PlfaA.18519.a

Erythrocyte membrane protein 1

CENTER ID: PlfaA.18519.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18519.a.B21.GE37683 NTSB3-DBLa2-VVV domain 1 484
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18519.a.B21.PW37469 NTSB3-DBLa2-VVV domain 1 484

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PfIT_010005000
UniProt: A3R6S3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGPKAAVTDY SDAKHLLDSI GEKVYKEKVE KKAVDYRSAL QGRLSEASFK DRKNIERGKA ELCKLNHIYH TNVTDGYGRE HPCKDRWDIR FSDKYGGQCT NSKIHGNDDS NGKDIGACAP FRRLHLCDQH LSHMKAEKIN SKDNLLLEVC LAAQYEGESL VEKHKEFKKT HNDSNICTIL ARSFADIGDI IRGKDLYLGN KKEKEKLENN LKKIFKEIYK DVMKTSGSNG KTNGEIEARY GSDKDPNYYE LREDWWDANR REVWKAMTCN ADNDSQYFRQ TCGSREKGSA TKGKCRCVIG DVPTYFDYVP QYLRWFQEWA EDFCRIKKIK LELAKKACRD ESSKLYCSHN GYDCTKRIEK GKSCSRDSKC TECSTKCIPY EYWLEKQQNE FNMQKDKYDK EIRTKTSNNG ISNSNINNKY YKEFYNELNK KYESVENFLT LLNNGMYCKK HVDGESTINF NKTDDKGAFY RSDYCQPCPD CVVVCEGGNC KEKTEDDNCR SEIIKKILKY ETPTPIDVLY SGKGQGLITK KLEDFCSSTT NYEGTNVQKW KCYNKNKDYN NCEMNISSYK DATDPNVMLS VECFHSWAKN LLIDTIRWEH QLKNCINNTN VTDCTSKCIK NCECYEKWIK EKEKEWPQVK GVLKKKDETS HNYYDKLKDV FDRFLFEVKG ALDQDEKGKW DQFTKDLEKK FGPSVESAGT ANSQDAIELL LDHLNDNATT CKDNNSLAVE NCPKTKINPC IKGTRKRTPK RTGGASNNLV SVKHIAEIMQ QDAREQLEKR GGESNLKGDA SKGKYTKKNG KAVALNDICS IDVQHSNSTY RSVKPCAGKN TGRFDIGTPW SYGEKKKEMT HPKVYMPPRR EHMCTSNLEY LETGEGPLKG KDNNGKLVNN SFLGDVLLSA KKEGDFIVKK LGSDKSAICN AMKYSFADLG DIIRGRDMWD KDSGSKKMDV ILKNVFGTLH KSLEGIRNHP KYAYDKNKTS PYKQLREDWW EANRHQVWRA MKCEIKKDKN MKCNGIPIED YIPQRLRWMT EWAEWYCKAQ NKYYGELEKE CGGCMGNGQG CTSSDPKCKQ CKNACDAYKK KIETWEKQWE KIKGKYEELY KKALDSVNGN GKGEKSTTSG TKDEKDVVDF LKQLLAQNSA AARVRVIRAA GSPTEITAAA PITPYSTAAG YIHQELGKTV GCNIQTKFCK HKIGSKASGT ENKEYAFRDK PHDHDEACAC RPPSTPVDVS RALKPRGPAS EDSEDDEDED EVEEEEGTTP GPPATPVPAG PTVVDVCKIV DGILKGNDGS NEISGCNPKE SYPDWDCKKN IDNSHSGACM PPRRQKLCVR DLTQGGEIRK PEDILTKFIN CAAKETHFAW HKYKKDNVNA ENELKSGKIP EGFRKQMYYT FGDFRDIFFG TDISSCRYIK DTSQTIKSKL GDQATTEKGD THIDDNKKLQ EWWTIHGPKI WEGMLCALTN GLSESEKENI LQDYSYNKLN NAEKDDCCLE KFASKPQFLR WYVEWSDEFC RERKKLEDKV EDVCIKAKDY EGCKNNKSNN SCVKVCKEYE NYITGKKTQY ESQEGKFNTE KRQKKPEYNS YSKKDASEYL KDKCLDGTCD CMKKVKSIDN YWKNPHKTYD DNKLETKCEC PPTPPKPASK PDLARSDTYQ PPAPPKPRAG EGLGRSLPNE PRDTIEEDDD DDDEDEDDEG KVEAAKEDQV DRKGETAKEE EPPASQNDVN VCKTVEEALK GKLDDACTLK YSGNNSRLGW KCIPSGKPGD TGSSGAICVP PRRRRLYVTP LTKWAETVAQ PQESGGNTES SDKLREAFIQ SAAVETFFLW HKFKKEKERE EKEKKEANGE LPGFSSSVDG GEQNPQTLLQ NGTIPPDFLR LMFYTLGDYR DICVGKTPDG IDTVSGKDTM ENIKKAIESV FKPSSGTPSL SVEKTTRESW WEKNGEHIWK GMICALTYKE NGADGTNKIE KDSDVYKKIF GENNTDPDKK LTVTTVDNKG TFESKYKYEK VQLGEEEASG PNAPASPTSP QANGTRLAEF VTRPAYFRYL EEWGETFCKE RKKRLEKIKQ KCKVEEDEYK CSGFGEDCQT NLKNDPSTIP SLECPDCAKH CSSYRKWIGR KKYEFTEQQN AFTKQKTDAH NNNGFCGKEG KCNTAADFLE RLKNGPCKNN ENRTNDINFG DVNGETFKHA ENCNPCSEFK INCENGNCGG GTKVTCPGGK ITAQNMKDME KNTEINMLVS DDSTNGVEGD LKDCENEGIF EGIRKDEWIC GTVCGYNVCK PAKVNGQKDG EKHIITIRAL VTHWVHNFLE DYNRIKHRIS HCMEKGEPSN CINGCNKKCN CVKKWISTKK QEWQQIKKQY LEQYKNQGES YPTKTVLEEF KERPVLNKAI KPCGGLEKFQ NSTDCTVAGS SESGKDGTQK DIVECLLHKL ETKAKNCPGK PSAEEQKPCD VSNPLVEDDD EHFLEEEDQN PEENTVGKQQ PSFCPPVEDK KKEEEEETCT PASPVPEKPV PQVPCWRPFK PPNMFPKRIK KKNACEIAEE ILKDNDGKKQ VGQCHPKNND KNYLQWNCDE NKIKTTEVGA CMPPRRQKLC LYYLTQLNYN DNADKLREAF IKSAAAETFL SWHYFKTKNG DNAQTQLKAG KIPAEFLRSM FNTYGDYRDL FLEKDISSEV HTIKDNINKV FRNSVRRRGE TSDTKRQKWW NEHGPEIWKG MLCALSYDTE EKNVNTQIRQ RLTKYYDYKN VAFTYKSGTP LEKFASRPQF LRWFTEWGED FCTQRKKQFE ILQGKCVECT VSDSGTSDKT KTCDDKKNCD TCKTACTTYQ NWLQKWKTDY AKQRNKYAED KKKDLYKSID DVKNSPNAFE YLYKQSKHFI CENGDCNCME NSSKQQKQSS TDGDKMPESL DEKPEKVKDK CNCVLHECSG LSVTDSGIPD GSAFGGGLPP GKCKGLEGGP QKKIEPPTSD YINDILKSTI PVGIALALGS IAFLFMKKKP KTPVDLLRVL DIHKGDYGMP TLESKNRYIP YRSGTYKGKT YIYMEGDSDE DKYAFMSDTT DVTSSESEYE ELDINDIYVP GSPKYKTLIE VVLEPSKRDI PSGDIPNNNT PMNKFTDEEW NQLKHDFISQ YLPNTEPNTL YFDKPEEKPF ITSIHDRNLY TGEEYSYNIN MSTNTMDDPK YVSNNVYSGI DLINDSLNSG NQPIDIYDEV LKRKENELFG TEHHPKRTTT NHFATPTRDD PIHNQLELFH KWLDRHRDMC EKWNNKEEVL DKLKEEWENE THSGNTHPSD SNKTLNTNVS IQIDMDHEKR MKEFTNMDTY PENSTMDSIL EDLEKYKEPY YDVQDDIYYD VNDHDTSTVG SNNMDVPSKV QIEMDVNTKL VKEKYPISDI WNI
NT Sequence
atggggccca aggcggctgt gactgactac agtgatgcca aacatttatt ggatagcata ggggaaaaag tgtacaaaga aaaagtggaa aaaaaagctg tagattatag aagcgctttg caaggacgtt tgtcagaagc atcatttaaa gatcgtaaga acatcgagcg tggaaaagcc gaattatgta aacttaatca tatatatcat actaatgtta cggatggtta tggtagggag catccttgta aagatagatg ggacattcgc ttttctgata aatatggtgg tcaatgcact aatagtaaaa tacatggtaa tgatgatagt aatggtaaag acattggagc ctgcgcgccg ttcagacgat tacatctatg tgaccaacat ttatcgcaca tgaaagctga aaaaattaat tctaaagata atttgttgtt agaagtgtgt cttgcagcac aatatgaagg agaatcatta gtagaaaaac ataaagaatt taaaaaaaca cataacgatt ccaatatatg tactatattg gcacgaagtt ttgcagatat aggagatatt attagaggaa aagatctgta tcttggtaat aagaaagaaa aagagaaatt agaaaataat ttaaaaaaaa ttttcaagga aatatataag gacgtgatga agacgagcgg gagtaatggg aagacgaatg gggagataga agctcgctac ggaagtgata aagatccaaa ttattatgaa ttgagagaag attggtggga tgctaataga agagaggtat ggaaagcaat gacatgcaat gctgataatg attctcaata ttttcgacaa acatgtggtt caagagagaa gggaagtgcg actaaaggta aatgccgatg tgtgattgga gatgttccca catattttga ctacgtgccg cagtatcttc gctggttcca ggaatgggcc gaagactttt gtagaataaa aaaaattaaa ttagaattgg ccaagaaagc gtgtcgtgat gaatcatcaa aattatattg tagtcataat ggatatgatt gtacgaaacg tattgaaaaa ggaaaaagtt gctctaggga tagtaagtgt actgaatgtt cgactaaatg cattccttat gaatattggt tagagaaaca acaaaatgaa tttaacatgc aaaaggataa atatgataag gaaataagaa caaagacatc caacaacggt atatctaata gtaatattaa taacaaatat tataaggaat tttataacga acttaataaa aaatatgaaa gcgttgaaaa ttttttaacc ttactaaata atggaatgta ttgtaaaaaa catgtggatg gagaaagtac tattaatttt aataagactg atgataaagg tgcgttttat cgctcagatt attgccaacc ctgtcccgat tgtgtagttg tttgtgaggg tggaaattgt aaagaaaaaa cggaagatga caattgcagg agcgaaataa tcaaaaaaat tctaaaatat gagacgccta ctccaattga tgtcctctat agtggtaaag gacaaggtct tattacaaaa aaattagaag atttttgttc gagcacaact aattacgaag gtacaaatgt tcaaaaatgg aaatgctata ataaaaataa ggattataat aattgtgaaa tgaacatttc atcatacaaa gatgcaactg atcctaacgt tatgttatct gttgaatgtt ttcattcgtg ggctaaaaat ttattaatag acaccataag gtgggaacat caacttaaga attgcataaa taatactaat gtcacggatt gtacaagtaa atgtatcaaa aattgtgaat gttatgaaaa atggattaaa gaaaaagaaa aggagtggcc acaagtaaaa ggagtattaa aaaaaaaaga cgaaacatca cacaattatt atgataaact taaagatgtt tttgatcgtt ttttatttga agttaagggt gcgcttgacc aagacgaaaa aggaaaatgg gatcaattta cgaaagattt agaaaaaaaa tttggacctt ccgtagaaag tgcaggtacc gcaaattcgc aagatgcaat agaactttta ttagatcact taaatgataa tgccacaaca tgcaaagaca ataattcatt agcagttgaa aattgcccca aaactaaaat aaacccatgt ataaaaggca ctcgtaaacg cactcctaaa cgcactggtg gtgctagtaa caaccttgtg agtgtgaaac acatagccga aataatgcaa caggacgcaa gggaacaatt ggagaaacgt ggcggcgaaa gtaatttgaa aggtgatgca tcaaaaggga aatacacaaa aaaaaatggt aaggcagttg cgttgaatga catatgtagt atagatgtcc aacattccaa ttctacatat agatcggtaa aaccatgtgc aggaaaaaat acaggcaggt ttgacatagg aacgccatgg tcttatgggg agaagaaaaa agaaatgaca caccctaagg tttatatgcc accaagaaga gaacatatgt gtacatcaaa tttggaatat ttagaaacgg gtgaaggccc acttaaaggt aaggataata atggtaaact tgttaataat tcttttttgg gtgatgttct tctctcagca aaaaaggaag gagatttcat agtaaaaaaa cttggtagtg ataaatctgc aatttgtaat gctatgaaat atagtttcgc cgaccttgga gatattatac gaggaagaga catgtgggat aaagacagtg gctcaaaaaa aatggatgta attttgaaaa acgtttttgg tactctccat aagtcacttg aaggcatcag aaaccacccc aaatatgcct atgataaaaa caaaacctcc ccatataaac aattgagaga agactggtgg gaagctaata gacatcaggt gtggagagct atgaaatgtg aaataaaaaa ggacaagaat atgaaatgta atggtatccc aatagaagat tatattccgc aaagattaag atggatgaca gaatgggctg aatggtattg caaagcacaa aataaatatt atggggagtt ggagaaggag tgtgggggtt gtatgggtaa tggtcaaggt tgtacgagta gtgatccgaa gtgtaagcag tgcaaaaacg catgtgatgc atataaaaag aaaatagaaa catgggaaaa acaatgggaa aaaataaaag gaaaatacga agaattatac aaaaaagcat tagatagtgt taatggtaac ggaaagggtg aaaaaagtac tactagtggt accaaagatg aaaaagacgt tgttgatttc ttgaaacaat tactagcaca aaatagtgct gccgcacgtg ttcgtgttat acgtgctgct ggtagtccca ctgagatcac cgccgccgcc ccgatcaccc cttactccac cgccgcaggg tacatacatc aggaattggg gaagaccgtg gggtgcaaca tacaaacaaa attttgtaaa cacaaaattg gtagtaaagc tagtggtaca gaaaacaaag aatatgcctt tagagataaa ccacatgatc atgatgaggc gtgtgcttgc aggccaccat caacgccagt agacgtctcc cgagccctaa aaccacgtgg ccccgcctca gaagactcag aagacgacga agacgaagac gaagtcgagg aagaggaagg aacgacgccg ggaccaccgg caacaccagt accggcggga ccaacagtag tcgacgtttg taaaatagtg gatggaatac ttaaaggaaa tgatggaagc aatgaaataa gtggatgtaa tccaaaagag agttatcctg attgggattg caaaaaaaat attgacaaca gtcatagtgg agcatgtatg cctcctagaa gacaaaaatt atgcgtacgt gatttaacac aaggaggtga aataagaaaa ccagaagata tattaacaaa atttattaat tgtgcagcaa aagaaacaca ttttgcttgg cacaaatata aaaaggataa tgttaatgct gaaaatgaac taaaaagtgg gaaaattcca gaaggtttta gaaaacaaat gtattataca tttggtgatt ttagagatat attttttgga acagatattt catcgtgtcg atatattaaa gatacctcac aaacaataaa atctaaatta ggagatcaag caaccacgga aaaaggagat acacacattg atgataataa aaaactccaa gagtggtgga ccattcatgg tcctaaaata tgggaaggaa tgttatgcgc cttaacaaat ggtctcagtg aaagcgagaa agaaaatata ttgcaggatt actcatacaa caaactgaac aacgccgaaa aagatgattg ttgcctcgag aaatttgcct ccaaacctca atttttacgt tggtatgttg aatggagtga tgaattttgt cgagaacgga agaaattgga ggataaagta gaagatgttt gtattaaggc taaggattat gaagggtgta aaaacaataa gagtaataat agttgtgtta aagtatgtaa agaatatgaa aattatatta ccggaaaaaa aacacaatat gagagtcaag aaggcaaatt taatacagaa aaaagacaga agaaaccaga atataatagt tattctaaga aagacgcttc tgaatatctg aaagataaat gtttggatgg tacatgtgat tgtatgaaga aagtaaaaag cattgataat tattggaaaa atcctcataa aacgtatgac gataacaaac ttgaaactaa atgtgagtgt ccaccaacac caccaaaacc tgccagtaaa ccagacctcg cccgctccga cacgtaccaa ccaccagcac caccaaaacc acgcgctgga gaaggcctcg gtcgctcttt gccaaacgaa ccacgtgaca ccattgagga agacgacgac gacgacgacg aagacgaaga tgacgaaggc aaagtcgagg cggcgaagga agaccaggtg gacaggaagg gggaaacggc aaaggaggag gaaccaccgg catcacaaaa cgatgtaaat gtgtgcaaaa cggtggaaga agcacttaaa ggcaaattgg atgacgcttg taccctcaaa tatagtggaa acaactcacg tttaggttgg aagtgcatac caagtggaaa acctggtgac accggtagta gtggcgccat atgtgtgcca cccaggaggc gacgattata tgtcacacca ctaacgaagt gggcggaaac agtggcacaa ccacaggaga gtggtggtaa cacggagtca agtgacaaat tgcgtgaggc gttcatccaa tctgctgctg tggaaacttt ttttttgtgg cataaattta aaaaagaaaa agaaagagag gaaaaggaaa agaaagaagc aaatggtgaa ctgcccggct tctccagtag cgtcgacggt ggtgaacaaa acccccaaac cttgttacaa aatggtacca taccccccga tttcttgaga ttaatgtttt atactttagg agattataga gatatatgtg tgggtaagac accagatggt atagatacag tgagtggtaa agacacaatg gagaatataa aaaaagctat agaaagtgtt ttcaaaccta gtagtggcac cccatctctt agtgtcgaaa aaaccacacg agaatcctgg tgggaaaaaa acggtgaaca tatttggaaa gggatgattt gcgcattaac atacaaagaa aatggcgcag acggcacaaa caaaatagaa aaggatagtg atgtgtacaa aaaaattttc ggagaaaaca acaccgaccc tgacaaaaaa ctcaccgtca ctacagtcga caacaaaggc acattcgaaa gcaaatacaa atacgaaaaa gtacaactcg gagaagaaga agccagtggt cccaacgctc ccgcctcccc cacatccccc caagccaacg gaacccgcct ggccgagttt gtcacccgcc cagcgtactt ccgttacctt gaagaatggg gagaaacatt ttgtaaagag aggaagaaac ggttggagaa gattaaacag aagtgtaagg tagaagaaga tgaatacaaa tgtagtggtt ttggggaaga ttgtcaaaca aatcttaaaa atgatcctag tactattccg tctttagaat gtccagattg tgccaaacat tgtagttctt atagaaaatg gataggaaga aaaaaatacg aatttactga acaacaaaat gcatttacta aacaaaaaac ggatgctcat aataataatg gtttttgtgg aaaagaagga aagtgcaata cagctgccga ctttttagaa aggttaaaaa acggaccatg taaaaataat gagaacagaa cgaatgacat aaattttggt gatgtaaacg gtgaaacatt taaacatgca gaaaattgta atccatgttc tgaatttaaa ataaattgtg aaaatggtaa ttgtggtggt ggtacaaaag tgacatgccc tggaggaaag attactgcac aaaatatgaa agatatggaa aaaaatactg aaataaatat gcttgtgagt gatgacagta caaatggagt tgaaggtgat ttaaaggatt gtgaaaatga aggtatcttt gaaggtatta gaaaagatga atggatatgt ggtacggtat gtggttataa tgtgtgtaaa ccggcaaaag tcaatgggca aaaagatggt gaaaaacaca ttataacaat tagagctttg gttacacatt gggtacataa ttttttagaa gattataata gaattaaaca tagaatttca cattgtatgg aaaagggtga accatccaat tgtataaatg gatgtaataa aaaatgtaat tgtgtaaaaa aatggataag tacgaaaaag caagaatggc aacaaataaa aaaacaatac cttgaacaat ataaaaatca aggtgagtct taccctacga aaactgtatt ggaggaattt aaggaacggc ctgtacttaa caaagctata aaaccttgtg gtggtttaga gaagtttcaa aattcaactg attgtactgt cgcagggagc tcagaaagtg gtaaagatgg tacacaaaag gatatcgtag aatgtttgct tcataagctt gaaacaaaag caaaaaattg tccaggcaaa cctagtgccg aagaacaaaa accttgtgat gtatccaacc ccctcgttga agatgacgac gaacacttcc ttgaagagga agaccaaaac ccagaagaaa atacagtggg aaaacaacaa ccatcatttt gtccgccagt ggaagataaa aaaaaagagg aagaagaaga aacttgtaca ccggcatcac cagtaccaga aaaaccggta cctcaggtgc catgttggcg accatttaaa ccacctaata tgttcccgaa acggataaaa aaaaaaaatg cgtgcgaaat agcggaagaa atacttaagg ataatgatgg aaagaaacaa gtaggtcaat gtcatccaaa aaataatgat aagaattatc ttcaatggaa ttgtgatgaa aacaaaatta aaaccacaga ggttggagcc tgtatgcctc ctagaagaca aaaactatgt ttatattatt taacacaatt gaattacaat gacaatgcag ataaattaag agaagcattt attaaatctg cagcagcaga aacatttctc tcatggcact attttaagac taagaatggt gataatgcac agacacagtt aaaagcagga aaaatccctg ccgaattttt gagatcgatg ttcaatacat atggtgatta tagagatttg ttcttggaaa aagatataag tagcgaagtt catacaataa aagataatat aaataaggtt ttcagaaata gtgtcagacg tagaggcgaa acaagtgata caaaacgcca aaagtggtgg aatgaacatg gtccagagat atggaaaggg atgttatgtg ctttaagtta tgatactgaa gaaaaaaatg ttaatacgca aattcgacaa agacttacga aatactacga ctacaaaaat gtggcattca cgtacaaaag tggtactcct ttggagaaat tcgcttctag acctcaattt ctacgatggt ttactgaatg gggagaagat ttctgcacac aacgaaagaa acagtttgag attttacagg gaaagtgcgt ggaatgtact gttagtgata gtggtactag tgataaaact aaaacatgtg atgataagaa aaattgtgac acgtgtaaaa cagcatgtac aacatatcaa aattggcttc aaaagtggaa aactgattat gcaaaacaaa ggaacaaata tgctgaagat aaaaaaaagg atctatataa aagtattgat gacgtaaaaa attcaccaaa tgcctttgaa tatttataca aacaatcaaa acactttata tgtgaaaatg gtgattgtaa ttgtatggaa aattcgtcca aacaacaaaa acagtcatct actgatggtg ataaaatgcc cgaatcgttg gacgaaaagc cggaaaaggt caaagacaag tgtaattgtg ttctacatga atgtagtgga ttgagtgtaa ctgatagcgg tattcctgat ggtagtgctt ttggtggagg attacctcca ggaaaatgta aaggattaga aggaggtccc cagaaaaaaa tcgaaccccc gacaagtgat tatataaatg atatattgaa aagtactatc cctgttggaa ttgctttggc attaggttca atagcttttc tgttcatgaa gaagaaaccc aaaacacctg tggacctgtt acgtgtactt gatattcata aaggcgatta tggaatgcct acgttggaat ccaaaaatag gtacatacca tatagaagtg gtacatataa aggcaaaaca tacatttata tggaaggaga ttcagatgaa gataaatatg catttatgtc tgatactact gatgtaactt cctccgaaag tgagtatgaa gaattggata ttaatgatat atatgtacct ggtagtccta aatataaaac attgatagaa gtggtactag aaccatcgaa aagagatata ccaagtggtg atataccaaa taataataca cctatgaata aatttacaga tgaggaatgg aatcaattga aacacgattt tatatctcaa tatttaccaa atacagaacc taatacttta tattttgata aacctgaaga aaaacctttt attacttcta ttcatgatag aaatttatat actggggaag aatatagtta taatattaat atgagtacta atactatgga tgatccaaaa tatgtatcaa ataatgtata ttctggtatt gacctaatta atgattcatt aaatagtggt aatcaaccta ttgatatata tgatgaagtg ttgaaacgaa aagaaaatga attattcggg acagaacatc acccaaaacg tacaacaact aaccatttcg ccacacctac acgtgacgac cccatccaca accaactgga actattccat aaatggttag atcgacatag agatatgtgc gagaagtgga ataataaaga ggaagtatta gataaattaa aagaagagtg ggaaaatgag acacatagtg gtaacactca ccctagtgat agtaacaaaa cgttgaatac gaatgtttct attcagatag atatggatca tgaaaaacga atgaaggaat ttactaatat ggatacatat ccggagaatt ctactatgga tagtatattg gaggatctgg aaaaatataa agaaccttat tatgatgtgc aagatgatat ttattatgat gtaaatgatc atgatacatc aactgtgggt agtaataata tggatgttcc tagtaaagta caaattgaaa tggatgtaaa taccaaattg gttaaagaga aatatcctat atcagatata tggaatatat aa
Details for PlfaA.18519.a.B21.GE37683
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 57kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 71
Validated AA Sequence
MKAEKINSKD NLLLEVCLAA QYEGESLVEK HKEFKKTHND SNICTILARS FADIGDIIRG KDLYLGNKKE KEKLENNLKK IFKEIYKDVM KTSGSNGKTN GEIEARYGSD KDPNYYELRE DWWDANRREV WKAMTCNADN DSQYFRQTCG SREKGSATKG KCRCVIGDVP TYFDYVPQYL RWFQEWAEDF CRIKKIKLEL AKKACRDESS KLYCSHNGYD CTKRIEKGKS CSRDSKCTEC STKCIPYEYW LEKQQNEFNM QKDKYDKEIR TKTSNNGISN SNINNKYYKE FYNELNKKYE SVENFLTLLN NGMYCKKHVD GESTINFNKT DDKGAFYRSD YCQPCPDCVV V
Validated NT Sequence
atcttactca cttaaacaac tacacaatcg ggacagggtt ggcaataatc tgagcgataa aacgcacctt tatcatcagt cttattaaaa ttaatagtac tttctccatc cacatgtttt ttacaataca ttccattatt tagtaaggtt aaaaaatttt caacgctttc atatttttta ttaagttcgt tataaaattc cttataatat ttgttattaa tattactatt agatataccg ttgttggatg tctttgttct tatttcctta tcatatttat ccttttgcat gttaaattca ttttgttgtt tctctaacca atattcataa ggaatgcatt tagtcgaaca ttcagtacac ttactatccc tagagcaact ttttcctttt tcaatacgtt tcgtacaatc atatccatta tgactacaat ataattttga tgattcatca cgacacgctt tcttggccaa ttctaattta atttttttta ttctacaaaa gtcttcggcc cattcctgga accagcgaag atactgcggc acgtagtcaa aatatgtggg aacatctcca atcacacatc ggcatttacc tttagtcgca cttcccttct ctcttgaacc acatgtttgt cgaaaatatt gagaatcatt atcagcattg catgtcattg ctttccatac ctctcttcta ttagcatccc accaatcttc tctcaattca taataatttg gatctttatc acttccgtag cgagcttcta tctccccatt cgtcttccca ttactcccgc tcgtcttcat cacgtcctta tatatttcct tgaaaatttt ttttaaatta ttttctaatt tctctttttc tttcttatta ccaagataca gatcttttcc tctaataata tctcctatat ctgcaaaact tcgtgccaat atagtacata tattggaatc gttatgtgtt tttttaaatt ctttatgttt ttctactaat gattctcctt catattgtgc tgcaagacac acttctaaca acaaattatc tttagaatta attttttcag ctttcatgtg cgataaatgt tggtcacata gatgtaatcg tctgaacggc gcgcaggctc caatgtcttt accattacta tcatcattac catgtatttt actattagtg cattgaccac catatttatc agaaaagcga atgtcccatc tatctttaca aggatgctcc ctaccataac catccgtaac attagtatga tatatatgat taagtttaca taattcggct tttccacgct cgatgttctt acgatcttta aatgatgctt ctgacaaacg tccttgcaaa gcgcttctat aatctacagc ttttttttcc actttttctt tgtacacttt ttcccctatg ctatccaata aatgtttggc atcactgtag tcagtcacag ccgccttggg
Expected Protein Sequence
MAHHHHHHMG PKAAVTDYSD AKHLLDSIGE KVYKEKVEKK AVDYRSALQG RLSEASFKDR KNIERGKAEL CKLNHIYHTN VTDGYGREHP CKDRWDIRFS DKYGGQCTNS KIHGNDDSNG KDIGACAPFR RLHLCDQHLS HMKAEKINSK DNLLLEVCLA AQYEGESLVE KHKEFKKTHN DSNICTILAR SFADIGDIIR GKDLYLGNKK EKEKLENNLK KIFKEIYKDV MKTSGSNGKT NGEIEARYGS DKDPNYYELR EDWWDANRRE VWKAMTCNAD NDSQYFRQTC GSREKGSATK GKCRCVIGDV PTYFDYVPQY LRWFQEWAED FCRIKKIKLE LAKKACRDES SKLYCSHNGY DCTKRIEKGK SCSRDSKCTE CSTKCIPYEY WLEKQQNEFN MQKDKYDKEI RTKTSNNGIS NSNINNKYYK EFYNELNKKY ESVENFLTLL NNGMYCKKHV DGESTINFNK TDDKGAFYRS DYCQPCPDCV VV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggc ccaaggcggc tgtgactgac tacagtgatg ccaaacattt attggatagc ataggggaaa aagtgtacaa agaaaaagtg gaaaaaaaag ctgtagatta tagaagcgct ttgcaaggac gtttgtcaga agcatcattt aaagatcgta agaacatcga gcgtggaaaa gccgaattat gtaaacttaa tcatatatat catactaatg ttacggatgg ttatggtagg gagcatcctt gtaaagatag atgggacatt cgcttttctg ataaatatgg tggtcaatgc actaatagta aaatacatgg taatgatgat agtaatggta aagacattgg agcctgcgcg ccgttcagac gattacatct atgtgaccaa catttatcgc acatgaaagc tgaaaaaatt aattctaaag ataatttgtt gttagaagtg tgtcttgcag cacaatatga aggagaatca ttagtagaaa aacataaaga atttaaaaaa acacataacg attccaatat atgtactata ttggcacgaa gttttgcaga tataggagat attattagag gaaaagatct gtatcttggt aataagaaag aaaaagagaa attagaaaat aatttaaaaa aaattttcaa ggaaatatat aaggacgtga tgaagacgag cgggagtaat gggaagacga atggggagat agaagctcgc tacggaagtg ataaagatcc aaattattat gaattgagag aagattggtg ggatgctaat agaagagagg tatggaaagc aatgacatgc aatgctgata atgattctca atattttcga caaacatgtg gttcaagaga gaagggaagt gcgactaaag gtaaatgccg atgtgtgatt ggagatgttc ccacatattt tgactacgtg ccgcagtatc ttcgctggtt ccaggaatgg gccgaagact tttgtagaat aaaaaaaatt aaattagaat tggccaagaa agcgtgtcgt gatgaatcat caaaattata ttgtagtcat aatggatatg attgtacgaa acgtattgaa aaaggaaaaa gttgctctag ggatagtaag tgtactgaat gttcgactaa atgcattcct tatgaatatt ggttagagaa acaacaaaat gaatttaaca tgcaaaagga taaatatgat aaggaaataa gaacaaagac atccaacaac ggtatatcta atagtaatat taataacaaa tattataagg aattttataa cgaacttaat aaaaaatatg aaagcgttga aaatttttta accttactaa ataatggaat gtattgtaaa aaacatgtgg atggagaaag tactattaat tttaataaga ctgatgataa aggtgcgttt tatcgctcag attattgcca accctgtccc gattgtgtag ttgtttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PlfaA.18519.a.B21.PW37469
PURIFICATION DATe: 4/1/2014
CONCENTRATION: 14.6mg/ml
OBSERVED MW: 58kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 71
Protocol Notes
notes unavailable
Validated AA Sequence
MKAEKINSKD NLLLEVCLAA QYEGESLVEK HKEFKKTHND SNICTILARS FADIGDIIRG KDLYLGNKKE KEKLENNLKK IFKEIYKDVM KTSGSNGKTN GEIEARYGSD KDPNYYELRE DWWDANRREV WKAMTCNADN DSQYFRQTCG SREKGSATKG KCRCVIGDVP TYFDYVPQYL RWFQEWAEDF CRIKKIKLEL AKKACRDESS KLYCSHNGYD CTKRIEKGKS CSRDSKCTEC STKCIPYEYW LEKQQNEFNM QKDKYDKEIR TKTSNNGISN SNINNKYYKE FYNELNKKYE SVENFLTLLN NGMYCKKHVD GESTINFNKT DDKGAFYRSD YCQPCPDCVV V
Validated NT Sequence
atcttactca cttaaacaac tacacaatcg ggacagggtt ggcaataatc tgagcgataa aacgcacctt tatcatcagt cttattaaaa ttaatagtac tttctccatc cacatgtttt ttacaataca ttccattatt tagtaaggtt aaaaaatttt caacgctttc atatttttta ttaagttcgt tataaaattc cttataatat ttgttattaa tattactatt agatataccg ttgttggatg tctttgttct tatttcctta tcatatttat ccttttgcat gttaaattca ttttgttgtt tctctaacca atattcataa ggaatgcatt tagtcgaaca ttcagtacac ttactatccc tagagcaact ttttcctttt tcaatacgtt tcgtacaatc atatccatta tgactacaat ataattttga tgattcatca cgacacgctt tcttggccaa ttctaattta atttttttta ttctacaaaa gtcttcggcc cattcctgga accagcgaag atactgcggc acgtagtcaa aatatgtggg aacatctcca atcacacatc ggcatttacc tttagtcgca cttcccttct ctcttgaacc acatgtttgt cgaaaatatt gagaatcatt atcagcattg catgtcattg ctttccatac ctctcttcta ttagcatccc accaatcttc tctcaattca taataatttg gatctttatc acttccgtag cgagcttcta tctccccatt cgtcttccca ttactcccgc tcgtcttcat cacgtcctta tatatttcct tgaaaatttt ttttaaatta ttttctaatt tctctttttc tttcttatta ccaagataca gatcttttcc tctaataata tctcctatat ctgcaaaact tcgtgccaat atagtacata tattggaatc gttatgtgtt tttttaaatt ctttatgttt ttctactaat gattctcctt catattgtgc tgcaagacac acttctaaca acaaattatc tttagaatta attttttcag ctttcatgtg cgataaatgt tggtcacata gatgtaatcg tctgaacggc gcgcaggctc caatgtcttt accattacta tcatcattac catgtatttt actattagtg cattgaccac catatttatc agaaaagcga atgtcccatc tatctttaca aggatgctcc ctaccataac catccgtaac attagtatga tatatatgat taagtttaca taattcggct tttccacgct cgatgttctt acgatcttta aatgatgctt ctgacaaacg tccttgcaaa gcgcttctat aatctacagc ttttttttcc actttttctt tgtacacttt ttcccctatg ctatccaata aatgtttggc atcactgtag tcagtcacag ccgccttggg
Expressed Protein Sequence
MAHHHHHHMG PKAAVTDYSD AKHLLDSIGE KVYKEKVEKK AVDYRSALQG RLSEASFKDR KNIERGKAEL CKLNHIYHTN VTDGYGREHP CKDRWDIRFS DKYGGQCTNS KIHGNDDSNG KDIGACAPFR RLHLCDQHLS HMKAEKINSK DNLLLEVCLA AQYEGESLVE KHKEFKKTHN DSNICTILAR SFADIGDIIR GKDLYLGNKK EKEKLENNLK KIFKEIYKDV MKTSGSNGKT NGEIEARYGS DKDPNYYELR EDWWDANRRE VWKAMTCNAD NDSQYFRQTC GSREKGSATK GKCRCVIGDV PTYFDYVPQY LRWFQEWAED FCRIKKIKLE LAKKACRDES SKLYCSHNGY DCTKRIEKGK SCSRDSKCTE CSTKCIPYEY WLEKQQNEFN MQKDKYDKEI RTKTSNNGIS NSNINNKYYK EFYNELNKKY ESVENFLTLL NNGMYCKKHV DGESTINFNK TDDKGAFYRS DYCQPCPDCV VV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGGC CCAAGGCGGC TGTGACTGAC TACAGTGATG CCAAACATTT ATTGGATAGC ATAGGGGAAA AAGTGTACAA AGAAAAAGTG GAAAAAAAAG CTGTAGATTA TAGAAGCGCT TTGCAAGGAC GTTTGTCAGA AGCATCATTT AAAGATCGTA AGAACATCGA GCGTGGAAAA GCCGAATTAT GTAAACTTAA TCATATATAT CATACTAATG TTACGGATGG TTATGGTAGG GAGCATCCTT GTAAAGATAG ATGGGACATT CGCTTTTCTG ATAAATATGG TGGTCAATGC ACTAATAGTA AAATACATGG TAATGATGAT AGTAATGGTA AAGACATTGG AGCCTGCGCG CCGTTCAGAC GATTACATCT ATGTGACCAA CATTTATCGC ACATGAAAGC TGAAAAAATT AATTCTAAAG ATAATTTGTT GTTAGAAGTG TGTCTTGCAG CACAATATGA AGGAGAATCA TTAGTAGAAA AACATAAAGA ATTTAAAAAA ACACATAACG ATTCCAATAT ATGTACTATA TTGGCACGAA GTTTTGCAGA TATAGGAGAT ATTATTAGAG GAAAAGATCT GTATCTTGGT AATAAGAAAG AAAAAGAGAA ATTAGAAAAT AATTTAAAAA AAATTTTCAA GGAAATATAT AAGGACGTGA TGAAGACGAG CGGGAGTAAT GGGAAGACGA ATGGGGAGAT AGAAGCTCGC TACGGAAGTG ATAAAGATCC AAATTATTAT GAATTGAGAG AAGATTGGTG GGATGCTAAT AGAAGAGAGG TATGGAAAGC AATGACATGC AATGCTGATA ATGATTCTCA ATATTTTCGA CAAACATGTG GTTCAAGAGA GAAGGGAAGT GCGACTAAAG GTAAATGCCG ATGTGTGATT GGAGATGTTC CCACATATTT TGACTACGTG CCGCAGTATC TTCGCTGGTT CCAGGAATGG GCCGAAGACT TTTGTAGAAT AAAAAAAATT AAATTAGAAT TGGCCAAGAA AGCGTGTCGT GATGAATCAT CAAAATTATA TTGTAGTCAT AATGGATATG ATTGTACGAA ACGTATTGAA AAAGGAAAAA GTTGCTCTAG GGATAGTAAG TGTACTGAAT GTTCGACTAA ATGCATTCCT TATGAATATT GGTTAGAGAA ACAACAAAAT GAATTTAACA TGCAAAAGGA TAAATATGAT AAGGAAATAA GAACAAAGAC ATCCAACAAC GGTATATCTA ATAGTAATAT TAATAACAAA TATTATAAGG AATTTTATAA CGAACTTAAT AAAAAATATG AAAGCGTTGA AAATTTTTTA ACCTTACTAA ATAATGGAAT GTATTGTAAA AAACATGTGG ATGGAGAAAG TACTATTAAT TTTAATAAGA CTGATGATAA AGGTGCGTTT TATCGCTCAG ATTATTGCCA ACCCTGTCCC GATTGTGTAG TTGTTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT