PlfaA.00043.a

Dihydroorotase, putative

CENTER ID: PlfaA.00043.a
ORGANISM: Plasmodium falciparum 3D7
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlfaA.00043.a.B2.GE36124 Crowther ProteinCCD 1 352
PlfaA.00043.a.B3.GE36125 Crowther XtalPred/HHpred/ModBase 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PF3D7_1472900
RefSeq: XP_001348871.1
UniProt: Q8IKA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNYFYIPIA DDMHCHLRQG DMLDFTVNSI RRGGCNRVLV MPNTHPIIST CSDAQKYLYQ LKSRDDDIEY LMTLYLNKNT DENDILSNYY KCNLQGVKIY PSNVTTNSSD GITSLEPYYK VFHALEKLNK SIHIHCEEPN INPLYAEEKY LPHIHDLAIK FPGLNIVLEH ISSSESINVI KEFRNVAGSI TPHHLYLTID DVVNMDIYDH AIDNTYIEKY IKNTYHYCKP LPKLLEDKIA LQDVIKDDFP RVFLGSDSAP HYKVMKRKPY YKPGIYTQPF LINYVAHILN KFDALDKMEN FTSKNASLFL NLAEKKKLAK YYICVEKHPF KLPREYNGVV PFLAGKTLDY DIHYVSKF
NT Sequence
atgaaaaatt acttttatat tccaatagct gatgacatgc actgtcactt aaggcaaggc gacatgctgg attttacggt aaattccatt agaagaggcg gctgtaatcg tgttttagtg atgccgaaca cgcatcctat tataagtact tgtagtgatg cacaaaaata tttatatcaa ctgaaaagtc gtgatgatga tatagaatat ttaatgacct tgtatttgaa taagaatacg gatgaaaacg atatattaag taattactat aagtgtaatt tacaaggcgt aaaaatatat cctagtaatg taaccactaa ttcgagtgat ggtattacta gcctagaacc ttattataag gtatttcatg cactagaaaa attaaataag agtatacata tccattgtga ggaaccaaat ataaatccat tatatgctga ggaaaagtat ttaccgcata tacatgattt agccataaag tttccaggtt tgaatattgt tttagaacat atatcgtcaa gcgaatcgat aaatgttatt aaagaatttc gaaatgtagc aggttccata acacctcatc atttatattt aacaatagat gatgttgtta atatggatat atatgatcat gcaatagata acacatatat tgaaaaatat ataaaaaata catatcatta ttgtaagcca ttaccaaaat tgttagaaga taaaattgct ttacaagatg ttataaaaga tgattttcca agagtctttt tgggttcgga ttcagcacct cattacaaag ttatgaagcg caaaccctac tataaaccag gaatatacac acaaccattt ttaataaatt atgttgctca tatattgaac aaattcgatg ctttagataa gatggaaaat tttacctcaa aaaatgcttc cctctttcta aatttagcag aaaaaaaaaa attggcaaaa tattacatat gtgtggaaaa acatccattt aaattaccaa gagaatataa tggtgtagtc ccctttttgg cagggaagac tttggattat gacatacatt atgtaagtaa gttttaa
Details for PlfaA.00043.a.B2.GE36124
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 60
Validated AA Sequence
MAHHHHHHMK NYFYIPIADD MHCHLRQGDM LDFTVNSIRR GGCNRVLVMP NTHPIISTCS DAQKYLYQLK SRDDDIEYLM TLYLNKNTDE NDILSNYYKC NLQGVKIYPS NVTTNSSDGI TSLEPYYKVF HALEKLNKSI HIHCEEPNIN PLYAEEKYLP HIHDLAIKFP GLNIVLEHIS SSESINVIKE FRNVAGSITP HHLYLTIDDV VNMGYI
Validated NT Sequence
annnnnttnn nttnacttta agaaggagan ataccatggc tcaccaccac caccaccata tgaaaaatta cttttatatt ccaatagctg atgacatgca ctgtcactta aggcaaggcg acatgctgga ttttacggta aattccatta gaagaggcgg ctgtaatcgt gttttagtga tgccgaacac gcatcctatt ataagtactt gtagtgatgc acaaaaatat ttatatcaac tgaaaagtcg tgatgatgat atagaatatt taatgacctt gtatttgaat aagaatacgg atgaaaacga tatattaagt aattactata agtgtaattt acaaggcgta aaaatatatc ctagtaatgt aaccactaat tcgagtgatg gtattactag cctagaacct tattataagg tatttcatgc actagaaaaa ttaaataaga gtatacatat ccattgtgag gaaccaaata taaatccatt atatgctgag gaaaagtatt taccgcatat acatgattta gccataaagt ttccaggttt gaatattgtt ttagaacata tatcgtcaag cgaatcgata aatgttatta aagaatttcg aaatgtagca ggttccataa cacctcatca tttatattta acaatagatg atgttgttaa tatgggatat atatgatcat gcaatagata acacatatat tgaaaaatat ataaaaaata catatcatta ttgtaagcca ttaccaaaat tgttagaaga taaaattgct ttacaagatg ttataaaaga tgantttcca agagtctttt tgggttcgga ttcagcacct cattacaaag ttatgaagcg caaaccctac tataaaccnn natatacaca caaccatttt taataaatta tgttgctcat atatngaaca aattcgatgc tttagataaa tggaaaattt tacctcaaaa aatgcttccc tctttctaaa tttagcnnaa aaaaaaaaat tggcaaannn tannnntgnn nggaaaaacn tccntttnaa ttaccaannn aannnnangn ngnantcccc ntttngcngg nanannttna tnnngannnn annnnnnnna aannnnnnnn aaaaaaa
Expected Protein Sequence
MAHHHHHHMK NYFYIPIADD MHCHLRQGDM LDFTVNSIRR GGCNRVLVMP NTHPIISTCS DAQKYLYQLK SRDDDIEYLM TLYLNKNTDE NDILSNYYKC NLQGVKIYPS NVTTNSSDGI TSLEPYYKVF HALEKLNKSI HIHCEEPNIN PLYAEEKYLP HIHDLAIKFP GLNIVLEHIS SSESINVIKE FRNVAGSITP HHLYLTIDDV VNMDIYDHAI DNTYIEKYIK NTYHYCKPLP KLLEDKIALQ DVIKDDFPRV FLGSDSAPHY KVMKRKPYYK PGIYTQPFLI NYVAHILNKF DALDKMENFT SKNASLFLNL AEKKKLAKYY ICVEKHPFKL PREYNGVVPF LAGKTLDYDI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa attactttta tattccaata gctgatgaca tgcactgtca cttaaggcaa ggcgacatgc tggattttac ggtaaattcc attagaagag gcggctgtaa tcgtgtttta gtgatgccga acacgcatcc tattataagt acttgtagtg atgcacaaaa atatttatat caactgaaaa gtcgtgatga tgatatagaa tatttaatga ccttgtattt gaataagaat acggatgaaa acgatatatt aagtaattac tataagtgta atttacaagg cgtaaaaata tatcctagta atgtaaccac taattcgagt gatggtatta ctagcctaga accttattat aaggtatttc atgcactaga aaaattaaat aagagtatac atatccattg tgaggaacca aatataaatc cattatatgc tgaggaaaag tatttaccgc atatacatga tttagccata aagtttccag gtttgaatat tgttttagaa catatatcgt caagcgaatc gataaatgtt attaaagaat ttcgaaatgt agcaggttcc ataacacctc atcatttata tttaacaata gatgatgttg ttaatatgga tatatatgat catgcaatag ataacacata tattgaaaaa tatataaaaa atacatatca ttattgtaag ccattaccaa aattgttaga agataaaatt gctttacaag atgttataaa agatgatttt ccaagagtct ttttgggttc ggattcagca cctcattaca aagttatgaa gcgcaaaccc tactataaac caggaatata cacacaacca tttttaataa attatgttgc tcatatattg aacaaattcg atgctttaga taagatggaa aattttacct caaaaaatgc ttccctcttt ctaaatttag cagaaaaaaa aaaattggca aaatattaca tatgtgtgga aaaacatcca tttaaattac caagagaata taatggtgta gtcccctttt tggcagggaa gactttggat tatgacatat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for PlfaA.00043.a.B3.GE36125
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMK NYFYIPIADD MHCHLRQGDM LDFTVNSIRR GGCNRVLVMP NTHPIISTCS DAQKYLYQLK SRDDDIEYLM TLYLNKNTDE NDILSNYYKC NLQGVKIYPS NVTTNSSDGI TSLEPYYKVF HALEKLNKSI HIHCEEPNIN PLYAEEKYLP HIHDLAIKFP GLNIVLEHIS SSESINVIKE FRNVAXSITP HHLYLTIDDV VNMDIYDHAI DNTYIEKYIK NTYHYCKPLP KLXEDKIALQ DVIKDXFPRV FLGSXSAPHX KVMKRKPYYK PXYTHNHF
Validated NT Sequence
ttnnnttant ttaagaagga gannnaccat ggctcaccac caccaccacc atatgaaaaa ttacttttat attccaatag ctgatgacat gcactgtcac ttaaggcaag gcgacatgct ggattttacg gtaaattcca ttagaagagg cggctgtaat cgtgttttag tgatgccgaa cacgcatcct attataagta cttgtagtga tgcacaaaaa tatttatatc aactgaaaag tcgtgatgat gatatagaat atttaatgac cttgtatttg aataagaata cggatgaaaa cgatatatta agtaattact ataagtgtaa tttacaaggc gtaaaaatat atcctagtaa tgtaaccact aattcgagtg atggtattac tagcctagaa ccttattata aggtatttca tgcactagaa aaattaaata agagtataca tatccattgt gaggaaccaa atataaatcc attatatgct gaggaaaagt atttaccgca tatacatgat ttagccataa agtttccagg tttgaatatt gttttagaac atatatcgtc aagcgaatcg ataaatgtta ttaaagaatt tcgaaatgta gcangttcca taacacctca tcatttatat ttaacaatag atgatgttgt taatatggat atatatgatc atgcaataga taacacatat attgaaaaat atataaaaaa tacatatcat tattgtaagc cattaccaaa attgntagaa gataaaattg ctttacaaga tgttataaaa gatgantttc caagagtctt tttgggttcg gantcagcac ctcatnacaa agttatgaag cgcaaaccct actataaacc nnnatataca cacaaccatt tttaataaat tatgntgctc atatnntgaa caaattcgan gctttanaan anggaaaatt ttacntcnaa aangcttccn tcnttctaan tttagcaaaa tantgannnn nnnnnnnnnn nnnnncnnna aaaannnaan nannaaanna aaannnnnnn nnnnnnannn nnnnnnnnnn nc
Expected Protein Sequence
MAHHHHHHMK NYFYIPIADD MHCHLRQGDM LDFTVNSIRR GGCNRVLVMP NTHPIISTCS DAQKYLYQLK SRDDDIEYLM TLYLNKNTDE NDILSNYYKC NLQGVKIYPS NVTTNSSDGI TSLEPYYKVF HALEKLNKSI HIHCEEPNIN PLYAEEKYLP HIHDLAIKFP GLNIVLEHIS SSESINVIKE FRNVAGSITP HHLYLTIDDV VNMDIYDHAI DNTYIEKYIK NTYHYCKPLP KLLEDKIALQ DVIKDDFPRV FLGSDSAPHY KVMKRKPYYK PGIYTQPFLI NYVAHILNKF DALDKMENFT SKNASLFLNL AEK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa attactttta tattccaata gctgatgaca tgcactgtca cttaaggcaa ggcgacatgc tggattttac ggtaaattcc attagaagag gcggctgtaa tcgtgtttta gtgatgccga acacgcatcc tattataagt acttgtagtg atgcacaaaa atatttatat caactgaaaa gtcgtgatga tgatatagaa tatttaatga ccttgtattt gaataagaat acggatgaaa acgatatatt aagtaattac tataagtgta atttacaagg cgtaaaaata tatcctagta atgtaaccac taattcgagt gatggtatta ctagcctaga accttattat aaggtatttc atgcactaga aaaattaaat aagagtatac atatccattg tgaggaacca aatataaatc cattatatgc tgaggaaaag tatttaccgc atatacatga tttagccata aagtttccag gtttgaatat tgttttagaa catatatcgt caagcgaatc gataaatgtt attaaagaat ttcgaaatgt agcaggttcc ataacacctc atcatttata tttaacaata gatgatgttg ttaatatgga tatatatgat catgcaatag ataacacata tattgaaaaa tatataaaaa atacatatca ttattgtaag ccattaccaa aattgttaga agataaaatt gctttacaag atgttataaa agatgatttt ccaagagtct ttttgggttc ggattcagca cctcattaca aagttatgaa gcgcaaaccc tactataaac caggaatata cacacaacca tttttaataa attatgttgc tcatatattg aacaaattcg atgctttaga taagatggaa aattttacct caaaaaatgc ttccctcttt ctaaatttag cagaaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat