SSGCID: Target Page

PlfaA.01374.a

bifunctional farnesyl/geranylgeranyl pyrophosphate synthase, putative (FPPS/GGPPS); apical membrane antigen 1

CENTER ID:	PlfaA.01374.a
ORGANISM:	Plasmodium falciparum 3D7
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlfaA.01374.a.BC1.GE44901	full length		1	376

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlfaA.01374.a.BC1.PW39199	full length		1	376

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PF3D7_1133400
UniProt:	Q8II79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MENEQNNQDS ENGLDYFRSM YDRYRDVFIN HINDYVLEDD IKIIISKYYK LLFDYNCLGG KNNRGILVIL IYEYVKNRDI NCNEWEKVAC IAWCIEILQA SFLVADDIMD KGETRRNKHC WYLLKDVEIK NAVNDVFLLY NAIYKLLDVY LRNDNCYLDL ITSFREATLK TIVGQHLDTN IFSDKYSHID KDIDVNNINI SQENKININM LNFKVYQNII IHKTAYYSFF LPIVCGMQMG GISLDNLLYK KVENIAILMG EYFQVHDDYI DTFGDSKKTG KVGSDIQNNK LTWPLIKAFE LCSQPEKEDI IRNYGKDNVT CIKFINDIYE HYNIRDHYVE YEKKQKMKIL EAINQLHHEG IEYVLKYVMD ILFTGA

NT Sequence

atggagaacg agcagaataa ccaagattca gaaaatggtc tggattactt tagaagtatg tacgatagat acagagatgt gttcataaac catattaatg attacgtatt agaagatgat ataaaaatta taatttcaaa atactataaa ctattatttg attataactg cttagggggt aaaaataata gaggaatttt agttatatta atttatgagt atgtaaagaa tagagatatt aattgtaatg agtgggaaaa agtggcttgt atagcatggt gtatagaaat tttacaagca tcttttttag tagcagatga tattatggat aagggagaaa cacgcagaaa caaacattgt tggtatttgt taaaagacgt tgaaattaag aatgcggtga atgatgtgtt tcttctgtat aacgctatat acaaattact tgatgtatat ttgcgcaatg ataactgtta ccttgattta attacatcct ttagagaagc cactttaaaa actatagtag gacaacattt agatacaaat atattttcag ataaatactc ccatatagac aaagatatag atgttaataa tattaatata tctcaagaga ataaaattaa tataaacatg ttaaatttta aggtttatca aaacattatt attcataaaa ctgcttatta ttcattcttt ttacctattg tttgtggtat gcaaatgggg ggtatatcat tggacaattt attatacaaa aaggtcgaaa atatagcaat tcttatgggg gaatattttc aagtccatga tgattatata gatacctttg gagattctaa aaagacggga aaagttggct cagatattca aaataataaa ttaacgtggc ccttgataaa agcatttgaa ctatgttcac aacctgaaaa agaggacata ataagaaatt atgggaaaga taatgtaaca tgtattaagt ttattaatga tatatatgaa cattataata tcagggatca ttatgtggaa tatgaaaaga agcagaagat gaaaatatta gaagccataa accaattgca tcatgaaggt atagaatatg tcttgaaata cgtaatggac attttgttta caggcgcttg a

Details for PlfaA.01374.a.BC1.GE44901

HARVESTED ON:	11/16/2022
SEQUENCED ON:	5/15/2024
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	Amp
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	Rosetta 2(DE3) Singles(TM) Competent Cells
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHLE VLFQGPMENE QNNQDSENGL DYFRSMYDRY RDVFINHIND YVLEDDIKII ISKYYKLLFD YNCLGGKNNR GILVILIYEY VKNRDINCNE WEKVACIAWC IEILQASFLV ADDIMDKGET RRNKHCWYLL KDVEIKNAVN DVFLLYNAIY KLLDVYLRND NCYLDLITSF REATLKTIVG QHLDTNIFSD KYSHIDKDID VNNINISQEN KININMLNFK VYQNIIIHKT AYYSFFLPIV CGMQMGGISL DNLLYKKVEN IAILMGEYFQ VHDDYIDTFG DSKKTGKVGS DIQNNKLTWP LIKAFELCSQ PEKEDIIRNY GKDNVTCIKF INDIYEHYNI RDHYVEYEKK QKMKILEAIN QLHHEGIEYV LKYVMDILFT GA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggaag tcttgttcca gggacctatg gagaacgagc agaacaatca agacagtgag aatggtctgg attactttcg aagtatgtat gaccgttatc gtgatgtgtt tataaatcac ataaatgatt acgtgctgga agacgacatt aaaataatca tatccaaata ctataagctt ctgtttgact acaactgctt gggcggaaaa aataatcgtg gtatattggt aatcctgatc tacgaatacg tcaaaaaccg tgatattaac tgtaacgaat gggagaaagt agcttgcata gcctggtgta ttgagattct acaagcttcc ttccttgttg ctgatgatat aatggataag ggtgaaacac gacgtaacaa gcactgttgg tacctgctaa aagacgtaga gattaaaaat gctgttaatg atgtgttctt gttatacaat gcaatttaca aactgcttga cgtttattta cgaaacgata actgctacct ggatttgatc acctcattcc gtgaggctac tcttaaaacc attgtaggac agcatttgga caccaacatc ttcagtgaca agtatagtca tatagataag gatatagatg tgaacaacat taatatctca caagaaaaca agattaacat aaacatgctg aattttaagg tgtaccaaaa cataataata cacaagaccg cttactactc attcttccta cccatagtgt gtggtatgca gatgggtgga attagtttgg ataatctttt atataagaaa gtcgagaata tcgcaatatt gatgggcgag tattttcaag ttcacgacga ctacattgac acgttcggag atagtaaaaa gacgggaaaa gttggctcag atatccaaaa caataagtta acatggcctt taataaaggc cttcgagtta tgctctcagc ctgagaaaga agacataata cgtaactatg gcaaagacaa tgtcacctgt atcaaattca taaacgatat ttacgaacac tacaacattc gagatcacta cgttgaatat gaaaaaaagc aaaaaatgaa aatcctggaa gccattaatc aactacacca cgagggtatc gaatatgttc taaaatacgt tatggacata ctttttactg gagctaacca cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for PlfaA.01374.a.BC1.PW39199

PURIFICATION DATe:	3/16/2023
CONCENTRATION:	13.3mg/ml
OBSERVED MW:	46kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	Rosetta 2(DE3) Singles(TM) Competent Cells
VIAL COUNT (approx.):	8
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGAAG TCTTGTTCCA GGGACCTATG GAGAACGAGC AGAACAATCA AGACAGTGAG AATGGTCTGG ATTACTTTCG AAGTATGTAT GACCGTTATC GTGATGTGTT TATAAATCAC ATAAATGATT ACGTGCTGGA AGACGACATT AAAATAATCA TATCCAAATA CTATAAGCTT CTGTTTGACT ACAACTGCTT GGGCGGAAAA AATAATCGTG GTATATTGGT AATCCTGATC TACGAATACG TCAAAAACCG TGATATTAAC TGTAACGAAT GGGAGAAAGT AGCTTGCATA GCCTGGTGTA TTGAGATTCT ACAAGCTTCC TTCCTTGTTG CTGATGATAT AATGGATAAG GGTGAAACAC GACGTAACAA GCACTGTTGG TACCTGCTAA AAGACGTAGA GATTAAAAAT GCTGTTAATG ATGTGTTCTT GTTATACAAT GCAATTTACA AACTGCTTGA CGTTTATTTA CGAAACGATA ACTGCTACCT GGATTTGATC ACCTCATTCC GTGAGGCTAC TCTTAAAACC ATTGTAGGAC AGCATTTGGA CACCAACATC TTCAGTGACA AGTATAGTCA TATAGATAAG GATATAGATG TGAACAACAT TAATATCTCA CAAGAAAACA AGATTAACAT AAACATGCTG AATTTTAAGG TGTACCAAAA CATAATAATA CACAAGACCG CTTACTACTC ATTCTTCCTA CCCATAGTGT GTGGTATGCA GATGGGTGGA ATTAGTTTGG ATAATCTTTT ATATAAGAAA GTCGAGAATA TCGCAATATT GATGGGCGAG TATTTTCAAG TTCACGACGA CTACATTGAC ACGTTCGGAG ATAGTAAAAA GACGGGAAAA GTTGGCTCAG ATATCCAAAA CAATAAGTTA ACATGGCCTT TAATAAAGGC CTTCGAGTTA TGCTCTCAGC CTGAGAAAGA AGACATAATA CGTAACTATG GCAAAGACAA TGTCACCTGT ATCAAATTCA TAAACGATAT TTACGAACAC TACAACATTC GAGATCACTA CGTTGAATAT GAAAAAAAGC AAAAAATGAA AATCCTGGAA GCCATTAATC AACTACACCA CGAGGGTATC GAATATGTTC TAAAATACGT TATGGACATA CTTTTTACTG GAGCTAACCA CGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT