIEYY DISGCYILRP AAYYIWECVQ AFFNKEIKKL NVENSYFPLF VTKNKLEKEK NHIEGFSPEV AWVTKYGDSN LPEEIAIRPT SETIMYSVFP KWIRSYRDLP LKLNQWNTVV RWEFKQPTPF IRTREFLWQE GHTAHKNEEE AVKLVFDILD LYRRWYEEYL AVPIIKGIKS EGEKFGGANF TSTAEAFISE NGRAIQAATS HYLGTNFAKM FKIEFEDENE VKQYVHQTSW GCTTRSIGIM IMTHGDDKGX VLPPNVSKYK VVIVPIFYKT TDENAIXSYX XXXKNXENAX IXXVXXDXAX XXXGXXSXXX XXXXXXXXSX XXXXXXXXXX XXXXKXXXXX XXXXXKPXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgagtaa tattttaggt atcacttcaa aaaaaataga aaatttttca gattggtata ctcaagttat tgtaaaaagt gaattaattg aatattatga tatttcgggt tgttatatat taagacccgc tgcatattat atatgggaat gtgttcaagc tttttttaat aaagaaataa aaaaattaaa tgttgaaaat tcttatttcc cattatttgt tacaaaaaat aaattagaaa aagaaaaaaa tcatatagaa ggatttagtc cagaagttgc ttgggttaca aaatatggag attctaattt acctgaagaa attgctataa gaccaacaag tgaaacaatt atgtattcag tgtttccaaa atggattcga tcgtatcgtg atttacctct taaattaaat caatggaata ctgtggtacg ttgggaattt aaacaaccaa ctccatttat tagaactcga gaatttttat ggcaagaagg acatacagct cacaaaaatg aagaagaagc tgtaaaatta gtctttgata ttctagattt gtataggaga tggtatgaag aatatctagc tgtacctatt attaaaggaa taaaaagtga aggagaaaaa tttggaggtg caaattttac ttctactgct gaagctttta taagcgaaaa tggaagagcc atacaagcag ctacatctca ttatcttgga acaaattttg caaaaatgtt taaaattgaa tttgaagatg aaaatgaagt taaacaatat gttcatcaaa catcatgggg ttgtaccact agatctattg gtattatgat aatgacacat ggtgatgata aaggantagt tctaccacca aatgtttcta aatataaagt tgttattgta cctatcttct ataaaacaac agatgaaaat gctatacnct catattgnaa natnntgaaa aatantgaaa atgcacnaat tnanngtgtt natgangatn gagcannntn tnnnncnggt nnnaantcan cntnggnana nangnnntnc cnnnanaann naagtnnacn aanattnnna aannnnnntn nnnnnnnnnn nnnnnnannn aaangnnang nnnannnnan ncnnnntnnn tnnaaaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnan cannnannnn nnnnnnnnnn nnnnnnnt
Expressed Protein Sequence
MAHHHHHHSN ILGITSKKIE NFSDWYTQVI VKSELIEYYD ISGCYILRPA AYYIWECVQA FFNKEIKKLN VENSYFPLFV TKNKLEKEKN HIEGFSPEVA WVTKYGDSNL PEEIAIRPTS ETIMYSVFPK WIRSYRDLPL KLNQWNTVVR WEFKQPTPFI RTREFLWQEG HTAHKNEEEA VKLVFDILDL YRRWYEEYLA VPIIKGIKSE GEKFGGANFT STAEAFISEN GRAIQAATSH YLGTNFAKMF KIEFEDENEV KQYVHQTSWG CTTRSIGIMI MTHGDDKGLV LPPNVSKYKV VIVPIFYKTT DENAIHSYCK DIEKILKNAQ INCVYDDRAS YSPGYKFNHW ELRGIPIRIE VGPKDLQNNS CVIVRRDNNE KCNVKKESVL LETQQMLVDI HKNLFLKAKK KLDDSIVQVT SFSEVMNALN KKKMVLAPWC EDIATEEEIK KETQRLSLNQ TNSETTLSGA MKPLCIPLDQ PPMPPNMKCF WSGKPAKRWC LFGRSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAGTAATA TTTTAGGTAT CACTTCAAAA AAAATAGAAA ATTTTTCAGA TTGGTATACT CAAGTTATTG TAAAAAGTGA ATTAATTGAA TATTATGATA TTTCGGGTTG TTATATATTA AGACCCGCTG CATATTATAT ATGGGAATGT GTTCAAGCTT TTTTTAATAA AGAAATAAAA AAATTAAATG TTGAAAATTC TTATTTCCCA TTATTTGTTA CAAAAAATAA ATTAGAAAAA GAAAAAAATC ATATAGAAGG ATTTAGTCCA GAAGTTGCTT GGGTTACAAA ATATGGAGAT TCTAATTTAC CTGAAGAAAT TGCTATAAGA CCAACAAGTG AAACAATTAT GTATTCAGTG TTTCCAAAAT GGATTCGATC GTATCGTGAT TTACCTCTTA AATTAAATCA ATGGAATACT GTGGTACGTT GGGAATTTAA ACAACCAACT CCATTTATTA GAACTCGAGA ATTTTTATGG CAAGAAGGAC ATACAGCTCA CAAAAATGAA GAAGAAGCTG TAAAATTAGT CTTTGATATT CTAGATTTGT ATAGGAGATG GTATGAAGAA TATCTAGCTG TACCTATTAT TAAAGGAATA AAAAGTGAAG GAGAAAAATT TGGAGGTGCA AATTTTACTT CTACTGCTGA AGCTTTTATA AGCGAAAATG GAAGAGCCAT ACAAGCAGCT ACATCTCATT ATCTTGGAAC AAATTTTGCA AAAATGTTTA AAATTGAATT TGAAGATGAA AATGAAGTTA AACAATATGT TCATCAAACA TCATGGGGTT GTACCACTAG ATCTATTGGT ATTATGATAA TGACACATGG TGATGATAAA GGATTAGTTC TACCACCAAA TGTTTCTAAA TATAAAGTTG TTATTGTACC TATCTTCTAT AAAACAACAG ATGAAAATGC TATACACTCA TATTGTAAAG ATATTGAAAA AATATTGAAA AATGCACAAA TTAATTGTGT TTATGATGAT AGAGCATCTT ATTCTCCTGG TTATAAATTC AATCATTGGG AATTAAGAGG TATTCCCATA AGAATAGAAG TTGGACCTAA AGATTTACAA AATAATTCTT GTGTTATTGT TAGAAGAGAT AATAACGAAA AATGTAATGT CAAAAAAGAA TCCGTATTAT TAGAAACACA ACAAATGCTT GTTGATATTC ATAAAAACCT CTTCTTAAAA GCAAAGAAAA AATTAGACGA TTCAATTGTT CAAGTTACGA GTTTTAGTGA AGTCATGAAT GCATTAAATA AAAAGAAAAT GGTACTTGCA CCATGGTGTG AAGATATTGC AACAGAAGAA GAAATTAAAA AAGAAACTCA AAGATTATCA TTAAACCAAA CAAACAGTGA AACGACATTA TCTGGTGCTA TGAAACCTTT ATGTATCCCG TTAGATCAAC CACCTATGCC ACCAAATATG AAATGTTTCT GGTCAGGGAA ACCAGCTAAA AGATGGTGTT TATTTGGTAG AAGTTATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for PlfaA.18681.a.B4.PW37539
PURIFICATION DATe: data unavailable
CONCENTRATION: data unavailable
OBSERVED MW: 60kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 110µl
PERCENT IDENTITY: 80
PERCENT COVERAGE: 80
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS NILGITSKKI ENFSDWYTQV IVKSELIEYY DISGCYILRP AAYYIWECVQ AFFNKEIKKL NVENSYFPLF VTKNKLEKEK NHIEGFSPEV AWVTKYGDSN LPEEIAIRPT SETIMYSVFP KWIRSYRDLP LKLNQWNTVV RWEFKQPTPF IRTREFLWQE GHTAHKNEEE AVKLVFDILD LYRRWYEEYL AVPIIKGIKS EGEKFGGANF TSTAEAFISE NGRAIQAATS HYLGTNFAKM FKIEFEDENE VKQYVHQTSW GCTTRSIGIM IMTHGDDKGX VLPPNVSKYK VVIVPIFYKT TDENAIXSYX XXXKNXENAX IXXVXXDXAX XXXGXXSXXX XXXXXXXXSX XXXXXXXXXX XXXXKXXXXX XXXXXKPXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgagtaa tattttaggt atcacttcaa aaaaaataga aaatttttca gattggtata ctcaagttat tgtaaaaagt gaattaattg aatattatga tatttcgggt tgttatatat taagacccgc tgcatattat atatgggaat gtgttcaagc tttttttaat aaagaaataa aaaaattaaa tgttgaaaat tcttatttcc cattatttgt tacaaaaaat aaattagaaa aagaaaaaaa tcatatagaa ggatttagtc cagaagttgc ttgggttaca aaatatggag attctaattt acctgaagaa attgctataa gaccaacaag tgaaacaatt atgtattcag tgtttccaaa atggattcga tcgtatcgtg atttacctct taaattaaat caatggaata ctgtggtacg ttgggaattt aaacaaccaa ctccatttat tagaactcga gaatttttat ggcaagaagg acatacagct cacaaaaatg aagaagaagc tgtaaaatta gtctttgata ttctagattt gtataggaga tggtatgaag aatatctagc tgtacctatt attaaaggaa taaaaagtga aggagaaaaa tttggaggtg caaattttac ttctactgct gaagctttta taagcgaaaa tggaagagcc atacaagcag ctacatctca ttatcttgga acaaattttg caaaaatgtt taaaattgaa tttgaagatg aaaatgaagt taaacaatat gttcatcaaa catcatgggg ttgtaccact agatctattg gtattatgat aatgacacat ggtgatgata aaggantagt tctaccacca aatgtttcta aatataaagt tgttattgta cctatcttct ataaaacaac agatgaaaat gctatacnct catattgnaa natnntgaaa aatantgaaa atgcacnaat tnanngtgtt natgangatn gagcannntn tnnnncnggt nnnaantcan cntnggnana nangnnntnc cnnnanaann naagtnnacn aanattnnna aannnnnntn nnnnnnnnnn nnnnnnannn aaangnnang nnnannnnan ncnnnntnnn tnnaaaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnan cannnannnn nnnnnnnnnn nnnnnnnt
Expressed Protein Sequence
MAHHHHHHSN ILGITSKKIE NFSDWYTQVI VKSELIEYYD ISGCYILRPA AYYIWECVQA FFNKEIKKLN VENSYFPLFV TKNKLEKEKN HIEGFSPEVA WVTKYGDSNL PEEIAIRPTS ETIMYSVFPK WIRSYRDLPL KLNQWNTVVR WEFKQPTPFI RTREFLWQEG HTAHKNEEEA VKLVFDILDL YRRWYEEYLA VPIIKGIKSE GEKFGGANFT STAEAFISEN GRAIQAATSH YLGTNFAKMF KIEFEDENEV KQYVHQTSWG CTTRSIGIMI MTHGDDKGLV LPPNVSKYKV VIVPIFYKTT DENAIHSYCK DIEKILKNAQ INCVYDDRAS YSPGYKFNHW ELRGIPIRIE VGPKDLQNNS CVIVRRDNNE KCNVKKESVL LETQQMLVDI HKNLFLKAKK KLDDSIVQVT SFSEVMNALN KKKMVLAPWC EDIATEEEIK KETQRLSLNQ TNSETTLSGA MKPLCIPLDQ PPMPPNMKCF WSGKPAKRWC LFGRSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAGTAATA TTTTAGGTAT CACTTCAAAA AAAATAGAAA ATTTTTCAGA TTGGTATACT CAAGTTATTG TAAAAAGTGA ATTAATTGAA TATTATGATA TTTCGGGTTG TTATATATTA AGACCCGCTG CATATTATAT ATGGGAATGT GTTCAAGCTT TTTTTAATAA AGAAATAAAA AAATTAAATG TTGAAAATTC TTATTTCCCA TTATTTGTTA CAAAAAATAA ATTAGAAAAA GAAAAAAATC ATATAGAAGG ATTTAGTCCA GAAGTTGCTT GGGTTACAAA ATATGGAGAT TCTAATTTAC CTGAAGAAAT TGCTATAAGA CCAACAAGTG AAACAATTAT GTATTCAGTG TTTCCAAAAT GGATTCGATC GTATCGTGAT TTACCTCTTA AATTAAATCA ATGGAATACT GTGGTACGTT GGGAATTTAA ACAACCAACT CCATTTATTA GAACTCGAGA ATTTTTATGG CAAGAAGGAC ATACAGCTCA CAAAAATGAA GAAGAAGCTG TAAAATTAGT CTTTGATATT CTAGATTTGT ATAGGAGATG GTATGAAGAA TATCTAGCTG TACCTATTAT TAAAGGAATA AAAAGTGAAG GAGAAAAATT TGGAGGTGCA AATTTTACTT CTACTGCTGA AGCTTTTATA AGCGAAAATG GAAGAGCCAT ACAAGCAGCT ACATCTCATT ATCTTGGAAC AAATTTTGCA AAAATGTTTA AAATTGAATT TGAAGATGAA AATGAAGTTA AACAATATGT TCATCAAACA TCATGGGGTT GTACCACTAG ATCTATTGGT ATTATGATAA TGACACATGG TGATGATAAA GGATTAGTTC TACCACCAAA TGTTTCTAAA TATAAAGTTG TTATTGTACC TATCTTCTAT AAAACAACAG ATGAAAATGC TATACACTCA TATTGTAAAG ATATTGAAAA AATATTGAAA AATGCACAAA TTAATTGTGT TTATGATGAT AGAGCATCTT ATTCTCCTGG TTATAAATTC AATCATTGGG AATTAAGAGG TATTCCCATA AGAATAGAAG TTGGACCTAA AGATTTACAA AATAATTCTT GTGTTATTGT TAGAAGAGAT AATAACGAAA AATGTAATGT CAAAAAAGAA TCCGTATTAT TAGAAACACA ACAAATGCTT GTTGATATTC ATAAAAACCT CTTCTTAAAA GCAAAGAAAA AATTAGACGA TTCAATTGTT CAAGTTACGA GTTTTAGTGA AGTCATGAAT GCATTAAATA AAAAGAAAAT GGTACTTGCA CCATGGTGTG AAGATATTGC AACAGAAGAA GAAATTAAAA AAGAAACTCA AAGATTATCA TTAAACCAAA CAAACAGTGA AACGACATTA TCTGGTGCTA TGAAACCTTT ATGTATCCCG TTAGATCAAC CACCTATGCC ACCAAATATG AAATGTTTCT GGTCAGGGAA ACCAGCTAAA AGATGGTGTT TATTTGGTAG AAGTTATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for PlfaA.18681.a.B4.PW37539
PURIFICATION DATe: data unavailable
CONCENTRATION: data unavailable
OBSERVED MW: 60kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 110µl
PERCENT IDENTITY: 80
PERCENT COVERAGE: 80
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS NILGITSKKI ENFSDWYTQV IVKSELIEYY DISGCYILRP AAYYIWECVQ AFFNKEIKKL NVENSYFPLF VTKNKLEKEK NHIEGFSPEV AWVTKYGDSN LPEEIAIRPT SETIMYSVFP KWIRSYRDLP LKLNQWNTVV RWEFKQPTPF IRTREFLWQE GHTAHKNEEE AVKLVFDILD LYRRWYEEYL AVPIIKGIKS EGEKFGGANF TSTAEAFISE NGRAIQAATS HYLGTNFAKM FKIEFEDENE VKQYVHQTSW GCTTRSIGIM IMTHGDDKGX VLPPNVSKYK VVIVPIFYKT TDENAIXSYX XXXKNXENAX IXXVXXDXAX XXXGXXSXXX XXXXXXXXSX XXXXXXXXXX XXXXKXXXXX XXXXXKPXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgagtaa tattttaggt atcacttcaa aaaaaataga aaatttttca gattggtata ctcaagttat tgtaaaaagt gaattaattg aatattatga tatttcgggt tgttatatat taagacccgc tgcatattat atatgggaat gtgttcaagc tttttttaat aaagaaataa aaaaattaaa tgttgaaaat tcttatttcc cattatttgt tacaaaaaat aaattagaaa aagaaaaaaa tcatatagaa ggatttagtc cagaagttgc ttgggttaca aaatatggag attctaattt acctgaagaa attgctataa gaccaacaag tgaaacaatt atgtattcag tgtttccaaa atggattcga tcgtatcgtg atttacctct taaattaaat caatggaata ctgtggtacg ttgggaattt aaacaaccaa ctccatttat tagaactcga gaatttttat ggcaagaagg acatacagct cacaaaaatg aagaagaagc tgtaaaatta gtctttgata ttctagattt gtataggaga tggtatgaag aatatctagc tgtacctatt attaaaggaa taaaaagtga aggagaaaaa tttggaggtg caaattttac ttctactgct gaagctttta taagcgaaaa tggaagagcc atacaagcag ctacatctca ttatcttgga acaaattttg caaaaatgtt taaaattgaa tttgaagatg aaaatgaagt taaacaatat gttcatcaaa catcatgggg ttgtaccact agatctattg gtattatgat aatgacacat ggtgatgata aaggantagt tctaccacca aatgtttcta aatataaagt tgttattgta cctatcttct ataaaacaac agatgaaaat gctatacnct catattgnaa natnntgaaa aatantgaaa atgcacnaat tnanngtgtt natgangatn gagcannntn tnnnncnggt nnnaantcan cntnggnana nangnnntnc cnnnanaann naagtnnacn aanattnnna aannnnnntn nnnnnnnnnn nnnnnnannn aaangnnang nnnannnnan ncnnnntnnn tnnaaaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnan cannnannnn nnnnnnnnnn nnnnnnnt
Expressed Protein Sequence
MAHHHHHHSN ILGITSKKIE NFSDWYTQVI VKSELIEYYD ISGCYILRPA AYYIWECVQA FFNKEIKKLN VENSYFPLFV TKNKLEKEKN HIEGFSPEVA WVTKYGDSNL PEEIAIRPTS ETIMYSVFPK WIRSYRDLPL KLNQWNTVVR WEFKQPTPFI RTREFLWQEG HTAHKNEEEA VKLVFDILDL YRRWYEEYLA VPIIKGIKSE GEKFGGANFT STAEAFISEN GRAIQAATSH YLGTNFAKMF KIEFEDENEV KQYVHQTSWG CTTRSIGIMI MTHGDDKGLV LPPNVSKYKV VIVPIFYKTT DENAIHSYCK DIEKILKNAQ INCVYDDRAS YSPGYKFNHW ELRGIPIRIE VGPKDLQNNS CVIVRRDNNE KCNVKKESVL LETQQMLVDI HKNLFLKAKK KLDDSIVQVT SFSEVMNALN KKKMVLAPWC EDIATEEEIK KETQRLSLNQ TNSETTLSGA MKPLCIPLDQ PPMPPNMKCF WSGKPAKRWC LFGRSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAGTAATA TTTTAGGTAT CACTTCAAAA AAAATAGAAA ATTTTTCAGA TTGGTATACT CAAGTTATTG TAAAAAGTGA ATTAATTGAA TATTATGATA TTTCGGGTTG TTATATATTA AGACCCGCTG CATATTATAT ATGGGAATGT GTTCAAGCTT TTTTTAATAA AGAAATAAAA AAATTAAATG TTGAAAATTC TTATTTCCCA TTATTTGTTA CAAAAAATAA ATTAGAAAAA GAAAAAAATC ATATAGAAGG ATTTAGTCCA GAAGTTGCTT GGGTTACAAA ATATGGAGAT TCTAATTTAC CTGAAGAAAT TGCTATAAGA CCAACAAGTG AAACAATTAT GTATTCAGTG TTTCCAAAAT GGATTCGATC GTATCGTGAT TTACCTCTTA AATTAAATCA ATGGAATACT GTGGTACGTT GGGAATTTAA ACAACCAACT CCATTTATTA GAACTCGAGA ATTTTTATGG CAAGAAGGAC ATACAGCTCA CAAAAATGAA GAAGAAGCTG TAAAATTAGT CTTTGATATT CTAGATTTGT ATAGGAGATG GTATGAAGAA TATCTAGCTG TACCTATTAT TAAAGGAATA AAAAGTGAAG GAGAAAAATT TGGAGGTGCA AATTTTACTT CTACTGCTGA AGCTTTTATA AGCGAAAATG GAAGAGCCAT ACAAGCAGCT ACATCTCATT ATCTTGGAAC AAATTTTGCA AAAATGTTTA AAATTGAATT TGAAGATGAA AATGAAGTTA AACAATATGT TCATCAAACA TCATGGGGTT GTACCACTAG ATCTATTGGT ATTATGATAA TGACACATGG TGATGATAAA GGATTAGTTC TACCACCAAA TGTTTCTAAA TATAAAGTTG TTATTGTACC TATCTTCTAT AAAACAACAG ATGAAAATGC TATACACTCA TATTGTAAAG ATATTGAAAA AATATTGAAA AATGCACAAA TTAATTGTGT TTATGATGAT AGAGCATCTT ATTCTCCTGG TTATAAATTC AATCATTGGG AATTAAGAGG TATTCCCATA AGAATAGAAG TTGGACCTAA AGATTTACAA AATAATTCTT GTGTTATTGT TAGAAGAGAT AATAACGAAA AATGTAATGT CAAAAAAGAA TCCGTATTAT TAGAAACACA ACAAATGCTT GTTGATATTC ATAAAAACCT CTTCTTAAAA GCAAAGAAAA AATTAGACGA TTCAATTGTT CAAGTTACGA GTTTTAGTGA AGTCATGAAT GCATTAAATA AAAAGAAAAT GGTACTTGCA CCATGGTGTG AAGATATTGC AACAGAAGAA GAAATTAAAA AAGAAACTCA AAGATTATCA TTAAACCAAA CAAACAGTGA AACGACATTA TCTGGTGCTA TGAAACCTTT ATGTATCCCG TTAGATCAAC CACCTATGCC ACCAAATATG AAATGTTTCT GGTCAGGGAA ACCAGCTAAA AGATGGTGTT TATTTGGTAG AAGTTATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for PlfaA.18681.a.B4.PW38348
PURIFICATION DATe: 9/4/2017
CONCENTRATION: 20.9mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 80
PERCENT COVERAGE: 80
Protocol Notes
Combined with 43281 IMAC->SEC
Validated AA Sequence
MAHHHHHHMS NILGITSKKI ENFSDWYTQV IVKSELIEYY DISGCYILRP AAYYIWECVQ AFFNKEIKKL NVENSYFPLF VTKNKLEKEK NHIEGFSPEV AWVTKYGDSN LPEEIAIRPT SETIMYSVFP KWIRSYRDLP LKLNQWNTVV RWEFKQPTPF IRTREFLWQE GHTAHKNEEE AVKLVFDILD LYRRWYEEYL AVPIIKGIKS EGEKFGGANF TSTAEAFISE NGRAIQAATS HYLGTNFAKM FKIEFEDENE VKQYVHQTSW GCTTRSIGIM IMTHGDDKGX VLPPNVSKYK VVIVPIFYKT TDENAIXSYX XXXKNXENAX IXXVXXDXAX XXXGXXSXXX XXXXXXXXSX XXXXXXXXXX XXXXKXXXXX XXXXXKPXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgagtaa tattttaggt atcacttcaa aaaaaataga aaatttttca gattggtata ctcaagttat tgtaaaaagt gaattaattg aatattatga tatttcgggt tgttatatat taagacccgc tgcatattat atatgggaat gtgttcaagc tttttttaat aaagaaataa aaaaattaaa tgttgaaaat tcttatttcc cattatttgt tacaaaaaat aaattagaaa aagaaaaaaa tcatatagaa ggatttagtc cagaagttgc ttgggttaca aaatatggag attctaattt acctgaagaa attgctataa gaccaacaag tgaaacaatt atgtattcag tgtttccaaa atggattcga tcgtatcgtg atttacctct taaattaaat caatggaata ctgtggtacg ttgggaattt aaacaaccaa ctccatttat tagaactcga gaatttttat ggcaagaagg acatacagct cacaaaaatg aagaagaagc tgtaaaatta gtctttgata ttctagattt gtataggaga tggtatgaag aatatctagc tgtacctatt attaaaggaa taaaaagtga aggagaaaaa tttggaggtg caaattttac ttctactgct gaagctttta taagcgaaaa tggaagagcc atacaagcag ctacatctca ttatcttgga acaaattttg caaaaatgtt taaaattgaa tttgaagatg aaaatgaagt taaacaatat gttcatcaaa catcatgggg ttgtaccact agatctattg gtattatgat aatgacacat ggtgatgata aaggantagt tctaccacca aatgtttcta aatataaagt tgttattgta cctatcttct ataaaacaac agatgaaaat gctatacnct catattgnaa natnntgaaa aatantgaaa atgcacnaat tnanngtgtt natgangatn gagcannntn tnnnncnggt nnnaantcan cntnggnana nangnnntnc cnnnanaann naagtnnacn aanattnnna aannnnnntn nnnnnnnnnn nnnnnnannn aaangnnang nnnannnnan ncnnnntnnn tnnaaaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnan cannnannnn nnnnnnnnnn nnnnnnnt
Expressed Protein Sequence
MAHHHHHHSN ILGITSKKIE NFSDWYTQVI VKSELIEYYD ISGCYILRPA AYYIWECVQA FFNKEIKKLN VENSYFPLFV TKNKLEKEKN HIEGFSPEVA WVTKYGDSNL PEEIAIRPTS ETIMYSVFPK WIRSYRDLPL KLNQWNTVVR WEFKQPTPFI RTREFLWQEG HTAHKNEEEA VKLVFDILDL YRRWYEEYLA VPIIKGIKSE GEKFGGANFT STAEAFISEN GRAIQAATSH YLGTNFAKMF KIEFEDENEV KQYVHQTSWG CTTRSIGIMI MTHGDDKGLV LPPNVSKYKV VIVPIFYKTT DENAIHSYCK DIEKILKNAQ INCVYDDRAS YSPGYKFNHW ELRGIPIRIE VGPKDLQNNS CVIVRRDNNE KCNVKKESVL LETQQMLVDI HKNLFLKAKK KLDDSIVQVT SFSEVMNALN KKKMVLAPWC EDIATEEEIK KETQRLSLNQ TNSETTLSGA MKPLCIPLDQ PPMPPNMKCF WSGKPAKRWC LFGRSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAGTAATA TTTTAGGTAT CACTTCAAAA AAAATAGAAA ATTTTTCAGA TTGGTATACT CAAGTTATTG TAAAAAGTGA ATTAATTGAA TATTATGATA TTTCGGGTTG TTATATATTA AGACCCGCTG CATATTATAT ATGGGAATGT GTTCAAGCTT TTTTTAATAA AGAAATAAAA AAATTAAATG TTGAAAATTC TTATTTCCCA TTATTTGTTA CAAAAAATAA ATTAGAAAAA GAAAAAAATC ATATAGAAGG ATTTAGTCCA GAAGTTGCTT GGGTTACAAA ATATGGAGAT TCTAATTTAC CTGAAGAAAT TGCTATAAGA CCAACAAGTG AAACAATTAT GTATTCAGTG TTTCCAAAAT GGATTCGATC GTATCGTGAT TTACCTCTTA AATTAAATCA ATGGAATACT GTGGTACGTT GGGAATTTAA ACAACCAACT CCATTTATTA GAACTCGAGA ATTTTTATGG CAAGAAGGAC ATACAGCTCA CAAAAATGAA GAAGAAGCTG TAAAATTAGT CTTTGATATT CTAGATTTGT ATAGGAGATG GTATGAAGAA TATCTAGCTG TACCTATTAT TAAAGGAATA AAAAGTGAAG GAGAAAAATT TGGAGGTGCA AATTTTACTT CTACTGCTGA AGCTTTTATA AGCGAAAATG GAAGAGCCAT ACAAGCAGCT ACATCTCATT ATCTTGGAAC AAATTTTGCA AAAATGTTTA AAATTGAATT TGAAGATGAA AATGAAGTTA AACAATATGT TCATCAAACA TCATGGGGTT GTACCACTAG ATCTATTGGT ATTATGATAA TGACACATGG TGATGATAAA GGATTAGTTC TACCACCAAA TGTTTCTAAA TATAAAGTTG TTATTGTACC TATCTTCTAT AAAACAACAG ATGAAAATGC TATACACTCA TATTGTAAAG ATATTGAAAA AATATTGAAA AATGCACAAA TTAATTGTGT TTATGATGAT AGAGCATCTT ATTCTCCTGG TTATAAATTC AATCATTGGG AATTAAGAGG TATTCCCATA AGAATAGAAG TTGGACCTAA AGATTTACAA AATAATTCTT GTGTTATTGT TAGAAGAGAT AATAACGAAA AATGTAATGT CAAAAAAGAA TCCGTATTAT TAGAAACACA ACAAATGCTT GTTGATATTC ATAAAAACCT CTTCTTAAAA GCAAAGAAAA AATTAGACGA TTCAATTGTT CAAGTTACGA GTTTTAGTGA AGTCATGAAT GCATTAAATA AAAAGAAAAT GGTACTTGCA CCATGGTGTG AAGATATTGC AACAGAAGAA GAAATTAAAA AAGAAACTCA AAGATTATCA TTAAACCAAA CAAACAGTGA AACGACATTA TCTGGTGCTA TGAAACCTTT ATGTATCCCG TTAGATCAAC CACCTATGCC ACCAAATATG AAATGTTTCT GGTCAGGGAA ACCAGCTAAA AGATGGTGTT TATTTGGTAG AAGTTATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT