SSGCID: Target Page

PlfaA.17143.a

Alpha-tubulin ii

CENTER ID:	PlfaA.17143.a
ORGANISM:	Plasmodium falciparum 3D7
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlfaA.17143.a.B3.GE35248	C-term domain		246	440

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PF3D7_0422300
RefSeq:	XP_001351526.1
UniProt:	Q8IFP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MREVISIHVG QAGIQIGNAC WELFCLEHGI QPDGQMPSDQ VVAGGDDAFN TFFSETGAGK HVPRCVFVDL EPTVVDEVRT GTYRQLFHPE QLISGKEDAA NNFARGHYTI GKEIVDVCLD RVRKLADNCT GLQGFLMFNA VGGGTGSGLG CLLLERLAID YGKKSKLNFC SWPSPQVSTA VVEPYNSVLS THSLLEHTDV AIMLDNEAIY DICKKNLDIE RPTYTNLNRL IAQVISSLTA SLRFDGALNV DVTEFQTNLV PYPRIHFMLS SYAPIISAEK AYHEQLSVSE ITNSAFEPAS MMAKCDPRHG KYMACCLMYR GDVVPKDVNA AVATIKTKRS IQFVDWCPTG FKCGINYQPP TVVPGGDLAK VMRAVCMISN STAIAEVFSR MDQKFDLMYA KRAFVHWYVG EGMEEGEFSE AREDLAALEK DYEEVGIESN DGEGEDEGYE

NT Sequence

atgagagaag tcattagtat tcatgttgga caggctggta ttcaaatagg aaatgcttgc tgggaattgt tttgccttga acatggaatt caaccggatg ggcagatgcc aagtgaccaa gtcgttgctg gtggtgatga tgcctttaat acatttttct cagaaacggg agctggaaaa catgtaccac gttgtgtgtt cgttgattta gaacccaccg tcgttgacga agttcgaaca ggaacgtatc gtcagctgtt tcaccctgaa caactaatat ctggaaaaga ggatgcagca aataatttcg caaggggaca ttataccata ggaaaagaaa ttgttgatgt atgtttggat agggttcgaa agttggctga taattgcact ggattacaag gatttttgat gtttaatgca gtaggtggag gtacaggtag tggtcttggt tgtttattat tagaaaggtt ggcaatagat tatggaaaga aatcaaaatt aaatttttgt tcgtggccat ctcctcaagt atcgacagct gttgtagagc cttataattc tgtattatca acacattcat tgttagaaca tacagatgtg gcaattatgc tcgataacga agcaatatat gatatatgta agaaaaattt agatatagaa aggccaacct atactaactt gaatagattg attgctcaag ttatctcttc attaacagca tctttaagat ttgatggtgc tttgaatgtt gatgtaacag aatttcagac taatttagta ccatatccta gaattcactt tatgttatca tcatatgctc caatcataag tgctgagaag gcatatcacg agcaattgtc ggtttctgaa ataacgaatt ctgcctttga gcctgcatct atgatggcaa agtgtgatcc cagacatgga aaatatatgg cttgttgttt aatgtataga ggagatgtag taccaaagga tgttaatgct gccgtcgcaa ctattaagac taagagatct atacaattcg ttgattggtg tcctacggga tttaaatgtg gaatcaatta tcagccccct accgtggttc caggaggaga tttagcaaaa gttatgagag ctgtttgcat gatcagcaac tcaacagcaa ttgccgaagt attctcacga atggaccaaa aatttgattt gatgtatgcg aaaagggctt tcgttcattg gtatgtaggt gaaggtatgg aagaaggaga atttagtgaa gctagagagg atttggctgc cttggaaaag gattatgaag aggtaggaat agaatcgaat gatggagagg gagaagatga gggatatgaa tga

Details for PlfaA.17143.a.B3.GE35248

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	22kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG ALNVDVTEFQ TNLVPYPRIH FMLSSYAPII SAEKAYHEQL SVSEITNSAF EPASMMAKCD PRHGKYMACC LMYRGDVVPK DVNAAVATIK TKRSIQFVDW CPTGFKCGIN YQPPTVVPGG DLAKVMRAVC MISNSTAIAE VFSRMDQKFD LMYAKRAFVH WYVGEGMEEG EFSEAREDLA ALEKDYEEVG IESN

Validated NT Sequence

ttntnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgggtgct ttgaatgttg atgtaacaga atttcagact aatttagtac catatcctag aattcacttt atgttatcat catatgctcc aatcataagt gctgagaagg catatcacga gcaattgtcg gtttctgaaa taacgaattc tgcctttgag cctgcatcta tgatggcaaa gtgtgatccc agacatggaa aatatatggc ttgttgttta atgtatagag gagatgtagt accaaaggat gttaatgctg ccgtcgcaac tattaagact aagagatcta tacaattcgt tgattggtgt cctacgggat ttaaatgtgg aatcaattat cagcccccta ccgtggttcc aggaggagat ttagcaaaag ttatgagagc tgtttgcatg atcagcaact caacagcaat tgccgaagta ttctcacgaa tggaccaaaa atttgatttg atgtatgcga aaagggcttt cgttcattgg tatgtaggtg aaggtatgga agaaggagaa tttagtgaag ctagagagga tttggctgcc ttggaaaagg attatgaaga ggtaggaata gaatcgaatt aagtgagtaa gataggatcc ggctgcnnnn nnccccccnn nnnanannnn nnnnnnnnnn nnnnnnncnn nnnnnnnnnn nngngggtng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtg ctttgaatgt tgatgtaaca gaatttcaga ctaatttagt accatatcct agaattcact ttatgttatc atcatatgct ccaatcataa gtgctgagaa ggcatatcac gagcaattgt cggtttctga aataacgaat tctgcctttg agcctgcatc tatgatggca aagtgtgatc ccagacatgg aaaatatatg gcttgttgtt taatgtatag aggagatgta gtaccaaagg atgttaatgc tgccgtcgca actattaaga ctaagagatc tatacaattc gttgattggt gtcctacggg atttaaatgt ggaatcaatt atcagccccc taccgtggtt ccaggaggag atttagcaaa agttatgaga gctgtttgca tgatcagcaa ctcaacagca attgccgaag tattctcacg aatggaccaa aaatttgatt tgatgtatgc gaaaagggct ttcgttcatt ggtatgtagg tgaaggtatg gaagaaggag aatttagtga agctagagag gatttggctg ccttggaaaa ggattatgaa gaggtaggaa tagaatcgaa ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat