PlfaA.17789.b
Plasmepsin X (PM10, PMX)
| CENTER ID: | PlfaA.17789.b |
| ORGANISM: | Plasmodium falciparum 3D7 |
| ASSOCIATED DISEASE: | Malaria |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PlfaA.17789.b.HE11.GE42299 | full length | 28 | 573 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PlfaA.17789.b.HE11.PD38304 | full length | 28 | 573 | ||
| PlfaA.17789.b.HE11.PD38305 | full length | 28 | 573 | ||
| PlfaA.17789.b.HE11.PD38318 | full length | 28 | 573 | ||
| PlfaA.17789.b.HE11.PD38319 | full length | 28 | 573 |
Structures
7RY7
DEPOSITED: 8/24/2021
DETERMINATION: XRay
CLONE: PlfaA.17789.b.HE11.GE42299
PROTEIN: PlfaA.17789.b.HE11.PD38363
Publications by SSGCID
Structures of Plasmepsin X from P. falciparum reveal a novel inactivation mechanism of the zymogen and molecular basis for binding of inhibitors in mature enzyme
Abendroth J, Edwards T, Lorimer D, Labaied M, Bhaumik P, Kesari P, Deshmukh A, Pahelkar N, Suryawanshi AB, Rathore I, Mishra V, Dupuis JH, Xiao H, Gustchina A, Yada RY, Wlodawer A
Protein Sci. - 2022
PMID: 35048450; PMCID: PMC8927862
PDB:
7RY7
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | PlasmoDB:PF3D7_0808200 |
| OrthoMCL: | OG5_133546 |
| RefSeq: | XP_001349441.1 |
| UniProt: | Q8IAS0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRISPLNTL FYLSLFFSYT FKGLKCTRIY KIGTKALPCS ECHDVFDCTG CLFEEKESSH VIPLKLNKKN PNDHKKLQKH HESLKLGDVK YYVNRGEGIS GSLGTSSGNT LDDMDLINEE INKKRTNAQL DEKNFLDFTT YNKNKAQDIS DHLSDIQKHV YEQDAQKGNK NFTNNENNSD NENNSDNENN SDNENNLDNE NNLDNENNSD NSSIEKNFIA LENKNATVEQ TKENIFLVPL KHLRDSQFVG ELLVGTPPQT VYPIFDTGST NVWVVTTACE EESCKKVRRY DPNKSKTFRR SFIEKNLHIV FGSGSISGSV GTDTFMLGKH LVRNQTFGLV ESESNNNKNG GDNIFDYISF EGIVGLGFPG MLSAGNIPFF DNLLKQNPNV DPQFSFYISP YDGKSTLIIG GISKSFYEGD IYMLPVLKES YWEVKLDELY IGKERICCDE ESYVIFDTGT SYNTMPSSQM KTFLNLIHST ACTEQNYKDI LKSYPIIKYV FGELIIELHP EEYMILNDDV CMPAYMQIDV PSERNHAYLL GSLSFMRNFF TVFVRGTESR PSMVGVARAK SKN
NT Sequence
atgaaacgca ttagccctct aaacaccctt ttttatttaa gtttattttt ttcatacaca tttaaagggt taaaatgcac cagaatatat aaaatcggaa cgaaagcgtt accgtgttct gagtgtcacg atgtatttga ttgtaccggg tgtttattcg aggaaaaaga atcttctcat gtgatacctt taaaattaaa caagaagaat ccaaacgatc ataaaaaatt acaaaaacat catgagtctc taaaattagg ggacgtaaaa tattatgtaa acagaggaga aggaatttcc ggaagtttag gaacgtcgtc tggtaataca ttagatgata tggatttaat aaatgaagaa ataaataaaa aacgaacaaa tgcacaatta gatgaaaaaa actttttaga ttttactacg tataataaaa ataaagcaca agatatatct gaccatttat ctgatattca gaaacatgtg tatgaacaag atgctcaaaa gggaaataaa aattttacaa ataatgaaaa caattcagat aatgaaaaca attcagataa tgaaaacaat tcagataatg aaaacaattt agataatgaa aacaatttag ataatgaaaa caattcagat aatagttcga ttgaaaaaaa ttttattgct ttagaaaata aaaacgctac agtagagcaa acaaaagaaa atatttttct agtaccctta aaacacttaa gagatagcca atttgtagga gaattattag ttggtacccc ccctcaaact gtatatccaa tatttgatac aggaagtaca aatgtatggg tagtgacaac agcttgtgaa gaagagtctt gtaaaaaagt ccgaagatat gatcctaata aatcaaaaac atttaggaga tcattcatag aaaaaaattt acacatagta tttggatccg gttctatatc aggttctgta ggtacagata catttatgtt aggaaagcac ctagtaagaa atcagacttt tggattagta gagagtgaat caaataataa taaaaatggt ggggataata tatttgatta tatatccttt gaaggtatag taggcttagg atttccagga atgttatcag ctgggaatat accatttttt gataatttat taaaacaaaa tccaaatgta gatcctcaat tttcttttta tatatctcct tatgatggga agtcaacttt aataattggt gggattagta aatcgtttta tgagggcgat atatacatgt tgccagtatt aaaagaatcc tactgggaag taaaattaga tgaattatat attggtaaag aaagaatatg ttgtgatgaa gaaagttacg ttatatttga cacaggtaca tcttataata caatgcctag tagtcagatg aaaacatttt tgaatctaat acattcaact gcatgtactg aacaaaacta taaagatata ttaaaatcat atcctataat taaatatgtt tttggtgagc taatcattga attacatcca gaagaatata tgattttaaa cgatgatgta tgtatgcctg cctacatgca aatcgatgta ccatccgaaa gaaaccatgc atatttatta ggcagtttat cttttatgag gaattttttt acggtatttg tacgaggcac agaaagtaga ccttctatgg ttggagtagc aagagcaaaa agtaaaaact aa
Details for PlfaA.17789.b.HE11.GE42299
| HARVESTED ON: | 1/15/2018 |
| SEQUENCED ON: | 3/16/2018 |
| EXPECTED MW: | 66kDa |
| OBSERVED MW: | 66kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Data Unavailable |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | HEK293-6E |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MEWSWVFLFF LSVTTGVHSR IYKIGTKALP CSECHDVFDC TGCLFEEKES SHVIPLKLNK KNPNDHKKLQ KHHESLKLGD VKYYVNRGEG ISGSLGTSSG NTLDDMDLIN EEINKKRTNA QLDEKNFLDF TTYNKNKAQD ISDHLSDIQK HVYEQDAQKG NKNFTNNENN SDNENNSDNE NNSDNENNLD NENNLDNENN SDNSSIEKNF IALENKNATV EQTKENIFLV PLKHLRDSQF VGELLVGTPP QTVYPIFDTG STNVWVVTTA CEEESCKKVR RYDPNKSKTF RRSFIEKNLH IVFGSGSISG SVGTDTFMLG KHLVRNQTFG LVESESNNNK NGGDNIFDYI SFEGIVGLGF PGMLSAGNIP FFDNLLKQNP NVDPQFSFYI SPYDGKSTLI IGGISKSFYE GDIYMLPVLK ESYWEVKLDE LYIGKERICC DEESYVIFDT GTSYNTMPSS QMKTFLNLIH STACTEQNYK DILKSYPIIK YVFGELIIEL HPEEYMILND DVCMPAYMQI DVPSERNHAY LLGSLSFMRN FFTVFVRGTE SRPSMVGVAR AKSKNENLYF QGSHHHHHHH H
Full NT Sequence (Expression Vector + Insert)
gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagagga tccatggaat ggtcctgggt gttcctgttc ttcctgagcg tgacaaccgg cgtgcacagc agaatctaca agatcggcac aaaggccctg ccttgcagcg agtgccacga tgtgtttgat tgcaccggct gcctgttcga ggaaaaagaa agcagccacg tgatccctct gaagctgaac aagaagaacc ccaacgacca caagaagctg cagaagcacc acgagagcct gaagctgggc gacgtgaagt actacgtgaa cagaggcgag ggcatctctg gcagcctggg cacatcttct ggcaacaccc tggacgacat ggacctgatc aacgaggaaa tcaacaagaa gcggacaaac gcccagctgg acgagaagaa cttcctggac ttcaccacct acaacaagaa caaggcccag gacatcagcg accacctgag cgacatccag aaacacgtgt acgagcagga cgcccagaag ggcaacaaga atttcaccaa caatgagaac aacagcgaca acgagaacaa ttccgataac gaaaacaata gtgataacga gaacaacctg gacaacgaaa acaacctcga taatgagaac aattctgaca acagcagcat cgagaaaaac tttatcgccc tggaaaacaa gaacgccacc gtggaacaga ccaaagaaaa catcttcctg gtgccactga agcacctgag agactcccag tttgtgggag agctgctcgt gggaacacct cctcagacag tgtaccccat cttcgacacc ggcagcacca atgtgtgggt tgtgaccacc gcctgcgagg aagagtcctg caagaaagtg cggagatacg accccaacaa gagcaagacc ttccggcgga gcttcatcga gaagaatctg cacatcgtgt tcggcagcgg cagcatcagt ggatctgtgg gcaccgatac cttcatgctg ggaaagcacc tcgtgcggaa tcagaccttt ggcctggtgg aaagcgagag caacaacaac aaaaacggcg gcgacaacat cttcgattac atcagctttg agggcatcgt cggcctgggc ttccctggaa tgctgtctgc cggcaacatc cctttcttcg acaacctgct gaagcagaac cctaacgtgg accctcagtt tagcttctac atcagcccct acgacggcaa gagcacactg atcatcggcg gcatcagcaa gagcttctac gagggcgaca tctacatgct gcccgtgctg aaagaaagct actgggaagt gaagctcgac gagctgtaca tcggcaaaga gcggatctgc tgcgacgagg aatcctacgt gatctttgat accgggacca gctacaacac catgcctagc agccagatga agacctttct gaacctgatc cacagcaccg cctgtaccga gcagaactat aaggacatcc tgaagtctta ccccatcatc aaatacgtgt tcggggagct gatcattgag ctgcaccccg aagagtacat gatcctgaac gacgacgtgt gcatgcccgc ctacatgcag atcgatgtgc cctccgagag aaaccacgcc tatctgctgg gcagcctgag cttcatgcgg aatttcttca ccgtgttcgt gcggggcacc gagtctagac cttctatggt tggagtggcc agagccaagt ccaagaacga gaacctgtac ttccaaggca gccaccacca tcatcaccat caccactgat aagcttgcta gcggccgctc gaggccggca aggccagatc ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactga accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgatg taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagc
Details for PlfaA.17789.b.HE11.PD38304
| PURIFICATION DATe: | 3/11/2018 |
| CONCENTRATION: | 4.85mg/ml |
| OBSERVED MW: | 66kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM tris pH 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol |
| EXPRESSION HOST: | HEK293-6E |
| VIAL COUNT (approx.): | 21 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MEWSWVFLFF LSVTTGVHSR IYKIGTKALP CSECHDVFDC TGCLFEEKES SHVIPLKLNK KNPNDHKKLQ KHHESLKLGD VKYYVNRGEG ISGSLGTSSG NTLDDMDLIN EEINKKRTNA QLDEKNFLDF TTYNKNKAQD ISDHLSDIQK HVYEQDAQKG NKNFTNNENN SDNENNSDNE NNSDNENNLD NENNLDNENN SDNSSIEKNF IALENKNATV EQTKENIFLV PLKHLRDSQF VGELLVGTPP QTVYPIFDTG STNVWVVTTA CEEESCKKVR RYDPNKSKTF RRSFIEKNLH IVFGSGSISG SVGTDTFMLG KHLVRNQTFG LVESESNNNK NGGDNIFDYI SFEGIVGLGF PGMLSAGNIP FFDNLLKQNP NVDPQFSFYI SPYDGKSTLI IGGISKSFYE GDIYMLPVLK ESYWEVKLDE LYIGKERICC DEESYVIFDT GTSYNTMPSS QMKTFLNLIH STACTEQNYK DILKSYPIIK YVFGELIIEL HPEEYMILND DVCMPAYMQI DVPSERNHAY LLGSLSFMRN FFTVFVRGTE SRPSMVGVAR AKSKNENLYF QGSHHHHHHH H
Full NT Sequence (Expression Vector + Insert)
GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCGAATTCC CTCTAGAGGA TCCATGGAAT GGTCCTGGGT GTTCCTGTTC TTCCTGAGCG TGACAACCGG CGTGCACAGC AGAATCTACA AGATCGGCAC AAAGGCCCTG CCTTGCAGCG AGTGCCACGA TGTGTTTGAT TGCACCGGCT GCCTGTTCGA GGAAAAAGAA AGCAGCCACG TGATCCCTCT GAAGCTGAAC AAGAAGAACC CCAACGACCA CAAGAAGCTG CAGAAGCACC ACGAGAGCCT GAAGCTGGGC GACGTGAAGT ACTACGTGAA CAGAGGCGAG GGCATCTCTG GCAGCCTGGG CACATCTTCT GGCAACACCC TGGACGACAT GGACCTGATC AACGAGGAAA TCAACAAGAA GCGGACAAAC GCCCAGCTGG ACGAGAAGAA CTTCCTGGAC TTCACCACCT ACAACAAGAA CAAGGCCCAG GACATCAGCG ACCACCTGAG CGACATCCAG AAACACGTGT ACGAGCAGGA CGCCCAGAAG GGCAACAAGA ATTTCACCAA CAATGAGAAC AACAGCGACA ACGAGAACAA TTCCGATAAC GAAAACAATA GTGATAACGA GAACAACCTG GACAACGAAA ACAACCTCGA TAATGAGAAC AATTCTGACA ACAGCAGCAT CGAGAAAAAC TTTATCGCCC TGGAAAACAA GAACGCCACC GTGGAACAGA CCAAAGAAAA CATCTTCCTG GTGCCACTGA AGCACCTGAG AGACTCCCAG TTTGTGGGAG AGCTGCTCGT GGGAACACCT CCTCAGACAG TGTACCCCAT CTTCGACACC GGCAGCACCA ATGTGTGGGT TGTGACCACC GCCTGCGAGG AAGAGTCCTG CAAGAAAGTG CGGAGATACG ACCCCAACAA GAGCAAGACC TTCCGGCGGA GCTTCATCGA GAAGAATCTG CACATCGTGT TCGGCAGCGG CAGCATCAGT GGATCTGTGG GCACCGATAC CTTCATGCTG GGAAAGCACC TCGTGCGGAA TCAGACCTTT GGCCTGGTGG AAAGCGAGAG CAACAACAAC AAAAACGGCG GCGACAACAT CTTCGATTAC ATCAGCTTTG AGGGCATCGT CGGCCTGGGC TTCCCTGGAA TGCTGTCTGC CGGCAACATC CCTTTCTTCG ACAACCTGCT GAAGCAGAAC CCTAACGTGG ACCCTCAGTT TAGCTTCTAC ATCAGCCCCT ACGACGGCAA GAGCACACTG ATCATCGGCG GCATCAGCAA GAGCTTCTAC GAGGGCGACA TCTACATGCT GCCCGTGCTG AAAGAAAGCT ACTGGGAAGT GAAGCTCGAC GAGCTGTACA TCGGCAAAGA GCGGATCTGC TGCGACGAGG AATCCTACGT GATCTTTGAT ACCGGGACCA GCTACAACAC CATGCCTAGC AGCCAGATGA AGACCTTTCT GAACCTGATC CACAGCACCG CCTGTACCGA GCAGAACTAT AAGGACATCC TGAAGTCTTA CCCCATCATC AAATACGTGT TCGGGGAGCT GATCATTGAG CTGCACCCCG AAGAGTACAT GATCCTGAAC GACGACGTGT GCATGCCCGC CTACATGCAG ATCGATGTGC CCTCCGAGAG AAACCACGCC TATCTGCTGG GCAGCCTGAG CTTCATGCGG AATTTCTTCA CCGTGTTCGT GCGGGGCACC GAGTCTAGAC CTTCTATGGT TGGAGTGGCC AGAGCCAAGT CCAAGAACGA GAACCTGTAC TTCCAAGGCA GCCACCACCA TCATCACCAT CACCACTGAT AAGCTTGCTA GCGGCCGCTC GAGGCCGGCA AGGCCAGATC CCCCGACCTC GACCTCTGGC TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC AAATCATTTG GTCGAGATCC CTCGGAGATC TCTAGCTAGA GGATCGATCC CCGCCCCGGA CGAACTAAAC CTGACTACGA CATCTCTGCC CCTTCTTCGC GGGGCAGTGC ATGTAATCCC TTCAGTTGGT TGGTACAACT TGCCAACTGA ACCCTAAACG GGTAGCATAT GCTTCCCGGG TAGTAGTATA TACTATCCAG ACTAACCCTA ATTCAATAGC ATATGTTACC CAACGGGAAG CATATGCTAT CGAATTAGGG TTAGTAAAAG GGTCCTAAGG AACAGCGATG TAGGTGGGCG GGCCAAGATA GGGGCGCGAT TGCTGCGATC TGGAGGACAA ATTACACACA CTTGCGCCTG AGCGCCAAGC ACAGGGTTGT TGGTCCTCAT ATTCACGAGG TCGCTGAGAG CACGGTGGGC TAATGTTGCC ATGGGTAGCA TATACTACCC AAATATCTGG ATAGCATATG CTATCCTAAT CTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT TTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT CTGTATCCGG GTAGCATATG CTATCCTAAT AGAGATTAGG GTAGTATATG CTATCCTAAT TTATATCTGG GTAGCATATA CTACCCAAAT ATCTGGATAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATCTGT ATCCGGGTAG CATATGCTAT CCTCATGATA AGCTGTCAAA CATGAGAATT AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGC
Details for PlfaA.17789.b.HE11.PD38305
| PURIFICATION DATe: | 3/11/2018 |
| CONCENTRATION: | 5.64mg/ml |
| OBSERVED MW: | 66kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM tris pH 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol |
| EXPRESSION HOST: | HEK293-6E |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MEWSWVFLFF LSVTTGVHSR IYKIGTKALP CSECHDVFDC TGCLFEEKES SHVIPLKLNK KNPNDHKKLQ KHHESLKLGD VKYYVNRGEG ISGSLGTSSG NTLDDMDLIN EEINKKRTNA QLDEKNFLDF TTYNKNKAQD ISDHLSDIQK HVYEQDAQKG NKNFTNNENN SDNENNSDNE NNSDNENNLD NENNLDNENN SDNSSIEKNF IALENKNATV EQTKENIFLV PLKHLRDSQF VGELLVGTPP QTVYPIFDTG STNVWVVTTA CEEESCKKVR RYDPNKSKTF RRSFIEKNLH IVFGSGSISG SVGTDTFMLG KHLVRNQTFG LVESESNNNK NGGDNIFDYI SFEGIVGLGF PGMLSAGNIP FFDNLLKQNP NVDPQFSFYI SPYDGKSTLI IGGISKSFYE GDIYMLPVLK ESYWEVKLDE LYIGKERICC DEESYVIFDT GTSYNTMPSS QMKTFLNLIH STACTEQNYK DILKSYPIIK YVFGELIIEL HPEEYMILND DVCMPAYMQI DVPSERNHAY LLGSLSFMRN FFTVFVRGTE SRPSMVGVAR AKSKNENLYF QGSHHHHHHH H
Full NT Sequence (Expression Vector + Insert)
GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCGAATTCC CTCTAGAGGA TCCATGGAAT GGTCCTGGGT GTTCCTGTTC TTCCTGAGCG TGACAACCGG CGTGCACAGC AGAATCTACA AGATCGGCAC AAAGGCCCTG CCTTGCAGCG AGTGCCACGA TGTGTTTGAT TGCACCGGCT GCCTGTTCGA GGAAAAAGAA AGCAGCCACG TGATCCCTCT GAAGCTGAAC AAGAAGAACC CCAACGACCA CAAGAAGCTG CAGAAGCACC ACGAGAGCCT GAAGCTGGGC GACGTGAAGT ACTACGTGAA CAGAGGCGAG GGCATCTCTG GCAGCCTGGG CACATCTTCT GGCAACACCC TGGACGACAT GGACCTGATC AACGAGGAAA TCAACAAGAA GCGGACAAAC GCCCAGCTGG ACGAGAAGAA CTTCCTGGAC TTCACCACCT ACAACAAGAA CAAGGCCCAG GACATCAGCG ACCACCTGAG CGACATCCAG AAACACGTGT ACGAGCAGGA CGCCCAGAAG GGCAACAAGA ATTTCACCAA CAATGAGAAC AACAGCGACA ACGAGAACAA TTCCGATAAC GAAAACAATA GTGATAACGA GAACAACCTG GACAACGAAA ACAACCTCGA TAATGAGAAC AATTCTGACA ACAGCAGCAT CGAGAAAAAC TTTATCGCCC TGGAAAACAA GAACGCCACC GTGGAACAGA CCAAAGAAAA CATCTTCCTG GTGCCACTGA AGCACCTGAG AGACTCCCAG TTTGTGGGAG AGCTGCTCGT GGGAACACCT CCTCAGACAG TGTACCCCAT CTTCGACACC GGCAGCACCA ATGTGTGGGT TGTGACCACC GCCTGCGAGG AAGAGTCCTG CAAGAAAGTG CGGAGATACG ACCCCAACAA GAGCAAGACC TTCCGGCGGA GCTTCATCGA GAAGAATCTG CACATCGTGT TCGGCAGCGG CAGCATCAGT GGATCTGTGG GCACCGATAC CTTCATGCTG GGAAAGCACC TCGTGCGGAA TCAGACCTTT GGCCTGGTGG AAAGCGAGAG CAACAACAAC AAAAACGGCG GCGACAACAT CTTCGATTAC ATCAGCTTTG AGGGCATCGT CGGCCTGGGC TTCCCTGGAA TGCTGTCTGC CGGCAACATC CCTTTCTTCG ACAACCTGCT GAAGCAGAAC CCTAACGTGG ACCCTCAGTT TAGCTTCTAC ATCAGCCCCT ACGACGGCAA GAGCACACTG ATCATCGGCG GCATCAGCAA GAGCTTCTAC GAGGGCGACA TCTACATGCT GCCCGTGCTG AAAGAAAGCT ACTGGGAAGT GAAGCTCGAC GAGCTGTACA TCGGCAAAGA GCGGATCTGC TGCGACGAGG AATCCTACGT GATCTTTGAT ACCGGGACCA GCTACAACAC CATGCCTAGC AGCCAGATGA AGACCTTTCT GAACCTGATC CACAGCACCG CCTGTACCGA GCAGAACTAT AAGGACATCC TGAAGTCTTA CCCCATCATC AAATACGTGT TCGGGGAGCT GATCATTGAG CTGCACCCCG AAGAGTACAT GATCCTGAAC GACGACGTGT GCATGCCCGC CTACATGCAG ATCGATGTGC CCTCCGAGAG AAACCACGCC TATCTGCTGG GCAGCCTGAG CTTCATGCGG AATTTCTTCA CCGTGTTCGT GCGGGGCACC GAGTCTAGAC CTTCTATGGT TGGAGTGGCC AGAGCCAAGT CCAAGAACGA GAACCTGTAC TTCCAAGGCA GCCACCACCA TCATCACCAT CACCACTGAT AAGCTTGCTA GCGGCCGCTC GAGGCCGGCA AGGCCAGATC CCCCGACCTC GACCTCTGGC TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC AAATCATTTG GTCGAGATCC CTCGGAGATC TCTAGCTAGA GGATCGATCC CCGCCCCGGA CGAACTAAAC CTGACTACGA CATCTCTGCC CCTTCTTCGC GGGGCAGTGC ATGTAATCCC TTCAGTTGGT TGGTACAACT TGCCAACTGA ACCCTAAACG GGTAGCATAT GCTTCCCGGG TAGTAGTATA TACTATCCAG ACTAACCCTA ATTCAATAGC ATATGTTACC CAACGGGAAG CATATGCTAT CGAATTAGGG TTAGTAAAAG GGTCCTAAGG AACAGCGATG TAGGTGGGCG GGCCAAGATA GGGGCGCGAT TGCTGCGATC TGGAGGACAA ATTACACACA CTTGCGCCTG AGCGCCAAGC ACAGGGTTGT TGGTCCTCAT ATTCACGAGG TCGCTGAGAG CACGGTGGGC TAATGTTGCC ATGGGTAGCA TATACTACCC AAATATCTGG ATAGCATATG CTATCCTAAT CTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT TTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT CTGTATCCGG GTAGCATATG CTATCCTAAT AGAGATTAGG GTAGTATATG CTATCCTAAT TTATATCTGG GTAGCATATA CTACCCAAAT ATCTGGATAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATCTGT ATCCGGGTAG CATATGCTAT CCTCATGATA AGCTGTCAAA CATGAGAATT AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGC
Details for PlfaA.17789.b.HE11.PD38318
| PURIFICATION DATe: | 6/13/2018 |
| CONCENTRATION: | 5.42mg/ml |
| OBSERVED MW: | 66kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25mM Tris pH 8.0, 200mM NaCl, 1mM TCEP, 1% Glycerol |
| EXPRESSION HOST: | HEK293-6E |
| VIAL COUNT (approx.): | 37 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MEWSWVFLFF LSVTTGVHSR IYKIGTKALP CSECHDVFDC TGCLFEEKES SHVIPLKLNK KNPNDHKKLQ KHHESLKLGD VKYYVNRGEG ISGSLGTSSG NTLDDMDLIN EEINKKRTNA QLDEKNFLDF TTYNKNKAQD ISDHLSDIQK HVYEQDAQKG NKNFTNNENN SDNENNSDNE NNSDNENNLD NENNLDNENN SDNSSIEKNF IALENKNATV EQTKENIFLV PLKHLRDSQF VGELLVGTPP QTVYPIFDTG STNVWVVTTA CEEESCKKVR RYDPNKSKTF RRSFIEKNLH IVFGSGSISG SVGTDTFMLG KHLVRNQTFG LVESESNNNK NGGDNIFDYI SFEGIVGLGF PGMLSAGNIP FFDNLLKQNP NVDPQFSFYI SPYDGKSTLI IGGISKSFYE GDIYMLPVLK ESYWEVKLDE LYIGKERICC DEESYVIFDT GTSYNTMPSS QMKTFLNLIH STACTEQNYK DILKSYPIIK YVFGELIIEL HPEEYMILND DVCMPAYMQI DVPSERNHAY LLGSLSFMRN FFTVFVRGTE SRPSMVGVAR AKSKNENLYF QGSHHHHHHH H
Full NT Sequence (Expression Vector + Insert)
GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCGAATTCC CTCTAGAGGA TCCATGGAAT GGTCCTGGGT GTTCCTGTTC TTCCTGAGCG TGACAACCGG CGTGCACAGC AGAATCTACA AGATCGGCAC AAAGGCCCTG CCTTGCAGCG AGTGCCACGA TGTGTTTGAT TGCACCGGCT GCCTGTTCGA GGAAAAAGAA AGCAGCCACG TGATCCCTCT GAAGCTGAAC AAGAAGAACC CCAACGACCA CAAGAAGCTG CAGAAGCACC ACGAGAGCCT GAAGCTGGGC GACGTGAAGT ACTACGTGAA CAGAGGCGAG GGCATCTCTG GCAGCCTGGG CACATCTTCT GGCAACACCC TGGACGACAT GGACCTGATC AACGAGGAAA TCAACAAGAA GCGGACAAAC GCCCAGCTGG ACGAGAAGAA CTTCCTGGAC TTCACCACCT ACAACAAGAA CAAGGCCCAG GACATCAGCG ACCACCTGAG CGACATCCAG AAACACGTGT ACGAGCAGGA CGCCCAGAAG GGCAACAAGA ATTTCACCAA CAATGAGAAC AACAGCGACA ACGAGAACAA TTCCGATAAC GAAAACAATA GTGATAACGA GAACAACCTG GACAACGAAA ACAACCTCGA TAATGAGAAC AATTCTGACA ACAGCAGCAT CGAGAAAAAC TTTATCGCCC TGGAAAACAA GAACGCCACC GTGGAACAGA CCAAAGAAAA CATCTTCCTG GTGCCACTGA AGCACCTGAG AGACTCCCAG TTTGTGGGAG AGCTGCTCGT GGGAACACCT CCTCAGACAG TGTACCCCAT CTTCGACACC GGCAGCACCA ATGTGTGGGT TGTGACCACC GCCTGCGAGG AAGAGTCCTG CAAGAAAGTG CGGAGATACG ACCCCAACAA GAGCAAGACC TTCCGGCGGA GCTTCATCGA GAAGAATCTG CACATCGTGT TCGGCAGCGG CAGCATCAGT GGATCTGTGG GCACCGATAC CTTCATGCTG GGAAAGCACC TCGTGCGGAA TCAGACCTTT GGCCTGGTGG AAAGCGAGAG CAACAACAAC AAAAACGGCG GCGACAACAT CTTCGATTAC ATCAGCTTTG AGGGCATCGT CGGCCTGGGC TTCCCTGGAA TGCTGTCTGC CGGCAACATC CCTTTCTTCG ACAACCTGCT GAAGCAGAAC CCTAACGTGG ACCCTCAGTT TAGCTTCTAC ATCAGCCCCT ACGACGGCAA GAGCACACTG ATCATCGGCG GCATCAGCAA GAGCTTCTAC GAGGGCGACA TCTACATGCT GCCCGTGCTG AAAGAAAGCT ACTGGGAAGT GAAGCTCGAC GAGCTGTACA TCGGCAAAGA GCGGATCTGC TGCGACGAGG AATCCTACGT GATCTTTGAT ACCGGGACCA GCTACAACAC CATGCCTAGC AGCCAGATGA AGACCTTTCT GAACCTGATC CACAGCACCG CCTGTACCGA GCAGAACTAT AAGGACATCC TGAAGTCTTA CCCCATCATC AAATACGTGT TCGGGGAGCT GATCATTGAG CTGCACCCCG AAGAGTACAT GATCCTGAAC GACGACGTGT GCATGCCCGC CTACATGCAG ATCGATGTGC CCTCCGAGAG AAACCACGCC TATCTGCTGG GCAGCCTGAG CTTCATGCGG AATTTCTTCA CCGTGTTCGT GCGGGGCACC GAGTCTAGAC CTTCTATGGT TGGAGTGGCC AGAGCCAAGT CCAAGAACGA GAACCTGTAC TTCCAAGGCA GCCACCACCA TCATCACCAT CACCACTGAT AAGCTTGCTA GCGGCCGCTC GAGGCCGGCA AGGCCAGATC CCCCGACCTC GACCTCTGGC TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC AAATCATTTG GTCGAGATCC CTCGGAGATC TCTAGCTAGA GGATCGATCC CCGCCCCGGA CGAACTAAAC CTGACTACGA CATCTCTGCC CCTTCTTCGC GGGGCAGTGC ATGTAATCCC TTCAGTTGGT TGGTACAACT TGCCAACTGA ACCCTAAACG GGTAGCATAT GCTTCCCGGG TAGTAGTATA TACTATCCAG ACTAACCCTA ATTCAATAGC ATATGTTACC CAACGGGAAG CATATGCTAT CGAATTAGGG TTAGTAAAAG GGTCCTAAGG AACAGCGATG TAGGTGGGCG GGCCAAGATA GGGGCGCGAT TGCTGCGATC TGGAGGACAA ATTACACACA CTTGCGCCTG AGCGCCAAGC ACAGGGTTGT TGGTCCTCAT ATTCACGAGG TCGCTGAGAG CACGGTGGGC TAATGTTGCC ATGGGTAGCA TATACTACCC AAATATCTGG ATAGCATATG CTATCCTAAT CTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT TTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT CTGTATCCGG GTAGCATATG CTATCCTAAT AGAGATTAGG GTAGTATATG CTATCCTAAT TTATATCTGG GTAGCATATA CTACCCAAAT ATCTGGATAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATCTGT ATCCGGGTAG CATATGCTAT CCTCATGATA AGCTGTCAAA CATGAGAATT AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGC
Details for PlfaA.17789.b.HE11.PD38319
| PURIFICATION DATe: | 6/13/2018 |
| CONCENTRATION: | 5.45mg/ml |
| OBSERVED MW: | 66kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25mM Tris pH 8.0, 200mM NaCl, 1mM TCEP, 1% Glycerol |
| EXPRESSION HOST: | HEK293-6E |
| VIAL COUNT (approx.): | 24 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MEWSWVFLFF LSVTTGVHSR IYKIGTKALP CSECHDVFDC TGCLFEEKES SHVIPLKLNK KNPNDHKKLQ KHHESLKLGD VKYYVNRGEG ISGSLGTSSG NTLDDMDLIN EEINKKRTNA QLDEKNFLDF TTYNKNKAQD ISDHLSDIQK HVYEQDAQKG NKNFTNNENN SDNENNSDNE NNSDNENNLD NENNLDNENN SDNSSIEKNF IALENKNATV EQTKENIFLV PLKHLRDSQF VGELLVGTPP QTVYPIFDTG STNVWVVTTA CEEESCKKVR RYDPNKSKTF RRSFIEKNLH IVFGSGSISG SVGTDTFMLG KHLVRNQTFG LVESESNNNK NGGDNIFDYI SFEGIVGLGF PGMLSAGNIP FFDNLLKQNP NVDPQFSFYI SPYDGKSTLI IGGISKSFYE GDIYMLPVLK ESYWEVKLDE LYIGKERICC DEESYVIFDT GTSYNTMPSS QMKTFLNLIH STACTEQNYK DILKSYPIIK YVFGELIIEL HPEEYMILND DVCMPAYMQI DVPSERNHAY LLGSLSFMRN FFTVFVRGTE SRPSMVGVAR AKSKNENLYF QGSHHHHHHH H
Full NT Sequence (Expression Vector + Insert)
GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCGAATTCC CTCTAGAGGA TCCATGGAAT GGTCCTGGGT GTTCCTGTTC TTCCTGAGCG TGACAACCGG CGTGCACAGC AGAATCTACA AGATCGGCAC AAAGGCCCTG CCTTGCAGCG AGTGCCACGA TGTGTTTGAT TGCACCGGCT GCCTGTTCGA GGAAAAAGAA AGCAGCCACG TGATCCCTCT GAAGCTGAAC AAGAAGAACC CCAACGACCA CAAGAAGCTG CAGAAGCACC ACGAGAGCCT GAAGCTGGGC GACGTGAAGT ACTACGTGAA CAGAGGCGAG GGCATCTCTG GCAGCCTGGG CACATCTTCT GGCAACACCC TGGACGACAT GGACCTGATC AACGAGGAAA TCAACAAGAA GCGGACAAAC GCCCAGCTGG ACGAGAAGAA CTTCCTGGAC TTCACCACCT ACAACAAGAA CAAGGCCCAG GACATCAGCG ACCACCTGAG CGACATCCAG AAACACGTGT ACGAGCAGGA CGCCCAGAAG GGCAACAAGA ATTTCACCAA CAATGAGAAC AACAGCGACA ACGAGAACAA TTCCGATAAC GAAAACAATA GTGATAACGA GAACAACCTG GACAACGAAA ACAACCTCGA TAATGAGAAC AATTCTGACA ACAGCAGCAT CGAGAAAAAC TTTATCGCCC TGGAAAACAA GAACGCCACC GTGGAACAGA CCAAAGAAAA CATCTTCCTG GTGCCACTGA AGCACCTGAG AGACTCCCAG TTTGTGGGAG AGCTGCTCGT GGGAACACCT CCTCAGACAG TGTACCCCAT CTTCGACACC GGCAGCACCA ATGTGTGGGT TGTGACCACC GCCTGCGAGG AAGAGTCCTG CAAGAAAGTG CGGAGATACG ACCCCAACAA GAGCAAGACC TTCCGGCGGA GCTTCATCGA GAAGAATCTG CACATCGTGT TCGGCAGCGG CAGCATCAGT GGATCTGTGG GCACCGATAC CTTCATGCTG GGAAAGCACC TCGTGCGGAA TCAGACCTTT GGCCTGGTGG AAAGCGAGAG CAACAACAAC AAAAACGGCG GCGACAACAT CTTCGATTAC ATCAGCTTTG AGGGCATCGT CGGCCTGGGC TTCCCTGGAA TGCTGTCTGC CGGCAACATC CCTTTCTTCG ACAACCTGCT GAAGCAGAAC CCTAACGTGG ACCCTCAGTT TAGCTTCTAC ATCAGCCCCT ACGACGGCAA GAGCACACTG ATCATCGGCG GCATCAGCAA GAGCTTCTAC GAGGGCGACA TCTACATGCT GCCCGTGCTG AAAGAAAGCT ACTGGGAAGT GAAGCTCGAC GAGCTGTACA TCGGCAAAGA GCGGATCTGC TGCGACGAGG AATCCTACGT GATCTTTGAT ACCGGGACCA GCTACAACAC CATGCCTAGC AGCCAGATGA AGACCTTTCT GAACCTGATC CACAGCACCG CCTGTACCGA GCAGAACTAT AAGGACATCC TGAAGTCTTA CCCCATCATC AAATACGTGT TCGGGGAGCT GATCATTGAG CTGCACCCCG AAGAGTACAT GATCCTGAAC GACGACGTGT GCATGCCCGC CTACATGCAG ATCGATGTGC CCTCCGAGAG AAACCACGCC TATCTGCTGG GCAGCCTGAG CTTCATGCGG AATTTCTTCA CCGTGTTCGT GCGGGGCACC GAGTCTAGAC CTTCTATGGT TGGAGTGGCC AGAGCCAAGT CCAAGAACGA GAACCTGTAC TTCCAAGGCA GCCACCACCA TCATCACCAT CACCACTGAT AAGCTTGCTA GCGGCCGCTC GAGGCCGGCA AGGCCAGATC CCCCGACCTC GACCTCTGGC TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC AAATCATTTG GTCGAGATCC CTCGGAGATC TCTAGCTAGA GGATCGATCC CCGCCCCGGA CGAACTAAAC CTGACTACGA CATCTCTGCC CCTTCTTCGC GGGGCAGTGC ATGTAATCCC TTCAGTTGGT TGGTACAACT TGCCAACTGA ACCCTAAACG GGTAGCATAT GCTTCCCGGG TAGTAGTATA TACTATCCAG ACTAACCCTA ATTCAATAGC ATATGTTACC CAACGGGAAG CATATGCTAT CGAATTAGGG TTAGTAAAAG GGTCCTAAGG AACAGCGATG TAGGTGGGCG GGCCAAGATA GGGGCGCGAT TGCTGCGATC TGGAGGACAA ATTACACACA CTTGCGCCTG AGCGCCAAGC ACAGGGTTGT TGGTCCTCAT ATTCACGAGG TCGCTGAGAG CACGGTGGGC TAATGTTGCC ATGGGTAGCA TATACTACCC AAATATCTGG ATAGCATATG CTATCCTAAT CTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT TTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT CTGTATCCGG GTAGCATATG CTATCCTAAT AGAGATTAGG GTAGTATATG CTATCCTAAT TTATATCTGG GTAGCATATA CTACCCAAAT ATCTGGATAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATCTGT ATCCGGGTAG CATATGCTAT CCTCATGATA AGCTGTCAAA CATGAGAATT AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGC