OnvoA.17194.a
prolyl-trna synthetase (ProRS)-like protein; uncharacterized protein
CENTER ID:
OnvoA.17194.a
ORGANISM:
Onchocerca volvulus
ASSOCIATED DISEASE:
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
NA
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
I
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
OnvoA.17194.a.B1.GE39189
true full-length
info
1
550
OnvoA.17194.a.B1.GE39189
OnvoA.17194.a.B2.GE40762
potential soluble domain
info
58
536
OnvoA.17194.a.B2.GE40762
OnvoA.17194.a.B5.GE40763
XtalPred structured domain #1
info
54
550
OnvoA.17194.a.B5.GE40763
OnvoA.17194.a.MB1.GE39288
true full-length
info
1
550
OnvoA.17194.a.MB1.GE39288
OnvoA.17194.a.MB2.GE39289
potential soluble domain
info
58
536
OnvoA.17194.a.MB2.GE39289
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
OnvoA.17194.a.MB1.PS02533
true full-length
info
1
550
PS02533
OnvoA.17194.a.B5.PW38185
XtalPred structured domain #1
info
54
550
PW38185
Structures
6MN8
DEPOSITED: 10/1/2018
DETERMINATION: XRay
CLONE: OnvoA.17194.a.B5.GE40763
PROTEIN: OnvoA.17194.a.B5.PW38185
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALVNNGDDT LGMKITSQKN AMRKLENNLS KSHISNSTPM KGRGDTKKQT KLGIEISKDE NYSEWYVQVI TKAEMIEYYD ISGCYVLRPW SYAIWEFIQE WFDEEIKKLG VKNCYFPLFV SQSALEKEKT HISDFAPEVA WITRAGQSDL AEAIAIRPTS ETVMYPSYAK WVQSHRDLPI KLNQWCNVVR WEFKHPTPFL RTREFLWQEG HTAFQSKDEA EDEVFKILDL YAQIYIDLLA IPVIKGRKTE KENFAGGDFT ATVEAYVPVN GRGIQGATSH HLGQNFSKMF NISFEDPNGG GKIYAWQNSW GISTRTIGAL VMIHGDNCGL VLPPRVATIQ MIIVPVGINA QTKDEQKTAL IEKAKEINNK LMDASIRAEL DIRDHISPGW KFNHWELKGV PVRIEIGPKD LANNQVTCVI RYSGEKRTIP IDGLASKCKD MLEEIHYSMY NRILEVRESH TKIVLEWNDF RSFLDQKFIL LSPFCGRIEC EDEIKKESMR GEENDPQAPA MGAKTLCIPL EQPEIPLPTN CINKNCPEKP QFFALFGRSY XMSDVESDFD EEAHAKLLKT INSLGNNAKK GPLIRKCPEK VRVKELVDLI HSTRNVDDVK KKLVEKKKKS NKAGKDEPRT LQAPSHRLVR ERIESTIAYS EIRKDLEEWA PIVKKNRLAE QLVFPLSKDP PIQRTASDLV LLFKPRTPLE LELAKLLKTS NNNLRDGEEY TEAELELIRA MSLKEAKAKW AQLKKMRALV GYREAKLKRQ AKIKSKTYHR HMKRQKRKKL IREFEEMMAK DPEAAKEKLE EIDRQRILER ATLKHRNGGK GVQQLARYAN KNKDFKKMYE EQIRLGRELA EKHGREKGSD SDSESENRDA VKLTSAEMLE RAAEVLEKEE QEKRSTKPEL KAKLYEMRKE KRETLRSSAL RATDAKFISN RKYDMNTDVA DESTACNEKV VELEREPEET IRSEEHSNKE QNQQNISENS GQDVEKKANK HSEKDLKNLK KKKSSKKMKN KKENKAAKDL DIDQLFDKAE KELAENALKE CERLKLEDEE NKRNQRHPPS DIRSIVIPQQ KEKKEITAKT EETVTDISLD PRHFLQIETM ALPQVSGDFV ETLEDTSHQT DDQEEIIAKA FEDVDVIGDF EAEKEAVEAA ENPKDIDLTL QGWGSWTGPG ITDRKKDKFI IKMPKKKRKD AGKTGLIISE AVDQSIEKLQ LKSVPFPYTT VEDYEAVIRQ PLGKEWNPQK IHRKLIQPQI VTKAGRIIKP LDKSILQDES EEEKI
NT Sequence
atggcattag tgaataatgg agatgacaca ttgggaatga aaattacttc acaaaaaaat gctatgcgaa agcttgaaaa taatctatct aaatctcata taagtaatag tacacctatg aaaggtcgag gggatacgaa gaaacaaact aaattgggca ttgaaatatc caaagatgag aattattccg aatggtatgt ccaggttata acgaaagcag aaatgatcga atattacgac atttctggtt gttatgtatt gcgaccgtgg tcttacgcta tttgggaatt tattcaggaa tggttcgatg aagaaattaa gaaattaggt gttaaaaatt gctattttcc attattcgtt tcgcaaagcg ctttggaaaa agagaagacg cacattagcg actttgctcc tgaggttgcg tggataacgc gagcaggaca gtcggatcta gctgaagcga ttgcaatacg tcctactagc gaaacagtaa tgtatcccag ctatgcaaaa tgggtacaat ctcatcgaga tcttccaatt aagctcaatc agtggtgtaa tgttgttcgt tgggaattca aacatccaac accattcttg agaactcgtg aatttttgtg gcaagaaggt catactgcat tccaatccaa agatgaagca gaagatgaag tattcaaaat acttgattta tatgctcaaa tctatatcga tttactggcc ataccagtta ttaaaggacg gaaaactgag aaagagaact ttgctggcgg agattttaca gctactgttg aggcatatgt gcctgtcaat ggacgtggga tacagggtgc tacatcgcat catttaggac aaaatttctc aaaaatgttt aacatatcgt ttgaagaccc aaacggtggc ggaaaaattt atgcatggca gaatagttgg ggtatatcaa ccagaacaat tggtgctctt gtgatgatac acggtgataa ttgtggtctg gtgcttcctc ctagagtagc tactatccag atgattattg ttcccgtagg aatcaacgct caaacaaagg atgaacaaaa aacagctcta attgaaaaag caaaagaaat caataacaag ttaatggatg caagtatacg tgctgagctt gatataaggg atcatatatc gcctggatgg aaattcaatc attgggaatt gaaaggtgtt cctgtaagaa tcgaaattgg tcccaaagat ttagccaata accaggtaac ttgcgtcatc agatattctg gcgagaaaag aaccattcct atcgatggtc ttgcttcaaa atgcaaagat atgctggagg aaattcacta ttccatgtat aataggatat tggaagttcg cgaatcgcac acaaagattg tattggaatg gaatgacttt cgaagttttc tagatcaaaa gtttattctg ctttcaccct tttgtggtcg tattgagtgc gaagatgaaa tcaaaaaaga aagcatgaga ggtgaggaaa atgatcctca agctcccgca atgggagcaa aaaccctctg cattccgctt gaacagcccg aaattccgct tccgactaac tgtatcaata aaaattgtcc agaaaaacca cagttttttg cactatttgg tcgaagttat tgaatgtcag atgtggagtc ggattttgat gaggaagccc atgcgaagtt gctgaagact atcaacagct tgggcaataa tgctaagaaa ggaccgttga tcaggaaatg tccggaaaaa gttcgggtta aggaacttgt tgatctgata cattctacaa gaaatgttga tgatgtaaag aagaaattgg tggagaagaa aaagaaatca aacaaagccg gaaaggacga accgagaact ttgcaagctc catctcacag actggttcgg gagaggattg aaagtaccat tgcatacagt gaaattcgaa aggatttaga agaatgggct ccaattgtta aaaagaatcg gcttgctgag caattagtat tcccacttag taaagaccca ccgatccagc gaacagcgtc ggatttagtg cttcttttca agccaaggac accactggaa ctagaattag ccaagttatt gaaaacatca aataataatc ttcgtgatgg cgaggaatat acagaagctg agttggaatt aataagggca atgagtctaa aagaggcaaa agcaaaatgg gctcaattga aaaaaatgcg ggctttggtt ggttaccgag aagctaaact gaaacgacaa gctaaaataa aaagcaaaac atatcatcga catatgaaac gccagaagag aaagaagtta atcagggaat ttgaagaaat gatggccaag gatccagaag ctgccaaaga gaaactggaa gaaattgatc gacagcgcat cctggaacga gctaccttaa agcatcgcaa cggcggaaaa ggtgtccaac agttggcaag atatgcgaat aaaaataagg atttcaaaaa aatgtacgaa gagcaaataa gattgggacg agaactcgcc gaaaagcatg gtcgtgagaa aggttcagat tctgattctg aatcggaaaa tagagatgca gtaaaactaa ctagcgctga aatgcttgag agagcagccg aagtgttaga gaaagaagaa caggaaaaga gatccactaa accagaattg aaagcgaaac tttacgaaat gagaaaagag aagcgggaga ctctcagaag tagtgcattg cgggccacag atgcgaagtt catatctaat cgaaagtatg atatgaatac tgatgtagcc gatgagtcga ctgcttgcaa tgaaaaagtt gtggaattgg aacgggaacc ggaagaaaca ataagaagtg aagagcattc gaacaaagag cagaatcagc aaaatatctc agaaaacagt ggacaagatg ttgaaaaaaa agctaacaaa cactccgaaa aagatctcaa aaatttgaaa aagaagaaaa gttccaagaa aatgaagaac aaaaaagaaa ataaagctgc aaaggacttg gatattgatc agttattcga taaagctgag aaagaacttg ctgaaaatgc tctaaaggag tgtgaacgtc taaaattaga ggacgaagaa aataagcgta atcagagaca tcctccgtct gacattagat ctatagtgat tccccaacaa aaagaaaaaa aagaaatcac tgcaaaaaca gaggaaacag tcacagatat ttctttggat ccaaggcatt ttctacaaat cgaaacaatg gcacttccac aagtctcggg cgactttgtt gaaactttag aagatactag tcaccaaaca gatgatcagg aagaaatcat agccaaggca ttcgaagatg ttgatgttat tggtgatttc gaagctgaaa aggaggccgt ggaagcggca gagaatccga aagatatcga tttaacgctt cagggatggg gttcatggac aggaccagga ataaccgata gaaaaaaaga caagtttata atcaaaatgc cgaaaaagaa gaggaaagat gcagggaaaa caggtttgat tatatcagaa gcagtagatc agtctattga aaaactccaa ctaaaatcgg tgccattccc ttatacaact gttgaggatt atgaagctgt aatacggcaa ccgttaggaa aggaatggaa tccacaaaaa attcatagga agcttattca accgcagatt gtaacaaaag cgggtcgaat aattaaacca ttggataaga gcatattgca agacgaatct gaagaagaaa aaatatga
Details for OnvoA.17194.a.B1.GE39189
HARVESTED ON:
data unavailable
SEQUENCED ON:
5/6/2015
EXPECTED MW:
64kDa
OBSERVED MW:
40kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for OnvoA.17194.a.B2.GE40762
HARVESTED ON:
11/21/2016
SEQUENCED ON:
11/22/2016
EXPECTED MW:
56kDa
OBSERVED MW:
56kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
No Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
98
Details for OnvoA.17194.a.B5.GE40763
HARVESTED ON:
11/21/2016
SEQUENCED ON:
11/22/2016
EXPECTED MW:
58kDa
OBSERVED MW:
58kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for OnvoA.17194.a.MB1.GE39288
HARVESTED ON:
6/25/2015
SEQUENCED ON:
data unavailable
EXPECTED MW:
108kDa
OBSERVED MW:
94kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for OnvoA.17194.a.MB2.GE39289
HARVESTED ON:
data unavailable
SEQUENCED ON:
data unavailable
EXPECTED MW:
100kDa
OBSERVED MW:
46kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
27
PERCENT COVERAGE:
9
Details for OnvoA.17194.a.MB1.PS02533
PURIFICATION DATe:
11/5/2015
CONCENTRATION:
43mg/ml
OBSERVED MW:
95kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
SEC buffer
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
1
VIAL VOLUME:
110µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for OnvoA.17194.a.B5.PW38185
PURIFICATION DATe:
1/30/2017
CONCENTRATION:
17mg/ml
OBSERVED MW:
65kDa
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
BL 21 (DE3) Rosetta
VIAL COUNT (approx.):
2
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100