PhriA.17805.a

Non-structural protein

CENTER ID: PhriA.17805.a
ORGANISM: Phlebovirus Rift valley fever virus
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIA

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PhriA.17805.a.A11.GE33395 full length 1 265
PhriA.17805.a.A12.GE33396 full length 2 240
PhriA.17805.a.A13.GE33397 full length 2 256
PhriA.17805.a.A14.GE33398 full length 10 265
PhriA.17805.a.A15.GE33399 full length 10 240
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|11588.643.CDS.1
RefSeq: YP_003848706.1
UniProt: A2SZX2
ViPR: link
ViPR: link
ViPR: link
ViPR: link
ViPR: link
ViPR: link
ViPR: link
ViPR: link
ViPR: link
ViPR: link
ViPR: link
ViPR: link
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDYFPVISVD LQSGRRVVSV EYFRGDGPPR IPYSMVGPCC VFLMHHRPSH EVRLRFSDFY NVGEFPYRVG LGDFASNVAP PPAKPFQRLI DLIGHMTLSD FTRFPNLKEA ISWPLGEPSL AFFDLSSTRV HRNDDIRRDQ IATLAMRSCK ITNDLEDSFV GLHRMIATEA ILRGIDLCLL PGFDLMYEVA HVQCVRLLQA AKEDISNAVV PNSALIVLME ESLMLRSSLP SMMGRNNWIP VIPPIPDVEM ESEEESDDDG FVEVD
NT Sequence
atggattact ttcctgtgat atctgttgat ttgcagagtg gtcgtcgtgt tgtgtcagtg gagtacttta gaggagatgg tcctcccagg ataccttatt ctatggttgg gccctgttgt gtctttctca tgcaccatcg tcctagtcac gaggttcgct tgcgattctc tgatttctac aatgtcggag aattcccata ccgagtcgga cttggagact ttgcatcaaa cgttgcacct ccaccagcga agccttttca gagacttatt gatctaatag gccatatgac tcttagtgat ttcacaaggt tccccaatct gaaagaagcc atatcctggc ctcttggaga accctcactg gctttctttg acctaagctc tactagagtg cataggaatg atgacattag aagggatcag attgccactc tagcaatgag gagttgcaag atcaccaatg atctagagga ctcctttgtt ggcttacaca ggatgatagc gactgaggcc atcctcagag ggattgacct gtgcctgttg ccaggctttg atctcatgta tgaggttgct cacgtacagt gcgttcggct tctgcaagca gcaaaagagg acatttctaa tgctgtagtt ccaaactcag ccctcattgt tcttatggag gagagcctga tgctgcgctc atcacttccc agcatgatgg ggagaaacaa ctggattcca gttattcctc caatcccaga tgttgagatg gaatcagagg aggagagtga tgatgatgga tttgttgagg ttgattag
Details for PhriA.17805.a.A11.GE33395
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MDYFPVISVD LQSGRRVVSV EYFRGDGPPR IPYSMVGPCC VFLMHHRPSH EVRLRFSDFY NVGEFPYRVG LGDFASNVAP PPAKPFQRLI DLIGHMTLSD FTRFPNLKEA ISWPLGEPSL AFFDLSSTRV HRNDDIRRDQ IATLAMRSCK ITNDLEDSFV GLHRMIATEA ILRGIDLCLL PGFDLMYEVA HVQCVRLLQA AKEDISNAVV PNSALIVLME ESLMLRSSLP SMMGRNNWIP VIPPIPDVEM ESEEESDDDG FVEVD
Validated NT Sequence
ttcgggcttt gttagcagcc ggancctcga gaagcttggc tgcagaactt gttcgtgctg tttattagtc cacttcaaca aagccgtcgt cgtcgctttc ctcttcggat tccatttcca catccggaat cggcgggatt acaggaatcc agttgttgcg gcccatcatg gacggcagag aggaacgcag catcagggat tcttccatca gcacaatcag tgcggagttt ggaactacgg cgttggagat gtcctccttt gcagcttgga gcagacgaac acactgcacg tgagcaactt cgtacatcag gtcaaagccc ggaagcagac agagatcaat accgcggaga atggcttcgg tggcgatcat gcgatgcagg ccaacgaagc tatcttccag atcgttggta atcttgcaag aacgcatggc gagggttgca atctgatcgc ggcgaatgtc atcgttacga tgaacgcgag tggaagacag gtcgaagaat gccagggacg gttcacccag aggccaggag attgcttctt tcaggttagg gaaacgggtg aaatcagaca gagtcatgtg accaatgaga tcgatcagac gctggaaagg tttagccgga ggcggtgcga cgttggatgc gaaatcgccc aggccaacac ggtatgggaa ttcgcccacg ttatagaagt cggagaagcg gagacgcact tcgtgagacg ggcggtggtg catgaggaac acgcagcacg gaccaaccat agaatacggg atgcgcggag ggccgtcgcc acggaaatat tcaacagata caacacggcg gcctgattgc aggtcaacgg agataactgg gaaatagtcc atcgaaccag accctgggtc tgagcttccn gggtacccat atgatggtga tggngatgag ccatggtata tctcntntaa agttaaacaa aattatttct agagggaaac cgt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDYFPVISV DLQSGRRVVS VEYFRGDGPP RIPYSMVGPC CVFLMHHRPS HEVRLRFSDF YNVGEFPYRV GLGDFASNVA PPPAKPFQRL IDLIGHMTLS DFTRFPNLKE AISWPLGEPS LAFFDLSSTR VHRNDDIRRD QIATLAMRSC KITNDLEDSF VGLHRMIATE AILRGIDLCL LPGFDLMYEV AHVQCVRLLQ AAKEDISNAV VPNSALIVLM EESLMLRSSL PSMMGRNNWI PVIPPIPDVE MESEEESDDD GFVEVD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggactatttc ccagttatct ccgttgacct gcaatcaggc cgccgtgttg tatctgttga atatttccgt ggcgacggcc ctccgcgcat cccgtattct atggttggtc cgtgctgcgt gttcctcatg caccaccgcc cgtctcacga agtgcgtctc cgcttctccg acttctataa cgtgggcgaa ttcccatacc gtgttggcct gggcgatttc gcatccaacg tcgcaccgcc tccggctaaa cctttccagc gtctgatcga tctcattggt cacatgactc tgtctgattt cacccgtttc cctaacctga aagaagcaat ctcctggcct ctgggtgaac cgtccctggc attcttcgac ctgtcttcca ctcgcgttca tcgtaacgat gacattcgcc gcgatcagat tgcaaccctc gccatgcgtt cttgcaagat taccaacgat ctggaagata gcttcgttgg cctgcatcgc atgatcgcca ccgaagccat tctccgcggt attgatctct gtctgcttcc gggctttgac ctgatgtacg aagttgctca cgtgcagtgt gttcgtctgc tccaagctgc aaaggaggac atctccaacg ccgtagttcc aaactccgca ctgattgtgc tgatggaaga atccctgatg ctgcgttcct ctctgccgtc catgatgggc cgcaacaact ggattcctgt aatcccgccg attccggatg tggaaatgga atccgaagag gaaagcgacg acgacggctt tgttgaagtg gactaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for PhriA.17805.a.A12.GE33396
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDYFPVISV DLQSGRRVVS VEYFRGDGPP RIPYSMVGPC CVFLMHHRPS HEVRLRFSDF YNVGEFPYRV GLGDFASNVA PPPAKPFQRL IDLIGHMTLS DFTRFPNLKE AISWPLGEPS LAFFDLSSTR VHRNDDIRRD QIATLAMRSC KITNDLEDSF VGLHRMIATE AILRGIDLCL LPGFDLMYEV AHVQCVRLLQ AAKEDISNAV VPNSALIVLM EESLMLRSSL PSMMGRNNWI P
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggactatttc ccagttatct ccgttgacct gcaatcaggc cgccgtgttg tatctgttga atatttccgt ggcgacggcc ctccgcgcat cccgtattct atggttggtc cgtgctgcgt gttcctcatg caccaccgcc cgtctcacga agtgcgtctc cgcttctccg acttctataa cgtgggcgaa ttcccatacc gtgttggcct gggcgatttc gcatccaacg tcgcaccgcc tccggctaaa cctttccagc gtctgatcga tctcattggt cacatgactc tgtctgattt cacccgtttc cctaacctga aagaagcaat ctcctggcct ctgggtgaac cgtccctggc attcttcgac ctgtcttcca ctcgcgttca tcgtaacgat gacattcgcc gcgatcagat tgcaaccctc gccatgcgtt cttgcaagat taccaacgat ctggaagata gcttcgttgg cctgcatcgc atgatcgcca ccgaagccat tctccgcggt attgatctct gtctgcttcc gggctttgac ctgatgtacg aagttgctca cgtgcagtgt gttcgtctgc tccaagctgc aaaggaggac atctccaacg ccgtagttcc aaactccgca ctgattgtgc tgatggaaga atccctgatg ctgcgttcct ctctgccgtc catgatgggc cgcaacaact ggattcctta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for PhriA.17805.a.A13.GE33397
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 92
Validated AA Sequence
MDYFPXISVD LQSGRRVVSV EYFRGDGPPR IPYSMVGPCC VFLMHHRPSH EVRLRFSDFY NVGEFPYRVG LGDFASNVAP PPAKPFQRLI DLIGHMTLSD FTRFPNLKEA ISWPLGEPSL AFFDLSSTRV HRNDDIRRDQ IATLAMRSCK ITNDLEDSFV GLHRMIATEA ILRGIDLCLL PGFDLMYEVA HVQCVRLLQA AKEDISNAVV PNSALIVLME ESLMLRSSLP SMMGRNNWIP VIPPIPDVEM ESEEES
Validated NT Sequence
cnttcgggct ttgttagcag ccggatcctc gagaagcttg gctgcagaac ttgttcgtgc tgtttattag ctttcctctt cggattccat ttccacatcc ggaatcggcg ggattacagg aatccagttg ttgcggccca tcatggacgg cagagaggaa cgcagcatca gggattcttc catcagcaca atcagtgcgg agtttggaac tacggcgttg gagatgtcct cctttgcagc ttggagcaga cgaacacact gcacgtgagc aacttcgtac atcaggtcaa agcccggaag cagacagaga tcaataccgc ggagaatggc ttcggtggcg atcatgcgat gcaggccaac gaagctatct tccagatcgt tggtaatctt gcaagaacgc atggcgaggg ttgcaatctg atcgcggcga atgtcatcgt tacgatgaac gcgagtggaa gacaggtcga agaatgccag ggacggttca cccagaggcc aggagattgc ttctttcagg ttagggaaac gggtgaaatc agacagagtc atgtgaccaa tgagatcgat cagacgctgg aaaggtttag ccggaggcgg tgcgacgttg gatgcgaaat cgcccaggcc aacacggtat gggaattcgc ccacgttata gaagtcggag aagcggagac gcacttcgtg agacgggcgg tggtgcatga ggaacacgca gcacggacca accatagaat acgggatgcg cggagggccg tcgccacgga aatattcaac agatacaaca cggcggcctg attgcaggtc aacggagata nctgggaaat agtccatcga accnnnccct gggtctgagc ttccngggta nccatatgan ggtgatggtg atgagccatg gtatntctcc tnnttaaagt taaacaaaat tatttctaga gggnaancgt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDYFPVISV DLQSGRRVVS VEYFRGDGPP RIPYSMVGPC CVFLMHHRPS HEVRLRFSDF YNVGEFPYRV GLGDFASNVA PPPAKPFQRL IDLIGHMTLS DFTRFPNLKE AISWPLGEPS LAFFDLSSTR VHRNDDIRRD QIATLAMRSC KITNDLEDSF VGLHRMIATE AILRGIDLCL LPGFDLMYEV AHVQCVRLLQ AAKEDISNAV VPNSALIVLM EESLMLRSSL PSMMGRNNWI PVIPPIPDVE MESEEES
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggactatttc ccagttatct ccgttgacct gcaatcaggc cgccgtgttg tatctgttga atatttccgt ggcgacggcc ctccgcgcat cccgtattct atggttggtc cgtgctgcgt gttcctcatg caccaccgcc cgtctcacga agtgcgtctc cgcttctccg acttctataa cgtgggcgaa ttcccatacc gtgttggcct gggcgatttc gcatccaacg tcgcaccgcc tccggctaaa cctttccagc gtctgatcga tctcattggt cacatgactc tgtctgattt cacccgtttc cctaacctga aagaagcaat ctcctggcct ctgggtgaac cgtccctggc attcttcgac ctgtcttcca ctcgcgttca tcgtaacgat gacattcgcc gcgatcagat tgcaaccctc gccatgcgtt cttgcaagat taccaacgat ctggaagata gcttcgttgg cctgcatcgc atgatcgcca ccgaagccat tctccgcggt attgatctct gtctgcttcc gggctttgac ctgatgtacg aagttgctca cgtgcagtgt gttcgtctgc tccaagctgc aaaggaggac atctccaacg ccgtagttcc aaactccgca ctgattgtgc tgatggaaga atccctgatg ctgcgttcct ctctgccgtc catgatgggc cgcaacaact ggattcctgt aatcccgccg attccggatg tggaaatgga atccgaagag gaaagctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for PhriA.17805.a.A14.GE33398
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGXG SMDXQSGRRV VSVEYFRGDG XPRIPYSMVG PCCVFLMHHR PSHEVRLRFS DFYNVGEFPY RVGLGDFASN VAPPPAKPFQ RLIDLIGHMT LSDFTRFPNL KEAISWPLGE PSLAFFDLSS TRVHRNDDIR RDQIATLAMR SCKITNDLED SFVGLHRMIA TEAILRGIDL CLLPGFDLMY EVAHVQCVRL LQAAKEDISN AVVPNSALIV LMEESLMLRS SLPSMMGRNN WIPVIPPIPD VEMESEEESD DDGFVEVD
Validated NT Sequence
cnttcgggct ttgttagcag ccggancctc gagaagcttg gctgcagaac ttgttcgtgc tgtttattag tccacttcaa caaagccgtc gtcgtcgctt tcctcttcgg attccatttc cacatccgga atcggcggga ttacaggaat ccagttgttg cggcccatca tggacggcag agaggaacgc agcatcaggg attcttccat cagcacaatc agtgcggagt ttggaactac ggcgttggag atgtcctcct ttgcagcttg gagcagacga acacactgca cgtgagcaac ttcgtacatc aggtcaaagc ccggaagcag acagagatca ataccgcgga gaatggcttc ggtggcgatc atgcgatgca ggccaacgaa gctatcttcc agatcgttgg taatcttgca agaacgcatg gcgagggttg caatctgatc gcggcgaatg tcatcgttac gatgaacgcg agtggaagac aggtcgaaga atgccaggga cggttcaccc agaggccagg agattgcttc tttcaggtta gggaaacggg tgaaatcaga cagagtcatg tgaccaatga gatcgatcag acgctggaaa ggtttagccg gaggcggtgc gacgttggat gcgaaatcgc ccaggccaac acggtatggg aattcgccca cgttatagaa gtcggagaag cggagacgca cttcgtgaga cgggcggtgg tgcatgagga acacgcagca cggaccaacc atagaatacg ggatgcgcgg anggccgtcg ccacggaaat attcaacaga tacaacacgg cggcctgatt gcangtccat cgaaccnnna ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc catggtatat ctnnnnntta aagttaaacn aaattanttt ctagagggaa accgttgng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLQSGRRV VSVEYFRGDG PPRIPYSMVG PCCVFLMHHR PSHEVRLRFS DFYNVGEFPY RVGLGDFASN VAPPPAKPFQ RLIDLIGHMT LSDFTRFPNL KEAISWPLGE PSLAFFDLSS TRVHRNDDIR RDQIATLAMR SCKITNDLED SFVGLHRMIA TEAILRGIDL CLLPGFDLMY EVAHVQCVRL LQAAKEDISN AVVPNSALIV LMEESLMLRS SLPSMMGRNN WIPVIPPIPD VEMESEEESD DDGFVEVD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacctgcaa tcaggccgcc gtgttgtatc tgttgaatat ttccgtggcg acggccctcc gcgcatcccg tattctatgg ttggtccgtg ctgcgtgttc ctcatgcacc accgcccgtc tcacgaagtg cgtctccgct tctccgactt ctataacgtg ggcgaattcc cataccgtgt tggcctgggc gatttcgcat ccaacgtcgc accgcctccg gctaaacctt tccagcgtct gatcgatctc attggtcaca tgactctgtc tgatttcacc cgtttcccta acctgaaaga agcaatctcc tggcctctgg gtgaaccgtc cctggcattc ttcgacctgt cttccactcg cgttcatcgt aacgatgaca ttcgccgcga tcagattgca accctcgcca tgcgttcttg caagattacc aacgatctgg aagatagctt cgttggcctg catcgcatga tcgccaccga agccattctc cgcggtattg atctctgtct gcttccgggc tttgacctga tgtacgaagt tgctcacgtg cagtgtgttc gtctgctcca agctgcaaag gaggacatct ccaacgccgt agttccaaac tccgcactga ttgtgctgat ggaagaatcc ctgatgctgc gttcctctct gccgtccatg atgggccgca acaactggat tcctgtaatc ccgccgattc cggatgtgga aatggaatcc gaagaggaaa gcgacgacga cggctttgtt gaagtggact aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for PhriA.17805.a.A15.GE33399
HARVESTED ON: 9/12/2011
SEQUENCED ON: 10/23/2013
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDLQSGRRV VSVEYFRGDG PPRIPYSMVG PCCVFLMHHR PSHEVRLRFS DFYNVGEFPY RVGLGDFASN VAPPPAKPFQ RLIDLIGHMT LSDFTRFPNL KEAISWPLGE PSLAFFDLSS TRVHRNDDIR RDQIATLAMR SCKITNDLED SFVGLHRMIA TEAILRGIDL CLLPGFDLMY EVAHVQCVRL LQAAKEDISX AVXPNSALIV LMEESLMLRS SLPSMMGRNN WIP
Validated NT Sequence
attttnttta ctttaagang gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacc tgcaatcagg ccgccgtgtt gtatctgttg aatatttccg tggcgacggc cctccgcgca tcccgtattc tatggttggt ccgtgctgcg tgttcctcat gcaccaccgc ccgtctcacg aagtgcgtct ccgcttctcc gacttctata acgtgggcga attcccatac cgtgttggcc tgggcgattt cgcatccaac gtcgcaccgc ctccggctaa acctttccag cgtctgatcg atctcattgg tcacatgact ctgtctgatt tcacccgttt ccctaacctg aaagaagcaa tctcctggcc tctgggtgaa ccgtccctgg cattcttcga cctgtcttcc actcgcgttc atcgtaacga tgacattcgc cgcgatcaga ttgcaaccct cgccatgcgt tcttgcaaga ttaccaacga tctggaagat agcttcgttg gcctgcatcg catgatcgcc accgaagcca ttctccgcgg tattgatctc tgtctgcttc cgggctttga cctgatgtac gaagttgctc acgtgcagtg tgttcgtctg ctccaagctg caaaggagga catctccnac gccgtanttc caaactccgc actgattgtg ctgatggaag aatccctgat gctgcgttcc tctctgccgt ccatgatggg ccgcaacaac tggattcctt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccngct gcnnnnnnnc cnnnnnnnnn nnangnnaaa nnnnnnannn gnnnnnnnnn nnngntcnnc nngaangtgg annnnntcan caannnnngn nnnnnnnntn nnannannnn tgggnnnnnn gnacnnnnnn annnnnnntn nncaanttnn nnncgncnnn ngnantncnn nnnnnntnnn nnnnnnnann nnnnagaaan nnnnnnnncg gnnnnnntnn nnnnnnnnnn ngnnnaannn nnnnnnnnnn nnnnanncng ngnacnnang nnnncnnnnn nnnnnnnntn nnnnngnnnn nnnnnnnnnn nnnnnnncnn nngannnnnn nncnnannnn nnnnnaaaan nnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLQSGRRV VSVEYFRGDG PPRIPYSMVG PCCVFLMHHR PSHEVRLRFS DFYNVGEFPY RVGLGDFASN VAPPPAKPFQ RLIDLIGHMT LSDFTRFPNL KEAISWPLGE PSLAFFDLSS TRVHRNDDIR RDQIATLAMR SCKITNDLED SFVGLHRMIA TEAILRGIDL CLLPGFDLMY EVAHVQCVRL LQAAKEDISN AVVPNSALIV LMEESLMLRS SLPSMMGRNN WIP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacctgcaa tcaggccgcc gtgttgtatc tgttgaatat ttccgtggcg acggccctcc gcgcatcccg tattctatgg ttggtccgtg ctgcgtgttc ctcatgcacc accgcccgtc tcacgaagtg cgtctccgct tctccgactt ctataacgtg ggcgaattcc cataccgtgt tggcctgggc gatttcgcat ccaacgtcgc accgcctccg gctaaacctt tccagcgtct gatcgatctc attggtcaca tgactctgtc tgatttcacc cgtttcccta acctgaaaga agcaatctcc tggcctctgg gtgaaccgtc cctggcattc ttcgacctgt cttccactcg cgttcatcgt aacgatgaca ttcgccgcga tcagattgca accctcgcca tgcgttcttg caagattacc aacgatctgg aagatagctt cgttggcctg catcgcatga tcgccaccga agccattctc cgcggtattg atctctgtct gcttccgggc tttgacctga tgtacgaagt tgctcacgtg cagtgtgttc gtctgctcca agctgcaaag gaggacatct ccaacgccgt agttccaaac tccgcactga ttgtgctgat ggaagaatcc ctgatgctgc gttcctctct gccgtccatg atgggccgca acaactggat tccttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa