PlfaA.18431.a

O-sialoglycoprotein endopeptidase, putative (EC 3.4.24.57)

CENTER ID: PlfaA.18431.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18431.a.B2.GE36231 Crowther superfamily domain Modbase/HHpred/XtalPred 1 168
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_0708300
RefSeq: XP_001348997.1
UniProt: Q8IC06

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAGSDARIY RCTFIKKEAV KKVIYRKYYR HKKIDTKIRK LRVSNEIKFT KKLASLNIDV PYIYFVDAKE KSLYFEYVKG CTINFILKNL KEYEPKIPIC VGMVLAKIHN GNIIHGDYTT SNLILRNSFI QQNNLLDLKN NNSPYNFSDL ENVKLCVIDF GLSFLSSSIE DKAVDLFVLL KTIKSFHSEF PSLEEDILEG YKMKSDNVND IFTKLEIVKQ RGRKRPMVG
NT Sequence
atgtctgcag gatctgatgc taggatctat cgatgcacgt ttattaaaaa ggaagcagtg aaaaaagtta tttacagaaa atattacaga cataaaaaaa ttgatacgaa aataaggaaa ttaagagtat ctaatgaaat taagtttaca aaaaaattag ctagtttaaa tattgatgtt ccatatattt attttgtcga tgctaaagaa aaaagtttat atttcgagta tgtgaaagga tgtaccataa attttatatt aaaaaattta aaagaatatg aacctaaaat acctatttgt gtaggtatgg tgttagcaaa aatacataat ggtaatataa tacatggaga ttatactacg tcaaatttaa ttttaagaaa ttcttttatt caacaaaata atctattaga cctaaagaat aataattctc catataattt tagtgacttg gaaaatgtca aattgtgtgt tatagatttc ggattatcct ttttatcctc atcaatagaa gataaagctg tcgatttgtt tgttctccta aaaaccatca aaagttttca tagtgaattt ccctccttgg aggaggatat tcttgaagga tacaaaatga agtctgacaa tgttaacgac atatttacaa aattagaaat agttaaacaa aggggaagaa aaagacctat ggttggatga
Details for PlfaA.18431.a.B2.GE36231
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 21kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS AGSDARIYRC TFIKKEAVKK VIYRKYYRHK KIDTKIRKLR VSNEIKFTKX LASLNIDVPY IYFVDAKEKS LYFEYVKGCT INFILKNLKE YEPKIPICVG MVLAKIHNGN IIHGDYTTSN LILRNSFIQQ NNLLDLKNNN SPYNFSDLEN VKLCVIDFGL SFLSSS
Validated NT Sequence
tttgtttact ttaagaagga ganataccat ggctcaccac caccaccacc atatgtctgc aggatctgat gctaggatct atcgatgcac gtttattaaa aaggaagcag tgaaaaaagt tatttacaga aaatattaca gacataaaaa aattgatacg aaaataagga aattaagagt atctaatgaa attaagttta caaaanaatt agctagttta aatattgatg ttccatatat ttattttgtc gatgctaaag aaaaaagttt atatttcgag tatgtgaaag gatgtaccat aaattttata ttaaaaaatt taaaagaata tgaacctaaa atacctattt gtgtaggtat ggtgttagca aaaatacata atggtaatat aatacatgga gattatacta cgtcaaattt aattttaaga aattctttta ttcaacaaaa taatctatta gacctaaaga ataataattc tccatataat tttagtgact tggaaaatgt caaattgtgt gttatagatt tcggattatc ctttttatcc tcatcataag tgagtaagat aggatccggc tgctannnnc ccccnaaagg annnannngn nnnaannnnn nnnntctnnn nnnnnnnnnn nnnnnatcnn nnccgagnnn annnnnnnnn gnnnnnnnng nncnnnnann tgagccannn naannnnnnn nnnnnnnnct cntangnngn ncntgcnnnn ncnngnnnan nnnnnnngac nacntgntcc ncgtntcncg annnnnnnnc nnnnnncnnc ncgnnnnnnc gannacanac ganccnnnnn nnnncncnnc ctccnnnnnn ncnnnnnnnt tcncncnnnn nttntc
Expected Protein Sequence
MAHHHHHHMS AGSDARIYRC TFIKKEAVKK VIYRKYYRHK KIDTKIRKLR VSNEIKFTKK LASLNIDVPY IYFVDAKEKS LYFEYVKGCT INFILKNLKE YEPKIPICVG MVLAKIHNGN IIHGDYTTSN LILRNSFIQQ NNLLDLKNNN SPYNFSDLEN VKLCVIDFGL SFLSSS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg caggatctga tgctaggatc tatcgatgca cgtttattaa aaaggaagca gtgaaaaaag ttatttacag aaaatattac agacataaaa aaattgatac gaaaataagg aaattaagag tatctaatga aattaagttt acaaaaaaat tagctagttt aaatattgat gttccatata tttattttgt cgatgctaaa gaaaaaagtt tatatttcga gtatgtgaaa ggatgtacca taaattttat attaaaaaat ttaaaagaat atgaacctaa aatacctatt tgtgtaggta tggtgttagc aaaaatacat aatggtaata taatacatgg agattatact acgtcaaatt taattttaag aaattctttt attcaacaaa ataatctatt agacctaaag aataataatt ctccatataa ttttagtgac ttggaaaatg tcaaattgtg tgttatagat ttcggattat cctttttatc ctcatcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at