SSGCID: Target Page

PlfaA.01515.a

Aurora kinase (PfArk1)

CENTER ID:	PlfaA.01515.a
ORGANISM:	Plasmodium falciparum 3D7
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlfaA.01515.a.B11.GE36982	full length		31	312

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlfaA.01515.a.HZ3.PW39244	codon-optimized domain #2		7	338

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PF3D7_0605300
RefSeq:	XP_966043.1
UniProt:	C6KSQ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNDSISSPIK ATCRDMALKS SLHSFTFSLK DFDIAGFLGD GAHGSVFLAC ERRTNFICVL KCISKSHLVK STQEALLRKE IELQAHLKHP HIACMYTWFH TSSHVFFVME YCSNGDLFTY LNENGPFCEK KVAEMLFEII WAIRTCHDKR IAHLDLKPEN VLVNHEEKCK LADFGLSAHI GSKHKKKGIS HYRGTHDYWS PEQCARHQKK KQNFGEFDQK TDIWTLGILA FELKFGRPPF GSTNEERENV IMNRIQDYHW SQLFCEKVKQ DLIDKLSPEF KDFLNLCLDK NPKKRPTAES LIQHPFIFIH NKNRSCIKSY ATQPRGKQGP KLSHKGFNEQ DGSFLTPIWF HNK

NT Sequence

atgaacgatt caatcagtag ccccattaag gccacgtgta gagacatggc ccttaaaagt agtttacatt ctttcacctt tagtttgaaa gattttgata tagccggatt tttgggcgat ggagcacatg gtagtgtatt cttggcttgt gagaggagaa ccaattttat atgcgtgtta aaatgtatat ctaaatcaca tttagtcaag agcactcaag aagctttgtt aagaaaagag atagaattgc aggctcattt aaaacaccca catatagcat gcatgtatac atggtttcat acaagcagcc acgtgttttt tgttatggaa tattgctcaa atggagacct ctttacctat ttaaatgaga atggcccatt ttgtgagaaa aaggtagcag agatgctttt tgaaataata tgggctataa gaacatgtca tgataaaaga atagcacatt tggatttgaa acccgaaaat gttttagtaa accatgaaga aaaatgtaag ttagctgatt ttggtttatc agcacatata ggatctaaac ataaaaagaa aggtatatct cattatagag gaacacatga ttattggtct cctgaacaat gtgcaagaca tcaaaagaag aaacagaatt ttggagaatt tgatcagaaa acagatatat ggactctagg gattttagct tttgaattaa aatttggtag acctccattt ggttcaacaa atgaagaaag agaaaatgta attatgaata gaatacaaga ttatcattgg agtcaattat tctgtgaaaa agtaaaacaa gatttaatag ataaattatc acctgaattt aaagatttct taaatttatg tctagataaa aatccaaaaa aaagaccaac tgcagaatcc ttaattcaac atccttttat ttttattcat aataaaaata gatcatgtat caaatcttac gcgacacaac ctaggggtaa acaaggtcca aaattgagcc acaagggatt taatgagcaa gatgggtcct ttctaacacc catatggttt cacaacaaat ga

Details for PlfaA.01515.a.B11.GE36982

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	34kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD FDIAGFLGDG AHGSVFLARE RRTNFICVLK CISKSHLVKS TQEALLRKEI ELQAHLKHPH IACMYTWFHT SSHVFFVMEY CSNGDLFTYL NENGPFAEKK VAEMLFEIIW AIRTCHDKRI AHLDLKPENV LVNHEEKCKL ADFGLSAHIG SKHKKKGISH YRGTHDYWSP EQCARHQKKK QNFGEFDQKT DIWTLGILAF ELKFGRPPFG STNEERENVI MNRIQDYHWS QLFAEKVKQD LIDKLSPEFK DFLNLCLDKN PKKRPTAESL IQHPFIFIHN K

Validated NT Sequence

tcttactcac ttacttgttg tggataaaga taaacgggtg ctggatcagg gattcggcag tcggacgctt cttcggattc ttatcaaggc acaggttcag gaagtctttg aattccgggc tgagcttgtc gatcagatcc tgtttaacct tctcagcgaa cagctgagac cagtggtagt cctggatgcg gttcataata acgttttcgc gttcttcatt agtgctacca aacggaggac gaccgaattt cagttcgaat gcgaggatgc ctaaggtcca gatgtcagtt ttctgatcga attcaccgaa gttctgtttc ttcttctggt gacgagcaca ctgttcagga gaccagtagt cgtgagtgcc acgatagtgg cttatacctt tctttttgtg tttgctaccg atgtgtgcgg aaagaccgaa atctgcgagt ttgcacttct cttcgtggtt gaccagaacg ttttccggtt tcaggtccag gtgagctata cgtttatcgt ggcaggtgcg gatagcccag atgatttcga aaagcatttc ggcaactttt ttttcagcga acgggccgtt ttcgttcagg taagtgaaca gatcgccgtt agagcagtat tccattacga agaaaacgtg agagctagtg tggaaccagg tgtacataca agcgatgtgc gggtgtttca ggtgagcctg cagttcgatt tccttacgca gcagggcttc ctgagtagat ttaactaagt gagatttgga gatgcatttc agtacgcaga tgaagttggt acgacgttca cgtgccagga atacagaacc gtgagcgccg tcgcctaaga agcctgcgat gtcaaagtcc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHDF DIAGFLGDGA HGSVFLARER RTNFICVLKC ISKSHLVKST QEALLRKEIE LQAHLKHPHI ACMYTWFHTS SHVFFVMEYC SNGDLFTYLN ENGPFAEKKV AEMLFEIIWA IRTCHDKRIA HLDLKPENVL VNHEEKCKLA DFGLSAHIGS KHKKKGISHY RGTHDYWSPE QCARHQKKKQ NFGEFDQKTD IWTLGILAFE LKFGRPPFGS TNEERENVIM NRIQDYHWSQ LFAEKVKQDL IDKLSPEFKD FLNLCLDKNP KKRPTAESLI QHPFIFIHNK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgactttg acatcgcagg cttcttaggc gacggcgctc acggttctgt attcctggca cgtgaacgtc gtaccaactt catctgcgta ctgaaatgca tctccaaatc tcacttagtt aaatctactc aggaagccct gctgcgtaag gaaatcgaac tgcaggctca cctgaaacac ccgcacatcg cttgtatgta cacctggttc cacactagct ctcacgtttt cttcgtaatg gaatactgct ctaacggcga tctgttcact tacctgaacg aaaacggccc gttcgctgaa aaaaaagttg ccgaaatgct tttcgaaatc atctgggcta tccgcacctg ccacgataaa cgtatagctc acctggacct gaaaccggaa aacgttctgg tcaaccacga agagaagtgc aaactcgcag atttcggtct ttccgcacac atcggtagca aacacaaaaa gaaaggtata agccactatc gtggcactca cgactactgg tctcctgaac agtgtgctcg tcaccagaag aagaaacaga acttcggtga attcgatcag aaaactgaca tctggacctt aggcatcctc gcattcgaac tgaaattcgg tcgtcctccg tttggtagca ctaatgaaga acgcgaaaac gttattatga accgcatcca ggactaccac tggtctcagc tgttcgctga gaaggttaaa caggatctga tcgacaagct cagcccggaa ttcaaagact tcctgaacct gtgccttgat aagaatccga agaagcgtcc gactgccgaa tccctgatcc agcacccgtt tatctttatc cacaacaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for PlfaA.01515.a.HZ3.PW39244

PURIFICATION DATe:	11/29/2023
CONCENTRATION:	15.3mg/ml
OBSERVED MW:	52kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSSP IKATCRDMAL KSSLHSFTFS LKDFDIAGFL GDGAHGSVFL ACERRTNFIC VLKCISKSHL VKSTQEALLR KEIELQAHLK HPHIACMYTW FHTSSHVFFV MEYCSNGDLF TYLNENGPFC EKKVAEMLFE IIWAIRTCHD KRIAHLDLKP ENVLVNHEEK CKLADFGLSA HIGSKHKKKG ISHYRGTHDY WSPEQCARHQ KKKQNFGEFD QKTDIWTLGI LAFELKFGRP PFGSTNEERE NVIMNRIQDY HWSQLFCEKV KQDLIDKLSP EFKDFLNLCL DKNPKKRPTA ESLIQHPFIF IHNKNRSCIK SYATQPRGKQ GPKLSHKGFN

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGAAGTCGGA CTCAGAAGTC AATCAAGAAG CTAAGCCAGA GGTCAAGCCA GAAGTCAAGC CTGAGACTCA CATCAATTTA AAGGTGTCCG ATGGATCTTC AGAGATCTTC TTCAAGATCA AAAAGACCAC TCCTTTAAGA AGGCTGATGG AAGCGTTCGC TAAAAGACAG GGTAAGGAAA TGGACTCCTT AAGATTCTTG TACGACGGTA TTAGAATTCA AGCTGATCAG ACCCCTGAAG ATTTGGACAT GGAGGATAAC GATATTATTG AGGCTCACAG AGAACAGATT GGTGGTACCC TGGAAGCTCA GACCCAGGGT CCTGGTTCGA GCCCCATTAA GGCCACGTGT AGAGACATGG CCCTTAAAAG TAGTTTACAT TCTTTCACCT TTAGTTTGAA AGATTTTGAT ATAGCCGGAT TTTTGGGCGA TGGAGCACAT GGTAGTGTAT TCTTGGCTTG TGAGAGGAGA ACCAATTTTA TATGCGTGTT AAAATGTATA TCTAAATCAC ATTTAGTCAA GAGCACTCAA GAAGCTTTGT TAAGAAAAGA GATAGAATTG CAGGCTCATT TAAAACACCC ACATATAGCA TGCATGTATA CATGGTTTCA TACAAGCAGC CACGTGTTTT TTGTTATGGA ATATTGCTCA AATGGAGACC TCTTTACCTA TTTAAATGAG AATGGCCCAT TTTGTGAGAA AAAGGTAGCA GAGATGCTTT TTGAAATAAT ATGGGCTATA AGAACATGTC ATGATAAAAG AATAGCACAT TTGGATTTGA AACCCGAAAA TGTTTTAGTA AACCATGAAG AAAAATGTAA GTTAGCTGAT TTTGGTTTAT CAGCACATAT AGGATCTAAA CATAAAAAGA AAGGTATATC TCATTATAGA GGAACACATG ATTATTGGTC TCCTGAACAA TGTGCAAGAC ATCAAAAGAA GAAACAGAAT TTTGGAGAAT TTGATCAGAA AACAGATATA TGGACTCTAG GGATTTTAGC TTTTGAATTA AAATTTGGTA GACCTCCATT TGGTTCAACA AATGAAGAAA GAGAAAATGT AATTATGAAT AGAATACAAG ATTATCATTG GAGTCAATTA TTCTGTGAAA AAGTAAAACA AGATTTAATA GATAAATTAT CACCTGAATT TAAAGATTTC TTAAATTTAT GTCTAGATAA AAATCCAAAA AAAAGACCAA CTGCAGAATC CTTAATTCAA CATCCTTTTA TTTTTATTCA TAATAAAAAT AGATCATGTA TCAAATCTTA CGCGACACAA CCTAGGGGTA AACAAGGTCC AAAATTGAGC CACAAGGGAT TTAATTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA