PlfaA.00179.a

Orotate phosphoribosyltransferase (EC 2.4.2.10)

CENTER ID: PlfaA.00179.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.00179.a.B4.GE36127 Crowther ModBase/XtalPred 79 281
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_0512700
RefSeq: XP_001351684.1
UniProt: Q8I3Y0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTIKENEFL CDEEIYKSFV HLKDKICEER KKKELVNNNI DNVNFNDDDD NNYDDDGNSY SSYIKEMKKL LKVVLLKYKA LKFGEFILKS KRKSNYFFSS GVLNNIVSSN IICFLLSELI LKNKLSFDYL LGASYKGIPM VSLTSHFLFE SKKYSNIFYL YDRKEKKEYG DKNVIVGNLD DDDKDILNLK KKTKNNQDEE KKNIIIIDDV FTCGTALTEI LAKLKTYEHL KVVAFIVLLN RNEYEINENN QKIYFKDIFE KRVGIPLYSI LSYKDDIQSM I
NT Sequence
atgacgacga taaaagagaa tgaatttttg tgtgatgagg agatatataa aagttttgta catctgaagg ataagatatg tgaggaaaga aaaaagaagg aacttgttaa taataatatt gataatgtta attttaatga tgatgatgat aataattatg atgatgatgg taattcttat agttcctaca ttaaagagat gaagaaatta ttaaaagttg ttcttttaaa atataaggca ttaaaatttg gagaatttat tttaaaatcg aaaagaaaat caaactattt tttttcaagt ggagtattaa ataatattgt ttcttcaaat ataatttgtt ttttattatc cgaattaata ttaaaaaaca aattatcatt tgattattta ttaggtgctt catataaagg tattcctatg gtatccttaa caagtcactt tttatttgaa tccaaaaaat attctaacat tttttattta tatgatagaa aagaaaaaaa agaatatggt gataaaaatg ttattgtggg aaatcttgat gatgatgata aagatatact aaacttaaaa aaaaaaacaa aaaataatca agatgaagaa aagaaaaata tcataattat tgatgatgtt tttacttgtg gaacagcatt aacggaaata ttagccaaat taaaaacata tgaacattta aaagtagtag cgtttatagt attgcttaat agaaatgaat atgagataaa cgaaaataat caaaagatat attttaagga tatctttgag aaaagggtag gaatacctct ctacagtata ttatcttaca aagacgatat acagtcgatg atatga
Details for PlfaA.00179.a.B4.GE36127
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK ALKFGEFILK SKRKSNYFFS XXVLNNIVSS NIICFLLSEL ILKNKLSFDY LLGASYKGIP MVSLTSHFLF ESKKYSNIFY LYDRKEKKEY GDKNVIVGNL DDDDKDILNL KKKTKNNQDE EKKNIIIIDD VFTCGTALTE ILAKLKTYEH LKVVAFIVLL NRNEYEINEN NQKIYFKDIF EKRVGIPLYS ILSYKDDIQS MI
Validated NT Sequence
ttanctttaa gaaggagana taccatggct caccaccacc accaccatat gaaggcatta aaatttggag aatttatttt aaaatcgaaa agaaaatcaa actatttttt ttcnnnngna gtattaaata atattgtttc ttcaaatata atttgttttt tattatccga attaatatta aaaaacaaat tatcatttga ttatttatta ggtgcttcat ataaaggtat tcctatggta tccttaacaa gtcacttttt atttgaatcc aaaaaatatt ctaacatttt ttatttatat gatagaaaag aaaaaaaaga atatggtgat aaaaatgtta ttgtgggaaa tcttgatgat gatgataaag atatactaaa cttaaaaaaa aaaacaaaaa ataatcaaga tgaagaaaag aaaaatatca taattattga tgatgttttt acttgtggaa cagcattaac ggaaatatta gccaaattaa aaacatatga acatttaaaa gtagtagcgt ttatagtatt gcttaataga aatgaatatg agataaacga aaataatcaa aagatatatt ttaaggatat ctttgagaaa agggtaggaa tacctctcta cagtatatta tcttacaaag acgatataca gtcgatgata taagtgagta aganaggatc cggnngnnnc cccnnnnnnn nnnnnnnnnn nnnnnanaaa aaaaaaaann nnnagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc nngaattnnn nnnnnacnng nnttnnnnnn anncnnnncn nnnntnnnnn ncnnnnctnn nnnnnannnn nnnnnncncn nnnncnnnnn ntnncnnnnn naacnnnnnn nnnnntntcn nnnnnnnnnn gcnnnannnn annncantnn natncttcnn annnacccnn nnnnnnnnnn cnnnnnaaan nnnnnncnnc annnnnnnnn nccnnanaan nnnnnantan annnncnnnn nnnncnncnn nnnnnnngnn annngtnnnn nnnnnannnn nnnngnnnan nnnnntngnn nnnnnannnn nnntnnnann nannnnnaa
Expected Protein Sequence
MAHHHHHHMK ALKFGEFILK SKRKSNYFFS SGVLNNIVSS NIICFLLSEL ILKNKLSFDY LLGASYKGIP MVSLTSHFLF ESKKYSNIFY LYDRKEKKEY GDKNVIVGNL DDDDKDILNL KKKTKNNQDE EKKNIIIIDD VFTCGTALTE ILAKLKTYEH LKVVAFIVLL NRNEYEINEN NQKIYFKDIF EKRVGIPLYS ILSYKDDIQS MI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagg cattaaaatt tggagaattt attttaaaat cgaaaagaaa atcaaactat tttttttcaa gtggagtatt aaataatatt gtttcttcaa atataatttg ttttttatta tccgaattaa tattaaaaaa caaattatca tttgattatt tattaggtgc ttcatataaa ggtattccta tggtatcctt aacaagtcac tttttatttg aatccaaaaa atattctaac attttttatt tatatgatag aaaagaaaaa aaagaatatg gtgataaaaa tgttattgtg ggaaatcttg atgatgatga taaagatata ctaaacttaa aaaaaaaaac aaaaaataat caagatgaag aaaagaaaaa tatcataatt attgatgatg tttttacttg tggaacagca ttaacggaaa tattagccaa attaaaaaca tatgaacatt taaaagtagt agcgtttata gtattgctta atagaaatga atatgagata aacgaaaata atcaaaagat atattttaag gatatctttg agaaaagggt aggaatacct ctctacagta tattatctta caaagacgat atacagtcga tgatatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat