OnvoA.17194.a
prolyl-trna synthetase (ProRS)-like protein; uncharacterized protein
| CENTER ID: | OnvoA.17194.a |
| ORGANISM: | Onchocerca volvulus |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | NA |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| OnvoA.17194.a.B1.GE39189 | true full-length | 1 | 550 | ||
| OnvoA.17194.a.B2.GE40762 | potential soluble domain | 58 | 536 | ||
| OnvoA.17194.a.B5.GE40763 | XtalPred structured domain #1 | 54 | 550 | ||
| OnvoA.17194.a.MB1.GE39288 | true full-length | 1 | 550 | ||
| OnvoA.17194.a.MB2.GE39289 | potential soluble domain | 58 | 536 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| OnvoA.17194.a.MB1.PS02533 | true full-length | 1 | 550 | ||
| OnvoA.17194.a.B5.PW38185 | XtalPred structured domain #1 | 54 | 550 |
Structures
6MN8
DEPOSITED: 10/1/2018
DETERMINATION: XRay
CLONE: OnvoA.17194.a.B5.GE40763
PROTEIN: OnvoA.17194.a.B5.PW38185
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| UniProt: | A0A044RF64 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALVNNGDDT LGMKITSQKN AMRKLENNLS KSHISNSTPM KGRGDTKKQT KLGIEISKDE NYSEWYVQVI TKAEMIEYYD ISGCYVLRPW SYAIWEFIQE WFDEEIKKLG VKNCYFPLFV SQSALEKEKT HISDFAPEVA WITRAGQSDL AEAIAIRPTS ETVMYPSYAK WVQSHRDLPI KLNQWCNVVR WEFKHPTPFL RTREFLWQEG HTAFQSKDEA EDEVFKILDL YAQIYIDLLA IPVIKGRKTE KENFAGGDFT ATVEAYVPVN GRGIQGATSH HLGQNFSKMF NISFEDPNGG GKIYAWQNSW GISTRTIGAL VMIHGDNCGL VLPPRVATIQ MIIVPVGINA QTKDEQKTAL IEKAKEINNK LMDASIRAEL DIRDHISPGW KFNHWELKGV PVRIEIGPKD LANNQVTCVI RYSGEKRTIP IDGLASKCKD MLEEIHYSMY NRILEVRESH TKIVLEWNDF RSFLDQKFIL LSPFCGRIEC EDEIKKESMR GEENDPQAPA MGAKTLCIPL EQPEIPLPTN CINKNCPEKP QFFALFGRSY XMSDVESDFD EEAHAKLLKT INSLGNNAKK GPLIRKCPEK VRVKELVDLI HSTRNVDDVK KKLVEKKKKS NKAGKDEPRT LQAPSHRLVR ERIESTIAYS EIRKDLEEWA PIVKKNRLAE QLVFPLSKDP PIQRTASDLV LLFKPRTPLE LELAKLLKTS NNNLRDGEEY TEAELELIRA MSLKEAKAKW AQLKKMRALV GYREAKLKRQ AKIKSKTYHR HMKRQKRKKL IREFEEMMAK DPEAAKEKLE EIDRQRILER ATLKHRNGGK GVQQLARYAN KNKDFKKMYE EQIRLGRELA EKHGREKGSD SDSESENRDA VKLTSAEMLE RAAEVLEKEE QEKRSTKPEL KAKLYEMRKE KRETLRSSAL RATDAKFISN RKYDMNTDVA DESTACNEKV VELEREPEET IRSEEHSNKE QNQQNISENS GQDVEKKANK HSEKDLKNLK KKKSSKKMKN KKENKAAKDL DIDQLFDKAE KELAENALKE CERLKLEDEE NKRNQRHPPS DIRSIVIPQQ KEKKEITAKT EETVTDISLD PRHFLQIETM ALPQVSGDFV ETLEDTSHQT DDQEEIIAKA FEDVDVIGDF EAEKEAVEAA ENPKDIDLTL QGWGSWTGPG ITDRKKDKFI IKMPKKKRKD AGKTGLIISE AVDQSIEKLQ LKSVPFPYTT VEDYEAVIRQ PLGKEWNPQK IHRKLIQPQI VTKAGRIIKP LDKSILQDES EEEKI
NT Sequence
atggcattag tgaataatgg agatgacaca ttgggaatga aaattacttc acaaaaaaat gctatgcgaa agcttgaaaa taatctatct aaatctcata taagtaatag tacacctatg aaaggtcgag gggatacgaa gaaacaaact aaattgggca ttgaaatatc caaagatgag aattattccg aatggtatgt ccaggttata acgaaagcag aaatgatcga atattacgac atttctggtt gttatgtatt gcgaccgtgg tcttacgcta tttgggaatt tattcaggaa tggttcgatg aagaaattaa gaaattaggt gttaaaaatt gctattttcc attattcgtt tcgcaaagcg ctttggaaaa agagaagacg cacattagcg actttgctcc tgaggttgcg tggataacgc gagcaggaca gtcggatcta gctgaagcga ttgcaatacg tcctactagc gaaacagtaa tgtatcccag ctatgcaaaa tgggtacaat ctcatcgaga tcttccaatt aagctcaatc agtggtgtaa tgttgttcgt tgggaattca aacatccaac accattcttg agaactcgtg aatttttgtg gcaagaaggt catactgcat tccaatccaa agatgaagca gaagatgaag tattcaaaat acttgattta tatgctcaaa tctatatcga tttactggcc ataccagtta ttaaaggacg gaaaactgag aaagagaact ttgctggcgg agattttaca gctactgttg aggcatatgt gcctgtcaat ggacgtggga tacagggtgc tacatcgcat catttaggac aaaatttctc aaaaatgttt aacatatcgt ttgaagaccc aaacggtggc ggaaaaattt atgcatggca gaatagttgg ggtatatcaa ccagaacaat tggtgctctt gtgatgatac acggtgataa ttgtggtctg gtgcttcctc ctagagtagc tactatccag atgattattg ttcccgtagg aatcaacgct caaacaaagg atgaacaaaa aacagctcta attgaaaaag caaaagaaat caataacaag ttaatggatg caagtatacg tgctgagctt gatataaggg atcatatatc gcctggatgg aaattcaatc attgggaatt gaaaggtgtt cctgtaagaa tcgaaattgg tcccaaagat ttagccaata accaggtaac ttgcgtcatc agatattctg gcgagaaaag aaccattcct atcgatggtc ttgcttcaaa atgcaaagat atgctggagg aaattcacta ttccatgtat aataggatat tggaagttcg cgaatcgcac acaaagattg tattggaatg gaatgacttt cgaagttttc tagatcaaaa gtttattctg ctttcaccct tttgtggtcg tattgagtgc gaagatgaaa tcaaaaaaga aagcatgaga ggtgaggaaa atgatcctca agctcccgca atgggagcaa aaaccctctg cattccgctt gaacagcccg aaattccgct tccgactaac tgtatcaata aaaattgtcc agaaaaacca cagttttttg cactatttgg tcgaagttat tgaatgtcag atgtggagtc ggattttgat gaggaagccc atgcgaagtt gctgaagact atcaacagct tgggcaataa tgctaagaaa ggaccgttga tcaggaaatg tccggaaaaa gttcgggtta aggaacttgt tgatctgata cattctacaa gaaatgttga tgatgtaaag aagaaattgg tggagaagaa aaagaaatca aacaaagccg gaaaggacga accgagaact ttgcaagctc catctcacag actggttcgg gagaggattg aaagtaccat tgcatacagt gaaattcgaa aggatttaga agaatgggct ccaattgtta aaaagaatcg gcttgctgag caattagtat tcccacttag taaagaccca ccgatccagc gaacagcgtc ggatttagtg cttcttttca agccaaggac accactggaa ctagaattag ccaagttatt gaaaacatca aataataatc ttcgtgatgg cgaggaatat acagaagctg agttggaatt aataagggca atgagtctaa aagaggcaaa agcaaaatgg gctcaattga aaaaaatgcg ggctttggtt ggttaccgag aagctaaact gaaacgacaa gctaaaataa aaagcaaaac atatcatcga catatgaaac gccagaagag aaagaagtta atcagggaat ttgaagaaat gatggccaag gatccagaag ctgccaaaga gaaactggaa gaaattgatc gacagcgcat cctggaacga gctaccttaa agcatcgcaa cggcggaaaa ggtgtccaac agttggcaag atatgcgaat aaaaataagg atttcaaaaa aatgtacgaa gagcaaataa gattgggacg agaactcgcc gaaaagcatg gtcgtgagaa aggttcagat tctgattctg aatcggaaaa tagagatgca gtaaaactaa ctagcgctga aatgcttgag agagcagccg aagtgttaga gaaagaagaa caggaaaaga gatccactaa accagaattg aaagcgaaac tttacgaaat gagaaaagag aagcgggaga ctctcagaag tagtgcattg cgggccacag atgcgaagtt catatctaat cgaaagtatg atatgaatac tgatgtagcc gatgagtcga ctgcttgcaa tgaaaaagtt gtggaattgg aacgggaacc ggaagaaaca ataagaagtg aagagcattc gaacaaagag cagaatcagc aaaatatctc agaaaacagt ggacaagatg ttgaaaaaaa agctaacaaa cactccgaaa aagatctcaa aaatttgaaa aagaagaaaa gttccaagaa aatgaagaac aaaaaagaaa ataaagctgc aaaggacttg gatattgatc agttattcga taaagctgag aaagaacttg ctgaaaatgc tctaaaggag tgtgaacgtc taaaattaga ggacgaagaa aataagcgta atcagagaca tcctccgtct gacattagat ctatagtgat tccccaacaa aaagaaaaaa aagaaatcac tgcaaaaaca gaggaaacag tcacagatat ttctttggat ccaaggcatt ttctacaaat cgaaacaatg gcacttccac aagtctcggg cgactttgtt gaaactttag aagatactag tcaccaaaca gatgatcagg aagaaatcat agccaaggca ttcgaagatg ttgatgttat tggtgatttc gaagctgaaa aggaggccgt ggaagcggca gagaatccga aagatatcga tttaacgctt cagggatggg gttcatggac aggaccagga ataaccgata gaaaaaaaga caagtttata atcaaaatgc cgaaaaagaa gaggaaagat gcagggaaaa caggtttgat tatatcagaa gcagtagatc agtctattga aaaactccaa ctaaaatcgg tgccattccc ttatacaact gttgaggatt atgaagctgt aatacggcaa ccgttaggaa aggaatggaa tccacaaaaa attcatagga agcttattca accgcagatt gtaacaaaag cgggtcgaat aattaaacca ttggataaga gcatattgca agacgaatct gaagaagaaa aaatatga
Details for OnvoA.17194.a.B1.GE39189
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 5/6/2015 |
| EXPECTED MW: | 64kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA LVNNGDDTLG MKITSQKNAM RKLENNLSKS HISNSTPMKG RGDTKKQTKL GIEISKDENY SEWYVQVITK AEMIEYYDIS GCYVLRPWSY AIWEFIQEWF DEEIKKLGVK NCYFPLFVSQ SALEKEKTHI SDFAPEVAWI TRAGQSDLAE AIAIRPTSET VMYPSYAKWV QSHRDLPIKL NQWCNVVRWE FKHPTPFLRT REFLWQEGHT AFQSKDEAED EVFKILDLYA QIYIDLLAIP VIKGRKTEKE NFAGGDFTAT VEAYVPVNGR GIQGATSHHL GQNFSKMFNI SFEDPNGGGK IYAWQNSWGI STRTIGALVM IHGDNCGLVL PPRVATIQMI IVPVGINAQT KDEQKTALIE KAKEINNKLM DASIRAELDI RDHISPGWKF NHWELKGVPV RIEIGPKDLA NNQVTCVIRY SGEKRTIPID GLASKCKDML EEIHYSMYNR ILEVRESHTK IVLEWNDFRS FLDQKFILLS PFCGRIECED EIKKESMRGE ENDPQAPAMG AKTLCIPLEQ PEIPLPTNCI NKNCPEKPQF FALFGRSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcat tagtgaataa tggagatgac acattgggaa tgaaaattac ttcacaaaaa aatgctatgc gaaagcttga aaataatcta tctaaatctc atataagtaa tagtacacct atgaaaggtc gaggggatac gaagaaacaa actaaattgg gcattgaaat atccaaagat gagaattatt ccgaatggta tgtccaggtt ataacgaaag cagaaatgat cgaatattac gacatttctg gttgttatgt attgcgaccg tggtcttacg ctatttggga atttattcag gaatggttcg atgaagaaat taagaaatta ggtgttaaaa attgctattt tccattattc gtttcgcaaa gcgctttgga aaaagagaag acgcacatta gcgactttgc tcctgaggtt gcgtggataa cgcgagcagg acagtcggat ctagctgaag cgattgcaat acgtcctact agcgaaacag taatgtatcc cagctatgca aaatgggtac aatctcatcg agatcttcca attaagctca atcagtggtg taatgttgtt cgttgggaat tcaaacatcc aacaccattc ttgagaactc gtgaattttt gtggcaagaa ggtcatactg cattccaatc caaagatgaa gcagaagatg aagtattcaa aatacttgat ttatatgctc aaatctatat cgatttactg gccataccag ttattaaagg acggaaaact gagaaagaga actttgctgg cggagatttt acagctactg ttgaggcata tgtgcctgtc aatggacgtg ggatacaggg tgctacatcg catcatttag gacaaaattt ctcaaaaatg tttaacatat cgtttgaaga cccaaacggt ggcggaaaaa tttatgcatg gcagaatagt tggggtatat caaccagaac aattggtgct cttgtgatga tacacggtga taattgtggt ctggtgcttc ctcctagagt agctactatc cagatgatta ttgttcccgt aggaatcaac gctcaaacaa aggatgaaca aaaaacagct ctaattgaaa aagcaaaaga aatcaataac aagttaatgg atgcaagtat acgtgctgag cttgatataa gggatcatat atcgcctgga tggaaattca atcattggga attgaaaggt gttcctgtaa gaatcgaaat tggtcccaaa gatttagcca ataaccaggt aacttgcgtc atcagatatt ctggcgagaa aagaaccatt cctatcgatg gtcttgcttc aaaatgcaaa gatatgctgg aggaaattca ctattccatg tataatagga tattggaagt tcgcgaatcg cacacaaaga ttgtattgga atggaatgac tttcgaagtt ttctagatca aaagtttatt ctgctttcac ccttttgtgg tcgtattgag tgcgaagatg aaatcaaaaa agaaagcatg agaggtgagg aaaatgatcc tcaagctccc gcaatgggag caaaaaccct ctgcattccg cttgaacagc ccgaaattcc gcttccgact aactgtatca ataaaaattg tccagaaaaa ccacagtttt ttgcactatt tggtcgaagt tattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for OnvoA.17194.a.B2.GE40762
| HARVESTED ON: | 11/21/2016 |
| SEQUENCED ON: | 11/22/2016 |
| EXPECTED MW: | 56kDa |
| OBSERVED MW: | 56kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMK DENYSEWYVQ VITKAEMIEY YDISGCYVLR PWSYAIWEFI QEWFDEEIKK LGVKNCYFPL FVSQSALEKE KTHISDFAPE VAWITRAGQS DLAEAIAIRP TSETVMYPSY AKWVQSHRDL PIKLNQWCNV VRWEFKHPTP FLRTREFLWQ EGHTAFQSKD EAEDEVFKIL DLYAQIYIDL LAIPVIKGRK TEKENFAGGD FTATVEAYVP VNGRGIQGAT SHHLGQNFSK MFNISFEDPN GGGKIYAWQN SWGISTRTIG ALVMIHGDNC GLVLPPRVAT IQMIIVPVGI NAQTKDEQKT ALIEKAKEIN NKLMDASIRA ELDIRDHISP GWKFNHWELK GVPVRIEIGP KDLANNQVTC VIRYSGEKRT IPIDGLASKC KDMLEEIHYS MYNRILEVRE SHTKIVLEWN DFRSFLDQKF ILLSPFCGRI ECEDEIKKES MRGEENDPQA PAMGAKTLCI PLEQPEIPLP
Validated NT Sequence
agtcggaagc ggaatttcgg gctgttcaag cggaatgcag agggtttttg ctcccattgc gggagcttga ggatcatttt cctcacctct catgctttct tttttgattt catcttcgca ctcaatacga ccacaaaagg gtgaaagcag aataaacttt tgatctagaa aacttcgaaa gtcattccat tccaatacaa tctttgtgtg cgattcgcga acttccaata tcctattata catggaatag tgaatttcct ccagcatatc tttgcatttt gaagcaagac catcgatagg aatggttctt ttctcgccag aatatctgat gacgcaagtt acctggttat tggctaaatc tttgggacca atttcgattc ttacaggaac acctttcaat tcccaatgat tgaatttcca tccaggcgat atatgatccc ttatatcaag ctcagcacgt atacttgcat ccattaactt gttattgatt tcttttgctt tttcaattag agctgttttt tgttcatcct ttgtttgagc gttgattcct acgggaacaa taatcatctg gatagtagct actctaggag gaagcaccag accacaatta tcaccgtgta tcatcacaag agcaccaatt gttctggttg atatacccca actattctgc catgcataaa tttttccgcc accgtttggg tcttcaaacg atatgttaaa catttttgag aaattttgtc ctaaatgatg cgatgtagca ccctgtatcc cacgtccatt gacaggcaca tatgcctcaa cagtagctgt aaaatctccg ccagcaaagt tctctttctc agttttccgt cctttaataa ctggtatggc cagtaaatcg atatagattt gagcatataa atcaagtatt ttgaatactt catcttctgc ttcatctttg gattggaatg cagtatgacc ttcttgccac aaaaattcac gagttctcaa gaatggtgtt ggatgtttga attcccaacg aacaacatta caccactgat tgagcttaat tggaagatct cgatgagatt gtacccattt tgcatagctg ggatacatta ctgtttcgct agtaggacgt attgcaatcg cttcagctag atccgactgt cctgctcgcg ttatccacgc aacctcagga gcaaagtcgc taatgtgcgt cttctctttt tccaaagcgc tttgcgaaac gaataatgga aaatagcaat ttttaacacc taatttctta atttcttcat cgaaccattc ctgaataaat tcccaaatag cgtaagacca cggtcgcaat acataacaac cagaaatgtc gtaatattcg atcatttctg ctttcgttat aacctggaca taccattcgg aataattctc atctttcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHKD ENYSEWYVQV ITKAEMIEYY DISGCYVLRP WSYAIWEFIQ EWFDEEIKKL GVKNCYFPLF VSQSALEKEK THISDFAPEV AWITRAGQSD LAEAIAIRPT SETVMYPSYA KWVQSHRDLP IKLNQWCNVV RWEFKHPTPF LRTREFLWQE GHTAFQSKDE AEDEVFKILD LYAQIYIDLL AIPVIKGRKT EKENFAGGDF TATVEAYVPV NGRGIQGATS HHLGQNFSKM FNISFEDPNG GGKIYAWQNS WGISTRTIGA LVMIHGDNCG LVLPPRVATI QMIIVPVGIN AQTKDEQKTA LIEKAKEINN KLMDASIRAE LDIRDHISPG WKFNHWELKG VPVRIEIGPK DLANNQVTCV IRYSGEKRTI PIDGLASKCK DMLEEIHYSM YNRILEVRES HTKIVLEWND FRSFLDQKFI LLSPFCGRIE CEDEIKKESM RGEENDPQAP AMGAKTLCIP LEQPEIPLPT NCINKNC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaagatg agaattattc cgaatggtat gtccaggtta taacgaaagc agaaatgatc gaatattacg acatttctgg ttgttatgta ttgcgaccgt ggtcttacgc tatttgggaa tttattcagg aatggttcga tgaagaaatt aagaaattag gtgttaaaaa ttgctatttt ccattattcg tttcgcaaag cgctttggaa aaagagaaga cgcacattag cgactttgct cctgaggttg cgtggataac gcgagcagga cagtcggatc tagctgaagc gattgcaata cgtcctacta gcgaaacagt aatgtatccc agctatgcaa aatgggtaca atctcatcga gatcttccaa ttaagctcaa tcagtggtgt aatgttgttc gttgggaatt caaacatcca acaccattct tgagaactcg tgaatttttg tggcaagaag gtcatactgc attccaatcc aaagatgaag cagaagatga agtattcaaa atacttgatt tatatgctca aatctatatc gatttactgg ccataccagt tattaaagga cggaaaactg agaaagagaa ctttgctggc ggagatttta cagctactgt tgaggcatat gtgcctgtca atggacgtgg gatacagggt gctacatcgc atcatttagg acaaaatttc tcaaaaatgt ttaacatatc gtttgaagac ccaaacggtg gcggaaaaat ttatgcatgg cagaatagtt ggggtatatc aaccagaaca attggtgctc ttgtgatgat acacggtgat aattgtggtc tggtgcttcc tcctagagta gctactatcc agatgattat tgttcccgta ggaatcaacg ctcaaacaaa ggatgaacaa aaaacagctc taattgaaaa agcaaaagaa atcaataaca agttaatgga tgcaagtata cgtgctgagc ttgatataag ggatcatata tcgcctggat ggaaattcaa tcattgggaa ttgaaaggtg ttcctgtaag aatcgaaatt ggtcccaaag atttagccaa taaccaggta acttgcgtca tcagatattc tggcgagaaa agaaccattc ctatcgatgg tcttgcttca aaatgcaaag atatgctgga ggaaattcac tattccatgt ataataggat attggaagtt cgcgaatcgc acacaaagat tgtattggaa tggaatgact ttcgaagttt tctagatcaa aagtttattc tgctttcacc cttttgtggt cgtattgagt gcgaagatga aatcaaaaaa gaaagcatga gaggtgagga aaatgatcct caagctcccg caatgggagc aaaaaccctc tgcattccgc ttgaacagcc cgaaattccg cttccgacta actgtatcaa taaaaattgt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for OnvoA.17194.a.B5.GE40763
| HARVESTED ON: | 11/21/2016 |
| SEQUENCED ON: | 11/22/2016 |
| EXPECTED MW: | 58kDa |
| OBSERVED MW: | 58kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMI EISKDENYSE WYVQVITKAE MIEYYDISGC YVLRPWSYAI WEFIQEWFDE EIKKLGVKNC YFPLFVSQSA LEKEKTHISD FAPEVAWITR AGQSDLAEAI AIRPTSETVM YPSYAKWVQS HRDLPIKLNQ WCNVVRWEFK HPTPFLRTRE FLWQEGHTAF QSKDEAEDEV FKILDLYAQI YIDLLAIPVI KGRKTEKENF AGGDFTATVE AYVPVNGRGI QGATSHHLGQ NFSKMFNISF EDPNGGGKIY AWQNSWGIST RTIGALVMIH GDNCGLVLPP RVATIQMIIV PVGINAQTKD EQKTALIEKA KEINNKLMDA SIRAELDIRD HISPGWKFNH WELKGVPVRI EIGPKDLANN QVTCVIRYSG EKRTIPIDGL ASKCKDMLEE IHYSMYNRIL EVRESHTKIV LEWNDFRSFL DQKFILLSPF CGRIECEDEI KKESMRGEEN DPQAPAMGAK TLCIPLEQPE IPLPTNCINK NCPEKPQFFA LFGRSY
Validated NT Sequence
atggctcacc accaccacca ccatatgatt gaaatatcca aagatgagaa ttattccgaa tggtatgtcc aggttataac gaaagcagaa atgatcgaat attacgacat ttctggttgt tatgtattgc gaccgtggtc ttacgctatt tgggaattta ttcaggaatg gttcgatgaa gaaattaaga aattaggtgt taaaaattgc tattttccat tattcgtttc gcaaagcgct ttggaaaaag agaagacgca cattagcgac tttgctcctg aggttgcgtg gataacgcga gcaggacagt cggatctagc tgaagcgatt gcaatacgtc ctactagcga aacagtaatg tatcccagct atgcaaaatg ggtacaatct catcgagatc ttccaattaa gctcaatcag tggtgtaatg ttgttcgttg ggaattcaaa catccaacac cattcttgag aactcgtgaa tttttgtggc aagaaggtca tactgcattc caatccaaag atgaagcaga agatgaagta ttcaaaatac ttgatttata tgctcaaatc tatatcgatt tactggccat accagttatt aaaggacgga aaactgagaa agagaacttt gctggcggag attttacagc tactgttgag gcatatgtgc ctgtcaatgg acgtgggata cagggtgcta catcgcatca tttaggacaa aatttctcaa aaatgtttaa catatcgttt gaagacccaa acggtggcgg aaaaatttat gcatggcaga atagttgggg tatatcaacc agaacaattg gtgctcttgt gatgatacac ggtgataatt gtggtctggt gcttcctcct agagtagcta ctatccagat gattattgtt cccgtaggaa tcaacgctca aacaaaggat gaacaaaaaa cagctctaat tgaaaaagca aaagaaatca ataacaagtt aatggatgca agtatacgtg ctgagcttga tataagggat catatatcgc ctggatggaa attcaatcat tgggaattga aaggtgttcc tgtaagaatc gaaattggtc ccaaagattt agccaataac caggtaactt gcgtcatcag atattctggc gagaaaagaa ccattcctat cgatggtctt gcttcaaaat gcaaagatat gctggaggaa attcactatt ccatgtataa taggatattg gaagttcgcg aatcgcacac aaagattgta ttggaatgga atgactttcg aagttttcta gatcaaaagt ttattctgct ttcacccttt tgtggtcgta ttgagtgcga agatgaaatc aaaaaagaaa gcatgagagg tgaggaaaat gatcctcaag ctcccgcaat gggagcaaaa accctctgca ttccgcttga acagcccgaa attccgcttc cgactaactg tatcaataaa aattgtccag aaaaaccaca gttttttgca ctatttggtc gaagttatta a
Expected Protein Sequence
MAHHHHHHIE ISKDENYSEW YVQVITKAEM IEYYDISGCY VLRPWSYAIW EFIQEWFDEE IKKLGVKNCY FPLFVSQSAL EKEKTHISDF APEVAWITRA GQSDLAEAIA IRPTSETVMY PSYAKWVQSH RDLPIKLNQW CNVVRWEFKH PTPFLRTREF LWQEGHTAFQ SKDEAEDEVF KILDLYAQIY IDLLAIPVIK GRKTEKENFA GGDFTATVEA YVPVNGRGIQ GATSHHLGQN FSKMFNISFE DPNGGGKIYA WQNSWGISTR TIGALVMIHG DNCGLVLPPR VATIQMIIVP VGINAQTKDE QKTALIEKAK EINNKLMDAS IRAELDIRDH ISPGWKFNHW ELKGVPVRIE IGPKDLANNQ VTCVIRYSGE KRTIPIDGLA SKCKDMLEEI HYSMYNRILE VRESHTKIVL EWNDFRSFLD QKFILLSPFC GRIECEDEIK KESMRGEEND PQAPAMGAKT LCIPLEQPEI PLPTNCINKN CPEKPQFFAL FGRSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattgaaa tatccaaaga tgagaattat tccgaatggt atgtccaggt tataacgaaa gcagaaatga tcgaatatta cgacatttct ggttgttatg tattgcgacc gtggtcttac gctatttggg aatttattca ggaatggttc gatgaagaaa ttaagaaatt aggtgttaaa aattgctatt ttccattatt cgtttcgcaa agcgctttgg aaaaagagaa gacgcacatt agcgactttg ctcctgaggt tgcgtggata acgcgagcag gacagtcgga tctagctgaa gcgattgcaa tacgtcctac tagcgaaaca gtaatgtatc ccagctatgc aaaatgggta caatctcatc gagatcttcc aattaagctc aatcagtggt gtaatgttgt tcgttgggaa ttcaaacatc caacaccatt cttgagaact cgtgaatttt tgtggcaaga aggtcatact gcattccaat ccaaagatga agcagaagat gaagtattca aaatacttga tttatatgct caaatctata tcgatttact ggccatacca gttattaaag gacggaaaac tgagaaagag aactttgctg gcggagattt tacagctact gttgaggcat atgtgcctgt caatggacgt gggatacagg gtgctacatc gcatcattta ggacaaaatt tctcaaaaat gtttaacata tcgtttgaag acccaaacgg tggcggaaaa atttatgcat ggcagaatag ttggggtata tcaaccagaa caattggtgc tcttgtgatg atacacggtg ataattgtgg tctggtgctt cctcctagag tagctactat ccagatgatt attgttcccg taggaatcaa cgctcaaaca aaggatgaac aaaaaacagc tctaattgaa aaagcaaaag aaatcaataa caagttaatg gatgcaagta tacgtgctga gcttgatata agggatcata tatcgcctgg atggaaattc aatcattggg aattgaaagg tgttcctgta agaatcgaaa ttggtcccaa agatttagcc aataaccagg taacttgcgt catcagatat tctggcgaga aaagaaccat tcctatcgat ggtcttgctt caaaatgcaa agatatgctg gaggaaattc actattccat gtataatagg atattggaag ttcgcgaatc gcacacaaag attgtattgg aatggaatga ctttcgaagt tttctagatc aaaagtttat tctgctttca cccttttgtg gtcgtattga gtgcgaagat gaaatcaaaa aagaaagcat gagaggtgag gaaaatgatc ctcaagctcc cgcaatggga gcaaaaaccc tctgcattcc gcttgaacag cccgaaattc cgcttccgac taactgtatc aataaaaatt gtccagaaaa accacagttt tttgcactat ttggtcgaag ttattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for OnvoA.17194.a.MB1.GE39288
| HARVESTED ON: | 6/25/2015 |
| SEQUENCED ON: | data unavailable |
| EXPECTED MW: | 108kDa |
| OBSERVED MW: | 94kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MALVNNGDDT LGMKITSQKN AMRKLENNLS KSHISNSTPM KGRGDTKKQT KLGIEISKDE NYSEWYVQVI TKAEMIEYYD ISGCYVLRPW SYAIWEFIQE WFDEEIKKLG VKNCYFPLFV SQSALEKEKT HISDFAPEVA WITRAGQSDL AEAIAIRPTS ETVMYPSYAK WVQSHRDLPI KLNQWCNVVR WEFKHPTPFL RTREFLWQEG HTAFQSKDEA EDEVFKILDL YAQIYIDLLA IPVIKGRKTE KENFAGGDFT ATVEAYVPVN GRGIQGATSH HLGQNFSKMF NISFEDPNGG GKIYAWQNSW GISTRTIGAL VMIHGDNCGL VLPPRVATIQ MIIVPVGINA QTKDEQKTAL IEKAKEINNK LMDASIRAEL DIRDHISPGW KFNHWELKGV PVRIEIGPKD LANNQVTCVI RYSGEKRTIP IDGLASKCKD MLEEIHYSMY NRILEVRESH TKIVLEWNDF RSFLDQKFIL LSPFCGRIEC EDEIKKESMR GEENDPQAPA MGAKTLCIPL EQPEIPLPTN CINKNCPEKP QFFALFGRSY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggc attagtgaat aatggagatg acacattggg aatgaaaatt acttcacaaa aaaatgctat gcgaaagctt gaaaataatc tatctaaatc tcatataagt aatagtacac ctatgaaagg tcgaggggat acgaagaaac aaactaaatt gggcattgaa atatccaaag atgagaatta ttccgaatgg tatgtccagg ttataacgaa agcagaaatg atcgaatatt acgacatttc tggttgttat gtattgcgac cgtggtctta cgctatttgg gaatttattc aggaatggtt cgatgaagaa attaagaaat taggtgttaa aaattgctat tttccattat tcgtttcgca aagcgctttg gaaaaagaga agacgcacat tagcgacttt gctcctgagg ttgcgtggat aacgcgagca ggacagtcgg atctagctga agcgattgca atacgtccta ctagcgaaac agtaatgtat cccagctatg caaaatgggt acaatctcat cgagatcttc caattaagct caatcagtgg tgtaatgttg ttcgttggga attcaaacat ccaacaccat tcttgagaac tcgtgaattt ttgtggcaag aaggtcatac tgcattccaa tccaaagatg aagcagaaga tgaagtattc aaaatacttg atttatatgc tcaaatctat atcgatttac tggccatacc agttattaaa ggacggaaaa ctgagaaaga gaactttgct ggcggagatt ttacagctac tgttgaggca tatgtgcctg tcaatggacg tgggatacag ggtgctacat cgcatcattt aggacaaaat ttctcaaaaa tgtttaacat atcgtttgaa gacccaaacg gtggcggaaa aatttatgca tggcagaata gttggggtat atcaaccaga acaattggtg ctcttgtgat gatacacggt gataattgtg gtctggtgct tcctcctaga gtagctacta tccagatgat tattgttccc gtaggaatca acgctcaaac aaaggatgaa caaaaaacag ctctaattga aaaagcaaaa gaaatcaata acaagttaat ggatgcaagt atacgtgctg agcttgatat aagggatcat atatcgcctg gatggaaatt caatcattgg gaattgaaag gtgttcctgt aagaatcgaa attggtccca aagatttagc caataaccag gtaacttgcg tcatcagata ttctggcgag aaaagaacca ttcctatcga tggtcttgct tcaaaatgca aagatatgct ggaggaaatt cactattcca tgtataatag gatattggaa gttcgcgaat cgcacacaaa gattgtattg gaatggaatg actttcgaag ttttctagat caaaagttta ttctgctttc acccttttgt ggtcgtattg agtgcgaaga tgaaatcaaa aaagaaagca tgagaggtga ggaaaatgat cctcaagctc ccgcaatggg agcaaaaacc ctctgcattc cgcttgaaca gcccgaaatt ccgcttccga ctaactgtat caataaaaat tgtccagaaa aaccacagtt ttttgcacta tttggtcgaa gttattaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for OnvoA.17194.a.MB2.GE39289
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | data unavailable |
| EXPECTED MW: | 100kDa |
| OBSERVED MW: | 46kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 27 |
| PERCENT COVERAGE: | 9 |
Validated AA Sequence
MLRIIDTETC GLQGGIVEIA SVDVIDGKIV NPMSHLVRPD RPISPQAMAI HRITEAMVAD KPWIEDVIPH YXXXEXXXXX XXXXX
Validated NT Sequence
taacaatttt tattgataca gttagtcggt cagcgttcat cacagggttg gcaaggcgat atcattccat tacgctaggc tttttcggcc tggagcatgc catgttgcgc attatcgata cagaaacctg cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg gaaaaatcgt caaccccatg agccacctgg tgcgccccga tcgtcctatt agtccacaag cgatggcgat tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg tgatcccaca ctattannnn nntgaannnn nnnnnnnnnn nnncnnnnnn nnnnncc
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS KDENYSEWYV QVITKAEMIE YYDISGCYVL RPWSYAIWEF IQEWFDEEIK KLGVKNCYFP LFVSQSALEK EKTHISDFAP EVAWITRAGQ SDLAEAIAIR PTSETVMYPS YAKWVQSHRD LPIKLNQWCN VVRWEFKHPT PFLRTREFLW QEGHTAFQSK DEAEDEVFKI LDLYAQIYID LLAIPVIKGR KTEKENFAGG DFTATVEAYV PVNGRGIQGA TSHHLGQNFS KMFNISFEDP NGGGKIYAWQ NSWGISTRTI GALVMIHGDN CGLVLPPRVA TIQMIIVPVG INAQTKDEQK TALIEKAKEI NNKLMDASIR AELDIRDHIS PGWKFNHWEL KGVPVRIEIG PKDLANNQVT CVIRYSGEKR TIPIDGLASK CKDMLEEIHY SMYNRILEVR ESHTKIVLEW NDFRSFLDQK FILLSPFCGR IECEDEIKKE SMRGEENDPQ APAMGAKTLC IPLEQPEIPL PTNCINKNC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgaaaga tgagaattat tccgaatggt atgtccaggt tataacgaaa gcagaaatga tcgaatatta cgacatttct ggttgttatg tattgcgacc gtggtcttac gctatttggg aatttattca ggaatggttc gatgaagaaa ttaagaaatt aggtgttaaa aattgctatt ttccattatt cgtttcgcaa agcgctttgg aaaaagagaa gacgcacatt agcgactttg ctcctgaggt tgcgtggata acgcgagcag gacagtcgga tctagctgaa gcgattgcaa tacgtcctac tagcgaaaca gtaatgtatc ccagctatgc aaaatgggta caatctcatc gagatcttcc aattaagctc aatcagtggt gtaatgttgt tcgttgggaa ttcaaacatc caacaccatt cttgagaact cgtgaatttt tgtggcaaga aggtcatact gcattccaat ccaaagatga agcagaagat gaagtattca aaatacttga tttatatgct caaatctata tcgatttact ggccatacca gttattaaag gacggaaaac tgagaaagag aactttgctg gcggagattt tacagctact gttgaggcat atgtgcctgt caatggacgt gggatacagg gtgctacatc gcatcattta ggacaaaatt tctcaaaaat gtttaacata tcgtttgaag acccaaacgg tggcggaaaa atttatgcat ggcagaatag ttggggtata tcaaccagaa caattggtgc tcttgtgatg atacacggtg ataattgtgg tctggtgctt cctcctagag tagctactat ccagatgatt attgttcccg taggaatcaa cgctcaaaca aaggatgaac aaaaaacagc tctaattgaa aaagcaaaag aaatcaataa caagttaatg gatgcaagta tacgtgctga gcttgatata agggatcata tatcgcctgg atggaaattc aatcattggg aattgaaagg tgttcctgta agaatcgaaa ttggtcccaa agatttagcc aataaccagg taacttgcgt catcagatat tctggcgaga aaagaaccat tcctatcgat ggtcttgctt caaaatgcaa agatatgctg gaggaaattc actattccat gtataatagg atattggaag ttcgcgaatc gcacacaaag attgtattgg aatggaatga ctttcgaagt tttctagatc aaaagtttat tctgctttca cccttttgtg gtcgtattga gtgcgaagat gaaatcaaaa aagaaagcat gagaggtgag gaaaatgatc ctcaagctcc cgcaatggga gcaaaaaccc tctgcattcc gcttgaacag cccgaaattc cgcttccgac taactgtatc aataaaaatt gttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for OnvoA.17194.a.MB1.PS02533
| PURIFICATION DATe: | 11/5/2015 |
| CONCENTRATION: | 43mg/ml |
| OBSERVED MW: | 95kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MALVNNGDDT LGMKITSQKN AMRKLENNLS KSHISNSTPM KGRGDTKKQT KLGIEISKDE NYSEWYVQVI TKAEMIEYYD ISGCYVLRPW SYAIWEFIQE WFDEEIKKLG VKNCYFPLFV SQSALEKEKT HISDFAPEVA WITRAGQSDL AEAIAIRPTS ETVMYPSYAK WVQSHRDLPI KLNQWCNVVR WEFKHPTPFL RTREFLWQEG HTAFQSKDEA EDEVFKILDL YAQIYIDLLA IPVIKGRKTE KENFAGGDFT ATVEAYVPVN GRGIQGATSH HLGQNFSKMF NISFEDPNGG GKIYAWQNSW GISTRTIGAL VMIHGDNCGL VLPPRVATIQ MIIVPVGINA QTKDEQKTAL IEKAKEINNK LMDASIRAEL DIRDHISPGW KFNHWELKGV PVRIEIGPKD LANNQVTCVI RYSGEKRTIP IDGLASKCKD MLEEIHYSMY NRILEVRESH TKIVLEWNDF RSFLDQKFIL LSPFCGRIEC EDEIKKESMR GEENDPQAPA MGAKTLCIPL EQPEIPLPTN CINKNCPEKP QFFALFGRSY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCACA TGAAAATCGA AGAAGGTAAA CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT AATTCGAGCT CGAACAACAA CAACAATAAC AATAACAACA ACCTCGGGAT CGAGGGAAGG ATTTCAGAAG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGATGGC ATTAGTGAAT AATGGAGATG ACACATTGGG AATGAAAATT ACTTCACAAA AAAATGCTAT GCGAAAGCTT GAAAATAATC TATCTAAATC TCATATAAGT AATAGTACAC CTATGAAAGG TCGAGGGGAT ACGAAGAAAC AAACTAAATT GGGCATTGAA ATATCCAAAG ATGAGAATTA TTCCGAATGG TATGTCCAGG TTATAACGAA AGCAGAAATG ATCGAATATT ACGACATTTC TGGTTGTTAT GTATTGCGAC CGTGGTCTTA CGCTATTTGG GAATTTATTC AGGAATGGTT CGATGAAGAA ATTAAGAAAT TAGGTGTTAA AAATTGCTAT TTTCCATTAT TCGTTTCGCA AAGCGCTTTG GAAAAAGAGA AGACGCACAT TAGCGACTTT GCTCCTGAGG TTGCGTGGAT AACGCGAGCA GGACAGTCGG ATCTAGCTGA AGCGATTGCA ATACGTCCTA CTAGCGAAAC AGTAATGTAT CCCAGCTATG CAAAATGGGT ACAATCTCAT CGAGATCTTC CAATTAAGCT CAATCAGTGG TGTAATGTTG TTCGTTGGGA ATTCAAACAT CCAACACCAT TCTTGAGAAC TCGTGAATTT TTGTGGCAAG AAGGTCATAC TGCATTCCAA TCCAAAGATG AAGCAGAAGA TGAAGTATTC AAAATACTTG ATTTATATGC TCAAATCTAT ATCGATTTAC TGGCCATACC AGTTATTAAA GGACGGAAAA CTGAGAAAGA GAACTTTGCT GGCGGAGATT TTACAGCTAC TGTTGAGGCA TATGTGCCTG TCAATGGACG TGGGATACAG GGTGCTACAT CGCATCATTT AGGACAAAAT TTCTCAAAAA TGTTTAACAT ATCGTTTGAA GACCCAAACG GTGGCGGAAA AATTTATGCA TGGCAGAATA GTTGGGGTAT ATCAACCAGA ACAATTGGTG CTCTTGTGAT GATACACGGT GATAATTGTG GTCTGGTGCT TCCTCCTAGA GTAGCTACTA TCCAGATGAT TATTGTTCCC GTAGGAATCA ACGCTCAAAC AAAGGATGAA CAAAAAACAG CTCTAATTGA AAAAGCAAAA GAAATCAATA ACAAGTTAAT GGATGCAAGT ATACGTGCTG AGCTTGATAT AAGGGATCAT ATATCGCCTG GATGGAAATT CAATCATTGG GAATTGAAAG GTGTTCCTGT AAGAATCGAA ATTGGTCCCA AAGATTTAGC CAATAACCAG GTAACTTGCG TCATCAGATA TTCTGGCGAG AAAAGAACCA TTCCTATCGA TGGTCTTGCT TCAAAATGCA AAGATATGCT GGAGGAAATT CACTATTCCA TGTATAATAG GATATTGGAA GTTCGCGAAT CGCACACAAA GATTGTATTG GAATGGAATG ACTTTCGAAG TTTTCTAGAT CAAAAGTTTA TTCTGCTTTC ACCCTTTTGT GGTCGTATTG AGTGCGAAGA TGAAATCAAA AAAGAAAGCA TGAGAGGTGA GGAAAATGAT CCTCAAGCTC CCGCAATGGG AGCAAAAACC CTCTGCATTC CGCTTGAACA GCCCGAAATT CCGCTTCCGA CTAACTGTAT CAATAAAAAT TGTCCAGAAA AACCACAGTT TTTTGCACTA TTTGGTCGAA GTTATTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for OnvoA.17194.a.B5.PW38185
| PURIFICATION DATe: | 1/30/2017 |
| CONCENTRATION: | 17mg/ml |
| OBSERVED MW: | 65kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI EISKDENYSE WYVQVITKAE MIEYYDISGC YVLRPWSYAI WEFIQEWFDE EIKKLGVKNC YFPLFVSQSA LEKEKTHISD FAPEVAWITR AGQSDLAEAI AIRPTSETVM YPSYAKWVQS HRDLPIKLNQ WCNVVRWEFK HPTPFLRTRE FLWQEGHTAF QSKDEAEDEV FKILDLYAQI YIDLLAIPVI KGRKTEKENF AGGDFTATVE AYVPVNGRGI QGATSHHLGQ NFSKMFNISF EDPNGGGKIY AWQNSWGIST RTIGALVMIH GDNCGLVLPP RVATIQMIIV PVGINAQTKD EQKTALIEKA KEINNKLMDA SIRAELDIRD HISPGWKFNH WELKGVPVRI EIGPKDLANN QVTCVIRYSG EKRTIPIDGL ASKCKDMLEE IHYSMYNRIL EVRESHTKIV LEWNDFRSFL DQKFILLSPF CGRIECEDEI KKESMRGEEN DPQAPAMGAK TLCIPLEQPE IPLPTNCINK NCPEKPQFFA LFGRSY
Validated NT Sequence
atggctcacc accaccacca ccatatgatt gaaatatcca aagatgagaa ttattccgaa tggtatgtcc aggttataac gaaagcagaa atgatcgaat attacgacat ttctggttgt tatgtattgc gaccgtggtc ttacgctatt tgggaattta ttcaggaatg gttcgatgaa gaaattaaga aattaggtgt taaaaattgc tattttccat tattcgtttc gcaaagcgct ttggaaaaag agaagacgca cattagcgac tttgctcctg aggttgcgtg gataacgcga gcaggacagt cggatctagc tgaagcgatt gcaatacgtc ctactagcga aacagtaatg tatcccagct atgcaaaatg ggtacaatct catcgagatc ttccaattaa gctcaatcag tggtgtaatg ttgttcgttg ggaattcaaa catccaacac cattcttgag aactcgtgaa tttttgtggc aagaaggtca tactgcattc caatccaaag atgaagcaga agatgaagta ttcaaaatac ttgatttata tgctcaaatc tatatcgatt tactggccat accagttatt aaaggacgga aaactgagaa agagaacttt gctggcggag attttacagc tactgttgag gcatatgtgc ctgtcaatgg acgtgggata cagggtgcta catcgcatca tttaggacaa aatttctcaa aaatgtttaa catatcgttt gaagacccaa acggtggcgg aaaaatttat gcatggcaga atagttgggg tatatcaacc agaacaattg gtgctcttgt gatgatacac ggtgataatt gtggtctggt gcttcctcct agagtagcta ctatccagat gattattgtt cccgtaggaa tcaacgctca aacaaaggat gaacaaaaaa cagctctaat tgaaaaagca aaagaaatca ataacaagtt aatggatgca agtatacgtg ctgagcttga tataagggat catatatcgc ctggatggaa attcaatcat tgggaattga aaggtgttcc tgtaagaatc gaaattggtc ccaaagattt agccaataac caggtaactt gcgtcatcag atattctggc gagaaaagaa ccattcctat cgatggtctt gcttcaaaat gcaaagatat gctggaggaa attcactatt ccatgtataa taggatattg gaagttcgcg aatcgcacac aaagattgta ttggaatgga atgactttcg aagttttcta gatcaaaagt ttattctgct ttcacccttt tgtggtcgta ttgagtgcga agatgaaatc aaaaaagaaa gcatgagagg tgaggaaaat gatcctcaag ctcccgcaat gggagcaaaa accctctgca ttccgcttga acagcccgaa attccgcttc cgactaactg tatcaataaa aattgtccag aaaaaccaca gttttttgca ctatttggtc gaagttatta a
Expressed Protein Sequence
MAHHHHHHIE ISKDENYSEW YVQVITKAEM IEYYDISGCY VLRPWSYAIW EFIQEWFDEE IKKLGVKNCY FPLFVSQSAL EKEKTHISDF APEVAWITRA GQSDLAEAIA IRPTSETVMY PSYAKWVQSH RDLPIKLNQW CNVVRWEFKH PTPFLRTREF LWQEGHTAFQ SKDEAEDEVF KILDLYAQIY IDLLAIPVIK GRKTEKENFA GGDFTATVEA YVPVNGRGIQ GATSHHLGQN FSKMFNISFE DPNGGGKIYA WQNSWGISTR TIGALVMIHG DNCGLVLPPR VATIQMIIVP VGINAQTKDE QKTALIEKAK EINNKLMDAS IRAELDIRDH ISPGWKFNHW ELKGVPVRIE IGPKDLANNQ VTCVIRYSGE KRTIPIDGLA SKCKDMLEEI HYSMYNRILE VRESHTKIVL EWNDFRSFLD QKFILLSPFC GRIECEDEIK KESMRGEEND PQAPAMGAKT LCIPLEQPEI PLPTNCINKN CPEKPQFFAL FGRSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATTGAAA TATCCAAAGA TGAGAATTAT TCCGAATGGT ATGTCCAGGT TATAACGAAA GCAGAAATGA TCGAATATTA CGACATTTCT GGTTGTTATG TATTGCGACC GTGGTCTTAC GCTATTTGGG AATTTATTCA GGAATGGTTC GATGAAGAAA TTAAGAAATT AGGTGTTAAA AATTGCTATT TTCCATTATT CGTTTCGCAA AGCGCTTTGG AAAAAGAGAA GACGCACATT AGCGACTTTG CTCCTGAGGT TGCGTGGATA ACGCGAGCAG GACAGTCGGA TCTAGCTGAA GCGATTGCAA TACGTCCTAC TAGCGAAACA GTAATGTATC CCAGCTATGC AAAATGGGTA CAATCTCATC GAGATCTTCC AATTAAGCTC AATCAGTGGT GTAATGTTGT TCGTTGGGAA TTCAAACATC CAACACCATT CTTGAGAACT CGTGAATTTT TGTGGCAAGA AGGTCATACT GCATTCCAAT CCAAAGATGA AGCAGAAGAT GAAGTATTCA AAATACTTGA TTTATATGCT CAAATCTATA TCGATTTACT GGCCATACCA GTTATTAAAG GACGGAAAAC TGAGAAAGAG AACTTTGCTG GCGGAGATTT TACAGCTACT GTTGAGGCAT ATGTGCCTGT CAATGGACGT GGGATACAGG GTGCTACATC GCATCATTTA GGACAAAATT TCTCAAAAAT GTTTAACATA TCGTTTGAAG ACCCAAACGG TGGCGGAAAA ATTTATGCAT GGCAGAATAG TTGGGGTATA TCAACCAGAA CAATTGGTGC TCTTGTGATG ATACACGGTG ATAATTGTGG TCTGGTGCTT CCTCCTAGAG TAGCTACTAT CCAGATGATT ATTGTTCCCG TAGGAATCAA CGCTCAAACA AAGGATGAAC AAAAAACAGC TCTAATTGAA AAAGCAAAAG AAATCAATAA CAAGTTAATG GATGCAAGTA TACGTGCTGA GCTTGATATA AGGGATCATA TATCGCCTGG ATGGAAATTC AATCATTGGG AATTGAAAGG TGTTCCTGTA AGAATCGAAA TTGGTCCCAA AGATTTAGCC AATAACCAGG TAACTTGCGT CATCAGATAT TCTGGCGAGA AAAGAACCAT TCCTATCGAT GGTCTTGCTT CAAAATGCAA AGATATGCTG GAGGAAATTC ACTATTCCAT GTATAATAGG ATATTGGAAG TTCGCGAATC GCACACAAAG ATTGTATTGG AATGGAATGA CTTTCGAAGT TTTCTAGATC AAAAGTTTAT TCTGCTTTCA CCCTTTTGTG GTCGTATTGA GTGCGAAGAT GAAATCAAAA AAGAAAGCAT GAGAGGTGAG GAAAATGATC CTCAAGCTCC CGCAATGGGA GCAAAAACCC TCTGCATTCC GCTTGAACAG CCCGAAATTC CGCTTCCGAC TAACTGTATC AATAAAAATT GTCCAGAAAA ACCACAGTTT TTTGCACTAT TTGGTCGAAG TTATTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT