OnvoA.00825.a

aspartyl-tRNA synthetase (AspRS)-like protein; uncharacterized protein

CENTER ID: OnvoA.00825.a
ORGANISM: Onchocerca volvulus
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: NA
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
OnvoA.00825.a.B1.GE39188 full length 1 532
OnvoA.00825.a.B2.GE40639 truncation 1 51 532
OnvoA.00825.a.B4.GE40641 truncation 3 61 532
OnvoA.00825.a.B5.GE40642 truncation 4 74 532
OnvoA.00825.a.B6.GE40643 truncation 5 33 532
OnvoA.00825.a.B7.GE40644 truncation 6 9 532
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
OnvoA.00825.a.B2.PW38172 truncation 1 51 532
OnvoA.00825.a.B6.PW38173 truncation 5 33 532
OnvoA.00825.a.B7.PW38174 truncation 6 9 532
OnvoA.00825.a.B1.PW38178 full length 1 532

External Resources

RESOURCE REFERENCE ID
UniProt: A0A044V4G8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTEDGVSMD GAEGEKKLSK KEINKLAKQQ KKMEKKAEHA APDVGENERE MEEDVSEGRY GAYGIIQSAE RKKLDFIDVK DVLVNLEGQE IWIRGRLHTS RIKGKTCFII IRQQMHSIQG MLFVGKEISK QMLKFVGNIS KESIVDVRGT VRKVEQEIIS CTQREVEIHV TQLFVVSAAE PRLPLQIEDA ARIKEESKSA EDSSLSIVNL DTRLDNRVLD LRTQTSHAIF SIQAGVCELF RNTLKKRGFM EIHTPKIISA ASEGGANVSY FKGSAYLAQS PQLYKQMAIA ADFDKVFTIG AVFRAEDSNT HRHLTEFVGL DIEMAFKFHY HEVLETIGAV LIDIFKDLQA KFKHEIETVG KQYPCEPFEF IEPALILKYP DAVKLLRENG VEIGDEDDLS TPVEKFLGRL VKEKYQTDFY ILDRYPLAVR PFYTMPDAND PKYSNSYDMF MRGEEILSGA QRIHDASLLT ERAKHHQIDL EKIRAYIDSF KYGCPPHAGG GIGLERVTML FLGLGNVRLA SLFPRDPKRI TP
NT Sequence
atgacaactg aagatggagt aagtatggat ggagctgaag gtgaaaagaa attgtcaaaa aaagaaataa acaaattggc gaagcaacaa aagaaaatgg aaaagaaggc tgagcatgca gctccagatg taggggaaaa tgagagagaa atggaagaag atgtctcgga aggtcgctac ggtgcatatg gaattataca atctgctgaa cgaaaaaaat tggatttcat tgatgttaaa gatgtcttgg tgaatttaga aggccaggag atttggattc ggggccgttt gcacacatcg aggataaagg ggaaaacgtg cttcattatt atccgtcagc aaatgcattc catacaggga atgttgttcg ttggtaaaga gattagcaaa caaatgttga agtttgttgg aaacatttcg aaagaatcaa ttgttgatgt tcgaggcact gtaagaaaag tggaacaaga aatcatcagc tgtacacaga gagaagtcga aatacatgtt acacagttgt tcgttgttag cgcagcagaa ccacgcttac cactgcagat agaagatgct gctcgaatta aagaagaaag caaaagtgca gaagattcat ctttatctat tgtgaatctc gacacacgtt tagataacag agtactggat cttcgtacgc agacaagtca tgctattttc tctatccaag ctggtgtttg tgaattgttc cgaaacactt taaagaagcg tggtttcatg gaaatacata cgccaaaaat catatctgca gcgagcgaag gaggcgcaaa tgtatcttat tttaaaggct ctgcttacct tgctcagtca ccacagttat ataaacaaat ggcaatcgct gcagattttg acaaagtttt cactattggt gctgtatttc gtgcagaaga cagcaatact catcggcatt taacagaatt tgtcggcctt gatattgaaa tggcgtttaa gttccattac catgaggttc ttgaaacgat tggcgctgtt ctgattgata tattcaaaga tttgcaagcg aaattcaagc atgaaatcga gacagttggc aagcagtatc cttgtgaacc attcgaattc attgagcctg ctcttatact caaatatcca gatgcagtca aactgttacg agagaatggt gtagaaatag gcgatgaaga tgatttaagc actccggtag aaaaatttct gggaaggttg gtgaaagaaa aatatcagac cgatttttac atactcgaca gatatccact agcagttcgt ccattttata cgatgccaga cgcaaacgat ccaaaatact ccaacagtta tgatatgttt atgcgaggtg aagaaatatt atctggcgct cagcgaatcc atgatgcatc tttattaact gaaagagcta aacatcacca aatcgatttg gaaaagatcc gagcttatat tgactcgttc aagtatggct gcccgccaca tgcaggaggt ggcatcggat tagaacgtgt aacgatgctg tttcttggac ttggaaacgt ccgtttggca tctcttttcc cgcgtgaccc taaacgaata acaccataa
Details for OnvoA.00825.a.B1.GE39188
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 61kDa
OBSERVED MW: 61kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MTTEDGVSMD GAEGEKKLSK KEINKLAKQQ KKMEKKAEHA APDVGENERE MEEDVSEGRY GAYGIIQSAE RKKLDFIDVK DVLVNLEGQE IWIRGRLHTS RIKGKTCFII IRQQMHSIQG MLFVGKEISK QMLKFVGNIS KESIVDVRGT VRKVEQEIIS CTQREVEIHV TQLFVVSAAE PRLPLQIEDA ARIKEESKSA EDSSLSIVNL DTRLDNRVLD LRTQTSHAIF SIQAGVCELF RNTLKKRGFM EIHTPKIISA ASEGGANVFE VSYFKGSAYL AQSPQLYKQM AIAADFDKVF TIGAVFRAED SNTHRHLTEF VGLDIEMAFK FHYHEVLETI GAVLIDIFKD LQAKFKHEIE TVGKQYPCEP FEFIEPALIL KYPDAVKLLR ENGVEIGDED DLSTPVEKFL GRLVKEKYQT DFYILDRYPL AVRPFYTMPD ANDPKYSNSY DMFMRGEEIL SGAQRIHDAS LLTERAKHHQ IDLEKIRAYI DSFKYGCPPH AGGGIGLERV TMLFLGLGNV RLASLF
Validated NT Sequence
cgggaaaaga gatgccaaac ggacgtttcc aagtccaaga aacagcatcg ttacacgttc taatccgatg ccacctcctg catgtggcgg gcagccatac ttgaacgagt caatataagc tcggatcttt tccaaatcga tttggtgatg tttagctctt tcagttaata aagatgcatc atggattcgc tgagcgccag ataatatttc ttcacctcgc ataaacatat cataactgtt ggagtatttt ggatcgtttg cgtctggcat cgtataaaat ggacgaactg ctagtggata tctgtcgagt atgtaaaaat cggtctgata tttttctttc accaaccttc ccagaaattt ttctaccgga gtgcttaaat catcttcatc gcctatttct acaccattct ctcgtaacag tttgactgca tctggatatt tgagtataag agcaggctca atgaattcga atggttcaca aggatactgc ttgccaactg tctcgatttc atgcttgaat ttcgcttgca aatctttgaa tatatcaatc agaacagcgc caatcgtttc aagaacctca tggtaatgga acttaaacgc catttcaata tcaaggccga caaattctgt taaatgccga tgagtattgc tgtcttctgc acgaaataca gcaccaatag tgaaaacttt gtcaaaatct gcagcgattg ccatttgttt atataactgt ggtgactgag caaggtaagc agagccttta aaataagata cctcaaagac atttgcgcct ccttcgctcg ctgcagatat gatttttggc gtatgtattt ccatgaaacc acgcttcttt aaagtgtttc ggaacaattc acaaacacca gcttggatag agaaaatagc atgacttgtc tgcgtacgaa gatccagtac tctgttatct aaacgtgtgt cgagattcac aatagataaa gatgaatctt ctgcactttt gctttcttct ttaattcgag cagcatcttc tatctgcagt ggtaagcgtg gttctgctgc gctaacaacg aacaactgtg taacatgtat ttcgacttct ctctgtgtac agctgatgat ttcttgttcc acttttctta cagtgcctcg aacatcaaca attgattctt tcgaaatgtt tccaacaaac ttcaacattt gtttgctaat ctctttacca acgaacaaca ttccctgtat ggaatgcatt tgctgacgga taataatgaa gcacgttttc ccctttatcc tcgatgtgtg caaacggccc cgaatccaaa tctcctggcc ttctaaattc accaagacat ctttaacatc aatgaaatcc aatttttttc gttcagcaga ttgtataatt ccatatgcac cgtagcgacc ttccgagaca tcttcttcca tttctctctc attttcccct acatctggag ctgcatgctc agccttcttt tccattttct tttgttgctt cgccaatttg tttatttctt tttttgacaa tttcttttca ccttcagctc catccatact tactccatct tcagttgtca tatggtggtg gtggnnnt
Expected Protein Sequence
MAHHHHHHMT TEDGVSMDGA EGEKKLSKKE INKLAKQQKK MEKKAEHAAP DVGENEREME EDVSEGRYGA YGIIQSAERK KLDFIDVKDV LVNLEGQEIW IRGRLHTSRI KGKTCFIIIR QQMHSIQGML FVGKEISKQM LKFVGNISKE SIVDVRGTVR KVEQEIISCT QREVEIHVTQ LFVVSAAEPR LPLQIEDAAR IKEESKSAED SSLSIVNLDT RLDNRVLDLR TQTSHAIFSI QAGVCELFRN TLKKRGFMEI HTPKIISAAS EGGANVSYFK GSAYLAQSPQ LYKQMAIAAD FDKVFTIGAV FRAEDSNTHR HLTEFVGLDI EMAFKFHYHE VLETIGAVLI DIFKDLQAKF KHEIETVGKQ YPCEPFEFIE PALILKYPDA VKLLRENGVE IGDEDDLSTP VEKFLGRLVK EKYQTDFYIL DRYPLAVRPF YTMPDANDPK YSNSYDMFMR GEEILSGAQR IHDASLLTER AKHHQIDLEK IRAYIDSFKY GCPPHAGGGI GLERVTMLFL GLGNVRLASL FPRDPKRITP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa ctgaagatgg agtaagtatg gatggagctg aaggtgaaaa gaaattgtca aaaaaagaaa taaacaaatt ggcgaagcaa caaaagaaaa tggaaaagaa ggctgagcat gcagctccag atgtagggga aaatgagaga gaaatggaag aagatgtctc ggaaggtcgc tacggtgcat atggaattat acaatctgct gaacgaaaaa aattggattt cattgatgtt aaagatgtct tggtgaattt agaaggccag gagatttgga ttcggggccg tttgcacaca tcgaggataa aggggaaaac gtgcttcatt attatccgtc agcaaatgca ttccatacag ggaatgttgt tcgttggtaa agagattagc aaacaaatgt tgaagtttgt tggaaacatt tcgaaagaat caattgttga tgttcgaggc actgtaagaa aagtggaaca agaaatcatc agctgtacac agagagaagt cgaaatacat gttacacagt tgttcgttgt tagcgcagca gaaccacgct taccactgca gatagaagat gctgctcgaa ttaaagaaga aagcaaaagt gcagaagatt catctttatc tattgtgaat ctcgacacac gtttagataa cagagtactg gatcttcgta cgcagacaag tcatgctatt ttctctatcc aagctggtgt ttgtgaattg ttccgaaaca ctttaaagaa gcgtggtttc atggaaatac atacgccaaa aatcatatct gcagcgagcg aaggaggcgc aaatgtatct tattttaaag gctctgctta ccttgctcag tcaccacagt tatataaaca aatggcaatc gctgcagatt ttgacaaagt tttcactatt ggtgctgtat ttcgtgcaga agacagcaat actcatcggc atttaacaga atttgtcggc cttgatattg aaatggcgtt taagttccat taccatgagg ttcttgaaac gattggcgct gttctgattg atatattcaa agatttgcaa gcgaaattca agcatgaaat cgagacagtt ggcaagcagt atccttgtga accattcgaa ttcattgagc ctgctcttat actcaaatat ccagatgcag tcaaactgtt acgagagaat ggtgtagaaa taggcgatga agatgattta agcactccgg tagaaaaatt tctgggaagg ttggtgaaag aaaaatatca gaccgatttt tacatactcg acagatatcc actagcagtt cgtccatttt atacgatgcc agacgcaaac gatccaaaat actccaacag ttatgatatg tttatgcgag gtgaagaaat attatctggc gctcagcgaa tccatgatgc atctttatta actgaaagag ctaaacatca ccaaatcgat ttggaaaaga tccgagctta tattgactcg ttcaagtatg gctgcccgcc acatgcagga ggtggcatcg gattagaacg tgtaacgatg ctgtttcttg gacttggaaa cgtccgtttg gcatctcttt tcccgcgtga ccctaaacga ataacaccat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for OnvoA.00825.a.B2.GE40639
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 56kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHME EDVSEGRYGA YGIIQSAERK KLDFIDVKDV LVNLEGQEIW IRGRLHTSRI KGKTCFIIIR QQMHSIQGML FVGKEISKQM LKFVGNISKE SIVDVRGTVR KVEQEIISCT QREVEIHVTQ LFVVSAAEPR LPLQIEDAAR IKEESKSAED SSLSIVNLDT RLDNRVLDLR TQTSHAIFSI QAGVCELFRN TLKKRGFMEI HTPKIISAAS EGGANVSYFK GSAYLAQSPQ LYKQMAIAAD FDKVFTIGAV FRAEDSNTHR HLTEFVGLDI EMAFKFHYHE VLETIGAVLI DIFKDLQAKF KHEIETVGKQ YPCEPFEFIE PALILKYPDA VKLLRENGVE IGDEDDLSTP VEKFLGRLVK EKYQTDFYIL DRYPLAVRPF YTMPDANDPK YSNSYDMFMR GEEILSGAQR IHDASLLTER AKHHQIDLEK IRAYIDSFKY GCPPHAGGGI GLERVTMLFL GLGNVRLASL FPRDPKRITP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag aagatgtctc ggaaggtcgc tacggtgcat atggaattat acaatctgct gaacgaaaaa aattggattt cattgatgtt aaagatgtct tggtgaattt agaaggccag gagatttgga ttcggggccg tttgcacaca tcgaggataa aggggaaaac gtgcttcatt attatccgtc agcaaatgca ttccatacag ggaatgttgt tcgttggtaa agagattagc aaacaaatgt tgaagtttgt tggaaacatt tcgaaagaat caattgttga tgttcgaggc actgtaagaa aagtggaaca agaaatcatc agctgtacac agagagaagt cgaaatacat gttacacagt tgttcgttgt tagcgcagca gaaccacgct taccactgca gatagaagat gctgctcgaa ttaaagaaga aagcaaaagt gcagaagatt catctttatc tattgtgaat ctcgacacac gtttagataa cagagtactg gatcttcgta cgcagacaag tcatgctatt ttctctatcc aagctggtgt ttgtgaattg ttccgaaaca ctttaaagaa gcgtggtttc atggaaatac atacgccaaa aatcatatct gcagcgagcg aaggaggcgc aaatgtatct tattttaaag gctctgctta ccttgctcag tcaccacagt tatataaaca aatggcaatc gctgcagatt ttgacaaagt tttcactatt ggtgctgtat ttcgtgcaga agacagcaat actcatcggc atttaacaga atttgtcggc cttgatattg aaatggcgtt taagttccat taccatgagg ttcttgaaac gattggcgct gttctgattg atatattcaa agatttgcaa gcgaaattca agcatgaaat cgagacagtt ggcaagcagt atccttgtga accattcgaa ttcattgagc ctgctcttat actcaaatat ccagatgcag tcaaactgtt acgagagaat ggtgtagaaa taggcgatga agatgattta agcactccgg tagaaaaatt tctgggaagg ttggtgaaag aaaaatatca gaccgatttt tacatactcg acagatatcc actagcagtt cgtccatttt atacgatgcc agacgcaaac gatccaaaat actccaacag ttatgatatg tttatgcgag gtgaagaaat attatctggc gctcagcgaa tccatgatgc atctttatta actgaaagag ctaaacatca ccaaatcgat ttggaaaaga tccgagctta tattgactcg ttcaagtatg gctgcccgcc acatgcagga ggtggcatcg gattagaacg tgtaacgatg ctgtttcttg gacttggaaa cgtccgtttg gcatctcttt tcccgcgtga ccctaaacga ataacaccat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for OnvoA.00825.a.B4.GE40641
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 55kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGA YGIIQSAERK KLDFIDVKDV LVNLEGQEIW IRGRLHTSRI KGKTCFIIIR QQMHSIQGML FVGKEISKQM LKFVGNISKE SIVDVRGTVR KVEQEIISCT QREVEIHVTQ LFVVSAAEPR LPLQIEDAAR IKEESKSAED SSLSIVNLDT RLDNRVLDLR TQTSHAIFSI QAGVCELFRN TLKKRGFMEI HTPKIISAAS EGGANVSYFK GSAYLAQSPQ LYKQMAIAAD FDKVFTIGAV FRAEDSNTHR HLTEFVGLDI EMAFKFHYHE VLETIGAVLI DIFKDLQAKF KHEIETVGKQ YPCEPFEFIE PALILKYPDA VKLLRENGVE IGDEDDLSTP VEKFLGRLVK EKYQTDFYIL DRYPLAVRPF YTMPDANDPK YSNSYDMFMR GEEILSGAQR IHDASLLTER AKHHQIDLEK IRAYIDSFKY GCPPHAGGGI GLERVTMLFL GLGNVRLASL FPRDPKRITP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtgcat atggaattat acaatctgct gaacgaaaaa aattggattt cattgatgtt aaagatgtct tggtgaattt agaaggccag gagatttgga ttcggggccg tttgcacaca tcgaggataa aggggaaaac gtgcttcatt attatccgtc agcaaatgca ttccatacag ggaatgttgt tcgttggtaa agagattagc aaacaaatgt tgaagtttgt tggaaacatt tcgaaagaat caattgttga tgttcgaggc actgtaagaa aagtggaaca agaaatcatc agctgtacac agagagaagt cgaaatacat gttacacagt tgttcgttgt tagcgcagca gaaccacgct taccactgca gatagaagat gctgctcgaa ttaaagaaga aagcaaaagt gcagaagatt catctttatc tattgtgaat ctcgacacac gtttagataa cagagtactg gatcttcgta cgcagacaag tcatgctatt ttctctatcc aagctggtgt ttgtgaattg ttccgaaaca ctttaaagaa gcgtggtttc atggaaatac atacgccaaa aatcatatct gcagcgagcg aaggaggcgc aaatgtatct tattttaaag gctctgctta ccttgctcag tcaccacagt tatataaaca aatggcaatc gctgcagatt ttgacaaagt tttcactatt ggtgctgtat ttcgtgcaga agacagcaat actcatcggc atttaacaga atttgtcggc cttgatattg aaatggcgtt taagttccat taccatgagg ttcttgaaac gattggcgct gttctgattg atatattcaa agatttgcaa gcgaaattca agcatgaaat cgagacagtt ggcaagcagt atccttgtga accattcgaa ttcattgagc ctgctcttat actcaaatat ccagatgcag tcaaactgtt acgagagaat ggtgtagaaa taggcgatga agatgattta agcactccgg tagaaaaatt tctgggaagg ttggtgaaag aaaaatatca gaccgatttt tacatactcg acagatatcc actagcagtt cgtccatttt atacgatgcc agacgcaaac gatccaaaat actccaacag ttatgatatg tttatgcgag gtgaagaaat attatctggc gctcagcgaa tccatgatgc atctttatta actgaaagag ctaaacatca ccaaatcgat ttggaaaaga tccgagctta tattgactcg ttcaagtatg gctgcccgcc acatgcagga ggtggcatcg gattagaacg tgtaacgatg ctgtttcttg gacttggaaa cgtccgtttg gcatctcttt tcccgcgtga ccctaaacga ataacaccat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for OnvoA.00825.a.B5.GE40642
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 53kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLD FIDVKDVLVN LEGQEIWIRG RLHTSRIKGK TCFIIIRQQM HSIQGMLFVG KEISKQMLKF VGNISKESIV DVRGTVRKVE QEIISCTQRE VEIHVTQLFV VSAAEPRLPL QIEDAARIKE ESKSAEDSSL SIVNLDTRLD NRVLDLRTQT SHAIFSIQAG VCELFRNTLK KRGFMEIHTP KIISAASEGG ANVSYFKGSA YLAQSPQLYK QMAIAADFDK VFTIGAVFRA EDSNTHRHLT EFVGLDIEMA FKFHYHEVLE TIGAVLIDIF KDLQAKFKHE IETVGKQYPC EPFEFIEPAL ILKYPDAVKL LRENGVEIGD EDDLSTPVEK FLGRLVKEKY QTDFYILDRY PLAVRPFYTM PDANDPKYSN SYDMFMRGEE ILSGAQRIHD ASLLTERAKH HQIDLEKIRA YIDSFKYGCP PHAGGGIGLE RVTMLFLGLG NVRLASLFPR DPKRITP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggatt tcattgatgt taaagatgtc ttggtgaatt tagaaggcca ggagatttgg attcggggcc gtttgcacac atcgaggata aaggggaaaa cgtgcttcat tattatccgt cagcaaatgc attccataca gggaatgttg ttcgttggta aagagattag caaacaaatg ttgaagtttg ttggaaacat ttcgaaagaa tcaattgttg atgttcgagg cactgtaaga aaagtggaac aagaaatcat cagctgtaca cagagagaag tcgaaataca tgttacacag ttgttcgttg ttagcgcagc agaaccacgc ttaccactgc agatagaaga tgctgctcga attaaagaag aaagcaaaag tgcagaagat tcatctttat ctattgtgaa tctcgacaca cgtttagata acagagtact ggatcttcgt acgcagacaa gtcatgctat tttctctatc caagctggtg tttgtgaatt gttccgaaac actttaaaga agcgtggttt catggaaata catacgccaa aaatcatatc tgcagcgagc gaaggaggcg caaatgtatc ttattttaaa ggctctgctt accttgctca gtcaccacag ttatataaac aaatggcaat cgctgcagat tttgacaaag ttttcactat tggtgctgta tttcgtgcag aagacagcaa tactcatcgg catttaacag aatttgtcgg ccttgatatt gaaatggcgt ttaagttcca ttaccatgag gttcttgaaa cgattggcgc tgttctgatt gatatattca aagatttgca agcgaaattc aagcatgaaa tcgagacagt tggcaagcag tatccttgtg aaccattcga attcattgag cctgctctta tactcaaata tccagatgca gtcaaactgt tacgagagaa tggtgtagaa ataggcgatg aagatgattt aagcactccg gtagaaaaat ttctgggaag gttggtgaaa gaaaaatatc agaccgattt ttacatactc gacagatatc cactagcagt tcgtccattt tatacgatgc cagacgcaaa cgatccaaaa tactccaaca gttatgatat gtttatgcga ggtgaagaaa tattatctgg cgctcagcga atccatgatg catctttatt aactgaaaga gctaaacatc accaaatcga tttggaaaag atccgagctt atattgactc gttcaagtat ggctgcccgc cacatgcagg aggtggcatc ggattagaac gtgtaacgat gctgtttctt ggacttggaa acgtccgttt ggcatctctt ttcccgcgtg accctaaacg aataacacca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for OnvoA.00825.a.B6.GE40643
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 58kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHME KKAEHAAPDV GENEREMEED VSEGRYGAYG IIQSAERKKL DFIDVKDVLV NLEGQEIWIR GRLHTSRIKG KTCFIIIRQQ MHSIQGMLFV GKEISKQMLK FVGNISKESI VDVRGTVRKV EQEIISCTQR EVEIHVTQLF VVSAAEPRLP LQIEDAARIK EESKSAEDSS LSIVNLDTRL DNRVLDLRTQ TSHAIFSIQA GVCELFRNTL KKRGFMEIHT PKIISAASEG GANVSYFKGS AYLAQSPQLY KQMAIAADFD KVFTIGAVFR AEDSNTHRHL TEFVGLDIEM AFKFHYHEVL ETIGAVLIDI FKDLQAKFKH EIETVGKQYP CEPFEFIEPA LILKYPDAVK LLRENGVEIG DEDDLSTPVE KFLGRLVKEK YQTDFYILDR YPLAVRPFYT MPDANDPKYS NSYDMFMRGE EILSGAQRIH DASLLTERAK HHQIDLEKIR AYIDSFKYGC PPHAGGGIGL ERVTMLFLGL GNVRLASLFP RDPKRITP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa agaaggctga gcatgcagct ccagatgtag gggaaaatga gagagaaatg gaagaagatg tctcggaagg tcgctacggt gcatatggaa ttatacaatc tgctgaacga aaaaaattgg atttcattga tgttaaagat gtcttggtga atttagaagg ccaggagatt tggattcggg gccgtttgca cacatcgagg ataaagggga aaacgtgctt cattattatc cgtcagcaaa tgcattccat acagggaatg ttgttcgttg gtaaagagat tagcaaacaa atgttgaagt ttgttggaaa catttcgaaa gaatcaattg ttgatgttcg aggcactgta agaaaagtgg aacaagaaat catcagctgt acacagagag aagtcgaaat acatgttaca cagttgttcg ttgttagcgc agcagaacca cgcttaccac tgcagataga agatgctgct cgaattaaag aagaaagcaa aagtgcagaa gattcatctt tatctattgt gaatctcgac acacgtttag ataacagagt actggatctt cgtacgcaga caagtcatgc tattttctct atccaagctg gtgtttgtga attgttccga aacactttaa agaagcgtgg tttcatggaa atacatacgc caaaaatcat atctgcagcg agcgaaggag gcgcaaatgt atcttatttt aaaggctctg cttaccttgc tcagtcacca cagttatata aacaaatggc aatcgctgca gattttgaca aagttttcac tattggtgct gtatttcgtg cagaagacag caatactcat cggcatttaa cagaatttgt cggccttgat attgaaatgg cgtttaagtt ccattaccat gaggttcttg aaacgattgg cgctgttctg attgatatat tcaaagattt gcaagcgaaa ttcaagcatg aaatcgagac agttggcaag cagtatcctt gtgaaccatt cgaattcatt gagcctgctc ttatactcaa atatccagat gcagtcaaac tgttacgaga gaatggtgta gaaataggcg atgaagatga tttaagcact ccggtagaaa aatttctggg aaggttggtg aaagaaaaat atcagaccga tttttacata ctcgacagat atccactagc agttcgtcca ttttatacga tgccagacgc aaacgatcca aaatactcca acagttatga tatgtttatg cgaggtgaag aaatattatc tggcgctcag cgaatccatg atgcatcttt attaactgaa agagctaaac atcaccaaat cgatttggaa aagatccgag cttatattga ctcgttcaag tatggctgcc cgccacatgc aggaggtggc atcggattag aacgtgtaac gatgctgttt cttggacttg gaaacgtccg tttggcatct cttttcccgc gtgaccctaa acgaataaca ccatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for OnvoA.00825.a.B7.GE40644
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 61kDa
OBSERVED MW: 63kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMD GAEGEKKLSK KEINKLAKQQ KKMEKKAEHA APDVGENERE MEEDVSEGRY GAYGIIQSAE RKKLDFIDVK DVLVNLEGQE IWIRGRLHTS RIKGKTCFII IRQQMHSIQG MLFVGKEISK QMLKFVGNIS KESIVDVRGT VRKVEQEIIS CTQREVEIHV TQLFVVSAAE PRLPLQIEDA ARIKEESKSA EDSSLSIVNL DTRLDNRVLD LRTQTSHAIF SIQAGVCELF RNTLKKRGFM EIHTPKXISA ASEGGANVFE VSYFKGSAYL AQSPQLYKQM AIAADFDKVF TIGAVFRAED SNTHRHLTEF VGLDIEMAFK FHYHEVLETI GAVLIDIFKD LQAKFKHEIE TVGKQYPCEP FEFIEPALIL KYPDAVKLLR ENGVEIGDED DLSTPVEKFL GRLVKEKYQT DFYILDRYPL AVRPFYTMPD ANDPKYSNSY DMFMRGEEIL SGAQRIHDAS LLTERAKHHQ IDLEKIRAYI DSFKYGCPPH AGGGIGLERV TMLFLGLGNV RLASLFP
Validated NT Sequence
gcgggaaaag agatgccaaa cggacgtttc caagtccaag aaacagcatc gttacacgtt ctaatccgat gccacctcct gcatgtggcg ggcagccata cttgaacgag tcaatataag ctcggatctt ttccaaatcg atttggtgat gtttagctct ttcagttaat aaagatgcat catggattcg ctgagcgcca gataatattt cttcacctcg cataaacata tcataactgt tggagtattt tggatcgttt gcgtctggca tcgtataaaa tggacgaact gctagtggat atctgtcgag tatgtaaaaa tcggtctgat atttttcttt caccaacctt cccagaaatt tttctaccgg agtgcttaaa tcatcttcat cgcctatttc tacaccattc tctcgtaaca gtttgactgc atctggatat ttgagtataa gagcaggctc aatgaattcg aatggttcac aaggatactg cttgccaact gtctcgattt catgcttgaa tttcgcttgc aaatctttga atatatcaat cagaacagcg ccaatcgttt caagaacctc atggtaatgg aacttaaacg ccatttcaat atcaaggccg acaaattctg ttaaatgccg atgagtattg ctgtcttctg cacgaaatac agcaccaata gtgaaaactt tgtcaaaatc tgcagcgatt gccatttgtt tatataactg tggtgactga gcaaggtaag cagagccttt aaaataagat acctcaaaga catttgcgcc tccttcgctc gctgcagata tganttttgg cgtatgtatt tccatgaaac cacgcttctt taaagtgttt cggaacaatt cacaaacacc agcttggata gagaaaatag catgacttgt ctgcgtacga agatccagta ctctgttatc taaacgtgtg tcgagattca caatagataa agatgaatct tctgcacttt tgctttcttc tttaattcga gcagcatctt ctatctgcag tggtaagcgt ggttctgctg cgctaacaac gaacaactgt gtaacatgta tttcgacttc tctctgtgta cagctgatga tttcttgttc cacttttctt acagtgcctc gaacatcaac aattgattct ttcgaaatgt ttccaacaaa cttcaacatt tgtttgctaa tctctttacc aacgaacaac attccctgta tggaatgcat ttgctgacgg ataataatga agcacgtttt cccctttatc ctcgatgtgt gcaaacggcc ccgaatccaa atctcctggc cttctaaatt caccaagaca tctttaacat caatgaaatc caattttttt cgttcagcag attgtataat tccatatgca ccgtagcgac cttccgagac atcttcttcc atttctctct cattttcccc tacatctgga gctgcatgct cagccttctt ttccattttc ttttgttgct tcgccaattt gtttatttct ttttttgaca atttcttttc accttcagct ccatccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMD GAEGEKKLSK KEINKLAKQQ KKMEKKAEHA APDVGENERE MEEDVSEGRY GAYGIIQSAE RKKLDFIDVK DVLVNLEGQE IWIRGRLHTS RIKGKTCFII IRQQMHSIQG MLFVGKEISK QMLKFVGNIS KESIVDVRGT VRKVEQEIIS CTQREVEIHV TQLFVVSAAE PRLPLQIEDA ARIKEESKSA EDSSLSIVNL DTRLDNRVLD LRTQTSHAIF SIQAGVCELF RNTLKKRGFM EIHTPKIISA ASEGGANVSY FKGSAYLAQS PQLYKQMAIA ADFDKVFTIG AVFRAEDSNT HRHLTEFVGL DIEMAFKFHY HEVLETIGAV LIDIFKDLQA KFKHEIETVG KQYPCEPFEF IEPALILKYP DAVKLLRENG VEIGDEDDLS TPVEKFLGRL VKEKYQTDFY ILDRYPLAVR PFYTMPDAND PKYSNSYDMF MRGEEILSGA QRIHDASLLT ERAKHHQIDL EKIRAYIDSF KYGCPPHAGG GIGLERVTML FLGLGNVRLA SLFPRDPKRI TP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatg gagctgaagg tgaaaagaaa ttgtcaaaaa aagaaataaa caaattggcg aagcaacaaa agaaaatgga aaagaaggct gagcatgcag ctccagatgt aggggaaaat gagagagaaa tggaagaaga tgtctcggaa ggtcgctacg gtgcatatgg aattatacaa tctgctgaac gaaaaaaatt ggatttcatt gatgttaaag atgtcttggt gaatttagaa ggccaggaga tttggattcg gggccgtttg cacacatcga ggataaaggg gaaaacgtgc ttcattatta tccgtcagca aatgcattcc atacagggaa tgttgttcgt tggtaaagag attagcaaac aaatgttgaa gtttgttgga aacatttcga aagaatcaat tgttgatgtt cgaggcactg taagaaaagt ggaacaagaa atcatcagct gtacacagag agaagtcgaa atacatgtta cacagttgtt cgttgttagc gcagcagaac cacgcttacc actgcagata gaagatgctg ctcgaattaa agaagaaagc aaaagtgcag aagattcatc tttatctatt gtgaatctcg acacacgttt agataacaga gtactggatc ttcgtacgca gacaagtcat gctattttct ctatccaagc tggtgtttgt gaattgttcc gaaacacttt aaagaagcgt ggtttcatgg aaatacatac gccaaaaatc atatctgcag cgagcgaagg aggcgcaaat gtatcttatt ttaaaggctc tgcttacctt gctcagtcac cacagttata taaacaaatg gcaatcgctg cagattttga caaagttttc actattggtg ctgtatttcg tgcagaagac agcaatactc atcggcattt aacagaattt gtcggccttg atattgaaat ggcgtttaag ttccattacc atgaggttct tgaaacgatt ggcgctgttc tgattgatat attcaaagat ttgcaagcga aattcaagca tgaaatcgag acagttggca agcagtatcc ttgtgaacca ttcgaattca ttgagcctgc tcttatactc aaatatccag atgcagtcaa actgttacga gagaatggtg tagaaatagg cgatgaagat gatttaagca ctccggtaga aaaatttctg ggaaggttgg tgaaagaaaa atatcagacc gatttttaca tactcgacag atatccacta gcagttcgtc cattttatac gatgccagac gcaaacgatc caaaatactc caacagttat gatatgttta tgcgaggtga agaaatatta tctggcgctc agcgaatcca tgatgcatct ttattaactg aaagagctaa acatcaccaa atcgatttgg aaaagatccg agcttatatt gactcgttca agtatggctg cccgccacat gcaggaggtg gcatcggatt agaacgtgta acgatgctgt ttcttggact tggaaacgtc cgtttggcat ctcttttccc gcgtgaccct aaacgaataa caccatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for OnvoA.00825.a.B2.PW38172
PURIFICATION DATe: 1/23/2017
CONCENTRATION: 31mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHME EDVSEGRYGA YGIIQSAERK KLDFIDVKDV LVNLEGQEIW IRGRLHTSRI KGKTCFIIIR QQMHSIQGML FVGKEISKQM LKFVGNISKE SIVDVRGTVR KVEQEIISCT QREVEIHVTQ LFVVSAAEPR LPLQIEDAAR IKEESKSAED SSLSIVNLDT RLDNRVLDLR TQTSHAIFSI QAGVCELFRN TLKKRGFMEI HTPKIISAAS EGGANVSYFK GSAYLAQSPQ LYKQMAIAAD FDKVFTIGAV FRAEDSNTHR HLTEFVGLDI EMAFKFHYHE VLETIGAVLI DIFKDLQAKF KHEIETVGKQ YPCEPFEFIE PALILKYPDA VKLLRENGVE IGDEDDLSTP VEKFLGRLVK EKYQTDFYIL DRYPLAVRPF YTMPDANDPK YSNSYDMFMR GEEILSGAQR IHDASLLTER AKHHQIDLEK IRAYIDSFKY GCPPHAGGGI GLERVTMLFL GLGNVRLASL FPRDPKRITP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAG AAGATGTCTC GGAAGGTCGC TACGGTGCAT ATGGAATTAT ACAATCTGCT GAACGAAAAA AATTGGATTT CATTGATGTT AAAGATGTCT TGGTGAATTT AGAAGGCCAG GAGATTTGGA TTCGGGGCCG TTTGCACACA TCGAGGATAA AGGGGAAAAC GTGCTTCATT ATTATCCGTC AGCAAATGCA TTCCATACAG GGAATGTTGT TCGTTGGTAA AGAGATTAGC AAACAAATGT TGAAGTTTGT TGGAAACATT TCGAAAGAAT CAATTGTTGA TGTTCGAGGC ACTGTAAGAA AAGTGGAACA AGAAATCATC AGCTGTACAC AGAGAGAAGT CGAAATACAT GTTACACAGT TGTTCGTTGT TAGCGCAGCA GAACCACGCT TACCACTGCA GATAGAAGAT GCTGCTCGAA TTAAAGAAGA AAGCAAAAGT GCAGAAGATT CATCTTTATC TATTGTGAAT CTCGACACAC GTTTAGATAA CAGAGTACTG GATCTTCGTA CGCAGACAAG TCATGCTATT TTCTCTATCC AAGCTGGTGT TTGTGAATTG TTCCGAAACA CTTTAAAGAA GCGTGGTTTC ATGGAAATAC ATACGCCAAA AATCATATCT GCAGCGAGCG AAGGAGGCGC AAATGTATCT TATTTTAAAG GCTCTGCTTA CCTTGCTCAG TCACCACAGT TATATAAACA AATGGCAATC GCTGCAGATT TTGACAAAGT TTTCACTATT GGTGCTGTAT TTCGTGCAGA AGACAGCAAT ACTCATCGGC ATTTAACAGA ATTTGTCGGC CTTGATATTG AAATGGCGTT TAAGTTCCAT TACCATGAGG TTCTTGAAAC GATTGGCGCT GTTCTGATTG ATATATTCAA AGATTTGCAA GCGAAATTCA AGCATGAAAT CGAGACAGTT GGCAAGCAGT ATCCTTGTGA ACCATTCGAA TTCATTGAGC CTGCTCTTAT ACTCAAATAT CCAGATGCAG TCAAACTGTT ACGAGAGAAT GGTGTAGAAA TAGGCGATGA AGATGATTTA AGCACTCCGG TAGAAAAATT TCTGGGAAGG TTGGTGAAAG AAAAATATCA GACCGATTTT TACATACTCG ACAGATATCC ACTAGCAGTT CGTCCATTTT ATACGATGCC AGACGCAAAC GATCCAAAAT ACTCCAACAG TTATGATATG TTTATGCGAG GTGAAGAAAT ATTATCTGGC GCTCAGCGAA TCCATGATGC ATCTTTATTA ACTGAAAGAG CTAAACATCA CCAAATCGAT TTGGAAAAGA TCCGAGCTTA TATTGACTCG TTCAAGTATG GCTGCCCGCC ACATGCAGGA GGTGGCATCG GATTAGAACG TGTAACGATG CTGTTTCTTG GACTTGGAAA CGTCCGTTTG GCATCTCTTT TCCCGCGTGA CCCTAAACGA ATAACACCAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT
Details for OnvoA.00825.a.B6.PW38173
PURIFICATION DATe: 1/23/2017
CONCENTRATION: 26.8mg/ml
OBSERVED MW: 60kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHME KKAEHAAPDV GENEREMEED VSEGRYGAYG IIQSAERKKL DFIDVKDVLV NLEGQEIWIR GRLHTSRIKG KTCFIIIRQQ MHSIQGMLFV GKEISKQMLK FVGNISKESI VDVRGTVRKV EQEIISCTQR EVEIHVTQLF VVSAAEPRLP LQIEDAARIK EESKSAEDSS LSIVNLDTRL DNRVLDLRTQ TSHAIFSIQA GVCELFRNTL KKRGFMEIHT PKIISAASEG GANVSYFKGS AYLAQSPQLY KQMAIAADFD KVFTIGAVFR AEDSNTHRHL TEFVGLDIEM AFKFHYHEVL ETIGAVLIDI FKDLQAKFKH EIETVGKQYP CEPFEFIEPA LILKYPDAVK LLRENGVEIG DEDDLSTPVE KFLGRLVKEK YQTDFYILDR YPLAVRPFYT MPDANDPKYS NSYDMFMRGE EILSGAQRIH DASLLTERAK HHQIDLEKIR AYIDSFKYGC PPHAGGGIGL ERVTMLFLGL GNVRLASLFP RDPKRITP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA AGAAGGCTGA GCATGCAGCT CCAGATGTAG GGGAAAATGA GAGAGAAATG GAAGAAGATG TCTCGGAAGG TCGCTACGGT GCATATGGAA TTATACAATC TGCTGAACGA AAAAAATTGG ATTTCATTGA TGTTAAAGAT GTCTTGGTGA ATTTAGAAGG CCAGGAGATT TGGATTCGGG GCCGTTTGCA CACATCGAGG ATAAAGGGGA AAACGTGCTT CATTATTATC CGTCAGCAAA TGCATTCCAT ACAGGGAATG TTGTTCGTTG GTAAAGAGAT TAGCAAACAA ATGTTGAAGT TTGTTGGAAA CATTTCGAAA GAATCAATTG TTGATGTTCG AGGCACTGTA AGAAAAGTGG AACAAGAAAT CATCAGCTGT ACACAGAGAG AAGTCGAAAT ACATGTTACA CAGTTGTTCG TTGTTAGCGC AGCAGAACCA CGCTTACCAC TGCAGATAGA AGATGCTGCT CGAATTAAAG AAGAAAGCAA AAGTGCAGAA GATTCATCTT TATCTATTGT GAATCTCGAC ACACGTTTAG ATAACAGAGT ACTGGATCTT CGTACGCAGA CAAGTCATGC TATTTTCTCT ATCCAAGCTG GTGTTTGTGA ATTGTTCCGA AACACTTTAA AGAAGCGTGG TTTCATGGAA ATACATACGC CAAAAATCAT ATCTGCAGCG AGCGAAGGAG GCGCAAATGT ATCTTATTTT AAAGGCTCTG CTTACCTTGC TCAGTCACCA CAGTTATATA AACAAATGGC AATCGCTGCA GATTTTGACA AAGTTTTCAC TATTGGTGCT GTATTTCGTG CAGAAGACAG CAATACTCAT CGGCATTTAA CAGAATTTGT CGGCCTTGAT ATTGAAATGG CGTTTAAGTT CCATTACCAT GAGGTTCTTG AAACGATTGG CGCTGTTCTG ATTGATATAT TCAAAGATTT GCAAGCGAAA TTCAAGCATG AAATCGAGAC AGTTGGCAAG CAGTATCCTT GTGAACCATT CGAATTCATT GAGCCTGCTC TTATACTCAA ATATCCAGAT GCAGTCAAAC TGTTACGAGA GAATGGTGTA GAAATAGGCG ATGAAGATGA TTTAAGCACT CCGGTAGAAA AATTTCTGGG AAGGTTGGTG AAAGAAAAAT ATCAGACCGA TTTTTACATA CTCGACAGAT ATCCACTAGC AGTTCGTCCA TTTTATACGA TGCCAGACGC AAACGATCCA AAATACTCCA ACAGTTATGA TATGTTTATG CGAGGTGAAG AAATATTATC TGGCGCTCAG CGAATCCATG ATGCATCTTT ATTAACTGAA AGAGCTAAAC ATCACCAAAT CGATTTGGAA AAGATCCGAG CTTATATTGA CTCGTTCAAG TATGGCTGCC CGCCACATGC AGGAGGTGGC ATCGGATTAG AACGTGTAAC GATGCTGTTT CTTGGACTTG GAAACGTCCG TTTGGCATCT CTTTTCCCGC GTGACCCTAA ACGAATAACA CCATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for OnvoA.00825.a.B7.PW38174
PURIFICATION DATe: 1/23/2017
CONCENTRATION: 20.2mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD GAEGEKKLSK KEINKLAKQQ KKMEKKAEHA APDVGENERE MEEDVSEGRY GAYGIIQSAE RKKLDFIDVK DVLVNLEGQE IWIRGRLHTS RIKGKTCFII IRQQMHSIQG MLFVGKEISK QMLKFVGNIS KESIVDVRGT VRKVEQEIIS CTQREVEIHV TQLFVVSAAE PRLPLQIEDA ARIKEESKSA EDSSLSIVNL DTRLDNRVLD LRTQTSHAIF SIQAGVCELF RNTLKKRGFM EIHTPKXISA ASEGGANVFE VSYFKGSAYL AQSPQLYKQM AIAADFDKVF TIGAVFRAED SNTHRHLTEF VGLDIEMAFK FHYHEVLETI GAVLIDIFKD LQAKFKHEIE TVGKQYPCEP FEFIEPALIL KYPDAVKLLR ENGVEIGDED DLSTPVEKFL GRLVKEKYQT DFYILDRYPL AVRPFYTMPD ANDPKYSNSY DMFMRGEEIL SGAQRIHDAS LLTERAKHHQ IDLEKIRAYI DSFKYGCPPH AGGGIGLERV TMLFLGLGNV RLASLFP
Validated NT Sequence
gcgggaaaag agatgccaaa cggacgtttc caagtccaag aaacagcatc gttacacgtt ctaatccgat gccacctcct gcatgtggcg ggcagccata cttgaacgag tcaatataag ctcggatctt ttccaaatcg atttggtgat gtttagctct ttcagttaat aaagatgcat catggattcg ctgagcgcca gataatattt cttcacctcg cataaacata tcataactgt tggagtattt tggatcgttt gcgtctggca tcgtataaaa tggacgaact gctagtggat atctgtcgag tatgtaaaaa tcggtctgat atttttcttt caccaacctt cccagaaatt tttctaccgg agtgcttaaa tcatcttcat cgcctatttc tacaccattc tctcgtaaca gtttgactgc atctggatat ttgagtataa gagcaggctc aatgaattcg aatggttcac aaggatactg cttgccaact gtctcgattt catgcttgaa tttcgcttgc aaatctttga atatatcaat cagaacagcg ccaatcgttt caagaacctc atggtaatgg aacttaaacg ccatttcaat atcaaggccg acaaattctg ttaaatgccg atgagtattg ctgtcttctg cacgaaatac agcaccaata gtgaaaactt tgtcaaaatc tgcagcgatt gccatttgtt tatataactg tggtgactga gcaaggtaag cagagccttt aaaataagat acctcaaaga catttgcgcc tccttcgctc gctgcagata tganttttgg cgtatgtatt tccatgaaac cacgcttctt taaagtgttt cggaacaatt cacaaacacc agcttggata gagaaaatag catgacttgt ctgcgtacga agatccagta ctctgttatc taaacgtgtg tcgagattca caatagataa agatgaatct tctgcacttt tgctttcttc tttaattcga gcagcatctt ctatctgcag tggtaagcgt ggttctgctg cgctaacaac gaacaactgt gtaacatgta tttcgacttc tctctgtgta cagctgatga tttcttgttc cacttttctt acagtgcctc gaacatcaac aattgattct ttcgaaatgt ttccaacaaa cttcaacatt tgtttgctaa tctctttacc aacgaacaac attccctgta tggaatgcat ttgctgacgg ataataatga agcacgtttt cccctttatc ctcgatgtgt gcaaacggcc ccgaatccaa atctcctggc cttctaaatt caccaagaca tctttaacat caatgaaatc caattttttt cgttcagcag attgtataat tccatatgca ccgtagcgac cttccgagac atcttcttcc atttctctct cattttcccc tacatctgga gctgcatgct cagccttctt ttccattttc ttttgttgct tcgccaattt gtttatttct ttttttgaca atttcttttc accttcagct ccatccatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMD GAEGEKKLSK KEINKLAKQQ KKMEKKAEHA APDVGENERE MEEDVSEGRY GAYGIIQSAE RKKLDFIDVK DVLVNLEGQE IWIRGRLHTS RIKGKTCFII IRQQMHSIQG MLFVGKEISK QMLKFVGNIS KESIVDVRGT VRKVEQEIIS CTQREVEIHV TQLFVVSAAE PRLPLQIEDA ARIKEESKSA EDSSLSIVNL DTRLDNRVLD LRTQTSHAIF SIQAGVCELF RNTLKKRGFM EIHTPKIISA ASEGGANVSY FKGSAYLAQS PQLYKQMAIA ADFDKVFTIG AVFRAEDSNT HRHLTEFVGL DIEMAFKFHY HEVLETIGAV LIDIFKDLQA KFKHEIETVG KQYPCEPFEF IEPALILKYP DAVKLLRENG VEIGDEDDLS TPVEKFLGRL VKEKYQTDFY ILDRYPLAVR PFYTMPDAND PKYSNSYDMF MRGEEILSGA QRIHDASLLT ERAKHHQIDL EKIRAYIDSF KYGCPPHAGG GIGLERVTML FLGLGNVRLA SLFPRDPKRI TP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATG GAGCTGAAGG TGAAAAGAAA TTGTCAAAAA AAGAAATAAA CAAATTGGCG AAGCAACAAA AGAAAATGGA AAAGAAGGCT GAGCATGCAG CTCCAGATGT AGGGGAAAAT GAGAGAGAAA TGGAAGAAGA TGTCTCGGAA GGTCGCTACG GTGCATATGG AATTATACAA TCTGCTGAAC GAAAAAAATT GGATTTCATT GATGTTAAAG ATGTCTTGGT GAATTTAGAA GGCCAGGAGA TTTGGATTCG GGGCCGTTTG CACACATCGA GGATAAAGGG GAAAACGTGC TTCATTATTA TCCGTCAGCA AATGCATTCC ATACAGGGAA TGTTGTTCGT TGGTAAAGAG ATTAGCAAAC AAATGTTGAA GTTTGTTGGA AACATTTCGA AAGAATCAAT TGTTGATGTT CGAGGCACTG TAAGAAAAGT GGAACAAGAA ATCATCAGCT GTACACAGAG AGAAGTCGAA ATACATGTTA CACAGTTGTT CGTTGTTAGC GCAGCAGAAC CACGCTTACC ACTGCAGATA GAAGATGCTG CTCGAATTAA AGAAGAAAGC AAAAGTGCAG AAGATTCATC TTTATCTATT GTGAATCTCG ACACACGTTT AGATAACAGA GTACTGGATC TTCGTACGCA GACAAGTCAT GCTATTTTCT CTATCCAAGC TGGTGTTTGT GAATTGTTCC GAAACACTTT AAAGAAGCGT GGTTTCATGG AAATACATAC GCCAAAAATC ATATCTGCAG CGAGCGAAGG AGGCGCAAAT GTATCTTATT TTAAAGGCTC TGCTTACCTT GCTCAGTCAC CACAGTTATA TAAACAAATG GCAATCGCTG CAGATTTTGA CAAAGTTTTC ACTATTGGTG CTGTATTTCG TGCAGAAGAC AGCAATACTC ATCGGCATTT AACAGAATTT GTCGGCCTTG ATATTGAAAT GGCGTTTAAG TTCCATTACC ATGAGGTTCT TGAAACGATT GGCGCTGTTC TGATTGATAT ATTCAAAGAT TTGCAAGCGA AATTCAAGCA TGAAATCGAG ACAGTTGGCA AGCAGTATCC TTGTGAACCA TTCGAATTCA TTGAGCCTGC TCTTATACTC AAATATCCAG ATGCAGTCAA ACTGTTACGA GAGAATGGTG TAGAAATAGG CGATGAAGAT GATTTAAGCA CTCCGGTAGA AAAATTTCTG GGAAGGTTGG TGAAAGAAAA ATATCAGACC GATTTTTACA TACTCGACAG ATATCCACTA GCAGTTCGTC CATTTTATAC GATGCCAGAC GCAAACGATC CAAAATACTC CAACAGTTAT GATATGTTTA TGCGAGGTGA AGAAATATTA TCTGGCGCTC AGCGAATCCA TGATGCATCT TTATTAACTG AAAGAGCTAA ACATCACCAA ATCGATTTGG AAAAGATCCG AGCTTATATT GACTCGTTCA AGTATGGCTG CCCGCCACAT GCAGGAGGTG GCATCGGATT AGAACGTGTA ACGATGCTGT TTCTTGGACT TGGAAACGTC CGTTTGGCAT CTCTTTTCCC GCGTGACCCT AAACGAATAA CACCATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for OnvoA.00825.a.B1.PW38178
PURIFICATION DATe: 2/7/2017
CONCENTRATION: 28mg/ml
OBSERVED MW: 61kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MTTEDGVSMD GAEGEKKLSK KEINKLAKQQ KKMEKKAEHA APDVGENERE MEEDVSEGRY GAYGIIQSAE RKKLDFIDVK DVLVNLEGQE IWIRGRLHTS RIKGKTCFII IRQQMHSIQG MLFVGKEISK QMLKFVGNIS KESIVDVRGT VRKVEQEIIS CTQREVEIHV TQLFVVSAAE PRLPLQIEDA ARIKEESKSA EDSSLSIVNL DTRLDNRVLD LRTQTSHAIF SIQAGVCELF RNTLKKRGFM EIHTPKIISA ASEGGANVFE VSYFKGSAYL AQSPQLYKQM AIAADFDKVF TIGAVFRAED SNTHRHLTEF VGLDIEMAFK FHYHEVLETI GAVLIDIFKD LQAKFKHEIE TVGKQYPCEP FEFIEPALIL KYPDAVKLLR ENGVEIGDED DLSTPVEKFL GRLVKEKYQT DFYILDRYPL AVRPFYTMPD ANDPKYSNSY DMFMRGEEIL SGAQRIHDAS LLTERAKHHQ IDLEKIRAYI DSFKYGCPPH AGGGIGLERV TMLFLGLGNV RLASLF
Validated NT Sequence
cgggaaaaga gatgccaaac ggacgtttcc aagtccaaga aacagcatcg ttacacgttc taatccgatg ccacctcctg catgtggcgg gcagccatac ttgaacgagt caatataagc tcggatcttt tccaaatcga tttggtgatg tttagctctt tcagttaata aagatgcatc atggattcgc tgagcgccag ataatatttc ttcacctcgc ataaacatat cataactgtt ggagtatttt ggatcgtttg cgtctggcat cgtataaaat ggacgaactg ctagtggata tctgtcgagt atgtaaaaat cggtctgata tttttctttc accaaccttc ccagaaattt ttctaccgga gtgcttaaat catcttcatc gcctatttct acaccattct ctcgtaacag tttgactgca tctggatatt tgagtataag agcaggctca atgaattcga atggttcaca aggatactgc ttgccaactg tctcgatttc atgcttgaat ttcgcttgca aatctttgaa tatatcaatc agaacagcgc caatcgtttc aagaacctca tggtaatgga acttaaacgc catttcaata tcaaggccga caaattctgt taaatgccga tgagtattgc tgtcttctgc acgaaataca gcaccaatag tgaaaacttt gtcaaaatct gcagcgattg ccatttgttt atataactgt ggtgactgag caaggtaagc agagccttta aaataagata cctcaaagac atttgcgcct ccttcgctcg ctgcagatat gatttttggc gtatgtattt ccatgaaacc acgcttcttt aaagtgtttc ggaacaattc acaaacacca gcttggatag agaaaatagc atgacttgtc tgcgtacgaa gatccagtac tctgttatct aaacgtgtgt cgagattcac aatagataaa gatgaatctt ctgcactttt gctttcttct ttaattcgag cagcatcttc tatctgcagt ggtaagcgtg gttctgctgc gctaacaacg aacaactgtg taacatgtat ttcgacttct ctctgtgtac agctgatgat ttcttgttcc acttttctta cagtgcctcg aacatcaaca attgattctt tcgaaatgtt tccaacaaac ttcaacattt gtttgctaat ctctttacca acgaacaaca ttccctgtat ggaatgcatt tgctgacgga taataatgaa gcacgttttc ccctttatcc tcgatgtgtg caaacggccc cgaatccaaa tctcctggcc ttctaaattc accaagacat ctttaacatc aatgaaatcc aatttttttc gttcagcaga ttgtataatt ccatatgcac cgtagcgacc ttccgagaca tcttcttcca tttctctctc attttcccct acatctggag ctgcatgctc agccttcttt tccattttct tttgttgctt cgccaatttg tttatttctt tttttgacaa tttcttttca ccttcagctc catccatact tactccatct tcagttgtca tatggtggtg gtggnnnt
Expressed Protein Sequence
MAHHHHHHMT TEDGVSMDGA EGEKKLSKKE INKLAKQQKK MEKKAEHAAP DVGENEREME EDVSEGRYGA YGIIQSAERK KLDFIDVKDV LVNLEGQEIW IRGRLHTSRI KGKTCFIIIR QQMHSIQGML FVGKEISKQM LKFVGNISKE SIVDVRGTVR KVEQEIISCT QREVEIHVTQ LFVVSAAEPR LPLQIEDAAR IKEESKSAED SSLSIVNLDT RLDNRVLDLR TQTSHAIFSI QAGVCELFRN TLKKRGFMEI HTPKIISAAS EGGANVSYFK GSAYLAQSPQ LYKQMAIAAD FDKVFTIGAV FRAEDSNTHR HLTEFVGLDI EMAFKFHYHE VLETIGAVLI DIFKDLQAKF KHEIETVGKQ YPCEPFEFIE PALILKYPDA VKLLRENGVE IGDEDDLSTP VEKFLGRLVK EKYQTDFYIL DRYPLAVRPF YTMPDANDPK YSNSYDMFMR GEEILSGAQR IHDASLLTER AKHHQIDLEK IRAYIDSFKY GCPPHAGGGI GLERVTMLFL GLGNVRLASL FPRDPKRITP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAA CTGAAGATGG AGTAAGTATG GATGGAGCTG AAGGTGAAAA GAAATTGTCA AAAAAAGAAA TAAACAAATT GGCGAAGCAA CAAAAGAAAA TGGAAAAGAA GGCTGAGCAT GCAGCTCCAG ATGTAGGGGA AAATGAGAGA GAAATGGAAG AAGATGTCTC GGAAGGTCGC TACGGTGCAT ATGGAATTAT ACAATCTGCT GAACGAAAAA AATTGGATTT CATTGATGTT AAAGATGTCT TGGTGAATTT AGAAGGCCAG GAGATTTGGA TTCGGGGCCG TTTGCACACA TCGAGGATAA AGGGGAAAAC GTGCTTCATT ATTATCCGTC AGCAAATGCA TTCCATACAG GGAATGTTGT TCGTTGGTAA AGAGATTAGC AAACAAATGT TGAAGTTTGT TGGAAACATT TCGAAAGAAT CAATTGTTGA TGTTCGAGGC ACTGTAAGAA AAGTGGAACA AGAAATCATC AGCTGTACAC AGAGAGAAGT CGAAATACAT GTTACACAGT TGTTCGTTGT TAGCGCAGCA GAACCACGCT TACCACTGCA GATAGAAGAT GCTGCTCGAA TTAAAGAAGA AAGCAAAAGT GCAGAAGATT CATCTTTATC TATTGTGAAT CTCGACACAC GTTTAGATAA CAGAGTACTG GATCTTCGTA CGCAGACAAG TCATGCTATT TTCTCTATCC AAGCTGGTGT TTGTGAATTG TTCCGAAACA CTTTAAAGAA GCGTGGTTTC ATGGAAATAC ATACGCCAAA AATCATATCT GCAGCGAGCG AAGGAGGCGC AAATGTATCT TATTTTAAAG GCTCTGCTTA CCTTGCTCAG TCACCACAGT TATATAAACA AATGGCAATC GCTGCAGATT TTGACAAAGT TTTCACTATT GGTGCTGTAT TTCGTGCAGA AGACAGCAAT ACTCATCGGC ATTTAACAGA ATTTGTCGGC CTTGATATTG AAATGGCGTT TAAGTTCCAT TACCATGAGG TTCTTGAAAC GATTGGCGCT GTTCTGATTG ATATATTCAA AGATTTGCAA GCGAAATTCA AGCATGAAAT CGAGACAGTT GGCAAGCAGT ATCCTTGTGA ACCATTCGAA TTCATTGAGC CTGCTCTTAT ACTCAAATAT CCAGATGCAG TCAAACTGTT ACGAGAGAAT GGTGTAGAAA TAGGCGATGA AGATGATTTA AGCACTCCGG TAGAAAAATT TCTGGGAAGG TTGGTGAAAG AAAAATATCA GACCGATTTT TACATACTCG ACAGATATCC ACTAGCAGTT CGTCCATTTT ATACGATGCC AGACGCAAAC GATCCAAAAT ACTCCAACAG TTATGATATG TTTATGCGAG GTGAAGAAAT ATTATCTGGC GCTCAGCGAA TCCATGATGC ATCTTTATTA ACTGAAAGAG CTAAACATCA CCAAATCGAT TTGGAAAAGA TCCGAGCTTA TATTGACTCG TTCAAGTATG GCTGCCCGCC ACATGCAGGA GGTGGCATCG GATTAGAACG TGTAACGATG CTGTTTCTTG GACTTGGAAA CGTCCGTTTG GCATCTCTTT TCCCGCGTGA CCCTAAACGA ATAACACCAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT