PlfaA.00825.c

Asparagine-tRNA ligase, putative

CENTER ID: PlfaA.00825.c
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PlfaA.00825.c.A2.GE37785 N term domain truncation 92 610
PlfaA.00825.c.B1.GE37770 full length 1 610
PlfaA.00825.c.MB2.GE37876 N term domain truncation 92 610
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PlfaA.00825.c.B1.PW37472 full length 1 610

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_0211800
RefSeq: XP_001349624.1
UniProt: O96198

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCEKDDITVN EEILQKAQEF QVENEKDIKM KKLKPITEGL LKPEVDLLQI SERGSRGRVK ICNVLNVPRS EKEYNNNNSD KVENKYIGKI ITVCGWSKAI RKQGGGRFCF VNLNDGSCHL NLQIVVNQCI ENYEKLLKCG AGCCFRFTGE LIISPVQNDN NKKGLLKENV ELALNNNDIH NFEIYGENLD PQKYPLSKKN HGKEFLREVA HLRPRSYFIS SVIRIRNSLS IATHLFFQSR GFLYIHTPLI TTSDCEGGGE MFTVTTLLNE NGDIRSIPRI NLKNKKKEKR EDILNEKNGK KDHMNDSLNN NTCNNNNNNG NSSSSNIVSS PQYEDNYLID YKKDFFSKQA FLTVSGQLSL ENLCSSMGDV YTFGPTFRAE NSHTSRHLAE FWMIEPEIAF ADLYDNMELA EAYIKYCIDY VLNNNFHDIY YFEENVETNL IKRLKNILNE DFAKITYTNA IEILQNYSDS FEVKVEWGMD LQSEHERFIA EKIFKKPVIV YNYPKDLKAF YMKLNEDNKT VAAMDVLVPK IGEVIGGSQR EDNLERLDKM IKEKKLNIDS YWWYRQLRQY GSHPHAGFGL GFERLIMLVT GVDNIKDTIP FPRYPGHAEF
NT Sequence
atgtgtgaga aagacgatat tactgtgaat gaggaaattt tacaaaaggc acaagagttt caggtggaaa atgagaagga tataaagatg aagaaattaa aaccaataac tgaagggttg ttaaaaccag aggtcgattt attacaaata agtgagagag gtagtcgagg aagagtaaag atatgtaatg tattaaatgt tccaagaagc gaaaaggaat ataataataa taatagtgat aaagtagaaa ataaatatat tggtaaaata attacagtat gtggttggag taaagctata cgtaaacaag gaggtggaag attttgtttt gtgaatttaa atgatggatc atgtcattta aatttacaga ttgttgtaaa tcagtgtata gaaaattatg agaagttatt aaaatgtgga gcaggttgtt gttttcgttt tacaggtgaa ttaattatat cacctgttca gaatgataat aataaaaagg gtttattaaa agaaaatgta gaattagcat taaataataa tgatattcat aattttgaaa tttatggtga aaatttagat ccacagaaat atcctttatc taaaaagaat cacgggaaag aatttctaag agaagtagca catctaagac ctagaagtta ttttattagt tcagtaatta gaattagaaa ctctttatct attgctacgc atttattttt tcaaagtagg ggattcttat atattcatac acctcttata acgacctcag attgtgaagg aggaggagaa atgtttacag tcacgacact tttaaatgaa aatggtgata ttcgtagcat accaaggatt aatttgaaaa acaagaaaaa ggaaaaacgt gaagatattc ttaatgaaaa aaatggaaaa aaggatcata tgaatgatag tttaaataat aatacatgta ataataataa taataatggt aatagtagta gtagtaatat tgtttcatct ccacaatatg aagataatta tcttattgat tataaaaaag atttttttag taaacaagcc tttttaacag taagtggaca attatcatta gaaaatttat gttcatctat gggagatgta tatacattcg gaccaacatt tagagctgaa aattctcata cttctagaca tctagctgaa ttttggatga tagaacccga aatagctttt gcagatttat acgataatat ggagctagcg gaagcctata taaaatattg tatcgactat gttttaaata ataattttca tgatatttat tattttgaag aaaatgttga aacgaattta atcaaaagat taaaaaatat attaaatgaa gattttgcta aaattaccta tactaatgct atagagattt tacaaaatta ttcagattca ttcgaagtga aagttgaatg gggaatggat ttacaatcag aacatgaaag atttatagct gaaaaaatat ttaaaaaacc tgttattgta tataattatc ctaaagactt aaaagccttc tatatgaaat taaatgaaga taataaaaca gttgctgcta tggatgttct cgtacctaaa ataggtgaag tcattggtgg ctcacaaaga gaagataatt tagaacgcct agataaaatg ataaaagaaa aaaaactaaa tatagatagc tattggtggt atcgacaact aagacaatat ggatcacatc cacatgcagg atttggttta ggtttcgaaa gattgattat gttagtaaca ggagttgaca atataaagga taccatacct ttccctagat atcctggtca tgcagaattt taa
Details for PlfaA.00825.c.A2.GE37785
HARVESTED ON: 2/14/2014
SEQUENCED ON: 2/26/2014
EXPECTED MW: 62kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 58
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVCGWSKA IRKQGGGRFC FVNLNDGSCH LNLQIVVNQC IENYEKLLKC GAGCCFRFTG ELIISPVQND NNKKGLLKEN VELALNNNDI HNFEIYGENL DPQKYPLSKK NHGKEFLREV AHLRPRSYFI SSVIRIRNSL SIATHLFFQS RGFLYIHTPL ITTSDCEGGG EMFTVTTLLN ENGDIRSIPR INLKNKKKEK REDILNEKNG KKDHMNDSLN NNTCNNNNNN GNSSSSNIVS SPQYEDNYLI DYKKDFFSKQ AFLTVSGQLS LENLCSSMGD VYTFGXHLEL KISYX
Validated NT Sequence
tcnannatat tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacagt atgtggttgg agtaaagcta tacgtaaaca aggaggtgga agattttgtt ttgtgaattt aaatgatgga tcatgtcatt taaatttaca gattgttgta aatcagtgta tagaaaatta tgagaagtta ttaaaatgtg gagcaggttg ttgttttcgt tttacaggtg aattaattat atcacctgtt cagaatgata ataataaaaa gggtttatta aaagaaaatg tagaattagc attaaataat aatgatattc ataattttga aatttatggt gaaaatttag atccacagaa atatccttta tctaaaaaga atcacgggaa agaatttcta agagaagtag cacatctaag acctagaagt tattttatta gttcagtaat tagaattaga aactctttat ctattgctac gcatttattt tttcaaagta ggggattctt atatattcat acacctctta taacgacctc agattgtgaa ggaggaggag aaatgtttac agtcacgaca cttttaaatg aaaatggtga tattcgtagc ataccaagga ttaatttgaa aaacaagaaa aaggaaaaac gtgaagatat tcttaatgaa aaaaatggaa aaaaggatca tatgaatgat agtttaaata ataatacatg taataataat aataataatg gtaatagtag tagtagtaat attgtttcat ctccacaata tgaagataat tatcttattg attataaaaa agattttttt agtaaacaag cctttttaac agtaagtgga caattatcat tagaaaattt atgttcatct atgggagatg tatatacatt cggacnacat ttagagctga aaatctcata ctnctagaca tctagctgan tttnntgata gaacccgaaa tagctttgca gatttntacg atangnncta gcggnaagcc tatatnaaan nnnntcnann ngnttnnnna annnttcctg gaannnnntt nnnannntnn ncnnnnnnnn naannnnnaa nnntgnnnnt ncnnnncnnc nnnnnnnnnn nnntc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG STVCGWSKAI RKQGGGRFCF VNLNDGSCHL NLQIVVNQCI ENYEKLLKCG AGCCFRFTGE LIISPVQNDN NKKGLLKENV ELALNNNDIH NFEIYGENLD PQKYPLSKKN HGKEFLREVA HLRPRSYFIS SVIRIRNSLS IATHLFFQSR GFLYIHTPLI TTSDCEGGGE MFTVTTLLNE NGDIRSIPRI NLKNKKKEKR EDILNEKNGK KDHMNDSLNN NTCNNNNNNG NSSSSNIVSS PQYEDNYLID YKKDFFSKQA FLTVSGQLSL ENLCSSMGDV YTFGPTFRAE NSHTSRHLAE FWMIEPEIAF ADLYDNMELA EAYIKYCIDY VLNNNFHDIY YFEENVETNL IKRLKNILNE DFAKITYTNA IEILQNYSDS FEVKVEWGMD LQSEHERFIA EKIFKKPVIV YNYPKDLKAF YMKLNEDNKT VAAMDVLVPK IGEVIGGSQR EDNLERLDKM IKEKKLNIDS YWWYRQLRQY GSHPHAGFGL GFERLIMLVT GVDNIKDTIP FPRYPGHAEF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgac agtatgtggt tggagtaaag ctatacgtaa acaaggaggt ggaagatttt gttttgtgaa tttaaatgat ggatcatgtc atttaaattt acagattgtt gtaaatcagt gtatagaaaa ttatgagaag ttattaaaat gtggagcagg ttgttgtttt cgttttacag gtgaattaat tatatcacct gttcagaatg ataataataa aaagggttta ttaaaagaaa atgtagaatt agcattaaat aataatgata ttcataattt tgaaatttat ggtgaaaatt tagatccaca gaaatatcct ttatctaaaa agaatcacgg gaaagaattt ctaagagaag tagcacatct aagacctaga agttatttta ttagttcagt aattagaatt agaaactctt tatctattgc tacgcattta ttttttcaaa gtaggggatt cttatatatt catacacctc ttataacgac ctcagattgt gaaggaggag gagaaatgtt tacagtcacg acacttttaa atgaaaatgg tgatattcgt agcataccaa ggattaattt gaaaaacaag aaaaaggaaa aacgtgaaga tattcttaat gaaaaaaatg gaaaaaagga tcatatgaat gatagtttaa ataataatac atgtaataat aataataata atggtaatag tagtagtagt aatattgttt catctccaca atatgaagat aattatctta ttgattataa aaaagatttt tttagtaaac aagccttttt aacagtaagt ggacaattat cattagaaaa tttatgttca tctatgggag atgtatatac attcggacca acatttagag ctgaaaattc tcatacttct agacatctag ctgaattttg gatgatagaa cccgaaatag cttttgcaga tttatacgat aatatggagc tagcggaagc ctatataaaa tattgtatcg actatgtttt aaataataat tttcatgata tttattattt tgaagaaaat gttgaaacga atttaatcaa aagattaaaa aatatattaa atgaagattt tgctaaaatt acctatacta atgctataga gattttacaa aattattcag attcattcga agtgaaagtt gaatggggaa tggatttaca atcagaacat gaaagattta tagctgaaaa aatatttaaa aaacctgtta ttgtatataa ttatcctaaa gacttaaaag ccttctatat gaaattaaat gaagataata aaacagttgc tgctatggat gttctcgtac ctaaaatagg tgaagtcatt ggtggctcac aaagagaaga taatttagaa cgcctagata aaatgataaa agaaaaaaaa ctaaatatag atagctattg gtggtatcga caactaagac aatatggatc acatccacat gcaggatttg gtttaggttt cgaaagattg attatgttag taacaggagt tgacaatata aaggatacca tacctttccc tagatatcct ggtcatgcag aattttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for PlfaA.00825.c.B1.GE37770
HARVESTED ON: 2/6/2014
SEQUENCED ON: 2/12/2014
EXPECTED MW: 72kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMC EKDDITVNEE ILQKAQEFQV ENEKDIKMKK LKPITEGLLK PEVDLLQISE RGSRGRVKIC NVLNVPRSEK EYNNNNSDKV ENKYIGKIIT VCGWSKAIRK QGGGRFCFVN LNDGSCHLNL QIVVNQCIEN YEKLLKCGAG CCFRFTGELI ISPVQNDNNK KGLLKENVEL ALNNNDIHNF EIYGENLDPQ KYPLSKKNHG KEFLREVAHL RPRSYFISSV IRIRNSLSIA THLFFQSRGF LYIHTPLITT SDCEXGGEMF TVTTLLNENG DIRSIPXINL KNKKRKNVKI FLMKKWKKDH MNDSLNNNTC NNNNNNGNSS SSNIVSSPQY EDNYLIDYKK DFFSKQAFLT VSGQLSLENL CSSMGDVYTF GPTFRAENSH TSRHLAEFWM IEPEIAFADL YDNMELAEAY IKYCIDYVLN NNFHDIYYFE ENVETNLIKR LKNILNEDFA KITYTNAIEI LQNYSDSFEV KVEWGMDLQS EHERFIAEKI FKKPVIVYNY PKDLKAFYMK LNEDNKTVAA MDVLVPKIGE VIGGSQREDN LERLDKMIKE KKLNIDSYWW YRQLRQYGSH PHAGFGLGFE RLIMLVTGVD NIKDTIPFPR YPGHAEF
Validated NT Sequence
atcttactca cttaaaattc tgcatgacca ggatatctag ggaaaggtat ggtatccttt atattgtcaa ctcctgttac taacataatc aatctttcga aacctaaacc aaatcctgca tgtggatgtg atccatattg tcttagttgt cgataccacc aatagctatc tatatttagt tttttttctt ttatcatttt atctaggcgt tctaaattat cttctctttg tgagccacca atgacttcac ctattttagg tacgagaaca tccatagcag caactgtttt attatcttca tttaatttca tatagaaggc ttttaagtct ttaggataat tatatacaat aacaggtttt ttaaatattt tttcagctat aaatctttca tgttctgatt gtaaatccat tccccattca actttcactt cgaatgaatc tgaataattt tgtaaaatct ctatagcatt agtataggta attttagcaa aatcttcatt taatatattt tttaatcttt tgattaaatt cgtttcaaca ttttcttcaa aataataaat atcatgaaaa ttattattta aaacatagtc gatacaatat tttatatagg cttccgctag ctccatatta tcgtataaat ctgcaaaagc tatttcgggt tctatcatcc aaaattcagc tagatgtcta gaagtatgag aattttcagc tctaaatgtt ggtccgaatg tatatacatc tcccatagat gaacataaat tttctaatga taattgtcca cttactgtta aaaaggcttg tttactaaaa aaatcttttt tataatcaat aagataatta tcttcatatt gtggagatga aacaatatta ctactactac tattaccatt attattatta ttattacatg tattattatt taaactatca ttcatatgat cctttttcca ttttttcatt aagaatatct tcacgttttt ccttttcttg tttttcaaat taatccnngg tatgctacga atatcaccat tttcatttaa aagtgtcgtg actgtaaaca tttctcctcc tcnttcacaa tctgaggtcg ttataagagg tgtatgaata tataagaatc ccctactttg aaaaaataaa tgcgtagcaa tagataaaga gtttctaatt ctaattactg aactaataaa ataacttcta ggtcttagat gtgctacttc tcttagaaat tctttcccgt gattcttttt agataaagga tatttctgtg gatctaaatt ttcaccataa atttcaaaat tatgaatatc attattattt aatgctaatt ctacattttc ttttaataaa ccctttttat tattatcatt ctgaacaggt gatataatta attcacctgt aaaacgaaaa caacaacctg ctccacattt taataacttc tcataatttt ctatacactg atttacaaca atctgtaaat ttaaatgaca tgatccatca tttaaattca caaaacaaaa tcttccacct ccttgtttac gtatagcttt actccaacca catactgtaa ttattttacc aatatattta ttttctactt tatcactatt attattatta tattcctttt cgcttcttgg aacatttaat acattacata tctttactct tcctcgacta cctctctcac ttatttgtaa taaatcgacc tctggtttta acaacccttc agttattggt tttaatttct tcatctttat atccttctca ttttccacct gaaactcttg tgccttttgt aaaatttcct cattcacagt aatatcgtct ttctcacaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMC EKDDITVNEE ILQKAQEFQV ENEKDIKMKK LKPITEGLLK PEVDLLQISE RGSRGRVKIC NVLNVPRSEK EYNNNNSDKV ENKYIGKIIT VCGWSKAIRK QGGGRFCFVN LNDGSCHLNL QIVVNQCIEN YEKLLKCGAG CCFRFTGELI ISPVQNDNNK KGLLKENVEL ALNNNDIHNF EIYGENLDPQ KYPLSKKNHG KEFLREVAHL RPRSYFISSV IRIRNSLSIA THLFFQSRGF LYIHTPLITT SDCEGGGEMF TVTTLLNENG DIRSIPRINL KNKKKEKRED ILNEKNGKKD HMNDSLNNNT CNNNNNNGNS SSSNIVSSPQ YEDNYLIDYK KDFFSKQAFL TVSGQLSLEN LCSSMGDVYT FGPTFRAENS HTSRHLAEFW MIEPEIAFAD LYDNMELAEA YIKYCIDYVL NNNFHDIYYF EENVETNLIK RLKNILNEDF AKITYTNAIE ILQNYSDSFE VKVEWGMDLQ SEHERFIAEK IFKKPVIVYN YPKDLKAFYM KLNEDNKTVA AMDVLVPKIG EVIGGSQRED NLERLDKMIK EKKLNIDSYW WYRQLRQYGS HPHAGFGLGF ERLIMLVTGV DNIKDTIPFP RYPGHAEF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgtg agaaagacga tattactgtg aatgaggaaa ttttacaaaa ggcacaagag tttcaggtgg aaaatgagaa ggatataaag atgaagaaat taaaaccaat aactgaaggg ttgttaaaac cagaggtcga tttattacaa ataagtgaga gaggtagtcg aggaagagta aagatatgta atgtattaaa tgttccaaga agcgaaaagg aatataataa taataatagt gataaagtag aaaataaata tattggtaaa ataattacag tatgtggttg gagtaaagct atacgtaaac aaggaggtgg aagattttgt tttgtgaatt taaatgatgg atcatgtcat ttaaatttac agattgttgt aaatcagtgt atagaaaatt atgagaagtt attaaaatgt ggagcaggtt gttgttttcg ttttacaggt gaattaatta tatcacctgt tcagaatgat aataataaaa agggtttatt aaaagaaaat gtagaattag cattaaataa taatgatatt cataattttg aaatttatgg tgaaaattta gatccacaga aatatccttt atctaaaaag aatcacggga aagaatttct aagagaagta gcacatctaa gacctagaag ttattttatt agttcagtaa ttagaattag aaactcttta tctattgcta cgcatttatt ttttcaaagt aggggattct tatatattca tacacctctt ataacgacct cagattgtga aggaggagga gaaatgttta cagtcacgac acttttaaat gaaaatggtg atattcgtag cataccaagg attaatttga aaaacaagaa aaaggaaaaa cgtgaagata ttcttaatga aaaaaatgga aaaaaggatc atatgaatga tagtttaaat aataatacat gtaataataa taataataat ggtaatagta gtagtagtaa tattgtttca tctccacaat atgaagataa ttatcttatt gattataaaa aagatttttt tagtaaacaa gcctttttaa cagtaagtgg acaattatca ttagaaaatt tatgttcatc tatgggagat gtatatacat tcggaccaac atttagagct gaaaattctc atacttctag acatctagct gaattttgga tgatagaacc cgaaatagct tttgcagatt tatacgataa tatggagcta gcggaagcct atataaaata ttgtatcgac tatgttttaa ataataattt tcatgatatt tattattttg aagaaaatgt tgaaacgaat ttaatcaaaa gattaaaaaa tatattaaat gaagattttg ctaaaattac ctatactaat gctatagaga ttttacaaaa ttattcagat tcattcgaag tgaaagttga atggggaatg gatttacaat cagaacatga aagatttata gctgaaaaaa tatttaaaaa acctgttatt gtatataatt atcctaaaga cttaaaagcc ttctatatga aattaaatga agataataaa acagttgctg ctatggatgt tctcgtacct aaaataggtg aagtcattgg tggctcacaa agagaagata atttagaacg cctagataaa atgataaaag aaaaaaaact aaatatagat agctattggt ggtatcgaca actaagacaa tatggatcac atccacatgc aggatttggt ttaggtttcg aaagattgat tatgttagta acaggagttg acaatataaa ggataccata cctttcccta gatatcctgg tcatgcagaa ttttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PlfaA.00825.c.MB2.GE37876
HARVESTED ON: 3/28/2014
SEQUENCED ON: data unavailable
EXPECTED MW: 105kDa
OBSERVED MW: 105kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: failed alignment
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS TVCGWSKAIR KQGGGRFCFV NLNDGSCHLN LQIVVNQCIE NYEKLLKCGA GCCFRFTGEL IISPVQNDNN KKGLLKENVE LALNNNDIHN FEIYGENLDP QKYPLSKKNH GKEFLREVAH LRPRSYFISS VIRIRNSLSI ATHLFFQSRG FLYIHTPLIT TSDCEGGGEM FTVTTLLNEN GDIRSIPRIN LKNKKKEKRE DILNEKNGKK DHMNDSLNNN TCNNNNNNGN SSSSNIVSSP QYEDNYLIDY KKDFFSKQAF LTVSGQLSLE NLCSSMGDVY TFGPTFRAEN SHTSRHLAEF WMIEPEIAFA DLYDNMELAE AYIKYCIDYV LNNNFHDIYY FEENVETNLI KRLKNILNED FAKITYTNAI EILQNYSDSF EVKVEWGMDL QSEHERFIAE KIFKKPVIVY NYPKDLKAFY MKLNEDNKTV AAMDVLVPKI GEVIGGSQRE DNLERLDKMI KEKKLNIDSY WWYRQLRQYG SHPHAGFGLG FERLIMLVTG VDNIKDTIPF PRYPGHAEF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgacagt atgtggttgg agtaaagcta tacgtaaaca aggaggtgga agattttgtt ttgtgaattt aaatgatgga tcatgtcatt taaatttaca gattgttgta aatcagtgta tagaaaatta tgagaagtta ttaaaatgtg gagcaggttg ttgttttcgt tttacaggtg aattaattat atcacctgtt cagaatgata ataataaaaa gggtttatta aaagaaaatg tagaattagc attaaataat aatgatattc ataattttga aatttatggt gaaaatttag atccacagaa atatccttta tctaaaaaga atcacgggaa agaatttcta agagaagtag cacatctaag acctagaagt tattttatta gttcagtaat tagaattaga aactctttat ctattgctac gcatttattt tttcaaagta ggggattctt atatattcat acacctctta taacgacctc agattgtgaa ggaggaggag aaatgtttac agtcacgaca cttttaaatg aaaatggtga tattcgtagc ataccaagga ttaatttgaa aaacaagaaa aaggaaaaac gtgaagatat tcttaatgaa aaaaatggaa aaaaggatca tatgaatgat agtttaaata ataatacatg taataataat aataataatg gtaatagtag tagtagtaat attgtttcat ctccacaata tgaagataat tatcttattg attataaaaa agattttttt agtaaacaag cctttttaac agtaagtgga caattatcat tagaaaattt atgttcatct atgggagatg tatatacatt cggaccaaca tttagagctg aaaattctca tacttctaga catctagctg aattttggat gatagaaccc gaaatagctt ttgcagattt atacgataat atggagctag cggaagccta tataaaatat tgtatcgact atgttttaaa taataatttt catgatattt attattttga agaaaatgtt gaaacgaatt taatcaaaag attaaaaaat atattaaatg aagattttgc taaaattacc tatactaatg ctatagagat tttacaaaat tattcagatt cattcgaagt gaaagttgaa tggggaatgg atttacaatc agaacatgaa agatttatag ctgaaaaaat atttaaaaaa cctgttattg tatataatta tcctaaagac ttaaaagcct tctatatgaa attaaatgaa gataataaaa cagttgctgc tatggatgtt ctcgtaccta aaataggtga agtcattggt ggctcacaaa gagaagataa tttagaacgc ctagataaaa tgataaaaga aaaaaaacta aatatagata gctattggtg gtatcgacaa ctaagacaat atggatcaca tccacatgca ggatttggtt taggtttcga aagattgatt atgttagtaa caggagttga caatataaag gataccatac ctttccctag atatcctggt catgcagaat tttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for PlfaA.00825.c.B1.PW37472
PURIFICATION DATe: 4/3/2014
CONCENTRATION: 13mg/ml
OBSERVED MW: 75kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMC EKDDITVNEE ILQKAQEFQV ENEKDIKMKK LKPITEGLLK PEVDLLQISE RGSRGRVKIC NVLNVPRSEK EYNNNNSDKV ENKYIGKIIT VCGWSKAIRK QGGGRFCFVN LNDGSCHLNL QIVVNQCIEN YEKLLKCGAG CCFRFTGELI ISPVQNDNNK KGLLKENVEL ALNNNDIHNF EIYGENLDPQ KYPLSKKNHG KEFLREVAHL RPRSYFISSV IRIRNSLSIA THLFFQSRGF LYIHTPLITT SDCEXGGEMF TVTTLLNENG DIRSIPXINL KNKKRKNVKI FLMKKWKKDH MNDSLNNNTC NNNNNNGNSS SSNIVSSPQY EDNYLIDYKK DFFSKQAFLT VSGQLSLENL CSSMGDVYTF GPTFRAENSH TSRHLAEFWM IEPEIAFADL YDNMELAEAY IKYCIDYVLN NNFHDIYYFE ENVETNLIKR LKNILNEDFA KITYTNAIEI LQNYSDSFEV KVEWGMDLQS EHERFIAEKI FKKPVIVYNY PKDLKAFYMK LNEDNKTVAA MDVLVPKIGE VIGGSQREDN LERLDKMIKE KKLNIDSYWW YRQLRQYGSH PHAGFGLGFE RLIMLVTGVD NIKDTIPFPR YPGHAEF
Validated NT Sequence
atcttactca cttaaaattc tgcatgacca ggatatctag ggaaaggtat ggtatccttt atattgtcaa ctcctgttac taacataatc aatctttcga aacctaaacc aaatcctgca tgtggatgtg atccatattg tcttagttgt cgataccacc aatagctatc tatatttagt tttttttctt ttatcatttt atctaggcgt tctaaattat cttctctttg tgagccacca atgacttcac ctattttagg tacgagaaca tccatagcag caactgtttt attatcttca tttaatttca tatagaaggc ttttaagtct ttaggataat tatatacaat aacaggtttt ttaaatattt tttcagctat aaatctttca tgttctgatt gtaaatccat tccccattca actttcactt cgaatgaatc tgaataattt tgtaaaatct ctatagcatt agtataggta attttagcaa aatcttcatt taatatattt tttaatcttt tgattaaatt cgtttcaaca ttttcttcaa aataataaat atcatgaaaa ttattattta aaacatagtc gatacaatat tttatatagg cttccgctag ctccatatta tcgtataaat ctgcaaaagc tatttcgggt tctatcatcc aaaattcagc tagatgtcta gaagtatgag aattttcagc tctaaatgtt ggtccgaatg tatatacatc tcccatagat gaacataaat tttctaatga taattgtcca cttactgtta aaaaggcttg tttactaaaa aaatcttttt tataatcaat aagataatta tcttcatatt gtggagatga aacaatatta ctactactac tattaccatt attattatta ttattacatg tattattatt taaactatca ttcatatgat cctttttcca ttttttcatt aagaatatct tcacgttttt ccttttcttg tttttcaaat taatccnngg tatgctacga atatcaccat tttcatttaa aagtgtcgtg actgtaaaca tttctcctcc tcnttcacaa tctgaggtcg ttataagagg tgtatgaata tataagaatc ccctactttg aaaaaataaa tgcgtagcaa tagataaaga gtttctaatt ctaattactg aactaataaa ataacttcta ggtcttagat gtgctacttc tcttagaaat tctttcccgt gattcttttt agataaagga tatttctgtg gatctaaatt ttcaccataa atttcaaaat tatgaatatc attattattt aatgctaatt ctacattttc ttttaataaa ccctttttat tattatcatt ctgaacaggt gatataatta attcacctgt aaaacgaaaa caacaacctg ctccacattt taataacttc tcataatttt ctatacactg atttacaaca atctgtaaat ttaaatgaca tgatccatca tttaaattca caaaacaaaa tcttccacct ccttgtttac gtatagcttt actccaacca catactgtaa ttattttacc aatatattta ttttctactt tatcactatt attattatta tattcctttt cgcttcttgg aacatttaat acattacata tctttactct tcctcgacta cctctctcac ttatttgtaa taaatcgacc tctggtttta acaacccttc agttattggt tttaatttct tcatctttat atccttctca ttttccacct gaaactcttg tgccttttgt aaaatttcct cattcacagt aatatcgtct ttctcacaca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMC EKDDITVNEE ILQKAQEFQV ENEKDIKMKK LKPITEGLLK PEVDLLQISE RGSRGRVKIC NVLNVPRSEK EYNNNNSDKV ENKYIGKIIT VCGWSKAIRK QGGGRFCFVN LNDGSCHLNL QIVVNQCIEN YEKLLKCGAG CCFRFTGELI ISPVQNDNNK KGLLKENVEL ALNNNDIHNF EIYGENLDPQ KYPLSKKNHG KEFLREVAHL RPRSYFISSV IRIRNSLSIA THLFFQSRGF LYIHTPLITT SDCEGGGEMF TVTTLLNENG DIRSIPRINL KNKKKEKRED ILNEKNGKKD HMNDSLNNNT CNNNNNNGNS SSSNIVSSPQ YEDNYLIDYK KDFFSKQAFL TVSGQLSLEN LCSSMGDVYT FGPTFRAENS HTSRHLAEFW MIEPEIAFAD LYDNMELAEA YIKYCIDYVL NNNFHDIYYF EENVETNLIK RLKNILNEDF AKITYTNAIE ILQNYSDSFE VKVEWGMDLQ SEHERFIAEK IFKKPVIVYN YPKDLKAFYM KLNEDNKTVA AMDVLVPKIG EVIGGSQRED NLERLDKMIK EKKLNIDSYW WYRQLRQYGS HPHAGFGLGF ERLIMLVTGV DNIKDTIPFP RYPGHAEF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTGTG AGAAAGACGA TATTACTGTG AATGAGGAAA TTTTACAAAA GGCACAAGAG TTTCAGGTGG AAAATGAGAA GGATATAAAG ATGAAGAAAT TAAAACCAAT AACTGAAGGG TTGTTAAAAC CAGAGGTCGA TTTATTACAA ATAAGTGAGA GAGGTAGTCG AGGAAGAGTA AAGATATGTA ATGTATTAAA TGTTCCAAGA AGCGAAAAGG AATATAATAA TAATAATAGT GATAAAGTAG AAAATAAATA TATTGGTAAA ATAATTACAG TATGTGGTTG GAGTAAAGCT ATACGTAAAC AAGGAGGTGG AAGATTTTGT TTTGTGAATT TAAATGATGG ATCATGTCAT TTAAATTTAC AGATTGTTGT AAATCAGTGT ATAGAAAATT ATGAGAAGTT ATTAAAATGT GGAGCAGGTT GTTGTTTTCG TTTTACAGGT GAATTAATTA TATCACCTGT TCAGAATGAT AATAATAAAA AGGGTTTATT AAAAGAAAAT GTAGAATTAG CATTAAATAA TAATGATATT CATAATTTTG AAATTTATGG TGAAAATTTA GATCCACAGA AATATCCTTT ATCTAAAAAG AATCACGGGA AAGAATTTCT AAGAGAAGTA GCACATCTAA GACCTAGAAG TTATTTTATT AGTTCAGTAA TTAGAATTAG AAACTCTTTA TCTATTGCTA CGCATTTATT TTTTCAAAGT AGGGGATTCT TATATATTCA TACACCTCTT ATAACGACCT CAGATTGTGA AGGAGGAGGA GAAATGTTTA CAGTCACGAC ACTTTTAAAT GAAAATGGTG ATATTCGTAG CATACCAAGG ATTAATTTGA AAAACAAGAA AAAGGAAAAA CGTGAAGATA TTCTTAATGA AAAAAATGGA AAAAAGGATC ATATGAATGA TAGTTTAAAT AATAATACAT GTAATAATAA TAATAATAAT GGTAATAGTA GTAGTAGTAA TATTGTTTCA TCTCCACAAT ATGAAGATAA TTATCTTATT GATTATAAAA AAGATTTTTT TAGTAAACAA GCCTTTTTAA CAGTAAGTGG ACAATTATCA TTAGAAAATT TATGTTCATC TATGGGAGAT GTATATACAT TCGGACCAAC ATTTAGAGCT GAAAATTCTC ATACTTCTAG ACATCTAGCT GAATTTTGGA TGATAGAACC CGAAATAGCT TTTGCAGATT TATACGATAA TATGGAGCTA GCGGAAGCCT ATATAAAATA TTGTATCGAC TATGTTTTAA ATAATAATTT TCATGATATT TATTATTTTG AAGAAAATGT TGAAACGAAT TTAATCAAAA GATTAAAAAA TATATTAAAT GAAGATTTTG CTAAAATTAC CTATACTAAT GCTATAGAGA TTTTACAAAA TTATTCAGAT TCATTCGAAG TGAAAGTTGA ATGGGGAATG GATTTACAAT CAGAACATGA AAGATTTATA GCTGAAAAAA TATTTAAAAA ACCTGTTATT GTATATAATT ATCCTAAAGA CTTAAAAGCC TTCTATATGA AATTAAATGA AGATAATAAA ACAGTTGCTG CTATGGATGT TCTCGTACCT AAAATAGGTG AAGTCATTGG TGGCTCACAA AGAGAAGATA ATTTAGAACG CCTAGATAAA ATGATAAAAG AAAAAAAACT AAATATAGAT AGCTATTGGT GGTATCGACA ACTAAGACAA TATGGATCAC ATCCACATGC AGGATTTGGT TTAGGTTTCG AAAGATTGAT TATGTTAGTA ACAGGAGTTG ACAATATAAA GGATACCATA CCTTTCCCTA GATATCCTGG TCATGCAGAA TTTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT