SSGCID: Target Page

PlbeA.18308.a

Aspartyl (Acid) protease, putative

CENTER ID:	PlbeA.18308.a
ORGANISM:	Plasmodium berghei Anka
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlbeA.18308.a.B2.GE35067	aligned to Rhizopus		35	455

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PBANKA_133870
RefSeq:	XP_677725.1
UniProt:	Q4YSZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCFSKLYNFI TYFIIINILV QANTENEDIL NKNSEKNEEI YKYKLYGDID EYAYYFMDIN IGTPGQKLSL IVDTGSSSLS FPCSECKDCG VHMENPFNLN NSSTSSILYC NDNICPYNLK CVKGRCEYLQ SYCEGSRING FYFSDIVRLE SNNNTKNGNI TFKKHMGCHM HEEGLFLHQH ATGVLGLSLT KPKGVPTFID LLFKSSPKLN KIFSLCISEY GGELILGGYS KDYIVKEVSI DEKKDNIEHN KNENINSINK SIVDGILWEA ITRKYYYYIR VKGFQLFGTT FSHNNKSMEM LVDSGSTFTH LPDDLYNNLN FFFDILCIHN MNNPIDIEKK LKITNETLSN HLLYFDDFKS TLKNIISSEN VCVKIADNVQ CWRYLENLPN IYIKLSNNTK LVWQPSSYLY KKESFWCKGL EKQVNDKPIL GLSFFKNKQI IFDLKNNKIG FIESNCPSNP INTRPRTFNE YNIKENHLFK QSYFSLYAFS IIIALTFILY IILYIKKFIC SYYNPLHG

NT Sequence

atgtgttttt ctaaattata taattttatt acttatttta taataataaa tattttagtt caagcaaata ctgaaaatga agatatattg aataaaaata gcgaaaaaaa tgaggaaata tacaaatata aattatatgg agatattgat gaatatgcat attattttat ggatataaat ataggaacac ctgggcaaaa actttcacta atagtagaca ctggatcgtc ttcgctaagt ttcccatgtt cagaatgtaa agattgtggg gtgcatatgg aaaatccttt taatttaaat aattcatcaa cgtcatcgat tttatattgt aatgataata tttgcccata taatttaaaa tgtgtaaaag gaagatgtga atatttacaa tcttattgtg agggatctcg aattaacggt ttttattttt cggatattgt aaggctcgaa tcgaacaata atactaaaaa tggaaacata acttttaaga aacatatggg atgtcatatg catgaagaag ggttattttt acatcaacat gcgacgggag tattggggtt aagtttaaca aaaccaaaag gtgtaccgac ttttattgat ttattattta aaagttcccc taaattaaat aagatatttt cattgtgtat ttctgaatat ggaggtgaac taattttagg gggatatagc aaagactata ttgtcaaaga agtttcaatt gatgaaaaga aagataatat tgaacataat aagaatgaaa atattaattc gattaataaa tcgattgtag atggtatact ttgggaagct ataactagaa aatattatta ttatataaga gtcaaaggat ttcaattatt tggtacaact ttttctcata ataacaaaag tatggaaatg ctagtagatt ctggtagtac ttttacacat ctacctgatg atttatataa taatttgaat tttttttttg atatattatg tatacacaat atgaacaatc ctattgatat agagaaaaaa ttaaaaataa caaatgaaac gctaagcaac catcttttat attttgatga ttttaaatct acactaaaaa atataataag tagtgaaaat gtgtgtgtaa aaatagctga caatgtacaa tgttggagat atttagaaaa tttaccaaat atatatatta aactatctaa taatactaaa ctagtatggc aacctagttc ttatttatat aaaaaagaat cattttggtg taaaggacta gaaaaacaag taaatgacaa acctattctt ggattgagtt tttttaaaaa taaacaaatt atatttgatc ttaaaaataa taaaattggg tttattgaat caaattgtcc atctaatccg attaatacaa gaccgagaac ttttaatgag tataatataa aagaaaatca tttattcaaa caatcatatt ttagtttata tgccttttcc ataattattg ccttaacatt tatactttac attatcttat acattaaaaa gtttatatgt tcctattaca acccattgca tggataa

Details for PlbeA.18308.a.B2.GE35067

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHME KNEEIYKYKL YGDIDEYAYY FMDINIGTPG QKLSLIVDTG SSSLSFPCSE CKDCGVHMEN PFNLNNSSTS SILYCNDNIC PYNLKCVKGR CEYLQSYCEG SRINGFYFSD IVRLESNNNT KNGNITFKKH MGCHMHEEGL FLHQHATGVL GLSLTKPKGV PTFIDLLFKS SPKLNKIFSL CISEYGGELI LGGYSKDYIV KEVSIDEKKD NIEHNKNENI NSINKSIVDG ILWEAITRKY YYYIRVKGFQ LFGTTFSHNN KSMEMLVDSG STFTHLPDDL YNNLNFFFDI LCIHNMNNPI DIEKKLKITN ETLSNHLLYF DDFKSTLKNI ISSENVCVKI ADNVQCWRYL ENLPNIYIKL SNNTKLVWQP SSYLYKKESF WCKGLEKQVN DKPILGLSFF KNKQIIFDLK NNKIGFIESN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aaaatgagga aatatacaaa tataaattat atggagatat tgatgaatat gcatattatt ttatggatat aaatatagga acacctgggc aaaaactttc actaatagta gacactggat cgtcttcgct aagtttccca tgttcagaat gtaaagattg tggggtgcat atggaaaatc cttttaattt aaataattca tcaacgtcat cgattttata ttgtaatgat aatatttgcc catataattt aaaatgtgta aaaggaagat gtgaatattt acaatcttat tgtgagggat ctcgaattaa cggtttttat ttttcggata ttgtaaggct cgaatcgaac aataatacta aaaatggaaa cataactttt aagaaacata tgggatgtca tatgcatgaa gaagggttat ttttacatca acatgcgacg ggagtattgg ggttaagttt aacaaaacca aaaggtgtac cgacttttat tgatttatta tttaaaagtt cccctaaatt aaataagata ttttcattgt gtatttctga atatggaggt gaactaattt tagggggata tagcaaagac tatattgtca aagaagtttc aattgatgaa aagaaagata atattgaaca taataagaat gaaaatatta attcgattaa taaatcgatt gtagatggta tactttggga agctataact agaaaatatt attattatat aagagtcaaa ggatttcaat tatttggtac aactttttct cataataaca aaagtatgga aatgctagta gattctggta gtacttttac acatctacct gatgatttat ataataattt gaattttttt tttgatatat tatgtataca caatatgaac aatcctattg atatagagaa aaaattaaaa ataacaaatg aaacgctaag caaccatctt ttatattttg atgattttaa atctacacta aaaaatataa taagtagtga aaatgtgtgt gtaaaaatag ctgacaatgt acaatgttgg agatatttag aaaatttacc aaatatatat attaaactat ctaataatac taaactagta tggcaaccta gttcttattt atataaaaaa gaatcatttt ggtgtaaagg actagaaaaa caagtaaatg acaaacctat tcttggattg agttttttta aaaataaaca aattatattt gatcttaaaa ataataaaat tgggtttatt gaatcaaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat