SSGCID: Target Page

PlfaA.10201.a

Methionine--tRNA ligase (MetRS) (MRS)

CENTER ID:	PlfaA.10201.a
ORGANISM:	Plasmodium falciparum 3D7
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlfaA.10201.a.B2.GE45431	AD domain		222	722

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PF3D7_1034900
UniProt:	Q8IJ60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMKPLNMILY PHKFFLNTVK CMLAAHIYNF NVELSEDFLY TRTFDIEKSL IINKKPLLIY ENNYVSSTKA ICFLFHRLRN KSKKPFDENL SYYLGWLEWS DLLEKHIEVL NKKNIIESLD ELELYLKENK NKNKNFINND DEKDENKLSL ADIFVYTSLK HSCIEICKES WVHINEYIEK INNLQEIQNV IKNVEEIYKY KNIYSVFISK IHDKNVNEYL KNETFYITTA INYVNGDPHI GHAYEIVLAD AIARYHKIIG RDTFFTTGAD EHGLKIANQA AKNNMTPKEL CDKNVLKFQN LNKLLYVEDD YYVRTTRDSH KKIAQEVWDK CEKNGDIYLG EYEGWYNVRE ETYVPENEAK LMNYIDPLNN IKLEKMKEPS YFFKMSKYQD KLIEYIQSNP DYIQPEQKRN EILQRLKEPL ADLSCSRTKF TWGIQVPNDP KHVMYVWMDA LINYYSSCFI DEGKKKYWPA NVHIIGKDIT WFHTVIFPTI LLSANIPLSK SVFSHGFVLA ADGKKMSKSL GNVINPLEII DKYGADAFRY HVIKETKRGC DTRFDVDNLV DMCNSDLADT IGNLVQRTLS LCQLCNESKI PAFYPEYEIH LPIDILHFIN RVEYHMKTFC VQKCCEKSVQ VCKDLNKFLT DMAPWKYKDD LKNKKLHIIR IMLEAIYFIA HYLDIFIPSL SHKIFEKLNT PKKVIADLSP WLNNLTEGVI INNDNILFKK YEVETAKIKL QKVIIRVCKI VNIINTESSQ NTTICEIEVD NDKITAVLYL PYNNNNNKLV NLLTLAILNI KPLTINNITV NAIIPHVRKE IFTFPIEERI PTGTLIQGKN YKTLVKQRDN LTKKEVVSLD LSIINNKCFF EKNIPLVFAS HEDKQVYHST QDNGSLTFF

NT Sequence

atgatgaagc ctttaaatat gattttatat cctcacaagt tttttttgaa tacagtaaaa tgtatgcttg ctgctcatat atacaatttt aatgtagaac ttagtgaaga ctttttatat actagaacat ttgatattga gaaaagttta ataattaata agaagccatt attaatatat gagaataact atgtgagttc aactaaggct atatgttttt tatttcatcg tttacgaaat aaaagtaaga aaccatttga tgagaacctt agttattatt taggttggtt agaatggagt gatcttttag agaagcatat agaggtgtta aataagaaga atattataga aagtttagat gaattagaat tgtatttaaa ggaaaataaa aataagaata aaaattttat taacaatgat gatgaaaagg atgagaacaa attaagctta gctgatatat ttgtatatac atcattaaaa cattcgtgta tagaaatatg taaagaaagt tgggttcata taaatgaata tattgaaaag attaataatt tacaagaaat acaaaatgtt ataaagaatg ttgaagagat atataaatat aaaaatatat atagtgtatt tatatctaaa atccatgata agaatgttaa tgaatattta aagaatgaaa cgttttatat aacaacagct attaattatg tgaatggtga tccacatata ggacatgcat atgaaattgt attagcagat gcaattgcaa gatatcataa aattatcgga agagatactt tttttacgac aggagcagat gaacatggac taaaaattgc taaccaggcg gccaaaaaca atatgacacc taaggaatta tgtgataaga atgtattaaa atttcaaaat ttgaataaat tgctatatgt agaggatgat tattacgtta ggaccaccag agattcacat aaaaagattg ctcaagaggt gtgggataaa tgtgaaaaaa atggtgatat atatttaggt gaatatgaag gatggtataa tgtcagagaa gaaacgtatg tacccgaaaa tgaagcaaaa ttaatgaatt atattgatcc tttaaataat ataaaattag aaaaaatgaa agaaccatct tattttttta aaatgtcaaa atatcaggat aaattaatag aatatataca atctaatcct gattatattc aacctgaaca aaaaagaaat gaaatcttac aaaggcttaa ggaaccttta gctgatttat catgtagtcg tacaaaattt acatggggta tccaagtacc aaatgatcca aaacatgtaa tgtatgtgtg gatggatgca ttaataaatt attattcaag ttgttttatt gatgagggaa agaaaaaata ttggccagca aatgtacata ttataggtaa ggatataaca tggtttcata cagttatatt tccaaccata ttattatcag ctaatatacc cttatcaaaa agtgtttttt ctcatggatt tgtactagct gcagatggaa agaaaatgtc caaatcttta ggaaatgtaa taaatccatt agaaattatt gataaatatg gagcagatgc atttagatat catgttatta aagaaactaa aagaggatgt gacacaagat ttgatgttga taatttagta gatatgtgta attctgattt agctgataca ataggaaatt tagttcaaag aactttatct ctgtgtcaat tatgtaatga atctaaaata cctgcatttt atccagagta cgaaatacat ttacctattg atattttaca tttcattaat agagtagaat atcatatgaa gactttttgt gttcaaaaat gttgtgagaa aagtgttcag gtttgtaaag atttaaataa attcttaaca gatatggcac catggaaata taaagatgat ttgaaaaata aaaagttaca tattattagg ataatgttag aagcaatata ttttattgcg cattatttgg atatttttat accatcttta tctcataaaa tttttgaaaa attgaataca cccaaaaaag tcattgcaga tttgtcccct tggcttaaca atttaactga aggagttata ataaataatg ataatatatt atttaaaaaa tatgaagtag aaacagccaa aattaagttg caaaaggtta ttataagggt atgtaaaatt gttaatataa ttaacactga gtcgtcacaa aatacaacca tttgtgaaat agaggtagat aatgataaaa taacagctgt tctatatcta ccatataata ataataataa caaacttgtt aatttattaa ctcttgcaat attgaatata aaacccctta ccattaataa tataaccgtt aatgcaatca ttccacatgt acgtaaagaa atatttacat ttcccataga agaaagaatt ccaacaggaa ctttaattca aggaaaaaat tataaaacac ttgttaagca aagagataat ttaaccaaaa aagaggtagt ctcattggat ctatctatta taaataataa gtgttttttt gagaagaata ttcctttagt ttttgcttct catgaagata agcaggttta tcactcaacc caagacaatg gttcacttac ctttttttaa

Details for PlfaA.10201.a.B2.GE45431

HARVESTED ON:	4/17/2024
SEQUENCED ON:	5/15/2024
EXPECTED MW:	60kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHNE TFYITTAINY VNGDPHIGHA YEIVLADAIA RYHKIIGRDT FFTTGADEHG LKIANQAAKN NMTPKELCDK NVLKFQNLNK LLYVEDDYYV RTTRDSHKKI AQEVWDKCEK NGDIYLGEYE GWYNVREETY VPENEAKLMN YIDPLNNIKL EKMKEPSYFF KMSKYQDKLI EYIQSNPDYI QPEQKRNEIL QRLKEPLADL SCSRTKFTWG IQVPNDPKHV MYVWMDALIN YYSSCFIDEG KKKYWPANVH IIGKDITWFH TVIFPTILLS ANIPLSKSVF SHGFVLAADG KKMSKSLGNV INPLEIIDKY GADAFRYHVI KETKRGCDTR FDVDNLVDMC NSDLADTIGN LVQRTLSLCQ LCNESKIPAF YPEYEIHLPI DILHFINRVE YHMKTFCVQK CCEKSVQVCK DLNKFLTDMA PWKYKDDLKN KKLHIIRIML EAIYFIAHYL DIFIPSLSHK IFEKLNTPKK VIADLSPWLN NLTEGVIINN DNILFKKYE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatgaaa cgttttatat aacaacagct attaattatg tgaatggtga tccacatata ggacatgcat atgaaattgt attagcagat gcaattgcaa gatatcataa aattatcgga agagatactt tttttacgac aggagcagat gaacatggac taaaaattgc taaccaggcg gccaaaaaca atatgacacc taaggaatta tgtgataaga atgtattaaa atttcaaaat ttgaataaat tgctatatgt agaggatgat tattacgtta ggaccaccag agattcacat aaaaagattg ctcaagaggt gtgggataaa tgtgaaaaaa atggtgatat atatttaggt gaatatgaag gatggtataa tgtcagagaa gaaacgtatg tacccgaaaa tgaagcaaaa ttaatgaatt atattgatcc tttaaataat ataaaattag aaaaaatgaa agaaccatct tattttttta aaatgtcaaa atatcaggat aaattaatag aatatataca atctaatcct gattatattc aacctgaaca aaaaagaaat gaaatcttac aaaggcttaa ggaaccttta gctgatttat catgtagtcg tacaaaattt acatggggta tccaagtacc aaatgatcca aaacatgtaa tgtatgtgtg gatggatgca ttaataaatt attattcaag ttgttttatt gatgagggaa agaaaaaata ttggccagca aatgtacata ttataggtaa ggatataaca tggtttcata cagttatatt tccaaccata ttattatcag ctaatatacc cttatcaaaa agtgtttttt ctcatggatt tgtactagct gcagatggaa agaaaatgtc caaatcttta ggaaatgtaa taaatccatt agaaattatt gataaatatg gagcagatgc atttagatat catgttatta aagaaactaa aagaggatgt gacacaagat ttgatgttga taatttagta gatatgtgta attctgattt agctgataca ataggaaatt tagttcaaag aactttatct ctgtgtcaat tatgtaatga atctaaaata cctgcatttt atccagagta cgaaatacat ttacctattg atattttaca tttcattaat agagtagaat atcatatgaa gactttttgt gttcaaaaat gttgtgagaa aagtgttcag gtttgtaaag atttaaataa attcttaaca gatatggcac catggaaata taaagatgat ttgaaaaata aaaagttaca tattattagg ataatgttag aagcaatata ttttattgcg cattatttgg atatttttat accatcttta tctcataaaa tttttgaaaa attgaataca cccaaaaaag tcattgcaga tttgtcccct tggcttaaca atttaactga aggagttata ataaataatg ataatatatt atttaaaaaa tatgaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t