SSGCID: Target Page

PlbeA.01010.a

Calmodulin-domain protein kinase, putative

CENTER ID:	PlbeA.01010.a
ORGANISM:	Plasmodium berghei Anka
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlbeA.01010.a.B1.GE35066	full length		1	528

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PBANKA_061520
RefSeq:	XP_676632.1
UniProt:	Q4Z5I1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGQEMSTQSD MQNENQKGNK RNLKGSQGKN GLKERSTSIS KEIVKNSFNN SKLRPGMFIQ NSNVVFNEQY KGIKILGKGS FGEVILSKDK HTGHEYAIKV ISKKHVKRKT DKQSLLREVE LLKMLDHINI MKLYEFFEDN NYYYLVSDVY SGGELFDEII SRKRFYEVDA ARIIKQVLSG ITYMHKNNVV HRDLKPENIL LETKNKEDMI IKIIDFGLST HFEYSKKMKD KIGTAYYIAP DVLHGTYDEK CDIWSCGVIL YILLSGCPPF NGSNEYDILK KVETGKYTFD LPQFKKISDK AKDLIKKMLM YTSAVRISAR DALEHEWIRL MTSKDNVNID IPSLELSITN IKQFQSTQKL AQAALLYMGS KLTTIDETKE LTKIFKKMDK NGDGQLDRNE LIIGYKELLK LKGDDTTDLD NAAIEVEVDQ ILSSIDLDQN GYIEYSEFLT VAIDRKLLLS TERLEKAFKL FDKDGSGKIS ANELAQLFGL GDVSSDCWKT VLKEVDQNND GEIDFKEFRD MLIKLCNY

NT Sequence

atggggcaag aaatgtctac acagagtgat atgcaaaatg aaaaccaaaa gggaaacaaa agaaatttaa aggggtccca aggaaaaaat ggtttgaaag aaagaagtac tagtatatca aaagaaatag ttaaaaatag ttttaataat agtaaattaa gacctggtat gttcatacaa aatagtaatg tagtatttaa tgagcaatat aaaggaatca agattttagg aaagggttca tttggtgaag ttatattaag taaagataaa catacaggtc atgaatatgc aattaaagta attagcaaaa aacatgttaa aagaaaaaca gataaacaaa gtcttttaag agaagtagaa ttattaaaaa tgttagatca tataaatata atgaaattat atgaattttt tgaagataat aattattatt atttagtgtc tgatgtatat tctggaggtg aattatttga tgaaattatt agtaggaaac gattttatga agtagatgca gctagaataa ttaaacaagt attaagtggt attacatata tgcataaaaa taatgtagtt catagagatt taaaacctga aaatattctt ctcgaaacaa aaaataaaga agatatgatt ataaaaatta ttgattttgg attatcaacc cattttgaat atagtaaaaa aatgaaagat aaaataggca ctgcctatta tattgctccg gatgttttac acggaactta tgatgaaaaa tgtgatatat ggtcatgtgg tgtcatatta tatattctct tatcaggatg tccacctttt aatggatcta atgaatatga cattttgaaa aaagttgaaa ctggaaaata tacttttgat ttacctcaat ttaaaaaaat aagtgacaaa gcgaaagact tgataaaaaa aatgttgatg tacacaagtg ctgtgagaat atcagcaaga gatgcattag aacatgaatg gataagatta atgacaagca aagataatgt aaacattgat attccatctc ttgaattaag tattacaaat ataaaacaat ttcaaagtac acaaaagtta gcacaagccg cactattata tatgggatca aaattaacaa caatagatga aacaaaagaa cttactaaaa tttttaaaaa aatggacaaa aatggggatg gtcaattaga cagaaatgaa ttaataatag gatataaaga attgttaaaa ttaaaaggag atgatacaac tgatttagat aatgcagcta ttgaagttga agtcgatcaa atattaagtt ctatagattt agatcaaaat ggatatatag aatattcaga atttttaaca gttgctattg atagaaaatt attattatca actgaaagat tagaaaaggc atttaaatta tttgataaag atggttcagg aaaaatatct gcaaatgaat tagctcaatt gtttggattg ggagatgtta gttctgattg ttggaaaact gttttgaaag aggttgatca aaataatgat ggggaaattg attttaaaga atttagagac atgttaataa aactatgtaa ctattaa

Details for PlbeA.01010.a.B1.GE35066

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/11/2012
EXPECTED MW:	61kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	52

Validated AA Sequence

Validated NT Sequence

tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggc aagaaatgtc tacacagagt gatatgcaaa atgaaaacca aaagggaaac aaaagaaatt taaaggggtc ccaaggaaaa aatggtttga aagaaagaag tactagtata tcaaaagaaa tagttaaaaa tagttttaat aatagtaaat taagacctgg tatgttcata caaaatagta atgtagtatt taatgagcaa tataaaggaa tcaagatttt aggaaagggt tcatttggtg aagttatatt aagtaaagat aaacatacag gtcatgaata tgcaattaaa gtaattagca aaaaacatgt taaaagaaaa acagataaac aaagtctttt aagagaagta gaattattaa aaatgttaga tcatataaat ataatgaaat tatatgaatt ttttgaagat aataattatt attatttagt gtctgatgta tattctggag gtgaattatt tgatgaaatt attagtagga aacgatttta tgaagtagat gcagctagaa taattaaaca agtattaagt ggtattacat atatgcataa aaataatgta gttcatagag atttaaaacc tgaaaatatt cttctcgaaa caaaaaataa agaagatatg attataaaaa ttattgattt tggattatca acccattttg aatatagtaa aaaaatgaaa gataaaatag gcactgccta ttatattgct ccggatgttt tacacggaac ttatgatgaa aaatgtgata tatggtcatg tggtgtcata ttatatattc tcttatcagg tataaaattt tcaaaataat tttgttataa attatagcat cgattttttt ttgcttaatt aatatgaaaa agaatgtata aaaatgtttt tttaaataat tctaaaaatt taaatgtcaa ttcnttgtat gcattgngtg tgatcctgtt natatatttt tttcnttttn atnggangtc caccntttnn gnnnctaatg annatgacat tttnnanann nnnnanaann tactttngan ttacctcnnt naaaaannan nnnnnnancn annatgnnaa aaaannnnnn ngtnnnnnnn ncnnnnnnan ntnnnnnnnn ncntnnnnnn nnnnnanntn nnnnnnnnnn nangnnnnnn nnnnntnctn nnnnnnnnnn nt

Expected Protein Sequence

MAHHHHHHMG QEMSTQSDMQ NENQKGNKRN LKGSQGKNGL KERSTSISKE IVKNSFNNSK LRPGMFIQNS NVVFNEQYKG IKILGKGSFG EVILSKDKHT GHEYAIKVIS KKHVKRKTDK QSLLREVELL KMLDHINIMK LYEFFEDNNY YYLVSDVYSG GELFDEIISR KRFYEVDAAR IIKQVLSGIT YMHKNNVVHR DLKPENILLE TKNKEDMIIK IIDFGLSTHF EYSKKMKDKI GTAYYIAPDV LHGTYDEKCD IWSCGVILYI LLSGCPPFNG SNEYDILKKV ETGKYTFDLP QFKKISDKAK DLIKKMLMYT SAVRISARDA LEHEWIRLMT SKDNVNIDIP SLELSITNIK QFQSTQKLAQ AALLYMGSKL TTIDETKELT KIFKKMDKNG DGQLDRNELI IGYKELLKLK GDDTTDLDNA AIEVEVDQIL SSIDLDQNGY IEYSEFLTVA IDRKLLLSTE RLEKAFKLFD KDGSGKISAN ELAQLFGLGD VSSDCWKTVL KEVDQNNDGE IDFKEFRDML IKLCNY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggc aagaaatgtc tacacagagt gatatgcaaa atgaaaacca aaagggaaac aaaagaaatt taaaggggtc ccaaggaaaa aatggtttga aagaaagaag tactagtata tcaaaagaaa tagttaaaaa tagttttaat aatagtaaat taagacctgg tatgttcata caaaatagta atgtagtatt taatgagcaa tataaaggaa tcaagatttt aggaaagggt tcatttggtg aagttatatt aagtaaagat aaacatacag gtcatgaata tgcaattaaa gtaattagca aaaaacatgt taaaagaaaa acagataaac aaagtctttt aagagaagta gaattattaa aaatgttaga tcatataaat ataatgaaat tatatgaatt ttttgaagat aataattatt attatttagt gtctgatgta tattctggag gtgaattatt tgatgaaatt attagtagga aacgatttta tgaagtagat gcagctagaa taattaaaca agtattaagt ggtattacat atatgcataa aaataatgta gttcatagag atttaaaacc tgaaaatatt cttctcgaaa caaaaaataa agaagatatg attataaaaa ttattgattt tggattatca acccattttg aatatagtaa aaaaatgaaa gataaaatag gcactgccta ttatattgct ccggatgttt tacacggaac ttatgatgaa aaatgtgata tatggtcatg tggtgtcata ttatatattc tcttatcagg atgtccacct tttaatggat ctaatgaata tgacattttg aaaaaagttg aaactggaaa atatactttt gatttacctc aatttaaaaa aataagtgac aaagcgaaag acttgataaa aaaaatgttg atgtacacaa gtgctgtgag aatatcagca agagatgcat tagaacatga atggataaga ttaatgacaa gcaaagataa tgtaaacatt gatattccat ctcttgaatt aagtattaca aatataaaac aatttcaaag tacacaaaag ttagcacaag ccgcactatt atatatggga tcaaaattaa caacaataga tgaaacaaaa gaacttacta aaatttttaa aaaaatggac aaaaatgggg atggtcaatt agacagaaat gaattaataa taggatataa agaattgtta aaattaaaag gagatgatac aactgattta gataatgcag ctattgaagt tgaagtcgat caaatattaa gttctataga tttagatcaa aatggatata tagaatattc agaattttta acagttgcta ttgatagaaa attattatta tcaactgaaa gattagaaaa ggcatttaaa ttatttgata aagatggttc aggaaaaata tctgcaaatg aattagctca attgtttgga ttgggagatg ttagttctga ttgttggaaa actgttttga aagaggttga tcaaaataat gatggggaaa ttgattttaa agaatttaga gacatgttaa taaaactatg taactattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at