PlfaA.00433.b

Serine/threonine protein kinase (EC 2.7.11.1)

CENTER ID: PlfaA.00433.b
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.00433.b.B1.GE43351 full length 1 367
PlfaA.00433.b.B4.GE43354 truncation 3 71 367
PlfaA.00433.b.B5.GE43355 truncation 4 92 367
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1315100
UniProt: Q8IEG4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYLGSIKNFE DDFLCKGEYK QVYLSLEQNE NNEAIEIDIC CDNNIYDILQ FCREKYGIYG EYIIDSCGNV IHSIKDIIDG DTLYLKEKKE DMNFFSKIKN NFIVNDYIVE KRIGSGGFGI VFQGVHIQTK QKVALKFIPK SNFLDVTDVH RVFIEIQTLR GLIHNNIIKM YDVNHFQNYV CLIMEYAING DLKNYIKNKF NGFLSEKEAH DLFLQIVKGV YYCHSKHIVH RDLKLENILL DEKMTCKIAD FGLSDFVNVD QNIKTEAGTK AYIAPEIIFN QTINYSVFKL DIWSLGILLF IMTQGFAPFK YMEKELKNFE SNTLNYANDI SDDLKDLISL MLNVDPNKRP IIVEILNHRW FENYKES
NT Sequence
atgtatttgg gaagtataaa aaatttcgaa gacgactttt tatgtaaagg tgaatataaa caggtctatt tatccctaga acaaaatgag aacaacgagg ctatagaaat agatatatgc tgtgataata atatttatga tatacttcaa ttttgtagag aaaagtatgg tatatatggt gaatatatta ttgatagttg tggtaatgta atacattcga taaaagatat aatagatggt gataccttat atttgaaaga aaagaaagaa gatatgaatt tcttttcaaa aataaaaaat aattttattg taaacgatta tatagtagaa aagagaatag gttctggtgg atttggtatt gtatttcaag gtgtacatat acaaacaaaa caaaaggtag cattaaaatt tattcctaag agtaattttt tagatgtaac agatgtacat agagtattta ttgaaattca aacattgaga ggattaatac ataataatat tattaaaatg tatgatgtaa atcattttca gaattatgta tgcttaataa tggaatatgc aataaatgga gatttaaaaa attatattaa aaataagttt aatggatttt tatctgaaaa agaagctcat gatttatttc ttcaaattgt taaaggggta tattattgtc attctaaaca tatagtacat cgagatttaa aattagaaaa tatattatta gatgaaaaaa tgacatgtaa aatagctgat tttggattat cagattttgt taatgtagat caaaatatta aaacagaagc aggaacaaaa gcatatatag caccagaaat catttttaat caaacaatta attattcagt ttttaaatta gatatatgga gtttaggaat tttattattt attatgacac aaggatttgc accttttaag tatatggaaa aagaactcaa aaattttgaa agtaatacac taaattatgc aaacgatata tcagatgatt tgaaagattt aatttcatta atgttgaacg ttgatcctaa taaaagacca atcatagtag agatactaaa tcataggtgg tttgaaaatt ataaggagtc ataa
Details for PlfaA.00433.b.B1.GE43351
HARVESTED ON: 7/31/2019
SEQUENCED ON: 8/7/2019
EXPECTED MW: 44kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 50
Validated AA Sequence
MAHHHHHHMY LGSIKNFEDD FLCKGEYKQV YLSLEQNENN EAIEIDICCD NNIYDILQFC REKYGIYGEY IIDSCGNVIH SIKDIIDGDT LYLKEKKEDM NFFSKIKNNF IVNDYIVEKR IGSGGFGIVF QGVHIQTKQK VALKFIPKSN FLDVTDVHRV FIEIQTLRGL IHNNIIXMYD VNHFQIMYA
Validated NT Sequence
gnnnnnnnnn nnnnnntnnn ncnactcnct atagggagac cncaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tgtatttggg aagtataaaa aatttcgaag acgacttttt atgtaaaggt gaatataaac aggtctattt atccctagaa caaaatgaga acaacgaggc tatagaaata gatatatgct gtgataataa tatttatgat atacttcaat tttgtagaga aaagtatggt atatatggtg aatatattat tgatagttgt ggtaatgtaa tacattcgat aaaagatata atagatggtg ataccttata tttgaaagaa aagaaagaag atatgaattt cttttcaaaa ataaaaaata attttattgt aaacgattat atagtagaaa agagaatagg ttctggtgga tttggtattg tatttcaagg tgtacatata caaacaaaac aaaaggtagc attaaaattt attcctaaga gtaatttttt agatgtaaca gatgtacata gagtatttat tgaaattcaa acattgagag gattaataca taataatatt attanaatgt atgatgtaaa tcattttcaa attatgtatg cttaaaaatg gaatatgcaa taaatggaga tttaaaaaat tatattnaaa ataagtttnn tggantttta tctgaaaaan aanctcatga tttatttctt cnaattgnta aaggggtata ttattgncat tctaaacata tagngacatc nagacttaaa attannaaaa tatattatta natganaaaa atgacatgnn naatagctga ttttgnanta tcnnatttng nnanngtaat caaaatatta aaacagnagn nggaacnaaa ncnatatanc nccnnancnn ntttaatcaa ananntaatn tanttcagtt tttcaantaa aanatnggga gtttaggnan ntnnnntnnt ntaatnanga cncnnngnat ntngncnncn tttnngnnnn tggnnnnnca ancnncnnaa tnnnnnaang naanctcnta acnntctnnn ncaccnnann nnnncnnang nnnnnnnnnn ntnanttnnn nnnnngnnng cagnnnnnan cccnnannaa nnnnccnnnn cnnna
Expected Protein Sequence
MAHHHHHHMY LGSIKNFEDD FLCKGEYKQV YLSLEQNENN EAIEIDICCD NNIYDILQFC REKYGIYGEY IIDSCGNVIH SIKDIIDGDT LYLKEKKEDM NFFSKIKNNF IVNDYIVEKR IGSGGFGIVF QGVHIQTKQK VALKFIPKSN FLDVTDVHRV FIEIQTLRGL IHNNIIKMYD VNHFQNYVCL IMEYAINGDL KNYIKNKFNG FLSEKEAHDL FLQIVKGVYY CHSKHIVHRD LKLENILLDE KMTCKIADFG LSDFVNVDQN IKTEAGTKAY IAPEIIFNQT INYSVFKLDI WSLGILLFIM TQGFAPFKYM EKELKNFESN TLNYANDISD DLKDLISLML NVDPNKRPII VEILNHRWFE NYKES
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtatt tgggaagtat aaaaaatttc gaagacgact ttttatgtaa aggtgaatat aaacaggtct atttatccct agaacaaaat gagaacaacg aggctataga aatagatata tgctgtgata ataatattta tgatatactt caattttgta gagaaaagta tggtatatat ggtgaatata ttattgatag ttgtggtaat gtaatacatt cgataaaaga tataatagat ggtgatacct tatatttgaa agaaaagaaa gaagatatga atttcttttc aaaaataaaa aataatttta ttgtaaacga ttatatagta gaaaagagaa taggttctgg tggatttggt attgtatttc aaggtgtaca tatacaaaca aaacaaaagg tagcattaaa atttattcct aagagtaatt ttttagatgt aacagatgta catagagtat ttattgaaat tcaaacattg agaggattaa tacataataa tattattaaa atgtatgatg taaatcattt tcagaattat gtatgcttaa taatggaata tgcaataaat ggagatttaa aaaattatat taaaaataag tttaatggat ttttatctga aaaagaagct catgatttat ttcttcaaat tgttaaaggg gtatattatt gtcattctaa acatatagta catcgagatt taaaattaga aaatatatta ttagatgaaa aaatgacatg taaaatagct gattttggat tatcagattt tgttaatgta gatcaaaata ttaaaacaga agcaggaaca aaagcatata tagcaccaga aatcattttt aatcaaacaa ttaattattc agtttttaaa ttagatatat ggagtttagg aattttatta tttattatga cacaaggatt tgcacctttt aagtatatgg aaaaagaact caaaaatttt gaaagtaata cactaaatta tgcaaacgat atatcagatg atttgaaaga tttaatttca ttaatgttga acgttgatcc taataaaaga ccaatcatag tagagatact aaatcatagg tggtttgaaa attataagga gtcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for PlfaA.00433.b.B4.GE43354
HARVESTED ON: 7/31/2019
SEQUENCED ON: 8/7/2019
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM IHSIKDIIDG DTLYLKEKKE DMNFFSKIKN NFIVNDYIVE KRIGSGGFGI VFQGVHIQTK QKVALKFIPK SNFLDVTDVH RVFIEIQTLR GLIHNNIIKM YDVNHFQNYV CLIMEYAING DLKNYIKNKF NGFLSEKEAH DLFLQIVKGV YYCHSKHIVH RDLKLENILL DEKMTCKIAD FGLSDFVNVD QNIKTEAGTK AYIAPEIIFN QTINYSVFKL DIWSLGILLF IMTQGFAPFK YMEKELKNFE SNTLNYANDI SDDLKDLISL MLNVDPNKRP IIVEILNHKW FENYKES
Validated NT Sequence
tactcnctta tgactcctta taattttcaa accatttatg atttagtatc tctactatga ttggtctttt attaggatca acgttcaaca ttaatgaaat taaatctttc aaatcatctg atatatcgtt tgcataattt agtgtattac tttcaaaatt tttgagttct ttttccatat acttaaaagg tgcaaatcct tgtgtcataa taaataataa aattcctaaa ctccatatat ctaatttaaa aactgaataa ttaattgttt gattaaaaat gatttctggt gctatatatg cttttgttcc tgcttctgtt ttaatatttt gatctacatt aacaaaatct gataatccaa aatcagctat tttacatgtc attttttcat ctaataatat attttctaat tttaaatctc gatgtactat atgtttagaa tgacaataat ataccccttt aacaatttga agaaataaat catgagcttc tttttcagat aaaaatccat taaacttatt tttaatataa ttttttaaat ctccatttat tgcatattcc attattaagc atacataatt ctgaaaatga tttacatcat acattttaat aatattatta tgtattaatc ctctcaatgt ttgaatttca ataaatactc tatgtacatc tgttacatct aaaaaattac tcttaggaat aaattttaat gctacctttt gttttgtttg tatatgtaca ccttgaaata caataccaaa tccaccagaa cctattctct tttctactat ataatcgttt acaataaaat tattttttat ttttgaaaag aaattcatat cttctttctt ttctttcaaa tataaggtat caccatctat tatatctttt atcgaatgta tcatatggtg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHIH SIKDIIDGDT LYLKEKKEDM NFFSKIKNNF IVNDYIVEKR IGSGGFGIVF QGVHIQTKQK VALKFIPKSN FLDVTDVHRV FIEIQTLRGL IHNNIIKMYD VNHFQNYVCL IMEYAINGDL KNYIKNKFNG FLSEKEAHDL FLQIVKGVYY CHSKHIVHRD LKLENILLDE KMTCKIADFG LSDFVNVDQN IKTEAGTKAY IAPEIIFNQT INYSVFKLDI WSLGILLFIM TQGFAPFKYM EKELKNFESN TLNYANDISD DLKDLISLML NVDPNKRPII VEILNHRWFE NYKES
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatacatt cgataaaaga tataatagat ggtgatacct tatatttgaa agaaaagaaa gaagatatga atttcttttc aaaaataaaa aataatttta ttgtaaacga ttatatagta gaaaagagaa taggttctgg tggatttggt attgtatttc aaggtgtaca tatacaaaca aaacaaaagg tagcattaaa atttattcct aagagtaatt ttttagatgt aacagatgta catagagtat ttattgaaat tcaaacattg agaggattaa tacataataa tattattaaa atgtatgatg taaatcattt tcagaattat gtatgcttaa taatggaata tgcaataaat ggagatttaa aaaattatat taaaaataag tttaatggat ttttatctga aaaagaagct catgatttat ttcttcaaat tgttaaaggg gtatattatt gtcattctaa acatatagta catcgagatt taaaattaga aaatatatta ttagatgaaa aaatgacatg taaaatagct gattttggat tatcagattt tgttaatgta gatcaaaata ttaaaacaga agcaggaaca aaagcatata tagcaccaga aatcattttt aatcaaacaa ttaattattc agtttttaaa ttagatatat ggagtttagg aattttatta tttattatga cacaaggatt tgcacctttt aagtatatgg aaaaagaact caaaaatttt gaaagtaata cactaaatta tgcaaacgat atatcagatg atttgaaaga tttaatttca ttaatgttga acgttgatcc taataaaaga ccaatcatag tagagatact aaatcatagg tggtttgaaa attataagga gtcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for PlfaA.00433.b.B5.GE43355
HARVESTED ON: 7/31/2019
SEQUENCED ON: 8/7/2019
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN FFSKIKNNFI VNDYIVEKRI GSGGFGIVFQ GVHIQTKQKV ALKFIPKSNF LDVTDVHRVF IEIQTLRGLI HNNIIKMYDV NHFQNYVCLI MEYAINGDLK NYIKNKFNGF LSEKEAHDLF LQIVKGVYYC HSKHIVHRDL KLENILLDEK MTCKIADFGL SDFVNVDQNI KTEAGTKAYI APEIIFNQTI NYSVFKLDIW SLGILLFIMT QGFAPFKYME KELKNFESNT LNYANDISDD LKDLISLMLN VDPNKRPIIV EILNHRWFEN YKES
Validated NT Sequence
atcttactca cttatgactc cttataattt tcaaaccacc tatgatttag tatctctact atgattggtc ttttattagg atcaacgttc aacattaatg aaattaaatc tttcaaatca tctgatatat cgtttgcata atttagtgta ttactttcaa aatttttgag ttctttttcc atatacttaa aaggtgcaaa tccttgtgtc ataataaata ataaaattcc taaactccat atatctaatt taaaaactga ataattaatt gtttgattaa aaatgatttc tggtgctata tatgcttttg ttcctgcttc tgttttaata ttttgatcta cattaacaaa atctgataat ccaaaatcag ctattttaca tgtcattttt tcatctaata atatattttc taattttaaa tctcgatgta ctatatgttt agaatgacaa taatataccc ctttaacaat ttgaagaaat aaatcatgag cttctttttc agataaaaat ccattaaact tatttttaat ataatttttt aaatctccat ttattgcata ttccattatt aagcatacat aattctgaaa atgatttaca tcatacattt taataatatt attatgtatt aatcctctca atgtttgaat ttcaataaat actctatgta catctgttac atctaaaaaa ttactcttag gaataaattt taatgctacc ttttgttttg tttgtatatg tacaccttga aatacaatac caaatccacc agaacctatt ctcttttcta ctatataatc gtttacaata aaattatttt ttatttttga aaagaaattc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMN FFSKIKNNFI VNDYIVEKRI GSGGFGIVFQ GVHIQTKQKV ALKFIPKSNF LDVTDVHRVF IEIQTLRGLI HNNIIKMYDV NHFQNYVCLI MEYAINGDLK NYIKNKFNGF LSEKEAHDLF LQIVKGVYYC HSKHIVHRDL KLENILLDEK MTCKIADFGL SDFVNVDQNI KTEAGTKAYI APEIIFNQTI NYSVFKLDIW SLGILLFIMT QGFAPFKYME KELKNFESNT LNYANDISDD LKDLISLMLN VDPNKRPIIV EILNHRWFEN YKES
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt tcttttcaaa aataaaaaat aattttattg taaacgatta tatagtagaa aagagaatag gttctggtgg atttggtatt gtatttcaag gtgtacatat acaaacaaaa caaaaggtag cattaaaatt tattcctaag agtaattttt tagatgtaac agatgtacat agagtattta ttgaaattca aacattgaga ggattaatac ataataatat tattaaaatg tatgatgtaa atcattttca gaattatgta tgcttaataa tggaatatgc aataaatgga gatttaaaaa attatattaa aaataagttt aatggatttt tatctgaaaa agaagctcat gatttatttc ttcaaattgt taaaggggta tattattgtc attctaaaca tatagtacat cgagatttaa aattagaaaa tatattatta gatgaaaaaa tgacatgtaa aatagctgat tttggattat cagattttgt taatgtagat caaaatatta aaacagaagc aggaacaaaa gcatatatag caccagaaat catttttaat caaacaatta attattcagt ttttaaatta gatatatgga gtttaggaat tttattattt attatgacac aaggatttgc accttttaag tatatggaaa aagaactcaa aaattttgaa agtaatacac taaattatgc aaacgatata tcagatgatt tgaaagattt aatttcatta atgttgaacg ttgatcctaa taaaagacca atcatagtag agatactaaa tcataggtgg tttgaaaatt ataaggagtc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat