PlfaA.00856.a

ferrochelatase

CENTER ID:	PlfaA.00856.a
ORGANISM:	Plasmodium falciparum 3D7
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlfaA.00856.a.MB11.GE37868	full length		1	350

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PF3D7_1364900
OrthoMCL:	OG5_127510
RefSeq:	XM_001350324.1
UniProt:	Q8ID58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDVQDFLNCN KLKISKEKIS NLNKSKIGIL ITNLGSPEKL TYWSLYKYLS EFLTDPRVVK LNRFLWLPIL YTFVLPFRSG KVLSKYKSIW IKDGSPLCVN THNQCLALKK ILNEKYNNKV VISYGMRYGE RSIKKGLEYL QKENINKLLV LPLYPQSAEC TVSSTLDCIG KNLKNWSNVP ELRFISGYCL KDIFINTMKE NIENYWELYG KSKKLIISYH SLPIRNVIQG DLYPFFCIES TKKLVKSLNL NKDDYILVFQ SKIKGQQWVK PCIEDTIIRL AKQGYKQIDI VSPSFSSDCL ETLEEIKIHY QQLFRKYSNG NLRYINCLND TTIGIKLIMN LIEQNIIGWV

NT Sequence

atggacgtac aggattttct gaattgtaac aaattaaaaa tatcaaaaga gaaaattagc aaccttaata aaagtaaaat aggaatactg attacaaatt taggaagccc agaaaaacta acctactggt ctttatataa atatttatcg gaatttttga ctgatcctcg agttgtaaaa ttgaacaggt ttttatggct tccaatattg tacacatttg tattaccttt caggagtggc aaagtcttat caaaatataa aagtatatgg ataaaagatg gatcaccatt atgtgttaat actcataatc agtgtttagc tttaaagaag attctaaacg aaaagtacaa taacaaagtt gtcattagtt atggtatgag atatggagaa aggtccataa aaaaaggttt agaatatctc caaaaagaaa acataaataa acttttagtc cttccattat atccacaatc agcagaatgt actgtctctt caactttgga ttgtataggt aaaaatttaa aaaattggag taatgtacct gagctcagat ttatatctgg atattgtttg aaagatattt ttataaatac gatgaaagaa aatattgaaa attattggga actttatggt aaaagtaaaa agttaattat ttcgtatcat agtttaccta tacgaaatgt tattcaagga gatttatatc ctttcttttg tattgaaagt acaaaaaaat tagtgaagtc attaaatctt aataaagatg attatatatt agtctttcaa tcaaaaatta aaggacaaca atgggtaaaa ccatgtattg aagataccat aataaggcta gctaaacaag gatataaaca aatagatatt gttagcccat ctttctcttc agattgttta gaaacattag aagaaataaa aatacattat caacaattat ttcgtaaata ttcaaatgga aatttaagat atataaattg tttgaatgat acaacaattg gtatcaaact aataatgaat cttatagaac aaaatataat aggatgggtg taa

Details for PlfaA.00856.a.MB11.GE37868

HARVESTED ON:	2/25/2014
SEQUENCED ON:	4/16/2014
EXPECTED MW:	86kDa
OBSERVED MW:	86kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	46

Validated AA Sequence

Validated NT Sequence

attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatgaa aatcgaagaa ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg antcttacga ggagagttgg cgaaagatcc acgtattgcn ccactatgga aacgcccaga aagntgaaat catgccgaac atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcan nngccagcgg tcgtcanact gtcgatganc cctnaagacg cncngactan tcganntcga cnnacnncan cacatacaca cctcgggatc gaggnnggat tnnaannncc nggnannnnn ccnnnntnnn nnatgnnnnc ngnnntnnnn acngcaatnn nnncanannt tnnna

Expected Protein Sequence

MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MDVQDFLNCN KLKISKEKIS NLNKSKIGIL ITNLGSPEKL TYWSLYKYLS EFLTDPRVVK LNRFLWLPIL YTFVLPFRSG KVLSKYKSIW IKDGSPLCVN THNQCLALKK ILNEKYNNKV VISYGMRYGE RSIKKGLEYL QKENINKLLV LPLYPQSAEC TVSSTLDCIG KNLKNWSNVP ELRFISGYCL KDIFINTMKE NIENYWELYG KSKKLIISYH SLPIRNVIQG DLYPFFCIES TKKLVKSLNL NKDDYILVFQ SKIKGQQWVK PCIEDTIIRL AKQGYKQIDI VSPSFSSDCL ETLEEIKIHY QQLFRKYSNG NLRYINCLND TTIGIKLIMN LIEQNIIGWV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgga cgtgcaggac ttcctgaact gcaataaact caaaatctct aaagagaaga tttctaacct gaataaatct aaaatcggca tcctgatcac caacctgggc tctccggaaa agctcactta ctggtctctg tacaagtatc tctctgagtt cctgactgac cctcgtgttg taaagctgaa ccgtttcctg tggctcccta tcctctacac ctttgttctg cctttccgtt ctggtaaggt actgtccaag tacaaatcca tctggatcaa agacggttcc ccactctgtg tgaacactca taaccagtgt ctggcactga agaagattct gaacgaaaaa tataacaaca aagttgtgat ctcctacggt atgcgctatg gtgaacgttc catcaaaaag ggcctggaat acctgcagaa ggagaacatt aacaaactgc tcgttctgcc tctgtatccg cagtccgctg aatgcaccgt gtcctctacc ctcgactgta tcggtaagaa cctgaaaaac tggtccaacg tcccggagct gcgctttatc tctggttact gcctcaagga tatcttcatt aacaccatga aggaaaacat cgaaaattat tgggaactgt acggcaaatc caaaaaactg atcatcagct accacagcct gcctatccgt aacgttatcc aaggcgacct gtacccgttc ttctgtatcg aatctaccaa aaaactcgtg aagtctctga acctgaacaa agacgactac atcctggtgt tccaatctaa aatcaaaggt cagcagtggg ttaagccgtg catcgaagac accattatcc gcctggcaaa gcagggctac aaacagatcg atatcgtatc cccgtccttc tcctctgact gcctcgaaac tctcgaagaa atcaaaattc actatcagca gctgttccgc aaatattcta atggcaacct gcgttacatc aactgtctga atgacaccac tatcggcatc aaactgatta tgaacctcat cgagcagaac atcatcggtt gggtttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa