SSGCID: Target Page

PlfaA.00425.a

Elongation factor 1-alpha

CENTER ID:	PlfaA.00425.a
ORGANISM:	Plasmodium falciparum 3D7
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlfaA.00425.a.B1.GE36139	full length		1	443

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PF3D7_1357100
RefSeq:	XP_001350281.1
RefSeq:	XP_001350282.1
UniProt:	Q8I0P6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGKEKTHINL VVIGHVDSGK STTTGHIIYK LGGIDRRTIE KFEKESAEMG KGSFKYAWVL DKLKAERERG ITIDIALWKF ETPRYFFTVI DAPGHKDFIK NMITGTSQAD VALLVVPAEV GGFEGAFSKE GQTKEHALLA FTLGVKQIVV GVNKMDTVKY SEDRYEEIKK EVKDYLKKVG YQADKVDFIP ISGFEGDNLI EKSDKTPWYK GRTLIEALDT MEPPKRPYDK PLRIPLQGVY KIGGIGTVPV GRVETGILKA GMVLNFAPSA VVSECKSVEM HKEVLEEARP GDNIGFNVKN VSVKEIKRGY VASDTKNEPA KGCSKFTAQV IILNHPGEIK NGYTPVLDCH TSHISCKFLN IDSKIDKRSG KVVEENPKAI KSGDSALVSL EPKKPMVVET FTEYPPLGRF AIRDMRQTIA VGIIKSVEKK EPGAVTAKAP AKK

NT Sequence

atgggtaagg aaaaaacaca tattaactta gttgttattg gtcacgtcga tagtggaaaa tcaaccacta caggtcacat tatttacaaa ttaggaggta tagatagaag aactattgaa aaatttgaga aagaatctgc tgaaatgggt aagggtagtt ttaaatatgc atgggtttta gataaactta aggcagaaag agaaagaggt attaccattg atattgcctt atggaaattt gaaaccccaa ggtatttctt tactgtcatt gatgcaccag gtcacaagga ttttattaaa aatatgatta caggtacatc acaagcagat gttgctttat tagttgtccc agctgaagta ggtggtttcg aaggtgcctt ttcaaaggaa ggtcaaacaa aggaacacgc tttattagca tttaccttag gtgtaaaaca aattgttgtc ggtgttaaca agatggatac tgtcaaatat tctgaagaca gatatgaaga aattaaaaaa gaagttaagg attacttgaa gaaagtagga taccaagctg ataaagttga ttttattcca atttctggtt ttgaaggtga taatttaatc gaaaaatcag ataaaacacc atggtataaa ggaagaacct taatagaagc ccttgatact atggaaccac caaagagacc ttacgacaag ccattaagaa ttccacttca aggtgtttac aaaatcggtg gtattggtac cgtccctgtt ggtcgtgttg aaacaggtat cttaaaagcc ggtatggttt taaacttcgc tccatcagct gttgtgtctg aatgtaaatc agttgaaatg cacaaggaag ttttggaaga agctagacca ggagacaaca ttggttttaa cgtaaagaat gtttctgtta aagaaatcaa gagaggttac gttgcttcag atactaaaaa tgaaccagca aaaggatgct ccaaatttac tgctcaagtt attatattga accaccctgg tgaaatcaaa aatggttata ccccagtttt ggattgtcac acatctcaca tttcatgtaa atttttaaac atcgactcca agatcgacaa acgttcaggt aaagttgttg aagaaaaccc aaaagctatc aaatcaggag attcagcttt agttagttta gaacctaaga aacctatggt tgtcgaaacc tttactgaat atccaccatt aggaagattt gctattcgtg atatgagaca aaccattgct gtcggtatca ttaaatcagt tgaaaagaaa gaacctggag ctgtcacagc taaagcacca gccaaaaaat aa

Details for PlfaA.00425.a.B1.GE36139

HARVESTED ON:	9/26/2012
SEQUENCED ON:	1/7/2013
EXPECTED MW:	49kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG KEKTHINLVV IGHVDSGKST TTGHIIYKLG GIDRRTIEKF EKESAEMGKG SFKYAWVLDK LKAERERGIT IDIALWKFET PRYFFTVIDA PGHKDFIKNM ITGTSQADVA LLVVPAEVGG FEGAFSKEGQ TKEHALLAFT LGVKQIVVGV NKMDTVKYSE DRYEEIKKEV KDYLKKVGYQ ADKVDFIPIS GFEGDNLIEK SDKTPWYKGR TLIEALDTME PPKRPYDKPL RIPLQGVYKI GGIGTVPVGR VETGILKAGM VLNFAPSAVV SECKSVEMHK EVLEEARPGD NIGFNVKNVS VKEIKRGYVA SDTKNEPAKG CSKFTAQVII LNHPGEIKNG YTPVLDCHTS HISCKFLNID SKIDKRSGKV VEENPKAIKS GDSALVSLEP KKPMVVETFT EYPPLGRFAI RDMRQTIAVG IIKSVEKKEP GAVTAKAPAK K

Validated NT Sequence

atcttactca cttatttttt ggctggtgct ttagctgtga cagctccagg ttctttcttt tcaactgatt taatgatacc gacagcaatg gtttgtctca tatcacgaat agcaaatctt cctaatggtg gatattcagt aaaggtttcg acaaccatag gtttcttagg ttctaaacta actaaagctg aatctcctga tttgatagct tttgggtttt cttcaacaac tttacctgaa cgtttgtcga tcttggagtc gatgtttaaa aatttacatg aaatgtgaga tgtgtgacaa tccaaaactg gggtataacc atttttgatt tcaccagggt ggttcaatat aataacttga gcagtaaatt tggagcatcc ttttgctggt tcatttttag tatctgaagc aacgtaacct ctcttgattt ctttaacaga aacattcttt acgttaaaac caatgttgtc tcctggtcta gcttcttcca aaacttcctt gtgcatttca actgatttac attcagacac aacagctgat ggagcgaagt ttaaaaccat accggctttt aagatacctg tttcaacacg accaacaggg acggtaccaa taccaccgat tttgtaaaca ccttgaagtg gaattcttaa tggcttgtcg taaggtctct ttggtggttc catagtatca agggcttcta ttaaggttct tcctttatac catggtgttt tatctgattt ttcgattaaa ttatcacctt caaaaccaga aattggaata aaatcaactt tatcagcttg gtatcctact ttcttcaagt aatccttaac ttctttttta atttcttcat atctgtcttc agaatatttg acagtatcca tcttgttaac accgacaaca atttgtttta cacctaaggt aaatgctaat aaagcgtgtt cctttgtttg accttccttt gaaaaggcac cttcgaaacc acctacttca gctgggacaa ctaataaagc aacatctgct tgtgatgtac ctgtaatcat atttttaata aaatccttgt gacctggtgc atcaatgaca gtaaagaaat accttggggt ttcaaatttc cataaggcaa tatcaatggt aatacctctt tctctttctg ccttaagttt atctaaaacc catgcatatt taaaactacc cttacccatt tcagcagatt ctttctcaaa tttttcaata gttcttctat ctatacctcc taatttgtaa ataatgtgac ctgtagtggt tgattttcca ctatcgacgt gaccaataac aactaagtta atatgtgttt tttccttacc catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta aggaaaaaac acatattaac ttagttgtta ttggtcacgt cgatagtgga aaatcaacca ctacaggtca cattatttac aaattaggag gtatagatag aagaactatt gaaaaatttg agaaagaatc tgctgaaatg ggtaagggta gttttaaata tgcatgggtt ttagataaac ttaaggcaga aagagaaaga ggtattacca ttgatattgc cttatggaaa tttgaaaccc caaggtattt ctttactgtc attgatgcac caggtcacaa ggattttatt aaaaatatga ttacaggtac atcacaagca gatgttgctt tattagttgt cccagctgaa gtaggtggtt tcgaaggtgc cttttcaaag gaaggtcaaa caaaggaaca cgctttatta gcatttacct taggtgtaaa acaaattgtt gtcggtgtta acaagatgga tactgtcaaa tattctgaag acagatatga agaaattaaa aaagaagtta aggattactt gaagaaagta ggataccaag ctgataaagt tgattttatt ccaatttctg gttttgaagg tgataattta atcgaaaaat cagataaaac accatggtat aaaggaagaa ccttaataga agcccttgat actatggaac caccaaagag accttacgac aagccattaa gaattccact tcaaggtgtt tacaaaatcg gtggtattgg taccgtccct gttggtcgtg ttgaaacagg tatcttaaaa gccggtatgg ttttaaactt cgctccatca gctgttgtgt ctgaatgtaa atcagttgaa atgcacaagg aagttttgga agaagctaga ccaggagaca acattggttt taacgtaaag aatgtttctg ttaaagaaat caagagaggt tacgttgctt cagatactaa aaatgaacca gcaaaaggat gctccaaatt tactgctcaa gttattatat tgaaccaccc tggtgaaatc aaaaatggtt ataccccagt tttggattgt cacacatctc acatttcatg taaattttta aacatcgact ccaagatcga caaacgttca ggtaaagttg ttgaagaaaa cccaaaagct atcaaatcag gagattcagc tttagttagt ttagaaccta agaaacctat ggttgtcgaa acctttactg aatatccacc attaggaaga tttgctattc gtgatatgag acaaaccatt gctgtcggta tcattaaatc agttgaaaag aaagaacctg gagctgtcac agctaaagca ccagccaaaa aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat