PlfaA.00641.a

Casein kinase 1, PfCK1

CENTER ID: PlfaA.00641.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.00641.a.B1.GE37851 full length 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1136500.2
RefSeq: XP_002585436.1
UniProt: C6S3F7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEIRVANKYA LGKKLGSGSF GDIYVAKDIV TMEEFAVKLE STRSKHPQLL YESKLYKILG GGIGVPKVYW YGIEGDFTIM VLDLLGPSLE DLFTLCNRKF SLKTVLMTAD QMLNRIEYVH SKNFIHRDIK PDNFLIGRGK KVTLIHIIDF GLAKKYRDSR SHTHIPYKEG KNLTGTARYA SINTHLGIEQ SRRDDIEALG YVLMYFLRGS LPWQGLKAIS KKDKYDKIME KKISTSVEVL CRNASFEFVT YLNYCRSLRF EDRPDYTYLR RLLKDLFIRE GFTYDFLFDW TCVYASEKDK KKMLENKNRF DQTADQEGRD QRNN
NT Sequence
atggaaatta gagtggcaaa taaatatgct ttaggaaaaa aattagggag tggttccttt ggtgatatat atgttgccaa ggatattgta acgatggaag aatttgctgt aaaattagaa tcaacacgat caaaacatcc ccaactattg tatgagtcaa aattatataa aatattagga ggaggaattg gtgtacctaa agtatattgg tacggtatag aaggggattt tactatcatg gttcttgatt tattaggccc atcccttgaa gatttattta ccttatgtaa tagaaaattt tctttaaaga ctgtccttat gacagcagat caaatgttaa atagaattga atatgtgcat tcaaaaaatt ttatacatag agatattaaa ccagataact ttttaatagg acgagggaaa aaagttacct taatacatat tattgatttt ggtttagcaa aaaaatatag agattcaaga tcacatactc atattccata taaagaaggg aaaaatttaa caggaacagc aagatatgca agtataaata cacacttagg aattgaacaa tctcgtaggg atgatattga agcccttgga tatgttctca tgtattttct aagaggaagt ttaccatggc aaggtctaaa ggctatatct aagaaggata aatatgataa aattatggaa aaaaaaatat caacatcagt ggaagttttg tgtagaaatg caagcttcga attcgtaaca tatttaaatt attgccgatc attaagattt gaagatagac ccgattatac atatttaaga agacttttaa aggacttgtt tataagagag ggatttactt atgacttttt atttgactgg acatgtgtat atgcctccga aaaggataag aagaagatgt tagaaaataa aaaccgtttt gatcaaacag cagatcaaga aggaagagat caacgaaata attga
Details for PlfaA.00641.a.B1.GE37851
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME IRVANKYALG KKLGSGSFGD IYVAKDIVTM EEFAVKLEST RSKHPQLLYE SKLYKILGGG IGVPKVYWYG IEGDFTIMVL DLLGPSLEDL FTLCNRKFSL KTVLMTADQM LNRIEYVHSK NFIHRDIKPD NFLIGRGKKV TLIHIIDFGL AKKYRDSRSH THIPYKEGKN LTGTARYASI NTHLGIEQSR RDDIEALGYV LMYFLRGSLP WQGLKAISKK DKYDKIMEKK ISTSVEVLCR NASFEFVTYL NYCRSLRFED RPDYTYLRRL LKDLFIREGF TYDFLFDWTC VYASEKDKKK MLENKNRFDQ TADQEGEITK YXE
Validated NT Sequence
annncnctag atnttttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg gaaattagag tggcaaataa atatgcttta ggaaaaaaat tagggagtgg ttcctttggt gatatatatg ttgccaagga tattgtaacg atggaagaat ttgctgtaaa attagaatca acacgatcaa aacatcccca actattgtat gagtcaaaat tatataaaat attaggagga ggaattggtg tacctaaagt atattggtac ggtatagaag gggattttac tatcatggtt cttgatttat taggcccatc ccttgaagat ttatttacct tatgtaatag aaaattttct ttaaagactg tccttatgac agcagatcaa atgttaaata gaattgaata tgtgcattca aaaaatttta tacatagaga tattaaacca gataactttt taataggacg agggaaaaaa gttaccttaa tacatattat tgattttggt ttagcaaaaa aatatagaga ttcaagatca catactcata ttccatataa agaagggaaa aatttaacag gaacagcaag atatgcaagt ataaatacac acttaggaat tgaacaatct cgtagggatg atattgaagc cctaggatat gttctcatgt attttctaag aggaagttta ccatggcaag gtctaaaggc tatatctaag aaggataaat atgataaaat tatggaaaaa aaaatatcaa catcagtgga agttttgtgt agaaatgcaa gcttcgaatt cgtaacatat ttaaattatt gccgatcatt aagatttgaa gatagacccg attatacata tttaagaaga cttttaaagg acttgtttat aagagaggga tttacttatg actttttatt tgactggaca tgtgtatatg cctccgaaaa ggataagaag aagatgttag aaaataaaaa ccgttttgat caaacagcag atcaagaagg agagatcacg aaatatnntg agtaatnnnn cnnnnnnntg nnngnnnnnn nnnnnannnn tancnaannn nnnnnnnnnn nnnnnnntac nngnnnnnnn nt
Expected Protein Sequence
MAHHHHHHME IRVANKYALG KKLGSGSFGD IYVAKDIVTM EEFAVKLEST RSKHPQLLYE SKLYKILGGG IGVPKVYWYG IEGDFTIMVL DLLGPSLEDL FTLCNRKFSL KTVLMTADQM LNRIEYVHSK NFIHRDIKPD NFLIGRGKKV TLIHIIDFGL AKKYRDSRSH THIPYKEGKN LTGTARYASI NTHLGIEQSR RDDIEALGYV LMYFLRGSLP WQGLKAISKK DKYDKIMEKK ISTSVEVLCR NASFEFVTYL NYCRSLRFED RPDYTYLRRL LKDLFIREGF TYDFLFDWTC VYASEKDKKK MLENKNRFDQ TADQEGRDQR NN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa ttagagtggc aaataaatat gctttaggaa aaaaattagg gagtggttcc tttggtgata tatatgttgc caaggatatt gtaacgatgg aagaatttgc tgtaaaatta gaatcaacac gatcaaaaca tccccaacta ttgtatgagt caaaattata taaaatatta ggaggaggaa ttggtgtacc taaagtatat tggtacggta tagaagggga ttttactatc atggttcttg atttattagg cccatccctt gaagatttat ttaccttatg taatagaaaa ttttctttaa agactgtcct tatgacagca gatcaaatgt taaatagaat tgaatatgtg cattcaaaaa attttataca tagagatatt aaaccagata actttttaat aggacgaggg aaaaaagtta ccttaataca tattattgat tttggtttag caaaaaaata tagagattca agatcacata ctcatattcc atataaagaa gggaaaaatt taacaggaac agcaagatat gcaagtataa atacacactt aggaattgaa caatctcgta gggatgatat tgaagccctt ggatatgttc tcatgtattt tctaagagga agtttaccat ggcaaggtct aaaggctata tctaagaagg ataaatatga taaaattatg gaaaaaaaaa tatcaacatc agtggaagtt ttgtgtagaa atgcaagctt cgaattcgta acatatttaa attattgccg atcattaaga tttgaagata gacccgatta tacatattta agaagacttt taaaggactt gtttataaga gagggattta cttatgactt tttatttgac tggacatgtg tatatgcctc cgaaaaggat aagaagaaga tgttagaaaa taaaaaccgt tttgatcaaa cagcagatca agaaggaaga gatcaacgaa ataattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat