PlfaA.01372.a

Phenylalanine--tRNA ligase, putative, proposed alpha subunit

CENTER ID: PlfaA.01372.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.01372.a.B1.GE39108 full length 1 575
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_0109800
RefSeq: XP_001351028.1
UniProt: Q8I246

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTNNVEEKK NEELYNLLNI LEEEFNLSKI ENKNNNKNDD GDDDDVNKRE DNNNNEYYKK LKDEKGLDVL INEYTTSLKI SKKYNIDHNK VMGMIKKLET LYYIINLIKS FNTYHLSDEG KQYLKEGSPE FIVIKYVIQN NKCTLEDLKK NLGKIGDIGL NTNLKNRTIQ LNKADKCLTC NCNLHDIQDF TQIYLDIINQ YGHDEELLFA HLKNKNHTKN ALNNNEMKNS CKDNNIINNL IQDLKKRKLL EIKKNSYSHV IKTSIFKKDI KKQITDINYL LLKNEEYKKY DIKKYNFFSS GKKINKGNIH LLTKQMRTFK EIFFSLGFEE METHNYVESS FWCFDALYIP QQHPSRDLQD TFFIKEPETC IDKFVDTEYI DNIKRVHTHG DYGSFGWNYK WKLEESKKNV LRTHTTANSC RALFKLAKEY KEAGCIKPKK YFSIDRVFRN ENLDSTHLAE FHQVEGLIID KNIGLSHLIG TLAAFYKHIG IHKLKFKPTF NPYTEPSMEI YGYHEQSKKW LEVGNSGIFR PEMLRSMGFS EEVSVIAWGL SLERPTMIKY NIKNIRDLFG YKSVV
NT Sequence
atgagtacga ataatgtgga agagaaaaaa aatgaggaat tatataattt gctgaacata cttgaagaag aatttaattt gagtaagata gaaaataaaa ataataataa gaatgatgat ggtgatgatg atgatgttaa taaaagagaa gataataata ataatgaata ttacaaaaaa ttaaaagatg agaaaggttt agatgtactt ataaatgaat atacaacatc cttaaagata agtaagaaat ataatattga tcataataaa gtaatgggta tgattaaaaa acttgaaaca ttatattata ttattaatct tattaaatct tttaatacat atcatttgag tgacgaaggt aaacaatatt taaaagaagg ttctcctgaa tttattgtta taaaatatgt aattcaaaat aataaatgta cattagaaga tttaaaaaaa aaccttggaa aaataggaga tataggtcta aatacaaatt taaaaaatcg tacgatacaa ttaaataaag ctgataaatg tttaacatgt aattgtaatt tacatgatat acaagacttt actcaaatat atttggatat aataaatcaa tatggtcatg atgaagaact attatttgcc catttaaaaa ataaaaatca tacgaagaac gcattaaata ataatgaaat gaaaaattct tgtaaagata ataatattat taacaattta atacaagatt taaaaaaaag gaaattatta gaaataaaaa aaaattcata tagtcatgta attaaaacga gcatttttaa aaaagatatt aaaaaacaaa ttacagatat aaattatcta ttattaaaaa atgaagaata taaaaaatat gatataaaaa aatataattt tttttcgagt gggaagaaaa tcaacaaggg aaatattcat cttttaacaa aacaaatgag aacatttaaa gaaatatttt tttctttagg atttgaagaa atggaaactc ataattatgt agaatcatct ttttggtgtt tcgatgcttt atatattcca caacaacatc caagtagaga tttacaagat acctttttta taaaagaacc agaaacttgt atagataaat ttgtagatac agaatatatc gataatatta aaagagtaca tacacatggt gattatggaa gttttggatg gaattataaa tggaaactag aagaaagtaa aaaaaatgtt ttaagaactc atactacagc aaattcttgt agagctttat ttaaactagc caaagaatat aaagaagcag gatgtataaa acccaaaaaa tattttagta tcgatcgtgt ttttagaaat gaaaatttag atagtacaca tttagctgaa tttcatcaag tagaaggact tattatcgat aaaaatattg gactttctca tttaataggt acattagcag cattttataa acacataggt attcacaagc taaaatttaa accgaccttt aatccatata ctgaaccatc catggaaata tatggatatc acgagcagag taaaaagtgg ctagaagttg gaaatagtgg catctttaga ccagaaatgt taagatccat gggattctcc gaagaagtat ccgtaatagc ctggggacta agtctggaga gaccaacaat gattaaatat aacataaaaa atattaggga cctttttgga tacaaaagtg ttgtatga
Details for PlfaA.01372.a.B1.GE39108
HARVESTED ON: 3/31/2015
SEQUENCED ON: 9/30/2016
EXPECTED MW: 69kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM STNNVEEKKN EELYNLLNIL EEEFNLSKIE NKNNNKNDDG DDDDVNKRED NNNNEYYKKL KDEKGLDVLI NEYTTSLKIS KKYNIDHNKV MGMIKKLETL YYIINLIKSF NTYHLSDEGK QYLKEGSPEF IVIKYVIQNN KCTLEDLKKN LGKIGDIGLN TNLKNRTIQL NKADKCLTCN CNLHDIQDFT QIYLDIINQY GHDEELLFAH LKNKNHTKNA LNNNEMKNSC KDNNIINNLI QDLKKRKLLE IKKNSYSHVI KTSIFKKDIK KQITDINYLL LKNEEYKKYD IKKYNFFSSG KKINKGNIHL LTKQMRTFKE IFFSLGFEEM ETHNYVESSF WCFDALYIPQ QHPSRDLQDT FFIKEPETCI DKFVDTEYID NIKRVHTHGD YGSFGWNYKW KLEESKKNVL RTHTTANSCR ALFKLAKEYK EAGCIKPKKY FSIDRVFRNE NLDSTHLAEF HQVEGLIIDK NIGLSHLIGT LAAFYKHIGI HKLKFKPTFN PYTEPSMEIY GYHEQSKKWL EVGNSGIFRP EMLRSMGFSE EVSVIAWGLS LERPTMIKYN IKNIRDLFGY KSVV
Validated NT Sequence
atggctcacc accaccacca ccaccatatg agtacgaata atgtggaaga gaaaaaaaat gaggaattat ataatttgct gaacatactt gaagaagaat ttaatttgag taagatagaa aataaaaata ataataagaa tgatgatggt gatgatgatg atgttaataa aagagaagat aataataata atgaatatta caaaaaatta aaagatgaga aaggtttaga tgtacttata aatgaatata caacatcctt aaagataagt aagaaatata atattgatca taataaagta atgggtatga ttaaaaaact tgaaacatta tattatatta ttaatcttat taaatctttt aatacatatc atttgagtga cgaaggtaaa caatatttaa aagaaggttc tcctgaattt attgttataa aatatgtaat tcaaaataat aaatgtacat tagaagattt aaaaaaaaac cttggaaaaa taggagatat aggtctaaat acaaatttaa aaaatcgtac gatacaatta aataaagctg ataaatgttt aacatgtaat tgtaatttac atgatataca agactttact caaatatatt tggatataat aaatcaatat ggtcatgatg aagaactatt atttgcccat ttaaaaaata aaaatcatac gaagaacgca ttaaataata atgaaatgaa aaattcttgt aaagataata atattatta- acaatttaat acaagattta aaaaaaagga aattattaga aataaaaaaa aattcatata gtcatgtaat taaaacgagc atttttaaaa aagatattaa aaaacaaatt acagatataa attatctatt attaaaaaat gaagaatata aaaaatatga tataaaaaaa tataattttt tttcgagtgg gaagaaaatc aacaagggaa atattcatct tttaacaaaa caaatgagaa catttaaaga aata-ttttt ttctttagga tttgaagaaa tggaaactca taattatgta gaatcatctt tttggtgttt cgatgcttta tatattccac aacaacatcc aagtagagat ttacaagata ccttttttat aaaagaacca gaaacttgta tagataaatt tgtagataca gaatatatcg ataatattaa aagagtacat acacatggtg attatggaag ttttggatgg aattataaat ggaaactaga agaaagtaaa aaaaatgttt taagaactca tactacagca aattcttgta gagctttatt taaactagcc aaagaatata aagaagcagg atgtataaaa cccaaaaaat attttagtat cgatcgtgtt tttagaaatg aaaatttaga tagtacacat ttagctgaat ttcatcaagt agaaggactt attatcgata aaaatattgg actttctcat ttaataggta cattagcagc attttataaa cacataggta ttcacaagct aaaatttaaa ccgaccttta atccatatac tgaaccatcc atggaaatat atggatatca cgagcagagt aaaaagtggc tagaagttgg aaatagtggc atctttagac cagaaatgtt aagatccatg ggattctccg aagaagtatc cgtaatagcc tggggactaa gtctggagag accaacaatg attaaatata acataaaaaa tattagggac ctttttggat acaaaagtgt tgtataa
Expected Protein Sequence
MAHHHHHHMS TNNVEEKKNE ELYNLLNILE EEFNLSKIEN KNNNKNDDGD DDDVNKREDN NNNEYYKKLK DEKGLDVLIN EYTTSLKISK KYNIDHNKVM GMIKKLETLY YIINLIKSFN TYHLSDEGKQ YLKEGSPEFI VIKYVIQNNK CTLEDLKKNL GKIGDIGLNT NLKNRTIQLN KADKCLTCNC NLHDIQDFTQ IYLDIINQYG HDEELLFAHL KNKNHTKNAL NNNEMKNSCK DNNIINNLIQ DLKKRKLLEI KKNSYSHVIK TSIFKKDIKK QITDINYLLL KNEEYKKYDI KKYNFFSSGK KINKGNIHLL TKQMRTFKEI FFSLGFEEME THNYVESSFW CFDALYIPQQ HPSRDLQDTF FIKEPETCID KFVDTEYIDN IKRVHTHGDY GSFGWNYKWK LEESKKNVLR THTTANSCRA LFKLAKEYKE AGCIKPKKYF SIDRVFRNEN LDSTHLAEFH QVEGLIIDKN IGLSHLIGTL AAFYKHIGIH KLKFKPTFNP YTEPSMEIYG YHEQSKKWLE VGNSGIFRPE MLRSMGFSEE VSVIAWGLSL ERPTMIKYNI KNIRDLFGYK SVV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta cgaataatgt ggaagagaaa aaaaatgagg aattatataa tttgctgaac atacttgaag aagaatttaa tttgagtaag atagaaaata aaaataataa taagaatgat gatggtgatg atgatgatgt taataaaaga gaagataata ataataatga atattacaaa aaattaaaag atgagaaagg tttagatgta cttataaatg aatatacaac atccttaaag ataagtaaga aatataatat tgatcataat aaagtaatgg gtatgattaa aaaacttgaa acattatatt atattattaa tcttattaaa tcttttaata catatcattt gagtgacgaa ggtaaacaat atttaaaaga aggttctcct gaatttattg ttataaaata tgtaattcaa aataataaat gtacattaga agatttaaaa aaaaaccttg gaaaaatagg agatataggt ctaaatacaa atttaaaaaa tcgtacgata caattaaata aagctgataa atgtttaaca tgtaattgta atttacatga tatacaagac tttactcaaa tatatttgga tataataaat caatatggtc atgatgaaga actattattt gcccatttaa aaaataaaaa tcatacgaag aacgcattaa ataataatga aatgaaaaat tcttgtaaag ataataatat tattaacaat ttaatacaag atttaaaaaa aaggaaatta ttagaaataa aaaaaaattc atatagtcat gtaattaaaa cgagcatttt taaaaaagat attaaaaaac aaattacaga tataaattat ctattattaa aaaatgaaga atataaaaaa tatgatataa aaaaatataa ttttttttcg agtgggaaga aaatcaacaa gggaaatatt catcttttaa caaaacaaat gagaacattt aaagaaatat ttttttcttt aggatttgaa gaaatggaaa ctcataatta tgtagaatca tctttttggt gtttcgatgc tttatatatt ccacaacaac atccaagtag agatttacaa gatacctttt ttataaaaga accagaaact tgtatagata aatttgtaga tacagaatat atcgataata ttaaaagagt acatacacat ggtgattatg gaagttttgg atggaattat aaatggaaac tagaagaaag taaaaaaaat gttttaagaa ctcatactac agcaaattct tgtagagctt tatttaaact agccaaagaa tataaagaag caggatgtat aaaacccaaa aaatatttta gtatcgatcg tgtttttaga aatgaaaatt tagatagtac acatttagct gaatttcatc aagtagaagg acttattatc gataaaaata ttggactttc tcatttaata ggtacattag cagcatttta taaacacata ggtattcaca agctaaaatt taaaccgacc tttaatccat atactgaacc atccatggaa atatatggat atcacgagca gagtaaaaag tggctagaag ttggaaatag tggcatcttt agaccagaaa tgttaagatc catgggattc tccgaagaag tatccgtaat agcctgggga ctaagtctgg agagaccaac aatgattaaa tataacataa aaaatattag ggaccttttt ggatacaaaa gtgttgtatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat