PlfaA.00407.a

ADP-ribosylation factor, putative

CENTER ID: PlfaA.00407.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.00407.a.B4.GE36134 Crowther ProteinCCD 2 57 224
PlfaA.00407.a.B6.GE36136 Crowther XtalPred/ModBase 1 57 216
PlfaA.00407.a.B8.GE36138 Crowther FoldIndex 2 57 203
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1316200
RefSeq: XP_001349899.1
UniProt: Q8IEF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNVLDVLKRW FILVFFMLQR FFEFKSKITK KSFIIFGLPS SGKTSIIYFF KLGYLITTVS TLFINEENFK INLKIEKDDT KEQNYDITFY EVGKNCSYNL IKEYSDISND VIYIVDSVQK GNLSEARDDF IRILYEFRFI YRKCKFLIFM NKQDSNGCLS SQEIINFFAL PKDLLIRCNF ISCSTLSGQG LKEGLEWLLY YNLPFYNNEL NCIDRRTATK HLEL
NT Sequence
atgaatgttt tagatgtttt aaaaagatgg tttattcttg ttttttttat gttacaacgt ttttttgaat ttaaatcaaa aataaccaaa aaaagtttca ttatttttgg attgccttca tctggaaaaa catccataat atattttttc aagcttggat atttaataac gactgtaagt acccttttta ttaatgaaga gaatttcaag ataaatttaa aaattgaaaa agacgacacg aaggaacaaa attatgatat tacattttat gaagttggaa aaaattgttc ttataattta ataaaagaat attcagatat atctaatgat gttatatata ttgttgatag tgtacaaaaa ggaaacttaa gtgaagcaag agatgatttt atacgtattc tttatgaatt tagatttatt tatagaaaat gtaaatttct catttttatg aataagcaag attctaatgg atgtttgtca tcacaagaaa ttattaactt ttttgcttta ccaaaagatt tattaataag atgtaatttt atatcttgta gtactttatc cggtcaagga ttaaaagagg gcttagaatg gttactctat tataatctac ctttttataa taacgaatta aattgtattg atagaagaac agctactaaa catttggaat tataa
Details for PlfaA.00407.a.B4.GE36134
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 20kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT TVSTLFINEE NFKINLKIEK DDTKEQNYDI TFYEVGKNCS YNLIKEYSDI SNDVIYIVDS VQKGNLSEAR DDFIRILYEF RFIYRKCKFL IFMNKQDSNG CLSSQEIINF FALPKDLLIR CNFISCSTLS GQGLKEGLXW LLYYNLPFYN NELNCIDRRT ATKHLEL
Validated NT Sequence
ttnnnttnnc tttaagaagg aganatacca tggctcacca ccaccaccac catatgacga ctgtaagtac cctttttatt aatgaagaga atttcaagat aaatttaaaa attgaaaaag acgacacgaa ggaacaaaat tatgatatta cattttatga agttggaaaa aattgttctt ataatttaat aaaagaatat tcagatatat ctaatgatgt tatatatatt gttgatagtg tacaaaaagg aaacttaagt gaagcaagag atgattttat acgtattctt tatgaattta gatttattta tagaaaatgt aaatttctca tttttatgaa taagcaagat tctaatggat gtttgtcatc acaagaaatt attaactttt ttgctttacc aaaagattta ttaataagat gtaattttat atcttgtagt actttatccg gtcaaggatt aaaagagggc ttanaatggt tactctatta taatctacct ttttataata acgaattaaa ttgtattgat agaagaacag ctactaaaca tttggaatta taagtgagta agataggatc cggctgctac nnnccccnna angannnnnn ntnnnnnnnn nnnnnnaang acgtcnctgc ccggctgtat gcncgaggtt accgactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca acgcccataa ngcgggctgt tgcccggcat ccnacgccat tcatggncat atcaatgatt ttctggtgcg taccgggttg agaagcggtg taagtgaact gcagttgcca tgttttacgg cagtgananc anaatagcgc tgangtcnnn gnngcttttg ccgttacncn cacccntcag tagctganng gnggacagct gannnaancn gancnactgn ancnctcnaa anntcataca ctaaatcagn agnnnagcat cnccctanga ngtnnatatn tgtnnnngng nanaaannna nctnntnann annnatgatt tnnncgtntn ngannnnatt nntnnnnaaa nnnnnnnncn tttnnganan canatnnnnn nnnntnnnnn nnnnnnnttt nnntnngnnn ntnnnngnnn ngnnnnnnnn nnnnnnnccn ntnnnnnnnn tt
Expected Protein Sequence
MAHHHHHHMT TVSTLFINEE NFKINLKIEK DDTKEQNYDI TFYEVGKNCS YNLIKEYSDI SNDVIYIVDS VQKGNLSEAR DDFIRILYEF RFIYRKCKFL IFMNKQDSNG CLSSQEIINF FALPKDLLIR CNFISCSTLS GQGLKEGLEW LLYYNLPFYN NELNCIDRRT ATKHLEL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga ctgtaagtac cctttttatt aatgaagaga atttcaagat aaatttaaaa attgaaaaag acgacacgaa ggaacaaaat tatgatatta cattttatga agttggaaaa aattgttctt ataatttaat aaaagaatat tcagatatat ctaatgatgt tatatatatt gttgatagtg tacaaaaagg aaacttaagt gaagcaagag atgattttat acgtattctt tatgaattta gatttattta tagaaaatgt aaatttctca tttttatgaa taagcaagat tctaatggat gtttgtcatc acaagaaatt attaactttt ttgctttacc aaaagattta ttaataagat gtaattttat atcttgtagt actttatccg gtcaaggatt aaaagagggc ttagaatggt tactctatta taatctacct ttttataata acgaattaaa ttgtattgat agaagaacag ctactaaaca tttggaatta tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for PlfaA.00407.a.B6.GE36136
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 20kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT TVSTLFINEE NFKINLKIEK DDTKEQNYDI TFYEVGKNCS YNLIKEYSDI SNDVIYIVDS VQKGNLSEAR DDFIRILYEF RFIYRKCKFL IFMNKQDSNG CLSSQEIINF FALPKDLLIR CNFISCSTLS GQGLKEGLEW LLYYNLPFYN NELNCIDRR
Validated NT Sequence
ttttntnnnn tttaagaagg aganatacca tggctcacca ccaccaccac catatgacga ctgtaagtac cctttttatt aatgaagaga atttcaagat aaatttaaaa attgaaaaag acgacacgaa ggaacaaaat tatgatatta cattttatga agttggaaaa aattgttctt ataatttaat aaaagaatat tcagatatat ctaatgatgt tatatatatt gttgatagtg tacaaaaagg aaacttaagt gaagcaagag atgattttat acgtattctt tatgaattta gatttattta tagaaaatgt aaatttctca tttttatgaa taagcaagat tctaatggat gtttgtcatc acaagaaatt attaactttt ttgctttacc aaaagattta ttaataagat gtaattttat atcttgtagt actttatccg gtcaaggatt aaaagagggc ttagaatggt tactctatta taatctacct ttttataata acgaattaaa ttgtattgat agaagataag tgagtaagat aggatccggc tgctaacang nnnnnnnnnn nnnnnnnngn tncccccnnn nnnnnnnntn nnnnnnannn nnnnnnnnnn ntcanannnn ncnnnntggt cnnnncnnan tnnnnngcnn nnnannnang aanncacncn nnnnnncnnn annanntana nnnnnnnnnc nnaccnannt gtntnnnnan annnnnnaaa ncnccnnann nntnnnngnn nnnnnnaatt cnnnnnnnnn nantnnnnnt ncnnnnncnn nnnnnngnnn anantnnnaa cnnnannnaa nnnnnnctnn nnnannannn nnnncnangn nncnnnnnag nangnaacnn tannnnnnnc tgtnnnnnnn nnnnnanntn annnnannnn anannncnnn nnnggnnnnn nnnnnnttnn nnncnannnn nnntnnnnnn nnnntnnnng nnngnannnt nngannnnnc cnnnnnccnt tcctnnnann nntnnnnnnn ncc
Expected Protein Sequence
MAHHHHHHMT TVSTLFINEE NFKINLKIEK DDTKEQNYDI TFYEVGKNCS YNLIKEYSDI SNDVIYIVDS VQKGNLSEAR DDFIRILYEF RFIYRKCKFL IFMNKQDSNG CLSSQEIINF FALPKDLLIR CNFISCSTLS GQGLKEGLEW LLYYNLPFYN NELNCIDRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga ctgtaagtac cctttttatt aatgaagaga atttcaagat aaatttaaaa attgaaaaag acgacacgaa ggaacaaaat tatgatatta cattttatga agttggaaaa aattgttctt ataatttaat aaaagaatat tcagatatat ctaatgatgt tatatatatt gttgatagtg tacaaaaagg aaacttaagt gaagcaagag atgattttat acgtattctt tatgaattta gatttattta tagaaaatgt aaatttctca tttttatgaa taagcaagat tctaatggat gtttgtcatc acaagaaatt attaactttt ttgctttacc aaaagattta ttaataagat gtaattttat atcttgtagt actttatccg gtcaaggatt aaaagagggc ttagaatggt tactctatta taatctacct ttttataata acgaattaaa ttgtattgat agaagatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PlfaA.00407.a.B8.GE36138
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 18kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT TVSTLFINEE NFKINLKIEK DDTKEQNYDI TFYEVGKNCS YNLIKEYSDI SNDVIYIVDS VQKGNLSEAR DDFIRILYEF RFIYRKCKFL IFMNKQDSNG CLSSQEIINF FALPKDLLIR CNFISCSTLS GQGLKEGLEW LLYYNL
Validated NT Sequence
antttnnnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatgacg actgtaagta ccctttttat taatgaagag aatttcaaga taaatttaaa aattgaaaaa gacgacacga aggaacaaaa ttatgatatt acattttatg aagttggaaa aaattgttct tataatttaa taaaagaata ttcagatata tctaatgatg ttatatatat tgttgatagt gtacaaaaag gaaacttaag tgaagcaaga gatgatttta tacgtattct ttatgaattt agatttattt atagaaaatg taaatttctc atttttatga ataagcaaga ttctaatgga tgtttgtcat cacaagaaat tattaacttt tttgctttac caaaagattt attaataaga tgtaatttta tatcttgtag tactttatcc ggtcaaggat taaaagaggg cttagaatgg ttactctatt ataatctata agtgagtaag ataggatccg gctgctaaca gannnnnnnn nnnaaannnn nnnnntnnng gnnnncnnna nnanngnnng aanccaagcn nnnnnannnn nnnnnnnnnn nnnnnncnnn ntntnanntn nnnnnatnnn cnnannntna tannannnnn nnntatnnnn nnngnannnn nnnnnnnnnn ncnngannnn nnnnnaccnn nanannnnnn tanngnnnnc nttnnnnnnn tncnnannan nngnnnttnc nattcanaan tcncatnant nntngntnag tgntnnnnag tnnnnnnann acnannnann nnnnctnntc nnnnnngnnn cnnnnngnaa ctcnnnnnna ntnnncnnng annnnntnna gaannntnga cnagtnnnnn nntnnnnttn acnnnnnnnn ntncnnnnan nnnntcannn nnnnnnnnnn ncntnanann nnannnnanc gancnnnngn nnncnnnnnn tnnnnnnnnn nnnttnntnn gnannnnnnn nnnnnaannn nannnnngnn nnnnnacccn nnnnnnncnn nnnnnnnann nt
Expected Protein Sequence
MAHHHHHHMT TVSTLFINEE NFKINLKIEK DDTKEQNYDI TFYEVGKNCS YNLIKEYSDI SNDVIYIVDS VQKGNLSEAR DDFIRILYEF RFIYRKCKFL IFMNKQDSNG CLSSQEIINF FALPKDLLIR CNFISCSTLS GQGLKEGLEW LLYYNL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga ctgtaagtac cctttttatt aatgaagaga atttcaagat aaatttaaaa attgaaaaag acgacacgaa ggaacaaaat tatgatatta cattttatga agttggaaaa aattgttctt ataatttaat aaaagaatat tcagatatat ctaatgatgt tatatatatt gttgatagtg tacaaaaagg aaacttaagt gaagcaagag atgattttat acgtattctt tatgaattta gatttattta tagaaaatgt aaatttctca tttttatgaa taagcaagat tctaatggat gtttgtcatc acaagaaatt attaactttt ttgctttacc aaaagattta ttaataagat gtaattttat atcttgtagt actttatccg gtcaaggatt aaaagagggc ttagaatggt tactctatta taatctatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at