PlfaA.18430.b

Sporozoite surface protein P36p (Sporozoite surface protein P52)

CENTER ID: PlfaA.18430.b
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.18430.b.B2.GE37854 soluble domain 38 458
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_0404500
RefSeq: XP_001351357.1
UniProt: Q8I1Y4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYVLVLIHMC YHFTMKRKKL FVYFIFLSFI INFNFNININ FVCSNVIQDV ISIGNVDICV VNVNSDEAQE CILNNEFGKL LLFVCNMNDA FSTTAKTHPE NCPSRAFVNQ SNPTENSPEV DTYSIYPNLF GTNENRLNDT YSLYSTPYSN MDIDFSCLCY GDKQDKVKHI MRINIKKTRK KIKGCDFGDN IPSKRDLTNS LSLNERSSCI IHAYSNDVLG INCFKKEINN SYNNNLELNP SNCFHDVYFG ADLILNSKNV IPNSRVIPDP SSDVKLSRNH SFSSYLILPN NLTENIKISC TCKRDEFVGT MIIYTKNINS LMFDNNNNNN DEEQIFQNKY MKKKEYKKDE GNEYDKKMNT DDNYINNEEH HNNNQYNNYE NKINNVNYNY DDISKYINEH YKNYDHEKNS KNSYKTNTNI HDQYDTYHYN NKYDLHSDRT RIRTRTFWQN LFGLSSSKYI LFNNFLILFI FLIYIYST
NT Sequence
atgtatgtat tggtgcttat tcatatgtgt taccatttta caatgaagag aaaaaagttg tttgtatatt ttattttttt gtcatttatt attaatttta attttaatat taatatcaat tttgtgtgta gcaatgtgat tcaagatgtt ataagcattg gtaatgtaga catctgtgta gtgaatgtaa attcagatga agctcaagaa tgcatattga ataatgaatt tgggaagttg ttattatttg tgtgtaatat gaatgatgct tttagtacta cggcaaaaac tcacccagaa aattgccctt ctagagcctt tgttaatcaa agtaatccaa ccgaaaattc ccccgaggtt gatacttata gtatttatcc gaatttattt ggtacgaacg aaaacagatt aaatgacaca tatagtttat atagtactcc ttattcaaat atggatattg attttagttg cttgtgttac ggagataaac aagacaaagt aaaacatata atgagaataa atataaaaaa aacaaggaaa aaaattaagg gttgtgattt tggtgataat ataccttcca aaagggatct aactaatagt ttaagtttga atgaacgatc tagttgtatt attcatgctt attcaaatga tgtattgggc ataaattgtt ttaaaaaaga aattaataat tcttataata ataatttaga attaaatcct agtaattgtt ttcatgatgt ttattttgga gctgatctta tattaaattc taaaaatgta atacctaatt ctagagttat ccctgaccct tcatcagatg tcaaattatc tagaaatcat tctttttctt cctatttaat attaccgaat aatttaactg aaaatattaa aattagctgt acatgtaaac gagatgaatt cgttggtacc atgatcattt acacaaaaaa tattaacagc ttaatgtttg ataataataa taataataat gatgaagaac agatttttca aaataaatat atgaaaaaaa aagaatataa aaaggatgaa ggaaatgaat atgataaaaa aatgaataca gatgataatt atataaataa tgaggaacat cataataaca atcaatataa taattatgaa aataaaatta ataatgtaaa ttataattat gatgatattt ccaaatatat aaatgaacat tataaaaatt atgaccatga aaaaaattct aaaaatagtt ataaaactaa tacaaatatt cacgatcaat atgatacata tcattataat aataaatatg atttacattc tgatagaaca agaatacgaa caagaacatt ctggcaaaat ctttttggat tatcttcttc taaatatata ttatttaata atttcttaat attattcata tttttaatat atatatattc aacataa
Details for PlfaA.18430.b.B2.GE37854
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/14/2014
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 59
Validated AA Sequence
MNDAFSTTAK THPENCPSRA FVNQSNPTEN SPEVDTYSIY PNLFGTNENR LNDTYSLYST PYSNMDIDFS CLCYGDKQDK VKHIMRINIK KTRKKIKGCD FGDNIPSKRD LTNSLSLNER SSCIIHAYSN DVLGINCFKK EINNSYNNNL ELNPSNCFHD VYFGADLILN SKNVIPNSRV IPDPSSDVKL SRNHSFSXYX ILPNNLXENI KISCTCKRDE FVGTMIIYXK NINSLMFXNN XXNNDEEQXF QINX
Validated NT Sequence
atatcaattt tgtgtgtagc aatgtgattc aagatgttat aagcattggt aatgtagaca tctgtgtagt gaatgtaaat tcagatgaag ctcaagaatg catattgaat aatgaatttg ggaagttgtt attatttgtg tgtaatatga atgatgcttt tagtactacg gcaaaaactc acccagaaaa ttgcccttct agagcctttg ttaatcaaag taatccaacc gaaaattccc ccgaggttga tacttatagt atttatccga atttatttgg tacgaacgaa aacagattaa atgacacata tagtttatat agtactcctt attcaaatat ggatattgat tttagttgct tgtgttacgg agataaacaa gacaaagtaa aacatataat gagaataaat ataaaaaaaa caaggaaaaa aattaagggt tgtgattttg gtgataatat accttccaaa agggatctaa ctaatagttt aagtttgaat gaacgatcta gttgtattat tcatgcttat tcaaatgatg tattgggcat aaattgtttt aaaaaagaaa ttaataattc ttataataat aatttagaat taaatcctag taattgtttt catgatgttt attttggagc tgatcttata ttaaattcta aaaatgtaat acctaattct agagttatcc ctgacccttc atcagatgtc aaattatcta gaaatcattc tttttcttnc tatttnatat taccgaataa tttanctgaa aatattaaaa ttagctgtac atgtaaacga gatgaattcg ttggtaccat gatcatttac ncaaaaaata ttaacagctt aatgtttgan aataatantn ataataatga tgaagaacag anttttcaaa taaatntntg aaaaaaanga nnnnaannna tgaannaann gannntgana aaaaatgnnt a
Expected Protein Sequence
MAHHHHHHNI NFVCSNVIQD VISIGNVDIC VVNVNSDEAQ ECILNNEFGK LLLFVCNMND AFSTTAKTHP ENCPSRAFVN QSNPTENSPE VDTYSIYPNL FGTNENRLND TYSLYSTPYS NMDIDFSCLC YGDKQDKVKH IMRINIKKTR KKIKGCDFGD NIPSKRDLTN SLSLNERSSC IIHAYSNDVL GINCFKKEIN NSYNNNLELN PSNCFHDVYF GADLILNSKN VIPNSRVIPD PSSDVKLSRN HSFSSYLILP NNLTENIKIS CTCKRDEFVG TMIIYTKNIN SLMFDNNNNN NDEEQIFQNK YMKKKEYKKD EGNEYDKKMN TDDNYINNEE HHNNNQYNNY ENKINNVNYN YDDISKYINE HYKNYDHEKN SKNSYKTNTN IHDQYDTYHY NNKYDLHSDR TRIRTRTFWQ NLFGLSSSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatatca attttgtgtg tagcaatgtg attcaagatg ttataagcat tggtaatgta gacatctgtg tagtgaatgt aaattcagat gaagctcaag aatgcatatt gaataatgaa tttgggaagt tgttattatt tgtgtgtaat atgaatgatg cttttagtac tacggcaaaa actcacccag aaaattgccc ttctagagcc tttgttaatc aaagtaatcc aaccgaaaat tcccccgagg ttgatactta tagtatttat ccgaatttat ttggtacgaa cgaaaacaga ttaaatgaca catatagttt atatagtact ccttattcaa atatggatat tgattttagt tgcttgtgtt acggagataa acaagacaaa gtaaaacata taatgagaat aaatataaaa aaaacaagga aaaaaattaa gggttgtgat tttggtgata atataccttc caaaagggat ctaactaata gtttaagttt gaatgaacga tctagttgta ttattcatgc ttattcaaat gatgtattgg gcataaattg ttttaaaaaa gaaattaata attcttataa taataattta gaattaaatc ctagtaattg ttttcatgat gtttattttg gagctgatct tatattaaat tctaaaaatg taatacctaa ttctagagtt atccctgacc cttcatcaga tgtcaaatta tctagaaatc attctttttc ttcctattta atattaccga ataatttaac tgaaaatatt aaaattagct gtacatgtaa acgagatgaa ttcgttggta ccatgatcat ttacacaaaa aatattaaca gcttaatgtt tgataataat aataataata atgatgaaga acagattttt caaaataaat atatgaaaaa aaaagaatat aaaaaggatg aaggaaatga atatgataaa aaaatgaata cagatgataa ttatataaat aatgaggaac atcataataa caatcaatat aataattatg aaaataaaat taataatgta aattataatt atgatgatat ttccaaatat ataaatgaac attataaaaa ttatgaccat gaaaaaaatt ctaaaaatag ttataaaact aatacaaata ttcacgatca atatgataca tatcattata ataataaata tgatttacat tctgatagaa caagaatacg aacaagaaca ttctggcaaa atctttttgg attatcttct tctaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t