PlfaA.10272.a

Hexokinase (HK)

CENTER ID: PlfaA.10272.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.10272.a.B1.GE37853 full length 1 493
PlfaA.10272.a.B11.GE38824 full length 1 493
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_0624000
RefSeq: XP_966222.1
UniProt: C6KT76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEYDIAKND VTYTKLDTIE CDIPINEELS WRINKFVNQL RISYSTLEEF VDNFVYELKK GLEAHRKHPN LWIPHECSFK MLDSCIANIP TGQEKGTYYA IDFGGTNFRA VRASLDGKGK IKRDQETYSL KFTGSYSHEK GLLDKHATAS QLFDHFAERI KYIMGEFNDL DNKEVKSVGF TFSFPCTSPS INCSILIDWT KGFETGRATN DPVEGRDVCK LMNDAFVRAA IPAKVCCVLN DAVGTLMSCA YQKGRGTPPC YIGIILGTGS NGCYYEPEWK KYKYAGKIIN IEFGNFDKDL PTSPIDLVMD WYSANRSRQL FEKMISGAYL GEIVRRFMVN VLQSACSKKM WISDSFNSES GSVVLNDTSK NFEDSRKVAK AAWDMDFTDE QIYVLRKICE AVYNRSAALA AGTIAAIAKR IKIIEHSKFT CGVDGSLFVK NAWYCKRLQE HLKVILADKA ENLIIIPADD GSGKGAAITA AVIALNADIP QLP
NT Sequence
atgagtgagt acgatattgc aaaaaatgat gtaacatata ccaagttaga tacaatagaa tgtgatatac caataaatga agagttatca tggagaatta ataaatttgt gaatcagtta agaatatctt attcaacatt agaagaattt gtagacaatt ttgtatatga attaaagaaa ggtttagaag ctcatcgtaa acatccaaat ttatggattc ctcatgagtg tagttttaag atgttggatt catgtattgc taatattcct actggtcaag agaagggtac atattatgct atagatttcg gtggtactaa ttttcgagct gtaagagctt cattagatgg aaaaggaaaa ataaagagag atcaagaaac atatagttta aaatttacag gatcctattc tcatgagaaa ggtttattag ataagcatgc aacagcatca caattatttg atcattttgc tgaaagaatt aaatatatta tgggggaatt taatgattta gataataaag aagtgaagag tgttggattt accttttctt tcccttgtac atcaccttca attaattgtt caattcttat tgattggaca aagggatttg aaacaggtag agctacgaat gatccagtag aaggtcgtga cgtatgtaaa ttaatgaatg atgcttttgt aagagctgct attccagcga aagtatgttg tgtattgaat gatgctgttg gtactcttat gtcatgtgca tatcaaaaag gtagaggtac cccaccatgt tatataggta ttatattagg tactggttct aatggttgtt attatgaacc tgaatggaag aaatataagt atgctggaaa aattataaat atcgaatttg gtaattttga taaagattta cctacatcac ccatcgattt agttatggat tggtattcag ctaatcgtag tagacaattg tttgaaaaaa tgatatctgg tgcttactta ggtgaaatcg taagaagatt tatggtaaat gttttacaaa gtgcatgttc taaaaagatg tggattagtg atagtttcaa ttcagaatct ggtagtgttg tattaaatga tacttcaaaa aattttgaag atagtaggaa agttgctaag gctgcttggg atatggattt tactgatgaa caaatttatg tcttacgtaa aatttgtgaa gctgtatata atagatctgc agctcttgct gctggtacta tagctgctat agctaaaaga atcaaaatta ttgaacattc taaatttact tgtggtgttg atggctcctt atttgttaaa aatgcttggt attgtaaaag attacaagaa catttaaaag ttatcttagc tgacaaagct gaaaatttaa ttattattcc agcagatgat ggttcaggaa agggagcagc catcacagca gcagtcatcg cattaaatgc ggacattcca caattaccat aa
Details for PlfaA.10272.a.B1.GE37853
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 56kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 79
Validated AA Sequence
MAHHHHHHMS EYDIAKNDVT YTKLDTIECD IPINEELSWR INKFVNQLRI SYSTLEEFVD NFVYELKKGL EAHRKHPNLW IPHECSFKML DSCIANIPTG QEKGTYYAID FGGTNFRAVR ASLDGKGKIK RDQETYSLKF TGSYSHEKGL LDKHATASQL FDHFAERIKY IMGEFNDLDN KEVKSVGFTF SFPCTSPSIN CSILIDWTKG FETGRATNDP VEGRDVCKLM NDAFVRAAIP AKVCCVLNDA VGTLMSCAYQ KGRGTPPCYI GIILGTGSNG CYYEPEWKKY KYAGKIINIE FGNFDKDLPT SPIDLVMDWY SANRSRHCLK NDIWCLLXEI VXIYGKCLQS ACXKKMWXXD SFIQXXXXXX XXYXKXXKXX XKXLXXLGXW XXXXXXQX
Validated NT Sequence
annncnctag antaattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tgagtgagta cgatattgca aaaaatgatg taacatatac caagttagat acaatagaat gtgatatacc aataaatgaa gagttatcat ggagaattaa taaatttgtg aatcagttaa gaatatctta ttcaacatta gaagaatttg tagacaattt tgtatatgaa ttaaagaaag gtttagaagc tcatcgtaaa catccaaatt tatggattcc tcatgagtgt agttttaaga tgttggattc atgtattgct aatattccta ctggtcaaga gaagggtaca tattatgcta tagatttcgg tggtactaat tttcgagctg taagagcttc attagatgga aaaggaaaaa taaagagaga tcaagaaaca tatagtttaa aatttacagg atcctattct catgagaaag gtttattaga taagcatgca acagcatcac aattatttga tcattttgct gaaagaatta aatatattat gggggaattt aatgatttag ataataaaga agtgaagagt gttggattta ccttttcttt cccttgtaca tcaccttcaa ttaattgttc aattcttatt gattggacaa agggatttga aacaggtaga gctacgaatg atccagtaga aggtcgtgac gtatgtaaat taatgaatga tgcttttgta agagctgcta ttccagcgaa agtatgttgt gtattgaatg atgctgttgg tactcttatg tcatgtgcat atcaaaaagg tagaggtacc ccaccatgtt atataggtat tatattaggt actggttcta atggttgtta ttatgaacct gaatggaaga aatataagta tgctggaaaa attataaata tcgaatttgg taattttgat aaagatttac ctacatcacc catcgattta gttatggatt ggtattcagc taatcgtagt agacattgtt tgaaaaatga tatctggtgc ttacttantg aaatcgtaga natttatggt aaatgtttac aaagtgcatg tnctaaaaag atgtggntta nngatagttt cattcagann ntggnnnntt nnnnnnnnnn atactnnaaa nnttnnaagn annnnnnnaa gttnctaagn nnnttgggga nntggatntn nnntgnnnng aancaannnn g
Expected Protein Sequence
MAHHHHHHMS EYDIAKNDVT YTKLDTIECD IPINEELSWR INKFVNQLRI SYSTLEEFVD NFVYELKKGL EAHRKHPNLW IPHECSFKML DSCIANIPTG QEKGTYYAID FGGTNFRAVR ASLDGKGKIK RDQETYSLKF TGSYSHEKGL LDKHATASQL FDHFAERIKY IMGEFNDLDN KEVKSVGFTF SFPCTSPSIN CSILIDWTKG FETGRATNDP VEGRDVCKLM NDAFVRAAIP AKVCCVLNDA VGTLMSCAYQ KGRGTPPCYI GIILGTGSNG CYYEPEWKKY KYAGKIINIE FGNFDKDLPT SPIDLVMDWY SANRSRQLFE KMISGAYLGE IVRRFMVNVL QSACSKKMWI SDSFNSESGS VVLNDTSKNF EDSRKVAKAA WDMDFTDEQI YVLRKICEAV YNRSAALAAG TIAAIAKRIK IIEHSKFTCG VDGSLFVKNA WYCKRLQEHL KVILADKAEN LIIIPADDGS GKGAAITAAV IALNADIPQL P
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg agtacgatat tgcaaaaaat gatgtaacat ataccaagtt agatacaata gaatgtgata taccaataaa tgaagagtta tcatggagaa ttaataaatt tgtgaatcag ttaagaatat cttattcaac attagaagaa tttgtagaca attttgtata tgaattaaag aaaggtttag aagctcatcg taaacatcca aatttatgga ttcctcatga gtgtagtttt aagatgttgg attcatgtat tgctaatatt cctactggtc aagagaaggg tacatattat gctatagatt tcggtggtac taattttcga gctgtaagag cttcattaga tggaaaagga aaaataaaga gagatcaaga aacatatagt ttaaaattta caggatccta ttctcatgag aaaggtttat tagataagca tgcaacagca tcacaattat ttgatcattt tgctgaaaga attaaatata ttatggggga atttaatgat ttagataata aagaagtgaa gagtgttgga tttacctttt ctttcccttg tacatcacct tcaattaatt gttcaattct tattgattgg acaaagggat ttgaaacagg tagagctacg aatgatccag tagaaggtcg tgacgtatgt aaattaatga atgatgcttt tgtaagagct gctattccag cgaaagtatg ttgtgtattg aatgatgctg ttggtactct tatgtcatgt gcatatcaaa aaggtagagg taccccacca tgttatatag gtattatatt aggtactggt tctaatggtt gttattatga acctgaatgg aagaaatata agtatgctgg aaaaattata aatatcgaat ttggtaattt tgataaagat ttacctacat cacccatcga tttagttatg gattggtatt cagctaatcg tagtagacaa ttgtttgaaa aaatgatatc tggtgcttac ttaggtgaaa tcgtaagaag atttatggta aatgttttac aaagtgcatg ttctaaaaag atgtggatta gtgatagttt caattcagaa tctggtagtg ttgtattaaa tgatacttca aaaaattttg aagatagtag gaaagttgct aaggctgctt gggatatgga ttttactgat gaacaaattt atgtcttacg taaaatttgt gaagctgtat ataatagatc tgcagctctt gctgctggta ctatagctgc tatagctaaa agaatcaaaa ttattgaaca ttctaaattt acttgtggtg ttgatggctc cttatttgtt aaaaatgctt ggtattgtaa aagattacaa gaacatttaa aagttatctt agctgacaaa gctgaaaatt taattattat tccagcagat gatggttcag gaaagggagc agccatcaca gcagcagtca tcgcattaaa tgcggacatt ccacaattac catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for PlfaA.10272.a.B11.GE38824
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS EYDIAKNDVT YTKLDTIECD IPINEELSWR INKFVNQLRI SYSTLEEFVD NFVYELKKGL EAHRKHPNLW IPHECSFKML DSCIANIPTG QEKGTYYAID FGGTNFRAVR ASLDGKGKIK RDQETYSLKF TGSYSHEKGL LDKHATASQL FDHFAERIKY IMGEFNDLDN KEVKSVGFTF SFPCTSPSIN CSILIDWTKG FETGRATNDP VEGRDVCKLM NDAFVRAAIP AKVCCVLNDA VGTLMSCAYQ KGRGTPPCYI GIILGTGSNG CYYEPEWKKY KYAGKIINIE FGNFDKDLPT SPIDLVMDWY SANRSRQLFE KMISGAYLGE IVRRFMVNVL QSACSKKMWI SDSFNSESGS VVLNDTSKNF EDSRKVAKAA WDMDFTDEQI YVLRKICEAV YNRSAALAAG TIAAIAKRIK IIEHSKFTCG VDGSLFVKNA WYCKRLQEHL KVILADKAEN LIIIPADDGS GKGAAITAAV IALNADIPQL P
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg agtacgatat tgcaaaaaat gatgtaacat ataccaagtt agatacaata gaatgtgata taccaataaa tgaagagtta tcatggagaa ttaataaatt tgtgaatcag ttaagaatat cttattcaac attagaagaa tttgtagaca attttgtata tgaattaaag aaaggtttag aagctcatcg taaacatcca aatttatgga ttcctcatga gtgtagtttt aagatgttgg attcatgtat tgctaatatt cctactggtc aagagaaggg tacatattat gctatagatt tcggtggtac taattttcga gctgtaagag cttcattaga tggaaaagga aaaataaaga gagatcaaga aacatatagt ttaaaattta caggatccta ttctcatgag aaaggtttat tagataagca tgcaacagca tcacaattat ttgatcattt tgctgaaaga attaaatata ttatggggga atttaatgat ttagataata aagaagtgaa gagtgttgga tttacctttt ctttcccttg tacatcacct tcaattaatt gttcaattct tattgattgg acaaagggat ttgaaacagg tagagctacg aatgatccag tagaaggtcg tgacgtatgt aaattaatga atgatgcttt tgtaagagct gctattccag cgaaagtatg ttgtgtattg aatgatgctg ttggtactct tatgtcatgt gcatatcaaa aaggtagagg taccccacca tgttatatag gtattatatt aggtactggt tctaatggtt gttattatga acctgaatgg aagaaatata agtatgctgg aaaaattata aatatcgaat ttggtaattt tgataaagat ttacctacat cacccatcga tttagttatg gattggtatt cagctaatcg tagtagacaa ttgtttgaaa aaatgatatc tggtgcttac ttaggtgaaa tcgtaagaag atttatggta aatgttttac aaagtgcatg ttctaaaaag atgtggatta gtgatagttt caattcagaa tctggtagtg ttgtattaaa tgatacttca aaaaattttg aagatagtag gaaagttgct aaggctgctt gggatatgga ttttactgat gaacaaattt atgtcttacg taaaatttgt gaagctgtat ataatagatc tgcagctctt gctgctggta ctatagctgc tatagctaaa agaatcaaaa ttattgaaca ttctaaattt acttgtggtg ttgatggctc cttatttgtt aaaaatgctt ggtattgtaa aagattacaa gaacatttaa aagttatctt agctgacaaa gctgaaaatt taattattat tccagcagat gatggttcag gaaagggagc agccatcaca gcagcagtca tcgcattaaa tgcggacatt ccacaattac catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat