PlfaA.18484.a

Chitinase (EC 3.2.1.14)

CENTER ID: PlfaA.18484.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PlfaA.18484.a.B2.GE35258 Crowther chunk 28 378
PlfaA.18484.a.B3.GE36463 Crowther superfamily domain 1 30 234
PlfaA.18484.a.B4.GE36464 Crowther homologous domain 1 30 378
PlfaA.18484.a.B5.GE36465 Crowther superfamily domain 2 52 234
PlfaA.18484.a.B6.GE36466 Crowther homologous domain 2 52 378
PlfaA.18484.a.B7.GE36467 Gilson SMART domain 30 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1252200
RefSeq: XP_001350904.1
UniProt: Q8I4R4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNFTVKYSFL VICLLCCLLS TYVSVIEGHR ARPGESRKNP REIIKTFKES GKGIIQGYYP SWVSYNHNLK DLNPNLNVVH MSFAKMDLSY DSIESIVGSP LLFKSLIGLE YIGLNEYFND AMNLRKARPD IIMLLSLGGE TYHPSSFDSA LNAVEKIANL VDELGFDGID VDYEPNGSFD GLNDKEKADF FVQYVTKLRE YMCDDKLISI SQSSNGALSC IGFNDPKKIC MDDEAPYNSK YFNKPDVKKE LLRAAQMASA GGAIYLMNNL KDMIDMVFVQ TFNYTNSTDS TVMKELYDSY AYYGKKYDYV IIMGFTLMFP STPFNPNDKM LVKSIGDFVK TENKLNKRAD GFGLWSLSSD NAAHNEQLAI EYFVESLH
NT Sequence
atgaatttta ctgtgaaata ttcattttta gttatttgtt tattatgctg tttattaagc acttatgtaa gtgtcattga gggacatcga gcacgaccag gtgaatcaag aaaaaacccg agagaaatta taaaaacatt taaagagagt gggaaaggaa ttattcaagg ttattatcca tcatgggttt cttataatca taacttgaaa gatttaaatc ctaatttaaa tgttgtacat atgtcttttg ctaagatgga tttatcctat gacagtatag agagtattgt tggttctcct ttacttttca agtcattaat aggtttagaa tatatagggt taaacgaata ttttaatgat gccatgaatt taagaaaagc tcgtccagat attattatgc ttttatctct tggaggagaa acatatcatc caagttcatt tgattctgct ttaaatgctg ttgagaaaat tgcaaattta gttgatgaat taggatttga tggaatcgat gttgattatg agcctaatgg atcttttgat ggtttgaacg ataaagagaa agctgatttt ttcgtacaat atgtaacaaa attgagggaa tatatgtgtg atgataaatt aatttctata tctcaatcat ctaacggagc tttaagttgt ataggtttta atgatcctaa gaaaatatgt atggatgatg aagcaccata taattcaaaa tatttcaata aacctgatgt aaaaaaggag ctattaagag cagcgcaaat ggcttcagct ggaggagcaa tatatttaat gaataattta aaggatatga ttgatatggt gtttgtacaa acctttaatt atacaaattc tacagattca acagttatga aagaattata tgattcttat gcttattatg gaaaaaaata tgattatgtt attataatgg gatttacatt aatgtttcct tcaacccctt ttaatccgaa tgataaaatg cttgtaaaat ccattggtga ttttgtaaag actgaaaata aattaaataa aagagcagac gggtttggat tatggtcttt atcttctgat aatgctgcac ataatgaaca attagcaatt gaatatttcg tagaatcttt acattaa
Details for PlfaA.18484.a.B2.GE35258
HARVESTED ON: 5/1/2012
SEQUENCED ON: 8/1/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 97
PERCENT COVERAGE: 81
Validated AA Sequence
MAHHHHHHMG HRARPGESRK NPREIIKTFK ESGKGIIQGY YPSWVSYNHN LKDLNPNLNV VHMSFAKMDL SYDSIESIVG SPLLFKSLIG LEYIGLNEYF NDAMNLRKAR PDIIMLLSLG GETYHPSSFD SALNAVEKIA NLVDELGFDG IDVDYEPNGS FDGLNDKEKA DFFVQYVTKL REYMCDDKLI SISQSSNGAL SCIGFNDPKK ICMDDEAPYN SKYFNKPDVK KELLRAAQMA SAGGAIYLMN NLKDMIDMVF VQTFNYTNST DSTVMKELYD SYAYYGKNMI MLL
Validated NT Sequence
atggctcacc accaccacca ccatatggga catcgagcac gaccaggtga atcaagaaaa aacccgagag aaattataaa aacatttaaa gagagtggga aaggaattat tcaaggttat tatccatcat gggtttctta taatcataac ttgaaagatt taaatcctaa tttaaatgtt gtacatatgt cttttgctaa gatggattta tcctatgaca gtatagagag tattgttggt tctcctttac ttttcaagtc attaataggt ttagaatata tagggttaaa cgaatatttt aatgatgcca tgaatttaag aaaagctcgt ccagatatta ttatgctttt atctcttgga ggagaaacat atcatccaag ttcatttgat tctgctttaa atgctgttga gaaaattgca aatttagttg atgaattagg atttgatgga atcgatgttg attatgagcc taatggatct tttgatggtt tgaacgataa agagaaagct gattttttcg tacaatatgt aacaaaattg agggaatata tgtgtgatga taaattaatt tctatatctc aatcatctaa cggagcttta agttgtatag gttttaatga tcctaagaaa atatgtatgg atgatgaagc accatataat tcaaaatatt tcaataaacc tgatgtaaaa aaggagctat taagagcagc gcaaatggct tcagctggag gagcaatata tttaatgaat aatttaaagg atatgattga tatggtgttt gtacaaacct ttaattatac aaattctaca gattcnacag ttatgaaaga attatatgat tcttatgctt attatggaaa aaatatgatt atgttattat aatgggattt acnttaatgn ttccttcaac cccttttaat ccnaatgata aaatgcttgt aaaatccatt ggngattttg naaanactga aaataaatta aataaa
Expected Protein Sequence
MAHHHHHHMG HRARPGESRK NPREIIKTFK ESGKGIIQGY YPSWVSYNHN LKDLNPNLNV VHMSFAKMDL SYDSIESIVG SPLLFKSLIG LEYIGLNEYF NDAMNLRKAR PDIIMLLSLG GETYHPSSFD SALNAVEKIA NLVDELGFDG IDVDYEPNGS FDGLNDKEKA DFFVQYVTKL REYMCDDKLI SISQSSNGAL SCIGFNDPKK ICMDDEAPYN SKYFNKPDVK KELLRAAQMA SAGGAIYLMN NLKDMIDMVF VQTFNYTNST DSTVMKELYD SYAYYGKKYD YVIIMGFTLM FPSTPFNPND KMLVKSIGDF VKTENKLNKR ADGFGLWSLS SDNAAHNEQL AIEYFVESLH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggac atcgagcacg accaggtgaa tcaagaaaaa acccgagaga aattataaaa acatttaaag agagtgggaa aggaattatt caaggttatt atccatcatg ggtttcttat aatcataact tgaaagattt aaatcctaat ttaaatgttg tacatatgtc ttttgctaag atggatttat cctatgacag tatagagagt attgttggtt ctcctttact tttcaagtca ttaataggtt tagaatatat agggttaaac gaatatttta atgatgccat gaatttaaga aaagctcgtc cagatattat tatgctttta tctcttggag gagaaacata tcatccaagt tcatttgatt ctgctttaaa tgctgttgag aaaattgcaa atttagttga tgaattagga tttgatggaa tcgatgttga ttatgagcct aatggatctt ttgatggttt gaacgataaa gagaaagctg attttttcgt acaatatgta acaaaattga gggaatatat gtgtgatgat aaattaattt ctatatctca atcatctaac ggagctttaa gttgtatagg ttttaatgat cctaagaaaa tatgtatgga tgatgaagca ccatataatt caaaatattt caataaacct gatgtaaaaa aggagctatt aagagcagcg caaatggctt cagctggagg agcaatatat ttaatgaata atttaaagga tatgattgat atggtgtttg tacaaacctt taattataca aattctacag attcaacagt tatgaaagaa ttatatgatt cttatgctta ttatggaaaa aaatatgatt atgttattat aatgggattt acattaatgt ttccttcaac cccttttaat ccgaatgata aaatgcttgt aaaatccatt ggtgattttg taaagactga aaataaatta aataaaagag cagacgggtt tggattatgg tctttatctt ctgataatgc tgcacataat gaacaattag caattgaata tttcgtagaa tctttacatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for PlfaA.18484.a.B3.GE36463
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR ARPGESRKNP REIIKTFKES GKGIIQGYYP SWVSYNHNLK DLNPNLNVVH MSFAKMDLSY DSIESIVGSP LLFKSLIGLE YIGLNEYFND AMNLRKARPD IIMLLSLGGE TYHPSSFDSA LNAVEKIANL VDELGFDGID VDYEPNGSFD GLNDKEKADF FVQYVTKLRE YMCDDKLISI SQSSNGALSC IGFNDPKKIC MDDE
Validated NT Sequence
ttgtnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgcgagca cgaccaggtg aatcaagaaa aaacccgaga gaaattataa aaacatttaa agagagtggg aaaggaatta ttcaaggtta ttatccatca tgggtttctt ataatcataa cttgaaagat ttaaatccta atttaaatgt tgtacatatg tcttttgcta agatggattt atcctatgac agtatagaga gtattgttgg ttctccttta cttttcaagt cattaatagg tttagaatat atagggttaa acgaatattt taatgatgcc atgaatttaa gaaaagctcg tccagatatt attatgcttt tatctcttgg aggagaaaca tatcatccaa gttcatttga ttctgcttta aatgctgttg agaaaattgc aaatttagtt gatgaattag gatttgatgg aatcgatgtt gattatgagc ctaatggatc ttttgatggt ttgaacgata aagagaaagc tgattttttc gtacaatatg taacaaaatt gagggaatat atgtgtgatg ataaattaat ttctatatct caatcatcta acggagcttt aagttgtata ggttttaatg atcctaagaa aatatgtatg gatgatgaat aagtgagtaa gataggatcc ggctgctaac nnnccccnaa aagaannnnn gnnnnnnnnn nnnnnnnnnn cttnnnnnnn nnnnnnnnnt nnnnnncnat ncnnnnnnnn nnnnncc
Expected Protein Sequence
MAHHHHHHMR ARPGESRKNP REIIKTFKES GKGIIQGYYP SWVSYNHNLK DLNPNLNVVH MSFAKMDLSY DSIESIVGSP LLFKSLIGLE YIGLNEYFND AMNLRKARPD IIMLLSLGGE TYHPSSFDSA LNAVEKIANL VDELGFDGID VDYEPNGSFD GLNDKEKADF FVQYVTKLRE YMCDDKLISI SQSSNGALSC IGFNDPKKIC MDDE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgag cacgaccagg tgaatcaaga aaaaacccga gagaaattat aaaaacattt aaagagagtg ggaaaggaat tattcaaggt tattatccat catgggtttc ttataatcat aacttgaaag atttaaatcc taatttaaat gttgtacata tgtcttttgc taagatggat ttatcctatg acagtataga gagtattgtt ggttctcctt tacttttcaa gtcattaata ggtttagaat atatagggtt aaacgaatat tttaatgatg ccatgaattt aagaaaagct cgtccagata ttattatgct tttatctctt ggaggagaaa catatcatcc aagttcattt gattctgctt taaatgctgt tgagaaaatt gcaaatttag ttgatgaatt aggatttgat ggaatcgatg ttgattatga gcctaatgga tcttttgatg gtttgaacga taaagagaaa gctgattttt tcgtacaata tgtaacaaaa ttgagggaat atatgtgtga tgataaatta atttctatat ctcaatcatc taacggagct ttaagttgta taggttttaa tgatcctaag aaaatatgta tggatgatga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for PlfaA.18484.a.B4.GE36464
HARVESTED ON: 11/21/2012
SEQUENCED ON: 1/14/2013
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 81
Validated AA Sequence
MAHHHHHHMR ARPGESRKNP REIIKTFKES GKGIIQGYYP SWVSYNHNLK DLNPNLNVVH MSFAKMDLSY DSIESIVGSP LLFKSLIGLE YIGLNEYFND AMNLRKARPD IIMLLSLGGE TYHPSSFDSA LNAVEKIANL VDELGFDGID VDYEPNGSFD GLNDKEKADF FVQYVTKLRE YMCDDKLISI SQSSNGALSC IGFNDPKKIC MDDEAPYNSK YFNKPDVKKE LLRAAQMASA GGAIYLMNNL KDMIDMVFVQ TFNYTNSTDS TVMKELYDSY AYYGKNMIML L
Validated NT Sequence
anattttgtn nnntttaaga aggagatata ccatggctca ccaccaccac caccatatgc gagcacgacc aggtgaatca agaaaaaacc cgagagaaat tataaaaaca tttaaagaga gtgggaaagg aattattcaa ggttattatc catcatgggt ttcttataat cataacttga aagatttaaa tcctaattta aatgttgtac atatgtcttt tgctaagatg gatttatcct atgacagtat agagagtatt gttggttctc ctttactttt caagtcatta ataggtttag aatatatagg gttaaacgaa tattttaatg atgccatgaa tttaagaaaa gctcgtccag atattattat gcttttatct cttggaggag aaacatatca tccaagttca tttgattctg ctttaaatgc tgttgagaaa attgcaaatt tagttgatga attaggattt gatggaatcg atgttgatta tgagcctaat ggatcttttg atggtttgaa cgataaagag aaagctgatt ttttcgtaca atatgtaaca aaattgaggg aatatatgtg tgatgataaa ttaatttcta tatctcaatc atctaacgga gctttaagtt gtataggttt taatgatcct aagaaaatat gtatggatga tgaagcacca tataattcaa aatatttcaa taaacctgat gtaaaaaagg agctattaag agcagcgcaa atggcttcag ctggaggagc aatatattta atgaataatt taaaggatat gattgatatg gtgtttgtac aaacctttaa ttatacaaat tctacagatt caacagttat gaaagaatta tatgattctt atgcttatta tggaaaaaat atgattatgt tattataatg ggatttacat taatgtttcc ttcaacccct tttaatccna atgataaaat gcttgtaaat ccattggtga tttntaaana ctgaaataaa ttaaataaan agcananggn ttggatnatg gncnttnnnn ncngannngc tgcncntnan nacnnnnnan tgaannnttc nnanannnnn nnnnnnnnnt annnnnnnnt t
Expected Protein Sequence
MAHHHHHHMR ARPGESRKNP REIIKTFKES GKGIIQGYYP SWVSYNHNLK DLNPNLNVVH MSFAKMDLSY DSIESIVGSP LLFKSLIGLE YIGLNEYFND AMNLRKARPD IIMLLSLGGE TYHPSSFDSA LNAVEKIANL VDELGFDGID VDYEPNGSFD GLNDKEKADF FVQYVTKLRE YMCDDKLISI SQSSNGALSC IGFNDPKKIC MDDEAPYNSK YFNKPDVKKE LLRAAQMASA GGAIYLMNNL KDMIDMVFVQ TFNYTNSTDS TVMKELYDSY AYYGKKYDYV IIMGFTLMFP STPFNPNDKM LVKSIGDFVK TENKLNKRAD GFGLWSLSSD NAAHNEQLAI EYFVESLH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgag cacgaccagg tgaatcaaga aaaaacccga gagaaattat aaaaacattt aaagagagtg ggaaaggaat tattcaaggt tattatccat catgggtttc ttataatcat aacttgaaag atttaaatcc taatttaaat gttgtacata tgtcttttgc taagatggat ttatcctatg acagtataga gagtattgtt ggttctcctt tacttttcaa gtcattaata ggtttagaat atatagggtt aaacgaatat tttaatgatg ccatgaattt aagaaaagct cgtccagata ttattatgct tttatctctt ggaggagaaa catatcatcc aagttcattt gattctgctt taaatgctgt tgagaaaatt gcaaatttag ttgatgaatt aggatttgat ggaatcgatg ttgattatga gcctaatgga tcttttgatg gtttgaacga taaagagaaa gctgattttt tcgtacaata tgtaacaaaa ttgagggaat atatgtgtga tgataaatta atttctatat ctcaatcatc taacggagct ttaagttgta taggttttaa tgatcctaag aaaatatgta tggatgatga agcaccatat aattcaaaat atttcaataa acctgatgta aaaaaggagc tattaagagc agcgcaaatg gcttcagctg gaggagcaat atatttaatg aataatttaa aggatatgat tgatatggtg tttgtacaaa cctttaatta tacaaattct acagattcaa cagttatgaa agaattatat gattcttatg cttattatgg aaaaaaatat gattatgtta ttataatggg atttacatta atgtttcctt caaccccttt taatccgaat gataaaatgc ttgtaaaatc cattggtgat tttgtaaaga ctgaaaataa attaaataaa agagcagacg ggtttggatt atggtcttta tcttctgata atgctgcaca taatgaacaa ttagcaattg aatatttcgt agaatcttta cattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PlfaA.18484.a.B5.GE36465
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK GIIQGYYPSW VSYNHNLKDL NPNLNVVHMS FAKMDLSYDS IESIVGSPLL FKSLIGLEYI GLNEYFNDAM NLRKARPDII MLLSLGGETY HPSSFDSALN AVEKIANLVD ELGFDGIDVD YEPNGSFDGL NDKEKADFFV QYVTKLREYM CDDKLISISQ SSNGALSCIG FNDPKKICMD DE
Validated NT Sequence
ttnnnnnnnt taagaaggag atataccatg gctcaccacc accaccacca tatgaaagga attattcaag gttattatcc atcatgggtt tcttataatc ataacttgaa agatttaaat cctaatttaa atgttgtaca tatgtctttt gctaagatgg atttatccta tgacagtata gagagtattg ttggttctcc tttacttttc aagtcattaa taggtttaga atatataggg ttaaacgaat attttaatga tgccatgaat ttaagaaaag ctcgtccaga tattattatg cttttatctc ttggaggaga aacatatcat ccaagttcat ttgattctgc tttaaatgct gttgagaaaa ttgcaaattt agttgatgaa ttaggatttg atggaatcga tgttgattat gagcctaatg gatcttttga tggtttgaac gataaagaga aagctgattt tttcgtacaa tatgtaacaa aattgaggga atatatgtgt gatgataaat taatttctat atctcaatca tctaacggag ctttaagttg tataggtttt aatgatccta agaaaatatg tatggatgat gaataagtga gtaagatagg atccggnncc cccnaanaga nannnnnnnn aaaaaaaann nnnnnnnnnn nnnnncntnt ttnacgcnnc nntnnnnngn nnnnnngann tggnnnnnnn acnnccnnnn cnncnntnnc cnncgtncnc nnnnnnnnnn gnntnnctga nncnnnnnat nnnnnnnnaa nnncnnnnnn gnnntnncnn nnnnntcnnt ccnncnnccn ntcnnnncnn angnnannnn nnnnnnnnnc nnnngnncnn nnannnncnn nnannnnnnn nnnnnnnnnn nacgnnnnna nnnnnnnnnn nnnnnnnnnn nnnnantnnn nnnnnnnana ccttcncncn nnannnangn nnnnnnnnnn ccnncncnnn nnnna
Expected Protein Sequence
MAHHHHHHMK GIIQGYYPSW VSYNHNLKDL NPNLNVVHMS FAKMDLSYDS IESIVGSPLL FKSLIGLEYI GLNEYFNDAM NLRKARPDII MLLSLGGETY HPSSFDSALN AVEKIANLVD ELGFDGIDVD YEPNGSFDGL NDKEKADFFV QYVTKLREYM CDDKLISISQ SSNGALSCIG FNDPKKICMD DE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag gaattattca aggttattat ccatcatggg tttcttataa tcataacttg aaagatttaa atcctaattt aaatgttgta catatgtctt ttgctaagat ggatttatcc tatgacagta tagagagtat tgttggttct cctttacttt tcaagtcatt aataggttta gaatatatag ggttaaacga atattttaat gatgccatga atttaagaaa agctcgtcca gatattatta tgcttttatc tcttggagga gaaacatatc atccaagttc atttgattct gctttaaatg ctgttgagaa aattgcaaat ttagttgatg aattaggatt tgatggaatc gatgttgatt atgagcctaa tggatctttt gatggtttga acgataaaga gaaagctgat tttttcgtac aatatgtaac aaaattgagg gaatatatgt gtgatgataa attaatttct atatctcaat catctaacgg agctttaagt tgtataggtt ttaatgatcc taagaaaata tgtatggatg atgaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PlfaA.18484.a.B6.GE36466
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 66
Validated AA Sequence
MAHHHHHHMK GIIQGYYPSW VSYNHNLKDL NPNLNVVHMS FAKMDLSYDS IESIVGSPLL FKSLIGLEYI GLNEYFNDAM NLRKARPDII MLLSLGGETY HPSSFDSALN AVEKIANLVD ELGFDGIDVD YEPNGSFDGL NDKEKADFFV QYVTKLREYM CDDKLISISQ SSNGALSCIG FNDPKKICMD DEAPYNSKYF NKPDVKKELL RAAQMASLEE QYI
Validated NT Sequence
ttnnnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgaaagga attattcaag gttattatcc atcatgggtt tcttataatc ataacttgaa agatttaaat cctaatttaa atgttgtaca tatgtctttt gctaagatgg atttatccta tgacagtata gagagtattg ttggttctcc tttacttttc aagtcattaa taggtttaga atatataggg ttaaacgaat attttaatga tgccatgaat ttaagaaaag ctcgtccaga tattattatg cttttatctc ttggaggaga aacatatcat ccaagttcat ttgattctgc tttaaatgct gttgagaaaa ttgcaaattt agttgatgaa ttaggatttg atggaatcga tgttgattat gagcctaatg gatcttttga tggtttgaac gataaagaga aagctgattt tttcgtacaa tatgtaacaa aattgaggga atatatgtgt gatgataaat taatttctat atctcaatca tctaacggag ctttaagttg tataggtttt aatgatccta agaaaatatg tatggatgat gaagcaccat ataattcaaa atatttcaat aaacctgatg taaaaaagga gctattaaga gcagcgcaaa tggcttcgct ggaggagcaa tatatttaat gaataattta aaggatatga ttgatatggt gtttgtacna acctttaatt atacnaattc tacagattcn acagttatga aagaattata tgattcttat gcttattatg gaaaaaatat gattatgtta ttataatggg atttacatta atgtttcctt cnaccccttt taatccaatg ataaatgctt gnaaatccat tggtgatttt gtaaagactg aaannaatta aataaaanag canangggtt tggatnnngg nnnttntctt nngatgntgc ncntnatgan nnngcnnnnn nnttngtnna nnnttannna nnannnnnnn nnnnnnnnnn ccc
Expected Protein Sequence
MAHHHHHHMK GIIQGYYPSW VSYNHNLKDL NPNLNVVHMS FAKMDLSYDS IESIVGSPLL FKSLIGLEYI GLNEYFNDAM NLRKARPDII MLLSLGGETY HPSSFDSALN AVEKIANLVD ELGFDGIDVD YEPNGSFDGL NDKEKADFFV QYVTKLREYM CDDKLISISQ SSNGALSCIG FNDPKKICMD DEAPYNSKYF NKPDVKKELL RAAQMASAGG AIYLMNNLKD MIDMVFVQTF NYTNSTDSTV MKELYDSYAY YGKKYDYVII MGFTLMFPST PFNPNDKMLV KSIGDFVKTE NKLNKRADGF GLWSLSSDNA AHNEQLAIEY FVESLH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag gaattattca aggttattat ccatcatggg tttcttataa tcataacttg aaagatttaa atcctaattt aaatgttgta catatgtctt ttgctaagat ggatttatcc tatgacagta tagagagtat tgttggttct cctttacttt tcaagtcatt aataggttta gaatatatag ggttaaacga atattttaat gatgccatga atttaagaaa agctcgtcca gatattatta tgcttttatc tcttggagga gaaacatatc atccaagttc atttgattct gctttaaatg ctgttgagaa aattgcaaat ttagttgatg aattaggatt tgatggaatc gatgttgatt atgagcctaa tggatctttt gatggtttga acgataaaga gaaagctgat tttttcgtac aatatgtaac aaaattgagg gaatatatgt gtgatgataa attaatttct atatctcaat catctaacgg agctttaagt tgtataggtt ttaatgatcc taagaaaata tgtatggatg atgaagcacc atataattca aaatatttca ataaacctga tgtaaaaaag gagctattaa gagcagcgca aatggcttca gctggaggag caatatattt aatgaataat ttaaaggata tgattgatat ggtgtttgta caaaccttta attatacaaa ttctacagat tcaacagtta tgaaagaatt atatgattct tatgcttatt atggaaaaaa atatgattat gttattataa tgggatttac attaatgttt ccttcaaccc cttttaatcc gaatgataaa atgcttgtaa aatccattgg tgattttgta aagactgaaa ataaattaaa taaaagagca gacgggtttg gattatggtc tttatcttct gataatgctg cacataatga acaattagca attgaatatt tcgtagaatc tttacattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for PlfaA.18484.a.B7.GE36467
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMR ARPGESRKNP REIIKTFKES GKGIIQGYYP SWVSYNHNLK DLNPNLNVVH MSFAKMDLSY DSIESIVGSP LLFKSLIGLE YIGLNEYFND AMNLRKARPD IIMLLSLGGE TYHPSSFDSA LNAVEKIANL VDELGFDGID VDYEPNGSFD GLNDKEKADF FVQYVTKLRE YMCDDKLISI SQSSNGALSC IGFNDPKKIC MDDEAPYNSK YFNKPDVKKE LLRAAQMASA GGAIYLMNNL KXMXDMVFVX TFNYTNSTDS TVMKELYDSY AYYGKNMIML L
Validated NT Sequence
ttgtnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgcgagca cgaccaggtg aatcaagaaa aaacccgaga gaaattataa aaacatttaa agagagtggg aaaggaatta ttcaaggtta ttatccatca tgggtttctt ataatcataa cttgaaagat ttaaatccta atttaaatgt tgtacatatg tcttttgcta agatggattt atcctatgac agtatagaga gtattgttgg ttctccttta cttttcaagt cattaatagg tttagaatat atagggttaa acgaatattt taatgatgcc atgaatttaa gaaaagctcg tccagatatt attatgcttt tatctcttgg aggagaaaca tatcatccaa gttcatttga ttctgcttta aatgctgttg agaaaattgc aaatttagtt gatgaattag gatttgatgg aatcgatgtt gattatgagc ctaatggatc ttttgatggt ttgaacgata aagagaaagc tgattttttc gtacaatatg taacaaaatt gagggaatat atgtgtgatg ataaattaat ttctatatct caatcatcta acggagcttt aagttgtata ggttttaatg atcctaagaa aatatgtatg gatgatgaag caccatataa ttcnaaatat ttcaataaac ctgatgtaaa aaaggagcta ttaagagcag cgcaaatggc ttcngctgga ggagcnatat atttaatgaa taatttaaag ganatgantg atatggtgtt tgtacnaacc tttaattata cnaattctac ngattcnaca gttatgaaag aattatatga ttcttatgct tattatggaa aaaatatgat tatgttatta taatgggatt tacattaatg tttccttcac nncttttaat ccnaatgann aaatgcttgn aaatccattg gtgattttgn aaagacngaa annnaattna nnnaaanagn nnannggttt ngattnnggn nntnanntnn nntnnnnnna nnnnnnnnnn nnnnncccnn nnnnnnaaaa aaaaaaaaaa aaannnnnnn nc
Expected Protein Sequence
MAHHHHHHMR ARPGESRKNP REIIKTFKES GKGIIQGYYP SWVSYNHNLK DLNPNLNVVH MSFAKMDLSY DSIESIVGSP LLFKSLIGLE YIGLNEYFND AMNLRKARPD IIMLLSLGGE TYHPSSFDSA LNAVEKIANL VDELGFDGID VDYEPNGSFD GLNDKEKADF FVQYVTKLRE YMCDDKLISI SQSSNGALSC IGFNDPKKIC MDDEAPYNSK YFNKPDVKKE LLRAAQMASA GGAIYLMNNL KDMIDMVFVQ TFNYTNSTDS TVMKELYDSY AYYGKKYDYV IIMGFTLMFP STPFNPNDKM LVKSIGDFVK TENKLNKRAD GFGLWSLSSD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgag cacgaccagg tgaatcaaga aaaaacccga gagaaattat aaaaacattt aaagagagtg ggaaaggaat tattcaaggt tattatccat catgggtttc ttataatcat aacttgaaag atttaaatcc taatttaaat gttgtacata tgtcttttgc taagatggat ttatcctatg acagtataga gagtattgtt ggttctcctt tacttttcaa gtcattaata ggtttagaat atatagggtt aaacgaatat tttaatgatg ccatgaattt aagaaaagct cgtccagata ttattatgct tttatctctt ggaggagaaa catatcatcc aagttcattt gattctgctt taaatgctgt tgagaaaatt gcaaatttag ttgatgaatt aggatttgat ggaatcgatg ttgattatga gcctaatgga tcttttgatg gtttgaacga taaagagaaa gctgattttt tcgtacaata tgtaacaaaa ttgagggaat atatgtgtga tgataaatta atttctatat ctcaatcatc taacggagct ttaagttgta taggttttaa tgatcctaag aaaatatgta tggatgatga agcaccatat aattcaaaat atttcaataa acctgatgta aaaaaggagc tattaagagc agcgcaaatg gcttcagctg gaggagcaat atatttaatg aataatttaa aggatatgat tgatatggtg tttgtacaaa cctttaatta tacaaattct acagattcaa cagttatgaa agaattatat gattcttatg cttattatgg aaaaaaatat gattatgtta ttataatggg atttacatta atgtttcctt caaccccttt taatccgaat gataaaatgc ttgtaaaatc cattggtgat tttgtaaaga ctgaaaataa attaaataaa agagcagacg ggtttggatt atggtcttta tcttctgatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat