PlfaA.01586.a

ATP-dependent RNA Helicase, putative (EC 3.6.1.3)

CENTER ID: PlfaA.01586.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.01586.a.B10.GE36223 Crowther HHPred domain 2, longer 266 433
PlfaA.01586.a.B3.GE36224 Crowther HHPred domain 1 34 265
PlfaA.01586.a.B5.GE36226 Crowther HHPred domains 1 & 2, longer 34 433
PlfaA.01586.a.B6.GE36227 Crowther ModBase, HHPred domain 1 64 265
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.01586.a.B3.PS01776 Crowther HHPred domain 1 34 265

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_0320800
RefSeq: XP_001351278.1
UniProt: O97285

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSYKTNCTNS NANTNTLNSS SNYNKIDDNI ILDEEWKKKI LEPLKDLRYK TEDVTKTKGN EFEDYFLKRE LLMGIFEKGY EKPSPIQEES IPVALAGKNI LARAKNGTGK TAAFAIPLLE KCNTHKNFIQ GLILVPTREL ALQTSAMIKE LGKHMKVQCM VTTGGTSLRE DIMRLYNVVH ILCGTPGRIL DLANKDVANL SGCHIMVMDE ADKLLSPEFQ PIVEELMKFL PKEKQILMYS ATFPVTVKEF RAIYLSDAHE INLMDELTLK GITQYYAFVK ERQKVHCLNT LFAKLQINQA IIFCNSITRV ELLAKKITEL GYSSFYIHAR MSQTHRNRVF HDFRNGACRC LVSSDLFTRG IDIQSVNVVI NFDFPKNSET YLHRIGRSGR YGHLGLAINL ITFEDRFNLY KIEVELGTEI QPIPNEIDPS LYT
NT Sequence
atgagttata aaaccaattg tacgaactct aatgctaata caaatacttt gaatagttct tcaaattata acaaaataga tgataatata atattagatg aagaatggaa aaagaaaatt ctggaaccat taaaagatct aagatataag acagaagatg taacgaaaac gaaaggcaat gaatttgaag attatttttt gaagagagaa ttattaatgg gtatctttga aaaaggatat gagaaaccat cacctataca agaggaaagt atacctgtag ctttggctgg aaaaaatatt ttagcaaggg caaaaaatgg tacaggcaaa acagcagctt ttgctatacc cttactagag aaatgtaata cccacaaaaa ttttattcaa ggactcattt tggtacccac gcgagaactt gccctacaga cctctgctat gattaaggaa ttaggaaaac acatgaaagt acagtgtatg gtaacaaccg gtggtacatc attaagagaa gatataatga ggttgtataa tgtagttcat attttatgtg gtactccagg aagaatatta gacttagcaa ataaggatgt agcaaattta tcaggttgtc atattatggt tatggatgaa gcagataaat tattatcacc tgaatttcaa cctatagtag aagaactaat gaaattttta ccaaaagaaa agcagatact tatgtattct gctacctttc ctgtgactgt aaaagaattt cgagctattt atttatcaga tgcccatgaa ataaatctta tggatgaatt aaccttaaaa ggaataacac aatattatgc ttttgttaaa gaaagacaaa aagtacattg tttaaataca ttatttgcta aacttcaaat taatcaagct atcatcttct gtaatagtat tactagggta gaactactag ccaaaaaaat taccgaacta ggatatagct ctttttacat tcatgcaaga atgtcacaaa cacatcgtaa tcgtgttttt catgatttta gaaatggagc atgcagatgt ttagtttcat cagatctatt cacaagaggt atcgacatac agtcagtcaa tgttgttatc aattttgatt tcccaaagaa ttctgaaact tatttacata gaataggaag atcaggaaga tacggacatc taggactagc tattaatctt ataacttttg aagatcgttt taatttatat aaaatagaag tagaactagg aacggaaata caaccaatac caaacgaaat tgacccatcc ttatatacct aa
Details for PlfaA.01586.a.B10.GE36223
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 21kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME LTLKGITQYY AFVKERQKVH CLNTLFAKLQ INQAIIFCNS ITRVELLAKK ITELGYSSFY IHARMSQTHR NRVFHDFRNG ACRCLVSSDL FTRGIDIQSV NVVINFDFPK NSETYLHRIG RSGRYGHLGL AINLITFEDR FNLYKIEVEL GTEIQPIPNE IDPSLYT
Validated NT Sequence
atcttactca cttaggtata taaggatggg tcaatttcgt ttggtattgg ttgtatttcc gttcctagtt ctacttctat tttatataaa ttaaaacgat cttcaaaagt tataagatta atagctagtc ctagatgtcc gtatcttcct gatcttccta ttctatgtaa ataagtttca gaattctttg ggaaatcaaa attgataaca acattgactg actgtatgtc gatacctctt gtgaatagat ctgatgaaac taaacatctg catgctccat ttctaaaatc atgaaaaaca cgattacgat gtgtttgtga cattcttgca tgaatgtaaa aagagctata tcctagttcg gtaatttttt tggctagtag ttctacccta gtaatactat tacagaagat gatagcttga ttaatttgaa gtttagcaaa taatgtattt aaacaatgta ctttttgtct ttctttaaca aaagcataat attgtgttat tccttttaag gttaattcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHME LTLKGITQYY AFVKERQKVH CLNTLFAKLQ INQAIIFCNS ITRVELLAKK ITELGYSSFY IHARMSQTHR NRVFHDFRNG ACRCLVSSDL FTRGIDIQSV NVVINFDFPK NSETYLHRIG RSGRYGHLGL AINLITFEDR FNLYKIEVEL GTEIQPIPNE IDPSLYT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat taaccttaaa aggaataaca caatattatg cttttgttaa agaaagacaa aaagtacatt gtttaaatac attatttgct aaacttcaaa ttaatcaagc tatcatcttc tgtaatagta ttactagggt agaactacta gccaaaaaaa ttaccgaact aggatatagc tctttttaca ttcatgcaag aatgtcacaa acacatcgta atcgtgtttt tcatgatttt agaaatggag catgcagatg tttagtttca tcagatctat tcacaagagg tatcgacata cagtcagtca atgttgttat caattttgat ttcccaaaga attctgaaac ttatttacat agaataggaa gatcaggaag atacggacat ctaggactag ctattaatct tataactttt gaagatcgtt ttaatttata taaaatagaa gtagaactag gaacggaaat acaaccaata ccaaacgaaa ttgacccatc cttatatacc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for PlfaA.01586.a.B3.GE36224
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME EWKKKILEPL KDLRYKTEDV TKTKGNEFED YFLKRELLMG IFEKGYEKPS PIQEESIPVA LAGKNILARA KNGTGKTAAF AIPLLEKCNT HKNFIQGLIL VPTRELALQT SAMIKELGKH MKVQCMVTTG GTSLREDIMR LYNVVHILCG TPGRILDLAN KDVANLSGCH IMVMDEADKL LSPEFQPIVE ELMKFLPKEK QILMYSATFP VTVKEFRAIY LSDAHEINLM D
Validated NT Sequence
atcttactca cttaatccat aagatttatt tcatgggcat ctgataaata aatagctcga aattctttta cagtcacagg aaaggtagca gaatacataa gtatctgctt ttcttttggt aaaaatttca ttagttcttc tactataggt tgaaattcag gtgataataa tttatctgct tcatccataa ccataatatg acaacctgat aaatttgcta catccttatt tgctaagtct aatattcttc ctggagtacc acataaaata tgaactacat tatacaacct cattatatct tctcttaatg atgtaccacc ggttgttacc atacactgta ctttcatgtg ttttcctaat tccttaatca tagcagaggt ctgtagggca agttctcgcg tgggtaccaa aatgagtcct tgaataaaat ttttgtgggt attacatttc tctagtaagg gtatagcaaa agctgctgtt ttgcctgtac cattttttgc ccttgctaaa atattttttc cagccaaagc tacaggtata ctttcctctt gtataggtga tggtttctca tatccttttt caaagatacc cattaataat tctctcttca aaaaataatc ttcaaattca ttgcctttcg ttttcgttac atcttctgtc ttatatctta gatcttttaa tggttccaga attttctttt tccattcttc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHME EWKKKILEPL KDLRYKTEDV TKTKGNEFED YFLKRELLMG IFEKGYEKPS PIQEESIPVA LAGKNILARA KNGTGKTAAF AIPLLEKCNT HKNFIQGLIL VPTRELALQT SAMIKELGKH MKVQCMVTTG GTSLREDIMR LYNVVHILCG TPGRILDLAN KDVANLSGCH IMVMDEADKL LSPEFQPIVE ELMKFLPKEK QILMYSATFP VTVKEFRAIY LSDAHEINLM D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag aatggaaaaa gaaaattctg gaaccattaa aagatctaag atataagaca gaagatgtaa cgaaaacgaa aggcaatgaa tttgaagatt attttttgaa gagagaatta ttaatgggta tctttgaaaa aggatatgag aaaccatcac ctatacaaga ggaaagtata cctgtagctt tggctggaaa aaatatttta gcaagggcaa aaaatggtac aggcaaaaca gcagcttttg ctataccctt actagagaaa tgtaataccc acaaaaattt tattcaagga ctcattttgg tacccacgcg agaacttgcc ctacagacct ctgctatgat taaggaatta ggaaaacaca tgaaagtaca gtgtatggta acaaccggtg gtacatcatt aagagaagat ataatgaggt tgtataatgt agttcatatt ttatgtggta ctccaggaag aatattagac ttagcaaata aggatgtagc aaatttatca ggttgtcata ttatggttat ggatgaagca gataaattat tatcacctga atttcaacct atagtagaag aactaatgaa atttttacca aaagaaaagc agatacttat gtattctgct acctttcctg tgactgtaaa agaatttcga gctatttatt tatcagatgc ccatgaaata aatcttatgg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for PlfaA.01586.a.B5.GE36226
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 47kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHME EWKKKILEPL KDLRYKTEDV TKTKGNEFED YFLKRELLMG IFEKGYEKPS PIQEESIPVA LAGKNILARA KNGTGKTAAF AIPLLEKCNT HKNFIQGLIL VPTRELALQT SAMIKELGKH MKVQCMVTTG GTSLREDIMR LYNVVHILCG TPGRILDLAN KDVANLSGCH IMVMDEADKL LSPEFQPIVE ELMKFLPKEK QILMYSATFP VTVKEFRAIY LSDAHEINLM DELTLKGITQ YYAFVKERQK VHCLNTLFAK LQINQAIIFC NSITRVELLA KKITELXYSS FYIHARMSQT HXNRVFHDFR NGACXCLVSS DLFXXXRXTV SQCXXXXXXX XXLXLXXXXX XSXXXLXXXX XXXXXXXXXX XXXXXXXXXX XPXXXXX
Validated NT Sequence
ttttgnnnnn tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag aatggaaaaa gaaaattctg gaaccattaa aagatctaag atataagaca gaagatgtaa cgaaaacgaa aggcaatgaa tttgaagatt attttttgaa gagagaatta ttaatgggta tctttgaaaa aggatatgag aaaccatcac ctatacaaga ggaaagtata cctgtagctt tggctggaaa aaatatttta gcaagggcaa aaaatggtac aggcaaaaca gcagcttttg ctataccctt actagagaaa tgtaataccc acaaaaattt tattcaagga ctcattttgg tacccacgcg agaacttgcc ctacagacct ctgctatgat taaggaatta ggaaaacaca tgaaagtaca gtgtatggta acaaccggtg gtacatcatt aagagaagat ataatgaggt tgtataatgt agttcatatt ttatgtggta ctccaggaag aatattagac ttagcaaata aggatgtagc aaatttatca ggttgtcata ttatggttat ggatgaagca gataaattat tatcacctga atttcaacct atagtagaag aactaatgaa atttttacca aaagaaaagc agatacttat gtattctgct acctttcctg tgactgtaaa agaatttcga gctatttatt tatcagatgc ccatgaaata aatcttatgg atgaattaac cttaaaagga ataacacaat attatgcttt tgttaaagaa agacaaaaag tacattgttt aaatacatta tttgctaaac ttcaaattaa tcaagctatc atcttctgta atagtattac tagggtagaa ctactagcca aaaaaattac cgaactanga tatagctctt tttacattca tgcnagaatg tcacaaacac atcnnaatcg tgtttttcat gattttagaa atggagcatg canatgttta gtttcntcag atctattcnn annnnatcga cntacagtca gtcaatgtnt nntcantttn nanttncnna nnnnnctgaa ncttnnttnn nnnnaannnn nntcnnnnga ngnactagnt nnnnnnntnn nnnttnnnnn nnnnnttnnn tnnnnnnann annnnnntnn nnnnnnnnnn nnnnnnnnnn nnccnnncnn nnnncnnnnn na
Expected Protein Sequence
MAHHHHHHME EWKKKILEPL KDLRYKTEDV TKTKGNEFED YFLKRELLMG IFEKGYEKPS PIQEESIPVA LAGKNILARA KNGTGKTAAF AIPLLEKCNT HKNFIQGLIL VPTRELALQT SAMIKELGKH MKVQCMVTTG GTSLREDIMR LYNVVHILCG TPGRILDLAN KDVANLSGCH IMVMDEADKL LSPEFQPIVE ELMKFLPKEK QILMYSATFP VTVKEFRAIY LSDAHEINLM DELTLKGITQ YYAFVKERQK VHCLNTLFAK LQINQAIIFC NSITRVELLA KKITELGYSS FYIHARMSQT HRNRVFHDFR NGACRCLVSS DLFTRGIDIQ SVNVVINFDF PKNSETYLHR IGRSGRYGHL GLAINLITFE DRFNLYKIEV ELGTEIQPIP NEIDPSLYT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag aatggaaaaa gaaaattctg gaaccattaa aagatctaag atataagaca gaagatgtaa cgaaaacgaa aggcaatgaa tttgaagatt attttttgaa gagagaatta ttaatgggta tctttgaaaa aggatatgag aaaccatcac ctatacaaga ggaaagtata cctgtagctt tggctggaaa aaatatttta gcaagggcaa aaaatggtac aggcaaaaca gcagcttttg ctataccctt actagagaaa tgtaataccc acaaaaattt tattcaagga ctcattttgg tacccacgcg agaacttgcc ctacagacct ctgctatgat taaggaatta ggaaaacaca tgaaagtaca gtgtatggta acaaccggtg gtacatcatt aagagaagat ataatgaggt tgtataatgt agttcatatt ttatgtggta ctccaggaag aatattagac ttagcaaata aggatgtagc aaatttatca ggttgtcata ttatggttat ggatgaagca gataaattat tatcacctga atttcaacct atagtagaag aactaatgaa atttttacca aaagaaaagc agatacttat gtattctgct acctttcctg tgactgtaaa agaatttcga gctatttatt tatcagatgc ccatgaaata aatcttatgg atgaattaac cttaaaagga ataacacaat attatgcttt tgttaaagaa agacaaaaag tacattgttt aaatacatta tttgctaaac ttcaaattaa tcaagctatc atcttctgta atagtattac tagggtagaa ctactagcca aaaaaattac cgaactagga tatagctctt tttacattca tgcaagaatg tcacaaacac atcgtaatcg tgtttttcat gattttagaa atggagcatg cagatgttta gtttcatcag atctattcac aagaggtatc gacatacagt cagtcaatgt tgttatcaat tttgatttcc caaagaattc tgaaacttat ttacatagaa taggaagatc aggaagatac ggacatctag gactagctat taatcttata acttttgaag atcgttttaa tttatataaa atagaagtag aactaggaac ggaaatacaa ccaataccaa acgaaattga cccatcctta tatacctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PlfaA.01586.a.B6.GE36227
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD YFLKRELLMG IFEKGYEKPS PIQEESIPVA LAGKNILARA KNGTGKTAAF AIPLLEKCNT HKNFIQGLIL VPTRELALQT SAMIKELGKH MKVQCMVTTG GTSLREDIMR LYNVVHILCG TPGRILDLAN KDVANLSGCH IMVMDEADKL LSPEFQPIVE ELMKFLPKEK QILMYSATFP VTVKEFRAIY LSDAHEINLM D
Validated NT Sequence
tnnnnnnana ttttntttan ctttaagaag gaganatacc atggctcacc accaccacca ccatatggat tattttttga agagagaatt attaatgggt atctttgaaa aaggatatga gaaaccatca cctatacaag aggaaagtat acctgtagct ttggctggaa aaaatatttt agcaagggca aaaaatggta caggcaaaac agcagctttt gctataccct tactagagaa atgtaatacc cacaaaaatt ttattcaagg actcattttg gtacccacgc gagaacttgc cctacagacc tctgctatga ttaaggaatt aggaaaacac atgaaagtac agtgtatggt aacaaccggt ggtacatcat taagagaaga tataatgagg ttgtataatg tagttcatat tttatgtggt actccaggaa gaatattaga cttagcaaat aaggatgtag caaatttatc aggttgtcat attatggtta tggatgaagc agataaatta ttatcacctg aatttcaacc tatagtagaa gaactaatga aatttttacc aaaagaaaag cagatactta tgtattctgc tacctttcct gtgactgtaa aagaatttcg agctatttat ttatcagatg cccatgaaat aaatcttatg gattaagtga gtaagatagg atccggctgn nnnnnncccn nnnnnnnann aantnnnnnn nnnntnccnn nnnctnnncc ccccnnnnnn nnncnnc
Expected Protein Sequence
MAHHHHHHMD YFLKRELLMG IFEKGYEKPS PIQEESIPVA LAGKNILARA KNGTGKTAAF AIPLLEKCNT HKNFIQGLIL VPTRELALQT SAMIKELGKH MKVQCMVTTG GTSLREDIMR LYNVVHILCG TPGRILDLAN KDVANLSGCH IMVMDEADKL LSPEFQPIVE ELMKFLPKEK QILMYSATFP VTVKEFRAIY LSDAHEINLM D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt attttttgaa gagagaatta ttaatgggta tctttgaaaa aggatatgag aaaccatcac ctatacaaga ggaaagtata cctgtagctt tggctggaaa aaatatttta gcaagggcaa aaaatggtac aggcaaaaca gcagcttttg ctataccctt actagagaaa tgtaataccc acaaaaattt tattcaagga ctcattttgg tacccacgcg agaacttgcc ctacagacct ctgctatgat taaggaatta ggaaaacaca tgaaagtaca gtgtatggta acaaccggtg gtacatcatt aagagaagat ataatgaggt tgtataatgt agttcatatt ttatgtggta ctccaggaag aatattagac ttagcaaata aggatgtagc aaatttatca ggttgtcata ttatggttat ggatgaagca gataaattat tatcacctga atttcaacct atagtagaag aactaatgaa atttttacca aaagaaaagc agatacttat gtattctgct acctttcctg tgactgtaaa agaatttcga gctatttatt tatcagatgc ccatgaaata aatcttatgg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for PlfaA.01586.a.B3.PS01776
PURIFICATION DATe: 3/26/2013
CONCENTRATION: 18.11mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME EWKKKILEPL KDLRYKTEDV TKTKGNEFED YFLKRELLMG IFEKGYEKPS PIQEESIPVA LAGKNILARA KNGTGKTAAF AIPLLEKCNT HKNFIQGLIL VPTRELALQT SAMIKELGKH MKVQCMVTTG GTSLREDIMR LYNVVHILCG TPGRILDLAN KDVANLSGCH IMVMDEADKL LSPEFQPIVE ELMKFLPKEK QILMYSATFP VTVKEFRAIY LSDAHEINLM D
Validated NT Sequence
atcttactca cttaatccat aagatttatt tcatgggcat ctgataaata aatagctcga aattctttta cagtcacagg aaaggtagca gaatacataa gtatctgctt ttcttttggt aaaaatttca ttagttcttc tactataggt tgaaattcag gtgataataa tttatctgct tcatccataa ccataatatg acaacctgat aaatttgcta catccttatt tgctaagtct aatattcttc ctggagtacc acataaaata tgaactacat tatacaacct cattatatct tctcttaatg atgtaccacc ggttgttacc atacactgta ctttcatgtg ttttcctaat tccttaatca tagcagaggt ctgtagggca agttctcgcg tgggtaccaa aatgagtcct tgaataaaat ttttgtgggt attacatttc tctagtaagg gtatagcaaa agctgctgtt ttgcctgtac cattttttgc ccttgctaaa atattttttc cagccaaagc tacaggtata ctttcctctt gtataggtga tggtttctca tatccttttt caaagatacc cattaataat tctctcttca aaaaataatc ttcaaattca ttgcctttcg ttttcgttac atcttctgtc ttatatctta gatcttttaa tggttccaga attttctttt tccattcttc catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHME EWKKKILEPL KDLRYKTEDV TKTKGNEFED YFLKRELLMG IFEKGYEKPS PIQEESIPVA LAGKNILARA KNGTGKTAAF AIPLLEKCNT HKNFIQGLIL VPTRELALQT SAMIKELGKH MKVQCMVTTG GTSLREDIMR LYNVVHILCG TPGRILDLAN KDVANLSGCH IMVMDEADKL LSPEFQPIVE ELMKFLPKEK QILMYSATFP VTVKEFRAIY LSDAHEINLM D
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAG AATGGAAAAA GAAAATTCTG GAACCATTAA AAGATCTAAG ATATAAGACA GAAGATGTAA CGAAAACGAA AGGCAATGAA TTTGAAGATT ATTTTTTGAA GAGAGAATTA TTAATGGGTA TCTTTGAAAA AGGATATGAG AAACCATCAC CTATACAAGA GGAAAGTATA CCTGTAGCTT TGGCTGGAAA AAATATTTTA GCAAGGGCAA AAAATGGTAC AGGCAAAACA GCAGCTTTTG CTATACCCTT ACTAGAGAAA TGTAATACCC ACAAAAATTT TATTCAAGGA CTCATTTTGG TACCCACGCG AGAACTTGCC CTACAGACCT CTGCTATGAT TAAGGAATTA GGAAAACACA TGAAAGTACA GTGTATGGTA ACAACCGGTG GTACATCATT AAGAGAAGAT ATAATGAGGT TGTATAATGT AGTTCATATT TTATGTGGTA CTCCAGGAAG AATATTAGAC TTAGCAAATA AGGATGTAGC AAATTTATCA GGTTGTCATA TTATGGTTAT GGATGAAGCA GATAAATTAT TATCACCTGA ATTTCAACCT ATAGTAGAAG AACTAATGAA ATTTTTACCA AAAGAAAAGC AGATACTTAT GTATTCTGCT ACCTTTCCTG TGACTGTAAA AGAATTTCGA GCTATTTATT TATCAGATGC CCATGAAATA AATCTTATGG ATTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT