PlfaA.18558.a

Dihydrofolate synthase/folylpolyglutamate synthase (EC 6.3.2.12)

CENTER ID: PlfaA.18558.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18558.a.B1.GE37856 full length 1 522
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1324800
RefSeq: XP_001349988.1
UniProt: Q8IE69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKNQNDKSN KNDIIHMNDK SGNYDKNNIN NFIDKNDEHD MSDILHKINN EEKKYEEIKS YSECLELLYK THALKLGLDN PKKLNESFGH PCDKYKTIHI AGTNGKGSVC YKIYTCLKIK KFKVGLFSSP HIFSLRERII VNDEPISEKE LIHLVNEVLN KAKKLYINPS FFEIITLVAF LHFLNKKVDY AIIETGIGGR LDATNILTKP EVIVITSIGY DHLNILGDNL PIICNEKIGI FKKDANVVIG PSVAIYKNVF DKAKELNCTI HTVVPEPRGE RYNEENSRIA LRTLEILNIS IDYFLKSIIP IKPPLRIQYL ATEQIQHIKK KFSPDNLEHN VQYPLAVILD VGHNETAIDR LCTDINYFHK GQNIRICISI TKPRNLSVFH PFIAQFGDTL KDIFYLPSLN ERTYDFEEIV EMLNNEEEIK NEIKELILSS SKKVGKWLAH EKQGNINEED ALKLYKRGCI PLIIKNAFLE CCKDNSILLV CGTFFVFDEV LNVFDIHSDM QDTIFMNEPS LV
NT Sequence
atggaaaaaa atcaaaatga taaaagtaac aaaaatgata taattcacat gaatgataaa agtggaaatt atgataaaaa taatataaat aattttattg ataaaaatga tgagcatgat atgagtgaca tattacataa aataaataat gaggaaaaaa aatatgaaga aataaaatct tacagtgagt gcttagaatt attatataaa acacatgccc taaaattagg acttgataac ccaaaaaaat tgaacgaatc ttttggtcac ccttgtgata aatataaaac tattcatatt gcagggacaa atgggaaagg gtctgtatgc tataaaatat atacatgtct taaaataaaa aaattcaagg tgggtctttt ttcatcacct catatatttt ctttaagaga aagaattata gtaaacgatg aaccaataag tgaaaaagag ttaatacatt tagtaaacga agtattaaat aaagccaaaa aattatatat aaatccatct ttttttgaaa taattacatt agttgcattt ttacattttt taaataagaa ggtagattat gctataatag aaacagggat tggagggcgc ttagatgcaa ctaatatatt aaccaaacca gaagttattg taattacttc cataggatat gatcatttaa atatattagg tgataatttg cctattatat gtaatgaaaa aattggaatt tttaaaaaag atgctaacgt tgtaatagga ccatcagtag ctatttataa aaatgttttt gataaggcaa aagaattaaa ttgtactata catactgtag tacctgaacc acgaggagaa agatataatg aagaaaattc aagaatagca ttgcgcactt tagaaatatt aaatataagt atagattact ttttaaagtc cattatacca attaaaccac ctttaagaat tcaatattta gctaccgagc aaatacaaca tataaagaaa aaattttcac cagataattt agaacacaat gtacaatatc ctctagctgt tattttagat gtaggccaca atgaaacggc aatagataga ttgtgtaccg atattaatta ttttcataag ggacaaaata taaggatatg tatatctata acaaagccga gaaacttgag tgtattccat ccgttcatag ctcaatttgg cgacacctta aaggatatat tttaccttcc atcattaaat gaaaggacct acgactttga ggaaatcgtt gagatgttaa ataatgaaga agaaataaaa aatgaaataa aagagctcat attaagtagc tctaaaaagg ttggtaaatg gttagctcat gagaaacaag gaaatattaa tgaagaagat gcacttaaat tatacaaaag aggatgcata ccattaataa tcaaaaatgc atttcttgaa tgttgcaaag acaatagtat attattagta tgtgggacgt ttttcgtgtt tgatgaggtt ttaaacgttt ttgatattca ctctgatatg caagatacca tttttatgaa tgaaccatct cttgtttaa
Details for PlfaA.18558.a.B1.GE37856
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 61kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 57
Validated AA Sequence
MNDKSGNYDK NNINNFIDKN DEHDMSDILH KINNEEKKYE EIKSYSECLE LLYKTHALKL GLDNPKKLNE SFGHPCDKYK TIHIAGTNGK GSVCYKIYTC LKIKKFKVGL FSSPHIFSLR ERIIVNDEPI SEKELIHLVN EVLNKAKKLY INPSFFEIIT LVAFLHFLNK KVDYAIIETG IGGRLDATNI LTKPEVIVIT SIGYDHLNIL GDNLPIICNE KIGIFKKDAN VVIGPSVAIY KNVFDKAKEL NCTIHTVVPE PRGERYNEEN SRIALRTLEI LNISIDYFLK SIIPIKPPLR IHI
Validated NT Sequence
aatggtatct tgcatatcag agtgaatatc aaaaacgttt aaaacctcat caaacacgaa aaacgtccca catactaata atatactatt gtctttgcaa cattcaagaa atgcattttt gattattaat ggtatgcatc ctcttttgta taatttaagt gcatcttctt cattaatatt tccttgtttc tcatgagcta accatttacc aaccttttta gagctactta atatgagctc ttttatttca ttttttattt cttcttcatt atttaacatc tcaacgattt cctcaaagtc gtaggtcctt tcatttaatg atggaaggta aaatatatcc tttaaggtgt cgccaaattg anctatgaac ggatggaata cactcaagtt tctcggcttt gttatagata tacatatcct tatattttgt cccttatgaa aataattaat atcggtacac aatctatcta ttgccgtttc attgtggcct acatctaaaa taacagctag aggatattgt acattgtgtt ctaaattatc tggtgaaaat tttttcttta tatgttgtat ttgctcggta gctaaatatg aattcttaaa ggtggtttaa ttggtataat ggactttaaa aagtaatcta tacttatatt taatatttct aaagtgcgca atgctattct tgaattttct tcattatatc tttctcctcg tggttcaggt actacagtat gtatagtaca atttaattct tttgccttat caaaaacatt tttataaata gctactgatg gtcctattac aacgttagca tcttttttaa aaattccaat tttttcatta catataatag gcaaattatc acctaatata tttaaatgat catatcctat ggaagtaatt acaataactt ctggtttggt taatatatta gttgcatcta agcgccctcc aatccctgtt tctattatag cataatctac cttcttattt aaaaaatgta aaaatgcaac taatgtaatt atttcaaaaa aagatggatt tatatataat tttttggctt tatttaatac ttcgtttact aaatgtatta actctttttc acttattggt tcatcgttta ctataattct ttctcttaaa gaaaatatat gaggtgatga aaaaagaccc accttgaatt tttttatttt aagacatgta tatattttat agcatacaga ccctttccca tttgtccctg caatatgaat agttttatat ttatcacaag ggtgaccaaa agattcgttc aatttttttg ggttatcaag tcctaatttt agggcatgtg ttttatataa taattctaag cactcactgt aagattttat ttcttcatat tttttttcct cattatttat tttatgtaat atgtcactca tatcatgctc atcattttta tcaataaaat tatttatatt atttttatca taatttccac ttttatcatt catgtgaatt atatcatttt tgttactttt atcattttga tttttt
Expected Protein Sequence
MAHHHHHHME KNQNDKSNKN DIIHMNDKSG NYDKNNINNF IDKNDEHDMS DILHKINNEE KKYEEIKSYS ECLELLYKTH ALKLGLDNPK KLNESFGHPC DKYKTIHIAG TNGKGSVCYK IYTCLKIKKF KVGLFSSPHI FSLRERIIVN DEPISEKELI HLVNEVLNKA KKLYINPSFF EIITLVAFLH FLNKKVDYAI IETGIGGRLD ATNILTKPEV IVITSIGYDH LNILGDNLPI ICNEKIGIFK KDANVVIGPS VAIYKNVFDK AKELNCTIHT VVPEPRGERY NEENSRIALR TLEILNISID YFLKSIIPIK PPLRIQYLAT EQIQHIKKKF SPDNLEHNVQ YPLAVILDVG HNETAIDRLC TDINYFHKGQ NIRICISITK PRNLSVFHPF IAQFGDTLKD IFYLPSLNER TYDFEEIVEM LNNEEEIKNE IKELILSSSK KVGKWLAHEK QGNINEEDAL KLYKRGCIPL IIKNAFLECC KDNSILLVCG TFFVFDEVLN VFDIHSDMQD TIFMNEPSLV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aaaatcaaaa tgataaaagt aacaaaaatg atataattca catgaatgat aaaagtggaa attatgataa aaataatata aataatttta ttgataaaaa tgatgagcat gatatgagtg acatattaca taaaataaat aatgaggaaa aaaaatatga agaaataaaa tcttacagtg agtgcttaga attattatat aaaacacatg ccctaaaatt aggacttgat aacccaaaaa aattgaacga atcttttggt cacccttgtg ataaatataa aactattcat attgcaggga caaatgggaa agggtctgta tgctataaaa tatatacatg tcttaaaata aaaaaattca aggtgggtct tttttcatca cctcatatat tttctttaag agaaagaatt atagtaaacg atgaaccaat aagtgaaaaa gagttaatac atttagtaaa cgaagtatta aataaagcca aaaaattata tataaatcca tctttttttg aaataattac attagttgca tttttacatt ttttaaataa gaaggtagat tatgctataa tagaaacagg gattggaggg cgcttagatg caactaatat attaaccaaa ccagaagtta ttgtaattac ttccatagga tatgatcatt taaatatatt aggtgataat ttgcctatta tatgtaatga aaaaattgga atttttaaaa aagatgctaa cgttgtaata ggaccatcag tagctattta taaaaatgtt tttgataagg caaaagaatt aaattgtact atacatactg tagtacctga accacgagga gaaagatata atgaagaaaa ttcaagaata gcattgcgca ctttagaaat attaaatata agtatagatt actttttaaa gtccattata ccaattaaac cacctttaag aattcaatat ttagctaccg agcaaataca acatataaag aaaaaatttt caccagataa tttagaacac aatgtacaat atcctctagc tgttatttta gatgtaggcc acaatgaaac ggcaatagat agattgtgta ccgatattaa ttattttcat aagggacaaa atataaggat atgtatatct ataacaaagc cgagaaactt gagtgtattc catccgttca tagctcaatt tggcgacacc ttaaaggata tattttacct tccatcatta aatgaaagga cctacgactt tgaggaaatc gttgagatgt taaataatga agaagaaata aaaaatgaaa taaaagagct catattaagt agctctaaaa aggttggtaa atggttagct catgagaaac aaggaaatat taatgaagaa gatgcactta aattatacaa aagaggatgc ataccattaa taatcaaaaa tgcatttctt gaatgttgca aagacaatag tatattatta gtatgtggga cgtttttcgt gtttgatgag gttttaaacg tttttgatat tcactctgat atgcaagata ccatttttat gaatgaacca tctcttgttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat