SSGCID: Target Page

OnvoA.01486.a

Uncharacterized protein (target class Cathepsin B/L)

CENTER ID:	OnvoA.01486.a
ORGANISM:	Onchocerca volvulus
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	NA
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
OnvoA.01486.a.B2.GE44556	mature protein		19	483

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A2K6WCG9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLASSCWVIL LGLHALVAFE KPVVDNHHDA DLYEDLLSLS ITEYNKISKD NYRWIPVEIL DINDIYNTGV HFSIKIAIAL SNCTKVMHTN FDNKHCTAKK ATGLKACIFE IFRPNWRKSA KTKLFTCSNY DSSEELIETS GRASDVSVTV DMKSVESKTN VSQEWLEPKT VMLWNNFLNF VNKFKGSYSS KMEQLNRFKI YMQNMHFAQK LQYEEKGTAI YGETQFSDMM PEEFRKIMLP NLWWQSVESN KKNSNMDFDL EKFNLSLNDL PRSFDWREKG VVTPVKNQGS CGSCWAFSVT GNIESLWAIK KGDLISLSEQ ELIDCDVVDK GCNGGLPINA FREIIRMGGL EPEDQYPYKA KNGTCHLVRT EIAVSIDDAV EIPRNETIMQ AWIAQRGPLS VGIDAELLAY YKSGILHPSR KRCPPSKINH GVLITGYGIE DDLPYWTIKN SWSQLWGEDG YFRLRRGRDV CGVSDLVSSA VIY

NT Sequence

atgttggcca gcagctgttg ggtaatttta ttagggcttc atgccctggt agcttttgag aagcccgtgg ttgataatca tcatgatgca gacttatatg aggatttgtt atcattatca atcacagaat acaacaaaat ctccaaagat aattaccggt ggattccagt ggaaattttg gatattaatg atatttataa tactggagta catttttcga tcaaaattgc aatcgctctt tcgaattgta caaaagttat gcataccaac tttgacaata aacattgcac agcgaagaaa gcaacaggtt tgaaggcttg catatttgaa atatttcgac caaattggag aaaatctgcg aaaactaagc tttttacatg ttccaactat gattcatcag aagaattaat cgaaacgagc ggtcgcgcat cagatgttag tgtgacagtg gacatgaagt ctgtagaatc aaaaactaat gtctcgcaag agtggctaga accaaagacc gtcatgttat ggaataattt cttaaatttc gtcaacaaat tcaaaggatc atattcttcc aaaatggaac agttgaatcg tttcaaaata tatatgcaaa atatgcattt tgctcagaaa ttgcaatatg aagagaaagg aacagcgatt tatggcgaaa cacaattcag cgatatgatg cctgaagaat tcagaaaaat aatgttgccg aatttgtggt ggcaaagcgt agaatccaac aaaaaaaatt caaacatgga tttcgattta gaaaaattta acctttcatt gaatgattta ccaaggagtt ttgattggcg cgaaaaagga gttgtaaccc cagtaaaaaa tcaaggttcc tgtggtagtt gttgggcttt ttctgttact ggcaatattg agagcctctg ggctattaaa aaaggcgact tgatatcact ctcagaacaa gaattgattg attgcgatgt ggttgataaa ggatgcaacg gaggattacc aatcaatgct ttccgagaaa taataagaat gggtggtttg gaaccggaag atcaataccc atataaagcg aaaaatggaa cgtgccattt ggtccgaaca gaaattgctg ttagtatcga tgatgcggtg gaaattccac gtaatgagac aatcatgcaa gcgtggattg ctcaacgtgg gccactttct gttggaattg atgccgaact gctagcatat tataaaagtg gaattctcca tccatcccga aaacgttgtc caccgtcgaa aatcaatcat ggagtactga tcaccggcta tggtattgaa gatgatctac cctattggac gatcaagaat agctggagtc agctgtgggg tgaagatggt tactttcgtt tgaggcgtgg cagagatgtt tgtggtgtta gcgatttggt atcatctgca gtcatctact ga

Details for OnvoA.01486.a.B2.GE44556

HARVESTED ON:	11/2/2021
SEQUENCED ON:	10/28/2021
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHFE KPVVDNHHDA DLYEDLLSLS ITEYNKISKD NYRWIPVEIL DINDIYNTGV HFSIKIAIAL SNCTKVMHTN FDNKHCTAKK ATGLKACIFE IFRPNWRKSA KTKLFTCSNY DSSEELIETS GRASDVSVTV DMKSVESKTN VSQEWLEPKT VMLWNNFLNF VNKFKGSYSS KMEQLNRFKI YMQNMHFAQK LQYEEKGTAI YGETQFSDMM PEEFRKIMLP NLWWQSVESN KKNSNMDFDL EKFNLSLNDL PRSFDWREKG VVTPVKNQGS CGSCWAFSVT GNIESLWAIK KGDLISLSEQ ELIDCDVVDK GCNGGLPINA FREIIRMGGL EPEDQYPYKA KNGTCHLVRT EIAVSIDDAV EIPRNETIMQ AWIAQRGPLS VGIDAELLAY YKSGILHPSR KRCPPSKINH GVLITGYGIE DDLPYWTIKN SWSQLWGEDG YFRLRRGRDV CGVSDLVSSA VIY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattttgaga agcccgtggt tgataatcat catgatgcag acttatatga ggatttgtta tcattatcaa tcacagaata caacaaaatc tccaaagata attaccggtg gattccagtg gaaattttgg atattaatga tatttataat actggagtac atttttcgat caaaattgca atcgctcttt cgaattgtac aaaagttatg cataccaact ttgacaataa acattgcaca gcgaagaaag caacaggttt gaaggcttgc atatttgaaa tatttcgacc aaattggaga aaatctgcga aaactaagct ttttacatgt tccaactatg attcatcaga agaattaatc gaaacgagcg gtcgcgcatc agatgttagt gtgacagtgg acatgaagtc tgtagaatca aaaactaatg tctcgcaaga gtggctagaa ccaaagaccg tcatgttatg gaataatttc ttaaatttcg tcaacaaatt caaaggatca tattcttcca aaatggaaca gttgaatcgt ttcaaaatat atatgcaaaa tatgcatttt gctcagaaat tgcaatatga agagaaagga acagcgattt atggcgaaac acaattcagc gatatgatgc ctgaagaatt cagaaaaata atgttgccga atttgtggtg gcaaagcgta gaatccaaca aaaaaaattc aaacatggat ttcgatttag aaaaatttaa cctttcattg aatgatttac caaggagttt tgattggcgc gaaaaaggag ttgtaacccc agtaaaaaat caaggttcct gtggtagttg ttgggctttt tctgttactg gcaatattga gagcctctgg gctattaaaa aaggcgactt gatatcactc tcagaacaag aattgattga ttgcgatgtg gttgataaag gatgcaacgg aggattacca atcaatgctt tccgagaaat aataagaatg ggtggtttgg aaccggaaga tcaataccca tataaagcga aaaatggaac gtgccatttg gtccgaacag aaattgctgt tagtatcgat gatgcggtgg aaattccacg taatgagaca atcatgcaag cgtggattgc tcaacgtggg ccactttctg ttggaattga tgccgaactg ctagcatatt ataaaagtgg aattctccat ccatcccgaa aacgttgtcc accgtcgaaa atcaatcatg gagtactgat caccggctat ggtattgaag atgatctacc ctattggacg atcaagaata gctggagtca gctgtggggt gaagatggtt actttcgttt gaggcgtggc agagatgttt gtggtgttag cgatttggta tcatctgcag tcatctactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat