PlfaA.01559.a

Dephospho-CoA kinase, putative (EC 2.7.1.24) (DPCK)

CENTER ID: PlfaA.01559.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.01559.a.D51.GE00001 Saliba soluble domain 75 274
PlfaA.01559.a.MB51.GE00001 Saliba soluble domain 75 274
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1443700
RefSeq: XP_001348589.1
UniProt: Q8IL34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFLKFFLDKC ILCFLALGSI PIGYINRKNK FRKIENQKYA LFSSIVMNSF LIYLNKFFGI LGIFNFFLGN YLCLIGITGG IAVGKSTFCN FLKKKDVVVI NADEITSKIY TKDSICYKKI VKHFGENILN NDKSINRTLL RKIVFNNEEN VKYINKITHT YIILQIIKEC LKYKFLYFKY NVAIEAPLLI ETKLYLLTSP VILLKSSVRN QIKRILSRDK NCTYDTAMGI IKNQLPTDEK IKYADIIINN DGDILDLQMK CDVVYNKYLK NFFF
NT Sequence
atgtttttaa aattctttct tgataaatgt attttatgtt tcctggcttt gggttcgatt ccaattggat atataaatag aaagaataag tttaggaaaa ttgaaaatca gaaatatgcc ttattcagtt ctatagttat gaacagtttt ttaatatacc taaataagtt ttttggaata ttaggaatat ttaatttttt tttagggaat tatttatgtt taattggaat aacaggtggt atagccgttg gaaaatcaac tttttgtaat tttttaaaaa aaaaagatgt ggttgtaata aatgctgatg aaattactag taagatttat acaaaagatt ctatatgcta taaaaaaatt gtaaaacact ttggagaaaa tattttaaat aatgataaaa gtattaatag aaccttattg agaaaaatag tatttaataa tgaagaaaat gttaaatata tcaataaaat tacacatact tatattatac tacaaattat taaagaatgc ttaaaatata aatttttata ttttaaatat aatgtagcca ttgaagcacc tctattaata gaaacgaaat tatatctatt aacaagtcct gttatacttt taaaatcatc tgtaagaaat caaattaaac gtatcttatc aagagataaa aattgcacct atgatacagc catgggtatt atcaagaatc agctacctac agatgaaaag ataaagtacg cggatattat tattaacaat gatggagata tattagactt acaaatgaaa tgtgatgtgg tatataataa atatttaaaa aactttttct tctaa
Details for PlfaA.01559.a.D51.GE00001
HARVESTED ON: 1/25/2017
SEQUENCED ON: 1/9/2017
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 84
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG SIGITGGIAV GKSTFCNFLK KKDVVVINAD EITSKIYTKD SICYKKIVKH FGENILNNDK SINRTLLRKI VFNNEENVKY INKITHTYII LQIIKECLKY KFLYFKYNVA IEAPLLIETK LYLLTSPVIL LKSSVRNQIK RILSRDKNCT YDTAMGIIKN QLPTDEKIKY ADIIINNDGD ILDLQMKCDV VYNKYLKNFF F
Validated NT Sequence
ttaaaagaaa aagtttttca ggtatttgtt gtacacgacg tcgcatttca tttgcagatc cagaatatcg ccatcgttgt taataataat atcagcatat ttaattttct catcggtagg cagttgattt ttgatgattc ccatcgcggt atcataggtg cagttcttat cgcgagacag aatgcgtttg atctggttac gtacactgga cttcagcaga atcaccggag atgtcagcag gtacagtttg gtttcgatca gcagcggggc ttcaatagca acattatatt tgaagtacag gaatttatat ttcaggcatt ctttaataat ctgcagaatg atatacgtat gggtgatttt gttaatatac ttaacatttt cttcgttatt aaacacaatt ttacgcagca gggtgcgatt gatagatttg tcgttgttca ggatgttttc gccaaaatgt tttacaatct ttttataaca aatagaatct ttcgtgtaga tttttgaagt gatttcatct gcgttaatta ctacaacgtc ttttttcttc agaaagttac aaaaggtgct tttacccact gcaatcccgc ccgtaatacc aatggatcca ccgatttgtt cacggtgtgc ttcgataatg tcgttatctt ccatgtccag atcttccgga gtctggtctg cctgaatacg gataccgtcg tacagaaagc gcagggaatc catttccttg ccctggcgtt tagcgaatgc ttccatcagg cgacgcagcg gggtggtctt cttaatcttg aagaagattt cgctgctacc gtcgctaacc ttcaggttga tgtgggtctc cggcttcact tccggtttta cttcgccgct at
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSIGI TGGIAVGKST FCNFLKKKDV VVINADEITS KIYTKDSICY KKIVKHFGEN ILNNDKSINR TLLRKIVFNN EENVKYINKI THTYIILQII KECLKYKFLY FKYNVAIEAP LLIETKLYLL TSPVILLKSS VRNQIKRILS RDKNCTYDTA MGIIKNQLPT DEKIKYADII INNDGDILDL QMKCDVVYNK YLKNFFF
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggatc cattggtatt acgggcggga ttgcagtggg taaaagcacc ttttgtaact ttctgaagaa aaaagacgtt gtagtaatta acgcagatga aatcacttca aaaatctaca cgaaagattc tatttgttat aaaaagattg taaaacattt tggcgaaaac atcctgaaca acgacaaatc tatcaatcgc accctgctgc gtaaaattgt gtttaataac gaagaaaatg ttaagtatat taacaaaatc acccatacgt atatcattct gcagattatt aaagaatgcc tgaaatataa attcctgtac ttcaaatata atgttgctat tgaagccccg ctgctgatcg aaaccaaact gtacctgctg acatctccgg tgattctgct gaagtccagt gtacgtaacc agatcaaacg cattctgtct cgcgataaga actgcaccta tgataccgcg atgggaatca tcaaaaatca actgcctacc gatgagaaaa ttaaatatgc tgatattatt attaacaacg atggcgatat tctggatctg caaatgaaat gcgacgtcgt gtacaacaaa tacctgaaaa actttttctt taagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
Details for PlfaA.01559.a.MB51.GE00001
HARVESTED ON: 1/25/2017
SEQUENCED ON: 1/9/2017
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 74
Validated AA Sequence
MFNLQEPYFT WPLIAADGGY AFKYENGKYD IKDVGVDNAG AKAGLTFLVD LIKNKHMNAD TDYSIAEAAF NKGETAMTIN GPWAWSNIDT SKVNYGVTVL PTFKGQPSKP FVGVLSAGIN AASPNKELAK EFLENYLLTD EGLEAVNKDK PLGAVALKSY EEELAKDPRI AATMENAQKG EIMPNIPQMS AFWYAVRTAV INAASGRQTV DEALKDAQTN SSSNNNNNNN NNNLGIEGRI SEGTLEAQTQ GPGSRIGITG GIAVGKSTFC NFLKKKDVVV INADEITSKI YTKDSICYKK IVKHFGENIL NNDKSINRTL LRKIVFNNEE NVKYINKITH TYIILQIIKE CLKYKFLYFK YNVAIEAPLL IETKLYLLTS PVILLKSSVR NQIKRILSRD KNCTYDTAMG IIKNQLPTDE KIKYADIIIN NDGDILDLQM KCDVVYNKYL KNFFF
Validated NT Sequence
atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccactatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactaattc gagctcgaac aacaacaaca acaacaataa caacaacctc gggatcgagg gaaggatttc agaaggtacc ctggaagctc agacccaggg tcctggttcg cgaattggta ttacgggcgg gattgcagtg ggtaaaagca ccttttgtaa ctttctgaag aaaaaagacg ttgtagtaat taacgcagat gaaatcactt caaaaatcta cacgaaagat tctatttgtt ataaaaagat tgtaaaacat tttggcgaaa acatcctgaa caacgacaaa tctatcaatc gcaccctgct gcgtaaaatt gtgtttaata acgaagaaaa tgttaagtat attaacaaaa tcacccatac gtatatcatt ctgcagatta ttaaagaatg cctgaaatat aaattcctgt acttcaaata taatgttgct attgaagccc cgctgctgat cgaaaccaaa ctgtacctgc tgacatctcc ggtgattctg ctgaagtcca gtgtacgtaa ccagatcaaa cgcattctgt ctcgcgataa gaactgcacc tatgataccg cgatgggaat catcaaaaat caactgccta ccgatgagaa aattaaatat gctgatatta ttattaacaa cgatggcgat attctggatc tgcaaatgaa atgcgacgtc gtgtacaaca aatacctgaa aaactttttc ttttaagttt a
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS IGITGGIAVG KSTFCNFLKK KDVVVINADE ITSKIYTKDS ICYKKIVKHF GENILNNDKS INRTLLRKIV FNNEENVKYI NKITHTYIIL QIIKECLKYK FLYFKYNVAI EAPLLIETKL YLLTSPVILL KSSVRNQIKR ILSRDKNCTY DTAMGIIKNQ LPTDEKIKYA DIIINNDGDI LDLQMKCDVV YNKYLKNFFF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgattgg tattacgggc gggattgcag tgggtaaaag caccttttgt aactttctga agaaaaaaga cgttgtagta attaacgcag atgaaatcac ttcaaaaatc tacacgaaag attctatttg ttataaaaag attgtaaaac attttggcga aaacatcctg aacaacgaca aatctatcaa tcgcaccctg ctgcgtaaaa ttgtgtttaa taacgaagaa aatgttaagt atattaacaa aatcacccat acgtatatca ttctgcagat tattaaagaa tgcctgaaat ataaattcct gtacttcaaa tataatgttg ctattgaagc cccgctgctg atcgaaacca aactgtacct gctgacatct ccggtgattc tgctgaagtc cagtgtacgt aaccagatca aacgcattct gtctcgcgat aagaactgca cctatgatac cgcgatggga atcatcaaaa atcaactgcc taccgatgag aaaattaaat atgctgatat tattattaac aacgatggcg atattctgga tctgcaaatg aaatgcgacg tcgtgtacaa caaatacctg aaaaactttt tcttttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa