SSGCID: Target Page

OrpoA.17277.a

N protein

CENTER ID:	OrpoA.17277.a
ORGANISM:	Orthobunyavirus Pongola virus SAAr1
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
OrpoA.17277.a.A11.GE33413	Full length( OrpoA.17277.a )		1	235

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|537994.11.CDS.1
UniProt:	B3U4Y0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGDLVFYDV DSVNSNAFDP DAGYVAFSNN HPGALNITTA RTFFLNAAKA KGVLRNKPDK KVKPKFGDWE VEIVNNHFPG NRNNTIGEDD LTLHRISGYL ARWVLEEHSK DDESEKELIE STIVNPIAES NGIKWSDGAA IYLSFFPGTE MFLEPFKFFP LAIGIYRVKH KMMDAQFLKK ALRQRYGKLT AEKWMATKVK SIGEAVRAVE KLKWGKGGIS DAARTFLKKF DIALI

NT Sequence

ATGTCGGGAG ATCTAGTTTT TTATGATGTC GACTCCGTCA ATTCCAATGC GTTTGATCCT GATGCAGGAT ATGTGGCATT TTCAAACAAC CATCCAGGGG CGCTTAATAT CACTACAGCT AGGACATTTT TCCTCAATGC CGCAAAGGCC AAGGGTGTCC TCCGTAATAA ACCTGACAAG AAGGTTAAAC CTAAATTTGG AGACTGGGAG GTGGAGATTG TCAATAATCA TTTTCCTGGA AACAGGAACA ATACAATTGG TGAAGACGAT CTTACCCTCC ACAGGATTTC AGGATATTTA GCTCGTTGGG TTCTTGAAGA ACATTCTAAA GATGATGAAA GTGAGAAAGA ATTAATTGAG AGTACAATTG TGAACCCCAT TGCTGAGTCA AATGGGATAA AGTGGAGCGA TGGTGCAGCA ATTTACTTAT CATTCTTCCC AGGAACTGAA ATGTTTCTTG AACCGTTTAA GTTCTTTCCA CTTGCAATTG GAATCTACCG CGTTAAGCAT AAAATGATGG ATGCCCAATT CCTTAAGAAG GCCTTGCGTC AAAGATATGG GAAACTTACA GCAGAAAAGT GGATGGCAAC AAAGGTCAAA TCAATTGGAG AAGCTGTTAG AGCTGTAGAG AAACTGAAGT GGGGAAAAGG AGGAATTAGT GATGCAGCCA GGACTTTCCT TAAGAAATTT GATATTGCAC TAATC

Details for OrpoA.17277.a.A11.GE33413

HARVESTED ON:	9/12/2011
SEQUENCED ON:	9/20/2011
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSGDLVFYD VDSVNSNAFD PDAGYVAFSN NHPGALNITT ARTFFLNAAK AKGVLRNKPD KKVKPKFGDW EVEIVNNHFP GNRNNTIGED DLTLHRISGY LARWVLEEHS KDDESEKELI ESTIVNPIAE SNGIKWSDGA AIYLSFFPGT EMFLEPFKFF PLAIGIYRVK HKMMDAQFLK KALRQRYGKL TAEKWMATKV KSIGEAVRAV EKLKWGKGGI SDAARTFLKK FDIALI

Validated NT Sequence

ttattagatc agagcgatat caaatttctt caggaaggta cgagcggcgt cgctaatacc acctttaccc cacttgagct tctccacagc acgaacagct tcaccaatgc tcttaacttt agtggccatc catttttcgg cggtcagttt gccgtagcgc tggcggagag ctttcttcag aaactgagcg tccatcatct tgtgtttcac acggtagatg ccgatggcca atgggaagaa tttgaacggt tcgagaaaca tttcggtacc cggaaagaag gacaggtaga tagcagcacc atcagaccac ttgatgccgt tggattcagc gattgggttc acgatggtcg attcgatcag ttctttctca gattcgtcgt ctttggagtg ttcttccagg acccaacgag cgagataacc agagatgcgg tgtaaggtca ggtcgtcttc accaatggta ttattacgat tgcctgggaa gtggttgttc acaatttcaa cctcccaatc gccgaacttc ggtttaactt tcttatcagg tttattgcgc agaacaccct ttgctttagc tgcgttgagg aagaaagtac gggcagtggt gatgttcagt gcacccggat ggttattaga gaatgcaacg tagcctgcgt ctgggtcgaa tgcgttgctg ttcacagagt caacgtcgta aaacaccaga tcgccggaca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccggcgat ctggtgtttt acgacgttga ctctgtgaac agcaacgcat tcgacccaga cgcaggctac gttgcattct ctaataacca tccgggtgca ctgaacatca ccactgcccg tactttcttc ctcaacgcag ctaaagcaaa gggtgttctg cgcaataaac ctgataagaa agttaaaccg aagttcggcg attgggaggt tgaaattgtg aacaaccact tcccaggcaa tcgtaataat accattggtg aagacgacct gaccttacac cgcatctctg gttatctcgc tcgttgggtc ctggaagaac actccaaaga cgacgaatct gagaaagaac tgatcgaatc gaccatcgtg aacccaatcg ctgaatccaa cggcatcaag tggtctgatg gtgctgctat ctacctgtcc ttctttccgg gtaccgaaat gtttctcgaa ccgttcaaat tcttcccatt ggccatcggc atctaccgtg tgaaacacaa gatgatggac gctcagtttc tgaagaaagc tctccgccag cgctacggca aactgaccgc cgaaaaatgg atggccacta aagttaagag cattggtgaa gctgttcgtg ctgtggagaa gctcaagtgg ggtaaaggtg gtattagcga cgccgctcgt accttcctga agaaatttga tatcgctctg atctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa