OrbuA.17277.b

Nucleocapsid protein

CENTER ID: OrbuA.17277.b
ORGANISM: Orthobunyavirus Bunyamwera virus
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
OrbuA.17277.b.A11.GE33431 Full length( OrbuA.17277.b ) 1 233
OrbuA.17277.b.A13.GE33433 Full length( OrbuA.17277.b ) 2 230
OrbuA.17277.b.A14.GE33434 Full length( OrbuA.17277.b ) 10 233
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
OrbuA.17277.b.A11.PW34041 Full length( OrbuA.17277.b ) 1 233
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|35304.86.CDS.1
UniProt: Q6KC67
ViPR: link
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEIAFDDIG QIAASTFNPD NQYIGFKKVF TIGLTYDQIR IFFLNGKKAK ETLSKRSEET VTLNFGGWRI PIVNTHFPGN RNIDLADDAL TLHRVSGYLA RYLLEKVLSA QEPEKVIIKT RIINPIAASN GITWDDGYEV YLSFFPGSEC FLEAFKFYPL AIGIYKVQKG MMDVKFLEKT MRQKYAGLDA TVWTQQKYTD VINALLVVNG LGWKKSNVSA AAKDFLSKFG IQI
NT Sequence
ATGTCTGAAA TTGCATTTGA TGATATTGGC CAAATTGCAG CAAGCACTTT CAATCCGGAC AATCAGTACA TAGGCTTTAA AAAGGTCTTT ACTATTGGGC TTACTTATGA TCAAATCAGA ATTTTCTTCC TGAATGGGAA GAAGGCCAAG GAGACATTAT CTAAAAGAAG TGAAGAAACT GTCACTCTGA ATTTCGGTGG CTGGAGGATT CCTATCGTTA ATACTCATTT TCCGGGGAAT CGAAATATTG ACCTTGCAGA TGATGCACTC ACTCTGCACA GAGTAAGCGG CTACTTGGCC AGATACTTAC TTGAAAAGGT CTTGAGCGCC CAGGAACCTG AGAAAGTGAT TATAAAGACA AGAATAATCA ATCCAATTGC TGCTTCTAAT GGGATCACAT GGGATGATGG GTATGAAGTC TATCTCTCTT TCTTTCCTGG CTCTGAATGT TTTCTGGAAG CTTTTAAATT CTACCCTCTA GCCATCGGAA TCTACAAAGT GCAGAAGGGA ATGATGGATG TTAAGTTCCT AGAAAAAACA ATGAGGCAAA AATATGCTGG ACTTGATGCA ACTGTTTGGA CTCAGCAGAA GTACACAGAC GTCATCAATG CCCTCCTTGT TGTAAATGGC CTTGGCTGGA AGAAGTCAAA CGTGAGTGCT GCTGCTAAAG ACTTCCTGTC CAAATTCGGA ATTCAGATT
Details for OrbuA.17277.b.A11.GE33431
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIAFDDI GQIAASTFNP DNQYIGFKKV FTIGLTYDQI RIFFLNGKKA KETLSKRSEE TVTLNFGGWR IPIVNTHFPG NRNIDLADDA LTLHRVSGYL ARYLLEKVLS AQEPEKVIIK TRIINPIAAS NGITWDDGYE VYLSFFPGSE CFLEAFKFYP LAIGIYKVQK GMMDVKFLEK TMRQKYAGLD ATVWTQQKYT DVINALLVVN GLGWKKSNVS AAAKDFLSKF G
Validated NT Sequence
ataccgaatt tagacaggaa gtccttagca gctgcggaca cgttagactt cttccagccc agaccattca caaccagcag tgcgttaata acgtcggtgt acttctgctg ggtccaaacg gttgcgtcca ggccggcgta tttctggcgc atagtttttt cgaggaattt cacgtccatc atacctttct gcactttata aataccaata gccagtgggt agaacttgaa tgcttcgagg aaacattcgc tgcccggaaa gaaagagagg taaacttcgt aaccgtcatc ccaggtgatg ccgttggagg cagcgatagg gttaataatg cgggtcttga tgattacttt ctccggctcc tgagcagaga gaactttctc cagcagataa cgggcgaggt agccggaaac acggtgcaga gtcagggcat cgtctgcgag atcaatgtta cggttgcctg gaaagtgggt gttaacgata gggatacgcc agccgccaaa gttcagggta acggtctctt cggagcgttt agacagagtt tccttagcct tcttgccgtt aagaaagaag atgcggatct ggtcgtaggt caggccgatg gtaaacacct tcttgaagcc aatgtactgg ttatccgggt tgaaagtgga tgctgcgatc tggccgatgt cgtcgaatgc gatttcggac atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIAFDDI GQIAASTFNP DNQYIGFKKV FTIGLTYDQI RIFFLNGKKA KETLSKRSEE TVTLNFGGWR IPIVNTHFPG NRNIDLADDA LTLHRVSGYL ARYLLEKVLS AQEPEKVIIK TRIINPIAAS NGITWDDGYE VYLSFFPGSE CFLEAFKFYP LAIGIYKVQK GMMDVKFLEK TMRQKYAGLD ATVWTQQKYT DVINALLVVN GLGWKKSNVS AAAKDFLSKF GIQI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgaaatc gcattcgacg acatcggcca gatcgcagca tccactttca acccggataa ccagtacatt ggcttcaaga aggtgtttac catcggcctg acctacgacc agatccgcat cttctttctt aacggcaaga aggctaagga aactctgtct aaacgctccg aagagaccgt taccctgaac tttggcggct ggcgtatccc tatcgttaac acccactttc caggcaaccg taacattgat ctcgcagacg atgccctgac tctgcaccgt gtttccggct acctcgcccg ttatctgctg gagaaagttc tctctgctca ggagccggag aaagtaatca tcaagacccg cattattaac cctatcgctg cctccaacgg catcacctgg gatgacggtt acgaagttta cctctctttc tttccgggca gcgaatgttt cctcgaagca ttcaagttct acccactggc tattggtatt tataaagtgc agaaaggtat gatggacgtg aaattcctcg aaaaaactat gcgccagaaa tacgccggcc tggacgcaac cgtttggacc cagcagaagt acaccgacgt tattaacgca ctgctggttg tgaatggtct gggctggaag aagtctaacg tgtccgcagc tgctaaggac ttcctgtcta aattcggtat ccagatttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for OrbuA.17277.b.A13.GE33433
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 96
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIAFDDI GQIAASTFNP DNQYIGFKKV FTIGLTYDQI RIFFLNGKKA KETLSKRSEE TVTLNFGGWR IPIVNTHFPG NRNIDLADDA LTLHRVSGYL ARYLLEKVLS AQEPEKVIIK TRIINPIAAS NGITWDDGYE VYLSFFPGSE CFLEAFKFYP LAIGIYKVQK GMMDVKFLEK TMRQKYAGLD ATVWTQQKYT DVINALLVVN GLGWKKSNVS AAAKDFLSKF G
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtccg aaatcgcatt cgacgacatc ggccagatcg cagcatccac tttcaacccg gataaccagt acattggctt caagaaggtg tttaccatcg gcctgaccta cgaccagatc cgcatcttct ttcttaacgg caagaaggct aaggaaactc tgtctaaacg ctccgaagag accgttaccc tgaactttgg cggctggcgt atccctatcg ttaacaccca ctttccaggc aaccgtaaca ttgatctcgc agacgatgcc ctgactctgc accgtgtttc cggctacctc gcccgttatc tgctggagaa agttctctct gctcaggagc cggagaaagt aatcatcaag acccgcatta ttaaccctat cgctgcctcc aacggcatca cctgggatga cggttacgaa gtttacctct ctttctttcc gggcagcgaa tgtttcctcg aagcattcaa gttctaccca ctggctattg gtatttataa agtgcagaaa ggtatgatgg acgtgaaatt cctcgaaaaa actatgcgcc agaaatacgc cggcctggac gcaaccgttt ggacccagca gaagtacacc gacgttatta acgcactgct ggttgtgaat ggtctgggct ggaagaagtc taacgtgtcc gcagctgcta aggacttcct gtctaaattc ggttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIAFDDI GQIAASTFNP DNQYIGFKKV FTIGLTYDQI RIFFLNGKKA KETLSKRSEE TVTLNFGGWR IPIVNTHFPG NRNIDLADDA LTLHRVSGYL ARYLLEKVLS AQEPEKVIIK TRIINPIAAS NGITWDDGYE VYLSFFPGSE CFLEAFKFYP LAIGIYKVQK GMMDVKFLEK TMRQKYAGLD ATVWTQQKYT DVINALLVVN GLGWKKSNVS AAAKDFLSKF G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgaaatc gcattcgacg acatcggcca gatcgcagca tccactttca acccggataa ccagtacatt ggcttcaaga aggtgtttac catcggcctg acctacgacc agatccgcat cttctttctt aacggcaaga aggctaagga aactctgtct aaacgctccg aagagaccgt taccctgaac tttggcggct ggcgtatccc tatcgttaac acccactttc caggcaaccg taacattgat ctcgcagacg atgccctgac tctgcaccgt gtttccggct acctcgcccg ttatctgctg gagaaagttc tctctgctca ggagccggag aaagtaatca tcaagacccg cattattaac cctatcgctg cctccaacgg catcacctgg gatgacggtt acgaagttta cctctctttc tttccgggca gcgaatgttt cctcgaagca ttcaagttct acccactggc tattggtatt tataaagtgc agaaaggtat gatggacgtg aaattcctcg aaaaaactat gcgccagaaa tacgccggcc tggacgcaac cgtttggacc cagcagaagt acaccgacgt tattaacgca ctgctggttg tgaatggtct gggctggaag aagtctaacg tgtccgcagc tgctaaggac ttcctgtcta aattcggtta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for OrbuA.17277.b.A14.GE33434
HARVESTED ON: 9/12/2011
SEQUENCED ON: 9/20/2011
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGQIAASTF NPDNQYIGFK KVFTIGLTYD QIRIFFLNGK KAKETLSKRS EETVTLNFGG WRIPIVNTHF PGNRNIDLAD DALTLHRVSG YLARYLLEKV LSAQEPEKVI IKTRIINPIA ASNGITWDDG YEVYLSFFPG SECFLEAFKF YPLAIGIYKV QKGMMDVKFL EKTMRQKYAG LDATVWTQQK YTDVINALLV VNGLGWKKSN VSAAAKDFLS KFG
Validated NT Sequence
ataccgaatt tagacaggaa gtccttagca gctgcggaca cgttagactt cttccagccc agaccattca caaccagcag tgcgttaata acgtcggtgt acttctgctg ggtccaaacg gttgcgtcca ggccggcgta tttctggcgc atagtttttt cgaggaattt cacgtccatc atacctttct gcactttata aataccaata gccagtgggt agaacttgaa tgcttcgagg aaacattcgc tgcccggaaa gaaagagagg taaacttcgt aaccgtcatc ccaggtgatg ccgttggagg cagcgatagg gttaataatg cgggtcttga tgattacttt ctccggctcc tgagcagaga gaactttctc cagcagataa cgggcgaggt agccggaaac acggtgcaga gtcagggcat cgtctgcgag atcaatgtta cggttgcctg gaaagtgggt gttaacgata gggatacgcc agccgccaaa gttcagggta acggtctctt cggagcgttt agacagagtt tccttagcct tcttgccgtt aagaaagaag atgcggatct ggtcgtaggt caggccgatg gtaaacacct tcttgaagcc aatgtactgg ttatccgggt tgaaagtgga tgctgcgatc tggcccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGQIAASTF NPDNQYIGFK KVFTIGLTYD QIRIFFLNGK KAKETLSKRS EETVTLNFGG WRIPIVNTHF PGNRNIDLAD DALTLHRVSG YLARYLLEKV LSAQEPEKVI IKTRIINPIA ASNGITWDDG YEVYLSFFPG SECFLEAFKF YPLAIGIYKV QKGMMDVKFL EKTMRQKYAG LDATVWTQQK YTDVINALLV VNGLGWKKSN VSAAAKDFLS KFGIQI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggccagatc gcagcatcca ctttcaaccc ggataaccag tacattggct tcaagaaggt gtttaccatc ggcctgacct acgaccagat ccgcatcttc tttcttaacg gcaagaaggc taaggaaact ctgtctaaac gctccgaaga gaccgttacc ctgaactttg gcggctggcg tatccctatc gttaacaccc actttccagg caaccgtaac attgatctcg cagacgatgc cctgactctg caccgtgttt ccggctacct cgcccgttat ctgctggaga aagttctctc tgctcaggag ccggagaaag taatcatcaa gacccgcatt attaacccta tcgctgcctc caacggcatc acctgggatg acggttacga agtttacctc tctttctttc cgggcagcga atgtttcctc gaagcattca agttctaccc actggctatt ggtatttata aagtgcagaa aggtatgatg gacgtgaaat tcctcgaaaa aactatgcgc cagaaatacg ccggcctgga cgcaaccgtt tggacccagc agaagtacac cgacgttatt aacgcactgc tggttgtgaa tggtctgggc tggaagaagt ctaacgtgtc cgcagctgct aaggacttcc tgtctaaatt cggtatccag atttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for OrbuA.17277.b.A11.PW34041
PURIFICATION DATe: 12/9/2011
CONCENTRATION: 30.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIAFDDI GQIAASTFNP DNQYIGFKKV FTIGLTYDQI RIFFLNGKKA KETLSKRSEE TVTLNFGGWR IPIVNTHFPG NRNIDLADDA LTLHRVSGYL ARYLLEKVLS AQEPEKVIIK TRIINPIAAS NGITWDDGYE VYLSFFPGSE CFLEAFKFYP LAIGIYKVQK GMMDVKFLEK TMRQKYAGLD ATVWTQQKYT DVINALLVVN GLGWKKSNVS AAAKDFLSKF G
Validated NT Sequence
ataccgaatt tagacaggaa gtccttagca gctgcggaca cgttagactt cttccagccc agaccattca caaccagcag tgcgttaata acgtcggtgt acttctgctg ggtccaaacg gttgcgtcca ggccggcgta tttctggcgc atagtttttt cgaggaattt cacgtccatc atacctttct gcactttata aataccaata gccagtgggt agaacttgaa tgcttcgagg aaacattcgc tgcccggaaa gaaagagagg taaacttcgt aaccgtcatc ccaggtgatg ccgttggagg cagcgatagg gttaataatg cgggtcttga tgattacttt ctccggctcc tgagcagaga gaactttctc cagcagataa cgggcgaggt agccggaaac acggtgcaga gtcagggcat cgtctgcgag atcaatgtta cggttgcctg gaaagtgggt gttaacgata gggatacgcc agccgccaaa gttcagggta acggtctctt cggagcgttt agacagagtt tccttagcct tcttgccgtt aagaaagaag atgcggatct ggtcgtaggt caggccgatg gtaaacacct tcttgaagcc aatgtactgg ttatccgggt tgaaagtgga tgctgcgatc tggccgatgt cgtcgaatgc gatttcggac atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIAFDDI GQIAASTFNP DNQYIGFKKV FTIGLTYDQI RIFFLNGKKA KETLSKRSEE TVTLNFGGWR IPIVNTHFPG NRNIDLADDA LTLHRVSGYL ARYLLEKVLS AQEPEKVIIK TRIINPIAAS NGITWDDGYE VYLSFFPGSE CFLEAFKFYP LAIGIYKVQK GMMDVKFLEK TMRQKYAGLD ATVWTQQKYT DVINALLVVN GLGWKKSNVS AAAKDFLSKF GIQI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCGAAATC GCATTCGACG ACATCGGCCA GATCGCAGCA TCCACTTTCA ACCCGGATAA CCAGTACATT GGCTTCAAGA AGGTGTTTAC CATCGGCCTG ACCTACGACC AGATCCGCAT CTTCTTTCTT AACGGCAAGA AGGCTAAGGA AACTCTGTCT AAACGCTCCG AAGAGACCGT TACCCTGAAC TTTGGCGGCT GGCGTATCCC TATCGTTAAC ACCCACTTTC CAGGCAACCG TAACATTGAT CTCGCAGACG ATGCCCTGAC TCTGCACCGT GTTTCCGGCT ACCTCGCCCG TTATCTGCTG GAGAAAGTTC TCTCTGCTCA GGAGCCGGAG AAAGTAATCA TCAAGACCCG CATTATTAAC CCTATCGCTG CCTCCAACGG CATCACCTGG GATGACGGTT ACGAAGTTTA CCTCTCTTTC TTTCCGGGCA GCGAATGTTT CCTCGAAGCA TTCAAGTTCT ACCCACTGGC TATTGGTATT TATAAAGTGC AGAAAGGTAT GATGGACGTG AAATTCCTCG AAAAAACTAT GCGCCAGAAA TACGCCGGCC TGGACGCAAC CGTTTGGACC CAGCAGAAGT ACACCGACGT TATTAACGCA CTGCTGGTTG TGAATGGTCT GGGCTGGAAG AAGTCTAACG TGTCCGCAGC TGCTAAGGAC TTCCTGTCTA AATTCGGTAT CCAGATTTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA